AT5G49920

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016581
Physical Location & Seq
Reverse (-)
20306409 .. 20307943
1535 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49920.1

Sequence Viewer

Length: 867 bp
ATGGCGGAGGAGAGATCGCCGAGTAAGGTGAAGTTCATGTGCAGTTTTGGCGGCAGAATCCTCCCTAGACCATCGGACAGTGTTCTCAAATATGTCGGCGGAGAGACTCGCGTCGTTGCTGTATCTCCTGACATCTCCTTCTCCGAACTCATGAAGAAACTTACGGCAATCACAGAGAACGATATTGTGCTCAAATACCAGATCATTCCAGAAGATCTTGATGCATTAGTTTCTGTAAAGTCTGATGAAGATGTGAAACACATGATAGAGGAATATAACCGTCATGAAACACCCAAGCTCAGAACTTTCTTGTTTCCTGCAAACCCAGTGGTTCTTGAGAGTCAGTTAGGCCCCATCGAGCCACAAACCATAGAGCAACGCTACATTGAAGCCATCAATGGCATTCTTCGCACATCCAAGAGTGCTACTGCTCTTCGTGCTCCCATCAAAACACGACCTTCCTTCACCGTCTCTACTTGCTCCTCTCCCAAATCAGAATCACCTGATGGATACAGTCACGAGCCGCCAGAAACGAGTTTTCAGAACAACTACCAATTGAGCCGGCTTTACCCAATGCATAGAGTGCATAGCAGTCCCAACATCTCACACCAGCTGCAGCCACACAGCCACTACCACAACCACAACCACAATGCATACGTGCAGCCTCCTACTAACTACTTAACGTGTAGGCCACGACTACCAACACCGTTAGAAATTCCCAGAGGCATCGGTTGGGACCAATCATACACAACCACACATAACTCAGGTGGCGGTAATGGCAAGTACGGGTGCAATGATGACCGCAGGTTCTGGGGAAGAGCAAGCAGCGTTCCTCAAAGTCCTAGGAACCATGGACTCCGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

288

Amino Acids

32.49

Weight (kDa)

8.8

Isoelectric Point (pI)

