Prupe.5G069600_v2.0.a1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0012356
Physical Location & Seq
Forward (+)
8359985 .. 8363270
3286 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G069600.1

Sequence Viewer

Length: 1146 bp
ATGGCTTCTTCTCTTTTATCATCATCATCTTCTTCTTCTTCTTCTTACTTCTTCTTGGTGTGCACCACACTCATTGTGTTTTCCTTCTGGTTATTTCATGCAGCGCCAGCGGTGACAGCCGTGAGAAGCTCTCGCGTAATGCTAGTCCACACTACTAACAACAATGGTTCAACATCAAACTCTACCAAGAACCACCACAAGTGGGTGGGGCCAACTGGTCACCGCCAGATCGCTGTCGACATAAACGGTTCAGGGGATTTCCTGTCCGTCCAAGCCGCCGTCAATGCAGTACCGGCAAACAACACGGTCAATGTTCTCATCCTAATCAGCCCCGGATACTACAGAGAAAAAGTGGTGGTGCCGGCGACAAAGCCGTACATCACGTTTCAAGGAGCTGGGAAAGATGTGACCGTGATCGAATGGCATGACCGAGCCAGTGACCCTGGGCCCAACGGCCAGCAGCTGAGGACTTACAGGACAGCTTCTGTTACTGTTTTCGCCAACTATTTCTCCGCAAGAAACATTAGCTTCAAGAATACGGCGCCGGCTCCGATGCCGGGAATGCAAGGGTGGCAAGCGGTAGCGTTTAGGATATCAGGTGACAAGGCATACTTCTCAGGTTGTGGCTTCTATGGTGCCCAAGACACACTTTGTGACGATGTGGGGCGCCATTACTTCAAGGATTGTTACATTGAGGGTTCCATAGACTTCATTTTTGGGAATGGTCGGTCCATGTACAAAGACTGTGAGCTGCACTCGATAGCAACCAAGTTCGGGTCAATTGCGGCCCACTACAGAAACTCAGCGGATGATAAATCAGGCTTCGCTTTCGTGAACTGCCGAGTTACGGGTACGGGTCCTTTGTATGTGGGTCGGGCCATGGGCAAGTACTCCCGGATCGTATACTCCTACACCTACTTTGATGACGTGGTCGCTCATGGTGCCTGGGATGACTGGGGCAACACAACCGCCAGCACTACAACAAAGAGGAGTGTGTTTATGGGAGTCTACAAGTGCTGGGGGCCAGGAGAAGAAGCCCTGCGTGGGGTGTCATGTGCCCCAGAGCTTGATTATGAATTGGCCCATCCCTTTCTGGTAAAGAGTTTCGTCAACGGAAGGCACTGGATTGCACCCTCTGATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

382

Amino Acids

41.67

Weight (kDa)

8.67

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47500
fragaria_vesca FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880
malus_domestica MD06G1058600.v1.1
prunus_persica Prupe.2G003200_v2.0.a1 Prupe.5G069600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0203261
rosa_laevigata RLG00000003539
rosa_multiflora Rmu_sc0020346.1_g000001
rosa_roxburghii Rroxscaffold_3G00253900
rosa_rugosa Rorug07G0075400
rosa_samantha Rh7AG204900 Rh7BG204300 Rh7CG215900 Rh7DG211500
rosa_wichuraiana Rw7G017840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 358, 541, 635, 666, 941
AccI GTMKAC 3 cut(s) 237, 903, 1008
AccII CGCG 1 cut(s) 135
AciI CCGC 8 cut(s) 110, 223, 276, 513, 578, 785, 806, 969
AclWI GGATC 1 cut(s) 905
AcoI YGGCCR 1 cut(s) 454
AcyI GRCGYC 2 cut(s) 542, 667
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 5 cut(s) 291, 377, 737, 853, 890
AfiI CCNNNNNNNGG 6 cut(s) 202, 557, 774, 847, 1044, 1045
AgsI TTSAA 4 cut(s) 171, 389, 532, 679
AjiI CACGTC 1 cut(s) 928
AjnI CCWGG 3 cut(s) 442, 944, 1024
AluBI AGCT 7 cut(s) 129, 395, 463, 482, 528, 751, 1066
AluI AGCT 7 cut(s) 129, 395, 463, 482, 528, 751, 1066
