MD14G1150700.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014887
Physical Location & Seq
Forward (+)
24382135 .. 24386841
4707 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1150700.v1.1.491

Sequence Viewer

Length: 1704 bp
ATGGGGTGCGTTTTGGGCACGGAGGCATCGGCCGCCGGGAGTCAACGGAGTTCCGGTGAAGCTCGGCGCCGTCGCCGGAGCATCGAGGAGCCATCCGGCGCCACTGAGCGAGTCGAGGCCGTTAGGTCTGATAGCAAAGAGGTGGTAGCGAGGCAGAGGGCGAACAGAGCGAATCACACTGGTGATTTCCCGGCGATCGAGCGGCGGAAGCCTTTCCCGGCGAAACAGGAAGGTTGGCCGTCGTGGCTGCTGGCCGTGGCCGGCGATGCGATCCAGGGCTGGACCCCTCGGCGCGCCAACACGTTCGAGAAGCTGGCTAAGATTGGGCAAGGGACTTATAGCAATGTGTATAAAGCAAGAGACTTGATTAGTGGTAAAATTGTGGCATTAAAGAAGGTTAGGTTTGATAATTTGGAGCCAGAGAGTGTGAAATTCATGGCCAGAGAGATCAATGTGCTCAGAAAACTTGACCATCCCAATGTGATTAAGCTTGAAGGGTTAGTCACTTCAAGAATGTCCTGCAGCCTCTACTTGGTTTTCGAGTACATGGAGCATGATCTCTCGGGCCTCGCCGCCAGCTCCGGGGTGAAATTCACTGAGCCTCAGGTCAAGTGCTATATGAAACAACTGCTGTCTGGGCTTGAGCATTGCCACAACCATGGTGTCTTGCACCGGGATATCAAGGGTTCCAATCTGTTAATTGACGACGAAGGAATTTTAAAAATAGCTGATTTTGGACTGGCTACTTTTTTTGATCCTGAGAAGAGGCAACCCTTGACCAGTCGAGTTGTCACTCTCTGGTACCGCCCTCCTGAACTTCTTCTTGGTGCCACTTTCTACGGAGTTGGAGTTGACCTTTGGAGCGCGGGCTGCATTTTGGCAGAGTTACTTTCCGGGAAGCCTATTATGCCAGGACGGACGGAGGTTGAACAACTGCATAAGATATTTAAGTTATGTGGCTCCCCATCTGAGGAATACTGGAAAAAATATAAATTGCCAAATGCAACACTTTTTAAGCCACAGCAGCCATACAAGCGTTGTGTAGCGGAAACATTCAAGGATTTCCCACCTTTTTCGCTACCATTAGTTGAATCTCTTCTGTCACTAGATGCTGATGGACGAGGCACTGCCACCGCTGCTCTAAATAGTGAATTCTTTACTACCGAGCCTTTTGCTTGTGAGCCATCAAGTCTACCAAAATATCCGCCCGCCAAAGAAATGGATGTAAAGTTACGAGATGAAGAAGCTAGAAGGCAAAGAGGTCTCAGTGGGAAACCAAATGCTGCTGAAGGTCCCAGGAGAGTTAAAGCTCGTGATCGAGTTGGTCGGGCAATTCCAGCTCCAGAAGCTAATGCAGAGGCTCAAGGAAACTTAGATAGGTGGAGAGTGATGACACAAGCAAATGCCAAGAGCAAGAGTGAGAAATTCCCACCCCCACATCAGGATGGAGCCGTCGGATACCCACAAGAAGCATCACATAAAGGACCGCTCTCATTTCTCACGACGGATGCTTCATTTGGATCAATGATTTTGAACACAAAGTCTTCATCATCTGTTAAAAGCGTGGGAGCAGGCGGGGGCCCTTCAAGGAGGAAGAAAACAAATAGAGAAGACCCCTTGATGGCTCCACCTCGGAAGTTCATCCAGGCGTTCAAGCCTTCCTCGATAGGGCTATCGATGAATTTATTGTTTAAGAGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

568

Amino Acids

62.73

Weight (kDa)

9.58

Isoelectric Point (pI)

53.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 102 - 386 4e-64 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 105 - 315 1.7e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50860 AT5G50860
fragaria_vesca FvH4_5g09040
malus_domestica MD06G1134300.v1.1 MD14G1150700.v1.1
prunus_persica Prupe.5G145300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189181
rosa_laevigata RLG00000004626
rosa_multiflora Rmu_ssc0000251.1_g000001
rosa_roxburghii Rroxscaffold_3G00265730
rosa_rugosa Rorug06G0496600
rosa_samantha Rh7AG101200 Rh7BG103800 Rh7CG105200 Rh7DG103100
