Prupe.5G179200_v2.0.a1

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0015017
Physical Location & Seq
Reverse (-)
15088103 .. 15089561
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G179200.1

Sequence Viewer

Length: 1206 bp
ATGGAATGCCACCAATCCTATCAGTATTATTCAAGCAGTGATCTTCATGACCAAGAAGATTTGTCTTACATTTCTTCTCAGCCAAGCCTCCAGTCAGTTCCTTCCCTTGCCTCACAATCCCACAATCAATCAAAACCCTGCCACCACTGCCTCACCACTTTGCAAGGCCAAAACTCCTACACATCCTCCCTCACCCTAGCTGGGAACTTCATCTACACGGGCTCTTCCGACGGAGAAATCAGGTCATGGAGGTGCAACTCTTTACACGAAGTCGACAGCGAAAACCCAACTCTTGATCACAACATGGTGGCTGCTGGAAAGGGTGCCGTGAAGTCCTTAGCAATCTCAGGTGACAAGCTCTTCAGTGCTCATCAAGACCACAAAATCAGAGTGTGGAAAATCAACACCCACCACGAGCAAGATCGCCAAAAGTGCTCGCGGCTAGCCACGCTTCCAACGCTTGGCGACCGCGCTGCGAAGCTCCTGATGCCCAATAACCATGTCCAAATAAGAAGGCACAAGAAGTGCACATGGGTTCATCATGTGGACACTGTGTCTGCCCTGGCCTTATCAAGTGATGAGCAGCTTCTGTACTCCGTTTCGTGGGATCGAACGCTCAAGGTTTGGAGCACAACAGACTTCAAATGCCTGGAGTCAGTAAGGAACGCGCACGATGATGCGATAAACGCAGTCACATCATCAAACGATGGAGATGTCTACACTGGATCAGCGGACAAAAGAATCAAGGTGTGGAGAAAAGACTTGGGAGAAAAGAAACATTCTTTAGTTGCCACATTAGAGAAGCACAATTCTGGGGTGAATGCGCTTGCTCTGAGCAGAGATGGCTGTGTGTTGTTTTCGGGGGCATGCGACAGGTCCATTCTGGTGTGGGAGAAGAAGGAAGGTGAGGCTCACATGGAGGTTGTGGGTGCGCTTAGAGGGCACACAAAGTCAATTTTGTGCTTGGCTGTGGTTTCTGACTTAGTCTGCAGTGGCTCCGCAGACAAAAGCATAAGAATTTGGAGAGGGGTTGATAGAAGCTACACGTGCTTAGCAGTTTTGGAAGGGCATAATGGTCCTGTCAAGTGTTTAACTGCTGCAGTTGATGATGACCATTGCAGCTCATCTAACATGTCTTGTATTATTTACAGTGGTAGTTTGGACTCTGACATCAAAATCTGGCAGGTCTTTGTTCCTAGTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

402

Amino Acids

44.29

Weight (kDa)