Alw21I GWGCWC 1 cut(s) 65
Alw44I GTGCAC 1 cut(s) 61
AlwI GGATC 1 cut(s) 905
AlwNI CAGNNNCTG 2 cut(s) 463, 485
AoxI GGCC 7 cut(s) 209, 446, 454, 786, 876, 1022, 1080
ApaI GGGCCC 1 cut(s) 450
ApaLI GTGCAC 1 cut(s) 61
ApeKI GCWGC 3 cut(s) 101, 460, 751
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AspLEI GCGC 3 cut(s) 106, 544, 669
AspS9I GGNCC 9 cut(s) 209, 446, 447, 729, 787, 857, 876, 1022, 1081
AsuC2I CCSGG 3 cut(s) 333, 558, 895
AsuHPI GGTGA 3 cut(s) 124, 212, 611
AvaII GGWCC 2 cut(s) 729, 857
BaeGI GKGCMC 4 cut(s) 65, 450, 640, 1060
BanI GGYRCC 5 cut(s) 358, 541, 635, 666, 941
BanII GRGCYC 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 65
BbvCI CCTCAGC 1 cut(s) 464
BbvI GCAGC 3 cut(s) 113, 472, 738
BccI CCATC 1 cut(s) 1092
BceAI ACGGC 5 cut(s) 104, 263, 358, 469, 555
BciT130I CCWGG 3 cut(s) 444, 946, 1026
BciVI GTATCC 1 cut(s) 329
BcnI CCSGG 3 cut(s) 333, 558, 895
BfaI CTAG 1 cut(s) 143
BfmI CTRYAG 2 cut(s) 340, 793
BfoI RGCGCY 3 cut(s) 107, 545, 670
BfuI GTATCC 1 cut(s) 329
BisI GCNGC 5 cut(s) 102, 276, 461, 752, 786
BlsI GCNGC 5 cut(s) 103, 277, 462, 753, 787
BmcAI AGTACT 1 cut(s) 890
Bme1390I CCNGG 6 cut(s) 333, 444, 558, 895, 946, 1026
Bme18I GGWCC 2 cut(s) 729, 857
BmgBI CACGTC 1 cut(s) 928
BmgT120I GGNCC 9 cut(s) 209, 446, 447, 729, 787, 857, 876, 1022, 1081
BmrFI CCNGG 6 cut(s) 333, 444, 558, 895, 946, 1026
BmrI ACTGGG 1 cut(s) 964
BmsI GCATC 2 cut(s) 543, 1129
BmuI ACTGGG 1 cut(s) 964
BplI GAGNNNNNCTC 4 cut(s) 115, 147, 740, 772
Bpu10I CCTNAGC 1 cut(s) 464
BpuMI CCSGG 3 cut(s) 333, 558, 895
BsaHI GRCGYC 2 cut(s) 542, 667
BsaJI CCNNGG 5 cut(s) 331, 442, 443, 879, 945
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 6 cut(s) 202, 557, 774, 847, 1044, 1045
Bse118I RCCGGY 3 cut(s) 292, 361, 544
Bse1I ACTGG 4 cut(s) 220, 435, 959, 1127
BseBI CCWGG 3 cut(s) 444, 946, 1026
BseDI CCNNGG 5 cut(s) 331, 442, 443, 879, 945
BseGI GGATG 4 cut(s) 318, 814, 955, 1084
BseLI CCNNNNNNNGG 6 cut(s) 202, 557, 774, 847, 1044, 1045
BseMII CTCAG 3 cut(s) 455, 630, 816
BseNI ACTGG 4 cut(s) 220, 435, 959, 1127
BseRI GAGGAG 1 cut(s) 1003
BseSI GKGCMC 4 cut(s) 65, 450, 640, 1060
BseXI GCAGC 3 cut(s) 113, 472, 738
BseYI CCCAGC 2 cut(s) 395, 1017
BsgI GTGCAG 1 cut(s) 737
Bsh1236I CGCG 1 cut(s) 135
BshFI GGCC 7 cut(s) 211, 448, 456, 788, 878, 1024, 1082
BshNI GGYRCC 5 cut(s) 358, 541, 635, 666, 941
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 6 cut(s) 293, 333, 362, 545, 557, 895
BslI CCNNNNNNNGG 6 cut(s) 202, 557, 774, 847, 1044, 1045
BsmI GAATGC 1 cut(s) 567
BsnI GGCC 7 cut(s) 211, 448, 456, 788, 878, 1024, 1082
Bsp120I GGGCCC 1 cut(s) 446
