MD15G1436100.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016581
Physical Location & Seq
Forward (+)
53634141 .. 53635381
1241 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1436100.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGCGGGTGAAATTGGTGGCACTGGTGGCGGTAGTTGCGGTGGTGAGAATGACGCGCCGTCCTCCCCCAAAAACCGGATCAAGTTCTTGTGCAGCCATGGCGGCAGAATCCTACCCAGGCCTGCCGACGGCCACCTCAAGTATGTTGGAGGGGAGACTCGCGTCATTGCATTGTCCCGCGGCATAACATACGCAGAGTTGATGGAAAAGCTGAGTACATTGTCTGATGGGGAGATGGTGTTGAAATACCAGCCGGCCAATGAAGATCTCGATGCCTTGGTCTCCGTGAGAAGCGACGAAGATCTAAAGCACATGCTCGACGAATATGATCGACAAGAGAGCGAGGGCAGCCCGAGGCCGAGAGCATTCTTGTTCCCGTCCAAGTTAATCATGATCGAGAGTCCAACCCCGTCGTTGGAACACCAGCTGGAGCAGCGTTACATTGACGCCATCAATGGAATCATCCGATCGCCACCAAAAATGCAGCCTCAACCGCTGCAGCAACAGCAGCAGTTTGCTCCGGTTGCCCCAAAGGTCATCGCAAGTAGCCCCATGGGCAGCTTCGGCTTATATTCTGCATGTTCATCGCCCAAATCAGCATCTCAATCAGCATCCCCTGAACACCACGCGGTTGTGGCTGACATGAGCATGGCAGGTCACCGGCTCACACTTTCGCCTCCAGTGCATAGAGTGCACAGCTCTCCAAGTATTTGCAGCGCATACCAAGGCCATGTTCAAGGGCGTCTCCAACACCCTAACTACCACCAAAACCACCACCACCACCACCAGGCGTACCAGTCTTCGTTTAGTGCAAGAGCGCACCACCGAGGGATTAGGACTCCACCTCCTTTGACGTCGCCAGGTAGATCAGAGTCTGGAAGCGCGAGGTGCTCGTTGATGGGGCCGAATATTTCTCCGGGTCAAGGCCAAAATCATATTCCTTCCAACTTATCTCCTAGTAGCAACAACAGCAGTCCTAGATCATCACTGAGACTTAGCGGTGGCGGAGGAGGACGAGGTCATGAAAGCCTCCCTCGAAGTCCTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

350

Amino Acids

37.57

Weight (kDa)

9.11

Isoelectric Point (pI)

73.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 44 - 126 4.2e-13 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016581)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49920
fragaria_vesca FvH4_5g28590
malus_domestica MD15G1436100.v1.1
prunus_persica Prupe.1G579900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220591
rosa_laevigata RLG00000002215
rosa_multiflora Rmu_ssc0000189.1_g000028
rosa_roxburghii Rroxscaffold_3G00239280
rosa_rugosa Rorug07G0195200
rosa_samantha Rh7AG336200 Rh7BG327300 Rh7CG353900 Rh7DG332500
rosa_wichuraiana Rw7G028680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 857
Acc36I ACCTGC 1 cut(s) 644
AccII CGCG 5 cut(s) 56, 162, 180, 629, 884
AclWI GGATC 1 cut(s) 86
AcoI YGGCCR 2 cut(s) 130, 255
AcyI GRCGYC 3 cut(s) 447, 742, 854
AfaI GTAC 2 cut(s) 217, 794
AfiI CCNNNNNNNGG 5 cut(s) 75, 128, 415, 787, 923
AgsI TTSAA 2 cut(s) 244, 737
AhdI GACNNNNNGTC 1 cut(s) 58
AjnI CCWGG 3 cut(s) 116, 786, 859
AjuI GAANNNNNNNTTGG 4 cut(s) 717, 749, 921, 953
AluBI AGCT 4 cut(s) 211, 427, 561, 699
AluI AGCT 4 cut(s) 211, 427, 561, 699
Alw21I GWGCWC 2 cut(s) 696, 893
Alw26I GTCTC 4 cut(s) 149, 286, 749, 985
