RLG00000004626

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014887
Physical Location & Seq
Reverse (-)
60932426 .. 60936087
3662 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004626

Sequence Viewer

Length: 1383 bp
ATGGGGTGCGTTTTCGGCAAGAGCTCCGCCGGCTGCGGAGGAGGAGGTCGTAGTCGGCGGCATCGCAGCCGGAGCGTCGATGAGCCGTCCGCCTCGGCCGCCGCCGTCACCGGCGGAGGGGTTGTTAGGGATAGGAAGGAAGCGGTGGAGAGGCAGAGAGTGAATCATCACACCGGCGACTTTCCGGTGGTTGAGCGGCGGAAGCCGTTTCAGGTGAACCAGCAAGGCTGGCCGTCGTGGCTATTGGCCGTCGCCGGCGACGCGATCCAGGGATGGACGCCGCGGCGGGCCAACACTTTCGAGAAGCTTTCAAAGATTGGGCAAGGGACTTATAGCAATGTATATAAAGCTAGAGACTTAATTTCTGGGAAAATTGTGGCGTTAAAGAAGGTTAGATTTGATAACTTGGAGCCGGAGAGTGTGAAATTCATGGCCAGAGAGATTAATGTGCTGCGGAAACTTGACCATCCCAATGTGGTTAAGCTTGAAGGTCTAGTCACTTCAAGAATGTCATGTAGTCTCTACTTGGTTTTCGAGTACATGGAGCATGATCTCTCCGGCCTAGTCGCCTCCACGGCGATCAAGTTCACCGAGTCTCAGGTCAAGTGCTATATGAAACAGTTGCTGTCTGGCCTTGAACATTGCCACAACCATGGAGTCTTGCACCGTGATATCAAGGGGTCTAATCTACTAATTGACGATGAAGGAGTTCTGAAAATAGCTGATTTTGGATTGGCTACATTTTTTGATCCTCAACAGAAGCAACCCTTGACTAGTCGGGTTGTTACTCTCTGGTACCGCCCCCCTGAGCTTCTTCTTGGGGCCACTTTTTACGGAGTTGGTGTTGACCTTTGGAGTGCTGGCTGCATTTTGGCAGAGTTGCTTTCCGGGAAGCCTATTATGCCAGGACGGACAGAGGTTGAACAACTGCATAAGATATTCAAGTTATGTGGGTCCCCATCTGAGGATTACTGGAAAAAATACAAATTGCCAAATGCAACACTTTTTAAACCACAGCAGCCATACAAACGCTGCTTGGCAGAAACGTTCAAGGACTTCCCACCTTCTTCCCTACCTTTAATTGAGTCTCTTCTCTCAATAGACTCTGATGGACGAGGCACTGCCACTGCTGCTTTAAATAGTGAATTCTTTACTACTGAGCCTTTTGCTTGTGAGCCATCAAGTTTACCAAAATATCCTCCCAGCAAGGAAATGGATGTAAAGTTACGAGATGCAGAATCTAGAAGGCAAAGAGGTCTAAGTGGGAAGACCAATGCTGATGGTCCAAAAAAAGTCAAAGCTCGTGACCGAGCTGGTCGAGCAATTCCAGTTCCAGAAGCTAATGCAGAGAATCAAGGAAACTTAGACGTATGTGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

461

Amino Acids

50.96

Weight (kDa)

