Rmu_ssc0000251.1_g000001

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000251.1
OG0014887
Physical Location & Seq
Forward (+)
1 .. 4192
4192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000251.1_g000001.1.cds

Sequence Viewer

Length: 1602 bp
gccgtcgccgtcgacggcggaggggttgttagggagaggaaggaagcggtggagaggcagagagtgaatcatcacaccggcgactttccggtggttgagcggcggaagcagtttcaggtgaaccagcaaggctggccgtcgtggctattggccgtcgccggcgacgcgatccagggatggacgccgcggcgggccaacactttcgagaagctttcaaagattgggcaagggacttatagcaatgtatataaagccagagacttaatttctgggaaaattgtggcgttaaagaaggttagatttgataacttggagccggagagtgtgaaattcatggccagagagattaatgtgctgcggaaacttgaccatcccaatgtggttaagcttgaaggtctagtcacttcaagaatgtcatgtagtctctacttggttttcgagtacatggagcatgatctctccggcctagccgcgtccacggggatcaagttcaccgagtctcaggtcaagtgctatatgaaacagttgctgtctggccttgaacattgccacaaccacggagtcttgcaccgtgatatcaaggggtctaatctactaattgacaatgaaggagttctgaaaatagctgattttggattggctacattttttgatcctcaacagaagcaacccttgactagtcgggttgttactctctggtaccgcccccctgagcttcttcttggggccacttgttacggagttggtgttgacctttggagtgctggctgcattttggcagagttgctttccgggaagcctattatgccaggacggacagaggttgaacaactgcataagatattcaagttatgtgggtccccatctgaggattactggaaaaaatacaaattgccaaatgcaacactttttaaaccacagcagccgtacaaacgctgcttggcagaaacgttcaaggacttcccaccttcttccctacctttaattgagtctcttctctcaatagactctgaaggacgaggcactgccactgctgctttaaatagtgaattctttactactgagccttttgcttgtgagccatcaagtttaccaaaatatcctcccagcaaggaaatggatgtaaagttacgagatgcagaatctagaaggcaaagaggtctaagtgggaagaccaatgctgatggtccaaaaaaagtcaaagctcgtgaccgagctggtcgagcaattccagttccagaagctaatgcagagaatcaaggaaacttagacaggtggagagctatgacacaaacaaacaccaagagcaagagtgagaaattcccacccccacatcaggatggagctgttggatacccccaagaagcatcacataaaggccctctatcatttgtcacaagtgatgcttcattcggatcagtggttttcaacacaaagtcttcaggatctgttaaaagcatgggagcggctggggggtcttcaaggcggaggaaaaacaacagagaaaaccctacgagggctccttcccggaaattcatccaggcgttcaagccctcctcgatagggctatcaatgaatctattatttagccatagtaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

533

Amino Acids

59.14

Weight (kDa)

9.59

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50860 AT5G50860
fragaria_vesca FvH4_5g09040
malus_domestica MD06G1134300.v1.1 MD14G1150700.v1.1
prunus_persica Prupe.5G145300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189181
rosa_laevigata RLG00000004626
rosa_multiflora Rmu_ssc0000251.1_g000001
