Rorug07G0195200

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0016581
Physical Location & Seq
Reverse (-)
16663263 .. 16666109
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0195200.1

Sequence Viewer

Length: 1419 bp
ATGACAACCCACTGTCTCCTCTCCATAGTGCCAATCCGTGTTCTCTCCAAATCTGCCGCCCACAGTTCCATAATGCAACCGCCGACCACCCGCAACGTGGCGGTGATCGGCGCCGGAGCAGCCGGCCTGGTCGCCGCGCGCGATCTCCGGCGAGAAGGCCACAAAGTTGTGGTCTTTGAGCGAGGAGACCAGGTGGGTGGCACCTGGGTCTACACTCCCAAGGTCGACTCCGACCCAATTGGGCTCGACCCAGATAGGACCGTGGTCCACTCGAGCCTGTACCAGTCGCTCAGGACCAACCTGCCGAGAGAGGTCATGGGGTTCCGGGACTACCCATTTGTGGAAAAGGAAGGAGATGAAGAGAGAGACCCGAGAAGGTTTCCGGGTCACAGAGAGGTGTTGATGTATTTGAAGGACTTTGCTAAGGACTTTGGGATTTATGGGATTGTGAGGTTTGAGACGGAGGTGGTGTTTGTGGGTTTGGTGGAGGGTGGGAAATGGGAGGTGAAGACTAAGAGTAAGAGAGGCGAGGATTTTGATGAGGTTTATGATGCTGTGGTTGTATGTAATGGTCATTATACTGAGCCTCGCATTGCCCAAATTCCAGGCATTGATACATGGAAAGGGAAGCAAATTCACTGCCATAATTATCGTACACCTGAGCCATATCGAGACCAGGTTGTAATCTTAATAGGGAGCTCTGCTAGTGCTGTTGATATTTCTCGGGACATAGCTGGAGTTGCCAAAGAAGTCCACATTGCATCTAGATCTCTTCCTGATGAAACCTTTGAAAAGATGCCTGGCTATGATAACATGTGGCTTCATTCTATGATCAAAAGTCTCAATGAAGATGGTAGTGCCATTTTCCCAGATGGGAGTGTTGTCCATGCTGACATTATTCTTCAATGTACAGGGTACATTTACGATTTCCCTTTTTTGGAAACCAATGGCACCGTGACTGTGGATGACAACCGTGTGGGGCCACTATACAAGCATGTCTTCCCACCAGCCTTGGCTCCGTCACTATCCTTTGTTGGGTTACCATGGAAGGTTGTTCCTTTCCCCTTGTTCGAATTACAAAGCAAGTGGATAGCAGGTCTTTTATCTACTCGAATTACACTTCCATCTGAAGAGGAGATGATGGAGGATGTCAGAGCATTCTACACTTTACTTGAAGCTTCTGGCGTACCTAAACGATACACTCATAACATGGGTGATTATCAGTTTGAATATGATGACTGGCTTGCTGCTCAATGTGGTTCTCCAGTATCTGAAGAATGGAGAAAGCAAATGTACTTGGCAGTTTCAAAGAATAGGCGTGCCCGACCAGAGAGATACCGGGATGAGTGGGAAGATGACCATTTAGTATTGCAAGCTCGTGAAGACTTCAAGAAATACACCTCAGTCATGGATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

472

Amino Acids

53.61

Weight (kDa)

5.6

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 31 - 257 3.7e-37 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 32 - 321 1.8e-14 Pyridine nucleotide-disulphide oxidoreductase
HI0933_like PF03486 32 - 66 6.7e-06 HI0933-like protein Rossmann domain
DAO PF01266 33 - 68 5.9e-08 FAD dependent oxidoreductase
FAD_binding_2 PF00890 33 - 70 2.4e-07 FAD binding domain
FAD_oxidored PF12831 33 - 71 2.8e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 35 - 69 2.1e-10 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 41 - 134 5.5e-08 Flavin containing amine oxidoreductase
Pyr_redox_3 PF13738 99 - 269 3.9e-13 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 274 - 407 1.1e-16 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016581)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49920
fragaria_vesca FvH4_5g28590
malus_domestica MD15G1436100.v1.1