9.18

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 100 - 384 1.7e-66 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 103 - 313 4.8e-32 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 166 - 263 5.8e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50860 AT5G50860
fragaria_vesca FvH4_5g09040
malus_domestica MD06G1134300.v1.1 MD14G1150700.v1.1
prunus_persica Prupe.5G145300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189181
rosa_laevigata RLG00000004626
rosa_multiflora Rmu_ssc0000251.1_g000001
rosa_roxburghii Rroxscaffold_3G00265730
rosa_rugosa Rorug06G0496600
rosa_samantha Rh7AG101200 Rh7BG103800 Rh7CG105200 Rh7DG103100
rosa_wichuraiana Rw7G008740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 795
AccB1I GGYRCC 1 cut(s) 795
AccBSI CCGCTC 1 cut(s) 196
AccII CGCG 2 cut(s) 263, 283
AclI AACGTT 1 cut(s) 1046
AclWI GGATC 2 cut(s) 259, 743
AcoI YGGCCR 4 cut(s) 96, 230, 246, 432
AcsI RAATTY 2 cut(s) 425, 1145
AcyI GRCGYC 1 cut(s) 278
AfaI GTAC 2 cut(s) 539, 797
AfiI CCNNNNNNNGG 2 cut(s) 117, 964
AgsI TTSAA 7 cut(s) 312, 488, 504, 638, 923, 943, 1051
AhlI ACTAGT 1 cut(s) 773
AjnI CCWGG 2 cut(s) 267, 904
AluBI AGCT 9 cut(s) 24, 307, 350, 484, 722, 811, 1301, 1313, 1340
AluI AGCT 9 cut(s) 24, 307, 350, 484, 722, 811, 1301, 1313, 1340
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 4 cut(s) 348, 524, 600, 1092
AlwI GGATC 2 cut(s) 259, 743
AlwNI CAGNNNCTG 1 cut(s) 625
AoxI GGCC 8 cut(s) 96, 230, 246, 288, 432, 559, 631, 822
ApeKI GCWGC 7 cut(s) 33, 66, 451, 864, 1018, 1032, 1130
ApoI RAATTY 2 cut(s) 425, 1145
AseI ATTAAT 1 cut(s) 444
Asp700I GAANNNNTTC 1 cut(s) 708
Asp718I GGTACC 1 cut(s) 795
AspS9I GGNCC 4 cut(s) 288, 822, 954, 1283
AsuC2I CCSGG 1 cut(s) 889
AsuHPI GGTGA 3 cut(s) 100, 226, 580
AvaII GGWCC 2 cut(s) 954, 1283
BalI TGGCCA 1 cut(s) 434
BanI GGYRCC 1 cut(s) 795
BanII GRGCYC 1 cut(s) 26
BauI CACGAG 1 cut(s) 1302
BbsI GAAGAC 1 cut(s) 1274
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 7 cut(s) 20, 78, 438, 851, 1019, 1030, 1117
BccI CCATC 6 cut(s) 267, 474, 967, 1103, 1186, 1274
BceAI ACGGC 6 cut(s) 70, 89, 190, 217, 233, 591
BciT130I CCWGG 2 cut(s) 269, 906
BcnI CCSGG 1 cut(s) 889
BcoDI GTCTC 4 cut(s) 348, 524, 600, 1092
BcuI ACTAGT 1 cut(s) 773
BfaI CTAG 5 cut(s) 351, 494, 563, 774, 1242
BglI GCCNNNNNGGC 2 cut(s) 238, 575
Bme1390I CCNGG 3 cut(s) 269, 889, 906
Bme18I GGWCC 2 cut(s) 954, 1283
BmgT120I GGNCC 4 cut(s) 288, 822, 954, 1283
BmiI GGNNCC 5 cut(s) 411, 797, 823, 955, 956
BmrFI CCNGG 3 cut(s) 269, 889, 906
BmsI GCATC 2 cut(s) 70, 1222
BpiI GAAGAC 1 cut(s) 1274
Bpu10I CCTNAGC 1 cut(s) 807
BpuMI CCSGG 1 cut(s) 889
BsaHI GRCGYC 1 cut(s) 278
BsaJI CCNNGG 5 cut(s) 93, 268, 281, 573, 652
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 828, 858
Bsc4I CCNNNNNNNGG 2 cut(s) 117, 964
Bse118I RCCGGY 4 cut(s) 29, 110, 173, 254
Bse1I ACTGG 2 cut(s) 977, 1328
Bse3DI GCAATG 2 cut(s) 343, 640
BseBI CCWGG 2 cut(s) 269, 906