6.73

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50120
fragaria_vesca FvH4_5g16381
malus_domestica MD06G1181200.v1.1 MD14G1187000.v1.1
prunus_persica Prupe.5G179200_v2.0.a1
pyrus_communis pycom06g16020
rosa_chinensis RchiOBHm_Chr7g0179711
rosa_laevigata RLG00000005328
rosa_multiflora Rmu_sc0005329.1_g000037
rosa_roxburghii Rroxscaffold_155G00443790 Rroxscaffold_3G00273530
rosa_samantha Rh7AG027000 Rh7BG026100 Rh7CG028100 Rh7DG027700
rosa_wichuraiana Rw7G002170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1174
AccB1I GGYRCC 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 461
AccI GTMKAC 2 cut(s) 273, 717
AccII CGCG 3 cut(s) 439, 471, 668
AciI CCGC 4 cut(s) 439, 469, 731, 999
AclWI GGATC 2 cut(s) 615, 733
AcsI RAATTY 1 cut(s) 1017
AcuI CTGAAG 1 cut(s) 346
AcvI CACGTG 1 cut(s) 1047
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 3 cut(s) 201, 461, 603
AflIII ACRYGT 2 cut(s) 1044, 1131
AgsI TTSAA 2 cut(s) 33, 643
AhdI GACNNNNNGTC 1 cut(s) 553
AjnI CCWGG 2 cut(s) 561, 648
AluBI AGCT 6 cut(s) 200, 358, 481, 586, 1041, 1122
AluI AGCT 6 cut(s) 200, 358, 481, 586, 1041, 1122
Alw21I GWGCWC 4 cut(s) 370, 437, 530, 632
Alw44I GTGCAC 1 cut(s) 526
AlwI GGATC 2 cut(s) 615, 733
AlwNI CAGNNNCTG 1 cut(s) 589
AoxI GGCC 2 cut(s) 166, 564
ApaLI GTGCAC 1 cut(s) 526
ApeKI GCWGC 5 cut(s) 311, 473, 583, 1097, 1119
ApoI RAATTY 1 cut(s) 1017
AspLEI GCGC 4 cut(s) 473, 670, 826, 934
AspS9I GGNCC 2 cut(s) 876, 1076
AsuHPI GGTGA 5 cut(s) 145, 184, 362, 829, 917
AsuNHI GCTAGC 1 cut(s) 442
AvaII GGWCC 2 cut(s) 876, 1076
BaeGI GKGCMC 2 cut(s) 530, 945
BanI GGYRCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 224
BauI CACGAG 1 cut(s) 413
BbrPI CACGTG 1 cut(s) 1047
Bbv12I GWGCWC 4 cut(s) 370, 437, 530, 632
BbvI GCAGC 5 cut(s) 298, 460, 595, 1084, 1131
BccI CCATC 2 cut(s) 701, 836
BceAI ACGGC 1 cut(s) 311
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BciT130I CCWGG 2 cut(s) 563, 650
BclI TGATCA 1 cut(s) 295
BfaI CTAG 3 cut(s) 197, 443, 1197
BfmI CTRYAG 2 cut(s) 988, 1098
BfuAI ACCTGC 1 cut(s) 1174
BisI GCNGC 6 cut(s) 312, 440, 474, 584, 1098, 1120
BlpI GCTNAGC 1 cut(s) 1051
BlsI GCNGC 6 cut(s) 313, 441, 475, 585, 1099, 1121
Bme1390I CCNGG 2 cut(s) 563, 650
Bme18I GGWCC 2 cut(s) 876, 1076
BmeRI GACNNNNNGTC 1 cut(s) 553
BmgT120I GGNCC 2 cut(s) 876, 1076
BmiI GGNNCC 2 cut(s) 325, 997
BmrFI CCNGG 2 cut(s) 563, 650
BmsI GCATC 2 cut(s) 477, 667
BmtI GCTAGC 1 cut(s) 446
BpmI CTGGAG 2 cut(s) 74, 671
Bpu10I CCTNAGC 1 cut(s) 337
Bpu1102I GCTNAGC 1 cut(s) 1051
BpuEI CTTGAG 1 cut(s) 602
BsaAI YACGTR 1 cut(s) 1047
BsaJI CCNNGG 1 cut(s) 561
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 461, 603
