FvH4_5g16381

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015017
Physical Location & Seq
Forward (+)
9326390 .. 9328224
1835 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16381.t1

Sequence Viewer

Length: 1227 bp
ATGGAATACCACCAGTCCTATCCGTACTCAAACAATGATTTTCAATCTCAGACAAGTCATCTTCATGGTGATCAATCAGATCATTACCTTACTTCTGTGCCAAGCCTCCAGTCAGTTCCTTCCCTGACCTCACAATCCCACAACCAAGAAAATCAGCCCACCTATCACCACTGCCTCACCACTTTGCAAGGCCACAACTCCTACATATCCTCTCTCACCATGTCTGGAAACTTCATCTATACCGGCTCTTCCGACAGAGAAATCCGGTCGTGGAAGTGCAACCCTTACGTCGAGTCCAATAACGAAAACTTGAGTGCTGATCATTGCATGGTGTCTGCTGGAAAGGGAGCAGTGAAGTCCCTAGCAATTTCAGGTGACAAGATCTTCAGTGCTCATCAAGATCACAAAATCCGAGTCTGGAAAATCGAAAACAATGAACCTGACCACCTAAGGTACTCGCGCTTAGCCACGTTGCCAACTCTTGGTGACCGCGCTGCTAAGCTTCTGATGCCGAAGCACCAGGTACAAATAAGAAGGCACAAGAAGTGCACTTGGATCCATCATGTGGACACAGTCTCTGCACTGGCCTTATCAAGGGATGAGTCACTTCTCTATTCTGTTTCATGGGATCGAACGCTCAAAGTATGGAGCACAACAGACTTCAAATGCTTGGAGTCAGTGAGAAATGCACATGATGATGCTATAAATGCAGTGGCAGTGTCATTGGAGGATGGACATGTTTATACTGGTTCAGCAGATAAAAAAATTAAGGTGTGGAAGAAAGAGTTAGGAGAAAAGAAACATTCTCTAATTGCTACATTAGAGAAGCACAATTCTGGGGTCAATGCACTTGCTCTGAGCAGTGATGGTTGTGTATTGTACTCTGGTGCATGTGACAGGTCCATTGTTGTTTGGGAGAAGATAGATAGTGAGGGTAACATGGGTGTTGTGGGTGCGCTAAGAGGTCATACCAAATCAATATTATGCTTAGCAGTGGTGTCAGACTTGGTGTGTAGTGGTTCTGCAGACCAAACCATAAGAATCTGGAGAGGGGTTGCTAGAAGTTATTCATGCTTGGCTATGTTAGAAGGGCATAAAGGTCCAGTCAAGTGTTTAACTGCTACAGTTGACCAGTGCACCACATCTGACACATCTAGTTATCTCATTTACAGCGGAGGTTTGGACTGTGACATCAAGGTTTGGCAGGTCTTTGTTCCTTGTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

409

Amino Acids

45.27

Weight (kDa)

