AT5G50860

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014887
Physical Location & Seq
Reverse (-)
20693455 .. 20697233
3779 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50860.2

Sequence Viewer

Length: 1743 bp
ATGGGATGCGTTTTATGCAAAGAATCCACCGGAGACAAACGGAAACACAATAATCCCGATGAACCTCCTCCGGCAGACCTCCGTAATACGGAGGATTTACCTTCTTCTTCTTCAACCACCACCGCCGCCATTTCTGTCGAAATCGGAGAGAAGAAGAAGAAAGATTTAGATTCGATTCAGATTCAGCCGGAGAGAAGAACATGGCACACTGGCGACTTCTCCGCCGGCTCTTCTCGCCGTCCGGGGATGAGTCTCCGTACGCCGGAAGGATGGCCACCTTGGCTTATTGCTGCTTGTGGCGATTCGATCAAAGACTTAACACCTCGCAGAGCTACCACATACGAAAAGCTCGAGAAGATTGGGCAAGGAACGTATAGCAATGTGTACAAGGCGAAAGATTTATTGAGTGGGAAGATTGTAGCTTTGAAGAAAGTTAGGTTTGATAATTTGGAAGCTGAGAGTGTTAAATTTATGGCTAGAGAGATTCTTGTGTTACGACGGCTTAATCATCCTAATGTTATTAAGCTTCAAGGTTTGGTTACTTCACGGGTTTCCTGTAGTCTCTACCTTGTTTTTGAGTATATGGAGCATGATCTTTCTGGTCTTGCTGCTACCCAAGGTCTCAAGTTTGATCTTCCTCAGGTTAAATGCTTTATGAAGCAGCTACTATCTGGATTGGAGCATTGCCATAGTCGAGGAGTATTACATCGGGATATCAAAGGTTCTAATCTTCTTATAGATAATGATGGAATACTGAAGATTGCTGATTTCGGGTTGGCTACGTTTTATGACCCAAAGCAAAAGCAAACAATGACTAGTCGTGTTGTCACACTTTGGTACCGTCCTCCCGAGCTTCTTCTTGGAGCTACCAGTTATGGAACTGGTGTTGATCTTTGGAGTGCTGGTTGTATTATGGCTGAGTTACTTGCTGGCAAGCCCGTTATGCCGGGAAGAACCGAGGTCGAACAACTTCATAAGATATTCAAATTATGTGGCTCCCCTTCAGATTCGTACTGGAAGAAGTACAGACTGCCAAACGCAACTCTTTTTAAGCCGCAACATCCGTACAAACGTTGTGTGGCTGAAGCCTTCAATGGTTTCACTCCATCCTCTGTGCATCTAGTTGAGACACTTCTCACTATAGACCCTGCTGATCGAGGAACTTCTACCTCGGCATTGAATAGTGAATTCTTTACTACTGAACCGTTACCTTGTGATCCTTCAAGTTTACCAAAGTATCCACCTTCCAAAGAACTGAATGTAAAGCTGAGGGACGAAGAGTTAAGAAGGCAAAAGGGTCTTGCTGGAAAAGGTAGTGGTATTGATGGAGCTAGAAGAATAAGGTATCGAGGGGATCGCACTGGGCGAGCTATTCCAGCCCCAGAAGCTAATGCCGAGAGTCAAGCAAACTTAGATAGATGGAGATCGATATCACAAACAAATGGAAAGAGCAAGAGTGAGAAGTTTCCGCCTCCTCACCAGGACGGTGCAGTTGGGTACCCATTGGAAGATCTATCAAAGAAAACCTCAGTATTTGGTGCAAAGACTGAGACTTCTTTTGGGTTATCGAGATCATTAAAGTCTGGGGAAGGGACATCAATGAGAAAGATTTCAAACAAAGACGGGGCTCGTGGGGCTTCTTCTAGAAAGTACATATGGGGACTTAAACCTCCTCCAGCACTCGGGCTATCGATGGACTTACTTTTCAGGAGCAGATCAGAAGTTTTTGGGATCCGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

580

Amino Acids

64.29

Weight (kDa)

9.51

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 114 - 398 6.8e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 116 - 327 1.3e-31 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 180 - 277 5.6e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50860 AT5G50860