67.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 29 - 106 4.7e-15 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016581)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49920
fragaria_vesca FvH4_5g28590
malus_domestica MD15G1436100.v1.1
prunus_persica Prupe.1G579900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220591
rosa_laevigata RLG00000002215
rosa_multiflora Rmu_ssc0000189.1_g000028
rosa_roxburghii Rroxscaffold_3G00239280
rosa_rugosa Rorug07G0195200
rosa_samantha Rh7AG336200 Rh7BG327300 Rh7CG353900 Rh7DG332500
rosa_wichuraiana Rw7G028680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 795
AccII CGCG 1 cut(s) 111
AciI CCGC 7 cut(s) 5, 51, 99, 524, 771, 802, 859
AcsI RAATTY 1 cut(s) 714
AfaI GTAC 1 cut(s) 785
AflIII ACRYGT 1 cut(s) 683
AgsI TTSAA 1 cut(s) 389
AluBI AGCT 2 cut(s) 298, 613
AluI AGCT 2 cut(s) 298, 613
Alw21I GWGCWC 2 cut(s) 192, 442
Alw26I GTCTC 2 cut(s) 98, 475
AlwNI CAGNNNCTG 1 cut(s) 810
AoxI GGCC 2 cut(s) 349, 689
ApeKI GCWGC 4 cut(s) 613, 616, 661, 825
ApoI RAATTY 1 cut(s) 714
AspA2I CCTAGG 1 cut(s) 842
AspS9I GGNCC 2 cut(s) 350, 736
AsuHPI GGTGA 3 cut(s) 40, 457, 492
AvaII GGWCC 1 cut(s) 736
AvrII CCTAGG 1 cut(s) 842
BauI CACGAG 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 192, 442
BbvI GCAGC 4 cut(s) 600, 628, 673, 837
BccI CCATC 5 cut(s) 79, 362, 401, 452, 500
BceAI ACGGC 1 cut(s) 180
BciVI GTATCC 1 cut(s) 503
BcoDI GTCTC 2 cut(s) 98, 475
BfaI CTAG 2 cut(s) 66, 843
BfmI CTRYAG 1 cut(s) 614
BfuAI ACCTGC 1 cut(s) 795
BfuI GTATCC 1 cut(s) 503
BglII AGATCT 1 cut(s) 214
BisI GCNGC 6 cut(s) 52, 524, 614, 617, 662, 826
BlnI CCTAGG 1 cut(s) 842
BlsI GCNGC 6 cut(s) 53, 525, 615, 618, 663, 827
Bme18I GGWCC 1 cut(s) 736
BmgT120I GGNCC 2 cut(s) 350, 736
BmiI GGNNCC 3 cut(s) 352, 737, 848
BmrI ACTGGG 1 cut(s) 320
BmsI GCATC 2 cut(s) 211, 735
BmuI ACTGGG 1 cut(s) 320
BoxI GACNNNNGTC 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 658
BsaJI CCNNGG 2 cut(s) 842, 850
Bse118I RCCGGY 1 cut(s) 561
Bse1I ACTGG 1 cut(s) 326
Bse3DI GCAATG 1 cut(s) 799
BseDI CCNNGG 2 cut(s) 842, 850
BseGI GGATG 1 cut(s) 413
BseMI GCAATG 1 cut(s) 799
BseMII CTCAG 2 cut(s) 313, 777
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 2 cut(s) 23, 472
BseXI GCAGC 4 cut(s) 600, 628, 673, 837
BsgI GTGCAG 2 cut(s) 61, 680
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 351, 691
BsiHKAI GWGCWC 2 cut(s) 192, 442
BsiSI CCGG 1 cut(s) 562
BslFI GGGAC 2 cut(s) 579, 749
BsmAI GTCTC 2 cut(s) 98, 475
BsmBI CGTCTC 1 cut(s) 475
BsmFI GGGAC 2 cut(s) 579, 749
BsmI GAATGC 1 cut(s) 402
BsnI GGCC 2 cut(s) 351, 691
Bsp1286I GDGCHC 2 cut(s) 192, 442
Bsp143I GATC 3 cut(s) 14, 201, 214
Bsp19I CCATGG 1 cut(s) 850
BspACI CCGC 7 cut(s) 5, 51, 99, 524, 771, 802, 859
BspANI GGCC 2 cut(s) 351, 691
BspCNI CTCAG 2 cut(s) 312, 776
BspFNI CGCG 1 cut(s) 111
BspHI TCATGA 2 cut(s) 150, 283
BspLI GGNNCC 3 cut(s) 352, 737, 848
BspMAI CTGCAG 1 cut(s) 618