7.06

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 73 - 227 5.9e-09 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 127 - 306 6.4e-06 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 162 - 266 5.4e-07 CDC20/Fizzy WD40 domain
WDR55 PF24796 180 - 259 3.8e-07 WDR55
WD40_Gbeta PF25391 181 - 349 1.7e-13 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 187 - 260 1.7e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 188 - 269 1.3e-09 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 189 - 307 4.5e-10 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 190 - 296 5.6e-06 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 191 - 268 1.5e-06 EIF3I
WD40_Prp19 PF24814 192 - 286 2.5e-09 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 192 - 306 6.2e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 192 - 309 4.6e-17 WDR3 second beta-propeller domain
NBCH_WD40 PF20426 193 - 262 9.6e-06 Neurobeachin beta propeller domain
Beta-prop_EML_2 PF23414 193 - 344 9.9e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR75_1st PF23869 226 - 308 2.6e-07 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 228 - 349 3.7e-06 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 228 - 402 2.2e-08 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 229 - 403 1.5e-25 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 232 - 403 6.1e-21 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 248 - 371 3.1e-09 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 265 - 377 9.3e-06 WDR55
WD40_CDC20-Fz PF24807 268 - 403 3.5e-08 CDC20/Fizzy WD40 domain
WD40 PF00400 270 - 305 2e-07 WD domain, G-beta repeat
EIF3I PF24805 274 - 350 8.4e-06 EIF3I
Beta-prop_THOC3 PF25174 277 - 369 1.4e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 278 - 373 2.4e-11 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 283 - 405 1.6e-08 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50120
fragaria_vesca FvH4_5g16381
malus_domestica MD06G1181200.v1.1 MD14G1187000.v1.1
prunus_persica Prupe.5G179200_v2.0.a1
pyrus_communis pycom06g16020
rosa_chinensis RchiOBHm_Chr7g0179711
rosa_laevigata RLG00000005328
rosa_multiflora Rmu_sc0005329.1_g000037
rosa_roxburghii Rroxscaffold_155G00443790 Rroxscaffold_3G00273530
rosa_samantha Rh7AG027000 Rh7BG026100 Rh7CG028100 Rh7DG027700
rosa_wichuraiana Rw7G002170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1195
AccB7I CCANNNNNTGG 2 cut(s) 482, 565
AccII CGCG 2 cut(s) 460, 492
AciI CCGC 2 cut(s) 490, 1173
AclWI GGATC 3 cut(s) 550, 563, 636
AcuI CTGAAG 1 cut(s) 370
AfaI GTAC 4 cut(s) 26, 455, 525, 881
AfiI CCNNNNNNNGG 3 cut(s) 482, 565, 594
AflIII ACRYGT 1 cut(s) 736
AgsI TTSAA 2 cut(s) 44, 664
AjnI CCWGG 1 cut(s) 519
AluBI AGCT 1 cut(s) 502
AluI AGCT 1 cut(s) 502
Alw21I GWGCWC 4 cut(s) 394, 551, 653, 1139
Alw26I GTCTC 1 cut(s) 580
Alw44I GTGCAC 2 cut(s) 547, 1135
AlwI GGATC 3 cut(s) 550, 563, 636
