Rorug06G0496600

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014887
Physical Location & Seq
Forward (+)
61322321 .. 61323232
912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0496600.1

Sequence Viewer

Length: 912 bp
ATGTTTGAGGAATCTGTATCCTCCCTACCCTCAATTTGGGCCTCCATGAACAGCTGGTTAACCCCAACTGTTCTCTTCCTCCTCCTCCAGCTCATGATTGGCACCATCTTCATGGCTTCCAGCTTAGGCACCCAGAAGCAGCACGACCAAGACAACCACGACCAGCAACAGCAACAACAAGGCTTGGCGAGATCTCCCTCTGTTTTGCAGCGGTTCAAATCCATCAACTTCTACGCCTACAGATCTCCGGAACCGGCCACGAATTATGAAAAACCACCCGAATCCGAGATCCATTTCGCCTTCCCGCACGCCCAGGAGGTCGAACAGCCCAAACTCCCCAGATCTCCGTCCATGCTGGAGCGGCTCCAGTCCAAGTTCCAGCTCCACTTCCCGAAAGGGTCCAGCCCGCTACAGCCCCGAGATTTCGCAGCCCCGGAACCCCATTTCAGTACTTACGAGCACTCTCATGAAGACGAAGACGACGAAGAGTCGGAAGAGGAGGCTGCTGAAGAGTTGGATGAGGACCAATTCGAAGCCGAAGAAGAAGGTGAGGAGAAAGAGCAGACCCTGGACGAGATTTACAGCCAGCTAGATCTGCAGGACCACCACGTCAGCAGGACGACATCGGACACCCAGCCGGCTTCCGGCGACGTACCCGCGAAGCTGCCGAAGAAGATGAAGAAGTCGGCCAGCGCCAAGTCGGCATTTGCTCATTTCGAGGCGGCCGACATCGTGGAGAGCCGCCGGCCGGCGACCGTGAAGGAGAAGAAGGCAAAGGCGGCGGAGGACGACGCGGAGGTGGATTCGAAAGCCGATGACTTCATCAACAGGTTCAAGCAGCAGCTGAAGCTGCAGAGGCTGGACTCGATCATTAGGTACAAGGACATGGTCAACAGAGGAAGCGACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

303

Amino Acids

34.61

Weight (kDa)

4.9

Isoelectric Point (pI)