rosa_roxburghii Rroxscaffold_3G00265730
rosa_rugosa Rorug06G0496600
rosa_samantha Rh7AG101200 Rh7BG103800 Rh7CG105200 Rh7DG103100
rosa_wichuraiana Rw7G008740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 699
AccB1I GGYRCC 1 cut(s) 699
AccBSI CCGCTC 2 cut(s) 100, 1466
AccI GTMKAC 1 cut(s) 12
AccII CGCG 3 cut(s) 167, 187, 473
AclI AACGTT 1 cut(s) 950
AclWI GGATC 5 cut(s) 163, 491, 647, 1423, 1453
AcoI YGGCCR 3 cut(s) 134, 150, 336
AcsI RAATTY 4 cut(s) 329, 1049, 1319, 1532
AcuI CTGAAG 2 cut(s) 1032, 1425
AcyI GRCGYC 1 cut(s) 182
AfaI GTAC 3 cut(s) 443, 701, 929
AfiI CCNNNNNNNGG 4 cut(s) 868, 1336, 1516, 1563
AhlI ACTAGT 1 cut(s) 677
AjnI CCWGG 3 cut(s) 171, 808, 1539
AluBI AGCT 9 cut(s) 211, 388, 626, 715, 1205, 1217, 1244, 1283, 1346
AluI AGCT 9 cut(s) 211, 388, 626, 715, 1205, 1217, 1244, 1283, 1346
Alw26I GTCTC 4 cut(s) 252, 428, 504, 996
AlwI GGATC 5 cut(s) 163, 491, 647, 1423, 1453
AlwNI CAGNNNCTG 2 cut(s) 529, 1448
AoxI GGCC 8 cut(s) 134, 150, 192, 336, 463, 535, 726, 1378
ApeKI GCWGC 5 cut(s) 355, 768, 922, 936, 1034
ApoI RAATTY 4 cut(s) 329, 1049, 1319, 1532
AseI ATTAAT 1 cut(s) 348
Asp700I GAANNNNTTC 1 cut(s) 612
Asp718I GGTACC 1 cut(s) 699
AspS9I GGNCC 5 cut(s) 192, 726, 858, 1187, 1379
AsuC2I CCSGG 2 cut(s) 793, 1528
AsuHPI GGTGA 2 cut(s) 130, 484
AvaII GGWCC 2 cut(s) 858, 1187
BalI TGGCCA 1 cut(s) 338
BanI GGYRCC 1 cut(s) 699
BanII GRGCYC 1 cut(s) 1522
BauI CACGAG 1 cut(s) 1206
BbsI GAAGAC 3 cut(s) 1178, 1431, 1470
BbvI GCAGC 5 cut(s) 342, 755, 923, 934, 1021
BccI CCATC 6 cut(s) 171, 378, 871, 1090, 1178, 1334
BceAI ACGGC 4 cut(s) 31, 121, 137, 910
BciT130I CCWGG 3 cut(s) 173, 810, 1541
BciVI GTATCC 1 cut(s) 1346
BcnI CCSGG 2 cut(s) 793, 1528
BcoDI GTCTC 4 cut(s) 252, 428, 504, 996
BcuI ACTAGT 1 cut(s) 677
BfaI CTAG 4 cut(s) 398, 467, 678, 1146
BfuI GTATCC 1 cut(s) 1346
BglI GCCNNNNNGGC 1 cut(s) 142
Bme1390I CCNGG 5 cut(s) 173, 793, 810, 1528, 1541
Bme18I GGWCC 2 cut(s) 858, 1187
BmgT120I GGNCC 5 cut(s) 192, 726, 858, 1187, 1379
BmiI GGNNCC 6 cut(s) 315, 701, 727, 859, 860, 1521
BmrFI CCNGG 5 cut(s) 173, 793, 810, 1528, 1541
BmsI GCATC 3 cut(s) 1126, 1376, 1393
BpiI GAAGAC 3 cut(s) 1178, 1431, 1470
Bpu10I CCTNAGC 1 cut(s) 711
BpuMI CCSGG 2 cut(s) 793, 1528
BsaHI GRCGYC 1 cut(s) 182
BsaJI CCNNGG 4 cut(s) 172, 185, 477, 556