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 2 cut(s) 190, 585
ApaLI GTGCAC 2 cut(s) 547, 1135
ApeKI GCWGC 1 cut(s) 494
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
Asp700I GAANNNNTTC 1 cut(s) 1066
AspLEI GCGC 3 cut(s) 462, 494, 958
AspS9I GGNCC 2 cut(s) 900, 1100
AsuHPI GGTGA 6 cut(s) 80, 158, 169, 208, 386, 497
AvaII GGWCC 2 cut(s) 900, 1100
AxyI CCTNAGG 1 cut(s) 449
BaeGI GKGCMC 2 cut(s) 551, 1139
BamHI GGATCC 1 cut(s) 555
Bbv12I GWGCWC 4 cut(s) 394, 551, 653, 1139
BbvI GCAGC 1 cut(s) 481
BccI CCATC 3 cut(s) 567, 725, 860
BciT130I CCWGG 1 cut(s) 521
BclI TGATCA 2 cut(s) 70, 319
BcoDI GTCTC 1 cut(s) 580
BfaI CTAG 3 cut(s) 362, 1059, 1155
BfmI CTRYAG 2 cut(s) 1023, 1122
BfuAI ACCTGC 1 cut(s) 1195
BglII AGATCT 1 cut(s) 381
BisI GCNGC 1 cut(s) 495
BlpI GCTNAGC 3 cut(s) 463, 498, 988
BlsI GCNGC 1 cut(s) 496
Bme1390I CCNGG 1 cut(s) 521
Bme18I GGWCC 2 cut(s) 900, 1100
BmgT120I GGNCC 2 cut(s) 900, 1100
BmiI GGNNCC 1 cut(s) 557
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 2 cut(s) 498, 688
BpmI CTGGAG 2 cut(s) 92, 1066
Bpu1102I GCTNAGC 3 cut(s) 463, 498, 988
BpuEI CTTGAG 1 cut(s) 331
BsaWI WCCGGW 1 cut(s) 264
Bsc4I CCNNNNNNNGG 3 cut(s) 482, 565, 594
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 6 cut(s) 13, 109, 588, 751, 1103, 1132
Bse21I CCTNAGG 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 322
BseBI CCWGG 1 cut(s) 521
BseGI GGATG 2 cut(s) 604, 736
BseLI CCNNNNNNNGG 3 cut(s) 482, 565, 594
BseMI GCAATG 1 cut(s) 322
BseMII CTCAG 2 cut(s) 62, 848
BseNI ACTGG 6 cut(s) 13, 109, 588, 751, 1103, 1132
BseSI GKGCMC 2 cut(s) 551, 1139
BseXI GCAGC 1 cut(s) 481
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 2 cut(s) 460, 492
Bsh1285I CGRYCG 1 cut(s) 269
BshFI GGCC 2 cut(s) 192, 587
BsiEI CGRYCG 1 cut(s) 269
BsiHKAI GWGCWC 4 cut(s) 394, 551, 653, 1139
BsiSI CCGG 2 cut(s) 243, 265
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 3 cut(s) 482, 565, 594
BsmAI GTCTC 1 cut(s) 580
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 2 cut(s) 192, 587
Bsp1286I GDGCHC 4 cut(s) 394, 551, 653, 1139
Bsp143I GATC 7 cut(s) 70, 79, 319, 381, 400, 555, 628
Bsp1720I GCTNAGC 3 cut(s) 463, 498, 988
BspACI CCGC 2 cut(s) 490, 1173
BspANI GGCC 2 cut(s) 192, 587
BspCNI CTCAG 2 cut(s) 61, 849
BspFNI CGCG 2 cut(s) 460, 492
BspLI GGNNCC 1 cut(s) 557
BspMAI CTGCAG 1 cut(s) 1027
BspMI ACCTGC 1 cut(s) 1195
BspPI GGATC 3 cut(s) 550, 563, 636
BspQI GCTCTTC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 6 cut(s) 13, 109, 588, 751, 1103, 1132
BssAI RCCGGY 1 cut(s) 242
BssMI GATC 7 cut(s) 70, 79, 319, 381, 400, 555, 628
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 3 cut(s) 574, 1126, 1187
Bst6I CTCTTC 1 cut(s) 253
BstDEI CTNAG 7 cut(s) 48, 449, 463, 498, 857, 959, 988
BstEII GGTNACC 1 cut(s) 485
BstENI CCTNNNNNAGG 1 cut(s) 592
BstF5I GGATG 2 cut(s) 604, 736
BstFNI CGCG 2 cut(s) 460, 492
BstHHI GCGC 3 cut(s) 462, 494, 958
BstKTI GATC 7 cut(s) 73, 82, 322, 384, 403, 558, 631