Bsp1286I GDGCHC 4 cut(s) 65, 450, 640, 1060
Bsp1407I TGTACA 1 cut(s) 735
Bsp143I GATC 3 cut(s) 228, 414, 897
Bsp19I CCATGG 1 cut(s) 879
BspACI CCGC 8 cut(s) 110, 223, 276, 513, 578, 785, 806, 969
BspANI GGCC 7 cut(s) 211, 448, 456, 788, 878, 1024, 1082
BspCNI CTCAG 3 cut(s) 456, 629, 815
BspFNI CGCG 1 cut(s) 135
BspPI GGATC 1 cut(s) 905
BspT107I GGYRCC 5 cut(s) 358, 541, 635, 666, 941
BsrFI RCCGGY 3 cut(s) 292, 361, 544
BsrGI TGTACA 1 cut(s) 735
BsrI ACTGG 4 cut(s) 220, 435, 959, 1127
BssAI RCCGGY 3 cut(s) 292, 361, 544
BssECI CCNNGG 5 cut(s) 331, 442, 443, 879, 945
BssMI GATC 3 cut(s) 228, 414, 897
BssNAI GTATAC 1 cut(s) 904
BssNI GRCGYC 2 cut(s) 542, 667
BssT1I CCWWGG 1 cut(s) 879
Bst1107I GTATAC 1 cut(s) 904
Bst2UI CCWGG 3 cut(s) 444, 946, 1026
Bst4CI ACNGT 5 cut(s) 248, 307, 412, 493, 746
BstACI GRCGYC 2 cut(s) 542, 667
BstAUI TGTACA 1 cut(s) 735
BstC8I GCNNGC 6 cut(s) 108, 363, 458, 546, 576, 973
BstDEI CTNAG 4 cut(s) 464, 616, 802, 1143
BstDSI CCRYGG 1 cut(s) 879
BstEII GGTNACC 1 cut(s) 218
BstF5I GGATG 4 cut(s) 318, 814, 955, 1084
BstFNI CGCG 1 cut(s) 135
BstH2I RGCGCY 3 cut(s) 107, 545, 670
BstHHI GCGC 3 cut(s) 106, 544, 669
BstKTI GATC 3 cut(s) 231, 417, 900
BstMBI GATC 3 cut(s) 228, 414, 897
BstMWI GCNNNNNNNGC 7 cut(s) 107, 116, 284, 293, 562, 571, 941
BstNI CCWGG 3 cut(s) 444, 946, 1026
BstPI GGTNACC 1 cut(s) 218
BstSCI CCNGG 6 cut(s) 331, 442, 556, 893, 944, 1024
BstSFI CTRYAG 2 cut(s) 340, 793
BstSLI GKGCMC 4 cut(s) 65, 450, 640, 1060
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 3 cut(s) 113, 472, 738
BstZ17I GTATAC 1 cut(s) 904
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 7 cut(s) 211, 448, 456, 788, 878, 1024, 1082
BtgI CCRYGG 1 cut(s) 879
BtrI CACGTC 1 cut(s) 928
BtsCI GGATG 4 cut(s) 318, 814, 955, 1084
BtsIMutI CAGTG 2 cut(s) 442, 1120
Cac8I GCNNGC 6 cut(s) 108, 363, 458, 546, 576, 973
CaiI CAGNNNCTG 2 cut(s) 463, 485
CfoI GCGC 3 cut(s) 106, 544, 669
Cfr10I RCCGGY 3 cut(s) 292, 361, 544
Cfr13I GGNCC 9 cut(s) 209, 446, 447, 729, 787, 857, 876, 1022, 1081
Csp6I GTAC 5 cut(s) 290, 376, 736, 852, 889
CspCI CAANNNNNGTGG 2 cut(s) 55, 90
CviAII CATG 6 cut(s) 98, 425, 733, 880, 938, 1053
CviQI GTAC 5 cut(s) 290, 376, 736, 852, 889
DdeI CTNAG 4 cut(s) 464, 616, 802, 1143
DinI GGCGCC 2 cut(s) 543, 668
DpnI GATC 3 cut(s) 230, 416, 899
DpnII GATC 3 cut(s) 228, 414, 897
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 879
Eco24I GRGCYC 1 cut(s) 450
Eco32I GATATC 1 cut(s) 594
Eco47I GGWCC 2 cut(s) 729, 857
Eco91I GGTNACC 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 857
EcoO65I GGTNACC 1 cut(s) 218
EcoRII CCWGG 3 cut(s) 442, 944, 1024
