Rw7G008740

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014887
Physical Location & Seq
Forward (+)
8038924 .. 8043560
4637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G008740.1

Sequence Viewer

Length: 1269 bp
ATGGCCAGAGAGATTAATGTGCTGCGGAAACTTGACCATCCCAATGTGGTTAAGCTTGAAGGTCTAGTCACTTCAAGAATGTCATGTAGTCTCTACTTGGTTTTCGAGTACATGGAGCATGATCTCTCCGGCCTAGCCGCCTCCACGGGGATCAAGTTCACCGAGTCTCAGGTCAAGTGCTATATGAAACAGTTGCTGTCTGGCCTTGAACATTGCCACATCCACGGAGTCTTGCACCGTGATATCAAGGGGTCTAATCTACTAATTGACAATGAAGGAGTTCTGAAAATAGCTGATTTTGGATTGGCTACATTTTTTGATCCTCAACAGAAGCAACCCTTGACTAGTCGGGTTGTTACTCTCTGGTACCGCCCCCCTGAGCTTCTTCTTGGGGCCACTTGTTACGGAGTTGGTGTTGACCTTTGGAGTGCTGGCTGCATTTTGGCAGAGTTGCTTTCCGGGAAGCCTATTATGCCAGGACGGACAGAGGTTGAACAACTGCATAAGATATTCAAGTTATGTGGGTCCCCATCTGAGGATTACTGGAAAAAATACAAATTGCCAAATGCAACACTTTTTAAACCACAGCAGCCATACAAACGCTGCTTGGCAGAAACGTTCAAGGACTTCCCACCTTCTTCCCTACCTTTAATCGAGTCTCTTCTCTCAATAGACTCTGAAGGACGAGGCACTGCCACTGCTGCTTTAAATAGTGAATTCTTTACTACTGAGCCTTTTGCTTGTGAGCCATCAAGTTTACCAAAATATCCTCCCAGCAAGGAAATGGATGTAAAGTTACGAGATGCAGAATCTAGAAGGCAAAGAGGTCTAAGTGGGAAGACCAATGCTGATGGTCCAAAAAAAGTCAAAGCTCGTGACCGAGCTGGTCGAGCAATTCCAGTTCCAGAAGCTAATGCAGAGAATCAAGGAAACTTAGACAGGTGGAGAGCTATGACACAAACAAACACCAAGAGCAAGAGTGAGAAATTCCCACCCCCACATCAGGATGGAGCTGTTGGATACCCCCAAGAAGCATCACATAAAGGCCCTCTATCATTTGTCACAAGCGATGCTTCATTCGGATCAGTGGTTTTCAACACAAAGTCTTCAGGATCTGTTAAAAGCATGGGAGCGGCTGGGGGGTCTTCAAGGCGGAGGAAAAACAACAGAGAAAACCCTACGAGGGCTCCTTCCCGGAAATTCATCCAGGCGTTCAAGCCCTCCTCGATAGGGCTATCAATGAATCTATTATTTAGCCGTAGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

422

Amino Acids

46.64

Weight (kDa)