Bse1I ACTGG 2 cut(s) 91, 727
Bse3DI GCAATG 1 cut(s) 1114
BseBI CCWGG 2 cut(s) 563, 650
BseDI CCNNGG 1 cut(s) 561
BseGI GGATG 1 cut(s) 182
BseLI CCNNNNNNNGG 3 cut(s) 201, 461, 603
BseMI GCAATG 1 cut(s) 1114
BseMII CTCAG 3 cut(s) 92, 360, 824
BseNI ACTGG 2 cut(s) 91, 727
BseSI GKGCMC 2 cut(s) 530, 945
BseXI GCAGC 5 cut(s) 298, 460, 595, 1084, 1131
BseYI CCCAGC 1 cut(s) 200
Bsh1236I CGCG 3 cut(s) 439, 471, 668
Bsh1285I CGRYCG 1 cut(s) 469
BshFI GGCC 2 cut(s) 168, 566
BshNI GGYRCC 1 cut(s) 323
BsiEI CGRYCG 1 cut(s) 469
BsiHKAI GWGCWC 4 cut(s) 370, 437, 530, 632
BslI CCNNNNNNNGG 3 cut(s) 201, 461, 603
BsmI GAATGC 2 cut(s) 11, 826
BsnI GGCC 2 cut(s) 168, 566
Bsp1286I GDGCHC 6 cut(s) 224, 370, 437, 530, 632, 945
Bsp143I GATC 5 cut(s) 40, 295, 421, 607, 725
Bsp1720I GCTNAGC 1 cut(s) 1051
BspACI CCGC 4 cut(s) 439, 469, 731, 999
BspANI GGCC 2 cut(s) 168, 566
BspCNI CTCAG 3 cut(s) 91, 359, 825
BspFNI CGCG 3 cut(s) 439, 471, 668
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 325, 997
BspMAI CTGCAG 2 cut(s) 992, 1102
BspMI ACCTGC 1 cut(s) 1174
BspOI GCTAGC 1 cut(s) 446
BspPI GGATC 2 cut(s) 615, 733
BspQI GCTCTTC 2 cut(s) 229, 365
BspT107I GGYRCC 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 1114
BsrI ACTGG 2 cut(s) 91, 727
BssECI CCNNGG 1 cut(s) 561
BssMI GATC 5 cut(s) 40, 295, 421, 607, 725
BssSI CACGAG 1 cut(s) 413
Bst2BI CACGAG 1 cut(s) 413
Bst2UI CCWGG 2 cut(s) 563, 650
Bst4CI ACNGT 2 cut(s) 553, 1151
Bst6I CTCTTC 2 cut(s) 229, 365
BstBAI YACGTR 1 cut(s) 1047
BstC8I GCNNGC 4 cut(s) 437, 444, 828, 868
BstDEI CTNAG 7 cut(s) 78, 337, 346, 833, 935, 982, 1051
BstF5I GGATG 1 cut(s) 182
BstFNI CGCG 3 cut(s) 439, 471, 668
BstHHI GCGC 4 cut(s) 473, 670, 826, 934
BstKTI GATC 5 cut(s) 43, 298, 424, 610, 728
BstMBI GATC 5 cut(s) 40, 295, 421, 607, 725
BstMCI CGRYCG 1 cut(s) 469
BstMWI GCNNNNNNNGC 9 cut(s) 147, 432, 448, 457, 487, 686, 843, 940, 1047
BstNI CCWGG 2 cut(s) 563, 650
BstNSI RCATGY 2 cut(s) 870, 1135
BstSCI CCNGG 2 cut(s) 561, 648
BstSFI CTRYAG 2 cut(s) 988, 1098
BstSLI GKGCMC 2 cut(s) 530, 945
BstUI CGCG 3 cut(s) 439, 471, 668
BstV1I GCAGC 5 cut(s) 298, 460, 595, 1084, 1131
BsuRI GGCC 2 cut(s) 168, 566
BtsCI GGATG 1 cut(s) 182
BtsI GCAGTG 3 cut(s) 43, 145, 997
BtsIMutI CAGTG 7 cut(s) 43, 145, 370, 549, 720, 997, 1156
BveI ACCTGC 1 cut(s) 1174
Cac8I GCNNGC 4 cut(s) 437, 444, 828, 868
CaiI CAGNNNCTG 1 cut(s) 589
CciI TCATGA 1 cut(s) 46
CfoI GCGC 4 cut(s) 473, 670, 826, 934
Cfr13I GGNCC 2 cut(s) 876, 1076
Csp6I GTAC 1 cut(s) 592
CviAII CATG 9 cut(s) 47, 246, 304, 500, 531, 542, 867, 916, 1132
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 7 cut(s) 78, 337, 346, 833, 935, 982, 1051
DpnI GATC 5 cut(s) 42, 297, 423, 609, 727
