AT5G50890

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012134
Physical Location & Seq
Forward (+)
20702453 .. 20704418
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50890.1

Sequence Viewer

Length: 1227 bp
ATGGCGGCGAATCGTGTGGCGATGGAAGAAGATATTACTGAATCTGTGACTTTAATGGTGGATTCCGTTTCTTCTTCTCCGATGACTCCGAAGACTGTGACGGTTAAGGAAGATGAAACTCATCATCCTTATGCGTTTCATGTTTCCGGTCCGAGAAATGTTGCTTCTCCTAATTGGAGAGATCTCATCAACTCCAGTTGGAAGGATCCTAACTACAAGAGAACAGTAATGGCCTGTTTTATTCAAGCAGCTTACTTGCTTGAACTCGACAGACAAGAGAATCGAAACGAGCAAAACGCTCTCGCACCAAAATGGTGGATTCCATTCAAGTACAAGTTATCACAAACGCTCATTGACGAGAGAGATGGATCAATATTTGGTGCTGTTCTTGAATGGGACAGAGCAGCTGCAATGGCAGATTTGGTTGTCATCAGACCAAGTGGGGCACCAAAAGCAGTACTTGCGCTGCGAGGAACGATACTAAAGAGTCTTACAATGAGACGTGACATAGAAGATGATCTCCGATTTTTAGCTTGGGAGAGCCTTAAAGGTTCTGTGAGGTTTAATGTTGCTCTAGAGGCGTTGCAATCAGTAGCAACGAGATATGGGAGCAGTAATGTCTGTGTAGTGGGGCATTCGTTAGGAGCTGGTTTTGCTTTACAAGTTGGTAAATCCTTGGCTAAAGAAGGGTTATTTGTAGATGCTCATTTGTTCAATCCACCTTCTATATCTGTCGCTATGAGCTTGAGAAACATCAGTGAGAAAGCTGGTGTTGCTTGGAAAAGACTCATGTCAATGCTTCCTCCCAAACACGAGCCTCTGATACAGAATAGTAACGAAGGTCAGGTTTCTCCTAGCAATGTTTCATCAGGGTTCAGGAACTGGGTACCGAGTTTCTACGGGTCGAATCAGAAACCTACTGTGGATTTAAGAAAATGGGTGCCTCATCTATATGTGAACGATAGCGACTATATTTGTTGCCACTACACTGACCAGGATGGAGTAGCAGAGAAGAGGGAAATGAACAACAAAGAGAACAATAGCCCTGTTGTAAACACTAATTTTCAAGCAGCAGCAAAGCTTTTCGTGATGTCGAAAGGGAAACAGAAGTTTCACGAGGCGCACGGGTTAGAACAATGGTGGTCTGATAACCTAGAGCTTCAATCAGCTATTCACAGCAGCAGGTTGATCAGTCAGCAACTCAAGTCATTATACAGTATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

408

Amino Acids

45.77

Weight (kDa)