fragaria_vesca FvH4_5g09040
malus_domestica MD06G1134300.v1.1 MD14G1150700.v1.1
prunus_persica Prupe.5G145300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189181
rosa_laevigata RLG00000004626
rosa_multiflora Rmu_ssc0000251.1_g000001
rosa_roxburghii Rroxscaffold_3G00265730
rosa_rugosa Rorug06G0496600
rosa_samantha Rh7AG101200 Rh7BG103800 Rh7CG105200 Rh7DG103100
rosa_wichuraiana Rw7G008740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 837, 1497
AccB1I GGYRCC 2 cut(s) 837, 1497
AccIII TCCGGA 1 cut(s) 1734
AciI CCGC 5 cut(s) 123, 126, 222, 1055, 1469
AclI AACGTT 1 cut(s) 1072
AclWI GGATC 4 cut(s) 1211, 1362, 1726, 1739
AcoI YGGCCR 1 cut(s) 272
AcsI RAATTY 2 cut(s) 467, 1187
AcuI CTGAAG 3 cut(s) 776, 987, 1104
AfaI GTAC 8 cut(s) 259, 386, 839, 1013, 1025, 1067, 1499, 1652
AfiI CCNNNNNNNGG 3 cut(s) 88, 262, 1682
AgsI TTSAA 8 cut(s) 114, 427, 530, 985, 1093, 1180, 1224, 1614
AhlI ACTAGT 1 cut(s) 815
AjnI CCWGG 1 cut(s) 1479
Alw26I GTCTC 6 cut(s) 27, 257, 566, 626, 1121, 1544
AlwI GGATC 4 cut(s) 1211, 1362, 1726, 1739
Ama87I CYCGRG 3 cut(s) 350, 848, 1682
Aor13HI TCCGGA 1 cut(s) 1734
AoxI GGCC 1 cut(s) 272
ApeKI GCWGC 3 cut(s) 290, 608, 661
ApoI RAATTY 2 cut(s) 467, 1187
Asp700I GAANNNNTTC 2 cut(s) 969, 1609
Asp718I GGTACC 2 cut(s) 837, 1497
AsuC2I CCSGG 2 cut(s) 243, 948
AsuHPI GGTGA 1 cut(s) 1469
AvaI CYCGRG 3 cut(s) 350, 848, 1682
AxyI CCTNAGG 1 cut(s) 639
BaeI ACNNNNGTAYC 4 cut(s) 821, 854, 1220, 1253
BalI TGGCCA 1 cut(s) 274
BamHI GGATCC 1 cut(s) 1731
BanI GGYRCC 2 cut(s) 837, 1497
BanII GRGCYC 1 cut(s) 1630
BauI CACGAG 1 cut(s) 1629
BbvCI CCTCAGC 1 cut(s) 1268
BbvI GCAGC 3 cut(s) 277, 595, 673
BccI CCATC 6 cut(s) 264, 740, 1114, 1319, 1413, 1687
BceAI ACGGC 2 cut(s) 222, 515
BciT130I CCWGG 1 cut(s) 1481
BciVI GTATCC 1 cut(s) 1248
BcnI CCSGG 2 cut(s) 243, 948
BcoDI GTCTC 6 cut(s) 27, 257, 566, 626, 1121, 1544
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 5 cut(s) 477, 816, 1121, 1332, 1644
BfmI CTRYAG 2 cut(s) 556, 1140
BfuI GTATCC 1 cut(s) 1248
BglI GCCNNNNNGGC 1 cut(s) 280
BglII AGATCT 1 cut(s) 1510
BisI GCNGC 5 cut(s) 126, 291, 609, 662, 1055
BlsI GCNGC 5 cut(s) 127, 292, 610, 663, 1056