rosa_wichuraiana Rw7G008740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 801
AccB1I GGYRCC 4 cut(s) 66, 98, 801, 827
AccBSI CCGCTC 2 cut(s) 202, 1489
AccI GTMKAC 1 cut(s) 1192
AccII CGCG 2 cut(s) 294, 866
AclWI GGATC 3 cut(s) 265, 749, 1528
AcoI YGGCCR 5 cut(s) 30, 236, 252, 258, 438
AcsI RAATTY 6 cut(s) 431, 590, 714, 1151, 1424, 1681
AcuI CTGAAG 1 cut(s) 1308
AcyI GRCGYC 2 cut(s) 67, 99
AfaI GTAC 2 cut(s) 545, 803
AfiI CCNNNNNNNGG 6 cut(s) 532, 582, 970, 1441, 1621, 1668
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 8 cut(s) 494, 510, 929, 1057, 1091, 1534, 1587, 1654
AjnI CCWGG 4 cut(s) 273, 910, 1295, 1644
AjuI GAANNNNNNNTTGG 2 cut(s) 807, 839
AleI CACNNNNGTG 1 cut(s) 180
AluBI AGCT 9 cut(s) 62, 313, 490, 579, 728, 1247, 1310, 1340, 1349
AluI AGCT 9 cut(s) 62, 313, 490, 579, 728, 1247, 1310, 1340, 1349
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 2 cut(s) 354, 1268
AlwI GGATC 3 cut(s) 265, 749, 1528
Ama87I CYCGRG 1 cut(s) 562
AoxI GGCC 8 cut(s) 30, 117, 236, 252, 258, 438, 565, 1579
ApaI GGGCCC 1 cut(s) 1583
ApeKI GCWGC 6 cut(s) 247, 522, 870, 1024, 1136, 1283
ApoI RAATTY 6 cut(s) 431, 590, 714, 1151, 1424, 1681
AscI GGCGCGCC 1 cut(s) 292
Asp700I GAANNNNTTC 3 cut(s) 212, 819, 1095
Asp718I GGTACC 1 cut(s) 801
AspLEI GCGC 5 cut(s) 69, 101, 294, 296, 866
AspS9I GGNCC 6 cut(s) 282, 565, 1292, 1484, 1579, 1580
AsuC2I CCSGG 6 cut(s) 37, 191, 218, 583, 674, 895
AsuHPI GGTGA 3 cut(s) 68, 194, 598
AvaI CYCGRG 1 cut(s) 562
AvaII GGWCC 3 cut(s) 282, 1292, 1484
AxyI CCTNAGG 1 cut(s) 603
BaeGI GKGCMC 2 cut(s) 20, 1583
BalI TGGCCA 1 cut(s) 440
BanI GGYRCC 4 cut(s) 66, 98, 801, 827
BanII GRGCYC 1 cut(s) 1583
BauI CACGAG 1 cut(s) 1311
BbsI GAAGAC 2 cut(s) 1536, 1617
Bbv12I GWGCWC 1 cut(s) 459
BbvI GCAGC 6 cut(s) 234, 534, 857, 1036, 1123, 1270
BccI CCATC 7 cut(s) 100, 480, 973, 1109, 1192, 1439, 1615
BceAI ACGGC 5 cut(s) 54, 104, 223, 239, 1436
BcgI CGANNNNNNTGC 2 cut(s) 1154, 1188
BciT130I CCWGG 4 cut(s) 275, 912, 1297, 1646
BciVI GTATCC 1 cut(s) 1451
BcnI CCSGG 6 cut(s) 37, 191, 218, 583, 674, 895
BcoDI GTCTC 2 cut(s) 354, 1268
BfaI CTAG 2 cut(s) 1106, 1248
BfmI CTRYAG 1 cut(s) 520
BfoI RGCGCY 2 cut(s) 70, 102
BfuI GTATCC 1 cut(s) 1451
BglI GCCNNNNNGGC 1 cut(s) 244
BisI GCNGC 9 cut(s) 33, 203, 248, 523, 573, 871, 1025, 1137, 1284
BlsI GCNGC 9 cut(s) 34, 204, 249, 524, 574, 872, 1026, 1138, 1285
Bme18I GGWCC 3 cut(s) 282, 1292, 1484
BmeT110I CYCGRG 1 cut(s) 562
BmgT120I GGNCC 6 cut(s) 282, 565, 1292, 1484, 1579, 1580
BmsI GCATC 6 cut(s) 35, 90, 256, 1099, 1481, 1498
BpiI GAAGAC 2 cut(s) 1536, 1617
BpmI CTGGAG 1 cut(s) 1326
BpuEI CTTGAG 2 cut(s) 662, 1347
