MD14G1187000.v1.1

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015017
Physical Location & Seq
Reverse (-)
27934100 .. 27935314
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1187000.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGAATGCAACTCATCCTATCAGTACTCAGGCAATGATCTCTATGACCAATCAGAGTTTCCCTACCTTTCTTCTGAGCCAAGTCTCCAGTCAGTTCCTTCTCTCACCTCACAATCCCACAGGCAACACAGCTTTCCATCAAACAATTACCACCACTGCCTCACCACCTTGCAGGGCCACAACTCCTACATATCCTCTCTAACCCTAGCCGGAAACTTCATCTACACCGGCTCGTCTGACAGAGAAATCCGGTCGTGGAGGTGCAACCCTTTACTGGAATTCAACAACGAAAGCCCTACTCAGCATGATCGCAACATGGTGGCTGCAGGAAAGGGTGCAGTGAAGTCCCTAGCCATTTCAGGTGACAAGCTCTTCAGTGCTCATCAGGATCACAAAATCCGAGTGTGGAAAATCGACAGCCAGGAGCCTGATTTTCAAAAGTGCTCGCGGCTAGCTACGCTGCCAACGCTTGGTGACCGCGCCTCGAAGCTCCTGATGCCGAAGAACCATGTCCAAATAAGAAGGCACAAGAAGTGCACATGGGTGCATCATGTGGACGCAGTGTCTGCACTGGCCTTGTCGAGCGATGAGCTGCTGCTCTACTCCGTTTCGTGGGACCGTACACTCAAGGTTTGGAGCACAACAGACTTCAAATGCTTGGAGTCTGTGACAAATGCACATGATGATGCTATAAATGCAGTAACATCATCAAATGATGGACATGTTTACACTGGATCAGCAGACAAAAAAATTAAGGTTTGGAAAAAGGACTTTGGAGAGAAAAAGCACTTTTTAGTTGCTACACTTGAAAAGCACAATTCTGGGGTGAATGCACTAGCTATGAGCAGTGATGGGTGTGTGTTGTATTCTGGTGCATGCGACAGGTCCATTGTGGTTTGGGAGAAGAAAGATGCGGGTGGGAATATGGAAGTTCTGGGTGCGCTTAGAGGTCATACCAGGTCAATATTGTGCTTGGCTGTGGTGGCAGACTTGGTGTGTAGTGGCTCTGCAGATAAAAGTATAAGAATTTGGAGAGGGGTTAATAGAAGTTACTCATGTTTAGCAGTTTTGGAAGGGCATAAAGGTCCAGTGAAGTGTTTAACTGCTACAATTGATGACCATTGCAGCTCATCTGACTCCTCCTGTGTTATTTACAGTGGTGGTTTGGACTGTGACATCAAAGTCTGGCAGGTCTTTATTCCTAGCGAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

405

Amino Acids

44.68

Weight (kDa)

7.26

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 32 - 301 6.6e-06 SCAP Beta-propeller
WD40_WDHD1_1st PF24817 69 - 301 5.1e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 117 - 301 6.5e-12 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 118 - 375 2.1e-23 THOC3 beta-propeller domain
WDR55 PF24796 176 - 301 1.2e-09 WDR55
WD40_Gbeta PF25391 177 - 344 2.5e-12 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 179 - 304 4.2e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 182 - 255 7.6e-09 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 183 - 259 1.5e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 187 - 304 2.1e-16 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 189 - 325 5.1e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 225 - 398 2e-24 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 226 - 373 1.6e-06 WDHD1 first WD40 domain
Beta-prop_EML PF23409 228 - 306 3.4e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR75_1st PF23869 237 - 305 6.6e-08 WD repeat-containing protein 75 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 241 - 384 1.6e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 265 - 344 1.2e-10 WDR3 first beta-propeller domain
