MD06G1181200.v1.1

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015017
Physical Location & Seq
Reverse (-)
32053719 .. 32054918
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1181200.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGGAATGCTACTCGTCCGATCAGTACCCAGCCAATGACCTCTCTGACCAATCAGAGTTTCCCTACATTTCTCAGCCAAGTTTCCAGTCAGTTCCTTCACTCAACTCACAAAACAACTTTCCAACAAACACTTACCACCACTGTCTCACCACCTTGCAAGGCCACAACTCCTACATATCCTCTCTAACCCTAGCCGGAAACTTCATCTACACCGGCTTGTCCGATAGAGAAATCCGGTCGTGGAGGTGCAACCCTTTACTGGAATTCGACAACGAAAACACTACTCATCATGATCACAACATGGTGGCGGCAGGAAAGGGTGCAGTGAAGTCCCTGGCTATTTCGGGTGACAAGCTCTTCAGTGCTCATCAGGACCACAAGATTCGAGTGTGGAAAATCAACAGCCAGGAGCCCGATCTCCAAAAGTGCTCGCGGCTGGCCACACTGCCAACGCTTGGTGACCGTGCTTCGAAGCTCCTGATGCCGAAGAACCATGTCCAAATAAGAAGGCACAAGAAGTGCACTTGGGTGCATCATGTGGACACAGTGTCTGCCCTAGCCTTATCAAGCGATGAGCTGCTGCTGTACTCGGTTTCATGGGACCGTACACTCAAGGTTTGGAGCACAACAGACTTCAAATGCTTGGAGTCTGTGAGAAATGCACATGATGATGCTATAAACGCAGTAACATCGTCTAATGATGGATATGTTTACACTGGATCAGCAGACAAAAGAATTAAGGTTTGGAAAAAGGACTTCGGAGAGAAAAAGCACTTTTTAGTTGCTACACTGGAAAAGCACAATTCTGGAGTGAATGCACTAGCTCTGAGCAGTGATGGGTGTGTGTTGTATTCTGGTGCATGTGACAGGTCCATTGTGGTTTGGAAGAAGAAAGATGGGGGTGGGAATATGGAAGTTGTGGGTGTGCTTAGAGGTCATACCAAGTCAATATTGTGCTTGGCTGTGGTGGCAGACTTGGTGTGCAGTGGCTCTGCAGATAAAAGTATAAGAATTTGGAGAGGGGTTAATAAAATTTACTCATGTTTAGCAGTTTTGGAAGGGCATAAAGGTCCAGTTAAGTGTTTAACTGCTGCAATTGATGACCATTGCAGCTCATCTAACTCCTCCTGTGTTATTTACAGTGGTGGTTTGGACTGTGACATCAAAGTCTGGCAGGTCTTTGTTCCTAGTGACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

400

Amino Acids

44.13

Weight (kDa)

7.88

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 27 - 297 7.1e-06 SCAP Beta-propeller
WD40_WDHD1_1st PF24817 64 - 218 1.1e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 114 - 370 3.3e-23 THOC3 beta-propeller domain
WD40_Prp19 PF24814 115 - 295 1.3e-11 Prp19 WD40 domain
WDR55 PF24796 171 - 296 9.4e-10 WDR55
WD40_Gbeta PF25391 172 - 339 5.1e-13 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 174 - 298 5.5e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 177 - 250 1e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 178 - 254 1e-10 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 183 - 339 1.6e-09 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 183 - 299 1.3e-15 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 184 - 320 2.1e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML PF23409 203 - 275 8.1e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 219 - 365 1.2e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 220 - 393 2.4e-24 WDR5 beta-propeller domain