prunus_persica Prupe.1G579900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220591
rosa_laevigata RLG00000002215
rosa_multiflora Rmu_ssc0000189.1_g000028
rosa_roxburghii Rroxscaffold_3G00239280
rosa_rugosa Rorug07G0195200
rosa_samantha Rh7AG336200 Rh7BG327300 Rh7CG353900 Rh7DG332500
rosa_wichuraiana Rw7G028680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 309, 1085
AccB1I GGYRCC 3 cut(s) 110, 200, 950
AccI GTMKAC 2 cut(s) 210, 225
AccII CGCG 3 cut(s) 137, 139, 141
AciI CCGC 5 cut(s) 57, 80, 91, 101, 135
AcsI RAATTY 2 cut(s) 600, 633
AcuI CTGAAG 2 cut(s) 1149, 1293
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 6 cut(s) 281, 655, 910, 917, 1188, 1295
AfiI CCNNNNNNNGG 4 cut(s) 97, 340, 937, 1035
AflIII ACRYGT 1 cut(s) 813
AgsI TTSAA 7 cut(s) 412, 791, 905, 1175, 1229, 1308, 1390
AjnI CCWGG 6 cut(s) 126, 189, 203, 604, 675, 799
AluBI AGCT 4 cut(s) 699, 734, 1178, 1376
AluI AGCT 4 cut(s) 699, 734, 1178, 1376
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 6 cut(s) 20, 180, 360, 452, 666, 845
AlwNI CAGNNNCTG 1 cut(s) 1271
Ama87I CYCGRG 3 cut(s) 271, 370, 723
AoxI GGCC 3 cut(s) 124, 157, 980
ApeKI GCWGC 2 cut(s) 119, 1247
ApoI RAATTY 2 cut(s) 600, 633
ArsI GACNNNNNNTTYG 4 cut(s) 156, 188, 320, 352
AspLEI GCGC 3 cut(s) 113, 139, 141
AspS9I GGNCC 4 cut(s) 258, 265, 294, 980
AsuC2I CCSGG 3 cut(s) 326, 384, 1340
AsuHPI GGTGA 3 cut(s) 115, 517, 1226
AsuII TTCGAA 1 cut(s) 1071
AvaI CYCGRG 3 cut(s) 271, 370, 723
AvaII GGWCC 3 cut(s) 258, 265, 294
BaeGI GKGCMC 1 cut(s) 1324
BanI GGYRCC 3 cut(s) 110, 200, 950
BanII GRGCYC 2 cut(s) 246, 701
BauI CACGAG 1 cut(s) 1377
BbsI GAAGAC 3 cut(s) 515, 991, 1389
Bbv12I GWGCWC 1 cut(s) 701
BbvI GCAGC 2 cut(s) 131, 1234
BccI CCATC 4 cut(s) 845, 866, 1132, 1135
BciT130I CCWGG 6 cut(s) 128, 191, 205, 606, 677, 801
BclI TGATCA 1 cut(s) 831
BcnI CCSGG 3 cut(s) 326, 384, 1340
BcoDI GTCTC 6 cut(s) 20, 180, 360, 452, 666, 845
BfaI CTAG 2 cut(s) 705, 765
BfoI RGCGCY 1 cut(s) 114
BfuAI ACCTGC 2 cut(s) 309, 1085
BglII AGATCT 1 cut(s) 767
BisI GCNGC 4 cut(s) 57, 120, 135, 1248
BlsI GCNGC 4 cut(s) 58, 121, 136, 1249
Bme1390I CCNGG 9 cut(s) 128, 191, 205, 326, 384, 606, 677, 801, 1340
Bme18I GGWCC 3 cut(s) 258, 265, 294
BmeT110I CYCGRG 3 cut(s) 271, 370, 723
BmgT120I GGNCC 4 cut(s) 258, 265, 294, 980
BmiI GGNNCC 6 cut(s) 112, 202, 323, 952, 981, 1017
BmrFI CCNGG 9 cut(s) 128, 191, 205, 326, 384, 606, 677, 801, 1340
BmsI GCATC 3 cut(s) 541, 770, 786
BoxI GACNNNNGTC 1 cut(s) 263
BpiI GAAGAC 3 cut(s) 515, 991, 1389
BpmI CTGGAG 2 cut(s) 756, 1248
Bpu10I CCTNAGC 3 cut(s) 290, 423, 660
Bpu14I TTCGAA 1 cut(s) 1071
BpuMI CCSGG 3 cut(s) 326, 384, 1340