8.51

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50890
fragaria_vesca FvH4_6g38560
malus_domestica MD09G1149800.v1.1 MD17G1136700.v1.1
prunus_persica Prupe.3G176300_v2.0.a1
pyrus_communis pycom09g06920 pycom17g13030
rosa_chinensis RchiOBHm_Chr2g0151971
rosa_laevigata RLG00000020595
rosa_multiflora Rmu_sc0005294.1_g000001 Rmu_sc0023925.1_g000001
rosa_roxburghii Rroxscaffold_2G00096090
rosa_rugosa Rorug02G0430000
rosa_samantha Rh2AG490600 Rh2BG502900 Rh2CG477000 Rh2DG514100
rosa_wichuraiana Rw0G020220 Rw2G040320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1173
Acc65I GGTACC 1 cut(s) 886
AccB1I GGYRCC 3 cut(s) 445, 886, 940
AciI CCGC 1 cut(s) 5
AclWI GGATC 3 cut(s) 200, 213, 376
AfaI GTAC 3 cut(s) 332, 459, 888
AgsI TTSAA 7 cut(s) 245, 263, 328, 392, 715, 1067, 1163
AjiI CACGTC 1 cut(s) 503
AjnI CCWGG 1 cut(s) 993
AluBI AGCT 9 cut(s) 251, 407, 533, 647, 744, 767, 1081, 1159, 1169
AluI AGCT 9 cut(s) 251, 407, 533, 647, 744, 767, 1081, 1159, 1169
Alw26I GTCTC 1 cut(s) 493
AlwI GGATC 3 cut(s) 200, 213, 376
AlwNI CAGNNNCTG 1 cut(s) 882
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 7 cut(s) 248, 404, 407, 466, 1070, 1073, 1179
Asp718I GGTACC 1 cut(s) 886
AspLEI GCGC 2 cut(s) 466, 1123
AspS9I GGNCC 1 cut(s) 149
AvaII GGWCC 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 448
BamHI GGATCC 1 cut(s) 205
BanI GGYRCC 3 cut(s) 445, 886, 940
BauI CACGAG 2 cut(s) 812, 1115
BbsI GAAGAC 1 cut(s) 98
BbvI GCAGC 7 cut(s) 260, 394, 416, 453, 1082, 1085, 1191
BccI CCATC 3 cut(s) 16, 359, 992
BciT130I CCWGG 1 cut(s) 995
BclI TGATCA 1 cut(s) 1188
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 3 cut(s) 575, 855, 1154
BfuAI ACCTGC 1 cut(s) 1173
BglII AGATCT 1 cut(s) 181
BisI GCNGC 8 cut(s) 6, 249, 405, 408, 467, 1071, 1074, 1180
BlsI GCNGC 8 cut(s) 7, 250, 406, 409, 468, 1072, 1075, 1181
BmcAI AGTACT 1 cut(s) 459
Bme1390I CCNGG 1 cut(s) 995
Bme18I GGWCC 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 503
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 4 cut(s) 207, 447, 888, 942
BmrFI CCNGG 1 cut(s) 995
BmrI ACTGGG 1 cut(s) 892
BmsI GCATC 1 cut(s) 691
BmuI ACTGGG 1 cut(s) 892
BoxI GACNNNNGTC 1 cut(s) 790
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 178
BpuEI CTTGAG 2 cut(s) 766, 1187
BsaJI CCNNGG 1 cut(s) 675
BsaWI WCCGGW 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 195, 887
Bse3DI GCAATG 2 cut(s) 417, 865
BseBI CCWGG 1 cut(s) 995
BseDI CCNNGG 1 cut(s) 675
BseGI GGATG 2 cut(s) 124, 1003
BseMI GCAATG 2 cut(s) 417, 865
BseNI ACTGG 2 cut(s) 195, 887
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 7 cut(s) 260, 394, 416, 453, 1082, 1085, 1191
BshFI GGCC 1 cut(s) 233
BshNI GGYRCC 3 cut(s) 445, 886, 940
BsiSI CCGG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 410
