FvH4_5g28590

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016581
Physical Location & Seq
Forward (+)
19754285 .. 19756133
1849 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28590.t1

Sequence Viewer

Length: 1104 bp
ATGGCGTGTGAAGTCGGTGACGAGGGATCGGGTTCGGGATCGCTGAAGATCAAGAATAGGCGGCTTAGGATCAAGTTGTTATGCAGCTATGGCGGGAAAATCCTACCCAGACCTTCCGATGGTATTCTTAAGTACGTCGGTGGCGAGACTCGTGTCATTGCCTTGTCCCACGACGCTAATTTTTCTGAGTTGATGAAGAAAGTAAATGCAGTAGTTGAAGGGGACATGGTGTTGAAGTACCAGTTAATCCCGGAAGGTCTGGATGCCTTGGTCTCTGTTCGAAGCGATGAAGATGTAAAGCACATGTTGGACGAATACGATAGACAAGAGAAGCAAGGCATGAGTCCAAGGCTAAGAACCTTCTTGTTTTCATCCAAGCCAATCATGATAGAAAGCAGCAGCAGCAACAACAACAACAACCAGACATTAGAGCTAGAGCAGCGGTATATTGACGCCATTAACGGCATAGTTCGTTCATCATCGAAACAACCAATAATGATGATCGGCGGCAGCCCTCGCTTGAGCAGCAGCTTTGGGAATAATTGCAGCAGCCCCTTAGCCAGCTACAGCCCCCTCGCTAGCTTTTCCTCTGCTTGTTCTTCGCCCAAGTCAGAATCGCCCGAACACCATTATATGGTGGTACAAGAAGCTAACCAGATATTACAAGCTAGTCGATTTTCGCTCCCTCTAGTTATGCATAAAGTGCACAGCTCTCCCAGTATTTGTGGTAATCTTAATCTACAACAACAATACCAAAACCTAAACTTGGCTCACCGTCACCACCACCACCAGTACCCGCAGCAACACCAAAGTGGCCACTATCAAATGACTAGGAGTCTATCAAGAAACTCGCATCCCCAAAACCATAGTAGAGGTACTCCCACCCCACCGCATATGTCCCCCGGGGGAAGATCTGAATCAGGGAGCGTGAGGAGCCTGATGGGTCAAAACCAGTCTCATCATAATTCCACTTGCACTAGTCCAAGACACCAACACCAAAGAATGATAAATATTGCAGCAGTAGGAGGAGGAAGAAGCTATGGTTATTCTGATGATTCTTGTAACATGGTTGATAGGGCTGAGAGTCTTCCAACTAGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

368

Amino Acids

40.55

Weight (kDa)

9.02

Isoelectric Point (pI)

