Rroxscaffold_155G00443790

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000155
OG0015017
Physical Location & Seq
Forward (+)
13666 .. 14892
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_155G00443790.1

Sequence Viewer

Length: 1227 bp
ATGGAATACCACCAGAACTATCCGTACTCATACAATGATATTGGATCTCAGACAAATTATTTTCATGGTGATCAATCAGATCATTACCTTACTTCTGAGCCAAGCCTCCAGTCAGTTCCTTCCCTGTCCTCACAATCCCGCAATCAAGAAGATCAGCCCACCTATCACCACTGCCTCACCACTTTGCAAGGCCACAACTCCTACATATCCTCTCTCACCATATCTGGAAACTTCATCTATACCGGCTCTTCCGACAGAGAAATCCGGTCATGGAAGTGCAACCCTCACGTCGAGTCCAATAACGAAAACTTGAGTGCTGATCATTGCATGGTGTCTGCAGGAAAGGGAGCAGTGAAGTCCCTAGCAATTTCAGGTGACAAGCTCTTCAGTGCTCATCAAGATCACAAGATTCGAGTCTGGAAAATTGATAACAATGAACCTGACCACCAAAAGTACTCGCGCTTAGCCACGTTGCCAACGCTTGGTGACCGCGCTGCTAAGCTTCTGATGCCCAAGCACCAGGTCCAAATAAGAAGGCACAAGAAGAGCACTTGGGTCAATCATGTGGACACTGTCTCTGCACTAGCCTTATCAAGAGACGAGTCACTTCTCTATTCTGTTTCATGGGATCGAACGCTCAAAGTATGGAGCACAACAGACTTCAAATGCTTGGAGTCTGTGACAAATGCACATGATGATGCTATAAATGCAGTGGCAGTATCATTCGAGGATGGACATGTTTATACTGGTTCAGCAGACAAAAAAATTAAGGTGTGGAAGAAAGAGTTGGGAGAAAAGAAACATTCTCTAATTGCTATATTAGAGAAGCACAATTCTGGGGTCAATGCACTTGCTCTGAGCAGTGATGGTTGCGTGTTGTACTCCGGTGCATGTGACAGGTCCATTGTTGTTTGGGAGAAGATAGATAGTGAGGGTAACATGGGTGTTGTGGGTGCGCTAAGAGGTCATACCAAGTCCATATTATGCTTGGCAGTGGTGTCAGACTTGGTATGTAGTGGTTCTGCAGACCAAACGATAAGAATCTGGAGAGGGGTTGCTAGAAGTTATTCCTGTTTGGCCTTGTTGGAAGGGCATAAAGGTCCAGTCAAGTGTTTAACTGCTACAGTTGACCACTGCAACACATCTGACGCGTCCAATTATCTCATTTACAGTGGCGGTTTGGACTGTGACATCAAGGTTTGGCAGGTCTTTGTTCCTTGTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

408

Amino Acids

45.23

Weight (kDa)

