AT5G50120

cytosolic iron-sulfur protein assembly protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015017
Physical Location & Seq
Reverse (-)
20382310 .. 20384025
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50120.1

Sequence Viewer

Length: 1167 bp
ATGATACAATTTCAAGAATCCGAAGAAGAACCAGATTTTTCTCAAGAAGATCATCTAACATCTTCTTCTCAACCACTTCATCAATGTATTGCCACTCTTGTATGTCACACAGCATCCTACATTTCATCTCTAACTCTTGCAGGAAAGAGACTTTACACAGGCTCCAACGACGGGGTTGTTAGATTATGGAATGCAAACACGTTGGAAACATTAGCCGAAGCATCAAGCAACGGCGATGTAATAACAGGGGAGAGAGGAGGTGGAGGAGCAGTGAAATCTTTGGTAATCTTGGCTGATAAACTCTTTACAGCACACCAAGATCATAAGATACGTGTGTGGAAGATAAACGACGTCGTTGAAGAAGACGTGGGTGGTAAAAAGTACATGCATTTAGCGACGATGCCAACGATTAGCGATCGTTTTGCAAAGTGTTTGATGCCAAAGAACCAAGTTGAAATAAGGAGGCATAAGAAAGCTTCATGGGTTCACCATGTAGACGCGGTTTCGGGTTTAGCCTTATCAAGAGATGGAACACTACTCTACTCTGTCTCATGGGACAGGACGCTTAAAATCTGGCGAACCACCGATTTTAAATGCCTAGAGTCATTCACAAACGCCCACGATGATGCAATCAATGCGGTTGCATTATCCGAAAATGGTGATATATATACAGGATCTTCTGACCAAAGAATCAAAGTGTGGAGGAAGAACATCAATGAAGAGAATGTAAAGAAGAAGAGGAAACATTCTTTAGTTGCAATATTGTCAGAACATAATTCAGGTATTAACGCATTGGCATTGAGCGGTACTAATGGCTCTCTTTTGCATTCCGGTGGATCTGATGGATCGATATTGGTGTGGGAACGTGACGATGGTGGAGACATTGTAGTTGTTGGAATGCTTAGAGGTCACACAGAGTCTGTTCTATGCCTCGCGGTTGTTTCGGACATACTTTGTAGTGGCTCAGCAGATAAGACAGTGAGGTTGTGGAAATGTTCAGCCAAGGATTATTCATGCTTGGCTATGTTAGAGGGACATTTAGGACCGGTTAAGTGCTTGACAGGGGCATTTCGTGATTCGCGTAAAGCAGATGAGGCTTCTTATCATATTTATAGTGGAGGACTAGACTCTCAAGTTAAGGTTTGGCAGGTTTTGGTGCCGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

388

Amino Acids

42.5

Weight (kDa)

