Rroxscaffold_3G00253900

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012356
Physical Location & Seq
Reverse (-)
48101039 .. 48103251
2213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00253900.1

Sequence Viewer

Length: 1125 bp
ATGGCTTCTTTTCTATCATCTTCATTCTTTTATTTCTACTTCTACTTGGTTTGTTTCGTACTCACTTTGATGTTGTTTCATGCAGAACCAGTTCAAGGTCGAAGAAGCAGCTTTGGTATTCTCAGTTCATCTCCAGCCACCGGAAATAGTAATATTAGGAGCAGCTCATCCAACCATACCAGCAACCAGCGGAAGTGGGTCGGGCCGGTCGGCCACCGCCTCATTACCGTGGACCTAAGAGGTACCGGCGACTTCAAGTCCGTCCAGTCAGCCGTCAATGCAGTTCCGGTCAACAATACGGTCAATGTCCTCATCCACATCAAGCCCGGATGTTACATAGAGAAAGTGCTGGTGCCGGCGACGAAGCCGTACATAACGTTTCAAGGCGCTGGGAAAGATGCGACGGTGATCGAATGGCACGACCGAGCCAGTGACCGTGGACCTAACGGGCAGCAGCTGAGGACTTACAGAACTGCTTCTGTCACGGTCTTGGCCAACTATTTCTCAGCCAGAAACATTAGCTTCAAGAATACGGCACCGGCTCCAATGCCGGGGATGCAAGGATGGCAGGCGGTAGCATTTCGGATATCGGGCGACAAGGCATACTTCTCCGGATGTGGGTTCCATGGCGCCCAAGACACGCTCTGCGACGATGCCGGACGCCATTACTTCAAAGAGTGTTACATCGAGGGTTCCATAGACTTCATCTTTGGAAATGGCCGGTCCATGTACAAAGACTGTGAGTTGCACTCAATAGCTACCAAGTTCGGCTCCATTGCGGCTCAGTTCAGAAAATCACCGGACGATAAATCGGGCTTCGCTTTTCTCAACTGCCGGGTAACGGGTTCGGGTCCACTCTATGTGGGTCGGGCCATGGGCAAGTACGCCCGGATTGTATACTCTTACACATACTTTGATAATGTGGTCGCACATGGCGCCTGGGATGACTGGAACACAACGGGTACAAAGACTGATTTTCTAGGAGTGTACAAGTGCTGGGGTCCTGGAGCAGAAGCCTTGCGAGGACTGTCATATGCACCGGAGCTTGATTTCGACTTGGCGCACCCATTTCTGAGGAAGAGCTTTGTCAATGGAAGACACTGGATAGCCCCATCTGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

374

Amino Acids

41.34

Weight (kDa)

