Rh7BG026100

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0015017
Physical Location & Seq
Reverse (-)
1853941 .. 1855164
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG026100.1

Sequence Viewer

Length: 1224 bp
ATGGAATACCACCAGACCTATCCGTACTCATACAATGATATTGAATCTCAGACAAATTATTTTCATGGTGATCAATCAGATCATTACCTTACTTCTGAGCCAAGCCTCCAGTCAGTTCCTTCCCTGTCCTCACAATCCCACAGTCAAGAAAATCAGCCCACCTATCACCATTGCCTCACCACTTTGCAAGGCCACAACTCCTACATATCCTCTCTCATCATATCTGGAAACTTCATCTATACCGGCTCCGACAGAGAAATCCGGTCGTGGAAGTGCAACCCTCACGTCGAGTCCTATAACGAAAACTTGAGTGCTGATCATTGCATGGTGTCTGCAGGAAAGGGAGCAGTGAAGTCCCTAGCAATTTCAGGTGACAAGCTCTTCAGTGCTCATCAAGATCACAAGATTCGAGTCTGGAAAATCGATAACAATGAACCTGACCACCAAAAGTACTCGCGCTTAGCCACGTTGCCAACGCTTGGTGACCGCGCTGCTAAGCTTCTGATGCCCAAGCACCAGGTCCAAATAAGAAGGCACAAGAAGAGCACTTGGGTCAATCATGTGGACACTGTCTCTGCACTTGCCTTATCAAGAGACGAGTCACTTCTTTATTCTGTTTCATGGGATCGAACGCTCAAGGTATGGAGCACATCAGACTTCAAATGTTTGGAGTCTGTGACAAATGCACATGATGATGCTATAAATGCAGTGGCAGTATCATTCGAGGATGGACATGTTTATACTGGTTCAGCAGACAAAAAAATTAAGGTGTGGAAGAAAGAGTTGGGAGAAAAGAAACATTCTCTAATTGCTATATTAGAGAAGCACAATTCTGGGGTCAATGCACTTGCTCTGAGCAGTGATGGTTGCGTGTTGTACTCCGGTGCATGTGACAGGTCCATTGTTGTTTGGGAGAAGATAGATAGTGAGGGTAACATGGGTGTTGTGGGTGCGCTTAGAGGTCATACCAAGTCCATATTATGCTTGGCAGTGGTGTCAGACTTGGTATGTAGTGGTTCTGCAGACCAAACGATAAGAATCTGGAGAGGGGTTGCTAGAAGTTATTCCTGTTTGGCCTTGTTGGAAGGGCATAAAGGTCCAGTCAAGTGTTTAACTGCTACAGTTGACCACTGCAACACATCTGACGCGTCCAATTTTCTCATTTACAGTGGGGGTTTGGACTGTGACATCAAGGTTTGGCAGGTCTTTGTTCCTTGTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

407

Amino Acids

45.19

Weight (kDa)