66.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 10 - 42 7.1e-12 Domain of unknown function (DUF4408)
DUF761 PF05553 265 - 299 2.2e-16 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50860 AT5G50860
fragaria_vesca FvH4_5g09040
malus_domestica MD06G1134300.v1.1 MD14G1150700.v1.1
prunus_persica Prupe.5G145300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189181
rosa_laevigata RLG00000004626
rosa_multiflora Rmu_ssc0000251.1_g000001
rosa_roxburghii Rroxscaffold_3G00265730
rosa_rugosa Rorug06G0496600
rosa_samantha Rh7AG101200 Rh7BG103800 Rh7CG105200 Rh7DG103100
rosa_wichuraiana Rw7G008740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 608
AccB1I GGYRCC 2 cut(s) 101, 128
AccBSI CCGCTC 1 cut(s) 361
AccII CGCG 2 cut(s) 659, 794
AccIII TCCGGA 1 cut(s) 247
AclWI GGATC 1 cut(s) 283
AcoI YGGCCR 4 cut(s) 255, 687, 723, 746
AcuI CTGAAG 2 cut(s) 528, 866
AfaI GTAC 3 cut(s) 451, 654, 878
AfiI CCNNNNNNNGG 3 cut(s) 36, 644, 748
AgsI TTSAA 2 cut(s) 217, 835
AhdI GACNNNNNGTC 1 cut(s) 487
AjiI CACGTC 1 cut(s) 610
AjnI CCWGG 2 cut(s) 312, 567
AluBI AGCT 8 cut(s) 54, 91, 123, 382, 589, 664, 844, 850
AluI AGCT 8 cut(s) 54, 91, 123, 382, 589, 664, 844, 850
Alw21I GWGCWC 1 cut(s) 462
AlwI GGATC 1 cut(s) 283
AlwNI CAGNNNCTG 3 cut(s) 568, 844, 859
Ama87I CYCGRG 1 cut(s) 417
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 5 cut(s) 39, 255, 687, 723, 746
ApeKI GCWGC 8 cut(s) 139, 208, 428, 503, 664, 838, 841, 850
AspLEI GCGC 1 cut(s) 695
AspS9I GGNCC 4 cut(s) 39, 399, 523, 601
AsuC2I CCSGG 1 cut(s) 434
AsuHPI GGTGA 1 cut(s) 560
AsuII TTCGAA 2 cut(s) 531, 806
AvaI CYCGRG 1 cut(s) 417
AvaII GGWCC 3 cut(s) 399, 523, 601
BanI GGYRCC 2 cut(s) 101, 128
BbsI GAAGAC 2 cut(s) 477, 483
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 8 cut(s) 151, 220, 440, 490, 651, 837, 850, 853
BccI CCATC 2 cut(s) 113, 230
BciT130I CCWGG 2 cut(s) 314, 569
BciVI GTATCC 1 cut(s) 28
BcnI CCSGG 1 cut(s) 434
BfaI CTAG 1 cut(s) 590
BfmI CTRYAG 4 cut(s) 238, 410, 596, 851
BfoI RGCGCY 1 cut(s) 696
BfuI GTATCC 1 cut(s) 28
BglI GCCNNNNNGGC 1 cut(s) 701
BglII AGATCT 4 cut(s) 191, 242, 341, 592
BmcAI AGTACT 1 cut(s) 451
Bme1390I CCNGG 3 cut(s) 314, 434, 569
Bme18I GGWCC 3 cut(s) 399, 523, 601
BmeRI GACNNNNNGTC 1 cut(s) 487
BmeT110I CYCGRG 1 cut(s) 417
BmgBI CACGTC 1 cut(s) 610
BmgT120I GGNCC 4 cut(s) 39, 399, 523, 601
BmiI GGNNCC 6 cut(s) 103, 130, 252, 365, 400, 438
BmrFI CCNGG 3 cut(s) 314, 434, 569
BpiI GAAGAC 2 cut(s) 477, 483
BpmI CTGGAG 3 cut(s) 71, 350, 377
Bpu10I CCTNAGC 1 cut(s) 124
Bpu14I TTCGAA 2 cut(s) 531, 806
BpuMI CCSGG 1 cut(s) 434
BsaJI CCNNGG 3 cut(s) 312, 432, 567
BsaWI WCCGGW 1 cut(s) 247