BpuMI CCSGG 6 cut(s) 37, 191, 218, 583, 674, 895
Bsa29I ATCGAT 1 cut(s) 1676
BsaHI GRCGYC 2 cut(s) 67, 99
BsaI GGTCTC 1 cut(s) 1268
BsaJI CCNNGG 7 cut(s) 255, 274, 287, 582, 658, 1295, 1631
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 834, 864
Bsc4I CCNNNNNNNGG 6 cut(s) 532, 582, 970, 1441, 1621, 1668
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 4 cut(s) 184, 744, 780, 983
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 2 cut(s) 349, 646
BseBI CCWGG 4 cut(s) 275, 912, 1297, 1646
BseCI ATCGAT 1 cut(s) 1676
BseDI CCNNGG 7 cut(s) 255, 274, 287, 582, 658, 1295, 1631
BseGI GGATG 6 cut(s) 92, 472, 1228, 1450, 1513, 1641
BseLI CCNNNNNNNGG 6 cut(s) 532, 582, 970, 1441, 1621, 1668
BseMI GCAATG 2 cut(s) 349, 646
BseMII CTCAG 7 cut(s) 96, 472, 588, 617, 750, 960, 1279
BseNI ACTGG 4 cut(s) 184, 744, 780, 983
BsePI GCGCGC 1 cut(s) 292
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 2 cut(s) 20, 1583
BseX3I CGGCCG 1 cut(s) 30
BseXI GCAGC 6 cut(s) 234, 534, 857, 1036, 1123, 1270
Bsh1236I CGCG 2 cut(s) 294, 866
Bsh1285I CGRYCG 2 cut(s) 33, 198
BshFI GGCC 8 cut(s) 32, 119, 238, 254, 260, 440, 567, 1581
BshNI GGYRCC 4 cut(s) 66, 98, 801, 827
BshVI ATCGAT 1 cut(s) 1676
BsiEI CGRYCG 2 cut(s) 33, 198
BsiHKAI GWGCWC 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 562
BslFI GGGAC 2 cut(s) 346, 1278
BslI CCNNNNNNNGG 6 cut(s) 532, 582, 970, 1441, 1621, 1668
BsmAI GTCTC 2 cut(s) 354, 1268
BsmFI GGGAC 2 cut(s) 346, 1278
BsnI GGCC 8 cut(s) 32, 119, 238, 254, 260, 440, 567, 1581
Bso31I GGTCTC 1 cut(s) 1268
BsoBI CYCGRG 1 cut(s) 562
Bsp120I GGGCCC 1 cut(s) 1579
Bsp1286I GDGCHC 3 cut(s) 20, 459, 1583
Bsp143I GATC 7 cut(s) 195, 270, 447, 556, 754, 1315, 1520
Bsp19I CCATGG 1 cut(s) 658
BspANI GGCC 8 cut(s) 32, 119, 238, 254, 260, 440, 567, 1581
BspCNI CTCAG 7 cut(s) 97, 471, 589, 616, 751, 961, 1278
BspDI ATCGAT 1 cut(s) 1676
BspFNI CGCG 2 cut(s) 294, 866
BspMAI CTGCAG 1 cut(s) 524
BspPI GGATC 3 cut(s) 265, 749, 1528
BspT107I GGYRCC 4 cut(s) 66, 98, 801, 827
BspTNI GGTCTC 1 cut(s) 1268
BsrBI CCGCTC 2 cut(s) 202, 1489
BsrDI GCAATG 2 cut(s) 349, 646
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 4 cut(s) 184, 744, 780, 983
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 7 cut(s) 255, 274, 287, 582, 658, 1295, 1631
BssHII GCGCGC 1 cut(s) 292
BssMI GATC 7 cut(s) 195, 270, 447, 556, 754, 1315, 1520
BssNI GRCGYC 2 cut(s) 67, 99
BssSI CACGAG 1 cut(s) 1311
BssT1I CCWWGG 1 cut(s) 658
Bst2BI CACGAG 1 cut(s) 1311
Bst2UI CCWGG 4 cut(s) 275, 912, 1297, 1646
Bst6I CTCTTC 2 cut(s) 758, 1101
BstACI GRCGYC 2 cut(s) 67, 99
BstC8I GCNNGC 8 cut(s) 252, 262, 294, 315, 577, 868, 1209, 1573
