Rroxscaffold_3G00265730

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014887
Physical Location & Seq
Reverse (-)
59131902 .. 59136572
4671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00265730.1

Sequence Viewer

Length: 783 bp
ATGGGGTGCGTTTTCGGCAAGAGTTCCGCCGGCTGTGGCGGAGGAGATGGTAGTCGGCGGCATCGCAGCCGGAGTGTCGAGGAGCCGTCCCACGCCGCCGCCGCCGCCGCCGTCACCGGCGGAGGGGTTGTTAGGGATAAGAAGGAAGCGGTGGAGAGGCAGAGAGTGAATCATCACACCGGCGACTTTCCGGTGGTTGAGCGGCGGAAGCCGTTTCAGGTGAACCAGCAAGGCTGGCCGTCGTGGCTATTGGCCGTCGCCGGCGACGCGATCCAGGGATGGACGCCGCGGCGGGCCAACACTTTCGAGAAGCTTTCAAAGATTGGGCAAGGGACTTATAGCAATGTATATAAAGCCAGAGACTTAATTTCTGGGAAAATTGTGGCGTTAAAGAAGGTTAGATTTGATAACTTGGAGCCGGAGAGTGTGAAATTCATGGCCAGAGAGATTAATGTGCTGCGGAAACTTGACCATCCCAATGTGGTTAAGCTTGAAGGTCTAGTCACTTCAAGAATGTCATGTAGTCTCTACTTGGTTTTCGAGTACATGGAGCATGATCTCTCCGGCCTAGCCGCCTCCACGGGGATCAAGTTCACCGAGTCTCAGGTCAAGTGCTATATGAAACAGTTGCTGTCTGGCCTTGAACATTGCCACAACCACGGAGTCTTGCACCGTGATATCAAGGGGTCTAATCTACTCATTGACAATGAAGGAGTTCTGAAAATAGCTGATTTTGGATTGGCTACATTTTTTTTATCCTCATCAGAAGCAACCCTTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

260

Amino Acids

28.6

Weight (kDa)