6.61

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 75 - 227 1.5e-06 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 126 - 305 4.3e-06 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 171 - 348 1.4e-13 CDC20/Fizzy WD40 domain
WDR55 PF24796 179 - 258 1.3e-06 WDR55
WD40_Gbeta PF25391 180 - 348 4.9e-14 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 183 - 269 1.1e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 184 - 237 2.1e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 186 - 259 9.6e-10 WDR3 first beta-propeller domain
EIF3I PF24805 190 - 267 1.4e-06 EIF3I
Beta-prop_WDR3_2nd PF25172 191 - 308 2.1e-16 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 191 - 305 3.4e-07 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 192 - 329 3.1e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 192 - 285 2.7e-09 Prp19 WD40 domain
WD40 PF00400 226 - 258 7e-06 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 227 - 348 5.7e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 228 - 402 9.5e-25 WDR5 beta-propeller domain
Beta-prop_WDR75_1st PF23869 230 - 307 3.8e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR3_1st PF25173 230 - 402 3.8e-20 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 232 - 306 5.4e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 235 - 349 4.4e-09 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 248 - 371 1.3e-08 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 268 - 402 1.7e-07 CDC20/Fizzy WD40 domain
WD40 PF00400 269 - 304 7.3e-07 WD domain, G-beta repeat
EIF3I PF24805 273 - 349 8e-06 EIF3I
Beta-prop_THOC3 PF25174 276 - 368 2.4e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 277 - 372 3.6e-11 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 282 - 402 1.3e-08 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50120
fragaria_vesca FvH4_5g16381
malus_domestica MD06G1181200.v1.1 MD14G1187000.v1.1
prunus_persica Prupe.5G179200_v2.0.a1
pyrus_communis pycom06g16020
rosa_chinensis RchiOBHm_Chr7g0179711
rosa_laevigata RLG00000005328
rosa_multiflora Rmu_sc0005329.1_g000037
rosa_roxburghii Rroxscaffold_155G00443790 Rroxscaffold_3G00273530
rosa_samantha Rh7AG027000 Rh7BG026100 Rh7CG028100 Rh7DG027700
rosa_wichuraiana Rw7G002170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1192
AccB7I CCANNNNNTGG 1 cut(s) 479
AccII CGCG 3 cut(s) 457, 489, 1148
AciI CCGC 1 cut(s) 487
AclWI GGATC 1 cut(s) 633
AcuI CTGAAG 1 cut(s) 367
AfaI GTAC 3 cut(s) 26, 452, 878
AfiI CCNNNNNNNGG 1 cut(s) 479
AflIII ACRYGT 2 cut(s) 733, 1146
AgsI TTSAA 2 cut(s) 44, 661
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 1 cut(s) 516
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AluBI AGCT 2 cut(s) 379, 499
AluI AGCT 2 cut(s) 379, 499
Alw21I GWGCWC 3 cut(s) 391, 548, 650
Alw26I GTCTC 2 cut(s) 577, 588
AlwI GGATC 1 cut(s) 633
AoxI GGCC 2 cut(s) 190, 1074
ApeKI GCWGC 1 cut(s) 491
Asp700I GAANNNNTTC 1 cut(s) 1063
AspLEI GCGC 3 cut(s) 459, 491, 955
AspS9I GGNCC 3 cut(s) 520, 897, 1097