9.47

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 2 - 241 1.5e-54 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 2 - 170 1.3e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50860 AT5G50860
fragaria_vesca FvH4_5g09040
malus_domestica MD06G1134300.v1.1 MD14G1150700.v1.1
prunus_persica Prupe.5G145300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189181
rosa_laevigata RLG00000004626
rosa_multiflora Rmu_ssc0000251.1_g000001
rosa_roxburghii Rroxscaffold_3G00265730
rosa_rugosa Rorug06G0496600
rosa_samantha Rh7AG101200 Rh7BG103800 Rh7CG105200 Rh7DG103100
rosa_wichuraiana Rw7G008740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 366
AccB1I GGYRCC 1 cut(s) 366
AccBSI CCGCTC 1 cut(s) 1133
AciI CCGC 5 cut(s) 25, 138, 370, 1133, 1153
AclI AACGTT 1 cut(s) 617
AclWI GGATC 4 cut(s) 158, 314, 1090, 1120
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 716, 986, 1199
AcuI CTGAAG 2 cut(s) 699, 1092
AfaI GTAC 2 cut(s) 110, 368
AfiI CCNNNNNNNGG 5 cut(s) 147, 535, 1003, 1183, 1230
AgsI TTSAA 9 cut(s) 59, 75, 209, 494, 514, 622, 1096, 1149, 1216
AhlI ACTAGT 1 cut(s) 344
AjnI CCWGG 2 cut(s) 475, 1206
AluBI AGCT 8 cut(s) 55, 293, 382, 872, 884, 911, 950, 1013
AluI AGCT 8 cut(s) 55, 293, 382, 872, 884, 911, 950, 1013
Alw26I GTCTC 3 cut(s) 95, 171, 663
AlwI GGATC 4 cut(s) 158, 314, 1090, 1120
AlwNI CAGNNNCTG 2 cut(s) 196, 1115
AoxI GGCC 5 cut(s) 3, 130, 202, 393, 1045
ApeKI GCWGC 5 cut(s) 22, 435, 589, 603, 701
ApoI RAATTY 3 cut(s) 716, 986, 1199
AseI ATTAAT 1 cut(s) 15
Asp700I GAANNNNTTC 1 cut(s) 279
Asp718I GGTACC 1 cut(s) 366
AspS9I GGNCC 4 cut(s) 393, 525, 854, 1046
AsuC2I CCSGG 2 cut(s) 460, 1195
AsuHPI GGTGA 1 cut(s) 151
AvaII GGWCC 2 cut(s) 525, 854
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 366
BanII GRGCYC 1 cut(s) 1189
BauI CACGAG 1 cut(s) 873
BbsI GAAGAC 3 cut(s) 845, 1098, 1137
BbvI GCAGC 5 cut(s) 9, 422, 590, 601, 688
BccI CCATC 5 cut(s) 45, 538, 757, 845, 1001
BceAI ACGGC 1 cut(s) 1242
BciT130I CCWGG 2 cut(s) 477, 1208
BciVI GTATCC 1 cut(s) 1013
BcnI CCSGG 2 cut(s) 460, 1195
BcoDI GTCTC 3 cut(s) 95, 171, 663
BcuI ACTAGT 1 cut(s) 344
BfaI CTAG 4 cut(s) 65, 134, 345, 813
BfuI GTATCC 1 cut(s) 1013
BisI GCNGC 7 cut(s) 23, 138, 436, 590, 604, 702, 1134
BlsI GCNGC 7 cut(s) 24, 139, 437, 591, 605, 703, 1135
Bme1390I CCNGG 4 cut(s) 460, 477, 1195, 1208
Bme18I GGWCC 2 cut(s) 525, 854
BmgT120I GGNCC 4 cut(s) 393, 525, 854, 1046
BmiI GGNNCC 5 cut(s) 368, 394, 526, 527, 1188
BmrFI CCNGG 4 cut(s) 460, 477, 1195, 1208
BmsI GCATC 3 cut(s) 793, 1043, 1060
BpiI GAAGAC 3 cut(s) 845, 1098, 1137
Bpu10I CCTNAGC 1 cut(s) 378
BpuMI CCSGG 2 cut(s) 460, 1195
BsaJI CCNNGG 2 cut(s) 144, 223
