MD06G1058600.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012356
Physical Location & Seq
Forward (+)
9088559 .. 9092048
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1058600.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGGCTTCGCATTCTCTTGTGTTATCATTCATTTGTTTCTACTTGGTGTGCAACATACTTACTTTGTTAATGTTGTTTCATGCAGTGCCAATTGTGACGGCAGCGAGAAGCTCTCACGTACTAGTCCGCAGCAGTCTGATGACCTCGTCACCAACCCCTGCGACCGATAGCAGCAGTTCCAAGCCCCACCACAAGTGGGTTGGACCCAGCGGCCACCGCCTGATCATGGTCGACGTGAACGGCTCCGGGGACTTCCAGTCTGTCCAGTCCGCCGTCAATGCAGTCCCGGTGAACAACACGGTCAATATTGTCATCCTAATCAAGCCCGGATGTTACATAGAGAAAGTGGTAGTGCCGGCGACGAAGCCGTACATCACGTTTCAAGGAGCCGGGAAAGATGCGACGGTGATCGAATGGCATGACCGTGCCAGTGACCCTGGACCCAACGGCCAGCAGCTCAGAACTTACCGAACGGCTTCTGTTACTGTTTTTGCTAACTATTTCTCTGCCAGAAATATTAGCTTCAAGAATACTGCACCAGCACCGATGCCGGGGATGCAAGGATGGCAAGCGGTGGCATTTCGGATATCCGGCGACAAGGCGTACTTCTCAGGCTGTGGCTTCTATGGTGCCCAAGACACACTCTGTGACGACGTAGGACGCCATTACTTCAAGGAGTGTTACATTGAGGGTTCCATAGACTTCATTTTTGGGAATGGTCGGTCCATGTACAAAGACTGTGAGCTGCACTCGATAGCAACCAAGTTCGGGTCCATTGCAGCCCACTACAGGAACTCGCCGGATGATAAGTCAGGGTTCGCTTTCCTGAACTGCCGGGTTACCGGTACGGGTCCGTTGTACGTGGGTCGGGCCATGGGGAAGTACTCCCGGATCGTATACTCCTACACCTACTTTGATGACGTGGTTGCACACGGTGCCTGGGATGACTGGAACAACACAATCACAAGTACTGCTACAAATGGGAGTGTGTATCTGGGAGTGTACAAGTGCTGGGGGCCAGGAGCAGAAGCCCTGCGAGGGGTGCCATGTGCCCCGGAGCTTGAGTACGACTTGGCTCATCCATTCCTCGCAAAGAGCTTCGTCAATGGCAGGCACTGGATCGCACCCGATGATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

380

Amino Acids

41.41

Weight (kDa)

7.99

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 75 - 369 4.7e-55 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47500
fragaria_vesca FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880
malus_domestica MD06G1058600.v1.1
prunus_persica Prupe.2G003200_v2.0.a1 Prupe.5G069600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0203261
rosa_laevigata RLG00000003539
rosa_multiflora Rmu_sc0020346.1_g000001
rosa_roxburghii Rroxscaffold_3G00253900
rosa_rugosa Rorug07G0075400
rosa_samantha Rh7AG204900 Rh7BG204300 Rh7CG215900 Rh7DG211500
rosa_wichuraiana Rw7G017840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 629, 935, 1042
AccI GTMKAC 2 cut(s) 231, 897
AciI CCGC 5 cut(s) 127, 210, 217, 270, 572
AclWI GGATC 2 cut(s) 899, 1127
AcoI YGGCCR 2 cut(s) 211, 448
AcyI GRCGYC 1 cut(s) 661
AdeI CACNNNGTG 2 cut(s) 647, 935
AfiI CCNNNNNNNGG 7 cut(s) 196, 226, 551, 768, 789, 1038, 1039
AgeI ACCGGT 1 cut(s) 842
AgsI TTSAA 3 cut(s) 383, 526, 673
AhdI GACNNNNNGTC 1 cut(s) 256