BstMAI GTCTC 1 cut(s) 580
BstMBI GATC 7 cut(s) 70, 79, 319, 381, 400, 555, 628
BstMCI CGRYCG 1 cut(s) 269
BstMWI GCNNNNNNNGC 2 cut(s) 508, 707
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 2 cut(s) 740, 894
BstPI GGTNACC 1 cut(s) 485
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 2 cut(s) 1023, 1122
BstSLI GKGCMC 2 cut(s) 551, 1139
BstUI CGCG 2 cut(s) 460, 492
BstV1I GCAGC 1 cut(s) 481
BstX2I RGATCY 2 cut(s) 381, 555
BstYI RGATCY 2 cut(s) 381, 555
Bsu36I CCTNAGG 1 cut(s) 449
BsuRI GGCC 2 cut(s) 192, 587
BtsCI GGATG 2 cut(s) 604, 736
BtsI GCAGTG 6 cut(s) 169, 357, 717, 723, 868, 999
BveI ACCTGC 1 cut(s) 1195
CaiI CAGNNNCTG 1 cut(s) 578
CfoI GCGC 3 cut(s) 462, 494, 958
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 900, 1100
CsiI ACCWGGT 1 cut(s) 519
Csp6I GTAC 4 cut(s) 25, 454, 524, 880
CviJI RGCY 8 cut(s) 105, 157, 192, 246, 467, 502, 587, 1079
CviKI_1 RGCY 8 cut(s) 105, 157, 192, 246, 467, 502, 587, 1079
CviQI GTAC 4 cut(s) 25, 454, 524, 880
DdeI CTNAG 7 cut(s) 48, 449, 463, 498, 857, 959, 988
DpnI GATC 7 cut(s) 72, 81, 321, 383, 402, 557, 630
DpnII GATC 7 cut(s) 70, 79, 319, 381, 400, 555, 628
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 900, 1100
Eco57I CTGAAG 1 cut(s) 370
Eco81I CCTNAGG 1 cut(s) 449
Eco91I GGTNACC 1 cut(s) 485
EcoNI CCTNNNNNAGG 1 cut(s) 592
EcoO65I GGTNACC 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 519
FaqI GGGAC 1 cut(s) 343
FbaI TGATCA 2 cut(s) 70, 319
Fnu4HI GCNGC 1 cut(s) 495
FokI GGATG 2 cut(s) 611, 743
Fsp4HI GCNGC 1 cut(s) 495
FspBI CTAG 3 cut(s) 362, 1059, 1155
GlaI GCGC 3 cut(s) 461, 493, 957
GluI GCNGC 1 cut(s) 495
GsuI CTGGAG 2 cut(s) 92, 1066
HaeIII GGCC 2 cut(s) 192, 587
HapII CCGG 2 cut(s) 243, 265
HhaI GCGC 3 cut(s) 462, 494, 958
Hin6I GCGC 3 cut(s) 460, 492, 956
HinP1I GCGC 3 cut(s) 460, 492, 956
HincII GTYRAC 1 cut(s) 1129
HindII GTYRAC 1 cut(s) 1129
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 5 cut(s) 293, 414, 602, 674, 1041
HpaII CCGG 2 cut(s) 243, 265
HphI GGTGA 6 cut(s) 80, 158, 169, 208, 386, 497
Hpy166II GTNNAC 4 cut(s) 549, 568, 1129, 1137
Hpy188I TCNGA 8 cut(s) 51, 79, 253, 413, 507, 858, 1003, 1147
Hpy188III TCNNGA 4 cut(s) 225, 398, 418, 1045
Hpy8I GTNNAC 4 cut(s) 549, 568, 1129, 1137
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 3 cut(s) 129, 528, 1082
HpyCH4III ACNGT 3 cut(s) 574, 1126, 1187
HpyCH4IV ACGT 2 cut(s) 288, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 508, 707
HpyF3I CTNAG 7 cut(s) 48, 449, 463, 498, 857, 959, 988
HpySE526I ACGT 2 cut(s) 288, 470
HspAI GCGC 3 cut(s) 460, 492, 956
Ksp22I TGATCA 2 cut(s) 70, 319
Kzo9I GATC 7 cut(s) 70, 79, 319, 381, 400, 555, 628
LguI GCTCTTC 1 cut(s) 253
LmnI GCTCC 2 cut(s) 347, 648
Lsp1109I GCAGC 1 cut(s) 481
LweI GCATC 2 cut(s) 498, 688
MabI ACCWGGT 1 cut(s) 519
MaeI CTAG 3 cut(s) 362, 1059, 1155
MaeII ACGT 2 cut(s) 288, 470
MaeIII GTNAC 6 cut(s) 374, 485, 603, 893, 935, 1187