9.15

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 75 - 344 3.4e-55 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47500
fragaria_vesca FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880
malus_domestica MD06G1058600.v1.1
prunus_persica Prupe.2G003200_v2.0.a1 Prupe.5G069600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0203261
rosa_laevigata RLG00000003539
rosa_multiflora Rmu_sc0020346.1_g000001
rosa_roxburghii Rroxscaffold_3G00253900
rosa_rugosa Rorug07G0075400
rosa_samantha Rh7AG204900 Rh7BG204300 Rh7CG215900 Rh7DG211500
rosa_wichuraiana Rw7G017840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 5 cut(s) 242, 352, 535, 629, 935
AccI GTMKAC 1 cut(s) 897
AccIII TCCGGA 1 cut(s) 611
AciI CCGC 4 cut(s) 190, 217, 572, 779
AclI AACGTT 1 cut(s) 377
AcoI YGGCCR 3 cut(s) 211, 492, 718
AcyI GRCGYC 3 cut(s) 630, 661, 936
AfaI GTAC 7 cut(s) 60, 244, 371, 731, 884, 964, 989
AfiI CCNNNNNNNGG 5 cut(s) 95, 140, 551, 618, 841
AgsI TTSAA 5 cut(s) 95, 256, 383, 526, 673
AhdI GACNNNNNGTC 1 cut(s) 256
AjnI CCWGG 2 cut(s) 938, 1003
AluBI AGCT 7 cut(s) 111, 165, 457, 522, 758, 1045, 1083
AluI AGCT 7 cut(s) 111, 165, 457, 522, 758, 1045, 1083
AlwNI CAGNNNCTG 1 cut(s) 457
Aor13HI TCCGGA 1 cut(s) 611
AoxI GGCC 5 cut(s) 203, 211, 492, 718, 870
ApeKI GCWGC 4 cut(s) 108, 162, 451, 454
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
Asp700I GAANNNNTTC 2 cut(s) 90, 475
Asp718I GGTACC 1 cut(s) 242
AspLEI GCGC 4 cut(s) 389, 632, 938, 1063
AspS9I GGNCC 7 cut(s) 203, 232, 440, 723, 851, 870, 1001
AsuC2I CCSGG 4 cut(s) 327, 552, 836, 889
AsuHPI GGTGA 2 cut(s) 418, 789
AvaII GGWCC 5 cut(s) 232, 440, 723, 851, 1001
BalI TGGCCA 1 cut(s) 494
BanI GGYRCC 5 cut(s) 242, 352, 535, 629, 935
BbsI GAAGAC 1 cut(s) 1102
BbvCI CCTCAGC 1 cut(s) 458
BbvI GCAGC 4 cut(s) 120, 174, 463, 466
BccI CCATC 2 cut(s) 558, 1120
BceAI ACGGC 3 cut(s) 257, 352, 549
BciT130I CCWGG 2 cut(s) 940, 1005
BcnI CCSGG 4 cut(s) 327, 552, 836, 889
BfaI CTAG 1 cut(s) 980
BfoI RGCGCY 3 cut(s) 390, 633, 939
BisI GCNGC 5 cut(s) 109, 163, 452, 455, 780
BlsI GCNGC 5 cut(s) 110, 164, 453, 456, 781
Bme1390I CCNGG 6 cut(s) 327, 552, 836, 889, 940, 1005
Bme18I GGWCC 5 cut(s) 232, 440, 723, 851, 1001
BmeRI GACNNNNNGTC 1 cut(s) 256
BmgT120I GGNCC 7 cut(s) 203, 232, 440, 723, 851, 870, 1001
BmrFI CCNGG 6 cut(s) 327, 552, 836, 889, 940, 1005
BmsI GCATC 4 cut(s) 388, 546, 643, 1108
BpiI GAAGAC 1 cut(s) 1102
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 2 cut(s) 117, 1026
Bpu10I CCTNAGC 1 cut(s) 458
BpuMI CCSGG 4 cut(s) 327, 552, 836, 889
BsaHI GRCGYC 3 cut(s) 630, 661, 936
BsaJI CCNNGG 6 cut(s) 228, 436, 551, 625, 873, 939
BsaWI WCCGGW 5 cut(s) 140, 286, 611, 799, 1039
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 140, 551, 618, 841