BsaXI ACNNNNNCTCC 4 cut(s) 399, 429, 1171, 1201
Bsc4I CCNNNNNNNGG 5 cut(s) 147, 535, 1003, 1183, 1230
Bse1I ACTGG 2 cut(s) 548, 899
Bse3DI GCAATG 1 cut(s) 211
BseBI CCWGG 2 cut(s) 477, 1208
BseDI CCNNGG 2 cut(s) 144, 223
BseGI GGATG 5 cut(s) 37, 219, 793, 1012, 1203
BseLI CCNNNNNNNGG 5 cut(s) 147, 535, 1003, 1183, 1230
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 4 cut(s) 182, 369, 525, 720
BseNI ACTGG 2 cut(s) 548, 899
BseRI GAGGAG 1 cut(s) 1213
BseXI GCAGC 5 cut(s) 9, 422, 590, 601, 688
BseYI CCCAGC 2 cut(s) 773, 1136
BshFI GGCC 5 cut(s) 5, 132, 204, 395, 1047
BshNI GGYRCC 1 cut(s) 366
BsiSI CCGG 3 cut(s) 129, 459, 1195
BslFI GGGAC 1 cut(s) 511
BslI CCNNNNNNNGG 5 cut(s) 147, 535, 1003, 1183, 1230
BsmAI GTCTC 3 cut(s) 95, 171, 663
BsmFI GGGAC 1 cut(s) 511
BsnI GGCC 5 cut(s) 5, 132, 204, 395, 1047
Bsp1286I GDGCHC 1 cut(s) 1189
Bsp143I GATC 5 cut(s) 121, 150, 319, 1082, 1112
BspACI CCGC 5 cut(s) 25, 138, 370, 1133, 1153
BspANI GGCC 5 cut(s) 5, 132, 204, 395, 1047
BspCNI CTCAG 4 cut(s) 181, 370, 526, 721
BspLI GGNNCC 5 cut(s) 368, 394, 526, 527, 1188
BspPI GGATC 4 cut(s) 158, 314, 1090, 1120
BspT107I GGYRCC 1 cut(s) 366
BsrBI CCGCTC 1 cut(s) 1133
BsrDI GCAATG 1 cut(s) 211
BsrI ACTGG 2 cut(s) 548, 899
BssECI CCNNGG 2 cut(s) 144, 223
BssMI GATC 5 cut(s) 121, 150, 319, 1082, 1112
BssSI CACGAG 1 cut(s) 873
Bst2BI CACGAG 1 cut(s) 873
Bst2UI CCWGG 2 cut(s) 477, 1208
Bst4CI ACNGT 2 cut(s) 192, 239
Bst6I CTCTTC 1 cut(s) 666
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 6 cut(s) 168, 378, 534, 729, 830, 934
BstDSI CCRYGG 2 cut(s) 144, 223
BstF5I GGATG 5 cut(s) 37, 219, 793, 1012, 1203
BstKTI GATC 5 cut(s) 124, 153, 322, 1085, 1115
BstMAI GTCTC 3 cut(s) 95, 171, 663
BstMBI GATC 5 cut(s) 121, 150, 319, 1082, 1112
BstMWI GCNNNNNNNGC 3 cut(s) 472, 701, 890
BstNI CCWGG 2 cut(s) 477, 1208
BstSCI CCNGG 4 cut(s) 458, 475, 1193, 1206
BstV1I GCAGC 5 cut(s) 9, 422, 590, 601, 688
BstV2I GAAGAC 3 cut(s) 845, 1098, 1137
BstX2I RGATCY 1 cut(s) 1112
BstYI RGATCY 1 cut(s) 1112
BsuI GTATCC 1 cut(s) 1013
BsuRI GGCC 5 cut(s) 5, 132, 204, 395, 1047
BtgI CCRYGG 2 cut(s) 144, 223
BtgZI GCGATG 1 cut(s) 1083
BtsCI GGATG 5 cut(s) 37, 219, 793, 1012, 1203
BtsI GCAGTG 2 cut(s) 690, 696
BtsIMutI CAGTG 3 cut(s) 690, 696, 1092
Cac8I GCNNGC 1 cut(s) 433
CaiI CAGNNNCTG 2 cut(s) 196, 1115
Cfr13I GGNCC 4 cut(s) 393, 525, 854, 1046
Csp6I GTAC 2 cut(s) 109, 367
CspCI CAANNNNNGTGG 2 cut(s) 502, 537
CviAII CATG 4 cut(s) 84, 112, 119, 1126
CviQI GTAC 2 cut(s) 109, 367
DdeI CTNAG 6 cut(s) 168, 378, 534, 729, 830, 934
DpnI GATC 5 cut(s) 123, 152, 321, 1084, 1114
DpnII GATC 5 cut(s) 121, 150, 319, 1082, 1112