6.71

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 30 - 307 4e-06 SCAP Beta-propeller
WD40_CDC20-Fz PF24807 56 - 153 7.9e-06 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 74 - 228 4.5e-08 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 127 - 306 3.5e-06 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 162 - 266 2.3e-07 CDC20/Fizzy WD40 domain
WDR55 PF24796 180 - 259 1.5e-06 WDR55
WD40_Gbeta PF25391 181 - 349 4.5e-14 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 184 - 270 1.1e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 185 - 238 1.7e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 187 - 260 9.9e-10 WDR3 first beta-propeller domain
EIF3I PF24805 191 - 268 1.5e-06 EIF3I
Beta-prop_TEP1_2nd PF25047 192 - 307 2.7e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 192 - 309 1.8e-16 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 193 - 344 2.9e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 227 - 259 7e-06 WD domain, G-beta repeat
WD40_Prp19 PF24814 227 - 304 6.4e-08 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 228 - 349 5.7e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 229 - 403 8.1e-25 WDR5 beta-propeller domain
Beta-prop_EML PF23409 231 - 307 4.2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR75_1st PF23869 232 - 309 3.1e-06 WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st PF24817 234 - 350 5.5e-09 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 248 - 372 1.1e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 268 - 403 3.5e-15 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 269 - 403 1.3e-07 CDC20/Fizzy WD40 domain
WD40 PF00400 270 - 305 7.3e-07 WD domain, G-beta repeat
EIF3I PF24805 274 - 350 6.3e-06 EIF3I
Beta-prop_THOC3 PF25174 277 - 368 3e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 278 - 374 2.4e-11 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 283 - 405 2.2e-08 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50120
fragaria_vesca FvH4_5g16381
malus_domestica MD06G1181200.v1.1 MD14G1187000.v1.1
prunus_persica Prupe.5G179200_v2.0.a1
pyrus_communis pycom06g16020
rosa_chinensis RchiOBHm_Chr7g0179711
rosa_laevigata RLG00000005328
rosa_multiflora Rmu_sc0005329.1_g000037
rosa_roxburghii Rroxscaffold_155G00443790 Rroxscaffold_3G00273530
rosa_samantha Rh7AG027000 Rh7BG026100 Rh7CG028100 Rh7DG027700
rosa_wichuraiana Rw7G002170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1195
AccB7I CCANNNNNTGG 1 cut(s) 482
AccII CGCG 3 cut(s) 460, 492, 1151
AciI CCGC 3 cut(s) 139, 490, 1176
AclWI GGATC 2 cut(s) 52, 636
AcuI CTGAAG 1 cut(s) 370
AfaI GTAC 3 cut(s) 26, 455, 881
AfiI CCNNNNNNNGG 1 cut(s) 482
AflIII ACRYGT 2 cut(s) 736, 1149
AgsI TTSAA 1 cut(s) 664
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 1 cut(s) 519
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AluBI AGCT 2 cut(s) 382, 502
AluI AGCT 2 cut(s) 382, 502
Alw21I GWGCWC 3 cut(s) 394, 551, 653
Alw26I GTCTC 2 cut(s) 580, 591
AlwI GGATC 2 cut(s) 52, 636