71.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 43 - 113 1.2e-12 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016581)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49920
fragaria_vesca FvH4_5g28590
malus_domestica MD15G1436100.v1.1
prunus_persica Prupe.1G579900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220591
rosa_laevigata RLG00000002215
rosa_multiflora Rmu_ssc0000189.1_g000028
rosa_roxburghii Rroxscaffold_3G00239280
rosa_rugosa Rorug07G0195200
rosa_samantha Rh7AG336200 Rh7BG327300 Rh7CG353900 Rh7DG332500
rosa_wichuraiana Rw7G028680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 634
AciI CCGC 6 cut(s) 61, 93, 442, 507, 797, 890
AclWI GGATC 3 cut(s) 34, 46, 77
AcoI YGGCCR 1 cut(s) 814
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 5 cut(s) 134, 239, 642, 794, 877
AfiI CCNNNNNNNGG 3 cut(s) 119, 634, 766
AflII CTTAAG 1 cut(s) 128
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 2 cut(s) 218, 235
AhdI GACNNNNNGTC 1 cut(s) 834
AhlI ACTAGT 2 cut(s) 977, 1094
AleI CACNNNNGTG 1 cut(s) 810
AluBI AGCT 9 cut(s) 87, 433, 531, 564, 582, 650, 668, 711, 1038
AluI AGCT 9 cut(s) 87, 433, 531, 564, 582, 650, 668, 711, 1038
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 3 cut(s) 140, 277, 960
Alw44I GTGCAC 1 cut(s) 704
AlwI GGATC 3 cut(s) 34, 46, 77
Ama87I CYCGRG 1 cut(s) 902
AoxI GGCC 1 cut(s) 814
ApaLI GTGCAC 1 cut(s) 704
AsuC2I CCSGG 3 cut(s) 251, 903, 904
AsuHPI GGTGA 3 cut(s) 29, 764, 770
AsuII TTCGAA 1 cut(s) 280
AsuNHI GCTAGC 1 cut(s) 578
AvaI CYCGRG 1 cut(s) 902
BaeGI GKGCMC 1 cut(s) 708
BalI TGGCCA 1 cut(s) 816
BauI CACGAG 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 1079
Bbv12I GWGCWC 1 cut(s) 708
BccI CCATC 2 cut(s) 113, 934
BceAI ACGGC 1 cut(s) 478
BcnI CCSGG 3 cut(s) 251, 903, 904
BcoDI GTCTC 3 cut(s) 140, 277, 960
BcuI ACTAGT 2 cut(s) 977, 1094
BfaI CTAG 7 cut(s) 434, 579, 669, 689, 831, 978, 1095
BfmI CTRYAG 1 cut(s) 565
BfrI CTTAAG 1 cut(s) 128
BglII AGATCT 1 cut(s) 911
Bme1390I CCNGG 3 cut(s) 251, 903, 904
BmeRI GACNNNNNGTC 1 cut(s) 834
BmeT110I CYCGRG 1 cut(s) 902
BmiI GGNNCC 1 cut(s) 935
BmrFI CCNGG 3 cut(s) 251, 903, 904
BmrI ACTGGG 1 cut(s) 711
BmsI GCATC 2 cut(s) 253, 862
BmtI GCTAGC 1 cut(s) 582
BmuI ACTGGG 1 cut(s) 711
BoxI GACNNNNGTC 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 1079
Bpu10I CCTNAGC 2 cut(s) 65, 556
Bpu14I TTCGAA 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 541
BpuMI CCSGG 3 cut(s) 251, 903, 904
BsaBI GATNNNNATC 1 cut(s) 916
BsaHI GRCGYC 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 277
BsaJI CCNNGG 5 cut(s) 267, 347, 901, 902, 903
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 634, 766
Bse1I ACTGG 4 cut(s) 241, 717, 790, 952
Bse3DI GCAATG 1 cut(s) 156
Bse8I GATNNNNATC 1 cut(s) 916
BseDI CCNNGG 5 cut(s) 267, 347, 901, 902, 903
BseGI GGATG 3 cut(s) 268, 371, 853
BseJI GATNNNNATC 1 cut(s) 916
BseLI CCNNNNNNNGG 3 cut(s) 119, 634, 766
BseMI GCAATG 1 cut(s) 156
BseMII CTCAG 2 cut(s) 177, 1071
BseNI ACTGG 4 cut(s) 241, 717, 790, 952
BseRI GAGGAG 2 cut(s) 946, 1041
BseSI GKGCMC 1 cut(s) 708
BshFI GGCC 1 cut(s) 816
BsiHKAI GWGCWC 1 cut(s) 708
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 2 cut(s) 251, 903
BslFI GGGAC 3 cut(s) 151, 236, 883
BslI CCNNNNNNNGG 3 cut(s) 119, 634, 766
BsmAI GTCTC 3 cut(s) 140, 277, 960