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 3 cut(s) 180, 360, 666
BsaJI CCNNGG 5 cut(s) 204, 219, 261, 1011, 1043
BsaXI ACNNNNNCTCC 4 cut(s) 177, 207, 455, 485
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 340, 937, 1035
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 3 cut(s) 283, 1244, 1265
Bse3DI GCAATG 2 cut(s) 591, 756
BseBI CCWGG 6 cut(s) 128, 191, 205, 606, 677, 801
BseDI CCNNGG 5 cut(s) 204, 219, 261, 1011, 1043
BseGI GGATG 3 cut(s) 970, 1153, 1348
BseLI CCNNNNNNNGG 4 cut(s) 97, 340, 937, 1035
BseMI GCAATG 2 cut(s) 591, 756
BseMII CTCAG 4 cut(s) 304, 573, 651, 1416
BseNI ACTGG 3 cut(s) 283, 1244, 1265
BsePI GCGCGC 1 cut(s) 137
BseRI GAGGAG 3 cut(s) 8, 198, 1148
BseSI GKGCMC 1 cut(s) 1324
BseXI GCAGC 2 cut(s) 131, 1234
Bsh1236I CGCG 3 cut(s) 137, 139, 141
BshFI GGCC 3 cut(s) 126, 159, 982
BshNI GGYRCC 3 cut(s) 110, 200, 950
BsiHKAI GWGCWC 1 cut(s) 701
BsiHKCI CYCGRG 3 cut(s) 271, 370, 723
BsiSI CCGG 6 cut(s) 114, 123, 148, 325, 383, 1339
BslFI GGGAC 2 cut(s) 341, 740
BslI CCNNNNNNNGG 4 cut(s) 97, 340, 937, 1035
BsmAI GTCTC 6 cut(s) 20, 180, 360, 452, 666, 845
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 2 cut(s) 341, 740
BsmI GAATGC 1 cut(s) 1157
BsnI GGCC 3 cut(s) 126, 159, 982
Bso31I GGTCTC 3 cut(s) 180, 360, 666
BsoBI CYCGRG 3 cut(s) 271, 370, 723
Bsp119I TTCGAA 1 cut(s) 1071
Bsp1286I GDGCHC 3 cut(s) 246, 701, 1324
Bsp1407I TGTACA 1 cut(s) 908
Bsp143I GATC 4 cut(s) 105, 142, 767, 831
Bsp19I CCATGG 1 cut(s) 1043
BspACI CCGC 5 cut(s) 57, 80, 91, 101, 135
BspANI GGCC 3 cut(s) 126, 159, 982
BspCNI CTCAG 4 cut(s) 303, 574, 652, 1415
BspFNI CGCG 3 cut(s) 137, 139, 141
BspLI GGNNCC 6 cut(s) 112, 202, 323, 952, 981, 1017
BspMI ACCTGC 2 cut(s) 309, 1085
BspT104I TTCGAA 1 cut(s) 1071
BspT107I GGYRCC 3 cut(s) 110, 200, 950
BspTNI GGTCTC 3 cut(s) 180, 360, 666
BsrDI GCAATG 2 cut(s) 591, 756
BsrFI RCCGGY 1 cut(s) 122
BsrGI TGTACA 1 cut(s) 908
BsrI ACTGG 3 cut(s) 283, 1244, 1265
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 5 cut(s) 204, 219, 261, 1011, 1043
BssHII GCGCGC 1 cut(s) 137
BssMI GATC 4 cut(s) 105, 142, 767, 831
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 1377
BssT1I CCWWGG 3 cut(s) 219, 1011, 1043
Bst2BI CACGAG 1 cut(s) 1377
Bst2UI CCWGG 6 cut(s) 128, 191, 205, 606, 677, 801
Bst4CI ACNGT 6 cut(s) 14, 65, 262, 955, 961, 974
Bst6I CTCTTC 3 cut(s) 354, 777, 1125
BstACI GRCGYC 1 cut(s) 111
BstAUI TGTACA 1 cut(s) 908
BstBI TTCGAA 1 cut(s) 1071
BstC8I GCNNGC 5 cut(s) 124, 139, 1245, 1320, 1374
BstDEI CTNAG 6 cut(s) 290, 423, 513, 582, 660, 1402
BstDSI CCRYGG 2 cut(s) 261, 1043
BstEII GGTNACC 1 cut(s) 1038
BstF5I GGATG 3 cut(s) 970, 1153, 1348