AsuHPI GGTGA 5 cut(s) 80, 158, 169, 383, 494
AvaII GGWCC 3 cut(s) 520, 897, 1097
Bbv12I GWGCWC 3 cut(s) 391, 548, 650
BbvI GCAGC 1 cut(s) 478
BccI CCATC 2 cut(s) 722, 857
BciT130I CCWGG 1 cut(s) 518
BclI TGATCA 2 cut(s) 70, 316
BcoDI GTCTC 2 cut(s) 577, 588
BfaI CTAG 2 cut(s) 359, 1056
BfmI CTRYAG 3 cut(s) 333, 1020, 1119
BfuAI ACCTGC 1 cut(s) 1192
BisI GCNGC 1 cut(s) 492
BlpI GCTNAGC 2 cut(s) 460, 495
BlsI GCNGC 1 cut(s) 493
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 1 cut(s) 518
Bme18I GGWCC 3 cut(s) 520, 897, 1097
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 3 cut(s) 520, 897, 1097
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 1 cut(s) 518
BmsI GCATC 2 cut(s) 495, 685
BpmI CTGGAG 2 cut(s) 92, 1063
Bpu1102I GCTNAGC 2 cut(s) 460, 495
BpuEI CTTGAG 2 cut(s) 328, 620
Bsa29I ATCGAT 1 cut(s) 423
BsaBI GATNNNNATC 1 cut(s) 1037
BsaWI WCCGGW 2 cut(s) 261, 881
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 3 cut(s) 109, 748, 1100
Bse3DI GCAATG 2 cut(s) 169, 319
Bse8I GATNNNNATC 1 cut(s) 1037
BseBI CCWGG 1 cut(s) 518
BseCI ATCGAT 1 cut(s) 423
BseGI GGATG 1 cut(s) 733
BseJI GATNNNNATC 1 cut(s) 1037
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMI GCAATG 2 cut(s) 169, 319
BseMII CTCAG 3 cut(s) 62, 87, 845
BseNI ACTGG 3 cut(s) 109, 748, 1100
BseXI GCAGC 1 cut(s) 478
BsgI GTGCAG 1 cut(s) 561
Bsh1236I CGCG 3 cut(s) 457, 489, 1148
Bsh1285I CGRYCG 1 cut(s) 266
BshFI GGCC 2 cut(s) 192, 1076
BshVI ATCGAT 1 cut(s) 423
BsiEI CGRYCG 1 cut(s) 266
BsiHKAI GWGCWC 3 cut(s) 391, 548, 650
BsiSI CCGG 3 cut(s) 243, 262, 882
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 2 cut(s) 577, 588
BsmBI CGTCTC 1 cut(s) 588
BsmFI GGGAC 1 cut(s) 340
BsnI GGCC 2 cut(s) 192, 1076
Bsp1286I GDGCHC 3 cut(s) 391, 548, 650
Bsp143I GATC 5 cut(s) 70, 79, 316, 397, 625
Bsp1720I GCTNAGC 2 cut(s) 460, 495
BspACI CCGC 1 cut(s) 487
BspANI GGCC 2 cut(s) 192, 1076
BspCNI CTCAG 3 cut(s) 61, 88, 846
BspDI ATCGAT 1 cut(s) 423
BspFNI CGCG 3 cut(s) 457, 489, 1148
BspLI GGNNCC 1 cut(s) 247
BspMAI CTGCAG 2 cut(s) 337, 1024
BspMI ACCTGC 1 cut(s) 1192
BspPI GGATC 1 cut(s) 633
BspQI GCTCTTC 2 cut(s) 386, 536
BsrDI GCAATG 2 cut(s) 169, 319
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 3 cut(s) 109, 748, 1100
BssAI RCCGGY 1 cut(s) 242
BssMI GATC 5 cut(s) 70, 79, 316, 397, 625
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 5 cut(s) 143, 571, 1123, 1169, 1184
Bst6I CTCTTC 2 cut(s) 386, 536
BstDEI CTNAG 6 cut(s) 48, 96, 460, 495, 854, 956
BstEII GGTNACC 1 cut(s) 482
BstF5I GGATG 1 cut(s) 733
BstFNI CGCG 3 cut(s) 457, 489, 1148
BstHHI GCGC 3 cut(s) 459, 491, 955
BstKTI GATC 5 cut(s) 73, 82, 319, 400, 628
BstMAI GTCTC 2 cut(s) 577, 588
BstMBI GATC 5 cut(s) 70, 79, 316, 397, 625
BstMCI CGRYCG 1 cut(s) 266
BstMWI GCNNNNNNNGC 2 cut(s) 505, 704
BstNI CCWGG 1 cut(s) 518
BstNSI RCATGY 2 cut(s) 737, 891
BstPI GGTNACC 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 516
BstSFI CTRYAG 3 cut(s) 333, 1020, 1119
BstUI CGCG 3 cut(s) 457, 489, 1148