BsmFI GGGAC 3 cut(s) 151, 236, 883
BsnI GGCC 1 cut(s) 816
Bso31I GGTCTC 1 cut(s) 277
BsoBI CYCGRG 1 cut(s) 902
Bsp119I TTCGAA 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 708
Bsp143I GATC 6 cut(s) 26, 38, 48, 69, 501, 911
BspACI CCGC 6 cut(s) 61, 93, 442, 507, 797, 890
BspANI GGCC 1 cut(s) 816
BspCNI CTCAG 2 cut(s) 178, 1072
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 935
BspOI GCTAGC 1 cut(s) 582
BspPI GGATC 3 cut(s) 34, 46, 77
BspT104I TTCGAA 1 cut(s) 280
BspTI CTTAAG 1 cut(s) 128
BspTNI GGTCTC 1 cut(s) 277
BsrDI GCAATG 1 cut(s) 156
BsrI ACTGG 4 cut(s) 241, 717, 790, 952
BssECI CCNNGG 5 cut(s) 267, 347, 901, 902, 903
BssMI GATC 6 cut(s) 26, 38, 48, 69, 501, 911
BssNI GRCGYC 1 cut(s) 453
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 2 cut(s) 267, 347
Bst2BI CACGAG 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 776
BstACI GRCGYC 1 cut(s) 453
BstAFI CTTAAG 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 703
BstBI TTCGAA 1 cut(s) 280
BstC8I GCNNGC 2 cut(s) 562, 580
BstDEI CTNAG 5 cut(s) 65, 186, 353, 556, 1080
BstF5I GGATG 3 cut(s) 268, 371, 853
BstKTI GATC 6 cut(s) 29, 41, 51, 72, 504, 914
BstMAI GTCTC 3 cut(s) 140, 277, 960
BstMBI GATC 6 cut(s) 26, 38, 48, 69, 501, 911
BstMWI GCNNNNNNNGC 7 cut(s) 90, 402, 439, 516, 525, 703, 933
BstNSI RCATGY 1 cut(s) 307
BstPAI GACNNNNGTC 1 cut(s) 152
BstSCI CCNGG 3 cut(s) 249, 901, 902
BstSFI CTRYAG 1 cut(s) 565
BstSLI GKGCMC 1 cut(s) 708
BstV2I GAAGAC 1 cut(s) 1079
BstX2I RGATCY 1 cut(s) 911
BstYI RGATCY 1 cut(s) 911
BsuRI GGCC 1 cut(s) 816
BtgZI GCGATG 1 cut(s) 300
BtsCI GGATG 3 cut(s) 268, 371, 853
Cac8I GCNNGC 2 cut(s) 562, 580
CciI TCATGA 1 cut(s) 384
Cfr9I CCCGGG 1 cut(s) 902
CseI GACGC 2 cut(s) 182, 461
Csp6I GTAC 5 cut(s) 133, 238, 641, 793, 876
CviAII CATG 6 cut(s) 226, 304, 340, 385, 1066, 1101
CviQI GTAC 5 cut(s) 133, 238, 641, 793, 876
DdeI CTNAG 5 cut(s) 65, 186, 353, 556, 1080
DpnI GATC 6 cut(s) 28, 40, 50, 71, 503, 913
DpnII GATC 6 cut(s) 26, 38, 48, 69, 501, 911
DriI GACNNNNNGTC 1 cut(s) 834
EaeI YGGCCR 1 cut(s) 814
Eam1105I GACNNNNNGTC 1 cut(s) 834
Eco130I CCWWGG 2 cut(s) 267, 347
Eco31I GGTCTC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 902
EcoT14I CCWWGG 2 cut(s) 267, 347
EcoT22I ATGCAT 1 cut(s) 699
ErhI CCWWGG 2 cut(s) 267, 347
FaeI CATG 6 cut(s) 229, 307, 343, 388, 1069, 1104
FaqI GGGAC 3 cut(s) 151, 236, 883
FatI CATG 6 cut(s) 225, 303, 339, 384, 1065, 1100
FauI CCCGC 2 cut(s) 86, 804
FauNDI CATATG 1 cut(s) 894
FokI GGATG 3 cut(s) 275, 358, 840
FspBI CTAG 7 cut(s) 434, 579, 669, 689, 831, 978, 1095
HaeIII GGCC 1 cut(s) 816
HapII CCGG 2 cut(s) 251, 903
HgaI GACGC 2 cut(s) 182, 461
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 6 cut(s) 229, 307, 343, 388, 1069, 1104
HinfI GANTC 7 cut(s) 148, 343, 614, 835, 917, 1055, 1084
HpaII CCGG 2 cut(s) 251, 903
HphI GGTGA 3 cut(s) 29, 764, 770
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 5 cut(s) 118, 187, 613, 916, 1051
Hpy188III TCNNGA 5 cut(s) 36, 52, 260, 385, 843
Hpy8I GTNNAC 1 cut(s) 706
Hpy99I CGWCG 2 cut(s) 140, 176
HpyAV CCTTC 4 cut(s) 123, 212, 248, 370
HpyCH4III ACNGT 1 cut(s) 776
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 7 cut(s) 84, 209, 546, 697, 706, 975, 1016
HpyF10VI GCNNNNNNNGC 7 cut(s) 90, 402, 439, 516, 525, 703, 933