WD40 PF00400 266 - 300 3.7e-07 WD domain, G-beta repeat
EIF3I PF24805 269 - 345 2.6e-06 EIF3I
WD40_Prp19 PF24814 273 - 369 4.6e-12 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 278 - 400 7.1e-08 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 315 - 398 1e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50120
fragaria_vesca FvH4_5g16381
malus_domestica MD06G1181200.v1.1 MD14G1187000.v1.1
prunus_persica Prupe.5G179200_v2.0.a1
pyrus_communis pycom06g16020
rosa_chinensis RchiOBHm_Chr7g0179711
rosa_laevigata RLG00000005328
rosa_multiflora Rmu_sc0005329.1_g000037
rosa_roxburghii Rroxscaffold_155G00443790 Rroxscaffold_3G00273530
rosa_samantha Rh7AG027000 Rh7BG026100 Rh7CG028100 Rh7DG027700
rosa_wichuraiana Rw7G002170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1181
Acc36I ACCTGC 1 cut(s) 1180
AccB7I CCANNNNNTGG 1 cut(s) 470
AccII CGCG 2 cut(s) 448, 480
AciI CCGC 3 cut(s) 448, 478, 914
AclWI GGATC 2 cut(s) 396, 742
AcsI RAATTY 2 cut(s) 278, 1026
AcuI CTGAAG 1 cut(s) 358
AfaI GTAC 2 cut(s) 26, 622
AfiI CCNNNNNNNGG 3 cut(s) 274, 470, 612
AflIII ACRYGT 1 cut(s) 721
AgsI TTSAA 4 cut(s) 283, 437, 652, 809
AhdI GACNNNNNGTC 1 cut(s) 562
AjnI CCWGG 2 cut(s) 420, 956
AjuI GAANNNNNNNTTGG 2 cut(s) 42, 74
AleI CACNNNNGTG 1 cut(s) 542
AluBI AGCT 7 cut(s) 132, 370, 455, 490, 592, 839, 1128
AluI AGCT 7 cut(s) 132, 370, 455, 490, 592, 839, 1128
Alw21I GWGCWC 4 cut(s) 382, 446, 539, 641
Alw26I GTCTC 1 cut(s) 89
Alw44I GTGCAC 1 cut(s) 535
AlwI GGATC 2 cut(s) 396, 742
AlwNI CAGNNNCTG 1 cut(s) 566
AoxI GGCC 2 cut(s) 175, 573
ApaLI GTGCAC 1 cut(s) 535
ApeKI GCWGC 5 cut(s) 323, 460, 592, 595, 1125
ApoI RAATTY 2 cut(s) 278, 1026
AspLEI GCGC 2 cut(s) 482, 943
AspS9I GGNCC 4 cut(s) 175, 616, 885, 1085
AsuHPI GGTGA 5 cut(s) 97, 154, 374, 485, 838
AsuNHI GCTAGC 1 cut(s) 451
AvaII GGWCC 3 cut(s) 616, 885, 1085
BaeGI GKGCMC 1 cut(s) 539
Bbv12I GWGCWC 4 cut(s) 382, 446, 539, 641
BbvI GCAGC 5 cut(s) 310, 447, 579, 582, 1137
BccI CCATC 3 cut(s) 145, 710, 845
BciT130I CCWGG 2 cut(s) 422, 958
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 6 cut(s) 206, 350, 452, 836, 1203, 1213
BfmI CTRYAG 2 cut(s) 324, 1008
BfuAI ACCTGC 1 cut(s) 1180
BisI GCNGC 6 cut(s) 324, 449, 461, 593, 596, 1126
BlsI GCNGC 6 cut(s) 325, 450, 462, 594, 597, 1127
BmcAI AGTACT 1 cut(s) 26
Bme1390I CCNGG 2 cut(s) 422, 958
Bme18I GGWCC 3 cut(s) 616, 885, 1085
BmeRI GACNNNNNGTC 1 cut(s) 562
BmgT120I GGNCC 4 cut(s) 175, 616, 885, 1085
BmiI GGNNCC 2 cut(s) 426, 617
BmrFI CCNGG 2 cut(s) 422, 958
BmsI GCATC 4 cut(s) 486, 556, 676, 901
BmtI GCTAGC 1 cut(s) 455
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 611