BsaWI WCCGGW 1 cut(s) 88
BsaXI ACNNNNNCTCC 6 cut(s) 12, 42, 732, 762, 1504, 1534
Bsc4I CCNNNNNNNGG 4 cut(s) 868, 1336, 1516, 1563
Bse118I RCCGGY 2 cut(s) 77, 158
Bse1I ACTGG 2 cut(s) 881, 1232
Bse3DI GCAATG 2 cut(s) 247, 544
BseBI CCWGG 3 cut(s) 173, 810, 1541
BseDI CCNNGG 4 cut(s) 172, 185, 477, 556
BseGI GGATG 5 cut(s) 182, 370, 1126, 1345, 1536
BseLI CCNNNNNNNGG 4 cut(s) 868, 1336, 1516, 1563
BseMI GCAATG 2 cut(s) 247, 544
BseMII CTCAG 4 cut(s) 515, 702, 858, 1053
BseNI ACTGG 2 cut(s) 881, 1232
BseRI GAGGAG 1 cut(s) 1546
BseXI GCAGC 5 cut(s) 342, 755, 923, 934, 1021
BseYI CCCAGC 2 cut(s) 1106, 1469
Bsh1236I CGCG 3 cut(s) 167, 187, 473
BshFI GGCC 8 cut(s) 136, 152, 194, 338, 465, 537, 728, 1380
BshNI GGYRCC 1 cut(s) 699
BsiSI CCGG 7 cut(s) 78, 89, 159, 317, 462, 792, 1528
BslFI GGGAC 2 cut(s) 244, 844
BslI CCNNNNNNNGG 4 cut(s) 868, 1336, 1516, 1563
BsmAI GTCTC 4 cut(s) 252, 428, 504, 996
BsmFI GGGAC 2 cut(s) 244, 844
BsnI GGCC 8 cut(s) 136, 152, 194, 338, 465, 537, 728, 1380
Bsp1286I GDGCHC 1 cut(s) 1522
Bsp143I GATC 6 cut(s) 168, 454, 483, 652, 1415, 1445
BspANI GGCC 8 cut(s) 136, 152, 194, 338, 465, 537, 728, 1380
BspCNI CTCAG 4 cut(s) 514, 703, 859, 1054
BspFNI CGCG 3 cut(s) 167, 187, 473
BspLI GGNNCC 6 cut(s) 315, 701, 727, 859, 860, 1521
BspPI GGATC 5 cut(s) 163, 491, 647, 1423, 1453
BspT107I GGYRCC 1 cut(s) 699
BsrBI CCGCTC 2 cut(s) 100, 1466
BsrDI GCAATG 2 cut(s) 247, 544
BsrFI RCCGGY 2 cut(s) 77, 158
BsrI ACTGG 2 cut(s) 881, 1232
BssAI RCCGGY 2 cut(s) 77, 158
BssECI CCNNGG 4 cut(s) 172, 185, 477, 556
BssMI GATC 6 cut(s) 168, 454, 483, 652, 1415, 1445
BssNI GRCGYC 1 cut(s) 182
BssSI CACGAG 1 cut(s) 1206
Bst2BI CACGAG 1 cut(s) 1206
Bst2UI CCWGG 3 cut(s) 173, 810, 1541
Bst4CI ACNGT 2 cut(s) 525, 572
Bst6I CTCTTC 1 cut(s) 999
BstACI GRCGYC 1 cut(s) 182
BstC8I GCNNGC 4 cut(s) 134, 160, 192, 766
BstDEI CTNAG 6 cut(s) 501, 711, 867, 1062, 1163, 1267
BstDSI CCRYGG 3 cut(s) 185, 477, 556
BstF5I GGATG 5 cut(s) 182, 370, 1126, 1345, 1536
BstFNI CGCG 3 cut(s) 167, 187, 473
BstKTI GATC 6 cut(s) 171, 457, 486, 655, 1418, 1448
BstMAI GTCTC 4 cut(s) 252, 428, 504, 996
BstMBI GATC 6 cut(s) 168, 454, 483, 652, 1415, 1445
BstMWI GCNNNNNNNGC 7 cut(s) 106, 133, 142, 164, 805, 1034, 1223
BstNI CCWGG 3 cut(s) 173, 810, 1541
BstSCI CCNGG 5 cut(s) 171, 791, 808, 1526, 1539