EcoRV GATATC 1 cut(s) 594
EcoT14I CCWWGG 1 cut(s) 879
EcoT38I GRGCYC 1 cut(s) 450
EgeI GGCGCC 2 cut(s) 543, 668
EheI GGCGCC 2 cut(s) 543, 668
ErhI CCWWGG 1 cut(s) 879
FaeI CATG 6 cut(s) 101, 428, 736, 883, 941, 1056
FalI AAGNNNNNCTT 4 cut(s) 596, 628, 633, 665
FatI CATG 6 cut(s) 97, 424, 732, 879, 937, 1052
FblI GTMKAC 3 cut(s) 237, 903, 1008
Fnu4HI GCNGC 5 cut(s) 102, 276, 461, 752, 786
FokI GGATG 4 cut(s) 305, 821, 962, 1071
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 5 cut(s) 102, 276, 461, 752, 786
FspBI CTAG 1 cut(s) 143
GlaI GCGC 3 cut(s) 105, 543, 668
GluI GCNGC 5 cut(s) 102, 276, 461, 752, 786
GsaI CCCAGC 2 cut(s) 399, 1021
HaeII RGCGCY 3 cut(s) 107, 545, 670
HaeIII GGCC 7 cut(s) 211, 448, 456, 788, 878, 1024, 1082
HapII CCGG 6 cut(s) 293, 333, 362, 545, 557, 895
HhaI GCGC 3 cut(s) 106, 544, 669
Hin1I GRCGYC 2 cut(s) 542, 667
Hin1II CATG 6 cut(s) 101, 428, 736, 883, 941, 1056
Hin6I GCGC 3 cut(s) 104, 542, 667
HinP1I GCGC 3 cut(s) 104, 542, 667
HincII GTYRAC 2 cut(s) 238, 1111
HindII GTYRAC 2 cut(s) 238, 1111
HinfI GANTC 1 cut(s) 1005
HpaII CCGG 6 cut(s) 293, 333, 362, 545, 557, 895
HphI GGTGA 3 cut(s) 124, 212, 611
Hpy166II GTNNAC 7 cut(s) 63, 148, 238, 835, 904, 1009, 1111
Hpy188I TCNGA 2 cut(s) 552, 1138
Hpy188III TCNNGA 2 cut(s) 532, 832
Hpy8I GTNNAC 7 cut(s) 63, 148, 238, 835, 904, 1009, 1111
HpyAV CCTTC 2 cut(s) 94, 1110
HpyCH4III ACNGT 5 cut(s) 248, 307, 412, 493, 746
HpyCH4IV ACGT 2 cut(s) 383, 927
HpyCH4V TGCA 6 cut(s) 63, 101, 287, 565, 754, 1130
HpyF10VI GCNNNNNNNGC 7 cut(s) 107, 116, 284, 293, 562, 571, 941
HpyF3I CTNAG 4 cut(s) 464, 616, 802, 1143
HpySE526I ACGT 2 cut(s) 383, 927
Hsp92I GRCGYC 2 cut(s) 542, 667
Hsp92II CATG 6 cut(s) 101, 428, 736, 883, 941, 1056
HspAI GCGC 3 cut(s) 104, 542, 667
KasI GGCGCC 2 cut(s) 541, 666
KroI GCCGGC 2 cut(s) 361, 544
KroNI GCCGGC 2 cut(s) 363, 546
Kzo9I GATC 3 cut(s) 228, 414, 897
LmnI GCTCC 2 cut(s) 392, 553
Lsp1109I GCAGC 3 cut(s) 113, 472, 738
LweI GCATC 2 cut(s) 543, 1129
MaeI CTAG 1 cut(s) 143
MaeII ACGT 2 cut(s) 383, 927
MaeIII GTNAC 9 cut(s) 112, 218, 406, 437, 487, 599, 653, 686, 844
MalI GATC 3 cut(s) 230, 416, 899
MboI GATC 3 cut(s) 228, 414, 897
MboII GAAGA 7 cut(s) 21, 24, 27, 30, 33, 43, 1043
MfeI CAATTG 1 cut(s) 780
MhlI GDGCHC 4 cut(s) 65, 450, 640, 1060
MluCI AATT 2 cut(s) 780, 1076
Mly113I GGCGCC 2 cut(s) 542, 667
MlyI GAGTC 1 cut(s) 1014
MnlI CCTC 4 cut(s) 459, 688, 981, 1144
MroNI GCCGGC 2 cut(s) 361, 544
MspA1I CMGCKG 3 cut(s) 110, 463, 806
MspI CCGG 6 cut(s) 293, 333, 362, 545, 557, 895
MspR9I CCNGG 6 cut(s) 333, 444, 558, 895, 946, 1026
MunI CAATTG 1 cut(s) 780