AoxI GGCC 2 cut(s) 190, 1077
ApeKI GCWGC 1 cut(s) 494
Asp700I GAANNNNTTC 1 cut(s) 1066
AspLEI GCGC 3 cut(s) 462, 494, 958
AspS9I GGNCC 3 cut(s) 523, 900, 1100
AsuHPI GGTGA 6 cut(s) 80, 158, 169, 208, 386, 497
AvaII GGWCC 3 cut(s) 523, 900, 1100
Bbv12I GWGCWC 3 cut(s) 394, 551, 653
BbvI GCAGC 1 cut(s) 481
BccI CCATC 2 cut(s) 725, 860
BciT130I CCWGG 1 cut(s) 521
BclI TGATCA 2 cut(s) 70, 319
BcoDI GTCTC 2 cut(s) 580, 591
BfaI CTAG 3 cut(s) 362, 584, 1059
BfmI CTRYAG 3 cut(s) 336, 1023, 1122
BfuAI ACCTGC 1 cut(s) 1195
BisI GCNGC 1 cut(s) 495
BlpI GCTNAGC 2 cut(s) 463, 498
BlsI GCNGC 1 cut(s) 496
BmcAI AGTACT 1 cut(s) 455
Bme1390I CCNGG 1 cut(s) 521
Bme18I GGWCC 3 cut(s) 523, 900, 1100
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 3 cut(s) 523, 900, 1100
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 2 cut(s) 498, 688
BpmI CTGGAG 2 cut(s) 92, 1066
Bpu1102I GCTNAGC 2 cut(s) 463, 498
BpuEI CTTGAG 1 cut(s) 331
BsaBI GATNNNNATC 1 cut(s) 1040
BsaWI WCCGGW 2 cut(s) 264, 884
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 3 cut(s) 109, 751, 1103
Bse3DI GCAATG 1 cut(s) 322
Bse8I GATNNNNATC 1 cut(s) 1040
BseBI CCWGG 1 cut(s) 521
BseGI GGATG 1 cut(s) 736
BseJI GATNNNNATC 1 cut(s) 1040
BseLI CCNNNNNNNGG 1 cut(s) 482
BseMI GCAATG 1 cut(s) 322
BseMII CTCAG 3 cut(s) 62, 87, 848
BseNI ACTGG 3 cut(s) 109, 751, 1103
BseXI GCAGC 1 cut(s) 481
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 3 cut(s) 460, 492, 1151
BshFI GGCC 2 cut(s) 192, 1079
BsiHKAI GWGCWC 3 cut(s) 394, 551, 653
BsiSI CCGG 3 cut(s) 243, 265, 885
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 1 cut(s) 482
BsmAI GTCTC 2 cut(s) 580, 591
BsmBI CGTCTC 1 cut(s) 591
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 2 cut(s) 192, 1079
Bsp1286I GDGCHC 3 cut(s) 394, 551, 653
Bsp143I GATC 7 cut(s) 44, 70, 79, 151, 319, 400, 628
Bsp1720I GCTNAGC 2 cut(s) 463, 498
BspACI CCGC 3 cut(s) 139, 490, 1176
BspANI GGCC 2 cut(s) 192, 1079
BspCNI CTCAG 3 cut(s) 61, 88, 849
BspFNI CGCG 3 cut(s) 460, 492, 1151
BspMAI CTGCAG 2 cut(s) 340, 1027
BspMI ACCTGC 1 cut(s) 1195
BspPI GGATC 2 cut(s) 52, 636
BspQI GCTCTTC 3 cut(s) 253, 389, 539
BsrDI GCAATG 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 3 cut(s) 109, 751, 1103
BssAI RCCGGY 1 cut(s) 242
BssMI GATC 7 cut(s) 44, 70, 79, 151, 319, 400, 628
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 4 cut(s) 574, 1126, 1172, 1187
Bst6I CTCTTC 3 cut(s) 253, 389, 539
BstDEI CTNAG 6 cut(s) 48, 96, 463, 498, 857, 959
BstEII GGTNACC 1 cut(s) 485
BstF5I GGATG 1 cut(s) 736
BstFNI CGCG 3 cut(s) 460, 492, 1151
BstHHI GCGC 3 cut(s) 462, 494, 958
BstKTI GATC 7 cut(s) 47, 73, 82, 154, 322, 403, 631
BstMAI GTCTC 2 cut(s) 580, 591
BstMBI GATC 7 cut(s) 44, 70, 79, 151, 319, 400, 628
BstMWI GCNNNNNNNGC 2 cut(s) 508, 707
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 2 cut(s) 740, 894
BstPI GGTNACC 1 cut(s) 485
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 3 cut(s) 336, 1023, 1122
BstUI CGCG 3 cut(s) 460, 492, 1151