BspMI ACCTGC 1 cut(s) 795
BspQI GCTCTTC 2 cut(s) 438, 811
BsrDI GCAATG 1 cut(s) 799
BsrFI RCCGGY 1 cut(s) 561
BsrI ACTGG 1 cut(s) 326
BssAI RCCGGY 1 cut(s) 561
BssECI CCNNGG 2 cut(s) 842, 850
BssMI GATC 3 cut(s) 14, 201, 214
BssSI CACGAG 1 cut(s) 518
BssT1I CCWWGG 2 cut(s) 842, 850
Bst2BI CACGAG 1 cut(s) 518
Bst4CI ACNGT 5 cut(s) 80, 281, 469, 515, 708
Bst6I CTCTTC 2 cut(s) 438, 811
BstAPI GCANNNNNTGC 1 cut(s) 583
BstBAI YACGTR 1 cut(s) 658
BstC8I GCNNGC 2 cut(s) 563, 823
BstDEI CTNAG 2 cut(s) 299, 763
BstDSI CCRYGG 1 cut(s) 850
BstF5I GGATG 1 cut(s) 413
BstFNI CGCG 1 cut(s) 111
BstKTI GATC 3 cut(s) 17, 204, 217
BstMAI GTCTC 2 cut(s) 98, 475
BstMBI GATC 3 cut(s) 14, 201, 214
BstMWI GCNNNNNNNGC 5 cut(s) 48, 408, 437, 583, 777
BstPAI GACNNNNGTC 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 614
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 4 cut(s) 600, 628, 673, 837
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuI GTATCC 1 cut(s) 503
BsuRI GGCC 2 cut(s) 351, 691
BtgI CCRYGG 1 cut(s) 850
BtsCI GGATG 1 cut(s) 413
BtsIMutI CAGTG 2 cut(s) 85, 333
BveI ACCTGC 1 cut(s) 795
Cac8I GCNNGC 2 cut(s) 563, 823
CaiI CAGNNNCTG 1 cut(s) 810
CciI TCATGA 2 cut(s) 150, 283
Cfr10I RCCGGY 1 cut(s) 561
Cfr13I GGNCC 2 cut(s) 350, 736
CseI GACGC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 784
CspCI CAANNNNNGTGG 2 cut(s) 309, 344
CviAII CATG 5 cut(s) 37, 151, 262, 284, 851
CviQI GTAC 1 cut(s) 784
DdeI CTNAG 2 cut(s) 299, 763
DpnI GATC 3 cut(s) 16, 203, 216
DpnII GATC 3 cut(s) 14, 201, 214
Eam1104I CTCTTC 2 cut(s) 438, 811
EarI CTCTTC 2 cut(s) 438, 811
EciI GGCGGA 3 cut(s) 20, 114, 848
Eco130I CCWWGG 2 cut(s) 842, 850
Eco47I GGWCC 1 cut(s) 736
EcoO109I RGGNCCY 1 cut(s) 350
EcoT14I CCWWGG 2 cut(s) 842, 850
EcoT22I ATGCAT 3 cut(s) 226, 579, 655
ErhI CCWWGG 2 cut(s) 842, 850
Esp3I CGTCTC 1 cut(s) 475
FaeI CATG 5 cut(s) 40, 154, 265, 287, 854
FaqI GGGAC 2 cut(s) 579, 749
FatI CATG 5 cut(s) 36, 150, 261, 283, 850
Fnu4HI GCNGC 6 cut(s) 52, 524, 614, 617, 662, 826
FokI GGATG 1 cut(s) 400
Fsp4HI GCNGC 6 cut(s) 52, 524, 614, 617, 662, 826
FspBI CTAG 2 cut(s) 66, 843
GluI GCNGC 6 cut(s) 52, 524, 614, 617, 662, 826
HaeIII GGCC 2 cut(s) 351, 691
HapII CCGG 1 cut(s) 562
HgaI GACGC 1 cut(s) 100
Hin1II CATG 5 cut(s) 40, 154, 265, 287, 854
HinfI GANTC 5 cut(s) 57, 106, 340, 497, 855
HpaII CCGG 1 cut(s) 562
HphI GGTGA 3 cut(s) 40, 457, 492
Hpy188I TCNGA 7 cut(s) 76, 145, 244, 302, 496, 543, 866
Hpy188III TCNNGA 7 cut(s) 128, 151, 209, 218, 284, 335, 518
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 3 cut(s) 148, 468, 472
HpyCH4III ACNGT 5 cut(s) 80, 281, 469, 515, 708
HpyCH4IV ACGT 2 cut(s) 657, 683
HpyCH4V TGCA 9 cut(s) 42, 224, 320, 577, 586, 616, 653, 661, 792
HpyF10VI GCNNNNNNNGC 5 cut(s) 48, 408, 437, 583, 777
HpyF3I CTNAG 2 cut(s) 299, 763
HpySE526I ACGT 2 cut(s) 657, 683
Hsp92II CATG 5 cut(s) 40, 154, 265, 287, 854