BstV1I GCAGC 1 cut(s) 478
Bsu15I ATCGAT 1 cut(s) 423
BsuRI GGCC 2 cut(s) 192, 1076
BsuTUI ATCGAT 1 cut(s) 423
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 1 cut(s) 733
BtsI GCAGTG 5 cut(s) 354, 714, 865, 996, 1129
BtsIMutI CAGTG 8 cut(s) 354, 391, 567, 714, 865, 996, 1129, 1174
BveI ACCTGC 1 cut(s) 1192
CfoI GCGC 3 cut(s) 459, 491, 955
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 3 cut(s) 520, 897, 1097
ClaI ATCGAT 1 cut(s) 423
CseI GACGC 2 cut(s) 1137, 1154
CsiI ACCWGGT 1 cut(s) 516
Csp6I GTAC 3 cut(s) 25, 451, 877
CviAII CATG 8 cut(s) 65, 325, 560, 621, 689, 734, 888, 937
CviJI RGCY 9 cut(s) 100, 105, 157, 192, 246, 379, 464, 499, 1076
CviKI_1 RGCY 9 cut(s) 100, 105, 157, 192, 246, 379, 464, 499, 1076
CviQI GTAC 3 cut(s) 25, 451, 877
DdeI CTNAG 6 cut(s) 48, 96, 460, 495, 854, 956
DpnI GATC 5 cut(s) 72, 81, 318, 399, 627
DpnII GATC 5 cut(s) 70, 79, 316, 397, 625
Eam1104I CTCTTC 2 cut(s) 386, 536
EarI CTCTTC 2 cut(s) 386, 536
Eco47I GGWCC 3 cut(s) 520, 897, 1097
Eco57I CTGAAG 1 cut(s) 367
Eco91I GGTNACC 1 cut(s) 482
EcoO65I GGTNACC 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 516
Esp3I CGTCTC 1 cut(s) 588
FaeI CATG 8 cut(s) 68, 328, 563, 624, 692, 737, 891, 940
FaqI GGGAC 1 cut(s) 340
FatI CATG 8 cut(s) 64, 324, 559, 620, 688, 733, 887, 936
FbaI TGATCA 2 cut(s) 70, 316
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 1 cut(s) 740
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 2 cut(s) 359, 1056
GlaI GCGC 3 cut(s) 458, 490, 954
GluI GCNGC 1 cut(s) 492
GsuI CTGGAG 2 cut(s) 92, 1063
HaeIII GGCC 2 cut(s) 192, 1076
HapII CCGG 3 cut(s) 243, 262, 882
HgaI GACGC 2 cut(s) 1137, 1154
HhaI GCGC 3 cut(s) 459, 491, 955
Hin1II CATG 8 cut(s) 68, 328, 563, 624, 692, 737, 891, 940
Hin6I GCGC 3 cut(s) 457, 489, 953
HinP1I GCGC 3 cut(s) 457, 489, 953
HincII GTYRAC 1 cut(s) 1126
HindII GTYRAC 1 cut(s) 1126
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 7 cut(s) 44, 290, 406, 411, 599, 671, 1038
HpaII CCGG 3 cut(s) 243, 262, 882
HphI GGTGA 5 cut(s) 80, 158, 169, 383, 494
Hpy166II GTNNAC 2 cut(s) 565, 1126
Hpy188I TCNGA 9 cut(s) 51, 79, 97, 250, 504, 655, 855, 1000, 1144
Hpy188III TCNNGA 6 cut(s) 146, 225, 395, 415, 591, 1042
Hpy8I GTNNAC 2 cut(s) 565, 1126
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 3 cut(s) 129, 525, 1079
HpyCH4III ACNGT 5 cut(s) 143, 571, 1123, 1169, 1184
HpyCH4IV ACGT 2 cut(s) 285, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 704
HpyF3I CTNAG 6 cut(s) 48, 96, 460, 495, 854, 956
HpySE526I ACGT 2 cut(s) 285, 467
Hsp92II CATG 8 cut(s) 68, 328, 563, 624, 692, 737, 891, 940
HspAI GCGC 3 cut(s) 457, 489, 953
Ksp22I TGATCA 2 cut(s) 70, 316
Kzo9I GATC 5 cut(s) 70, 79, 316, 397, 625
LguI GCTCTTC 2 cut(s) 386, 536
LmnI GCTCC 3 cut(s) 251, 344, 645
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 2 cut(s) 495, 685
MabI ACCWGGT 1 cut(s) 516
MaeI CTAG 2 cut(s) 359, 1056
MaeII ACGT 2 cut(s) 285, 467
MaeIII GTNAC 7 cut(s) 371, 482, 600, 676, 890, 932, 1184
MalI GATC 5 cut(s) 72, 81, 318, 399, 627