9.17

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 102 - 256 5e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 105 - 251 9.9e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50860 AT5G50860
fragaria_vesca FvH4_5g09040
malus_domestica MD06G1134300.v1.1 MD14G1150700.v1.1
prunus_persica Prupe.5G145300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189181
rosa_laevigata RLG00000004626
rosa_multiflora Rmu_ssc0000251.1_g000001
rosa_roxburghii Rroxscaffold_3G00265730
rosa_rugosa Rorug06G0496600
rosa_samantha Rh7AG101200 Rh7BG103800 Rh7CG105200 Rh7DG103100
rosa_wichuraiana Rw7G008740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 202
AccII CGCG 2 cut(s) 269, 289
AclWI GGATC 2 cut(s) 265, 593
AcoI YGGCCR 3 cut(s) 236, 252, 438
AcsI RAATTY 1 cut(s) 431
AcyI GRCGYC 1 cut(s) 284
AfaI GTAC 1 cut(s) 545
AfiI CCNNNNNNNGG 2 cut(s) 123, 582
AgsI TTSAA 4 cut(s) 318, 494, 510, 644
AjnI CCWGG 1 cut(s) 273
AluBI AGCT 3 cut(s) 313, 490, 728
AluI AGCT 3 cut(s) 313, 490, 728
Alw26I GTCTC 3 cut(s) 354, 530, 606
AlwI GGATC 2 cut(s) 265, 593
AlwNI CAGNNNCTG 1 cut(s) 631
AoxI GGCC 6 cut(s) 236, 252, 294, 438, 565, 637
ApeKI GCWGC 2 cut(s) 66, 457
ApoI RAATTY 1 cut(s) 431
AseI ATTAAT 1 cut(s) 450
Asp700I GAANNNNTTC 1 cut(s) 714
AspS9I GGNCC 1 cut(s) 294
AsuHPI GGTGA 3 cut(s) 106, 232, 586
BalI TGGCCA 1 cut(s) 440
BbvI GCAGC 2 cut(s) 78, 444
BccI CCATC 3 cut(s) 41, 273, 480
BceAI ACGGC 5 cut(s) 70, 95, 196, 223, 239
BciT130I CCWGG 1 cut(s) 275
BcoDI GTCTC 3 cut(s) 354, 530, 606
BfaI CTAG 3 cut(s) 500, 569, 781
BglI GCCNNNNNGGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 294
BmiI GGNNCC 2 cut(s) 84, 417
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 284
BsaJI CCNNGG 4 cut(s) 274, 287, 579, 658
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 582
Bse118I RCCGGY 4 cut(s) 29, 116, 179, 260
Bse3DI GCAATG 2 cut(s) 349, 646
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 4 cut(s) 274, 287, 579, 658
BseGI GGATG 2 cut(s) 284, 472
BseLI CCNNNNNNNGG 2 cut(s) 123, 582
BseMI GCAATG 2 cut(s) 349, 646
BseMII CTCAG 1 cut(s) 617
BseRI GAGGAG 2 cut(s) 57, 95
BseXI GCAGC 2 cut(s) 78, 444
Bsh1236I CGCG 2 cut(s) 269, 289
BshFI GGCC 6 cut(s) 238, 254, 296, 440, 567, 639
BsiSI CCGG 8 cut(s) 30, 70, 117, 180, 191, 261, 419, 564
BslFI GGGAC 2 cut(s) 73, 346
BslI CCNNNNNNNGG 2 cut(s) 123, 582
BsmAI GTCTC 3 cut(s) 354, 530, 606
BsmFI GGGAC 2 cut(s) 73, 346
BsnI GGCC 6 cut(s) 238, 254, 296, 440, 567, 639
Bsp143I GATC 3 cut(s) 270, 556, 585
BspANI GGCC 6 cut(s) 238, 254, 296, 440, 567, 639
BspCNI CTCAG 1 cut(s) 616
BspFNI CGCG 2 cut(s) 269, 289
BspLI GGNNCC 2 cut(s) 84, 417
BspPI GGATC 2 cut(s) 265, 593
BsrBI CCGCTC 1 cut(s) 202
BsrDI GCAATG 2 cut(s) 349, 646
BsrFI RCCGGY 4 cut(s) 29, 116, 179, 260
BssAI RCCGGY 4 cut(s) 29, 116, 179, 260
BssECI CCNNGG 4 cut(s) 274, 287, 579, 658
BssMI GATC 3 cut(s) 270, 556, 585
BssNI GRCGYC 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 2 cut(s) 627, 674
BstACI GRCGYC 1 cut(s) 284
BstC8I GCNNGC 4 cut(s) 31, 236, 262, 294
BstDEI CTNAG 1 cut(s) 603
BstDSI CCRYGG 3 cut(s) 287, 579, 658
BstF5I GGATG 2 cut(s) 284, 472
BstFNI CGCG 2 cut(s) 269, 289
BstKTI GATC 3 cut(s) 273, 559, 588
BstMAI GTCTC 3 cut(s) 354, 530, 606
BstMBI GATC 3 cut(s) 270, 556, 585
BstMWI GCNNNNNNNGC 8 cut(s) 15, 101, 104, 107, 208, 235, 244, 266
BstNI CCWGG 1 cut(s) 275
BstSCI CCNGG 1 cut(s) 273
BstUI CGCG 2 cut(s) 269, 289
BstV1I GCAGC 2 cut(s) 78, 444
BsuRI GGCC 6 cut(s) 238, 254, 296, 440, 567, 639
BtgI CCRYGG 3 cut(s) 287, 579, 658
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 2 cut(s) 284, 472
Cac8I GCNNGC 4 cut(s) 31, 236, 262, 294
CaiI CAGNNNCTG 1 cut(s) 631
Cfr10I RCCGGY 4 cut(s) 29, 116, 179, 260
Cfr13I GGNCC 1 cut(s) 294
Cfr42I CCGCGG 1 cut(s) 290
CseI GACGC 2 cut(s) 275, 292
Csp6I GTAC 1 cut(s) 544
CviAII CATG 4 cut(s) 436, 519, 547, 554
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 1 cut(s) 603