BsaWI WCCGGW 1 cut(s) 249
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 470, 612
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 5 cut(s) 88, 279, 576, 736, 1088
Bse3DI GCAATG 2 cut(s) 40, 1120
BseBI CCWGG 2 cut(s) 422, 958
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 3 cut(s) 274, 470, 612
BseMI GCAATG 2 cut(s) 40, 1120
BseMII CTCAG 3 cut(s) 42, 66, 314
BseNI ACTGG 5 cut(s) 88, 279, 576, 736, 1088
BseRI GAGGAG 1 cut(s) 1129
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 5 cut(s) 310, 447, 579, 582, 1137
BsgI GTGCAG 2 cut(s) 357, 552
Bsh1236I CGCG 2 cut(s) 448, 480
Bsh1285I CGRYCG 1 cut(s) 254
BshFI GGCC 2 cut(s) 177, 575
BsiEI CGRYCG 1 cut(s) 254
BsiHKAI GWGCWC 4 cut(s) 382, 446, 539, 641
BsiSI CCGG 3 cut(s) 210, 228, 250
BslFI GGGAC 2 cut(s) 331, 629
BslI CCNNNNNNNGG 3 cut(s) 274, 470, 612
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 331, 629
BsmI GAATGC 2 cut(s) 11, 835
BsnI GGCC 2 cut(s) 177, 575
Bsp1286I GDGCHC 4 cut(s) 382, 446, 539, 641
Bsp143I GATC 4 cut(s) 37, 307, 388, 734
BspACI CCGC 3 cut(s) 448, 478, 914
BspANI GGCC 2 cut(s) 177, 575
BspCNI CTCAG 3 cut(s) 41, 67, 313
BspFNI CGCG 2 cut(s) 448, 480
BspLI GGNNCC 2 cut(s) 426, 617
BspMAI CTGCAG 2 cut(s) 328, 1012
BspMI ACCTGC 1 cut(s) 1180
BspOI GCTAGC 1 cut(s) 455
BspPI GGATC 2 cut(s) 396, 742
BspQI GCTCTTC 1 cut(s) 377
BsrDI GCAATG 2 cut(s) 40, 1120
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 5 cut(s) 88, 279, 576, 736, 1088
BssAI RCCGGY 1 cut(s) 227
BssMI GATC 4 cut(s) 37, 307, 388, 734
Bst2UI CCWGG 2 cut(s) 422, 958
Bst4CI ACNGT 3 cut(s) 620, 1157, 1172
Bst6I CTCTTC 1 cut(s) 377
BstAPI GCANNNNNTGC 1 cut(s) 566
BstC8I GCNNGC 3 cut(s) 446, 453, 877
BstDEI CTNAG 4 cut(s) 28, 75, 300, 944
BstEII GGTNACC 1 cut(s) 473
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 2 cut(s) 448, 480
BstHHI GCGC 2 cut(s) 482, 943
BstKTI GATC 4 cut(s) 40, 310, 391, 737
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 4 cut(s) 37, 307, 388, 734
BstMCI CGRYCG 1 cut(s) 254
BstMWI GCNNNNNNNGC 6 cut(s) 457, 466, 496, 566, 695, 983
BstNI CCWGG 2 cut(s) 422, 958
BstNSI RCATGY 2 cut(s) 725, 879
BstPI GGTNACC 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 420, 956
BstSFI CTRYAG 2 cut(s) 324, 1008
BstSLI GKGCMC 1 cut(s) 539
BstUI CGCG 2 cut(s) 448, 480
BstV1I GCAGC 5 cut(s) 310, 447, 579, 582, 1137
BsuRI GGCC 2 cut(s) 177, 575
BtgZI GCGATG 1 cut(s) 600
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 4 cut(s) 154, 345, 567, 853
BtsIMutI CAGTG 9 cut(s) 154, 345, 382, 567, 569, 729, 853, 1095, 1162
BveI ACCTGC 1 cut(s) 1180
Cac8I GCNNGC 3 cut(s) 446, 453, 877
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 2 cut(s) 482, 943