BseDI CCNNGG 5 cut(s) 93, 268, 281, 573, 652
BseGI GGATG 3 cut(s) 278, 466, 1222
BseLI CCNNNNNNNGG 2 cut(s) 117, 964
BseMI GCAATG 2 cut(s) 343, 640
BseMII CTCAG 4 cut(s) 611, 798, 954, 1149
BseNI ACTGG 2 cut(s) 977, 1328
BseRI GAGGAG 2 cut(s) 54, 57
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 7 cut(s) 20, 78, 438, 851, 1019, 1030, 1117
BseYI CCCAGC 1 cut(s) 1202
Bsh1236I CGCG 2 cut(s) 263, 283
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 8 cut(s) 98, 232, 248, 290, 434, 561, 633, 824
BshNI GGYRCC 1 cut(s) 795
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 26
BsiSI CCGG 9 cut(s) 30, 70, 111, 174, 185, 255, 413, 558, 888
BslFI GGGAC 2 cut(s) 340, 940
BslI CCNNNNNNNGG 2 cut(s) 117, 964
BsmAI GTCTC 4 cut(s) 348, 524, 600, 1092
BsmFI GGGAC 2 cut(s) 340, 940
BsnI GGCC 8 cut(s) 98, 232, 248, 290, 434, 561, 633, 824
Bsp1286I GDGCHC 1 cut(s) 26
Bsp143I GATC 4 cut(s) 264, 550, 579, 748
Bsp19I CCATGG 1 cut(s) 652
BspANI GGCC 8 cut(s) 98, 232, 248, 290, 434, 561, 633, 824
BspCNI CTCAG 4 cut(s) 610, 799, 955, 1150
BspFNI CGCG 2 cut(s) 263, 283
BspLI GGNNCC 5 cut(s) 411, 797, 823, 955, 956
BspPI GGATC 2 cut(s) 259, 743
BspT107I GGYRCC 1 cut(s) 795
BsrBI CCGCTC 1 cut(s) 196
BsrDI GCAATG 2 cut(s) 343, 640
BsrFI RCCGGY 4 cut(s) 29, 110, 173, 254
BsrI ACTGG 2 cut(s) 977, 1328
BssAI RCCGGY 4 cut(s) 29, 110, 173, 254
BssECI CCNNGG 5 cut(s) 93, 268, 281, 573, 652
BssMI GATC 4 cut(s) 264, 550, 579, 748
BssNI GRCGYC 1 cut(s) 278
BssSI CACGAG 1 cut(s) 1302
BssT1I CCWWGG 1 cut(s) 652
Bst2BI CACGAG 1 cut(s) 1302
Bst2UI CCWGG 2 cut(s) 269, 906
Bst4CI ACNGT 2 cut(s) 621, 668
Bst6I CTCTTC 1 cut(s) 1095
BstACI GRCGYC 1 cut(s) 278
BstC8I GCNNGC 5 cut(s) 31, 230, 256, 288, 862
BstDEI CTNAG 6 cut(s) 597, 807, 963, 1158, 1259, 1363
BstDSI CCRYGG 3 cut(s) 281, 573, 652
BstF5I GGATG 3 cut(s) 278, 466, 1222
BstFNI CGCG 2 cut(s) 263, 283
BstKTI GATC 4 cut(s) 267, 553, 582, 751
BstMAI GTCTC 4 cut(s) 348, 524, 600, 1092
BstMBI GATC 4 cut(s) 264, 550, 579, 748
BstMCI CGRYCG 1 cut(s) 99
BstNI CCWGG 2 cut(s) 269, 906
BstSCI CCNGG 3 cut(s) 267, 887, 904
BstUI CGCG 2 cut(s) 263, 283
BstV1I GCAGC 7 cut(s) 20, 78, 438, 851, 1019, 1030, 1117
BstV2I GAAGAC 1 cut(s) 1274
BstXI CCANNNNNNTGG 1 cut(s) 653
BstZI CGGCCG 1 cut(s) 96
BsuRI GGCC 8 cut(s) 98, 232, 248, 290, 434, 561, 633, 824
BtgI CCRYGG 3 cut(s) 281, 573, 652
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 3 cut(s) 278, 466, 1222
BtsI GCAGTG 2 cut(s) 1119, 1125
BtsIMutI CAGTG 2 cut(s) 1119, 1125
Cac8I GCNNGC 5 cut(s) 31, 230, 256, 288, 862
CaiI CAGNNNCTG 1 cut(s) 625
Cfr10I RCCGGY 4 cut(s) 29, 110, 173, 254
Cfr13I GGNCC 4 cut(s) 288, 822, 954, 1283
Cfr42I CCGCGG 1 cut(s) 284
CseI GACGC 3 cut(s) 64, 269, 286
Csp6I GTAC 2 cut(s) 538, 796
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
CviAII CATG 5 cut(s) 430, 513, 541, 548, 653
CviQI GTAC 2 cut(s) 538, 796
DdeI CTNAG 6 cut(s) 597, 807, 963, 1158, 1259, 1363
DpnI GATC 4 cut(s) 266, 552, 581, 750