BstFNI CGCG 3 cut(s) 137, 139, 141
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 3 cut(s) 113, 139, 141
BstKTI GATC 4 cut(s) 108, 145, 770, 834
BstMAI GTCTC 6 cut(s) 20, 180, 360, 452, 666, 845
BstMBI GATC 4 cut(s) 105, 142, 767, 831
BstMWI GCNNNNNNNGC 2 cut(s) 119, 740
BstNI CCWGG 6 cut(s) 128, 191, 205, 606, 677, 801
BstNSI RCATGY 2 cut(s) 817, 998
BstPAI GACNNNNGTC 1 cut(s) 263
BstPI GGTNACC 1 cut(s) 1038
BstSCI CCNGG 9 cut(s) 126, 189, 203, 324, 382, 604, 675, 799, 1338
BstSLI GKGCMC 1 cut(s) 1324
BstUI CGCG 3 cut(s) 137, 139, 141
BstV1I GCAGC 2 cut(s) 131, 1234
BstV2I GAAGAC 3 cut(s) 515, 991, 1389
BstX2I RGATCY 1 cut(s) 767
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 1 cut(s) 767
BsuRI GGCC 3 cut(s) 126, 159, 982
BtgI CCRYGG 2 cut(s) 261, 1043
BtsCI GGATG 3 cut(s) 970, 1153, 1348
BtsI GCAGTG 1 cut(s) 637
BtsIMutI CAGTG 2 cut(s) 10, 637
BveI ACCTGC 2 cut(s) 309, 1085
Cac8I GCNNGC 5 cut(s) 124, 139, 1245, 1320, 1374
CaiI CAGNNNCTG 1 cut(s) 1271
CfoI GCGC 3 cut(s) 113, 139, 141
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 4 cut(s) 258, 265, 294, 980
CsiI ACCWGGT 2 cut(s) 189, 675
Csp6I GTAC 6 cut(s) 280, 654, 909, 916, 1187, 1294
CspCI CAANNNNNGTGG 4 cut(s) 993, 1028, 1067, 1102
CviAII CATG 8 cut(s) 316, 618, 814, 887, 995, 1044, 1210, 1408
CviQI GTAC 6 cut(s) 280, 654, 909, 916, 1187, 1294
DdeI CTNAG 6 cut(s) 290, 423, 513, 582, 660, 1402
DinI GGCGCC 1 cut(s) 112
DpnI GATC 4 cut(s) 107, 144, 769, 833
DpnII GATC 4 cut(s) 105, 142, 767, 831
Eam1104I CTCTTC 3 cut(s) 354, 777, 1125
EarI CTCTTC 3 cut(s) 354, 777, 1125
Ecl136II GAGCTC 1 cut(s) 699
Eco130I CCWWGG 3 cut(s) 219, 1011, 1043
Eco24I GRGCYC 2 cut(s) 246, 701
Eco31I GGTCTC 3 cut(s) 180, 360, 666
Eco47I GGWCC 3 cut(s) 258, 265, 294
Eco53kI GAGCTC 1 cut(s) 699
Eco57I CTGAAG 2 cut(s) 1149, 1293
Eco88I CYCGRG 3 cut(s) 271, 370, 723
Eco91I GGTNACC 1 cut(s) 1038
EcoICRI GAGCTC 1 cut(s) 699
EcoO65I GGTNACC 1 cut(s) 1038
EcoRII CCWGG 6 cut(s) 126, 189, 203, 604, 675, 799
EcoT14I CCWWGG 3 cut(s) 219, 1011, 1043
EcoT38I GRGCYC 2 cut(s) 246, 701
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 3 cut(s) 219, 1011, 1043
Esp3I CGTCTC 1 cut(s) 452
FaeI CATG 8 cut(s) 319, 621, 817, 890, 998, 1047, 1213, 1411
FalI AAGNNNNNCTT 2 cut(s) 983, 1015
FaqI GGGAC 2 cut(s) 341, 740
FatI CATG 8 cut(s) 315, 617, 813, 886, 994, 1043, 1209, 1407
FauI CCCGC 1 cut(s) 98
FbaI TGATCA 1 cut(s) 831
FblI GTMKAC 2 cut(s) 210, 225
Fnu4HI GCNGC 4 cut(s) 57, 120, 135, 1248
FokI GGATG 3 cut(s) 977, 1160, 1355
FriOI GRGCYC 2 cut(s) 246, 701
Fsp4HI GCNGC 4 cut(s) 57, 120, 135, 1248
FspBI CTAG 2 cut(s) 705, 765
GlaI GCGC 3 cut(s) 112, 138, 140