BsmAI GTCTC 1 cut(s) 493
BsmBI CGTCTC 1 cut(s) 493
BsmFI GGGAC 1 cut(s) 410
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 448
Bsp143I GATC 5 cut(s) 181, 205, 368, 517, 1188
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 233
BspLI GGNNCC 4 cut(s) 207, 447, 888, 942
BspMI ACCTGC 1 cut(s) 1173
BspPI GGATC 3 cut(s) 200, 213, 376
BspT107I GGYRCC 3 cut(s) 445, 886, 940
BsrDI GCAATG 2 cut(s) 417, 865
BsrI ACTGG 2 cut(s) 195, 887
BssECI CCNNGG 1 cut(s) 675
BssMI GATC 5 cut(s) 181, 205, 368, 517, 1188
BssSI CACGAG 2 cut(s) 812, 1115
BssT1I CCWWGG 1 cut(s) 675
Bst2BI CACGAG 2 cut(s) 812, 1115
Bst2UI CCWGG 1 cut(s) 995
Bst4CI ACNGT 5 cut(s) 97, 103, 226, 922, 1217
Bst6I CTCTTC 1 cut(s) 1007
BstAPI GCANNNNNTGC 1 cut(s) 461
BstF5I GGATG 2 cut(s) 124, 1003
BstHHI GCGC 2 cut(s) 466, 1123
BstKTI GATC 5 cut(s) 184, 208, 371, 520, 1191
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 5 cut(s) 181, 205, 368, 517, 1188
BstMWI GCNNNNNNNGC 6 cut(s) 413, 452, 461, 578, 653, 773
BstNI CCWGG 1 cut(s) 995
BstPAI GACNNNNGTC 1 cut(s) 790
BstSCI CCNGG 1 cut(s) 993
BstSLI GKGCMC 1 cut(s) 448
BstV1I GCAGC 7 cut(s) 260, 394, 416, 453, 1082, 1085, 1191
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 2 cut(s) 181, 205
BstXI CCANNNNNNTGG 1 cut(s) 315
BstYI RGATCY 2 cut(s) 181, 205
BsuRI GGCC 1 cut(s) 233
BtgZI GCGATG 1 cut(s) 35
BtrI CACGTC 1 cut(s) 503
BtsCI GGATG 2 cut(s) 124, 1003
BtsIMutI CAGTG 2 cut(s) 763, 987
BveI ACCTGC 1 cut(s) 1173
CaiI CAGNNNCTG 1 cut(s) 882
CfoI GCGC 2 cut(s) 466, 1123
Cfr13I GGNCC 1 cut(s) 149
CpoI CGGWCCG 1 cut(s) 149
Csp6I GTAC 3 cut(s) 331, 458, 887
CspI CGGWCCG 1 cut(s) 149
CviAII CATG 2 cut(s) 140, 790
CviQI GTAC 3 cut(s) 331, 458, 887
DpnI GATC 5 cut(s) 183, 207, 370, 519, 1190
DpnII GATC 5 cut(s) 181, 205, 368, 517, 1188
Eam1104I CTCTTC 1 cut(s) 1007
EarI CTCTTC 1 cut(s) 1007
Eco130I CCWWGG 1 cut(s) 675
Eco47I GGWCC 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 993
EcoT14I CCWWGG 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 675
Esp3I CGTCTC 1 cut(s) 493
FaeI CATG 2 cut(s) 143, 793
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FaqI GGGAC 1 cut(s) 410
FatI CATG 2 cut(s) 139, 789
FbaI TGATCA 1 cut(s) 1188
Fnu4HI GCNGC 8 cut(s) 6, 249, 405, 408, 467, 1071, 1074, 1180
FokI GGATG 2 cut(s) 111, 1010
Fsp4HI GCNGC 8 cut(s) 6, 249, 405, 408, 467, 1071, 1074, 1180
FspBI CTAG 3 cut(s) 575, 855, 1154
GlaI GCGC 2 cut(s) 465, 1122
GluI GCNGC 8 cut(s) 6, 249, 405, 408, 467, 1071, 1074, 1180
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 1 cut(s) 233
HapII CCGG 1 cut(s) 147
HhaI GCGC 2 cut(s) 466, 1123
Hin1II CATG 2 cut(s) 143, 793
Hin6I GCGC 2 cut(s) 464, 1121
HinP1I GCGC 2 cut(s) 464, 1121
HindIII AAGCTT 1 cut(s) 1079