Alw44I GTGCAC 1 cut(s) 692
AlwI GGATC 1 cut(s) 86
AlwNI CAGNNNCTG 1 cut(s) 875
Ama87I CYCGRG 1 cut(s) 352
AoxI GGCC 7 cut(s) 119, 130, 255, 356, 727, 902, 925
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 9 cut(s) 93, 348, 433, 484, 496, 499, 508, 558, 714
AspLEI GCGC 4 cut(s) 58, 719, 820, 884
AspS9I GGNCC 1 cut(s) 902
AsuC2I CCSGG 1 cut(s) 918
AsuHPI GGTGA 3 cut(s) 20, 56, 650
AvaI CYCGRG 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 792
Bbv12I GWGCWC 2 cut(s) 696, 893
BbvI GCAGC 9 cut(s) 105, 360, 445, 483, 496, 511, 520, 570, 726
BccI CCATC 5 cut(s) 196, 221, 229, 458, 892
BceAI ACGGC 2 cut(s) 43, 145
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciT130I CCWGG 3 cut(s) 118, 788, 861
BcnI CCSGG 1 cut(s) 918
BcoDI GTCTC 4 cut(s) 149, 286, 749, 985
BfaI CTAG 3 cut(s) 957, 978, 1044
BfmI CTRYAG 1 cut(s) 497
BfuAI ACCTGC 1 cut(s) 644
BglI GCCNNNNNGGC 2 cut(s) 102, 555
BglII AGATCT 2 cut(s) 265, 301
Bme1390I CCNGG 4 cut(s) 118, 788, 861, 918
BmeRI GACNNNNNGTC 1 cut(s) 58
BmeT110I CYCGRG 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 902
BmiI GGNNCC 1 cut(s) 903
BmrFI CCNGG 4 cut(s) 118, 788, 861, 918
BmsI GCATC 3 cut(s) 262, 608, 620
BoxI GACNNNNGTC 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 792
BpmI CTGGAG 2 cut(s) 449, 663
BpuEI CTTGAG 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 918
BsaHI GRCGYC 3 cut(s) 447, 742, 854
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 8 cut(s) 97, 116, 178, 276, 353, 552, 724, 826
BsaWI WCCGGW 2 cut(s) 75, 520
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 829, 859
Bsc4I CCNNNNNNNGG 5 cut(s) 75, 128, 415, 787, 923
Bse118I RCCGGY 2 cut(s) 253, 660
Bse1I ACTGG 3 cut(s) 28, 680, 796
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 3 cut(s) 118, 788, 861
BseDI CCNNGG 8 cut(s) 97, 116, 178, 276, 353, 552, 724, 826
BseGI GGATG 2 cut(s) 462, 611
BseLI CCNNNNNNNGG 5 cut(s) 75, 128, 415, 787, 923
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 2 cut(s) 203, 980
BseNI ACTGG 3 cut(s) 28, 680, 796
BseRI GAGGAG 1 cut(s) 1023
BseSI GKGCMC 1 cut(s) 696
BseXI GCAGC 9 cut(s) 105, 360, 445, 483, 496, 511, 520, 570, 726
BsgI GTGCAG 1 cut(s) 112
Bsh1236I CGCG 5 cut(s) 56, 162, 180, 629, 884
Bsh1285I CGRYCG 1 cut(s) 470
BshFI GGCC 7 cut(s) 121, 132, 257, 358, 729, 904, 927
BsiEI CGRYCG 1 cut(s) 470
BsiHKAI GWGCWC 2 cut(s) 696, 893
BsiHKCI CYCGRG 1 cut(s) 352
BsiSI CCGG 5 cut(s) 76, 254, 521, 661, 917
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 5 cut(s) 75, 128, 415, 787, 923
BsmAI GTCTC 4 cut(s) 149, 286, 749, 985
BsmBI CGTCTC 1 cut(s) 749