DraI TTTAAA 2 cut(s) 580, 708
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 666
EarI CTCTTC 1 cut(s) 666
EciI GGCGGA 1 cut(s) 1168
Eco24I GRGCYC 1 cut(s) 1189
Eco32I GATATC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 525, 854
Eco57I CTGAAG 2 cut(s) 699, 1092
EcoO109I RGGNCCY 2 cut(s) 525, 1046
EcoRI GAATTC 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 475, 1206
EcoRV GATATC 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 1189
FaeI CATG 4 cut(s) 87, 115, 122, 1129
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 1057, 1089
FaqI GGGAC 1 cut(s) 511
FatI CATG 4 cut(s) 83, 111, 118, 1125
Fnu4HI GCNGC 7 cut(s) 23, 138, 436, 590, 604, 702, 1134
FokI GGATG 5 cut(s) 24, 206, 800, 1019, 1190
FriOI GRGCYC 1 cut(s) 1189
Fsp4HI GCNGC 7 cut(s) 23, 138, 436, 590, 604, 702, 1134
FspBI CTAG 4 cut(s) 65, 134, 345, 813
GluI GCNGC 7 cut(s) 23, 138, 436, 590, 604, 702, 1134
GsaI CCCAGC 2 cut(s) 777, 1140
HaeIII GGCC 5 cut(s) 5, 132, 204, 395, 1047
HapII CCGG 3 cut(s) 129, 459, 1195
Hin1II CATG 4 cut(s) 87, 115, 122, 1129
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 7 cut(s) 164, 228, 656, 674, 809, 922, 1243
HpaII CCGG 3 cut(s) 129, 459, 1195
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 3 cut(s) 159, 418, 758
Hpy188I TCNGA 4 cut(s) 285, 535, 679, 1082
Hpy188III TCNNGA 6 cut(s) 75, 813, 875, 905, 1004, 1110
Hpy8I GTNNAC 3 cut(s) 159, 418, 758
HpyAV CCTTC 6 cut(s) 53, 269, 645, 674, 810, 1200
HpyCH4III ACNGT 2 cut(s) 192, 239
HpyCH4IV ACGT 1 cut(s) 617
HpyCH4V TGCA 6 cut(s) 235, 438, 502, 569, 806, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 472, 701, 890
HpyF3I CTNAG 6 cut(s) 168, 378, 534, 729, 830, 934
HpySE526I ACGT 1 cut(s) 617
Hsp92II CATG 4 cut(s) 87, 115, 122, 1129
KflI GGGWCCC 1 cut(s) 525
KpnI GGTACC 1 cut(s) 370
Kzo9I GATC 5 cut(s) 121, 150, 319, 1082, 1112
LmnI GCTCC 4 cut(s) 115, 1010, 1130, 1192
Lsp1109I GCAGC 5 cut(s) 9, 422, 590, 601, 688
LweI GCATC 3 cut(s) 793, 1043, 1060
MaeI CTAG 4 cut(s) 65, 134, 345, 813
MaeII ACGT 1 cut(s) 617
MaeIII GTNAC 6 cut(s) 67, 355, 401, 795, 875, 1060
MalI GATC 5 cut(s) 123, 152, 321, 1084, 1114
MbiI CCGCTC 1 cut(s) 1133
MboI GATC 5 cut(s) 121, 150, 319, 1082, 1112
MboII GAAGA 6 cut(s) 377, 630, 653, 850, 1098, 1137
MflI RGATCY 1 cut(s) 1112
MhlI GDGCHC 1 cut(s) 1189
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 264, 557, 716, 894, 986, 1199
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 173, 237, 665, 668
MmeI TCCRAC 1 cut(s) 997
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 279
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 15, 51, 579, 650, 707, 1119
MslI CAYNNNNRTG 2 cut(s) 42, 1005