GluI GCNGC 4 cut(s) 57, 120, 135, 1248
GsuI CTGGAG 2 cut(s) 756, 1248
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 3 cut(s) 126, 159, 982
HapII CCGG 6 cut(s) 114, 123, 148, 325, 383, 1339
HhaI GCGC 3 cut(s) 113, 139, 141
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 8 cut(s) 319, 621, 817, 890, 998, 1047, 1213, 1411
Hin6I GCGC 3 cut(s) 111, 137, 139
HinP1I GCGC 3 cut(s) 111, 137, 139
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 1176
HinfI GANTC 1 cut(s) 227
HpaII CCGG 6 cut(s) 114, 123, 148, 325, 383, 1339
HphI GGTGA 3 cut(s) 115, 517, 1226
Hpy166II GTNNAC 5 cut(s) 211, 226, 268, 656, 754
Hpy188I TCNGA 4 cut(s) 232, 1129, 1154, 1273
Hpy188III TCNNGA 7 cut(s) 292, 671, 725, 765, 776, 1379, 1390
Hpy8I GTNNAC 5 cut(s) 211, 226, 268, 656, 754
HpyAV CCTTC 5 cut(s) 149, 344, 369, 406, 1042
HpyCH4III ACNGT 6 cut(s) 14, 65, 262, 955, 961, 974
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 3 cut(s) 76, 761, 1372
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 740
HpyF3I CTNAG 6 cut(s) 290, 423, 513, 582, 660, 1402
HpySE526I ACGT 1 cut(s) 96
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 8 cut(s) 319, 621, 817, 890, 998, 1047, 1213, 1411
HspAI GCGC 3 cut(s) 111, 137, 139
KasI GGCGCC 1 cut(s) 110
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 831
Kzo9I GATC 4 cut(s) 105, 142, 767, 831
LmnI GCTCC 3 cut(s) 116, 696, 1021
Lsp1109I GCAGC 2 cut(s) 131, 1234
LweI GCATC 3 cut(s) 541, 770, 786
MabI ACCWGGT 2 cut(s) 189, 675
MaeI CTAG 2 cut(s) 705, 765
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 4 cut(s) 386, 955, 1020, 1038
MalI GATC 4 cut(s) 107, 144, 769, 833
MauBI CGCGCGCG 1 cut(s) 137
MboI GATC 4 cut(s) 105, 142, 767, 831
MfeI CAATTG 1 cut(s) 237
MflI RGATCY 1 cut(s) 767
MhlI GDGCHC 3 cut(s) 246, 701, 1324
MluCI AATT 6 cut(s) 237, 600, 633, 646, 1073, 1113
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 255
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 1 cut(s) 689
MspI CCGG 6 cut(s) 114, 123, 148, 325, 383, 1339
MspR9I CCNGG 9 cut(s) 128, 191, 205, 326, 384, 606, 677, 801, 1340
MunI CAATTG 1 cut(s) 237
Mva1269I GAATGC 1 cut(s) 1157
MvaI CCWGG 6 cut(s) 128, 191, 205, 606, 677, 801
MvnI CGCG 3 cut(s) 137, 139, 141
MwoI GCNNNNNNNGC 2 cut(s) 119, 740
NaeI GCCGGC 1 cut(s) 124
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 3 cut(s) 326, 384, 1340
NcoI CCATGG 1 cut(s) 1043
NdeII GATC 4 cut(s) 105, 142, 767, 831
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 8 cut(s) 319, 621, 817, 890, 998, 1047, 1213, 1411
NlaIV GGNNCC 6 cut(s) 112, 202, 323, 952, 981, 1017
NmeAIII GCCGAG 1 cut(s) 330
NmuCI GTSAC 3 cut(s) 386, 955, 1020