DpnII GATC 4 cut(s) 264, 550, 579, 748
DraI TTTAAA 2 cut(s) 1009, 1137
EaeI YGGCCR 4 cut(s) 96, 230, 246, 432
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 1095
EarI CTCTTC 1 cut(s) 1095
EciI GGCGGA 4 cut(s) 16, 79, 129, 214
Ecl136II GAGCTC 1 cut(s) 24
EclXI CGGCCG 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 652
Eco24I GRGCYC 1 cut(s) 26
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 954, 1283
Eco52I CGGCCG 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 24
EcoICRI GAGCTC 1 cut(s) 24
EcoO109I RGGNCCY 1 cut(s) 954
EcoRI GAATTC 1 cut(s) 1145
EcoRII CCWGG 2 cut(s) 267, 904
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 652
FaeI CATG 5 cut(s) 433, 516, 544, 551, 656
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FaqI GGGAC 2 cut(s) 340, 940
FatI CATG 5 cut(s) 429, 512, 540, 547, 652
FauI CCCGC 1 cut(s) 279
FokI GGATG 3 cut(s) 285, 453, 1229
FriOI GRGCYC 1 cut(s) 26
FspBI CTAG 5 cut(s) 351, 494, 563, 774, 1242
GsaI CCCAGC 1 cut(s) 1206
HaeIII GGCC 8 cut(s) 98, 232, 248, 290, 434, 561, 633, 824
HapII CCGG 9 cut(s) 30, 70, 111, 174, 185, 255, 413, 558, 888
HgaI GACGC 3 cut(s) 64, 269, 286
Hin1I GRCGYC 1 cut(s) 278
Hin1II CATG 5 cut(s) 433, 516, 544, 551, 656
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HindIII AAGCTT 2 cut(s) 305, 482
HinfI GANTC 7 cut(s) 163, 593, 657, 1085, 1103, 1238, 1351
HpaII CCGG 9 cut(s) 30, 70, 111, 174, 185, 255, 413, 558, 888
HphI GGTGA 3 cut(s) 100, 226, 580
Hpy166II GTNNAC 4 cut(s) 217, 588, 847, 1187
Hpy188I TCNGA 3 cut(s) 714, 964, 1108
Hpy188III TCNNGA 5 cut(s) 301, 504, 1242, 1304, 1334
Hpy8I GTNNAC 4 cut(s) 217, 588, 847, 1187
Hpy99I CGWCG 4 cut(s) 80, 238, 254, 263
HpyAV CCTTC 6 cut(s) 130, 382, 482, 698, 1074, 1239
HpyCH4III ACNGT 2 cut(s) 621, 668
HpyCH4IV ACGT 2 cut(s) 1046, 1368
HpyCH4V TGCA 6 cut(s) 664, 867, 931, 998, 1235, 1346
HpyF3I CTNAG 6 cut(s) 597, 807, 963, 1158, 1259, 1363
HpySE526I ACGT 2 cut(s) 1046, 1368
Hsp92I GRCGYC 1 cut(s) 278
Hsp92II CATG 5 cut(s) 433, 516, 544, 551, 656
KflI GGGWCCC 1 cut(s) 954
KpnI GGTACC 1 cut(s) 799
KroI GCCGGC 2 cut(s) 29, 254
KroNI GCCGGC 2 cut(s) 31, 256
KspI CCGCGG 1 cut(s) 284
Kzo9I GATC 4 cut(s) 264, 550, 579, 748
LmnI GCTCC 4 cut(s) 29, 72, 409, 544
Lsp1109I GCAGC 7 cut(s) 20, 78, 438, 851, 1019, 1030, 1117
LweI GCATC 2 cut(s) 70, 1222
MaeI CTAG 5 cut(s) 351, 494, 563, 774, 1242
MaeII ACGT 2 cut(s) 1046, 1368
MaeIII GTNAC 5 cut(s) 106, 496, 784, 1224, 1304
MalI GATC 4 cut(s) 266, 552, 581, 750
MbiI CCGCTC 1 cut(s) 196
MboI GATC 4 cut(s) 264, 550, 579, 748
MboII GAAGA 4 cut(s) 806, 1059, 1082, 1279
MhlI GDGCHC 1 cut(s) 26
MlsI TGGCCA 1 cut(s) 434
MluCI AATT 8 cut(s) 360, 372, 425, 693, 986, 1080, 1145, 1323
MluNI TGGCCA 1 cut(s) 434
MlyI GAGTC 4 cut(s) 602, 666, 1094, 1097
Mox20I TGGCCA 1 cut(s) 434
MreI CGCCGGCG 1 cut(s) 254
MroNI GCCGGC 2 cut(s) 29, 254
MroXI GAANNNNTTC 1 cut(s) 708
MscI TGGCCA 1 cut(s) 434
MseI TTAA 7 cut(s) 359, 383, 444, 480, 1008, 1079, 1136