BstDEI CTNAG 9 cut(s) 105, 318, 458, 597, 603, 759, 969, 1265, 1372
BstDSI CCRYGG 2 cut(s) 255, 658
BstF5I GGATG 6 cut(s) 92, 472, 1228, 1450, 1513, 1641
BstFNI CGCG 2 cut(s) 294, 866
BstH2I RGCGCY 2 cut(s) 70, 102
BstHHI GCGC 5 cut(s) 69, 101, 294, 296, 866
BstKTI GATC 7 cut(s) 198, 273, 450, 559, 757, 1318, 1523
BstMAI GTCTC 2 cut(s) 354, 1268
BstMBI GATC 7 cut(s) 195, 270, 447, 556, 754, 1315, 1520
BstMCI CGRYCG 2 cut(s) 33, 198
BstNI CCWGG 4 cut(s) 275, 912, 1297, 1646
BstSFI CTRYAG 1 cut(s) 520
BstSLI GKGCMC 2 cut(s) 20, 1583
BstUI CGCG 2 cut(s) 294, 866
BstV1I GCAGC 6 cut(s) 234, 534, 857, 1036, 1123, 1270
BstV2I GAAGAC 2 cut(s) 1536, 1617
BstXI CCANNNNNNTGG 2 cut(s) 659, 1219
BstZI CGGCCG 1 cut(s) 30
Bsu15I ATCGAT 1 cut(s) 1676
Bsu36I CCTNAGG 1 cut(s) 603
BsuI GTATCC 1 cut(s) 1451
BsuRI GGCC 8 cut(s) 32, 119, 238, 254, 260, 440, 567, 1581
BsuTUI ATCGAT 1 cut(s) 1676
BtgI CCRYGG 2 cut(s) 255, 658
BtgZI GCGATG 1 cut(s) 279
BtsCI GGATG 6 cut(s) 92, 472, 1228, 1450, 1513, 1641
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 5 cut(s) 102, 177, 594, 1125, 1273
Cac8I GCNNGC 8 cut(s) 252, 262, 294, 315, 577, 868, 1209, 1573
CfoI GCGC 5 cut(s) 69, 101, 294, 296, 866
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 6 cut(s) 282, 565, 1292, 1484, 1579, 1580
ClaI ATCGAT 1 cut(s) 1676
Csp6I GTAC 2 cut(s) 544, 802
CviAII CATG 4 cut(s) 436, 547, 554, 659
CviQI GTAC 2 cut(s) 544, 802
DdeI CTNAG 9 cut(s) 105, 318, 458, 597, 603, 759, 969, 1265, 1372
DinI GGCGCC 2 cut(s) 68, 100
DpnI GATC 7 cut(s) 197, 272, 449, 558, 756, 1317, 1522
DpnII GATC 7 cut(s) 195, 270, 447, 556, 754, 1315, 1520
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 5 cut(s) 30, 236, 252, 258, 438
EagI CGGCCG 1 cut(s) 30
Eam1104I CTCTTC 2 cut(s) 758, 1101
EarI CTCTTC 2 cut(s) 758, 1101
EciI GGCGGA 2 cut(s) 220, 1194
EclXI CGGCCG 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 658
Eco24I GRGCYC 1 cut(s) 1583
Eco31I GGTCTC 1 cut(s) 1268
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 3 cut(s) 282, 1292, 1484
Eco52I CGGCCG 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 1308
Eco81I CCTNAGG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 562
EcoO109I RGGNCCY 3 cut(s) 1292, 1579, 1580
EcoRI GAATTC 1 cut(s) 1151
EcoRII CCWGG 4 cut(s) 273, 910, 1295, 1644
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 1583
EgeI GGCGCC 2 cut(s) 68, 100
EheI GGCGCC 2 cut(s) 68, 100
ErhI CCWWGG 1 cut(s) 658
FaeI CATG 4 cut(s) 439, 550, 557, 662
FaqI GGGAC 2 cut(s) 346, 1278
FatI CATG 4 cut(s) 435, 546, 553, 658
FauI CCCGC 3 cut(s) 859, 1216, 1568
FblI GTMKAC 1 cut(s) 1192
Fnu4HI GCNGC 9 cut(s) 33, 203, 248, 523, 573, 871, 1025, 1137, 1284