Mva1269I GAATGC 1 cut(s) 567
MvaI CCWGG 3 cut(s) 444, 946, 1026
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 7 cut(s) 107, 116, 284, 293, 562, 571, 941
NaeI GCCGGC 2 cut(s) 363, 546
NarI GGCGCC 2 cut(s) 542, 667
NciI CCSGG 3 cut(s) 333, 558, 895
NcoI CCATGG 1 cut(s) 879
NdeII GATC 3 cut(s) 228, 414, 897
NgoMIV GCCGGC 2 cut(s) 361, 544
NlaIII CATG 6 cut(s) 101, 428, 736, 883, 941, 1056
NmeAIII GCCGAG 1 cut(s) 866
NmuCI GTSAC 6 cut(s) 112, 218, 406, 437, 599, 653
PasI CCCWGGG 1 cut(s) 443
PctI GAATGC 1 cut(s) 567
PdiI GCCGGC 2 cut(s) 363, 546
PflFI GACNNNGTC 1 cut(s) 929
PfoI TCCNGGA 1 cut(s) 893
PkrI GCNGC 5 cut(s) 103, 277, 462, 753, 787
PleI GAGTC 1 cut(s) 1013
PluTI GGCGCC 2 cut(s) 545, 670
PpsI GAGTC 1 cut(s) 1013
PpuMI RGGWCCY 1 cut(s) 857
Psp5II RGGWCCY 1 cut(s) 857
Psp6I CCWGG 3 cut(s) 442, 944, 1024
PspEI GGTNACC 1 cut(s) 218
PspFI CCCAGC 2 cut(s) 395, 1017
PspGI CCWGG 3 cut(s) 442, 944, 1024
PspOMI GGGCCC 1 cut(s) 446
PspPI GGNCC 9 cut(s) 209, 446, 447, 729, 787, 857, 876, 1022, 1081
PspPPI RGGWCCY 1 cut(s) 857
PstNI CAGNNNCTG 2 cut(s) 463, 485
PsyI GACNNNGTC 1 cut(s) 929
PvuII CAGCTG 1 cut(s) 463
RsaI GTAC 5 cut(s) 291, 377, 737, 853, 890
RsaNI GTAC 5 cut(s) 290, 376, 736, 852, 889
SalI GTCGAC 1 cut(s) 236
SatI GCNGC 5 cut(s) 102, 276, 461, 752, 786
Sau3AI GATC 3 cut(s) 228, 414, 897
Sau96I GGNCC 9 cut(s) 209, 446, 447, 729, 787, 857, 876, 1022, 1081
ScaI AGTACT 1 cut(s) 890
SchI GAGTC 1 cut(s) 1014
ScrFI CCNGG 6 cut(s) 333, 444, 558, 895, 946, 1026
SduI GDGCHC 4 cut(s) 65, 450, 640, 1060
SfaNI GCATC 2 cut(s) 543, 1129
SfcI CTRYAG 2 cut(s) 340, 793
SfoI GGCGCC 2 cut(s) 543, 668
SinI GGWCC 2 cut(s) 729, 857
Sse9I AATT 2 cut(s) 780, 1076
SsiI CCGC 8 cut(s) 110, 223, 276, 513, 578, 785, 806, 969
SspDI GGCGCC 2 cut(s) 541, 666
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 6 cut(s) 331, 442, 556, 893, 944, 1024
StyI CCWWGG 1 cut(s) 879
TaaI ACNGT 5 cut(s) 248, 307, 412, 493, 746
TaiI ACGT 2 cut(s) 386, 930
TaqI TCGA 3 cut(s) 237, 417, 758
TaqII GACCGA 2 cut(s) 444, 717
TasI AATT 2 cut(s) 780, 1076
TatI WGTACW 2 cut(s) 735, 888
TauI GCSGC 2 cut(s) 278, 788
TscAI CASTG 2 cut(s) 442, 1127
TseFI GTSAC 6 cut(s) 112, 218, 406, 437, 599, 653
TseI GCWGC 3 cut(s) 101, 460, 751
Tsp45I GTSAC 6 cut(s) 112, 218, 406, 437, 599, 653
TspDTI ATGAA 3 cut(s) 86, 700, 1089
TspGWI ACGGA 2 cut(s) 256, 1128
TspRI CASTG 2 cut(s) 442, 1127
Tth111I GACNNNGTC 1 cut(s) 929
VneI GTGCAC 1 cut(s) 61
VpaK11BI GGWCC 2 cut(s) 729, 857
XmiI GTMKAC 3 cut(s) 237, 903, 1008
XspI CTAG 1 cut(s) 143
ZrmI AGTACT 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.