Bme1390I CCNGG 3 cut(s) 243, 948, 1481
BmeT110I CYCGRG 3 cut(s) 350, 848, 1682
BmiI GGNNCC 4 cut(s) 839, 997, 1499, 1733
BmrFI CCNGG 3 cut(s) 243, 948, 1481
BmrI ACTGGG 1 cut(s) 1371
BmsI GCATC 1 cut(s) 1126
BmuI ACTGGG 1 cut(s) 1371
BpmI CTGGAG 1 cut(s) 1659
Bpu10I CCTNAGC 1 cut(s) 1268
BpuEI CTTGAG 1 cut(s) 608
BpuMI CCSGG 2 cut(s) 243, 948
Bsa29I ATCGAT 2 cut(s) 1427, 1691
BsaBI GATNNNNATC 2 cut(s) 1423, 1429
BsaI GGTCTC 1 cut(s) 626
BsaJI CCNNGG 5 cut(s) 242, 278, 616, 957, 1170
BsaWI WCCGGW 2 cut(s) 29, 1734
BsaXI ACNNNNNCTCC 2 cut(s) 889, 919
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 262, 1682
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 5 cut(s) 214, 870, 886, 1019, 1366
Bse21I CCTNAGG 1 cut(s) 639
Bse3DI GCAATG 2 cut(s) 385, 682
Bse8I GATNNNNATC 2 cut(s) 1423, 1429
BseAI TCCGGA 1 cut(s) 1734
BseBI CCWGG 1 cut(s) 1481
BseCI ATCGAT 2 cut(s) 1427, 1691
BseDI CCNNGG 5 cut(s) 242, 278, 616, 957, 1170
BseGI GGATG 6 cut(s) 11, 252, 275, 508, 1060, 1106
BseJI GATNNNNATC 2 cut(s) 1423, 1429
BseLI CCNNNNNNNGG 3 cut(s) 88, 262, 1682
BseMI GCAATG 2 cut(s) 385, 682
BseMII CTCAG 6 cut(s) 447, 653, 909, 1259, 1539, 1542
BseNI ACTGG 5 cut(s) 214, 870, 886, 1019, 1366
BseRI GAGGAG 4 cut(s) 57, 711, 1464, 1662
BseXI GCAGC 3 cut(s) 277, 595, 673
BsgI GTGCAG 1 cut(s) 1509
BshFI GGCC 1 cut(s) 274
BshNI GGYRCC 2 cut(s) 837, 1497
BshVI ATCGAT 2 cut(s) 1427, 1691
BsiHKCI CYCGRG 3 cut(s) 350, 848, 1682
BsiSI CCGG 8 cut(s) 30, 71, 188, 225, 242, 263, 947, 1735
BsiWI CGTACG 1 cut(s) 257
BslFI GGGAC 3 cut(s) 1286, 1606, 1674
BslI CCNNNNNNNGG 3 cut(s) 88, 262, 1682
BsmAI GTCTC 6 cut(s) 27, 257, 566, 626, 1121, 1544
BsmFI GGGAC 3 cut(s) 1286, 1606, 1674
BsnI GGCC 1 cut(s) 274
Bso31I GGTCTC 1 cut(s) 626
BsoBI CYCGRG 3 cut(s) 350, 848, 1682
Bsp1286I GDGCHC 1 cut(s) 1630
Bsp13I TCCGGA 1 cut(s) 1734
Bsp1407I TGTACA 1 cut(s) 384
BspACI CCGC 5 cut(s) 123, 126, 222, 1055, 1469
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 6 cut(s) 448, 652, 910, 1260, 1540, 1541
BspDI ATCGAT 2 cut(s) 1427, 1691
BspEI TCCGGA 1 cut(s) 1734
BspLI GGNNCC 4 cut(s) 839, 997, 1499, 1733
BspPI GGATC 4 cut(s) 1211, 1362, 1726, 1739
BspQI GCTCTTC 1 cut(s) 235
BspT107I GGYRCC 2 cut(s) 837, 1497
BspTNI GGTCTC 1 cut(s) 626
BsrDI GCAATG 2 cut(s) 385, 682