AhlI ACTAGT 1 cut(s) 121
AjiI CACGTC 2 cut(s) 235, 922
AjnI CCWGG 3 cut(s) 436, 938, 1018
AluBI AGCT 6 cut(s) 111, 457, 522, 745, 1060, 1098
AluI AGCT 6 cut(s) 111, 457, 522, 745, 1060, 1098
AlwI GGATC 2 cut(s) 899, 1127
AlwNI CAGNNNCTG 1 cut(s) 1116
AoxI GGCC 4 cut(s) 211, 448, 870, 1016
ApeKI GCWGC 6 cut(s) 101, 129, 171, 454, 745, 779
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
AsiGI ACCGGT 1 cut(s) 842
Asp700I GAANNNNTTC 1 cut(s) 475
AspS9I GGNCC 7 cut(s) 203, 440, 723, 771, 851, 870, 1016
AsuC2I CCSGG 8 cut(s) 247, 287, 327, 391, 552, 836, 889, 1055
AsuHPI GGTGA 3 cut(s) 141, 301, 418
AvaII GGWCC 5 cut(s) 203, 440, 723, 771, 851
BaeGI GKGCMC 2 cut(s) 634, 1054
BanI GGYRCC 3 cut(s) 629, 935, 1042
BbvI GCAGC 6 cut(s) 113, 141, 183, 466, 732, 791
BccI CCATC 1 cut(s) 558
BceAI ACGGC 6 cut(s) 114, 256, 257, 352, 463, 489
BciT130I CCWGG 3 cut(s) 438, 940, 1020
BclI TGATCA 1 cut(s) 222
BcnI CCSGG 8 cut(s) 247, 287, 327, 391, 552, 836, 889, 1055
BcuI ACTAGT 1 cut(s) 121
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 1 cut(s) 787
BisI GCNGC 7 cut(s) 102, 130, 172, 211, 455, 746, 780
BlsI GCNGC 7 cut(s) 103, 131, 173, 212, 456, 747, 781
BmcAI AGTACT 2 cut(s) 884, 970
Bme18I GGWCC 5 cut(s) 203, 440, 723, 771, 851
BmeRI GACNNNNNGTC 1 cut(s) 256
BmgBI CACGTC 2 cut(s) 235, 922
BmgT120I GGNCC 7 cut(s) 203, 440, 723, 771, 851, 870, 1016
BmsI GCATC 4 cut(s) 388, 537, 546, 1123
BplI GAGNNNNNCTC 4 cut(s) 97, 129, 734, 766
BpuEI CTTGAG 1 cut(s) 1082
BpuMI CCSGG 8 cut(s) 247, 287, 327, 391, 552, 836, 889, 1055
BsaAI YACGTR 2 cut(s) 118, 862
BsaHI GRCGYC 1 cut(s) 661
BsaJI CCNNGG 6 cut(s) 246, 436, 551, 873, 939, 1053
BsaWI WCCGGW 1 cut(s) 842
BsaXI ACNNNNNCTCC 2 cut(s) 1049, 1079
Bsc4I CCNNNNNNNGG 7 cut(s) 196, 226, 551, 768, 789, 1038, 1039
Bse118I RCCGGY 2 cut(s) 355, 842
Bse1I ACTGG 5 cut(s) 256, 265, 429, 953, 1121
Bse3DI GCAATG 1 cut(s) 774
BseBI CCWGG 3 cut(s) 438, 940, 1020
BseDI CCNNGG 6 cut(s) 246, 436, 551, 873, 939, 1053
BseGI GGATG 7 cut(s) 312, 335, 561, 569, 808, 949, 1078
BseLI CCNNNNNNNGG 7 cut(s) 196, 226, 551, 768, 789, 1038, 1039
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 2 cut(s) 472, 624
BseNI ACTGG 5 cut(s) 256, 265, 429, 953, 1121
BseSI GKGCMC 2 cut(s) 634, 1054
BseXI GCAGC 6 cut(s) 113, 141, 183, 466, 732, 791
BseYI CCCAGC 2 cut(s) 206, 1011
BsgI GTGCAG 2 cut(s) 519, 731
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 4 cut(s) 213, 450, 872, 1018
BshNI GGYRCC 3 cut(s) 629, 935, 1042