BstV1I GCAGC 1 cut(s) 481
BstX2I RGATCY 1 cut(s) 44
BstYI RGATCY 1 cut(s) 44
BsuRI GGCC 2 cut(s) 192, 1079
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 1 cut(s) 736
BtsI GCAGTG 6 cut(s) 169, 357, 717, 868, 999, 1132
BtsIMutI CAGTG 9 cut(s) 169, 357, 394, 570, 717, 868, 999, 1132, 1177
BveI ACCTGC 1 cut(s) 1195
CfoI GCGC 3 cut(s) 462, 494, 958
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 3 cut(s) 523, 900, 1100
CseI GACGC 2 cut(s) 1140, 1157
CsiI ACCWGGT 1 cut(s) 519
Csp6I GTAC 3 cut(s) 25, 454, 880
CviAII CATG 9 cut(s) 65, 270, 328, 563, 624, 692, 737, 891, 940
CviQI GTAC 3 cut(s) 25, 454, 880
DdeI CTNAG 6 cut(s) 48, 96, 463, 498, 857, 959
DpnI GATC 7 cut(s) 46, 72, 81, 153, 321, 402, 630
DpnII GATC 7 cut(s) 44, 70, 79, 151, 319, 400, 628
Eam1104I CTCTTC 3 cut(s) 253, 389, 539
EarI CTCTTC 3 cut(s) 253, 389, 539
Eco47I GGWCC 3 cut(s) 523, 900, 1100
Eco57I CTGAAG 1 cut(s) 370
Eco91I GGTNACC 1 cut(s) 485
EcoO65I GGTNACC 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 519
Esp3I CGTCTC 1 cut(s) 591
FaeI CATG 9 cut(s) 68, 273, 331, 566, 627, 695, 740, 894, 943
FaqI GGGAC 1 cut(s) 343
FatI CATG 9 cut(s) 64, 269, 327, 562, 623, 691, 736, 890, 939
FauI CCCGC 1 cut(s) 146
FbaI TGATCA 2 cut(s) 70, 319
Fnu4HI GCNGC 1 cut(s) 495
FokI GGATG 1 cut(s) 743
Fsp4HI GCNGC 1 cut(s) 495
FspBI CTAG 3 cut(s) 362, 584, 1059
GlaI GCGC 3 cut(s) 461, 493, 957
GluI GCNGC 1 cut(s) 495
GsuI CTGGAG 2 cut(s) 92, 1066
HaeIII GGCC 2 cut(s) 192, 1079
HapII CCGG 3 cut(s) 243, 265, 885
HgaI GACGC 2 cut(s) 1140, 1157
HhaI GCGC 3 cut(s) 462, 494, 958
Hin1II CATG 9 cut(s) 68, 273, 331, 566, 627, 695, 740, 894, 943
Hin6I GCGC 3 cut(s) 460, 492, 956
HinP1I GCGC 3 cut(s) 460, 492, 956
HincII GTYRAC 1 cut(s) 1129
HindII GTYRAC 1 cut(s) 1129
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 6 cut(s) 293, 409, 414, 602, 674, 1041
HpaII CCGG 3 cut(s) 243, 265, 885
HphI GGTGA 6 cut(s) 80, 158, 169, 208, 386, 497
Hpy166II GTNNAC 2 cut(s) 568, 1129
Hpy188I TCNGA 8 cut(s) 51, 79, 97, 253, 507, 858, 1003, 1147
Hpy188III TCNNGA 6 cut(s) 146, 225, 398, 418, 594, 1045
Hpy8I GTNNAC 2 cut(s) 568, 1129
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 3 cut(s) 129, 528, 1082
HpyCH4III ACNGT 4 cut(s) 574, 1126, 1172, 1187
HpyCH4IV ACGT 2 cut(s) 288, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 508, 707
HpyF3I CTNAG 6 cut(s) 48, 96, 463, 498, 857, 959
HpySE526I ACGT 2 cut(s) 288, 470
Hsp92II CATG 9 cut(s) 68, 273, 331, 566, 627, 695, 740, 894, 943
HspAI GCGC 3 cut(s) 460, 492, 956
Ksp22I TGATCA 2 cut(s) 70, 319
Kzo9I GATC 7 cut(s) 44, 70, 79, 151, 319, 400, 628
LguI GCTCTTC 3 cut(s) 253, 389, 539
LmnI GCTCC 2 cut(s) 347, 648
Lsp1109I GCAGC 1 cut(s) 481
LweI GCATC 2 cut(s) 498, 688
MabI ACCWGGT 1 cut(s) 519
MaeI CTAG 3 cut(s) 362, 584, 1059
MaeII ACGT 2 cut(s) 288, 470
MaeIII GTNAC 7 cut(s) 374, 485, 603, 679, 893, 935, 1187
MalI GATC 7 cut(s) 46, 72, 81, 153, 321, 402, 630
MboI GATC 7 cut(s) 44, 70, 79, 151, 319, 400, 628
MboII GAAGA 6 cut(s) 161, 240, 376, 556, 790, 931