BsrFI RCCGGY 1 cut(s) 224
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 5 cut(s) 214, 870, 886, 1019, 1366
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 5 cut(s) 242, 278, 616, 957, 1170
BssSI CACGAG 1 cut(s) 1629
BssT1I CCWWGG 2 cut(s) 278, 616
Bst2BI CACGAG 1 cut(s) 1629
Bst2UI CCWGG 1 cut(s) 1481
Bst4CI ACNGT 3 cut(s) 842, 1206, 1487
Bst6I CTCTTC 2 cut(s) 235, 1272
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 4 cut(s) 226, 931, 935, 1368
BstDEI CTNAG 7 cut(s) 456, 639, 918, 1268, 1411, 1528, 1548
BstF5I GGATG 6 cut(s) 11, 252, 275, 508, 1060, 1106
BstMAI GTCTC 6 cut(s) 27, 257, 566, 626, 1121, 1544
BstMWI GCNNNNNNNGC 7 cut(s) 15, 234, 280, 943, 1376, 1385, 1634
BstNI CCWGG 1 cut(s) 1481
BstSCI CCNGG 3 cut(s) 241, 946, 1479
BstSFI CTRYAG 2 cut(s) 556, 1140
BstV1I GCAGC 3 cut(s) 277, 595, 673
BstX2I RGATCY 2 cut(s) 1510, 1731
BstYI RGATCY 2 cut(s) 1510, 1731
Bsu15I ATCGAT 2 cut(s) 1427, 1691
Bsu36I CCTNAGG 1 cut(s) 639
BsuI GTATCC 1 cut(s) 1248
BsuRI GGCC 1 cut(s) 274
BsuTUI ATCGAT 2 cut(s) 1427, 1691
BtsCI GGATG 6 cut(s) 11, 252, 275, 508, 1060, 1106
BtsIMutI CAGTG 2 cut(s) 207, 1359
Cac8I GCNNGC 4 cut(s) 226, 931, 935, 1368
Cfr10I RCCGGY 1 cut(s) 224
ClaI ATCGAT 2 cut(s) 1427, 1691
Csp6I GTAC 8 cut(s) 258, 385, 838, 1012, 1024, 1066, 1498, 1651
CspCI CAANNNNNGTGG 2 cut(s) 973, 1008
CviAII CATG 2 cut(s) 201, 590
CviQI GTAC 8 cut(s) 258, 385, 838, 1012, 1024, 1066, 1498, 1651
DdeI CTNAG 7 cut(s) 456, 639, 918, 1268, 1411, 1528, 1548
EaeI YGGCCR 1 cut(s) 272
Eam1104I CTCTTC 2 cut(s) 235, 1272
EarI CTCTTC 2 cut(s) 235, 1272
EciI GGCGGA 2 cut(s) 211, 1458
Eco130I CCWWGG 2 cut(s) 278, 616
Eco24I GRGCYC 1 cut(s) 1630
Eco31I GGTCTC 1 cut(s) 626
Eco32I GATATC 2 cut(s) 715, 1431
Eco57I CTGAAG 3 cut(s) 776, 987, 1104
Eco81I CCTNAGG 1 cut(s) 639
Eco88I CYCGRG 3 cut(s) 350, 848, 1682
EcoRI GAATTC 1 cut(s) 1187
EcoRII CCWGG 1 cut(s) 1479
EcoRV GATATC 2 cut(s) 715, 1431
EcoT14I CCWWGG 2 cut(s) 278, 616
EcoT38I GRGCYC 1 cut(s) 1630
ErhI CCWWGG 2 cut(s) 278, 616
FaeI CATG 2 cut(s) 204, 593
FaqI GGGAC 3 cut(s) 1286, 1606, 1674
FatI CATG 2 cut(s) 200, 589
FauNDI CATATG 1 cut(s) 1655
Fnu4HI GCNGC 5 cut(s) 126, 291, 609, 662, 1055
FokI GGATG 6 cut(s) 18, 259, 282, 495, 1047, 1093