BsmFI GGGAC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 7 cut(s) 121, 132, 257, 358, 729, 904, 927
Bso31I GGTCTC 1 cut(s) 286
BsoBI CYCGRG 1 cut(s) 352
Bsp1286I GDGCHC 2 cut(s) 696, 893
Bsp143I GATC 8 cut(s) 78, 265, 301, 328, 393, 467, 866, 980
Bsp19I CCATGG 2 cut(s) 97, 552
BspANI GGCC 7 cut(s) 121, 132, 257, 358, 729, 904, 927
BspCNI CTCAG 2 cut(s) 204, 981
BspFNI CGCG 5 cut(s) 56, 162, 180, 629, 884
BspHI TCATGA 2 cut(s) 390, 1021
BspLI GGNNCC 1 cut(s) 903
BspMAI CTGCAG 1 cut(s) 501
BspMI ACCTGC 1 cut(s) 644
BspPI GGATC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 2 cut(s) 253, 660
BsrI ACTGG 3 cut(s) 28, 680, 796
BssAI RCCGGY 2 cut(s) 253, 660
BssECI CCNNGG 8 cut(s) 97, 116, 178, 276, 353, 552, 724, 826
BssMI GATC 8 cut(s) 78, 265, 301, 328, 393, 467, 866, 980
BssNI GRCGYC 3 cut(s) 447, 742, 854
BssT1I CCWWGG 4 cut(s) 97, 276, 552, 724
Bst2UI CCWGG 3 cut(s) 118, 788, 861
BstACI GRCGYC 3 cut(s) 447, 742, 854
BstAPI GCANNNNNTGC 1 cut(s) 691
BstC8I GCNNGC 2 cut(s) 123, 255
BstDEI CTNAG 3 cut(s) 212, 989, 995
BstDSI CCRYGG 3 cut(s) 97, 178, 552
BstEII GGTNACC 1 cut(s) 656
BstF5I GGATG 2 cut(s) 462, 611
BstFNI CGCG 5 cut(s) 56, 162, 180, 629, 884
BstHHI GCGC 4 cut(s) 58, 719, 820, 884
BstKTI GATC 8 cut(s) 81, 268, 304, 331, 396, 470, 869, 983
BstMAI GTCTC 4 cut(s) 149, 286, 749, 985
BstMBI GATC 8 cut(s) 78, 265, 301, 328, 393, 467, 866, 980
BstMCI CGRYCG 1 cut(s) 470
BstNI CCWGG 3 cut(s) 118, 788, 861
BstNSI RCATGY 2 cut(s) 316, 582
BstPAI GACNNNNGTC 1 cut(s) 161
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 4 cut(s) 116, 786, 859, 916
BstSFI CTRYAG 1 cut(s) 497
BstSLI GKGCMC 1 cut(s) 696
BstUI CGCG 5 cut(s) 56, 162, 180, 629, 884
BstV1I GCAGC 9 cut(s) 105, 360, 445, 483, 496, 511, 520, 570, 726
BstV2I GAAGAC 1 cut(s) 792
BstX2I RGATCY 2 cut(s) 265, 301
BstYI RGATCY 2 cut(s) 265, 301
BsuRI GGCC 7 cut(s) 121, 132, 257, 358, 729, 904, 927
BtgI CCRYGG 3 cut(s) 97, 178, 552
BtgZI GCGATG 2 cut(s) 523, 570
BtsCI GGATG 2 cut(s) 462, 611
BtsIMutI CAGTG 3 cut(s) 21, 687, 986
BveI ACCTGC 1 cut(s) 644
Cac8I GCNNGC 2 cut(s) 123, 255
CaiI CAGNNNCTG 1 cut(s) 875
CciI TCATGA 2 cut(s) 390, 1021
CfoI GCGC 4 cut(s) 58, 719, 820, 884
Cfr10I RCCGGY 2 cut(s) 253, 660
Cfr13I GGNCC 1 cut(s) 902
Cfr42I CCGCGG 1 cut(s) 181
CseI GACGC 4 cut(s) 62, 151, 455, 731
Csp6I GTAC 2 cut(s) 216, 793
CspCI CAANNNNNGTGG 2 cut(s) 831, 866
CviAII CATG 9 cut(s) 98, 313, 391, 553, 579, 643, 649, 731, 1022
CviQI GTAC 2 cut(s) 216, 793
DdeI CTNAG 3 cut(s) 212, 989, 995
DpnI GATC 8 cut(s) 80, 267, 303, 330, 395, 469, 868, 982
