Rmu_sc0020346.1_g000001

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020346.1
OG0012356
Physical Location & Seq
Reverse (-)
517 .. 1929
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020346.1_g000001.1.cds

Sequence Viewer

Length: 579 bp
atgccggggatgcaaggatggcaggcggtggcgtttcggatatcaggcgacaaggcatacttctccggatgtgggttctatggcgcccaagacacgctctgcgacgatgtcggacgccattacttcaaggagtgttacatcgagggctccatagacttcatctttggaaatggccgctccatgtacaaagactgtgagctgcactcaatagctaccaagttcggctccattgcggctcagttcagaaaatcaccggacgacaaatcgggcttcgcttttctcaactgccgggtaacgggttcgggtccactctacgtgggtcgggccatgggcaagtacgccaggattgtatactcctacacatactttgatcatgtggtcgcacatggtgcctgggatgactggaacacaaccagtacaaagactgattttctaggagtgtacaagtgctggggtcctggagcagaagccttgcgcggactgtcatatgcaccggagcttgatttcgacttggcgcacccatttctgaggaagagctttgtcaatggaagacactggatagccccatctgatgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

192

Amino Acids

21.44

Weight (kDa)

6.99

Isoelectric Point (pI)

28.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47500
fragaria_vesca FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880
malus_domestica MD06G1058600.v1.1
prunus_persica Prupe.2G003200_v2.0.a1 Prupe.5G069600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0203261
rosa_laevigata RLG00000003539
rosa_multiflora Rmu_sc0020346.1_g000001
rosa_roxburghii Rroxscaffold_3G00253900
rosa_rugosa Rorug07G0075400
rosa_samantha Rh7AG204900 Rh7BG204300 Rh7CG215900 Rh7DG211500
rosa_wichuraiana Rw7G017840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 83, 389
AccBSI CCGCTC 1 cut(s) 177
AccI GTMKAC 1 cut(s) 351
AccII CGCG 1 cut(s) 477
AccIII TCCGGA 1 cut(s) 65
AciI CCGC 4 cut(s) 26, 175, 233, 477
AcoI YGGCCR 1 cut(s) 172
AcyI GRCGYC 2 cut(s) 84, 115
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 4 cut(s) 185, 338, 418, 443
AfiI CCNNNNNNNGG 2 cut(s) 72, 295
AgsI TTSAA 1 cut(s) 127
AjnI CCWGG 3 cut(s) 341, 392, 457
AluBI AGCT 4 cut(s) 199, 212, 499, 537
AluI AGCT 4 cut(s) 199, 212, 499, 537
Aor13HI TCCGGA 1 cut(s) 65
AoxI GGCC 2 cut(s) 172, 324
ApeKI GCWGC 1 cut(s) 199
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspLEI GCGC 3 cut(s) 86, 477, 517
AspS9I GGNCC 3 cut(s) 305, 324, 455
AsuC2I CCSGG 2 cut(s) 6, 290
AsuHPI GGTGA 1 cut(s) 243
AvaII GGWCC 2 cut(s) 305, 455
BanI GGYRCC 2 cut(s) 83, 389
BanII GRGCYC 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 556
BbvI GCAGC 1 cut(s) 186
BccI CCATC 2 cut(s) 12, 574
BciT130I CCWGG 3 cut(s) 343, 394, 459
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 2 cut(s) 6, 290
BfaI CTAG 1 cut(s) 434
BfoI RGCGCY 1 cut(s) 87
BisI GCNGC 3 cut(s) 175, 200, 234
BlsI GCNGC 3 cut(s) 176, 201, 235
Bme1390I CCNGG 5 cut(s) 6, 290, 343, 394, 459
Bme18I GGWCC 2 cut(s) 305, 455
BmgT120I GGNCC 3 cut(s) 305, 324, 455
BmiI GGNNCC 6 cut(s) 85, 148, 226, 306, 391, 456
BmrFI CCNGG 5 cut(s) 6, 290, 343, 394, 459
BmsI GCATC 1 cut(s) 562
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 2 cut(s) 188, 220
BpmI CTGGAG 1 cut(s) 480
BpuMI CCSGG 2 cut(s) 6, 290
BsaAI YACGTR 1 cut(s) 316
BsaHI GRCGYC 2 cut(s) 84, 115
BsaJI CCNNGG 3 cut(s) 5, 327, 393
BsaWI WCCGGW 3 cut(s) 65, 253, 493
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 295
Bse1I ACTGG 3 cut(s) 407, 414, 560
Bse3DI GCAATG 1 cut(s) 228
BseAI TCCGGA 1 cut(s) 65
BseBI CCWGG 3 cut(s) 343, 394, 459
BseDI CCNNGG 3 cut(s) 5, 327, 393