FriOI GRGCYC 1 cut(s) 1630
Fsp4HI GCNGC 5 cut(s) 126, 291, 609, 662, 1055
FspBI CTAG 5 cut(s) 477, 816, 1121, 1332, 1644
GluI GCNGC 5 cut(s) 126, 291, 609, 662, 1055
GsuI CTGGAG 1 cut(s) 1659
HaeIII GGCC 1 cut(s) 274
HapII CCGG 8 cut(s) 30, 71, 188, 225, 242, 263, 947, 1735
Hin1II CATG 2 cut(s) 204, 593
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 9 cut(s) 23, 170, 175, 181, 250, 302, 484, 1007, 1399
HpaII CCGG 8 cut(s) 30, 71, 188, 225, 242, 263, 947, 1735
HphI GGTGA 1 cut(s) 1469
Hpy166II GTNNAC 2 cut(s) 385, 1229
Hpy188I TCNGA 4 cut(s) 146, 180, 1006, 1720
Hpy188III TCNNGA 9 cut(s) 56, 352, 672, 710, 848, 1569, 1644, 1708, 1735
Hpy8I GTNNAC 2 cut(s) 385, 1229
Hpy99I CGWCG 1 cut(s) 501
HpyAV CCTTC 8 cut(s) 111, 260, 1011, 1099, 1230, 1254, 1281, 1583
HpyCH4III ACNGT 3 cut(s) 842, 1206, 1487
HpyCH4IV ACGT 3 cut(s) 371, 782, 1072
HpyCH4V TGCA 4 cut(s) 18, 1117, 1490, 1541
HpyF10VI GCNNNNNNNGC 7 cut(s) 15, 234, 280, 943, 1376, 1385, 1634
HpyF3I CTNAG 7 cut(s) 456, 639, 918, 1268, 1411, 1528, 1548
HpySE526I ACGT 3 cut(s) 371, 782, 1072
Hsp92II CATG 2 cut(s) 204, 593
Kpn2I TCCGGA 1 cut(s) 1734
KpnI GGTACC 2 cut(s) 841, 1501
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
LguI GCTCTTC 1 cut(s) 235
LmnI GCTCC 6 cut(s) 586, 679, 863, 1001, 1328, 1710
Lsp1109I GCAGC 3 cut(s) 277, 595, 673
LweI GCATC 1 cut(s) 1126
MaeI CTAG 5 cut(s) 477, 816, 1121, 1332, 1644
MaeII ACGT 3 cut(s) 371, 782, 1072
MaeIII GTNAC 5 cut(s) 492, 538, 826, 921, 1206
MflI RGATCY 2 cut(s) 1510, 1731
MhlI GDGCHC 1 cut(s) 1630
MlsI TGGCCA 1 cut(s) 274
MluCI AATT 4 cut(s) 445, 467, 986, 1187
MluNI TGGCCA 1 cut(s) 274
MlyI GAGTC 2 cut(s) 259, 1408
Mox20I TGGCCA 1 cut(s) 274
MroI TCCGGA 1 cut(s) 1734
MroNI GCCGGC 1 cut(s) 224
MroXI GAANNNNTTC 2 cut(s) 969, 1609
MscI TGGCCA 1 cut(s) 274
MseI TTAA 9 cut(s) 317, 465, 504, 522, 645, 1050, 1283, 1577, 1665
MslI CAYNNNNRTG 1 cut(s) 513
Msp20I TGGCCA 1 cut(s) 274
MspI CCGG 8 cut(s) 30, 71, 188, 225, 242, 263, 947, 1735
MspR9I CCNGG 3 cut(s) 243, 948, 1481
MvaI CCWGG 1 cut(s) 1481
MwoI GCNNNNNNNGC 7 cut(s) 15, 234, 280, 943, 1376, 1385, 1634
NaeI GCCGGC 1 cut(s) 226
NciI CCSGG 2 cut(s) 243, 948
NdeI CATATG 1 cut(s) 1655
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 2 cut(s) 204, 593