HpyF3I CTNAG 5 cut(s) 65, 186, 353, 556, 1080
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 6 cut(s) 229, 307, 343, 388, 1069, 1104
Kzo9I GATC 6 cut(s) 26, 38, 48, 69, 501, 911
LmnI GCTCC 3 cut(s) 687, 924, 933
LweI GCATC 2 cut(s) 253, 862
MaeI CTAG 7 cut(s) 434, 579, 669, 689, 831, 978, 1095
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 3 cut(s) 17, 776, 1061
MalI GATC 6 cut(s) 28, 40, 50, 71, 503, 913
MboI GATC 6 cut(s) 26, 38, 48, 69, 501, 911
MboII GAAGA 7 cut(s) 58, 208, 302, 591, 921, 1044, 1079
MflI RGATCY 1 cut(s) 911
MhlI GDGCHC 1 cut(s) 708
MlsI TGGCCA 1 cut(s) 816
MluCI AATT 3 cut(s) 178, 541, 964
MluNI TGGCCA 1 cut(s) 816
MlyI GAGTC 4 cut(s) 142, 352, 844, 1093
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 9 cut(s) 16, 525, 584, 598, 696, 866, 924, 1019, 1022
Mox20I TGGCCA 1 cut(s) 816
Mph1103I ATGCAT 1 cut(s) 699
MscI TGGCCA 1 cut(s) 816
MseI TTAA 4 cut(s) 129, 245, 459, 735
MslI CAYNNNNRTG 1 cut(s) 810
Msp20I TGGCCA 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 442
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 2 cut(s) 251, 903
MspR9I CCNGG 3 cut(s) 251, 903, 904
MwoI GCNNNNNNNGC 7 cut(s) 90, 402, 439, 516, 525, 703, 933
NciI CCSGG 3 cut(s) 251, 903, 904
NdeI CATATG 1 cut(s) 894
NdeII GATC 6 cut(s) 26, 38, 48, 69, 501, 911
NheI GCTAGC 1 cut(s) 578
NlaIII CATG 6 cut(s) 229, 307, 343, 388, 1069, 1104
NlaIV GGNNCC 1 cut(s) 935
NmuCI GTSAC 2 cut(s) 17, 776
NsiI ATGCAT 1 cut(s) 699
NspI RCATGY 1 cut(s) 307
NspV TTCGAA 1 cut(s) 280
OliI CACNNNNGTG 1 cut(s) 810
PagI TCATGA 1 cut(s) 384
PciI ACATGT 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 3 cut(s) 614, 917, 1055
PflMI CCANNNNNTGG 1 cut(s) 634
PfoI TCCNGGA 1 cut(s) 249
PleI GAGTC 4 cut(s) 142, 351, 843, 1092
PpsI GAGTC 4 cut(s) 142, 351, 843, 1092
PscI ACATGT 1 cut(s) 303
PshAI GACNNNNGTC 1 cut(s) 152
PspN4I GGNNCC 1 cut(s) 935
PsuI RGATCY 1 cut(s) 911
RsaI GTAC 5 cut(s) 134, 239, 642, 794, 877
RsaNI GTAC 5 cut(s) 133, 238, 641, 793, 876
RseI CAYNNNNRTG 1 cut(s) 810
SaqAI TTAA 4 cut(s) 129, 245, 459, 735
Sau3AI GATC 6 cut(s) 26, 38, 48, 69, 501, 911
SchI GAGTC 4 cut(s) 142, 352, 844, 1093
ScrFI CCNGG 3 cut(s) 251, 903, 904
SduI GDGCHC 1 cut(s) 708
SfaNI GCATC 2 cut(s) 253, 862
SfcI CTRYAG 1 cut(s) 565
SfuI TTCGAA 1 cut(s) 280
SmaI CCCGGG 1 cut(s) 904
SmiMI CAYNNNNRTG 1 cut(s) 810
SmlI CTYRAG 2 cut(s) 128, 520
SmoI CTYRAG 2 cut(s) 128, 520
SpeI ACTAGT 2 cut(s) 977, 1094
Sse9I AATT 3 cut(s) 178, 541, 964
SsiI CCGC 6 cut(s) 61, 93, 442, 507, 797, 890
SspI AATATT 1 cut(s) 1012
SspMI CTAG 7 cut(s) 434, 579, 669, 689, 831, 978, 1095
StyD4I CCNGG 3 cut(s) 249, 901, 902
StyI CCWWGG 2 cut(s) 267, 347
TaaI ACNGT 1 cut(s) 776
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 280, 482, 673
TasI AATT 3 cut(s) 178, 541, 964
TauI GCSGC 2 cut(s) 64, 510
TfiI GAWTC 3 cut(s) 614, 917, 1055
Tru1I TTAA 4 cut(s) 129, 245, 459, 735
Tru9I TTAA 4 cut(s) 129, 245, 459, 735
TseFI GTSAC 2 cut(s) 17, 776
Tsp45I GTSAC 2 cut(s) 17, 776
TspDTI ATGAA 4 cut(s) 209, 303, 360, 465
TspMI CCCGGG 1 cut(s) 902
Van91I CCANNNNNTGG 1 cut(s) 634
Vha464I CTTAAG 1 cut(s) 128
VneI GTGCAC 1 cut(s) 704
XceI RCATGY 1 cut(s) 307
XmaI CCCGGG 1 cut(s) 902
XspI CTAG 7 cut(s) 434, 579, 669, 689, 831, 978, 1095
Zsp2I ATGCAT 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.