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 4 cut(s) 175, 616, 885, 1085
CseI GACGC 1 cut(s) 566
CsiI ACCWGGT 1 cut(s) 956
Csp6I GTAC 2 cut(s) 25, 621
CviAII CATG 9 cut(s) 305, 316, 509, 540, 551, 680, 722, 876, 1056
CviQI GTAC 2 cut(s) 25, 621
DdeI CTNAG 4 cut(s) 28, 75, 300, 944
DpnI GATC 4 cut(s) 39, 309, 390, 736
DpnII GATC 4 cut(s) 37, 307, 388, 734
DrdI GACNNNNNNGTC 1 cut(s) 1181
DriI GACNNNNNGTC 1 cut(s) 562
DseDI GACNNNNNNGTC 1 cut(s) 1181
Eam1104I CTCTTC 1 cut(s) 377
Eam1105I GACNNNNNGTC 1 cut(s) 562
EarI CTCTTC 1 cut(s) 377
Eco47I GGWCC 3 cut(s) 616, 885, 1085
Eco57I CTGAAG 1 cut(s) 358
Eco91I GGTNACC 1 cut(s) 473
EcoO65I GGTNACC 1 cut(s) 473
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 420, 956
FaeI CATG 9 cut(s) 308, 319, 512, 543, 554, 683, 725, 879, 1059
FaqI GGGAC 2 cut(s) 331, 629
FatI CATG 9 cut(s) 304, 315, 508, 539, 550, 679, 721, 875, 1055
FauI CCCGC 1 cut(s) 907
Fnu4HI GCNGC 6 cut(s) 324, 449, 461, 593, 596, 1126
Fsp4HI GCNGC 6 cut(s) 324, 449, 461, 593, 596, 1126
FspBI CTAG 6 cut(s) 206, 350, 452, 836, 1203, 1213
GlaI GCGC 2 cut(s) 481, 942
GluI GCNGC 6 cut(s) 324, 449, 461, 593, 596, 1126
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 2 cut(s) 177, 575
HapII CCGG 3 cut(s) 210, 228, 250
HgaI GACGC 1 cut(s) 566
HhaI GCGC 2 cut(s) 482, 943
Hin1II CATG 9 cut(s) 308, 319, 512, 543, 554, 683, 725, 879, 1059
Hin6I GCGC 2 cut(s) 480, 941
HinP1I GCGC 2 cut(s) 480, 941
HinfI GANTC 2 cut(s) 662, 1136
HpaII CCGG 3 cut(s) 210, 228, 250
HphI GGTGA 5 cut(s) 97, 154, 374, 485, 838
Hpy166II GTNNAC 4 cut(s) 537, 556, 623, 727
Hpy188I TCNGA 5 cut(s) 55, 76, 238, 401, 1135
Hpy188III TCNNGA 2 cut(s) 386, 493
Hpy8I GTNNAC 4 cut(s) 537, 556, 623, 727
HpyAV CCTTC 3 cut(s) 108, 516, 1067
HpyCH4III ACNGT 3 cut(s) 620, 1157, 1172
HpyF10VI GCNNNNNNNGC 6 cut(s) 457, 466, 496, 566, 695, 983
HpyF3I CTNAG 4 cut(s) 28, 75, 300, 944
Hsp92II CATG 9 cut(s) 308, 319, 512, 543, 554, 683, 725, 879, 1059
HspAI GCGC 2 cut(s) 480, 941
Kzo9I GATC 4 cut(s) 37, 307, 388, 734
LguI GCTCTTC 1 cut(s) 377
LmnI GCTCC 3 cut(s) 424, 495, 636
Lsp1109I GCAGC 5 cut(s) 310, 447, 579, 582, 1137
LweI GCATC 4 cut(s) 486, 556, 676, 901
MabI ACCWGGT 1 cut(s) 956
MaeI CTAG 6 cut(s) 206, 350, 452, 836, 1203, 1213
MaeIII GTNAC 6 cut(s) 362, 473, 667, 700, 1049, 1172
MalI GATC 4 cut(s) 39, 309, 390, 736
MboI GATC 4 cut(s) 37, 307, 388, 734
MboII GAAGA 4 cut(s) 63, 364, 514, 916
MfeI CAATTG 1 cut(s) 1110
MhlI GDGCHC 4 cut(s) 382, 446, 539, 641
MluCI AATT 6 cut(s) 145, 278, 750, 817, 1026, 1110
MlyI GAGTC 2 cut(s) 671, 1130
MnlI CCTC 8 cut(s) 118, 170, 205, 252, 493, 941, 1028, 1150
MseI TTAA 3 cut(s) 753, 1041, 1100