BshTI ACCGGT 1 cut(s) 842
BsiEI CGRYCG 1 cut(s) 165
BslFI GGGAC 2 cut(s) 263, 269
BslI CCNNNNNNNGG 7 cut(s) 196, 226, 551, 768, 789, 1038, 1039
BsmFI GGGAC 2 cut(s) 263, 269
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 4 cut(s) 213, 450, 872, 1018
Bsp1286I GDGCHC 2 cut(s) 634, 1054
Bsp1407I TGTACA 2 cut(s) 729, 1002
Bsp143I GATC 4 cut(s) 222, 408, 891, 1119
Bsp19I CCATGG 1 cut(s) 873
BspACI CCGC 5 cut(s) 127, 210, 217, 270, 572
BspANI GGCC 4 cut(s) 213, 450, 872, 1018
BspCNI CTCAG 2 cut(s) 471, 623
BspPI GGATC 2 cut(s) 899, 1127
BspT107I GGYRCC 3 cut(s) 629, 935, 1042
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 2 cut(s) 355, 842
BsrGI TGTACA 2 cut(s) 729, 1002
BsrI ACTGG 5 cut(s) 256, 265, 429, 953, 1121
BssAI RCCGGY 2 cut(s) 355, 842
BssECI CCNNGG 6 cut(s) 246, 436, 551, 873, 939, 1053
BssMI GATC 4 cut(s) 222, 408, 891, 1119
BssNAI GTATAC 1 cut(s) 898
BssNI GRCGYC 1 cut(s) 661
BssT1I CCWWGG 1 cut(s) 873
Bst1107I GTATAC 1 cut(s) 898
Bst2UI CCWGG 3 cut(s) 438, 940, 1020
Bst4CI ACNGT 6 cut(s) 301, 406, 425, 487, 740, 935
BstACI GRCGYC 1 cut(s) 661
BstAPI GCANNNNNTGC 1 cut(s) 935
BstAUI TGTACA 2 cut(s) 729, 1002
BstBAI YACGTR 2 cut(s) 118, 862
BstC8I GCNNGC 4 cut(s) 357, 452, 570, 1112
BstDEI CTNAG 2 cut(s) 458, 610
BstDSI CCRYGG 1 cut(s) 873
BstEII GGTNACC 1 cut(s) 838
BstF5I GGATG 7 cut(s) 312, 335, 561, 569, 808, 949, 1078
BstKTI GATC 4 cut(s) 225, 411, 894, 1122
BstMBI GATC 4 cut(s) 222, 408, 891, 1119
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 6 cut(s) 216, 278, 556, 565, 935, 1042
BstNI CCWGG 3 cut(s) 438, 940, 1020
BstPI GGTNACC 1 cut(s) 838
BstSFI CTRYAG 1 cut(s) 787
BstSLI GKGCMC 2 cut(s) 634, 1054
BstV1I GCAGC 6 cut(s) 113, 141, 183, 466, 732, 791
BstZ17I GTATAC 1 cut(s) 898
BsuRI GGCC 4 cut(s) 213, 450, 872, 1018
BtgI CCRYGG 1 cut(s) 873
BtrI CACGTC 2 cut(s) 235, 922
BtsCI GGATG 7 cut(s) 312, 335, 561, 569, 808, 949, 1078
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 3 cut(s) 90, 436, 1114
Cac8I GCNNGC 4 cut(s) 357, 452, 570, 1112
CaiI CAGNNNCTG 1 cut(s) 1116
Cfr10I RCCGGY 2 cut(s) 355, 842
Cfr13I GGNCC 7 cut(s) 203, 440, 723, 771, 851, 870, 1016
CseI GACGC 1 cut(s) 669
CspAI ACCGGT 1 cut(s) 842
CviAII CATG 6 cut(s) 80, 226, 419, 727, 874, 1047
DdeI CTNAG 2 cut(s) 458, 610
DpnI GATC 4 cut(s) 224, 410, 893, 1121
DpnII GATC 4 cut(s) 222, 408, 891, 1119
DraIII CACNNNGTG 2 cut(s) 647, 935
DriI GACNNNNNGTC 1 cut(s) 256
EaeI YGGCCR 2 cut(s) 211, 448