MslI CAYNNNNRTG 2 cut(s) 471, 651
Msp20I TGGCCA 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 283
MspI CCGG 9 cut(s) 30, 70, 111, 174, 185, 255, 413, 558, 888
MspR9I CCNGG 3 cut(s) 269, 889, 906
MvaI CCWGG 2 cut(s) 269, 906
MvnI CGCG 2 cut(s) 263, 283
NaeI GCCGGC 2 cut(s) 31, 256
NciI CCSGG 1 cut(s) 889
NcoI CCATGG 1 cut(s) 652
NdeII GATC 4 cut(s) 264, 550, 579, 748
NgoMIV GCCGGC 2 cut(s) 29, 254
NlaIII CATG 5 cut(s) 433, 516, 544, 551, 656
NlaIV GGNNCC 5 cut(s) 411, 797, 823, 955, 956
NmeAIII GCCGAG 1 cut(s) 74
NmuCI GTSAC 3 cut(s) 106, 496, 1304
PdiI GCCGGC 2 cut(s) 31, 256
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 3 cut(s) 163, 1238, 1351
PfoI TCCNGGA 1 cut(s) 887
PleI GAGTC 4 cut(s) 601, 665, 1093, 1097
PpsI GAGTC 4 cut(s) 601, 665, 1093, 1097
PpuMI RGGWCCY 1 cut(s) 954
PshBI ATTAAT 1 cut(s) 444
Psp124BI GAGCTC 1 cut(s) 26
Psp1406I AACGTT 1 cut(s) 1046
Psp5II RGGWCCY 1 cut(s) 954
Psp6I CCWGG 2 cut(s) 267, 904
PspFI CCCAGC 1 cut(s) 1202
PspGI CCWGG 2 cut(s) 267, 904
PspN4I GGNNCC 5 cut(s) 411, 797, 823, 955, 956
PspPI GGNCC 4 cut(s) 288, 822, 954, 1283
PspPPI RGGWCCY 1 cut(s) 954
PstNI CAGNNNCTG 1 cut(s) 625
RsaI GTAC 2 cut(s) 539, 797
RsaNI GTAC 2 cut(s) 538, 796
RseI CAYNNNNRTG 2 cut(s) 471, 651
SacI GAGCTC 1 cut(s) 26
SacII CCGCGG 1 cut(s) 284
SaqAI TTAA 7 cut(s) 359, 383, 444, 480, 1008, 1079, 1136
Sau3AI GATC 4 cut(s) 264, 550, 579, 748
Sau96I GGNCC 4 cut(s) 288, 822, 954, 1283
SchI GAGTC 4 cut(s) 602, 666, 1094, 1097
ScrFI CCNGG 3 cut(s) 269, 889, 906
SduI GDGCHC 1 cut(s) 26
SfaNI GCATC 2 cut(s) 70, 1222
Sfr303I CCGCGG 1 cut(s) 284
SgrAI CRCCGGYG 3 cut(s) 110, 173, 254
SgrBI CCGCGG 1 cut(s) 284
SinI GGWCC 2 cut(s) 954, 1283
SmiMI CAYNNNNRTG 2 cut(s) 471, 651
SpeI ACTAGT 1 cut(s) 773
Sse9I AATT 8 cut(s) 360, 372, 425, 693, 986, 1080, 1145, 1323
SspMI CTAG 5 cut(s) 351, 494, 563, 774, 1242
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 3 cut(s) 267, 887, 904
StyI CCWWGG 1 cut(s) 652
TaaI ACNGT 2 cut(s) 621, 668
TaiI ACGT 2 cut(s) 1049, 1371
TaqI TCGA 4 cut(s) 78, 300, 534, 1318
TaqII GACCGA 1 cut(s) 1323
TasI AATT 8 cut(s) 360, 372, 425, 693, 986, 1080, 1145, 1323
TatI WGTACW 1 cut(s) 537
TauI GCSGC 6 cut(s) 61, 101, 104, 199, 283, 286
TfiI GAWTC 3 cut(s) 163, 1238, 1351
Tru1I TTAA 7 cut(s) 359, 383, 444, 480, 1008, 1079, 1136
Tru9I TTAA 7 cut(s) 359, 383, 444, 480, 1008, 1079, 1136
TscAI CASTG 2 cut(s) 1126, 1132
TseFI GTSAC 3 cut(s) 106, 496, 1304
TseI GCWGC 7 cut(s) 33, 66, 451, 864, 1018, 1032, 1130
Tsp45I GTSAC 3 cut(s) 106, 496, 1304
TspDTI ATGAA 3 cut(s) 418, 629, 717
TspGWI ACGGA 2 cut(s) 849, 925
TspRI CASTG 2 cut(s) 1126, 1132
VpaK11BI GGWCC 2 cut(s) 954, 1283
VspI ATTAAT 1 cut(s) 444
XapI RAATTY 2 cut(s) 425, 1145
XbaI TCTAGA 1 cut(s) 1241
XcmI CCANNNNNNNNNTGG 1 cut(s) 1210
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 5 cut(s) 351, 494, 563, 774, 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.