Bse118I RCCGGY 5 cut(s) 205, 245, 355, 538, 720
Bse1I ACTGG 5 cut(s) 89, 265, 429, 953, 1106
Bse3DI GCAATG 1 cut(s) 774
BseAI TCCGGA 1 cut(s) 611
BseBI CCWGG 2 cut(s) 940, 1005
BseDI CCNNGG 6 cut(s) 228, 436, 551, 625, 873, 939
BseGI GGATG 7 cut(s) 167, 312, 335, 561, 569, 620, 949
BseLI CCNNNNNNNGG 5 cut(s) 95, 140, 551, 618, 841
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 5 cut(s) 136, 449, 519, 797, 1064
BseNI ACTGG 5 cut(s) 89, 265, 429, 953, 1106
BseXI GCAGC 4 cut(s) 120, 174, 463, 466
BseYI CCCAGC 2 cut(s) 389, 996
Bsh1285I CGRYCG 2 cut(s) 210, 424
BshFI GGCC 5 cut(s) 205, 213, 494, 720, 872
BshNI GGYRCC 5 cut(s) 242, 352, 535, 629, 935
BsiEI CGRYCG 2 cut(s) 210, 424
BslI CCNNNNNNNGG 5 cut(s) 95, 140, 551, 618, 841
BsnI GGCC 5 cut(s) 205, 213, 494, 720, 872
Bsp13I TCCGGA 1 cut(s) 611
Bsp1407I TGTACA 2 cut(s) 729, 987
Bsp143I GATC 1 cut(s) 408
Bsp19I CCATGG 2 cut(s) 625, 873
BspACI CCGC 4 cut(s) 190, 217, 572, 779
BspANI GGCC 5 cut(s) 205, 213, 494, 720, 872
BspCNI CTCAG 5 cut(s) 135, 450, 518, 796, 1065
BspEI TCCGGA 1 cut(s) 611
BspQI GCTCTTC 1 cut(s) 1073
BspT107I GGYRCC 5 cut(s) 242, 352, 535, 629, 935
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 5 cut(s) 205, 245, 355, 538, 720
BsrGI TGTACA 2 cut(s) 729, 987
BsrI ACTGG 5 cut(s) 89, 265, 429, 953, 1106
BssAI RCCGGY 5 cut(s) 205, 245, 355, 538, 720
BssECI CCNNGG 6 cut(s) 228, 436, 551, 625, 873, 939
BssMI GATC 1 cut(s) 408
BssNAI GTATAC 1 cut(s) 898
BssNI GRCGYC 3 cut(s) 630, 661, 936
BssT1I CCWWGG 2 cut(s) 625, 873
Bst1107I GTATAC 1 cut(s) 898
Bst2UI CCWGG 2 cut(s) 940, 1005
Bst4CI ACNGT 7 cut(s) 229, 301, 406, 437, 487, 740, 1029
Bst6I CTCTTC 1 cut(s) 1073
BstACI GRCGYC 3 cut(s) 630, 661, 936
BstAUI TGTACA 2 cut(s) 729, 987
BstC8I GCNNGC 2 cut(s) 357, 570
BstDEI CTNAG 6 cut(s) 122, 236, 458, 505, 783, 1073
BstDSI CCRYGG 4 cut(s) 228, 436, 625, 873
BstF5I GGATG 7 cut(s) 167, 312, 335, 561, 569, 620, 949
BstH2I RGCGCY 3 cut(s) 390, 633, 939
BstHHI GCGC 4 cut(s) 389, 632, 938, 1063
BstKTI GATC 1 cut(s) 411
BstMBI GATC 1 cut(s) 408
BstMCI CGRYCG 2 cut(s) 210, 424
BstMWI GCNNNNNNNGC 4 cut(s) 278, 556, 565, 935
BstNI CCWGG 2 cut(s) 940, 1005
BstSCI CCNGG 6 cut(s) 325, 550, 834, 887, 938, 1003
BstV1I GCAGC 4 cut(s) 120, 174, 463, 466
BstV2I GAAGAC 1 cut(s) 1102
BstZ17I GTATAC 1 cut(s) 898
BsuRI GGCC 5 cut(s) 205, 213, 494, 720, 872
BtgI CCRYGG 4 cut(s) 228, 436, 625, 873
BtsCI GGATG 7 cut(s) 167, 312, 335, 561, 569, 620, 949
BtsIMutI CAGTG 2 cut(s) 436, 1099
Cac8I GCNNGC 2 cut(s) 357, 570
CaiI CAGNNNCTG 1 cut(s) 457
CfoI GCGC 4 cut(s) 389, 632, 938, 1063
Cfr10I RCCGGY 5 cut(s) 205, 245, 355, 538, 720
Cfr13I GGNCC 7 cut(s) 203, 232, 440, 723, 851, 870, 1001
CseI GACGC 1 cut(s) 669
Csp6I GTAC 7 cut(s) 59, 243, 370, 730, 883, 963, 988
CviAII CATG 5 cut(s) 80, 626, 727, 874, 932