DpnI GATC 3 cut(s) 272, 558, 587
DpnII GATC 3 cut(s) 270, 556, 585
EaeI YGGCCR 3 cut(s) 236, 252, 438
EciI GGCGGA 4 cut(s) 16, 54, 135, 220
Eco32I GATATC 1 cut(s) 679
EcoRII CCWGG 1 cut(s) 273
EcoRV GATATC 1 cut(s) 679
FaeI CATG 4 cut(s) 439, 522, 550, 557
FaiI YATR 9 cut(s) 339, 349, 351, 437, 520, 548, 555, 618, 620
FalI AAGNNNNNCTT 1 cut(s) 759
FaqI GGGAC 2 cut(s) 73, 346
FatI CATG 4 cut(s) 435, 518, 546, 553
FauI CCCGC 1 cut(s) 285
FokI GGATG 2 cut(s) 291, 459
FspBI CTAG 3 cut(s) 500, 569, 781
HaeIII GGCC 6 cut(s) 238, 254, 296, 440, 567, 639
HapII CCGG 8 cut(s) 30, 70, 117, 180, 191, 261, 419, 564
HgaI GACGC 2 cut(s) 275, 292
Hin1I GRCGYC 1 cut(s) 284
Hin1II CATG 4 cut(s) 439, 522, 550, 557
HindIII AAGCTT 2 cut(s) 311, 488
HinfI GANTC 3 cut(s) 169, 599, 663
HpaII CCGG 8 cut(s) 30, 70, 117, 180, 191, 261, 419, 564
HphI GGTGA 3 cut(s) 106, 232, 586
Hpy166II GTNNAC 2 cut(s) 223, 594
Hpy188I TCNGA 2 cut(s) 720, 766
Hpy188III TCNNGA 2 cut(s) 307, 510
Hpy8I GTNNAC 2 cut(s) 223, 594
Hpy99I CGWCG 3 cut(s) 244, 260, 269
HpyAV CCTTC 4 cut(s) 136, 388, 488, 704
HpyCH4III ACNGT 2 cut(s) 627, 674
HpyCH4V TGCA 1 cut(s) 670
HpyF10VI GCNNNNNNNGC 8 cut(s) 15, 101, 104, 107, 208, 235, 244, 266
HpyF3I CTNAG 1 cut(s) 603
Hsp92I GRCGYC 1 cut(s) 284
Hsp92II CATG 4 cut(s) 439, 522, 550, 557
KroI GCCGGC 2 cut(s) 29, 260
KroNI GCCGGC 2 cut(s) 31, 262
KspI CCGCGG 1 cut(s) 290
Kzo9I GATC 3 cut(s) 270, 556, 585
LmnI GCTCC 3 cut(s) 82, 415, 550
Lsp1109I GCAGC 2 cut(s) 78, 444
LweI GCATC 1 cut(s) 70
MaeI CTAG 3 cut(s) 500, 569, 781
MaeIII GTNAC 2 cut(s) 112, 502
MalI GATC 3 cut(s) 272, 558, 587
MbiI CCGCTC 1 cut(s) 202
MboI GATC 3 cut(s) 270, 556, 585
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 3 cut(s) 366, 378, 431
MluNI TGGCCA 1 cut(s) 440
MlyI GAGTC 2 cut(s) 608, 672
MnlI CCTC 6 cut(s) 35, 73, 116, 150, 586, 769
Mox20I TGGCCA 1 cut(s) 440
MreI CGCCGGCG 1 cut(s) 260
MroNI GCCGGC 2 cut(s) 29, 260
MroXI GAANNNNTTC 1 cut(s) 714
MscI TGGCCA 1 cut(s) 440
MseI TTAA 4 cut(s) 365, 389, 450, 486
MslI CAYNNNNRTG 1 cut(s) 477
Msp20I TGGCCA 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 8 cut(s) 30, 70, 117, 180, 191, 261, 419, 564
MspR9I CCNGG 1 cut(s) 275
MvaI CCWGG 1 cut(s) 275
MvnI CGCG 2 cut(s) 269, 289
MwoI GCNNNNNNNGC 8 cut(s) 15, 101, 104, 107, 208, 235, 244, 266
NaeI GCCGGC 2 cut(s) 31, 262
NdeII GATC 3 cut(s) 270, 556, 585
NgoMIV GCCGGC 2 cut(s) 29, 260
NlaIII CATG 4 cut(s) 439, 522, 550, 557
NlaIV GGNNCC 2 cut(s) 84, 417
NmuCI GTSAC 2 cut(s) 112, 502
PdiI GCCGGC 2 cut(s) 31, 262
PdmI GAANNNNTTC 1 cut(s) 714
PfeI GAWTC 1 cut(s) 169
PleI GAGTC 2 cut(s) 607, 671
PpsI GAGTC 2 cut(s) 607, 671
PshBI ATTAAT 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 2 cut(s) 84, 417
PspPI GGNCC 1 cut(s) 294
PstNI CAGNNNCTG 1 cut(s) 631
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
RseI CAYNNNNRTG 1 cut(s) 477
SacII CCGCGG 1 cut(s) 290
SaqAI TTAA 4 cut(s) 365, 389, 450, 486
Sau3AI GATC 3 cut(s) 270, 556, 585
Sau96I GGNCC 1 cut(s) 294
SchI GAGTC 2 cut(s) 608, 672
ScrFI CCNGG 1 cut(s) 275
SetI ASST 7 cut(s) 222, 315, 399, 492, 499, 609, 730
SfaNI GCATC 1 cut(s) 70
Sfr303I CCGCGG 1 cut(s) 290
SgrAI CRCCGGYG 3 cut(s) 116, 179, 260
SgrBI CCGCGG 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 477
Sse9I AATT 3 cut(s) 366, 378, 431
SspMI CTAG 3 cut(s) 500, 569, 781
StyD4I CCNGG 1 cut(s) 273
TaaI ACNGT 2 cut(s) 627, 674
TaqI TCGA 3 cut(s) 78, 306, 540
TasI AATT 3 cut(s) 366, 378, 431
TatI WGTACW 1 cut(s) 543
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 4 cut(s) 365, 389, 450, 486
Tru9I TTAA 4 cut(s) 365, 389, 450, 486
TseFI GTSAC 2 cut(s) 112, 502
TseI GCWGC 2 cut(s) 66, 457
Tsp45I GTSAC 2 cut(s) 112, 502
TspDTI ATGAA 3 cut(s) 424, 635, 723
TspGWI ACGGA 1 cut(s) 675
VspI ATTAAT 1 cut(s) 450
XapI RAATTY 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 714
XspI CTAG 3 cut(s) 500, 569, 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.