FokI GGATG 6 cut(s) 79, 459, 1235, 1457, 1520, 1628
FriOI GRGCYC 1 cut(s) 1583
Fsp4HI GCNGC 9 cut(s) 33, 203, 248, 523, 573, 871, 1025, 1137, 1284
FspBI CTAG 2 cut(s) 1106, 1248
GlaI GCGC 5 cut(s) 68, 100, 293, 295, 865
GluI GCNGC 9 cut(s) 33, 203, 248, 523, 573, 871, 1025, 1137, 1284
GsuI CTGGAG 1 cut(s) 1326
HaeII RGCGCY 2 cut(s) 70, 102
HaeIII GGCC 8 cut(s) 32, 119, 238, 254, 260, 440, 567, 1581
HhaI GCGC 5 cut(s) 69, 101, 294, 296, 866
Hin1I GRCGYC 2 cut(s) 67, 99
Hin1II CATG 4 cut(s) 439, 550, 557, 662
Hin6I GCGC 5 cut(s) 67, 99, 292, 294, 864
HinP1I GCGC 5 cut(s) 67, 99, 292, 294, 864
HincII GTYRAC 2 cut(s) 44, 853
HindII GTYRAC 2 cut(s) 44, 853
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 4 cut(s) 40, 111, 172, 1091
HphI GGTGA 3 cut(s) 68, 194, 598
Hpy166II GTNNAC 3 cut(s) 44, 853, 1193
Hpy188I TCNGA 5 cut(s) 130, 461, 970, 1457, 1635
Hpy188III TCNNGA 8 cut(s) 307, 510, 758, 812, 1313, 1343, 1442, 1501
Hpy8I GTNNAC 3 cut(s) 44, 853, 1193
Hpy99I CGWCG 5 cut(s) 75, 244, 710, 1457, 1507
HpyAV CCTTC 8 cut(s) 224, 388, 488, 704, 1245, 1283, 1593, 1668
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 6 cut(s) 522, 670, 873, 937, 1004, 1355
HpyF3I CTNAG 9 cut(s) 105, 318, 458, 597, 603, 759, 969, 1265, 1372
HpySE526I ACGT 1 cut(s) 302
Hsp92I GRCGYC 2 cut(s) 67, 99
Hsp92II CATG 4 cut(s) 439, 550, 557, 662
HspAI GCGC 5 cut(s) 67, 99, 292, 294, 864
KasI GGCGCC 2 cut(s) 66, 98
KpnI GGTACC 1 cut(s) 805
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Kzo9I GATC 7 cut(s) 195, 270, 447, 556, 754, 1315, 1520
Lsp1109I GCAGC 6 cut(s) 234, 534, 857, 1036, 1123, 1270
LweI GCATC 6 cut(s) 35, 90, 256, 1099, 1481, 1498
MaeI CTAG 2 cut(s) 1106, 1248
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 5 cut(s) 502, 790, 885, 1101, 1230
MalI GATC 7 cut(s) 197, 272, 449, 558, 756, 1317, 1522
MbiI CCGCTC 2 cut(s) 202, 1489
MboI GATC 7 cut(s) 195, 270, 447, 556, 754, 1315, 1520
MboII GAAGA 7 cut(s) 775, 812, 1088, 1253, 1536, 1606, 1622
MhlI GDGCHC 3 cut(s) 20, 459, 1583
MlsI TGGCCA 1 cut(s) 440
MluNI TGGCCA 1 cut(s) 440
Mly113I GGCGCC 2 cut(s) 67, 99
MlyI GAGTC 2 cut(s) 49, 120
MmeI TCCRAC 2 cut(s) 826, 1435
Mox20I TGGCCA 1 cut(s) 440
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 3 cut(s) 212, 819, 1095
MscI TGGCCA 1 cut(s) 440
MseI TTAA 9 cut(s) 389, 486, 698, 719, 948, 1014, 1305, 1557, 1692
MslI CAYNNNNRTG 4 cut(s) 180, 477, 657, 1443
Msp20I TGGCCA 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 1136
MvaI CCWGG 4 cut(s) 275, 912, 1297, 1646
MvnI CGCG 2 cut(s) 294, 866
NaeI GCCGGC 1 cut(s) 262
NarI GGCGCC 2 cut(s) 67, 99