CviQI GTAC 7 cut(s) 59, 243, 370, 730, 883, 963, 988
DdeI CTNAG 6 cut(s) 122, 236, 458, 505, 783, 1073
DinI GGCGCC 2 cut(s) 631, 937
DpnI GATC 1 cut(s) 410
DpnII GATC 1 cut(s) 408
DriI GACNNNNNGTC 1 cut(s) 256
EaeI YGGCCR 3 cut(s) 211, 492, 718
Eam1104I CTCTTC 1 cut(s) 1073
Eam1105I GACNNNNNGTC 1 cut(s) 256
EarI CTCTTC 1 cut(s) 1073
Eco130I CCWWGG 2 cut(s) 625, 873
Eco32I GATATC 1 cut(s) 588
Eco47I GGWCC 5 cut(s) 232, 440, 723, 851, 1001
EcoO109I RGGNCCY 1 cut(s) 1001
EcoRII CCWGG 2 cut(s) 938, 1003
EcoRV GATATC 1 cut(s) 588
EcoT14I CCWWGG 2 cut(s) 625, 873
EgeI GGCGCC 2 cut(s) 631, 937
EheI GGCGCC 2 cut(s) 631, 937
ErhI CCWWGG 2 cut(s) 625, 873
FaeI CATG 5 cut(s) 83, 629, 730, 877, 935
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FatI CATG 5 cut(s) 79, 625, 726, 873, 931
FauNDI CATATG 1 cut(s) 1033
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 5 cut(s) 109, 163, 452, 455, 780
FokI GGATG 7 cut(s) 154, 299, 342, 568, 576, 627, 956
Fsp4HI GCNGC 5 cut(s) 109, 163, 452, 455, 780
FspBI CTAG 1 cut(s) 980
GlaI GCGC 4 cut(s) 388, 631, 937, 1062
GluI GCNGC 5 cut(s) 109, 163, 452, 455, 780
GsaI CCCAGC 2 cut(s) 393, 1000
GsuI CTGGAG 2 cut(s) 117, 1026
HaeII RGCGCY 3 cut(s) 390, 633, 939
HaeIII GGCC 5 cut(s) 205, 213, 494, 720, 872
HgaI GACGC 1 cut(s) 669
HhaI GCGC 4 cut(s) 389, 632, 938, 1063
Hin1I GRCGYC 3 cut(s) 630, 661, 936
Hin1II CATG 5 cut(s) 83, 629, 730, 877, 935
Hin6I GCGC 4 cut(s) 387, 630, 936, 1061
HinP1I GCGC 4 cut(s) 387, 630, 936, 1061
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HphI GGTGA 2 cut(s) 418, 789
Hpy166II GTNNAC 6 cut(s) 232, 292, 440, 854, 898, 988
Hpy188I TCNGA 4 cut(s) 585, 791, 1074, 1117
Hpy188III TCNNGA 2 cut(s) 526, 612
Hpy8I GTNNAC 6 cut(s) 232, 292, 440, 854, 898, 988
Hpy99I CGWCG 3 cut(s) 364, 406, 653
HpyCH4III ACNGT 7 cut(s) 229, 301, 406, 437, 487, 740, 1029
HpyCH4IV ACGT 1 cut(s) 377
HpyCH4V TGCA 5 cut(s) 83, 281, 559, 748, 1037
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 556, 565, 935
HpyF3I CTNAG 6 cut(s) 122, 236, 458, 505, 783, 1073
HpySE526I ACGT 1 cut(s) 377
Hsp92I GRCGYC 3 cut(s) 630, 661, 936
Hsp92II CATG 5 cut(s) 83, 629, 730, 877, 935
HspAI GCGC 4 cut(s) 387, 630, 936, 1061
KasI GGCGCC 2 cut(s) 629, 935
Kpn2I TCCGGA 1 cut(s) 611
KpnI GGTACC 1 cut(s) 246
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
Kzo9I GATC 1 cut(s) 408
LguI GCTCTTC 1 cut(s) 1073
LmnI GCTCC 5 cut(s) 159, 547, 776, 1007, 1042
Lsp1109I GCAGC 4 cut(s) 120, 174, 463, 466
LweI GCATC 4 cut(s) 388, 546, 643, 1108
MaeI CTAG 1 cut(s) 980
MaeII ACGT 1 cut(s) 377
MaeIII GTNAC 5 cut(s) 332, 431, 481, 680, 838
MalI GATC 1 cut(s) 410
MboI GATC 1 cut(s) 408
MboII GAAGA 4 cut(s) 12, 114, 1090, 1107
MlsI TGGCCA 1 cut(s) 494
MluNI TGGCCA 1 cut(s) 494
Mly113I GGCGCC 2 cut(s) 630, 936