Eam1105I GACNNNNNGTC 1 cut(s) 256
EciI GGCGGA 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 873
Eco32I GATATC 1 cut(s) 588
Eco47I GGWCC 5 cut(s) 203, 440, 723, 771, 851
Eco91I GGTNACC 1 cut(s) 838
EcoO65I GGTNACC 1 cut(s) 838
EcoRII CCWGG 3 cut(s) 436, 938, 1018
EcoRV GATATC 1 cut(s) 588
EcoT14I CCWWGG 1 cut(s) 873
ErhI CCWWGG 1 cut(s) 873
FaeI CATG 6 cut(s) 83, 229, 422, 730, 877, 1050
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FaqI GGGAC 2 cut(s) 263, 269
FatI CATG 6 cut(s) 79, 225, 418, 726, 873, 1046
FbaI TGATCA 1 cut(s) 222
FblI GTMKAC 2 cut(s) 231, 897
Fnu4HI GCNGC 7 cut(s) 102, 130, 172, 211, 455, 746, 780
FokI GGATG 7 cut(s) 299, 342, 568, 576, 815, 956, 1065
Fsp4HI GCNGC 7 cut(s) 102, 130, 172, 211, 455, 746, 780
FspBI CTAG 1 cut(s) 122
GluI GCNGC 7 cut(s) 102, 130, 172, 211, 455, 746, 780
GsaI CCCAGC 2 cut(s) 210, 1015
HaeIII GGCC 4 cut(s) 213, 450, 872, 1018
HgaI GACGC 1 cut(s) 669
Hin1I GRCGYC 1 cut(s) 661
Hin1II CATG 6 cut(s) 83, 229, 422, 730, 877, 1050
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HphI GGTGA 3 cut(s) 141, 301, 418
Hpy166II GTNNAC 5 cut(s) 232, 238, 292, 898, 1003
Hpy188I TCNGA 3 cut(s) 138, 461, 585
Hpy188III TCNNGA 2 cut(s) 526, 826
Hpy8I GTNNAC 5 cut(s) 232, 238, 292, 898, 1003
Hpy99I CGWCG 4 cut(s) 236, 364, 406, 656
HpyCH4III ACNGT 6 cut(s) 301, 406, 425, 487, 740, 935
HpyCH4IV ACGT 6 cut(s) 117, 234, 377, 654, 861, 921
HpyCH4V TGCA 8 cut(s) 51, 83, 281, 536, 559, 748, 779, 929
HpyF10VI GCNNNNNNNGC 6 cut(s) 216, 278, 556, 565, 935, 1042
HpyF3I CTNAG 2 cut(s) 458, 610
HpySE526I ACGT 6 cut(s) 117, 234, 377, 654, 861, 921
Hsp92I GRCGYC 1 cut(s) 661
Hsp92II CATG 6 cut(s) 83, 229, 422, 730, 877, 1050
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
Ksp22I TGATCA 1 cut(s) 222
Kzo9I GATC 4 cut(s) 222, 408, 891, 1119
LmnI GCTCC 4 cut(s) 248, 386, 1022, 1057
Lsp1109I GCAGC 6 cut(s) 113, 141, 183, 466, 732, 791
LweI GCATC 4 cut(s) 388, 537, 546, 1123
MaeI CTAG 1 cut(s) 122
MaeII ACGT 6 cut(s) 117, 234, 377, 654, 861, 921
MaeIII GTNAC 8 cut(s) 94, 147, 332, 431, 481, 647, 680, 838
MalI GATC 4 cut(s) 224, 410, 893, 1121
MboI GATC 4 cut(s) 222, 408, 891, 1119
MfeI CAATTG 1 cut(s) 90
MhlI GDGCHC 2 cut(s) 634, 1054
MluCI AATT 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 181
MnlI CCTC 4 cut(s) 154, 682, 1031, 1097
MroNI GCCGGC 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 475
MseI TTAA 1 cut(s) 68
MslI CAYNNNNRTG 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 210