MalI GATC 7 cut(s) 72, 81, 321, 383, 402, 557, 630
MboI GATC 7 cut(s) 70, 79, 319, 381, 400, 555, 628
MboII GAAGA 5 cut(s) 53, 240, 376, 790, 931
MflI RGATCY 2 cut(s) 381, 555
MhlI GDGCHC 4 cut(s) 394, 551, 653, 1139
MluCI AATT 4 cut(s) 366, 765, 810, 832
MlyI GAGTC 4 cut(s) 302, 423, 611, 683
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 9 cut(s) 116, 139, 185, 220, 721, 925, 956, 1043, 1169
MroXI GAANNNNTTC 1 cut(s) 1066
MseI TTAA 2 cut(s) 768, 1115
MslI CAYNNNNRTG 2 cut(s) 63, 696
MspA1I CMGCKG 1 cut(s) 1173
MspI CCGG 2 cut(s) 243, 265
MspR9I CCNGG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 521
MvnI CGCG 2 cut(s) 460, 492
MwoI GCNNNNNNNGC 2 cut(s) 508, 707
NdeII GATC 7 cut(s) 70, 79, 319, 381, 400, 555, 628
NlaIV GGNNCC 1 cut(s) 557
NmuCI GTSAC 5 cut(s) 374, 485, 603, 893, 1187
NspI RCATGY 2 cut(s) 740, 894
PciI ACATGT 1 cut(s) 736
PciSI GCTCTTC 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 1066
PfeI GAWTC 1 cut(s) 1041
PflFI GACNNNGTC 1 cut(s) 572
PflMI CCANNNNNTGG 2 cut(s) 482, 565
PkrI GCNGC 1 cut(s) 496
PleI GAGTC 4 cut(s) 301, 422, 610, 682
PpsI GAGTC 4 cut(s) 301, 422, 610, 682
PscI ACATGT 1 cut(s) 736
Psp6I CCWGG 1 cut(s) 519
PspEI GGTNACC 1 cut(s) 485
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 557
PspPI GGNCC 2 cut(s) 900, 1100
PstI CTGCAG 1 cut(s) 1027
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 2 cut(s) 381, 555
PsyI GACNNNGTC 1 cut(s) 572
RsaI GTAC 4 cut(s) 26, 455, 525, 881
RsaNI GTAC 4 cut(s) 25, 454, 524, 880
RseI CAYNNNNRTG 2 cut(s) 63, 696
SapI GCTCTTC 1 cut(s) 253
SaqAI TTAA 2 cut(s) 768, 1115
SatI GCNGC 1 cut(s) 495
Sau3AI GATC 7 cut(s) 70, 79, 319, 381, 400, 555, 628
Sau96I GGNCC 2 cut(s) 900, 1100
SchI GAGTC 4 cut(s) 302, 423, 611, 683
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 4 cut(s) 394, 551, 653, 1139
SexAI ACCWGGT 1 cut(s) 519
SfaNI GCATC 2 cut(s) 498, 688
SfcI CTRYAG 2 cut(s) 1023, 1122
SinI GGWCC 2 cut(s) 900, 1100
SmiMI CAYNNNNRTG 2 cut(s) 63, 696
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
Sse9I AATT 4 cut(s) 366, 765, 810, 832
SsiI CCGC 2 cut(s) 490, 1173
SspI AATATT 1 cut(s) 981
SspMI CTAG 3 cut(s) 362, 1059, 1155
StyD4I CCNGG 1 cut(s) 519
TaaI ACNGT 3 cut(s) 574, 1126, 1187
TaiI ACGT 2 cut(s) 291, 473
TaqI TCGA 3 cut(s) 291, 426, 631
TasI AATT 4 cut(s) 366, 765, 810, 832
TatI WGTACW 1 cut(s) 879
TfiI GAWTC 1 cut(s) 1041
Tru1I TTAA 2 cut(s) 768, 1115
Tru9I TTAA 2 cut(s) 768, 1115
TseFI GTSAC 5 cut(s) 374, 485, 603, 893, 1187
TseI GCWGC 1 cut(s) 494
Tsp45I GTSAC 5 cut(s) 374, 485, 603, 893, 1187
TspDTI ATGAA 5 cut(s) 53, 223, 450, 612, 1059
TspGWI ACGGA 1 cut(s) 12
Tth111I GACNNNGTC 1 cut(s) 572
Van91I CCANNNNNTGG 2 cut(s) 482, 565
VneI GTGCAC 2 cut(s) 547, 1135
VpaK11BI GGWCC 2 cut(s) 900, 1100
XagI CCTNNNNNAGG 1 cut(s) 592
XceI RCATGY 2 cut(s) 740, 894
XmnI GAANNNNTTC 1 cut(s) 1066
XspI CTAG 3 cut(s) 362, 1059, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.