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 3 cut(s) 394, 551, 653
MluCI AATT 7 cut(s) 55, 366, 423, 765, 810, 832, 1156
MluI ACGCGT 1 cut(s) 1149
MlyI GAGTC 4 cut(s) 302, 423, 611, 683
MmeI TCCRAC 2 cut(s) 276, 1065
MnlI CCTC 9 cut(s) 116, 139, 185, 220, 294, 721, 925, 956, 1043
MroXI GAANNNNTTC 1 cut(s) 1066
MseI TTAA 2 cut(s) 768, 1115
MslI CAYNNNNRTG 2 cut(s) 274, 696
MspI CCGG 3 cut(s) 243, 265, 885
MspR9I CCNGG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 521
MvnI CGCG 3 cut(s) 460, 492, 1151
MwoI GCNNNNNNNGC 2 cut(s) 508, 707
NdeII GATC 7 cut(s) 44, 70, 79, 151, 319, 400, 628
NlaIII CATG 9 cut(s) 68, 273, 331, 566, 627, 695, 740, 894, 943
NmuCI GTSAC 6 cut(s) 374, 485, 603, 679, 893, 1187
NspI RCATGY 2 cut(s) 740, 894
PciI ACATGT 1 cut(s) 736
PciSI GCTCTTC 3 cut(s) 253, 389, 539
PdmI GAANNNNTTC 1 cut(s) 1066
PfeI GAWTC 2 cut(s) 409, 1041
PflFI GACNNNGTC 1 cut(s) 572
PflMI CCANNNNNTGG 1 cut(s) 482
PkrI GCNGC 1 cut(s) 496
PleI GAGTC 4 cut(s) 301, 422, 610, 682
PpsI GAGTC 4 cut(s) 301, 422, 610, 682
PscI ACATGT 1 cut(s) 736
Psp6I CCWGG 1 cut(s) 519
PspEI GGTNACC 1 cut(s) 485
PspGI CCWGG 1 cut(s) 519
PspPI GGNCC 3 cut(s) 523, 900, 1100
PsrI GAACNNNNNNTAC 2 cut(s) 8, 40
PstI CTGCAG 2 cut(s) 340, 1027
PsuI RGATCY 1 cut(s) 44
PsyI GACNNNGTC 1 cut(s) 572
RsaI GTAC 3 cut(s) 26, 455, 881
RsaNI GTAC 3 cut(s) 25, 454, 880
RseI CAYNNNNRTG 2 cut(s) 274, 696
SapI GCTCTTC 3 cut(s) 253, 389, 539
SaqAI TTAA 2 cut(s) 768, 1115
SatI GCNGC 1 cut(s) 495
Sau3AI GATC 7 cut(s) 44, 70, 79, 151, 319, 400, 628
Sau96I GGNCC 3 cut(s) 523, 900, 1100
ScaI AGTACT 1 cut(s) 455
SchI GAGTC 4 cut(s) 302, 423, 611, 683
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 3 cut(s) 394, 551, 653
SexAI ACCWGGT 1 cut(s) 519
SfaNI GCATC 2 cut(s) 498, 688
SfcI CTRYAG 3 cut(s) 336, 1023, 1122
SinI GGWCC 3 cut(s) 523, 900, 1100
SmiMI CAYNNNNRTG 2 cut(s) 274, 696
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
Sse9I AATT 7 cut(s) 55, 366, 423, 765, 810, 832, 1156
SsiI CCGC 3 cut(s) 139, 490, 1176
SspMI CTAG 3 cut(s) 362, 584, 1059
StyD4I CCNGG 1 cut(s) 519
TaaI ACNGT 4 cut(s) 574, 1126, 1172, 1187
TaiI ACGT 2 cut(s) 291, 473
TaqI TCGA 4 cut(s) 291, 412, 631, 726
TasI AATT 7 cut(s) 55, 366, 423, 765, 810, 832, 1156
TatI WGTACW 2 cut(s) 453, 879
TfiI GAWTC 2 cut(s) 409, 1041
Tru1I TTAA 2 cut(s) 768, 1115
Tru9I TTAA 2 cut(s) 768, 1115
TscAI CASTG 9 cut(s) 176, 357, 394, 577, 717, 868, 999, 1139, 1177
TseFI GTSAC 6 cut(s) 374, 485, 603, 679, 893, 1187
TseI GCWGC 1 cut(s) 494
Tsp45I GTSAC 6 cut(s) 374, 485, 603, 679, 893, 1187
TspDTI ATGAA 4 cut(s) 53, 223, 450, 612
TspGWI ACGGA 1 cut(s) 12
TspRI CASTG 9 cut(s) 176, 357, 394, 577, 717, 868, 999, 1139, 1177
Tth111I GACNNNGTC 1 cut(s) 572
Van91I CCANNNNNTGG 1 cut(s) 482
VpaK11BI GGWCC 3 cut(s) 523, 900, 1100
XceI RCATGY 2 cut(s) 740, 894
XcmI CCANNNNNNNNNTGG 1 cut(s) 985
XmnI GAANNNNTTC 1 cut(s) 1066
XspI CTAG 3 cut(s) 362, 584, 1059
ZrmI AGTACT 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.