6.46

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 41 - 235 5.7e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 46 - 116 8.5e-06 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 47 - 203 5.5e-06 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 153 - 245 2.2e-07 CDC20/Fizzy WD40 domain
WDR55 PF24796 155 - 255 3.6e-10 WDR55
WD40_Gbeta PF25391 157 - 330 1.2e-12 G protein beta WD-40 repeat protein
WD40 PF00400 160 - 192 5.2e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 163 - 249 2.1e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 164 - 240 3.1e-09 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 164 - 235 7.6e-09 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 167 - 354 1.5e-11 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 168 - 291 7e-12 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 169 - 311 8e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 205 - 290 5.8e-06 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 222 - 337 3.8e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 247 - 384 2.5e-14 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 248 - 384 9.4e-17 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 252 - 383 2.7e-08 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 258 - 354 7.2e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 258 - 345 4.7e-11 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 270 - 385 5.5e-10 TEP-1 second beta-propeller
WD40 PF00400 298 - 330 9e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50120
fragaria_vesca FvH4_5g16381
malus_domestica MD06G1181200.v1.1 MD14G1187000.v1.1
prunus_persica Prupe.5G179200_v2.0.a1
pyrus_communis pycom06g16020
rosa_chinensis RchiOBHm_Chr7g0179711
rosa_laevigata RLG00000005328
rosa_multiflora Rmu_sc0005329.1_g000037
rosa_roxburghii Rroxscaffold_155G00443790 Rroxscaffold_3G00273530
rosa_samantha Rh7AG027000 Rh7BG026100 Rh7CG028100 Rh7DG027700
rosa_wichuraiana Rw7G002170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 1138
AccB1I GGYRCC 1 cut(s) 1156
AccBSI CCGCTC 1 cut(s) 804
AccI GTMKAC 1 cut(s) 495
AccII CGCG 3 cut(s) 500, 935, 1081
AciI CCGC 4 cut(s) 500, 638, 804, 935
AclWI GGATC 3 cut(s) 682, 844, 853
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 2 cut(s) 383, 808
AfiI CCNNNNNNNGG 1 cut(s) 171
AflIII ACRYGT 2 cut(s) 198, 331
AgeI ACCGGT 1 cut(s) 1045
AgsI TTSAA 3 cut(s) 14, 359, 455
AjiI CACGTC 1 cut(s) 367
AluBI AGCT 1 cut(s) 476
AluI AGCT 1 cut(s) 476
Alw26I GTCTC 3 cut(s) 142, 553, 873
AlwI GGATC 3 cut(s) 682, 844, 853
AlwNI CAGNNNCTG 1 cut(s) 920
AoxI GGCC 1 cut(s) 1161
AsiGI ACCGGT 1 cut(s) 1045
AspS9I GGNCC 1 cut(s) 1043
AsuHPI GGTGA 2 cut(s) 479, 671
AvaII GGWCC 1 cut(s) 1043
BaeI ACNNNNGTAYC 2 cut(s) 320, 353
BanI GGYRCC 1 cut(s) 1156
BbsI GAAGAC 1 cut(s) 369
BccI CCATC 3 cut(s) 521, 836, 866
BceAI ACGGC 1 cut(s) 247
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BcoDI GTCTC 3 cut(s) 142, 553, 873
BfaI CTAG 2 cut(s) 599, 1124
BfuAI ACCTGC 1 cut(s) 1138
BlpI GCTNAGC 1 cut(s) 964
Bme18I GGWCC 1 cut(s) 1043
BmgBI CACGTC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 1043
BmiI GGNNCC 2 cut(s) 163, 1158
BmsI GCATC 5 cut(s) 122, 230, 390, 426, 616
BpiI GAAGAC 1 cut(s) 369
Bpu1102I GCTNAGC 1 cut(s) 964
BpuEI CTTGAG 2 cut(s) 27, 1116
Bsa29I ATCGAT 1 cut(s) 848
BsaAI YACGTR 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 1002