BstUI CGCG 3 cut(s) 167, 187, 473
BstV1I GCAGC 5 cut(s) 342, 755, 923, 934, 1021
BstV2I GAAGAC 3 cut(s) 1178, 1431, 1470
BstX2I RGATCY 1 cut(s) 1445
BstYI RGATCY 1 cut(s) 1445
BsuI GTATCC 1 cut(s) 1346
BsuRI GGCC 8 cut(s) 136, 152, 194, 338, 465, 537, 728, 1380
BtgI CCRYGG 3 cut(s) 185, 477, 556
BtsCI GGATG 5 cut(s) 182, 370, 1126, 1345, 1536
BtsI GCAGTG 2 cut(s) 1023, 1029
BtsIMutI CAGTG 3 cut(s) 1023, 1029, 1425
Cac8I GCNNGC 4 cut(s) 134, 160, 192, 766
CaiI CAGNNNCTG 2 cut(s) 529, 1448
Cfr10I RCCGGY 2 cut(s) 77, 158
Cfr13I GGNCC 5 cut(s) 192, 726, 858, 1187, 1379
Cfr42I CCGCGG 1 cut(s) 188
CseI GACGC 3 cut(s) 173, 190, 462
Csp6I GTAC 3 cut(s) 442, 700, 928
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 5 cut(s) 334, 417, 445, 452, 1459
CviQI GTAC 3 cut(s) 442, 700, 928
DdeI CTNAG 6 cut(s) 501, 711, 867, 1062, 1163, 1267
DpnI GATC 6 cut(s) 170, 456, 485, 654, 1417, 1447
DpnII GATC 6 cut(s) 168, 454, 483, 652, 1415, 1445
DraI TTTAAA 2 cut(s) 913, 1041
EaeI YGGCCR 3 cut(s) 134, 150, 336
Eam1104I CTCTTC 1 cut(s) 999
EarI CTCTTC 1 cut(s) 999
EciI GGCGGA 3 cut(s) 33, 118, 1501
Eco24I GRGCYC 1 cut(s) 1522
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 858, 1187
Eco57I CTGAAG 2 cut(s) 1032, 1425
EcoO109I RGGNCCY 2 cut(s) 858, 1379
EcoRI GAATTC 1 cut(s) 1049
EcoRII CCWGG 3 cut(s) 171, 808, 1539
EcoRV GATATC 1 cut(s) 577
EcoT38I GRGCYC 1 cut(s) 1522
FaeI CATG 5 cut(s) 337, 420, 448, 455, 1462
FalI AAGNNNNNCTT 4 cut(s) 656, 688, 1390, 1422
FaqI GGGAC 2 cut(s) 244, 844
FatI CATG 5 cut(s) 333, 416, 444, 451, 1458
FauI CCCGC 1 cut(s) 183
FblI GTMKAC 1 cut(s) 12
FokI GGATG 5 cut(s) 189, 357, 1133, 1352, 1523
FriOI GRGCYC 1 cut(s) 1522
FspBI CTAG 4 cut(s) 398, 467, 678, 1146
GsaI CCCAGC 2 cut(s) 1110, 1473
HaeIII GGCC 8 cut(s) 136, 152, 194, 338, 465, 537, 728, 1380
HapII CCGG 7 cut(s) 78, 89, 159, 317, 462, 792, 1528
HgaI GACGC 3 cut(s) 173, 190, 462
Hin1I GRCGYC 1 cut(s) 182
Hin1II CATG 5 cut(s) 337, 420, 448, 455, 1462
HincII GTYRAC 2 cut(s) 13, 751
HindII GTYRAC 2 cut(s) 13, 751
HindIII AAGCTT 2 cut(s) 209, 386
HinfI GANTC 8 cut(s) 67, 497, 561, 989, 1007, 1142, 1255, 1576
HpaII CCGG 7 cut(s) 78, 89, 159, 317, 462, 792, 1528
HphI GGTGA 2 cut(s) 130, 484
Hpy166II GTNNAC 6 cut(s) 13, 121, 477, 492, 751, 1091