DpnII GATC 5 cut(s) 40, 295, 421, 607, 725
DriI GACNNNNNGTC 1 cut(s) 553
Eam1104I CTCTTC 2 cut(s) 229, 365
Eam1105I GACNNNNNGTC 1 cut(s) 553
EarI CTCTTC 2 cut(s) 229, 365
Eco24I GRGCYC 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 876, 1076
Eco57I CTGAAG 1 cut(s) 346
Eco72I CACGTG 1 cut(s) 1047
EcoRII CCWGG 2 cut(s) 561, 648
EcoT38I GRGCYC 1 cut(s) 224
FaeI CATG 9 cut(s) 50, 249, 307, 503, 534, 545, 870, 919, 1135
FatI CATG 9 cut(s) 46, 245, 303, 499, 530, 541, 866, 915, 1131
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 2 cut(s) 273, 717
Fnu4HI GCNGC 6 cut(s) 312, 440, 474, 584, 1098, 1120
FokI GGATG 1 cut(s) 169
FriOI GRGCYC 1 cut(s) 224
Fsp4HI GCNGC 6 cut(s) 312, 440, 474, 584, 1098, 1120
FspBI CTAG 3 cut(s) 197, 443, 1197
GlaI GCGC 4 cut(s) 472, 669, 825, 933
GluI GCNGC 6 cut(s) 312, 440, 474, 584, 1098, 1120
GsaI CCCAGC 1 cut(s) 204
GsuI CTGGAG 2 cut(s) 74, 671
HaeIII GGCC 2 cut(s) 168, 566
HhaI GCGC 4 cut(s) 473, 670, 826, 934
Hin1II CATG 9 cut(s) 50, 249, 307, 503, 534, 545, 870, 919, 1135
Hin6I GCGC 4 cut(s) 471, 668, 824, 932
HinP1I GCGC 4 cut(s) 471, 668, 824, 932
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 3 cut(s) 653, 741, 1163
HphI GGTGA 5 cut(s) 145, 184, 362, 829, 917
Hpy166II GTNNAC 4 cut(s) 274, 528, 547, 718
Hpy188I TCNGA 5 cut(s) 229, 389, 834, 979, 1168
Hpy188III TCNNGA 4 cut(s) 47, 293, 374, 484
Hpy8I GTNNAC 4 cut(s) 274, 528, 547, 718
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 5 cut(s) 111, 507, 892, 896, 1058
HpyCH4III ACNGT 2 cut(s) 553, 1151
HpyCH4IV ACGT 1 cut(s) 1046
HpyCH4V TGCA 6 cut(s) 163, 255, 528, 990, 1100, 1119
HpyF10VI GCNNNNNNNGC 9 cut(s) 147, 432, 448, 457, 487, 686, 843, 940, 1047
HpyF3I CTNAG 7 cut(s) 78, 337, 346, 833, 935, 982, 1051
HpySE526I ACGT 1 cut(s) 1046
Hsp92II CATG 9 cut(s) 50, 249, 307, 503, 534, 545, 870, 919, 1135
HspAI GCGC 4 cut(s) 471, 668, 824, 932
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 5 cut(s) 40, 295, 421, 607, 725
LguI GCTCTTC 2 cut(s) 229, 365
LmnI GCTCC 3 cut(s) 486, 627, 1001
Lsp1109I GCAGC 5 cut(s) 298, 460, 595, 1084, 1131
LweI GCATC 2 cut(s) 477, 667
MaeI CTAG 3 cut(s) 197, 443, 1197
MaeII ACGT 1 cut(s) 1046
MaeIII GTNAC 2 cut(s) 350, 691
MalI GATC 5 cut(s) 42, 297, 423, 609, 727
MboI GATC 5 cut(s) 40, 295, 421, 607, 725
MboII GAAGA 6 cut(s) 35, 66, 68, 216, 352, 907
MhlI GDGCHC 6 cut(s) 224, 370, 437, 530, 632, 945
MluCI AATT 3 cut(s) 808, 954, 1017
MlyI GAGTC 2 cut(s) 662, 1157
MmeI TCCRAC 2 cut(s) 252, 479
MseI TTAA 1 cut(s) 1091
MslI CAYNNNNRTG 3 cut(s) 250, 675, 884
MspA1I CMGCKG 1 cut(s) 731
MspR9I CCNGG 2 cut(s) 563, 650
Mva1269I GAATGC 2 cut(s) 11, 826
MvaI CCWGG 2 cut(s) 563, 650