MboI GATC 5 cut(s) 70, 79, 316, 397, 625
MboII GAAGA 4 cut(s) 373, 553, 787, 928
MhlI GDGCHC 3 cut(s) 391, 548, 650
MluCI AATT 6 cut(s) 55, 363, 762, 807, 829, 1153
MluI ACGCGT 1 cut(s) 1146
MlyI GAGTC 4 cut(s) 299, 420, 608, 680
MmeI TCCRAC 2 cut(s) 273, 1062
MnlI CCTC 9 cut(s) 116, 139, 185, 220, 291, 718, 922, 953, 1040
MroXI GAANNNNTTC 1 cut(s) 1063
MseI TTAA 2 cut(s) 765, 1112
MslI CAYNNNNRTG 1 cut(s) 693
MspI CCGG 3 cut(s) 243, 262, 882
MspR9I CCNGG 1 cut(s) 518
MvaI CCWGG 1 cut(s) 518
MvnI CGCG 3 cut(s) 457, 489, 1148
MwoI GCNNNNNNNGC 2 cut(s) 505, 704
NdeII GATC 5 cut(s) 70, 79, 316, 397, 625
NlaIII CATG 8 cut(s) 68, 328, 563, 624, 692, 737, 891, 940
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 6 cut(s) 371, 482, 600, 676, 890, 1184
NspI RCATGY 2 cut(s) 737, 891
PciI ACATGT 1 cut(s) 733
PciSI GCTCTTC 2 cut(s) 386, 536
PdmI GAANNNNTTC 1 cut(s) 1063
PfeI GAWTC 3 cut(s) 44, 406, 1038
PflFI GACNNNGTC 1 cut(s) 569
PflMI CCANNNNNTGG 1 cut(s) 479
PkrI GCNGC 1 cut(s) 493
PleI GAGTC 4 cut(s) 298, 419, 607, 679
PpsI GAGTC 4 cut(s) 298, 419, 607, 679
PscI ACATGT 1 cut(s) 733
Psp6I CCWGG 1 cut(s) 516
PspEI GGTNACC 1 cut(s) 482
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 3 cut(s) 520, 897, 1097
PstI CTGCAG 2 cut(s) 337, 1024
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 3 cut(s) 26, 452, 878
RsaNI GTAC 3 cut(s) 25, 451, 877
RseI CAYNNNNRTG 1 cut(s) 693
SapI GCTCTTC 2 cut(s) 386, 536
SaqAI TTAA 2 cut(s) 765, 1112
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 5 cut(s) 70, 79, 316, 397, 625
Sau96I GGNCC 3 cut(s) 520, 897, 1097
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 4 cut(s) 299, 420, 608, 680
ScrFI CCNGG 1 cut(s) 518
SduI GDGCHC 3 cut(s) 391, 548, 650
SexAI ACCWGGT 1 cut(s) 516
SfaNI GCATC 2 cut(s) 495, 685
SfcI CTRYAG 3 cut(s) 333, 1020, 1119
SinI GGWCC 3 cut(s) 520, 897, 1097
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 2 cut(s) 307, 635
SmoI CTYRAG 2 cut(s) 307, 635
Sse9I AATT 6 cut(s) 55, 363, 762, 807, 829, 1153
SsiI CCGC 1 cut(s) 487
SspMI CTAG 2 cut(s) 359, 1056
StyD4I CCNGG 1 cut(s) 516
TaaI ACNGT 5 cut(s) 143, 571, 1123, 1169, 1184
TaiI ACGT 2 cut(s) 288, 470
TaqI TCGA 5 cut(s) 288, 409, 423, 628, 723
TasI AATT 6 cut(s) 55, 363, 762, 807, 829, 1153
TatI WGTACW 2 cut(s) 450, 876
TfiI GAWTC 3 cut(s) 44, 406, 1038
Tru1I TTAA 2 cut(s) 765, 1112
Tru9I TTAA 2 cut(s) 765, 1112
TscAI CASTG 8 cut(s) 354, 391, 574, 714, 865, 996, 1136, 1174
TseFI GTSAC 6 cut(s) 371, 482, 600, 676, 890, 1184
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 6 cut(s) 371, 482, 600, 676, 890, 1184
TspDTI ATGAA 4 cut(s) 53, 223, 447, 609
TspGWI ACGGA 1 cut(s) 12
TspRI CASTG 8 cut(s) 354, 391, 574, 714, 865, 996, 1136, 1174
Tth111I GACNNNGTC 1 cut(s) 569
Van91I CCANNNNNTGG 1 cut(s) 479
VpaK11BI GGWCC 3 cut(s) 520, 897, 1097
XceI RCATGY 2 cut(s) 737, 891
XcmI CCANNNNNNNNNTGG 1 cut(s) 982
XmnI GAANNNNTTC 1 cut(s) 1063
XspI CTAG 2 cut(s) 359, 1056
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.