BseGI GGATG 4 cut(s) 15, 23, 74, 403
BseLI CCNNNNNNNGG 2 cut(s) 72, 295
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 251, 518
BseNI ACTGG 3 cut(s) 407, 414, 560
BseXI GCAGC 1 cut(s) 186
BseYI CCCAGC 1 cut(s) 450
BsgI GTGCAG 1 cut(s) 185
Bsh1236I CGCG 1 cut(s) 477
BshFI GGCC 2 cut(s) 174, 326
BshNI GGYRCC 2 cut(s) 83, 389
BsiSI CCGG 5 cut(s) 5, 66, 254, 289, 494
BslI CCNNNNNNNGG 2 cut(s) 72, 295
BsnI GGCC 2 cut(s) 174, 326
Bsp1286I GDGCHC 1 cut(s) 149
Bsp13I TCCGGA 1 cut(s) 65
Bsp1407I TGTACA 2 cut(s) 183, 441
Bsp143I GATC 1 cut(s) 370
Bsp19I CCATGG 1 cut(s) 327
BspACI CCGC 4 cut(s) 26, 175, 233, 477
BspANI GGCC 2 cut(s) 174, 326
BspCNI CTCAG 2 cut(s) 250, 519
BspEI TCCGGA 1 cut(s) 65
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 6 cut(s) 85, 148, 226, 306, 391, 456
BspQI GCTCTTC 1 cut(s) 527
BspT107I GGYRCC 2 cut(s) 83, 389
BsrBI CCGCTC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 228
BsrGI TGTACA 2 cut(s) 183, 441
BsrI ACTGG 3 cut(s) 407, 414, 560
BssECI CCNNGG 3 cut(s) 5, 327, 393
BssMI GATC 1 cut(s) 370
BssNAI GTATAC 1 cut(s) 352
BssNI GRCGYC 2 cut(s) 84, 115
BssT1I CCWWGG 1 cut(s) 327
Bst1107I GTATAC 1 cut(s) 352
Bst2UI CCWGG 3 cut(s) 343, 394, 459
Bst4CI ACNGT 2 cut(s) 194, 483
Bst6I CTCTTC 1 cut(s) 527
BstACI GRCGYC 2 cut(s) 84, 115
BstAPI GCANNNNNTGC 1 cut(s) 389
BstAUI TGTACA 2 cut(s) 183, 441
BstBAI YACGTR 1 cut(s) 316
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 2 cut(s) 237, 527
BstDSI CCRYGG 1 cut(s) 327
BstF5I GGATG 4 cut(s) 15, 23, 74, 403
BstFNI CGCG 1 cut(s) 477
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 3 cut(s) 86, 477, 517
BstKTI GATC 1 cut(s) 373
BstMBI GATC 1 cut(s) 370
BstMWI GCNNNNNNNGC 3 cut(s) 10, 19, 389
BstNI CCWGG 3 cut(s) 343, 394, 459
BstSCI CCNGG 5 cut(s) 4, 288, 341, 392, 457
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 186
BstV2I GAAGAC 1 cut(s) 556
BstZ17I GTATAC 1 cut(s) 352
BsuRI GGCC 2 cut(s) 174, 326
BtgI CCRYGG 1 cut(s) 327
BtsCI GGATG 4 cut(s) 15, 23, 74, 403
BtsIMutI CAGTG 1 cut(s) 553
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 3 cut(s) 86, 477, 517
Cfr13I GGNCC 3 cut(s) 305, 324, 455
CseI GACGC 1 cut(s) 123
Csp6I GTAC 4 cut(s) 184, 337, 417, 442
CviAII CATG 4 cut(s) 181, 328, 374, 386
CviQI GTAC 4 cut(s) 184, 337, 417, 442
DdeI CTNAG 2 cut(s) 237, 527
DinI GGCGCC 1 cut(s) 85
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
DraIII CACNNNGTG 1 cut(s) 389
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 527
EarI CTCTTC 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 149
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 2 cut(s) 305, 455
EcoO109I RGGNCCY 1 cut(s) 455
EcoRII CCWGG 3 cut(s) 341, 392, 457
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 1 cut(s) 149
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 4 cut(s) 184, 331, 377, 389
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FatI CATG 4 cut(s) 180, 327, 373, 385
FauNDI CATATG 1 cut(s) 487
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 3 cut(s) 175, 200, 234
FokI GGATG 4 cut(s) 22, 30, 81, 410
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 3 cut(s) 175, 200, 234
FspBI CTAG 1 cut(s) 434
GlaI GCGC 3 cut(s) 85, 476, 516
GluI GCNGC 3 cut(s) 175, 200, 234
GsaI CCCAGC 1 cut(s) 454
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 2 cut(s) 174, 326
HapII CCGG 5 cut(s) 5, 66, 254, 289, 494