Beta-prop_EML PF23409 227 - 298 7.5e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR75_1st PF23869 229 - 298 1.5e-07 WD repeat-containing protein 75 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 238 - 364 7.7e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 257 - 393 3.5e-15 WDR3 first beta-propeller domain
WD40 PF00400 261 - 295 2.9e-07 WD domain, G-beta repeat
EIF3I PF24805 264 - 340 4.3e-06 EIF3I
WD40_Prp19 PF24814 268 - 364 1.8e-12 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 273 - 396 3.8e-08 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50120
fragaria_vesca FvH4_5g16381
malus_domestica MD06G1181200.v1.1 MD14G1187000.v1.1
prunus_persica Prupe.5G179200_v2.0.a1
pyrus_communis pycom06g16020
rosa_chinensis RchiOBHm_Chr7g0179711
rosa_laevigata RLG00000005328
rosa_multiflora Rmu_sc0005329.1_g000037
rosa_roxburghii Rroxscaffold_155G00443790 Rroxscaffold_3G00273530
rosa_samantha Rh7AG027000 Rh7BG026100 Rh7CG028100 Rh7DG027700
rosa_wichuraiana Rw7G002170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1166
Acc36I ACCTGC 1 cut(s) 1165
AccB7I CCANNNNNTGG 1 cut(s) 455
AccII CGCG 1 cut(s) 433
AciI CCGC 2 cut(s) 308, 433
AclWI GGATC 1 cut(s) 727
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 3 cut(s) 263, 1011, 1032
AcuI CTGAAG 1 cut(s) 343
AfaI GTAC 3 cut(s) 26, 587, 607
AfiI CCNNNNNNNGG 2 cut(s) 259, 455
AgsI TTSAA 1 cut(s) 637
AhdI GACNNNNNGTC 1 cut(s) 547
AjnI CCWGG 2 cut(s) 333, 405
AjuI GAANNNNNNNTTGG 4 cut(s) 42, 74, 727, 759
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 5 cut(s) 355, 475, 577, 824, 1113
AluI AGCT 5 cut(s) 355, 475, 577, 824, 1113
Alw21I GWGCWC 4 cut(s) 367, 431, 524, 626
Alw26I GTCTC 1 cut(s) 149
Alw44I GTGCAC 1 cut(s) 520
AlwI GGATC 1 cut(s) 727
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 2 cut(s) 160, 438
ApaLI GTGCAC 1 cut(s) 520
ApeKI GCWGC 4 cut(s) 577, 580, 1091, 1110
ApoI RAATTY 3 cut(s) 263, 1011, 1032
AspS9I GGNCC 4 cut(s) 373, 601, 870, 1070
AsuHPI GGTGA 3 cut(s) 139, 359, 470
AsuII TTCGAA 1 cut(s) 470
AvaII GGWCC 4 cut(s) 373, 601, 870, 1070
BaeGI GKGCMC 1 cut(s) 524
BalI TGGCCA 1 cut(s) 440
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 4 cut(s) 367, 431, 524, 626
BbvI GCAGC 4 cut(s) 564, 567, 1078, 1122
BccI CCATC 3 cut(s) 695, 830, 890
BcgI CGANNNNNNTGC 2 cut(s) 672, 706
BciT130I CCWGG 2 cut(s) 335, 407
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 4 cut(s) 191, 557, 821, 1188
BfmI CTRYAG 1 cut(s) 993
BfuAI ACCTGC 1 cut(s) 1165
BisI GCNGC 6 cut(s) 309, 434, 578, 581, 1092, 1111
BlsI GCNGC 6 cut(s) 310, 435, 579, 582, 1093, 1112
Bme1390I CCNGG 2 cut(s) 335, 407
Bme18I GGWCC 4 cut(s) 373, 601, 870, 1070
BmeRI GACNNNNNGTC 1 cut(s) 547
BmgT120I GGNCC 4 cut(s) 373, 601, 870, 1070