NciI CCSGG 6 cut(s) 37, 191, 218, 583, 674, 895
NcoI CCATGG 1 cut(s) 658
NdeII GATC 7 cut(s) 195, 270, 447, 556, 754, 1315, 1520
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 4 cut(s) 439, 550, 557, 662
NmeAIII GCCGAG 2 cut(s) 43, 268
NmuCI GTSAC 3 cut(s) 502, 790, 1101
OliI CACNNNNGTG 1 cut(s) 180
PalAI GGCGCGCC 1 cut(s) 292
PauI GCGCGC 1 cut(s) 292
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 3 cut(s) 212, 819, 1095
PfeI GAWTC 2 cut(s) 172, 1091
PfoI TCCNGGA 1 cut(s) 893
PkrI GCNGC 9 cut(s) 34, 204, 249, 524, 574, 872, 1026, 1138, 1285
Ple19I CGATCG 1 cut(s) 198
PleI GAGTC 2 cut(s) 48, 119
PluTI GGCGCC 2 cut(s) 70, 102
PpsI GAGTC 2 cut(s) 48, 119
PpuMI RGGWCCY 1 cut(s) 1292
Psp5II RGGWCCY 1 cut(s) 1292
Psp6I CCWGG 4 cut(s) 273, 910, 1295, 1644
PspGI CCWGG 4 cut(s) 273, 910, 1295, 1644
PspOMI GGGCCC 1 cut(s) 1579
PspPI GGNCC 6 cut(s) 282, 565, 1292, 1484, 1579, 1580
PspPPI RGGWCCY 1 cut(s) 1292
PstI CTGCAG 1 cut(s) 524
PteI GCGCGC 1 cut(s) 292
PvuI CGATCG 1 cut(s) 198
RsaI GTAC 2 cut(s) 545, 803
RsaNI GTAC 2 cut(s) 544, 802
RseI CAYNNNNRTG 4 cut(s) 180, 477, 657, 1443
SaqAI TTAA 9 cut(s) 389, 486, 698, 719, 948, 1014, 1305, 1557, 1692
SatI GCNGC 9 cut(s) 33, 203, 248, 523, 573, 871, 1025, 1137, 1284
Sau3AI GATC 7 cut(s) 195, 270, 447, 556, 754, 1315, 1520
Sau96I GGNCC 6 cut(s) 282, 565, 1292, 1484, 1579, 1580
SchI GAGTC 2 cut(s) 49, 120
SduI GDGCHC 3 cut(s) 20, 459, 1583
SfaNI GCATC 6 cut(s) 35, 90, 256, 1099, 1481, 1498
SfcI CTRYAG 1 cut(s) 520
SfoI GGCGCC 2 cut(s) 68, 100
SgsI GGCGCGCC 1 cut(s) 292
SinI GGWCC 3 cut(s) 282, 1292, 1484
SmiMI CAYNNNNRTG 4 cut(s) 180, 477, 657, 1443
SmlI CTYRAG 2 cut(s) 641, 1362
SmoI CTYRAG 2 cut(s) 641, 1362
SspDI GGCGCC 2 cut(s) 66, 98
SspMI CTAG 2 cut(s) 1106, 1248
StyI CCWWGG 1 cut(s) 658
TaiI ACGT 1 cut(s) 305
TaqI TCGA 9 cut(s) 84, 114, 198, 306, 540, 784, 1318, 1664, 1676
TatI WGTACW 1 cut(s) 543
TauI GCSGC 3 cut(s) 35, 205, 575
TfiI GAWTC 2 cut(s) 172, 1091
Tru1I TTAA 9 cut(s) 389, 486, 698, 719, 948, 1014, 1305, 1557, 1692
Tru9I TTAA 9 cut(s) 389, 486, 698, 719, 948, 1014, 1305, 1557, 1692
TscAI CASTG 5 cut(s) 109, 184, 601, 1132, 1273
TseFI GTSAC 3 cut(s) 502, 790, 1101
TseI GCWGC 6 cut(s) 247, 522, 870, 1024, 1136, 1283
Tsp45I GTSAC 3 cut(s) 502, 790, 1101
TspDTI ATGAA 7 cut(s) 424, 635, 1254, 1503, 1536, 1630, 1694
TspGWI ACGGA 6 cut(s) 35, 61, 855, 931, 935, 1520
TspRI CASTG 5 cut(s) 109, 184, 601, 1132, 1273
VpaK11BI GGWCC 3 cut(s) 282, 1292, 1484
XapI RAATTY 6 cut(s) 431, 590, 714, 1151, 1424, 1681
XmiI GTMKAC 1 cut(s) 1192
XmnI GAANNNNTTC 3 cut(s) 212, 819, 1095
XspI CTAG 2 cut(s) 1106, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.