MunI CAATTG 1 cut(s) 90
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 3 cut(s) 438, 940, 1020
MwoI GCNNNNNNNGC 6 cut(s) 216, 278, 556, 565, 935, 1042
NaeI GCCGGC 1 cut(s) 357
NciI CCSGG 8 cut(s) 247, 287, 327, 391, 552, 836, 889, 1055
NcoI CCATGG 1 cut(s) 873
NdeII GATC 4 cut(s) 222, 408, 891, 1119
NgoMIV GCCGGC 1 cut(s) 355
NlaIII CATG 6 cut(s) 83, 229, 422, 730, 877, 1050
NmuCI GTSAC 4 cut(s) 94, 147, 431, 647
PctI GAATGC 1 cut(s) 10
PdiI GCCGGC 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 475
PflFI GACNNNGTC 1 cut(s) 145
PfoI TCCNGGA 1 cut(s) 887
PinAI ACCGGT 1 cut(s) 842
PkrI GCNGC 7 cut(s) 103, 131, 173, 212, 456, 747, 781
Ppu21I YACGTR 2 cut(s) 118, 862
Psp6I CCWGG 3 cut(s) 436, 938, 1018
PspEI GGTNACC 1 cut(s) 838
PspFI CCCAGC 2 cut(s) 206, 1011
PspGI CCWGG 3 cut(s) 436, 938, 1018
PspPI GGNCC 7 cut(s) 203, 440, 723, 771, 851, 870, 1016
PstNI CAGNNNCTG 1 cut(s) 1116
PsyI GACNNNGTC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 423
SalI GTCGAC 1 cut(s) 230
SaqAI TTAA 1 cut(s) 68
SatI GCNGC 7 cut(s) 102, 130, 172, 211, 455, 746, 780
Sau3AI GATC 4 cut(s) 222, 408, 891, 1119
Sau96I GGNCC 7 cut(s) 203, 440, 723, 771, 851, 870, 1016
ScaI AGTACT 2 cut(s) 884, 970
SduI GDGCHC 2 cut(s) 634, 1054
SfaNI GCATC 4 cut(s) 388, 537, 546, 1123
SfcI CTRYAG 1 cut(s) 787
SinI GGWCC 5 cut(s) 203, 440, 723, 771, 851
SmiMI CAYNNNNRTG 1 cut(s) 423
SmlI CTYRAG 1 cut(s) 1061
SmoI CTYRAG 1 cut(s) 1061
SpeI ACTAGT 1 cut(s) 121
Sse9I AATT 1 cut(s) 90
SsiI CCGC 5 cut(s) 127, 210, 217, 270, 572
SspI AATATT 2 cut(s) 307, 517
SspMI CTAG 1 cut(s) 122
StyI CCWWGG 1 cut(s) 873
TaaI ACNGT 6 cut(s) 301, 406, 425, 487, 740, 935
TaiI ACGT 6 cut(s) 120, 237, 380, 657, 864, 924
TaqI TCGA 3 cut(s) 231, 411, 752
TaqII GACCGA 2 cut(s) 179, 711
TasI AATT 1 cut(s) 90
TatI WGTACW 4 cut(s) 729, 882, 968, 1002
TauI GCSGC 1 cut(s) 213
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 3 cut(s) 90, 436, 1121
TseFI GTSAC 4 cut(s) 94, 147, 431, 647
TseI GCWGC 6 cut(s) 101, 129, 171, 454, 745, 779
Tsp45I GTSAC 4 cut(s) 94, 147, 431, 647
TspDTI ATGAA 3 cut(s) 19, 68, 694
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 3 cut(s) 90, 436, 1121
Tth111I GACNNNGTC 1 cut(s) 145
VpaK11BI GGWCC 5 cut(s) 203, 440, 723, 771, 851
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmiI GTMKAC 2 cut(s) 231, 897
XmnI GAANNNNTTC 1 cut(s) 475
XspI CTAG 1 cut(s) 122
ZrmI AGTACT 2 cut(s) 884, 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.