BmiI GGNNCC 2 cut(s) 411, 602
BmrFI CCNGG 2 cut(s) 335, 407
BmsI GCATC 3 cut(s) 471, 541, 661
BpmI CTGGAG 1 cut(s) 828
Bpu14I TTCGAA 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 234
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 455
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 5 cut(s) 85, 264, 721, 795, 1073
Bse3DI GCAATG 1 cut(s) 1105
BseBI CCWGG 2 cut(s) 335, 407
BseDI CCNNGG 1 cut(s) 333
BseLI CCNNNNNNNGG 2 cut(s) 259, 455
BseMI GCAATG 1 cut(s) 1105
BseMII CTCAG 2 cut(s) 86, 818
BseNI ACTGG 5 cut(s) 85, 264, 721, 795, 1073
BseRI GAGGAG 1 cut(s) 1114
BseSI GKGCMC 1 cut(s) 524
BseXI GCAGC 4 cut(s) 564, 567, 1078, 1122
BseYI CCCAGC 1 cut(s) 28
BsgI GTGCAG 2 cut(s) 342, 1003
Bsh1236I CGCG 1 cut(s) 433
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 2 cut(s) 162, 440
BsiEI CGRYCG 1 cut(s) 239
BsiHKAI GWGCWC 4 cut(s) 367, 431, 524, 626
BsiSI CCGG 3 cut(s) 195, 213, 235
BslFI GGGAC 2 cut(s) 316, 614
BslI CCNNNNNNNGG 2 cut(s) 259, 455
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 2 cut(s) 316, 614
BsmI GAATGC 2 cut(s) 11, 820
BsnI GGCC 2 cut(s) 162, 440
Bsp119I TTCGAA 1 cut(s) 470
Bsp1286I GDGCHC 5 cut(s) 367, 414, 431, 524, 626
Bsp143I GATC 4 cut(s) 19, 292, 415, 719
BspACI CCGC 2 cut(s) 308, 433
BspANI GGCC 2 cut(s) 162, 440
BspCNI CTCAG 2 cut(s) 85, 819
BspFNI CGCG 1 cut(s) 433
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 411, 602
BspMAI CTGCAG 1 cut(s) 997
BspMI ACCTGC 1 cut(s) 1165
BspPI GGATC 1 cut(s) 727
BspQI GCTCTTC 1 cut(s) 362
BspT104I TTCGAA 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 1105
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 5 cut(s) 85, 264, 721, 795, 1073
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 4 cut(s) 19, 292, 415, 719
Bst2UI CCWGG 2 cut(s) 335, 407
Bst4CI ACNGT 6 cut(s) 143, 464, 547, 605, 1142, 1157
Bst6I CTCTTC 1 cut(s) 362
BstBI TTCGAA 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 431, 438
BstDEI CTNAG 3 cut(s) 72, 827, 929
BstEII GGTNACC 1 cut(s) 458
BstFNI CGCG 1 cut(s) 433
BstKTI GATC 4 cut(s) 22, 295, 418, 722
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 4 cut(s) 19, 292, 415, 719
BstMCI CGRYCG 1 cut(s) 239
BstMWI GCNNNNNNNGC 3 cut(s) 481, 680, 968
BstNI CCWGG 2 cut(s) 335, 407
BstNSI RCATGY 1 cut(s) 864
BstPI GGTNACC 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 333, 405
BstSFI CTRYAG 1 cut(s) 993
BstSLI GKGCMC 1 cut(s) 524
BstUI CGCG 1 cut(s) 433
BstV1I GCAGC 4 cut(s) 564, 567, 1078, 1122
BsuRI GGCC 2 cut(s) 162, 440
BtgZI GCGATG 1 cut(s) 585
BtsI GCAGTG 4 cut(s) 330, 443, 838, 991
BveI ACCTGC 1 cut(s) 1165
Cac8I GCNNGC 2 cut(s) 431, 438
CaiI CAGNNNCTG 1 cut(s) 551