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 644, 748
Bse118I RCCGGY 4 cut(s) 253, 637, 744, 748
Bse1I ACTGG 1 cut(s) 367
BseAI TCCGGA 1 cut(s) 247
BseBI CCWGG 2 cut(s) 314, 569
BseDI CCNNGG 3 cut(s) 312, 432, 567
BseGI GGATG 1 cut(s) 523
BseLI CCNNNNNNNGG 3 cut(s) 36, 644, 748
BseNI ACTGG 1 cut(s) 367
BseRI GAGGAG 4 cut(s) 71, 74, 512, 566
BseX3I CGGCCG 2 cut(s) 723, 746
BseXI GCAGC 8 cut(s) 151, 220, 440, 490, 651, 837, 850, 853
BseYI CCCAGC 1 cut(s) 633
Bsh1236I CGCG 2 cut(s) 659, 794
Bsh1285I CGRYCG 3 cut(s) 726, 749, 756
BshFI GGCC 5 cut(s) 41, 257, 689, 725, 748
BshNI GGYRCC 2 cut(s) 101, 128
BsiEI CGRYCG 3 cut(s) 726, 749, 756
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 7 cut(s) 248, 254, 434, 638, 645, 745, 749
BslI CCNNNNNNNGG 3 cut(s) 36, 644, 748
BsnI GGCC 5 cut(s) 41, 257, 689, 725, 748
BsoBI CYCGRG 1 cut(s) 417
Bsp119I TTCGAA 2 cut(s) 531, 806
Bsp1286I GDGCHC 1 cut(s) 462
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 6 cut(s) 191, 242, 288, 341, 592, 867
BspANI GGCC 5 cut(s) 41, 257, 689, 725, 748
BspEI TCCGGA 1 cut(s) 247
BspFNI CGCG 2 cut(s) 659, 794
BspHI TCATGA 2 cut(s) 93, 466
BspLI GGNNCC 6 cut(s) 103, 130, 252, 365, 400, 438
BspMAI CTGCAG 2 cut(s) 600, 855
BspPI GGATC 1 cut(s) 283
BspT104I TTCGAA 2 cut(s) 531, 806
BspT107I GGYRCC 2 cut(s) 101, 128
BsrBI CCGCTC 1 cut(s) 361
BsrFI RCCGGY 4 cut(s) 253, 637, 744, 748
BsrI ACTGG 1 cut(s) 367
BssAI RCCGGY 4 cut(s) 253, 637, 744, 748
BssECI CCNNGG 3 cut(s) 312, 432, 567
BssMI GATC 6 cut(s) 191, 242, 288, 341, 592, 867
Bst2UI CCWGG 2 cut(s) 314, 569
Bst4CI ACNGT 2 cut(s) 70, 757
Bst6I CTCTTC 4 cut(s) 80, 480, 489, 504
BstBI TTCGAA 2 cut(s) 531, 806
BstC8I GCNNGC 7 cut(s) 309, 407, 587, 639, 691, 746, 750
BstDEI CTNAG 1 cut(s) 124
BstF5I GGATG 1 cut(s) 523
BstFNI CGCG 2 cut(s) 659, 794
BstH2I RGCGCY 1 cut(s) 696
BstHHI GCGC 1 cut(s) 695
BstKTI GATC 6 cut(s) 194, 245, 291, 344, 595, 870
BstMBI GATC 6 cut(s) 191, 242, 288, 341, 592, 867
BstMCI CGRYCG 3 cut(s) 726, 749, 756
BstMWI GCNNNNNNNGC 7 cut(s) 361, 595, 701, 779, 847, 850, 856
BstNI CCWGG 2 cut(s) 314, 569
BstSCI CCNGG 3 cut(s) 312, 432, 567
BstSFI CTRYAG 4 cut(s) 238, 410, 596, 851
BstUI CGCG 2 cut(s) 659, 794
BstV1I GCAGC 8 cut(s) 151, 220, 440, 490, 651, 837, 850, 853
BstV2I GAAGAC 2 cut(s) 477, 483
BstX2I RGATCY 5 cut(s) 191, 242, 288, 341, 592
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 5 cut(s) 191, 242, 288, 341, 592
BstZI CGGCCG 2 cut(s) 723, 746
BsuI GTATCC 1 cut(s) 28
BsuRI GGCC 5 cut(s) 41, 257, 689, 725, 748
BtrI CACGTC 1 cut(s) 610
BtsCI GGATG 1 cut(s) 523
Cac8I GCNNGC 7 cut(s) 309, 407, 587, 639, 691, 746, 750