BsaWI WCCGGW 2 cut(s) 830, 1045
BsaXI ACNNNNNCTCC 6 cut(s) 146, 176, 255, 285, 870, 900
Bsc4I CCNNNNNNNGG 1 cut(s) 171
Bse118I RCCGGY 2 cut(s) 1045, 1159
BseCI ATCGAT 1 cut(s) 848
BseDI CCNNGG 1 cut(s) 1002
BseGI GGATG 1 cut(s) 113
BseLI CCNNNNNNNGG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 978
BseRI GAGGAG 2 cut(s) 270, 279
Bsh1236I CGCG 3 cut(s) 500, 935, 1081
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 1 cut(s) 1163
BshNI GGYRCC 1 cut(s) 1156
BshTI ACCGGT 1 cut(s) 1045
BshVI ATCGAT 1 cut(s) 848
BsiEI CGRYCG 1 cut(s) 418
BsiSI CCGG 3 cut(s) 831, 1046, 1160
BslFI GGGAC 2 cut(s) 569, 1047
BslI CCNNNNNNNGG 1 cut(s) 171
BsmAI GTCTC 3 cut(s) 142, 553, 873
BsmFI GGGAC 2 cut(s) 569, 1047
BsmI GAATGC 3 cut(s) 196, 826, 903
BsnI GGCC 1 cut(s) 1163
Bsp143I GATC 6 cut(s) 49, 319, 415, 674, 836, 845
Bsp1720I GCTNAGC 1 cut(s) 964
BspACI CCGC 4 cut(s) 500, 638, 804, 935
BspANI GGCC 1 cut(s) 1163
BspCNI CTCAG 1 cut(s) 977
BspDI ATCGAT 1 cut(s) 848
BspFNI CGCG 3 cut(s) 500, 935, 1081
BspLI GGNNCC 2 cut(s) 163, 1158
BspMI ACCTGC 1 cut(s) 1138
BspPI GGATC 3 cut(s) 682, 844, 853
BspT107I GGYRCC 1 cut(s) 1156
BsrBI CCGCTC 1 cut(s) 804
BsrFI RCCGGY 2 cut(s) 1045, 1159
BssAI RCCGGY 2 cut(s) 1045, 1159
BssECI CCNNGG 1 cut(s) 1002
BssMI GATC 6 cut(s) 49, 319, 415, 674, 836, 845
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 1002
Bst4CI ACNGT 1 cut(s) 979
Bst6I CTCTTC 2 cut(s) 714, 731
BstACI GRCGYC 1 cut(s) 351
BstAPI GCANNNNNTGC 1 cut(s) 635
BstBAI YACGTR 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 1161
BstDEI CTNAG 2 cut(s) 902, 964
BstF5I GGATG 1 cut(s) 113
BstFNI CGCG 3 cut(s) 500, 935, 1081
BstKTI GATC 6 cut(s) 52, 322, 418, 677, 839, 848
BstMAI GTCTC 3 cut(s) 142, 553, 873
BstMBI GATC 6 cut(s) 49, 319, 415, 674, 836, 845
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 2 cut(s) 635, 1094
BstNSI RCATGY 1 cut(s) 388
BstUI CGCG 3 cut(s) 500, 935, 1081
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 2 cut(s) 674, 836
BstYI RGATCY 2 cut(s) 674, 836
Bsu15I ATCGAT 1 cut(s) 848
BsuRI GGCC 1 cut(s) 1163
BsuTUI ATCGAT 1 cut(s) 848
BtgZI GCGATG 1 cut(s) 249
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 1 cut(s) 113
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 2 cut(s) 276, 984
BveI ACCTGC 1 cut(s) 1138
Cac8I GCNNGC 1 cut(s) 1161
CaiI CAGNNNCTG 1 cut(s) 920
Cfr10I RCCGGY 2 cut(s) 1045, 1159
Cfr13I GGNCC 1 cut(s) 1043
ClaI ATCGAT 1 cut(s) 848
CseI GACGC 2 cut(s) 506, 571
Csp6I GTAC 2 cut(s) 382, 807
CspAI ACCGGT 1 cut(s) 1045
CviAII CATG 5 cut(s) 385, 480, 491, 552, 1014
CviQI GTAC 2 cut(s) 382, 807
DdeI CTNAG 2 cut(s) 902, 964
DpnI GATC 6 cut(s) 51, 321, 417, 676, 838, 847
DpnII GATC 6 cut(s) 49, 319, 415, 674, 836, 845
DraI TTTAAA 1 cut(s) 592
Eam1104I CTCTTC 2 cut(s) 714, 731
EarI CTCTTC 2 cut(s) 714, 731
Eco130I CCWWGG 1 cut(s) 1002
Eco47I GGWCC 1 cut(s) 1043
EcoT14I CCWWGG 1 cut(s) 1002
EcoT22I ATGCAT 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 1002
FaeI CATG 5 cut(s) 388, 483, 494, 555, 1017
FaqI GGGAC 2 cut(s) 569, 1047
FatI CATG 5 cut(s) 384, 479, 490, 551, 1013
FblI GTMKAC 1 cut(s) 495
FokI GGATG 1 cut(s) 100
FspBI CTAG 2 cut(s) 599, 1124
HaeIII GGCC 1 cut(s) 1163
HapII CCGG 3 cut(s) 831, 1046, 1160
HgaI GACGC 2 cut(s) 506, 571
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 5 cut(s) 388, 483, 494, 555, 1017
HindIII AAGCTT 1 cut(s) 474