Hpy188I TCNGA 4 cut(s) 618, 868, 1012, 1415
Hpy188III TCNNGA 7 cut(s) 205, 408, 1146, 1208, 1238, 1337, 1443
Hpy8I GTNNAC 6 cut(s) 13, 121, 477, 492, 751, 1091
Hpy99I CGWCG 6 cut(s) 8, 14, 17, 142, 158, 167
HpyAV CCTTC 8 cut(s) 34, 286, 386, 602, 978, 1007, 1143, 1533
HpyCH4III ACNGT 2 cut(s) 525, 572
HpyCH4IV ACGT 1 cut(s) 950
HpyCH4V TGCA 6 cut(s) 568, 771, 835, 902, 1139, 1250
HpyF10VI GCNNNNNNNGC 7 cut(s) 106, 133, 142, 164, 805, 1034, 1223
HpyF3I CTNAG 6 cut(s) 501, 711, 867, 1062, 1163, 1267
HpySE526I ACGT 1 cut(s) 950
Hsp92I GRCGYC 1 cut(s) 182
Hsp92II CATG 5 cut(s) 337, 420, 448, 455, 1462
KflI GGGWCCC 1 cut(s) 858
KpnI GGTACC 1 cut(s) 703
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
KspI CCGCGG 1 cut(s) 188
Kzo9I GATC 6 cut(s) 168, 454, 483, 652, 1415, 1445
LmnI GCTCC 5 cut(s) 313, 448, 1343, 1463, 1525
Lsp1109I GCAGC 5 cut(s) 342, 755, 923, 934, 1021
LweI GCATC 3 cut(s) 1126, 1376, 1393
MaeI CTAG 4 cut(s) 398, 467, 678, 1146
MaeII ACGT 1 cut(s) 950
MaeIII GTNAC 6 cut(s) 400, 688, 734, 1128, 1208, 1393
MalI GATC 6 cut(s) 170, 456, 485, 654, 1417, 1447
MbiI CCGCTC 2 cut(s) 100, 1466
MboI GATC 6 cut(s) 168, 454, 483, 652, 1415, 1445
MboII GAAGA 6 cut(s) 710, 963, 986, 1183, 1431, 1470
MflI RGATCY 1 cut(s) 1445
MhlI GDGCHC 1 cut(s) 1522
MlsI TGGCCA 1 cut(s) 338
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 4 cut(s) 506, 570, 998, 1001
MmeI TCCRAC 1 cut(s) 1330
Mox20I TGGCCA 1 cut(s) 338
MreI CGCCGGCG 1 cut(s) 158
MroNI GCCGGC 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 612
MscI TGGCCA 1 cut(s) 338
MseI TTAA 8 cut(s) 263, 287, 348, 384, 912, 983, 1040, 1452
MslI CAYNNNNRTG 2 cut(s) 375, 1338
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 187
MspI CCGG 7 cut(s) 78, 89, 159, 317, 462, 792, 1528
MspR9I CCNGG 5 cut(s) 173, 793, 810, 1528, 1541
MvaI CCWGG 3 cut(s) 173, 810, 1541
MvnI CGCG 3 cut(s) 167, 187, 473
MwoI GCNNNNNNNGC 7 cut(s) 106, 133, 142, 164, 805, 1034, 1223
NaeI GCCGGC 1 cut(s) 160
NciI CCSGG 2 cut(s) 793, 1528
NdeII GATC 6 cut(s) 168, 454, 483, 652, 1415, 1445
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 5 cut(s) 337, 420, 448, 455, 1462
NlaIV GGNNCC 6 cut(s) 315, 701, 727, 859, 860, 1521
NmuCI GTSAC 3 cut(s) 400, 1208, 1393
PdiI GCCGGC 1 cut(s) 160