MslI CAYNNNNRTG 2 cut(s) 542, 684
MspI CCGG 3 cut(s) 210, 228, 250
MspR9I CCNGG 2 cut(s) 422, 958
MunI CAATTG 1 cut(s) 1110
Mva1269I GAATGC 2 cut(s) 11, 835
MvaI CCWGG 2 cut(s) 422, 958
MvnI CGCG 2 cut(s) 448, 480
MwoI GCNNNNNNNGC 6 cut(s) 457, 466, 496, 566, 695, 983
NdeII GATC 4 cut(s) 37, 307, 388, 734
NheI GCTAGC 1 cut(s) 451
NlaIII CATG 9 cut(s) 308, 319, 512, 543, 554, 683, 725, 879, 1059
NlaIV GGNNCC 2 cut(s) 426, 617
NmuCI GTSAC 4 cut(s) 362, 473, 667, 1172
NspI RCATGY 2 cut(s) 725, 879
OliI CACNNNNGTG 1 cut(s) 542
PaeI GCATGC 1 cut(s) 879
PciI ACATGT 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 377
PctI GAATGC 2 cut(s) 11, 835
PflMI CCANNNNNTGG 1 cut(s) 470
PkrI GCNGC 6 cut(s) 325, 450, 462, 594, 597, 1127
PleI GAGTC 2 cut(s) 670, 1130
PpsI GAGTC 2 cut(s) 670, 1130
PscI ACATGT 1 cut(s) 721
Psp6I CCWGG 2 cut(s) 420, 956
PspEI GGTNACC 1 cut(s) 473
PspGI CCWGG 2 cut(s) 420, 956
PspN4I GGNNCC 2 cut(s) 426, 617
PspPI GGNCC 4 cut(s) 175, 616, 885, 1085
PstI CTGCAG 2 cut(s) 328, 1012
PstNI CAGNNNCTG 1 cut(s) 566
RsaI GTAC 2 cut(s) 26, 622
RsaNI GTAC 2 cut(s) 25, 621
RseI CAYNNNNRTG 2 cut(s) 542, 684
SapI GCTCTTC 1 cut(s) 377
SaqAI TTAA 3 cut(s) 753, 1041, 1100
SatI GCNGC 6 cut(s) 324, 449, 461, 593, 596, 1126
Sau3AI GATC 4 cut(s) 37, 307, 388, 734
Sau96I GGNCC 4 cut(s) 175, 616, 885, 1085
ScaI AGTACT 1 cut(s) 26
SchI GAGTC 2 cut(s) 671, 1130
ScrFI CCNGG 2 cut(s) 422, 958
SduI GDGCHC 4 cut(s) 382, 446, 539, 641
SexAI ACCWGGT 1 cut(s) 956
SfaNI GCATC 4 cut(s) 486, 556, 676, 901
SfcI CTRYAG 2 cut(s) 324, 1008
SinI GGWCC 3 cut(s) 616, 885, 1085
SmiMI CAYNNNNRTG 2 cut(s) 542, 684
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
SphI GCATGC 1 cut(s) 879
Sse9I AATT 6 cut(s) 145, 278, 750, 817, 1026, 1110
SsiI CCGC 3 cut(s) 448, 478, 914
SspI AATATT 1 cut(s) 966
SspMI CTAG 6 cut(s) 206, 350, 452, 836, 1203, 1213
StyD4I CCNGG 2 cut(s) 420, 956
TaaI ACNGT 3 cut(s) 620, 1157, 1172
TaqI TCGA 3 cut(s) 414, 485, 581
TasI AATT 6 cut(s) 145, 278, 750, 817, 1026, 1110
TatI WGTACW 1 cut(s) 24
TauI GCSGC 1 cut(s) 451
Tru1I TTAA 3 cut(s) 753, 1041, 1100
Tru9I TTAA 3 cut(s) 753, 1041, 1100
TscAI CASTG 9 cut(s) 161, 345, 382, 567, 576, 736, 853, 1095, 1162
TseFI GTSAC 4 cut(s) 362, 473, 667, 1172
TseI GCWGC 5 cut(s) 323, 460, 592, 595, 1125
Tsp45I GTSAC 4 cut(s) 362, 473, 667, 1172
TspDTI ATGAA 1 cut(s) 208
TspGWI ACGGA 1 cut(s) 595
TspRI CASTG 9 cut(s) 161, 345, 382, 567, 576, 736, 853, 1095, 1162
Van91I CCANNNNNTGG 1 cut(s) 470
VneI GTGCAC 1 cut(s) 535
VpaK11BI GGWCC 3 cut(s) 616, 885, 1085
XapI RAATTY 2 cut(s) 278, 1026
XceI RCATGY 2 cut(s) 725, 879
XspI CTAG 6 cut(s) 206, 350, 452, 836, 1203, 1213
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.