CciI TCATGA 1 cut(s) 289
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 4 cut(s) 373, 601, 870, 1070
Csp6I GTAC 3 cut(s) 25, 586, 606
CviAII CATG 8 cut(s) 290, 301, 494, 536, 597, 665, 861, 1041
CviQI GTAC 3 cut(s) 25, 586, 606
DdeI CTNAG 3 cut(s) 72, 827, 929
DpnI GATC 4 cut(s) 21, 294, 417, 721
DpnII GATC 4 cut(s) 19, 292, 415, 719
DrdI GACNNNNNNGTC 1 cut(s) 1166
DriI GACNNNNNGTC 1 cut(s) 547
DseDI GACNNNNNNGTC 1 cut(s) 1166
EaeI YGGCCR 1 cut(s) 438
Eam1104I CTCTTC 1 cut(s) 362
Eam1105I GACNNNNNGTC 1 cut(s) 547
EarI CTCTTC 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 4 cut(s) 373, 601, 870, 1070
Eco57I CTGAAG 1 cut(s) 343
Eco91I GGTNACC 1 cut(s) 458
EcoO65I GGTNACC 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 263
EcoRII CCWGG 2 cut(s) 333, 405
EcoT38I GRGCYC 1 cut(s) 414
FaeI CATG 8 cut(s) 293, 304, 497, 539, 600, 668, 864, 1044
FaqI GGGAC 2 cut(s) 316, 614
FatI CATG 8 cut(s) 289, 300, 493, 535, 596, 664, 860, 1040
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 6 cut(s) 309, 434, 578, 581, 1092, 1111
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 6 cut(s) 309, 434, 578, 581, 1092, 1111
FspBI CTAG 4 cut(s) 191, 557, 821, 1188
GluI GCNGC 6 cut(s) 309, 434, 578, 581, 1092, 1111
GsaI CCCAGC 1 cut(s) 32
GsuI CTGGAG 1 cut(s) 828
HaeIII GGCC 2 cut(s) 162, 440
HapII CCGG 3 cut(s) 195, 213, 235
Hin1II CATG 8 cut(s) 293, 304, 497, 539, 600, 668, 864, 1044
HinfI GANTC 2 cut(s) 382, 647
HpaII CCGG 3 cut(s) 195, 213, 235
HphI GGTGA 3 cut(s) 139, 359, 470
Hpy166II GTNNAC 4 cut(s) 522, 541, 608, 712
Hpy188I TCNGA 6 cut(s) 19, 46, 55, 223, 761, 828
Hpy188III TCNNGA 4 cut(s) 290, 371, 478, 807
Hpy8I GTNNAC 4 cut(s) 522, 541, 608, 712
HpyAV CCTTC 3 cut(s) 105, 501, 1052
HpyCH4III ACNGT 6 cut(s) 143, 464, 547, 605, 1142, 1157
HpyF10VI GCNNNNNNNGC 3 cut(s) 481, 680, 968
HpyF3I CTNAG 3 cut(s) 72, 827, 929
Hsp92II CATG 8 cut(s) 293, 304, 497, 539, 600, 668, 864, 1044
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 4 cut(s) 19, 292, 415, 719
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 3 cut(s) 409, 480, 621
Lsp1109I GCAGC 4 cut(s) 564, 567, 1078, 1122
LweI GCATC 3 cut(s) 471, 541, 661
MaeI CTAG 4 cut(s) 191, 557, 821, 1188
MaeIII GTNAC 6 cut(s) 347, 458, 685, 863, 1157, 1190
MalI GATC 4 cut(s) 21, 294, 417, 721
MboI GATC 4 cut(s) 19, 292, 415, 719
MboII GAAGA 4 cut(s) 349, 499, 898, 901
MfeI CAATTG 1 cut(s) 1095
MhlI GDGCHC 5 cut(s) 367, 414, 431, 524, 626
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 6 cut(s) 263, 735, 802, 1011, 1032, 1095
MluNI TGGCCA 1 cut(s) 440
MlyI GAGTC 1 cut(s) 656
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 6 cut(s) 50, 190, 237, 926, 1013, 1135