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 129, 459, 1195
MspR9I CCNGG 4 cut(s) 460, 477, 1195, 1208
MvaI CCWGG 2 cut(s) 477, 1208
MwoI GCNNNNNNNGC 3 cut(s) 472, 701, 890
NciI CCSGG 2 cut(s) 460, 1195
NdeII GATC 5 cut(s) 121, 150, 319, 1082, 1112
NlaIII CATG 4 cut(s) 87, 115, 122, 1129
NlaIV GGNNCC 5 cut(s) 368, 394, 526, 527, 1188
NmuCI GTSAC 3 cut(s) 67, 875, 1060
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 3 cut(s) 809, 922, 1243
PfoI TCCNGGA 2 cut(s) 458, 1193
PkrI GCNGC 7 cut(s) 24, 139, 437, 591, 605, 703, 1135
PleI GAGTC 4 cut(s) 172, 236, 664, 668
PpsI GAGTC 4 cut(s) 172, 236, 664, 668
PpuMI RGGWCCY 1 cut(s) 525
PshBI ATTAAT 1 cut(s) 15
Psp1406I AACGTT 1 cut(s) 617
Psp5II RGGWCCY 1 cut(s) 525
Psp6I CCWGG 2 cut(s) 475, 1206
PspFI CCCAGC 2 cut(s) 773, 1136
PspGI CCWGG 2 cut(s) 475, 1206
PspN4I GGNNCC 5 cut(s) 368, 394, 526, 527, 1188
PspPI GGNCC 4 cut(s) 393, 525, 854, 1046
PspPPI RGGWCCY 1 cut(s) 525
PstNI CAGNNNCTG 2 cut(s) 196, 1115
PsuI RGATCY 1 cut(s) 1112
RsaI GTAC 2 cut(s) 110, 368
RsaNI GTAC 2 cut(s) 109, 367
RseI CAYNNNNRTG 2 cut(s) 42, 1005
SaqAI TTAA 6 cut(s) 15, 51, 579, 650, 707, 1119
SatI GCNGC 7 cut(s) 23, 138, 436, 590, 604, 702, 1134
Sau3AI GATC 5 cut(s) 121, 150, 319, 1082, 1112
Sau96I GGNCC 4 cut(s) 393, 525, 854, 1046
SchI GAGTC 4 cut(s) 173, 237, 665, 668
ScrFI CCNGG 4 cut(s) 460, 477, 1195, 1208
SduI GDGCHC 1 cut(s) 1189
SfaNI GCATC 3 cut(s) 793, 1043, 1060
SinI GGWCC 2 cut(s) 525, 854
SmiMI CAYNNNNRTG 2 cut(s) 42, 1005
SpeI ACTAGT 1 cut(s) 344
Sse9I AATT 6 cut(s) 264, 557, 716, 894, 986, 1199
SsiI CCGC 5 cut(s) 25, 138, 370, 1133, 1153
SspMI CTAG 4 cut(s) 65, 134, 345, 813
StyD4I CCNGG 4 cut(s) 458, 475, 1193, 1206
TaaI ACNGT 2 cut(s) 192, 239
TaiI ACGT 1 cut(s) 620
TaqI TCGA 4 cut(s) 105, 654, 889, 1226
TaqII GACCGA 1 cut(s) 894
TasI AATT 6 cut(s) 264, 557, 716, 894, 986, 1199
TatI WGTACW 1 cut(s) 108
TauI GCSGC 2 cut(s) 140, 1136
TfiI GAWTC 3 cut(s) 809, 922, 1243
Tru1I TTAA 6 cut(s) 15, 51, 579, 650, 707, 1119
Tru9I TTAA 6 cut(s) 15, 51, 579, 650, 707, 1119
TscAI CASTG 3 cut(s) 697, 703, 1092
TseFI GTSAC 3 cut(s) 67, 875, 1060
TseI GCWGC 5 cut(s) 22, 435, 589, 603, 701
Tsp45I GTSAC 3 cut(s) 67, 875, 1060
TspDTI ATGAA 5 cut(s) 200, 288, 1065, 1192, 1256
TspGWI ACGGA 3 cut(s) 240, 420, 496
TspRI CASTG 3 cut(s) 697, 703, 1092
VpaK11BI GGWCC 2 cut(s) 525, 854
VspI ATTAAT 1 cut(s) 15
XapI RAATTY 3 cut(s) 716, 986, 1199
XbaI TCTAGA 1 cut(s) 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 781
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 4 cut(s) 65, 134, 345, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.