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 4 cut(s) 67, 1142, 1255, 1576
PfoI TCCNGGA 2 cut(s) 791, 1526
PleI GAGTC 4 cut(s) 505, 569, 997, 1001
PpsI GAGTC 4 cut(s) 505, 569, 997, 1001
PpuMI RGGWCCY 1 cut(s) 858
PshBI ATTAAT 1 cut(s) 348
Psp1406I AACGTT 1 cut(s) 950
Psp5II RGGWCCY 1 cut(s) 858
Psp6I CCWGG 3 cut(s) 171, 808, 1539
PspFI CCCAGC 2 cut(s) 1106, 1469
PspGI CCWGG 3 cut(s) 171, 808, 1539
PspN4I GGNNCC 6 cut(s) 315, 701, 727, 859, 860, 1521
PspPI GGNCC 5 cut(s) 192, 726, 858, 1187, 1379
PspPPI RGGWCCY 1 cut(s) 858
PstNI CAGNNNCTG 2 cut(s) 529, 1448
PsuI RGATCY 1 cut(s) 1445
RsaI GTAC 3 cut(s) 443, 701, 929
RsaNI GTAC 3 cut(s) 442, 700, 928
RseI CAYNNNNRTG 2 cut(s) 375, 1338
SacII CCGCGG 1 cut(s) 188
SalI GTCGAC 1 cut(s) 11
SaqAI TTAA 8 cut(s) 263, 287, 348, 384, 912, 983, 1040, 1452
Sau3AI GATC 6 cut(s) 168, 454, 483, 652, 1415, 1445
Sau96I GGNCC 5 cut(s) 192, 726, 858, 1187, 1379
SchI GAGTC 4 cut(s) 506, 570, 998, 1001
ScrFI CCNGG 5 cut(s) 173, 793, 810, 1528, 1541
SduI GDGCHC 1 cut(s) 1522
SfaNI GCATC 3 cut(s) 1126, 1376, 1393
Sfr303I CCGCGG 1 cut(s) 188
SgrAI CRCCGGYG 2 cut(s) 77, 158
SgrBI CCGCGG 1 cut(s) 188
SgrDI CGTCGACG 1 cut(s) 11
SinI GGWCC 2 cut(s) 858, 1187
SmiMI CAYNNNNRTG 2 cut(s) 375, 1338
SpeI ACTAGT 1 cut(s) 677
SspMI CTAG 4 cut(s) 398, 467, 678, 1146
StyD4I CCNGG 5 cut(s) 171, 791, 808, 1526, 1539
TaaI ACNGT 2 cut(s) 525, 572
TaiI ACGT 1 cut(s) 953
TaqI TCGA 5 cut(s) 12, 204, 438, 1222, 1559
TaqII GACCGA 1 cut(s) 1227
TatI WGTACW 1 cut(s) 441
TauI GCSGC 5 cut(s) 103, 187, 190, 473, 1469
TfiI GAWTC 4 cut(s) 67, 1142, 1255, 1576
Tru1I TTAA 8 cut(s) 263, 287, 348, 384, 912, 983, 1040, 1452
Tru9I TTAA 8 cut(s) 263, 287, 348, 384, 912, 983, 1040, 1452
TscAI CASTG 3 cut(s) 1030, 1036, 1425
TseFI GTSAC 3 cut(s) 400, 1208, 1393
TseI GCWGC 5 cut(s) 355, 768, 922, 936, 1034
Tsp45I GTSAC 3 cut(s) 400, 1208, 1393
TspDTI ATGAA 6 cut(s) 322, 533, 621, 1398, 1525, 1589
TspGWI ACGGA 3 cut(s) 573, 753, 829
TspRI CASTG 3 cut(s) 1030, 1036, 1425
VpaK11BI GGWCC 2 cut(s) 858, 1187
VspI ATTAAT 1 cut(s) 348
XapI RAATTY 4 cut(s) 329, 1049, 1319, 1532
XbaI TCTAGA 1 cut(s) 1145
XcmI CCANNNNNNNNNTGG 1 cut(s) 1114
XmiI GTMKAC 1 cut(s) 12
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 4 cut(s) 398, 467, 678, 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.