HgaI GACGC 1 cut(s) 123
HhaI GCGC 3 cut(s) 86, 477, 517
Hin1I GRCGYC 2 cut(s) 84, 115
Hin1II CATG 4 cut(s) 184, 331, 377, 389
Hin6I GCGC 3 cut(s) 84, 475, 515
HinP1I GCGC 3 cut(s) 84, 475, 515
HpaII CCGG 5 cut(s) 5, 66, 254, 289, 494
HphI GGTGA 1 cut(s) 243
Hpy166II GTNNAC 3 cut(s) 308, 352, 442
Hpy188I TCNGA 5 cut(s) 39, 113, 245, 528, 571
Hpy188III TCNNGA 1 cut(s) 66
Hpy8I GTNNAC 3 cut(s) 308, 352, 442
Hpy99I CGWCG 1 cut(s) 107
HpyCH4III ACNGT 2 cut(s) 194, 483
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 3 cut(s) 13, 202, 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 19, 389
HpyF3I CTNAG 2 cut(s) 237, 527
HpySE526I ACGT 1 cut(s) 315
Hsp92I GRCGYC 2 cut(s) 84, 115
Hsp92II CATG 4 cut(s) 184, 331, 377, 389
HspAI GCGC 3 cut(s) 84, 475, 515
KasI GGCGCC 1 cut(s) 83
Kpn2I TCCGGA 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 1 cut(s) 370
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 5 cut(s) 152, 182, 230, 461, 496
Lsp1109I GCAGC 1 cut(s) 186
LweI GCATC 1 cut(s) 562
MaeI CTAG 1 cut(s) 434
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 2 cut(s) 134, 292
MalI GATC 1 cut(s) 372
MbiI CCGCTC 1 cut(s) 177
MboI GATC 1 cut(s) 370
MboII GAAGA 2 cut(s) 544, 561
MhlI GDGCHC 1 cut(s) 149
Mly113I GGCGCC 1 cut(s) 84
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 2 cut(s) 136, 522
MroI TCCGGA 1 cut(s) 65
MseI TTAA 1 cut(s) 577
MspI CCGG 5 cut(s) 5, 66, 254, 289, 494
MspR9I CCNGG 5 cut(s) 6, 290, 343, 394, 459
MvaI CCWGG 3 cut(s) 343, 394, 459
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 3 cut(s) 10, 19, 389
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 2 cut(s) 6, 290
NcoI CCATGG 1 cut(s) 327
NdeI CATATG 1 cut(s) 487
NdeII GATC 1 cut(s) 370
NlaIII CATG 4 cut(s) 184, 331, 377, 389
NlaIV GGNNCC 6 cut(s) 85, 148, 226, 306, 391, 456
PciSI GCTCTTC 1 cut(s) 527
PflFI GACNNNGTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 457
PkrI GCNGC 3 cut(s) 176, 201, 235
PluTI GGCGCC 1 cut(s) 87
Ppu21I YACGTR 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 455
Psp6I CCWGG 3 cut(s) 341, 392, 457
PspFI CCCAGC 1 cut(s) 450
PspGI CCWGG 3 cut(s) 341, 392, 457
PspN4I GGNNCC 6 cut(s) 85, 148, 226, 306, 391, 456
PspPI GGNCC 3 cut(s) 305, 324, 455
PspPPI RGGWCCY 1 cut(s) 455
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 4 cut(s) 185, 338, 418, 443
RsaNI GTAC 4 cut(s) 184, 337, 417, 442
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 1 cut(s) 577
SatI GCNGC 3 cut(s) 175, 200, 234
Sau3AI GATC 1 cut(s) 370
Sau96I GGNCC 3 cut(s) 305, 324, 455
ScrFI CCNGG 5 cut(s) 6, 290, 343, 394, 459
SduI GDGCHC 1 cut(s) 149
SetI ASST 5 cut(s) 201, 214, 318, 501, 539
SfaNI GCATC 1 cut(s) 562
SfoI GGCGCC 1 cut(s) 85
SinI GGWCC 2 cut(s) 305, 455
SsiI CCGC 4 cut(s) 26, 175, 233, 477
SspDI GGCGCC 1 cut(s) 83
SspMI CTAG 1 cut(s) 434
StyD4I CCNGG 5 cut(s) 4, 288, 341, 392, 457
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 194, 483
TaiI ACGT 1 cut(s) 318
TaqI TCGA 2 cut(s) 141, 507
TatI WGTACW 3 cut(s) 183, 416, 441
TauI GCSGC 2 cut(s) 177, 236
Tru1I TTAA 1 cut(s) 577
Tru9I TTAA 1 cut(s) 577
TscAI CASTG 1 cut(s) 560
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 1 cut(s) 148
TspRI CASTG 1 cut(s) 560
Tth111I GACNNNGTC 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 305, 455
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.