CaiI CAGNNNCTG 3 cut(s) 568, 844, 859
CciI TCATGA 2 cut(s) 93, 466
CfoI GCGC 1 cut(s) 695
Cfr10I RCCGGY 4 cut(s) 253, 637, 744, 748
Cfr13I GGNCC 4 cut(s) 39, 399, 523, 601
CseI GACGC 1 cut(s) 800
Csp6I GTAC 3 cut(s) 450, 653, 877
CviAII CATG 6 cut(s) 46, 94, 112, 352, 467, 886
CviQI GTAC 3 cut(s) 450, 653, 877
DdeI CTNAG 1 cut(s) 124
DpnI GATC 6 cut(s) 193, 244, 290, 343, 594, 869
DpnII GATC 6 cut(s) 191, 242, 288, 341, 592, 867
DrdI GACNNNNNNGTC 1 cut(s) 608
DriI GACNNNNNGTC 1 cut(s) 487
DseDI GACNNNNNNGTC 1 cut(s) 608
EaeI YGGCCR 4 cut(s) 255, 687, 723, 746
EagI CGGCCG 2 cut(s) 723, 746
Eam1104I CTCTTC 4 cut(s) 80, 480, 489, 504
Eam1105I GACNNNNNGTC 1 cut(s) 487
EarI CTCTTC 4 cut(s) 80, 480, 489, 504
EciI GGCGGA 1 cut(s) 797
EclXI CGGCCG 2 cut(s) 723, 746
Eco47I GGWCC 3 cut(s) 399, 523, 601
Eco52I CGGCCG 2 cut(s) 723, 746
Eco57I CTGAAG 2 cut(s) 528, 866
Eco88I CYCGRG 1 cut(s) 417
EcoRII CCWGG 2 cut(s) 312, 567
FaeI CATG 6 cut(s) 49, 97, 115, 355, 470, 889
FaiI YATR 7 cut(s) 47, 95, 113, 267, 353, 468, 887
FatI CATG 6 cut(s) 45, 93, 111, 351, 466, 885
FauI CCCGC 3 cut(s) 312, 414, 664
FokI GGATG 1 cut(s) 530
FspBI CTAG 1 cut(s) 590
GlaI GCGC 1 cut(s) 694
GsaI CCCAGC 1 cut(s) 637
GsuI CTGGAG 3 cut(s) 71, 350, 377
HaeII RGCGCY 1 cut(s) 696
HaeIII GGCC 5 cut(s) 41, 257, 689, 725, 748
HapII CCGG 7 cut(s) 248, 254, 434, 638, 645, 745, 749
HgaI GACGC 1 cut(s) 800
HhaI GCGC 1 cut(s) 695
Hin1II CATG 6 cut(s) 49, 97, 115, 355, 470, 889
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HincII GTYRAC 2 cut(s) 60, 892
HindII GTYRAC 2 cut(s) 60, 892
HinfI GANTC 5 cut(s) 11, 281, 488, 803, 863
HpaI GTTAAC 1 cut(s) 60
HpaII CCGG 7 cut(s) 248, 254, 434, 638, 645, 745, 749
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 2 cut(s) 60, 892
Hpy188I TCNGA 3 cut(s) 286, 493, 628
Hpy188III TCNNGA 4 cut(s) 94, 248, 391, 467
Hpy8I GTNNAC 2 cut(s) 60, 892
Hpy99I CGWCG 3 cut(s) 485, 653, 794
HpyAV CCTTC 4 cut(s) 310, 539, 754, 763
HpyCH4III ACNGT 2 cut(s) 70, 757
HpyCH4IV ACGT 2 cut(s) 609, 651
HpyCH4V TGCA 3 cut(s) 208, 598, 853
HpyF10VI GCNNNNNNNGC 7 cut(s) 361, 595, 701, 779, 847, 850, 856
HpyF3I CTNAG 1 cut(s) 124
HpySE526I ACGT 2 cut(s) 609, 651
Hsp92II CATG 6 cut(s) 49, 97, 115, 355, 470, 889
HspAI GCGC 1 cut(s) 693
Kpn2I TCCGGA 1 cut(s) 247
KroI GCCGGC 3 cut(s) 637, 744, 748
KroNI GCCGGC 3 cut(s) 639, 746, 750
KspAI GTTAAC 1 cut(s) 60
Kzo9I GATC 6 cut(s) 191, 242, 288, 341, 592, 867
LmnI GCTCC 3 cut(s) 358, 369, 387
Lsp1109I GCAGC 8 cut(s) 151, 220, 440, 490, 651, 837, 850, 853
MaeI CTAG 1 cut(s) 590
MaeII ACGT 2 cut(s) 609, 651
MalI GATC 6 cut(s) 193, 244, 290, 343, 594, 869