DpnII GATC 8 cut(s) 78, 265, 301, 328, 393, 467, 866, 980
DriI GACNNNNNGTC 1 cut(s) 58
EaeI YGGCCR 2 cut(s) 130, 255
Eam1105I GACNNNNNGTC 1 cut(s) 58
EciI GGCGGA 1 cut(s) 1020
Eco130I CCWWGG 4 cut(s) 97, 276, 552, 724
Eco147I AGGCCT 1 cut(s) 121
Eco31I GGTCTC 1 cut(s) 286
Eco88I CYCGRG 1 cut(s) 352
Eco91I GGTNACC 1 cut(s) 656
EcoO65I GGTNACC 1 cut(s) 656
EcoRII CCWGG 3 cut(s) 116, 786, 859
EcoT14I CCWWGG 4 cut(s) 97, 276, 552, 724
ErhI CCWWGG 4 cut(s) 97, 276, 552, 724
Esp3I CGTCTC 1 cut(s) 749
FaeI CATG 9 cut(s) 101, 316, 394, 556, 582, 646, 652, 734, 1025
FaqI GGGAC 1 cut(s) 160
FatI CATG 9 cut(s) 97, 312, 390, 552, 578, 642, 648, 730, 1021
FauI CCCGC 1 cut(s) 185
FokI GGATG 2 cut(s) 449, 598
FspBI CTAG 3 cut(s) 957, 978, 1044
GlaI GCGC 4 cut(s) 57, 718, 819, 883
GsuI CTGGAG 2 cut(s) 449, 663
HaeIII GGCC 7 cut(s) 121, 132, 257, 358, 729, 904, 927
HapII CCGG 5 cut(s) 76, 254, 521, 661, 917
HgaI GACGC 4 cut(s) 62, 151, 455, 731
HhaI GCGC 4 cut(s) 58, 719, 820, 884
Hin1I GRCGYC 3 cut(s) 447, 742, 854
Hin1II CATG 9 cut(s) 101, 316, 394, 556, 582, 646, 652, 734, 1025
Hin6I GCGC 4 cut(s) 56, 717, 818, 882
HinP1I GCGC 4 cut(s) 56, 717, 818, 882
HinfI GANTC 6 cut(s) 108, 157, 400, 459, 838, 872
HpaII CCGG 5 cut(s) 76, 254, 521, 661, 917
HphI GGTGA 3 cut(s) 20, 56, 650
Hpy166II GTNNAC 1 cut(s) 694
Hpy188I TCNGA 3 cut(s) 226, 467, 871
Hpy188III TCNNGA 5 cut(s) 269, 391, 397, 876, 1022
Hpy8I GTNNAC 1 cut(s) 694
Hpy99I CGWCG 5 cut(s) 131, 299, 323, 415, 859
HpyAV CCTTC 1 cut(s) 951
HpyCH4IV ACGT 1 cut(s) 854
HpyCH4V TGCA 9 cut(s) 93, 170, 484, 499, 578, 685, 694, 714, 812
HpyF3I CTNAG 3 cut(s) 212, 989, 995
HpySE526I ACGT 1 cut(s) 854
Hsp92I GRCGYC 3 cut(s) 447, 742, 854
Hsp92II CATG 9 cut(s) 101, 316, 394, 556, 582, 646, 652, 734, 1025
HspAI GCGC 4 cut(s) 56, 717, 818, 882
KroI GCCGGC 1 cut(s) 253
KroNI GCCGGC 1 cut(s) 255
KspI CCGCGG 1 cut(s) 181
Kzo9I GATC 8 cut(s) 78, 265, 301, 328, 393, 467, 866, 980
LmnI GCTCC 2 cut(s) 430, 523
Lsp1109I GCAGC 9 cut(s) 105, 360, 445, 483, 496, 511, 520, 570, 726
LweI GCATC 3 cut(s) 262, 608, 620
MaeI CTAG 3 cut(s) 957, 978, 1044
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 2 cut(s) 437, 656
MalI GATC 8 cut(s) 80, 267, 303, 330, 395, 469, 868, 982
MboI GATC 8 cut(s) 78, 265, 301, 328, 393, 467, 866, 980
MboII GAAGA 3 cut(s) 275, 311, 792
MflI RGATCY 2 cut(s) 265, 301
MhlI GDGCHC 2 cut(s) 696, 893
MluCI AATT 1 cut(s) 12
MlyI GAGTC 4 cut(s) 151, 409, 832, 881
MmeI TCCRAC 5 cut(s) 127, 396, 428, 772, 969
MroNI GCCGGC 1 cut(s) 253
MseI TTAA 1 cut(s) 386
MslI CAYNNNNRTG 1 cut(s) 647