NlaIV GGNNCC 4 cut(s) 839, 997, 1499, 1733
NmeAIII GCCGAG 2 cut(s) 1151, 1420
NmuCI GTSAC 1 cut(s) 826
PaeR7I CTCGAG 1 cut(s) 350
PciSI GCTCTTC 1 cut(s) 235
PcsI WCGNNNNNNNCGW 2 cut(s) 348, 1363
PdiI GCCGGC 1 cut(s) 226
PdmI GAANNNNTTC 2 cut(s) 969, 1609
PfeI GAWTC 7 cut(s) 23, 170, 175, 181, 302, 484, 1007
Pfl23II CGTACG 1 cut(s) 257
PkrI GCNGC 5 cut(s) 127, 292, 610, 663, 1056
PleI GAGTC 2 cut(s) 258, 1407
PpsI GAGTC 2 cut(s) 258, 1407
Psp1406I AACGTT 1 cut(s) 1072
Psp6I CCWGG 1 cut(s) 1479
PspGI CCWGG 1 cut(s) 1479
PspLI CGTACG 1 cut(s) 257
PspN4I GGNNCC 4 cut(s) 839, 997, 1499, 1733
PsuI RGATCY 2 cut(s) 1510, 1731
RsaI GTAC 8 cut(s) 259, 386, 839, 1013, 1025, 1067, 1499, 1652
RsaNI GTAC 8 cut(s) 258, 385, 838, 1012, 1024, 1066, 1498, 1651
RseI CAYNNNNRTG 1 cut(s) 513
SapI GCTCTTC 1 cut(s) 235
SaqAI TTAA 9 cut(s) 317, 465, 504, 522, 645, 1050, 1283, 1577, 1665
SatI GCNGC 5 cut(s) 126, 291, 609, 662, 1055
SchI GAGTC 2 cut(s) 259, 1408
ScrFI CCNGG 3 cut(s) 243, 948, 1481
SduI GDGCHC 1 cut(s) 1630
SfaNI GCATC 1 cut(s) 1126
SfcI CTRYAG 2 cut(s) 556, 1140
Sfr274I CTCGAG 1 cut(s) 350
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 513
SmlI CTYRAG 2 cut(s) 350, 623
SmoI CTYRAG 2 cut(s) 350, 623
SpeI ACTAGT 1 cut(s) 815
Sse9I AATT 4 cut(s) 445, 467, 986, 1187
SsiI CCGC 5 cut(s) 123, 126, 222, 1055, 1469
SspMI CTAG 5 cut(s) 477, 816, 1121, 1332, 1644
StyD4I CCNGG 3 cut(s) 241, 946, 1479
StyI CCWWGG 2 cut(s) 278, 616
TaaI ACNGT 3 cut(s) 842, 1206, 1487
TaiI ACGT 3 cut(s) 374, 785, 1075
TasI AATT 4 cut(s) 445, 467, 986, 1187
TatI WGTACW 3 cut(s) 384, 1023, 1650
TauI GCSGC 2 cut(s) 128, 1057
TfiI GAWTC 7 cut(s) 23, 170, 175, 181, 302, 484, 1007
Tru1I TTAA 9 cut(s) 317, 465, 504, 522, 645, 1050, 1283, 1577, 1665
Tru9I TTAA 9 cut(s) 317, 465, 504, 522, 645, 1050, 1283, 1577, 1665
TscAI CASTG 2 cut(s) 214, 1366
TseFI GTSAC 1 cut(s) 826
TseI GCWGC 3 cut(s) 290, 608, 661
Tsp45I GTSAC 1 cut(s) 826
TspDTI ATGAA 3 cut(s) 75, 671, 962
TspGWI ACGGA 5 cut(s) 55, 71, 104, 245, 1053
TspRI CASTG 2 cut(s) 214, 1366
XapI RAATTY 2 cut(s) 467, 1187
XbaI TCTAGA 1 cut(s) 1643
XhoI CTCGAG 1 cut(s) 350
XmnI GAANNNNTTC 2 cut(s) 969, 1609
XspI CTAG 5 cut(s) 477, 816, 1121, 1332, 1644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.