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 7 cut(s) 230, 233, 320, 453, 682, 1016, 1068
Mox20I TGGCCA 1 cut(s) 494
MroI TCCGGA 1 cut(s) 611
MroNI GCCGGC 1 cut(s) 355
MroXI GAANNNNTTC 2 cut(s) 90, 475
MscI TGGCCA 1 cut(s) 494
MseI TTAA 1 cut(s) 1123
MslI CAYNNNNRTG 2 cut(s) 68, 227
Msp20I TGGCCA 1 cut(s) 494
MspA1I CMGCKG 2 cut(s) 190, 457
MspR9I CCNGG 6 cut(s) 327, 552, 836, 889, 940, 1005
MvaI CCWGG 2 cut(s) 940, 1005
MwoI GCNNNNNNNGC 4 cut(s) 278, 556, 565, 935
NaeI GCCGGC 1 cut(s) 357
NarI GGCGCC 2 cut(s) 630, 936
NciI CCSGG 4 cut(s) 327, 552, 836, 889
NcoI CCATGG 2 cut(s) 625, 873
NdeI CATATG 1 cut(s) 1033
NdeII GATC 1 cut(s) 408
NgoMIV GCCGGC 1 cut(s) 355
NlaIII CATG 5 cut(s) 83, 629, 730, 877, 935
NmuCI GTSAC 2 cut(s) 431, 481
PciSI GCTCTTC 1 cut(s) 1073
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PdiI GCCGGC 1 cut(s) 357
PdmI GAANNNNTTC 2 cut(s) 90, 475
PfoI TCCNGGA 1 cut(s) 1003
PkrI GCNGC 5 cut(s) 110, 164, 453, 456, 781
PluTI GGCGCC 2 cut(s) 633, 939
PpuMI RGGWCCY 1 cut(s) 1001
Psp1406I AACGTT 1 cut(s) 377
Psp5II RGGWCCY 1 cut(s) 1001
Psp6I CCWGG 2 cut(s) 938, 1003
PspFI CCCAGC 2 cut(s) 389, 996
PspGI CCWGG 2 cut(s) 938, 1003
PspPI GGNCC 7 cut(s) 203, 232, 440, 723, 851, 870, 1001
PspPPI RGGWCCY 1 cut(s) 1001
PsrI GAACNNNNNNTAC 2 cut(s) 109, 141
PstNI CAGNNNCTG 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 457
RsaI GTAC 7 cut(s) 60, 244, 371, 731, 884, 964, 989
RsaNI GTAC 7 cut(s) 59, 243, 370, 730, 883, 963, 988
RseI CAYNNNNRTG 2 cut(s) 68, 227
SapI GCTCTTC 1 cut(s) 1073
SaqAI TTAA 1 cut(s) 1123
SatI GCNGC 5 cut(s) 109, 163, 452, 455, 780
Sau3AI GATC 1 cut(s) 408
Sau96I GGNCC 7 cut(s) 203, 232, 440, 723, 851, 870, 1001
ScrFI CCNGG 6 cut(s) 327, 552, 836, 889, 940, 1005
SfaNI GCATC 4 cut(s) 388, 546, 643, 1108
SfoI GGCGCC 2 cut(s) 631, 937
SinI GGWCC 5 cut(s) 232, 440, 723, 851, 1001
SmiMI CAYNNNNRTG 2 cut(s) 68, 227
SsiI CCGC 4 cut(s) 190, 217, 572, 779
SspDI GGCGCC 2 cut(s) 629, 935
SspI AATATT 1 cut(s) 154
SspMI CTAG 1 cut(s) 980
StyD4I CCNGG 6 cut(s) 325, 550, 834, 887, 938, 1003
StyI CCWWGG 2 cut(s) 625, 873
TaaI ACNGT 7 cut(s) 229, 301, 406, 437, 487, 740, 1029
TaiI ACGT 1 cut(s) 380
TaqI TCGA 4 cut(s) 100, 411, 687, 1053
TaqII GACCGA 1 cut(s) 438
TatI WGTACW 2 cut(s) 729, 987
TauI GCSGC 1 cut(s) 782
Tru1I TTAA 1 cut(s) 1123
Tru9I TTAA 1 cut(s) 1123
TscAI CASTG 2 cut(s) 436, 1106
TseFI GTSAC 2 cut(s) 431, 481
TseI GCWGC 4 cut(s) 108, 162, 451, 454
Tsp45I GTSAC 2 cut(s) 431, 481
TspDTI ATGAA 4 cut(s) 12, 68, 117, 694
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 2 cut(s) 436, 1106
VpaK11BI GGWCC 5 cut(s) 232, 440, 723, 851, 1001
XmiI GTMKAC 1 cut(s) 897
XmnI GAANNNNTTC 2 cut(s) 90, 475
XspI CTAG 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.