Mox20I TGGCCA 1 cut(s) 440
MscI TGGCCA 1 cut(s) 440
MseI TTAA 4 cut(s) 738, 1026, 1077, 1085
MslI CAYNNNNRTG 2 cut(s) 527, 669
Msp20I TGGCCA 1 cut(s) 440
MspI CCGG 3 cut(s) 195, 213, 235
MspR9I CCNGG 2 cut(s) 335, 407
MunI CAATTG 1 cut(s) 1095
Mva1269I GAATGC 2 cut(s) 11, 820
MvaI CCWGG 2 cut(s) 335, 407
MvnI CGCG 1 cut(s) 433
MwoI GCNNNNNNNGC 3 cut(s) 481, 680, 968
NdeII GATC 4 cut(s) 19, 292, 415, 719
NlaIII CATG 8 cut(s) 293, 304, 497, 539, 600, 668, 864, 1044
NlaIV GGNNCC 2 cut(s) 411, 602
NmuCI GTSAC 5 cut(s) 347, 458, 863, 1157, 1190
NspI RCATGY 1 cut(s) 864
NspV TTCGAA 1 cut(s) 470
OliI CACNNNNGTG 1 cut(s) 527
PagI TCATGA 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 362
PctI GAATGC 2 cut(s) 11, 820
PfeI GAWTC 1 cut(s) 382
PflMI CCANNNNNTGG 1 cut(s) 455
PkrI GCNGC 6 cut(s) 310, 435, 579, 582, 1093, 1112
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
Psp6I CCWGG 2 cut(s) 333, 405
PspEI GGTNACC 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 2 cut(s) 333, 405
PspN4I GGNNCC 2 cut(s) 411, 602
PspPI GGNCC 4 cut(s) 373, 601, 870, 1070
PstI CTGCAG 1 cut(s) 997
PstNI CAGNNNCTG 1 cut(s) 551
RsaI GTAC 3 cut(s) 26, 587, 607
RsaNI GTAC 3 cut(s) 25, 586, 606
RseI CAYNNNNRTG 2 cut(s) 527, 669
SapI GCTCTTC 1 cut(s) 362
SaqAI TTAA 4 cut(s) 738, 1026, 1077, 1085
SatI GCNGC 6 cut(s) 309, 434, 578, 581, 1092, 1111
Sau3AI GATC 4 cut(s) 19, 292, 415, 719
Sau96I GGNCC 4 cut(s) 373, 601, 870, 1070
SchI GAGTC 1 cut(s) 656
ScrFI CCNGG 2 cut(s) 335, 407
SduI GDGCHC 5 cut(s) 367, 414, 431, 524, 626
SfaNI GCATC 3 cut(s) 471, 541, 661
SfcI CTRYAG 1 cut(s) 993
SfuI TTCGAA 1 cut(s) 470
SinI GGWCC 4 cut(s) 373, 601, 870, 1070
SmiMI CAYNNNNRTG 2 cut(s) 527, 669
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 6 cut(s) 263, 735, 802, 1011, 1032, 1095
SsiI CCGC 2 cut(s) 308, 433
SspI AATATT 1 cut(s) 951
SspMI CTAG 4 cut(s) 191, 557, 821, 1188
StyD4I CCNGG 2 cut(s) 333, 405
TaaI ACNGT 6 cut(s) 143, 464, 547, 605, 1142, 1157
TaqI TCGA 3 cut(s) 267, 385, 470
TasI AATT 6 cut(s) 263, 735, 802, 1011, 1032, 1095
TatI WGTACW 1 cut(s) 585
TauI GCSGC 2 cut(s) 311, 436
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 4 cut(s) 738, 1026, 1077, 1085
Tru9I TTAA 4 cut(s) 738, 1026, 1077, 1085
TseFI GTSAC 5 cut(s) 347, 458, 863, 1157, 1190
TseI GCWGC 4 cut(s) 577, 580, 1091, 1110
Tsp45I GTSAC 5 cut(s) 347, 458, 863, 1157, 1190
TspDTI ATGAA 2 cut(s) 193, 585
Van91I CCANNNNNTGG 1 cut(s) 455
VneI GTGCAC 1 cut(s) 520
VpaK11BI GGWCC 4 cut(s) 373, 601, 870, 1070
XapI RAATTY 3 cut(s) 263, 1011, 1032
XceI RCATGY 1 cut(s) 864
XspI CTAG 4 cut(s) 191, 557, 821, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.