NspI RCATGY 2 cut(s) 817, 998
NspV TTCGAA 1 cut(s) 1071
PaeR7I CTCGAG 1 cut(s) 271
PauI GCGCGC 1 cut(s) 137
PciI ACATGT 1 cut(s) 813
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 1 cut(s) 1157
PdiI GCCGGC 1 cut(s) 124
PfoI TCCNGGA 1 cut(s) 324
PkrI GCNGC 4 cut(s) 58, 121, 136, 1249
PleI GAGTC 1 cut(s) 221
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 221
PscI ACATGT 1 cut(s) 813
PshAI GACNNNNGTC 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 701
Psp6I CCWGG 6 cut(s) 126, 189, 203, 604, 675, 799
PspEI GGTNACC 1 cut(s) 1038
PspGI CCWGG 6 cut(s) 126, 189, 203, 604, 675, 799
PspN4I GGNNCC 6 cut(s) 112, 202, 323, 952, 981, 1017
PspPI GGNCC 4 cut(s) 258, 265, 294, 980
PspXI VCTCGAGB 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 1271
PsuI RGATCY 1 cut(s) 767
PteI GCGCGC 1 cut(s) 137
RsaI GTAC 6 cut(s) 281, 655, 910, 917, 1188, 1295
RsaNI GTAC 6 cut(s) 280, 654, 909, 916, 1187, 1294
SacI GAGCTC 1 cut(s) 701
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 1 cut(s) 689
SatI GCNGC 4 cut(s) 57, 120, 135, 1248
Sau3AI GATC 4 cut(s) 105, 142, 767, 831
Sau96I GGNCC 4 cut(s) 258, 265, 294, 980
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 9 cut(s) 128, 191, 205, 326, 384, 606, 677, 801, 1340
SduI GDGCHC 3 cut(s) 246, 701, 1324
SexAI ACCWGGT 2 cut(s) 189, 675
SfaNI GCATC 3 cut(s) 541, 770, 786
SfoI GGCGCC 1 cut(s) 112
Sfr274I CTCGAG 1 cut(s) 271
SfuI TTCGAA 1 cut(s) 1071
SinI GGWCC 3 cut(s) 258, 265, 294
SlaI CTCGAG 1 cut(s) 271
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
Sse9I AATT 6 cut(s) 237, 600, 633, 646, 1073, 1113
SsiI CCGC 5 cut(s) 57, 80, 91, 101, 135
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 2 cut(s) 705, 765
SstI GAGCTC 1 cut(s) 701
StyD4I CCNGG 9 cut(s) 126, 189, 203, 324, 382, 604, 675, 799, 1338
StyI CCWWGG 3 cut(s) 219, 1011, 1043
TaaI ACNGT 6 cut(s) 14, 65, 262, 955, 961, 974
TaiI ACGT 1 cut(s) 99
TaqI TCGA 6 cut(s) 225, 246, 272, 670, 1071, 1111
TasI AATT 6 cut(s) 237, 600, 633, 646, 1073, 1113
TatI WGTACW 2 cut(s) 908, 1293
TauI GCSGC 2 cut(s) 59, 137
Tru1I TTAA 1 cut(s) 689
Tru9I TTAA 1 cut(s) 689
TscAI CASTG 2 cut(s) 17, 644
TseFI GTSAC 3 cut(s) 386, 955, 1020
TseI GCWGC 2 cut(s) 119, 1247
Tsp45I GTSAC 3 cut(s) 386, 955, 1020
TspDTI ATGAA 4 cut(s) 372, 795, 812, 861
TspGWI ACGGA 3 cut(s) 26, 476, 1008
TspRI CASTG 2 cut(s) 17, 644
VpaK11BI GGWCC 3 cut(s) 258, 265, 294
XapI RAATTY 2 cut(s) 600, 633
XbaI TCTAGA 1 cut(s) 764
XceI RCATGY 2 cut(s) 817, 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XhoI CTCGAG 1 cut(s) 271
XmiI GTMKAC 2 cut(s) 210, 225
XspI CTAG 2 cut(s) 705, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.