HinfI GANTC 6 cut(s) 17, 602, 690, 917, 1076, 1127
HpaII CCGG 3 cut(s) 831, 1046, 1160
HphI GGTGA 2 cut(s) 479, 671
Hpy166II GTNNAC 2 cut(s) 487, 496
Hpy188I TCNGA 6 cut(s) 22, 652, 682, 769, 841, 946
Hpy188III TCNNGA 4 cut(s) 14, 44, 522, 1073
Hpy8I GTNNAC 2 cut(s) 487, 496
Hpy99I CGWCG 4 cut(s) 173, 353, 356, 400
HpyCH4III ACNGT 1 cut(s) 979
HpyCH4IV ACGT 5 cut(s) 200, 331, 351, 366, 865
HpyCH4V TGCA 8 cut(s) 140, 194, 388, 425, 629, 644, 758, 826
HpyF10VI GCNNNNNNNGC 2 cut(s) 635, 1094
HpyF3I CTNAG 2 cut(s) 902, 964
HpySE526I ACGT 5 cut(s) 200, 331, 351, 366, 865
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 5 cut(s) 388, 483, 494, 555, 1017
KroI GCCGGC 1 cut(s) 1159
KroNI GCCGGC 1 cut(s) 1161
Kzo9I GATC 6 cut(s) 49, 319, 415, 674, 836, 845
LmnI GCTCC 2 cut(s) 167, 266
LweI GCATC 5 cut(s) 122, 230, 390, 426, 616
MaeI CTAG 2 cut(s) 599, 1124
MaeII ACGT 5 cut(s) 200, 331, 351, 366, 865
MaeIII GTNAC 3 cut(s) 104, 866, 908
MalI GATC 6 cut(s) 51, 321, 417, 676, 838, 847
MbiI CCGCTC 1 cut(s) 804
MboI GATC 6 cut(s) 49, 319, 415, 674, 836, 845
MflI RGATCY 2 cut(s) 674, 836
MluCI AATT 2 cut(s) 8, 775
MlyI GAGTC 3 cut(s) 611, 926, 1121
MmeI TCCRAC 3 cut(s) 183, 189, 874
Mph1103I ATGCAT 1 cut(s) 390
MroNI GCCGGC 1 cut(s) 1159
MseI TTAA 5 cut(s) 567, 591, 786, 1050, 1137
MslI CAYNNNNRTG 2 cut(s) 624, 831
MspI CCGG 3 cut(s) 831, 1046, 1160
Mva1269I GAATGC 3 cut(s) 196, 826, 903
MvnI CGCG 3 cut(s) 500, 935, 1081
MwoI GCNNNNNNNGC 2 cut(s) 635, 1094
NaeI GCCGGC 1 cut(s) 1161
NdeII GATC 6 cut(s) 49, 319, 415, 674, 836, 845
NgoMIV GCCGGC 1 cut(s) 1159
NlaIII CATG 5 cut(s) 388, 483, 494, 555, 1017
NlaIV GGNNCC 2 cut(s) 163, 1158
NmuCI GTSAC 3 cut(s) 104, 866, 908
NsiI ATGCAT 1 cut(s) 390
NspI RCATGY 1 cut(s) 388
PcsI WCGNNNNNNNCGW 2 cut(s) 404, 1078
PctI GAATGC 3 cut(s) 196, 826, 903
PdiI GCCGGC 1 cut(s) 1161
PfeI GAWTC 3 cut(s) 17, 690, 1076
PinAI ACCGGT 1 cut(s) 1045
Ple19I CGATCG 1 cut(s) 418
PleI GAGTC 3 cut(s) 610, 925, 1121
PpsI GAGTC 3 cut(s) 610, 925, 1121
Ppu21I YACGTR 1 cut(s) 332
PspN4I GGNNCC 2 cut(s) 163, 1158
PspPI GGNCC 1 cut(s) 1043
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 2 cut(s) 674, 836
PvuI CGATCG 1 cut(s) 418
RsaI GTAC 2 cut(s) 383, 808
RsaNI GTAC 2 cut(s) 382, 807
RseI CAYNNNNRTG 2 cut(s) 624, 831
SaqAI TTAA 5 cut(s) 567, 591, 786, 1050, 1137
Sau3AI GATC 6 cut(s) 49, 319, 415, 674, 836, 845
Sau96I GGNCC 1 cut(s) 1043
SchI GAGTC 3 cut(s) 611, 926, 1121
SfaNI GCATC 5 cut(s) 122, 230, 390, 426, 616
SinI GGWCC 1 cut(s) 1043
SmiMI CAYNNNNRTG 2 cut(s) 624, 831
SmlI CTYRAG 2 cut(s) 42, 1131
SmoI CTYRAG 2 cut(s) 42, 1131
Sse9I AATT 2 cut(s) 8, 775
SsiI CCGC 4 cut(s) 500, 638, 804, 935
SspI AATATT 1 cut(s) 762
SspMI CTAG 2 cut(s) 599, 1124
StyI CCWWGG 1 cut(s) 1002
TaaI ACNGT 1 cut(s) 979
TaiI ACGT 5 cut(s) 203, 334, 354, 369, 868
TaqI TCGA 1 cut(s) 848
TasI AATT 2 cut(s) 8, 775
TatI WGTACW 1 cut(s) 381
TfiI GAWTC 3 cut(s) 17, 690, 1076
Tru1I TTAA 5 cut(s) 567, 591, 786, 1050, 1137
Tru9I TTAA 5 cut(s) 567, 591, 786, 1050, 1137
TscAI CASTG 2 cut(s) 276, 984
TseFI GTSAC 3 cut(s) 104, 866, 908
Tsp45I GTSAC 3 cut(s) 104, 866, 908
TspDTI ATGAA 5 cut(s) 68, 114, 468, 732, 1002
TspRI CASTG 2 cut(s) 276, 984
VpaK11BI GGWCC 1 cut(s) 1043
XceI RCATGY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 495
XspI CTAG 2 cut(s) 599, 1124
ZraI GACGTC 1 cut(s) 352
Zsp2I ATGCAT 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.