MvnI CGCG 3 cut(s) 439, 471, 668
MwoI GCNNNNNNNGC 9 cut(s) 147, 432, 448, 457, 487, 686, 843, 940, 1047
NdeII GATC 5 cut(s) 40, 295, 421, 607, 725
NheI GCTAGC 1 cut(s) 442
NlaIII CATG 9 cut(s) 50, 249, 307, 503, 534, 545, 870, 919, 1135
NlaIV GGNNCC 2 cut(s) 325, 997
NmuCI GTSAC 2 cut(s) 350, 691
NspI RCATGY 2 cut(s) 870, 1135
PaeI GCATGC 1 cut(s) 870
PagI TCATGA 1 cut(s) 46
PciI ACATGT 1 cut(s) 1131
PciSI GCTCTTC 2 cut(s) 229, 365
PctI GAATGC 2 cut(s) 11, 826
PfeI GAWTC 1 cut(s) 741
PflFI GACNNNGTC 1 cut(s) 983
PflMI CCANNNNNTGG 1 cut(s) 461
PkrI GCNGC 6 cut(s) 313, 441, 475, 585, 1099, 1121
PleI GAGTC 2 cut(s) 661, 1157
PmaCI CACGTG 1 cut(s) 1047
PmlI CACGTG 1 cut(s) 1047
PpsI GAGTC 2 cut(s) 661, 1157
Ppu21I YACGTR 1 cut(s) 1047
PscI ACATGT 1 cut(s) 1131
Psp6I CCWGG 2 cut(s) 561, 648
PspCI CACGTG 1 cut(s) 1047
PspFI CCCAGC 1 cut(s) 200
PspGI CCWGG 2 cut(s) 561, 648
PspN4I GGNNCC 2 cut(s) 325, 997
PspPI GGNCC 2 cut(s) 876, 1076
PsrI GAACNNNNNNTAC 2 cut(s) 197, 229
PstI CTGCAG 2 cut(s) 992, 1102
PstNI CAGNNNCTG 1 cut(s) 589
PsyI GACNNNGTC 1 cut(s) 983
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 3 cut(s) 250, 675, 884
SalI GTCGAC 1 cut(s) 272
SapI GCTCTTC 2 cut(s) 229, 365
SaqAI TTAA 1 cut(s) 1091
SatI GCNGC 6 cut(s) 312, 440, 474, 584, 1098, 1120
Sau3AI GATC 5 cut(s) 40, 295, 421, 607, 725
Sau96I GGNCC 2 cut(s) 876, 1076
SchI GAGTC 2 cut(s) 662, 1157
ScrFI CCNGG 2 cut(s) 563, 650
SduI GDGCHC 6 cut(s) 224, 370, 437, 530, 632, 945
SfaNI GCATC 2 cut(s) 477, 667
SfcI CTRYAG 2 cut(s) 988, 1098
SinI GGWCC 2 cut(s) 876, 1076
SmiMI CAYNNNNRTG 3 cut(s) 250, 675, 884
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SphI GCATGC 1 cut(s) 870
Sse9I AATT 3 cut(s) 808, 954, 1017
SsiI CCGC 4 cut(s) 439, 469, 731, 999
SspMI CTAG 3 cut(s) 197, 443, 1197
StyD4I CCNGG 2 cut(s) 561, 648
TaaI ACNGT 2 cut(s) 553, 1151
TaiI ACGT 1 cut(s) 1049
TaqI TCGA 2 cut(s) 273, 610
TasI AATT 3 cut(s) 808, 954, 1017
TatI WGTACW 1 cut(s) 591
TauI GCSGC 1 cut(s) 442
TfiI GAWTC 1 cut(s) 741
Tru1I TTAA 1 cut(s) 1091
Tru9I TTAA 1 cut(s) 1091
TscAI CASTG 7 cut(s) 43, 152, 370, 556, 727, 997, 1156
TseFI GTSAC 2 cut(s) 350, 691
TseI GCWGC 5 cut(s) 311, 473, 583, 1097, 1119
Tsp45I GTSAC 2 cut(s) 350, 691
TspDTI ATGAA 3 cut(s) 35, 199, 527
TspGWI ACGGA 2 cut(s) 246, 586
TspRI CASTG 7 cut(s) 43, 152, 370, 556, 727, 997, 1156
Tth111I GACNNNGTC 1 cut(s) 983
Van91I CCANNNNNTGG 1 cut(s) 461
VneI GTGCAC 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 876, 1076
XapI RAATTY 1 cut(s) 1017
XceI RCATGY 2 cut(s) 870, 1135
XmiI GTMKAC 2 cut(s) 273, 717
XspI CTAG 3 cut(s) 197, 443, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.