MbiI CCGCTC 1 cut(s) 361
MboI GATC 6 cut(s) 191, 242, 288, 341, 592, 867
MflI RGATCY 5 cut(s) 191, 242, 288, 341, 592
MhlI GDGCHC 1 cut(s) 462
MluCI AATT 3 cut(s) 33, 262, 527
MlyI GAGTC 2 cut(s) 497, 857
MmeI TCCRAC 2 cut(s) 471, 495
MroI TCCGGA 1 cut(s) 247
MroNI GCCGGC 3 cut(s) 637, 744, 748
MseI TTAA 1 cut(s) 59
MslI CAYNNNNRTG 2 cut(s) 110, 465
MspA1I CMGCKG 3 cut(s) 54, 211, 844
MspI CCGG 7 cut(s) 248, 254, 434, 638, 645, 745, 749
MspR9I CCNGG 3 cut(s) 314, 434, 569
MvaI CCWGG 2 cut(s) 314, 569
MvnI CGCG 2 cut(s) 659, 794
MwoI GCNNNNNNNGC 7 cut(s) 361, 595, 701, 779, 847, 850, 856
NaeI GCCGGC 3 cut(s) 639, 746, 750
NciI CCSGG 1 cut(s) 434
NdeII GATC 6 cut(s) 191, 242, 288, 341, 592, 867
NgoMIV GCCGGC 3 cut(s) 637, 744, 748
NlaIII CATG 6 cut(s) 49, 97, 115, 355, 470, 889
NlaIV GGNNCC 6 cut(s) 103, 130, 252, 365, 400, 438
NspV TTCGAA 2 cut(s) 531, 806
PagI TCATGA 2 cut(s) 93, 466
PcsI WCGNNNNNNNCGW 2 cut(s) 480, 723
PdiI GCCGGC 3 cut(s) 639, 746, 750
PfeI GAWTC 3 cut(s) 11, 281, 803
PflFI GACNNNGTC 1 cut(s) 887
PleI GAGTC 2 cut(s) 496, 857
PpsI GAGTC 2 cut(s) 496, 857
Psp6I CCWGG 2 cut(s) 312, 567
PspFI CCCAGC 1 cut(s) 633
PspGI CCWGG 2 cut(s) 312, 567
PspN4I GGNNCC 6 cut(s) 103, 130, 252, 365, 400, 438
PspPI GGNCC 4 cut(s) 39, 399, 523, 601
PstI CTGCAG 2 cut(s) 600, 855
PstNI CAGNNNCTG 3 cut(s) 568, 844, 859
PsuI RGATCY 5 cut(s) 191, 242, 288, 341, 592
PsyI GACNNNGTC 1 cut(s) 887
PvuII CAGCTG 2 cut(s) 54, 844
RsaI GTAC 3 cut(s) 451, 654, 878
RsaNI GTAC 3 cut(s) 450, 653, 877
RseI CAYNNNNRTG 2 cut(s) 110, 465
SaqAI TTAA 1 cut(s) 59
Sau3AI GATC 6 cut(s) 191, 242, 288, 341, 592, 867
Sau96I GGNCC 4 cut(s) 39, 399, 523, 601
ScaI AGTACT 1 cut(s) 451
SchI GAGTC 2 cut(s) 497, 857
ScrFI CCNGG 3 cut(s) 314, 434, 569
SduI GDGCHC 1 cut(s) 462
SfcI CTRYAG 4 cut(s) 238, 410, 596, 851
SfuI TTCGAA 2 cut(s) 531, 806
SinI GGWCC 3 cut(s) 399, 523, 601
SmiMI CAYNNNNRTG 2 cut(s) 110, 465
Sse9I AATT 3 cut(s) 33, 262, 527
SspMI CTAG 1 cut(s) 590
StyD4I CCNGG 3 cut(s) 312, 432, 567
TaaI ACNGT 2 cut(s) 70, 757
TaiI ACGT 2 cut(s) 612, 654
TaqI TCGA 5 cut(s) 321, 531, 717, 806, 866
TasI AATT 3 cut(s) 33, 262, 527
TatI WGTACW 1 cut(s) 449
TauI GCSGC 4 cut(s) 364, 725, 744, 782
TfiI GAWTC 3 cut(s) 11, 281, 803
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TseI GCWGC 8 cut(s) 139, 208, 428, 503, 664, 838, 841, 850
TspDTI ATGAA 6 cut(s) 62, 100, 282, 483, 692, 811
TspGWI ACGGA 1 cut(s) 336
Tth111I GACNNNGTC 1 cut(s) 887
VpaK11BI GGWCC 3 cut(s) 399, 523, 601
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XspI CTAG 1 cut(s) 590
ZrmI AGTACT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.