MspA1I CMGCKG 3 cut(s) 180, 427, 496
MspI CCGG 5 cut(s) 76, 254, 521, 661, 917
MspR9I CCNGG 4 cut(s) 118, 788, 861, 918
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 3 cut(s) 118, 788, 861
MvnI CGCG 5 cut(s) 56, 162, 180, 629, 884
NaeI GCCGGC 1 cut(s) 255
NciI CCSGG 1 cut(s) 918
NcoI CCATGG 2 cut(s) 97, 552
NdeII GATC 8 cut(s) 78, 265, 301, 328, 393, 467, 866, 980
NgoMIV GCCGGC 1 cut(s) 253
NlaIII CATG 9 cut(s) 101, 316, 394, 556, 582, 646, 652, 734, 1025
NlaIV GGNNCC 1 cut(s) 903
NmeAIII GCCGAG 1 cut(s) 384
NmuCI GTSAC 1 cut(s) 656
NspI RCATGY 2 cut(s) 316, 582
PagI TCATGA 2 cut(s) 390, 1021
PceI AGGCCT 1 cut(s) 121
PctI GAATGC 1 cut(s) 365
PdiI GCCGGC 1 cut(s) 255
PfeI GAWTC 2 cut(s) 108, 459
PflFI GACNNNGTC 1 cut(s) 1017
Ple19I CGATCG 1 cut(s) 470
PleI GAGTC 4 cut(s) 151, 408, 832, 880
PpsI GAGTC 4 cut(s) 151, 408, 832, 880
PshAI GACNNNNGTC 1 cut(s) 161
Psp6I CCWGG 3 cut(s) 116, 786, 859
PspEI GGTNACC 1 cut(s) 656
PspGI CCWGG 3 cut(s) 116, 786, 859
PspN4I GGNNCC 1 cut(s) 903
PspPI GGNCC 1 cut(s) 902
PstI CTGCAG 1 cut(s) 501
PstNI CAGNNNCTG 1 cut(s) 875
PsuI RGATCY 2 cut(s) 265, 301
PsyI GACNNNGTC 1 cut(s) 1017
PvuI CGATCG 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 427
RsaI GTAC 2 cut(s) 217, 794
RsaNI GTAC 2 cut(s) 216, 793
RseI CAYNNNNRTG 1 cut(s) 647
SacII CCGCGG 1 cut(s) 181
SaqAI TTAA 1 cut(s) 386
Sau3AI GATC 8 cut(s) 78, 265, 301, 328, 393, 467, 866, 980
Sau96I GGNCC 1 cut(s) 902
SchI GAGTC 4 cut(s) 151, 409, 832, 881
ScrFI CCNGG 4 cut(s) 118, 788, 861, 918
SduI GDGCHC 2 cut(s) 696, 893
SfaNI GCATC 3 cut(s) 262, 608, 620
SfcI CTRYAG 1 cut(s) 497
Sfr303I CCGCGG 1 cut(s) 181
SgrBI CCGCGG 1 cut(s) 181
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 1 cut(s) 12
SseBI AGGCCT 1 cut(s) 121
SspI AATATT 1 cut(s) 910
SspMI CTAG 3 cut(s) 957, 978, 1044
StuI AGGCCT 1 cut(s) 121
StyD4I CCNGG 4 cut(s) 116, 786, 859, 916
StyI CCWWGG 4 cut(s) 97, 276, 552, 724
TaiI ACGT 1 cut(s) 857
TaqI TCGA 5 cut(s) 270, 318, 331, 396, 1036
TasI AATT 1 cut(s) 12
TatI WGTACW 1 cut(s) 215
TauI GCSGC 2 cut(s) 105, 183
TfiI GAWTC 2 cut(s) 108, 459
Tru1I TTAA 1 cut(s) 386
Tru9I TTAA 1 cut(s) 386
TscAI CASTG 3 cut(s) 28, 687, 993
TseFI GTSAC 1 cut(s) 656
TseI GCWGC 9 cut(s) 93, 348, 433, 484, 496, 499, 508, 558, 714
Tsp45I GTSAC 1 cut(s) 656
TspDTI ATGAA 3 cut(s) 276, 573, 1038
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 3 cut(s) 28, 687, 993
Tth111I GACNNNGTC 1 cut(s) 1017
VneI GTGCAC 1 cut(s) 692
XceI RCATGY 2 cut(s) 316, 582
XspI CTAG 3 cut(s) 957, 978, 1044
ZraI GACGTC 1 cut(s) 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.