KroI GCCGGC 1 cut(s) 561
KroNI GCCGGC 1 cut(s) 563
Kzo9I GATC 3 cut(s) 14, 201, 214
LguI GCTCTTC 2 cut(s) 438, 811
LmnI GCTCC 2 cut(s) 445, 485
Lsp1109I GCAGC 4 cut(s) 600, 628, 673, 837
LweI GCATC 2 cut(s) 211, 735
MaeI CTAG 2 cut(s) 66, 843
MaeII ACGT 2 cut(s) 657, 683
MaeIII GTNAC 1 cut(s) 515
MalI GATC 3 cut(s) 16, 203, 216
MboI GATC 3 cut(s) 14, 201, 214
MboII GAAGA 6 cut(s) 166, 224, 260, 398, 425, 828
MfeI CAATTG 1 cut(s) 554
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 2 cut(s) 192, 442
MluCI AATT 2 cut(s) 554, 714
MlyI GAGTC 3 cut(s) 100, 349, 849
MnlI CCTC 6 cut(s) 71, 262, 493, 675, 716, 843
Mph1103I ATGCAT 3 cut(s) 226, 579, 655
MroNI GCCGGC 1 cut(s) 561
MseI TTAA 1 cut(s) 680
MspA1I CMGCKG 1 cut(s) 613
MspI CCGG 1 cut(s) 562
MunI CAATTG 1 cut(s) 554
Mva1269I GAATGC 1 cut(s) 402
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 5 cut(s) 48, 408, 437, 583, 777
NaeI GCCGGC 1 cut(s) 563
NcoI CCATGG 1 cut(s) 850
NdeII GATC 3 cut(s) 14, 201, 214
NgoMIV GCCGGC 1 cut(s) 561
NlaIII CATG 5 cut(s) 40, 154, 265, 287, 854
NlaIV GGNNCC 3 cut(s) 352, 737, 848
NmeAIII GCCGAG 1 cut(s) 45
NmuCI GTSAC 1 cut(s) 515
NsiI ATGCAT 3 cut(s) 226, 579, 655
PagI TCATGA 2 cut(s) 150, 283
PciSI GCTCTTC 2 cut(s) 438, 811
PctI GAATGC 1 cut(s) 402
PdiI GCCGGC 1 cut(s) 563
PfeI GAWTC 2 cut(s) 57, 497
PkrI GCNGC 6 cut(s) 53, 525, 615, 618, 663, 827
PleI GAGTC 3 cut(s) 100, 348, 849
PpsI GAGTC 3 cut(s) 100, 348, 849
Ppu21I YACGTR 1 cut(s) 658
PshAI GACNNNNGTC 1 cut(s) 110
PspN4I GGNNCC 3 cut(s) 352, 737, 848
PspPI GGNCC 2 cut(s) 350, 736
PstI CTGCAG 1 cut(s) 618
PstNI CAGNNNCTG 1 cut(s) 810
PsuI RGATCY 1 cut(s) 214
PvuII CAGCTG 1 cut(s) 613
RsaI GTAC 1 cut(s) 785
RsaNI GTAC 1 cut(s) 784
SapI GCTCTTC 2 cut(s) 438, 811
SaqAI TTAA 1 cut(s) 680
SatI GCNGC 6 cut(s) 52, 524, 614, 617, 662, 826
Sau3AI GATC 3 cut(s) 14, 201, 214
Sau96I GGNCC 2 cut(s) 350, 736
SchI GAGTC 3 cut(s) 100, 349, 849
SduI GDGCHC 2 cut(s) 192, 442
SetI ASST 9 cut(s) 30, 300, 460, 505, 615, 660, 686, 769, 809
SfaNI GCATC 2 cut(s) 211, 735
SfcI CTRYAG 1 cut(s) 614
SinI GGWCC 1 cut(s) 736
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 2 cut(s) 554, 714
SsiI CCGC 7 cut(s) 5, 51, 99, 524, 771, 802, 859
SspMI CTAG 2 cut(s) 66, 843
StyI CCWWGG 2 cut(s) 842, 850
TaaI ACNGT 5 cut(s) 80, 281, 469, 515, 708
TaiI ACGT 2 cut(s) 660, 686
TaqI TCGA 1 cut(s) 357
TasI AATT 2 cut(s) 554, 714
TauI GCSGC 2 cut(s) 54, 526
TfiI GAWTC 2 cut(s) 57, 497
Tru1I TTAA 1 cut(s) 680
Tru9I TTAA 1 cut(s) 680
TscAI CASTG 2 cut(s) 85, 333
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 4 cut(s) 613, 616, 661, 825
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 4 cut(s) 25, 167, 261, 300
TspRI CASTG 2 cut(s) 85, 333
VpaK11BI GGWCC 1 cut(s) 736
XapI RAATTY 1 cut(s) 714
XmaJI CCTAGG 1 cut(s) 842
XspI CTAG 2 cut(s) 66, 843
Zsp2I ATGCAT 3 cut(s) 226, 579, 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.