HinfI GANTC 9 cut(s) 10, 41, 62, 85, 280, 319, 487, 786, 907
HpaII CCGG 1 cut(s) 147
Hpy166II GTNNAC 2 cut(s) 958, 1054
Hpy188I TCNGA 8 cut(s) 81, 90, 153, 434, 524, 822, 912, 1147
Hpy188III TCNNGA 5 cut(s) 389, 575, 877, 1087, 1115
Hpy8I GTNNAC 2 cut(s) 958, 1054
HpyAV CCTTC 4 cut(s) 196, 680, 732, 833
HpyCH4III ACNGT 5 cut(s) 97, 103, 226, 922, 1217
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 2 cut(s) 410, 586
HpyF10VI GCNNNNNNNGC 6 cut(s) 413, 452, 461, 578, 653, 773
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 2 cut(s) 143, 793
HspAI GCGC 2 cut(s) 464, 1121
KpnI GGTACC 1 cut(s) 890
Ksp22I TGATCA 1 cut(s) 1188
Kzo9I GATC 5 cut(s) 181, 205, 368, 517, 1188
LmnI GCTCC 2 cut(s) 609, 644
Lsp1109I GCAGC 7 cut(s) 260, 394, 416, 453, 1082, 1085, 1191
LweI GCATC 1 cut(s) 691
MaeI CTAG 3 cut(s) 575, 855, 1154
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 4 cut(s) 46, 97, 503, 833
MalI GATC 5 cut(s) 183, 207, 370, 519, 1190
MboI GATC 5 cut(s) 181, 205, 368, 517, 1188
MboII GAAGA 8 cut(s) 38, 41, 63, 66, 103, 122, 524, 1024
MflI RGATCY 2 cut(s) 181, 205
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 2 cut(s) 172, 1060
MlyI GAGTC 3 cut(s) 79, 496, 780
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 8 cut(s) 464, 552, 571, 813, 828, 954, 1008, 1111
MseI TTAA 5 cut(s) 53, 105, 546, 564, 929
MslI CAYNNNNRTG 4 cut(s) 129, 310, 794, 951
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 995
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 1 cut(s) 995
MwoI GCNNNNNNNGC 6 cut(s) 413, 452, 461, 578, 653, 773
NdeII GATC 5 cut(s) 181, 205, 368, 517, 1188
NlaIII CATG 2 cut(s) 143, 793
NlaIV GGNNCC 4 cut(s) 207, 447, 888, 942
NmuCI GTSAC 3 cut(s) 46, 97, 503
PctI GAATGC 1 cut(s) 634
PfeI GAWTC 6 cut(s) 10, 41, 62, 280, 319, 907
PkrI GCNGC 8 cut(s) 7, 250, 406, 409, 468, 1072, 1075, 1181
PleI GAGTC 3 cut(s) 79, 495, 780
PpsI GAGTC 3 cut(s) 79, 495, 780
PshAI GACNNNNGTC 1 cut(s) 790
Psp6I CCWGG 1 cut(s) 993
PspGI CCWGG 1 cut(s) 993
PspN4I GGNNCC 4 cut(s) 207, 447, 888, 942
PspPI GGNCC 1 cut(s) 149
PstNI CAGNNNCTG 1 cut(s) 882
PsuI RGATCY 2 cut(s) 181, 205
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 3 cut(s) 332, 459, 888
RsaNI GTAC 3 cut(s) 331, 458, 887
RseI CAYNNNNRTG 4 cut(s) 129, 310, 794, 951
Rsr2I CGGWCCG 1 cut(s) 149
RsrII CGGWCCG 1 cut(s) 149
SaqAI TTAA 5 cut(s) 53, 105, 546, 564, 929
SatI GCNGC 8 cut(s) 6, 249, 405, 408, 467, 1071, 1074, 1180
Sau3AI GATC 5 cut(s) 181, 205, 368, 517, 1188
Sau96I GGNCC 1 cut(s) 149
ScaI AGTACT 1 cut(s) 459
SchI GAGTC 3 cut(s) 79, 496, 780
ScrFI CCNGG 1 cut(s) 995
SduI GDGCHC 1 cut(s) 448
SfaNI GCATC 1 cut(s) 691
SinI GGWCC 1 cut(s) 149
SmiMI CAYNNNNRTG 4 cut(s) 129, 310, 794, 951
SmlI CTYRAG 2 cut(s) 745, 1202
SmoI CTYRAG 2 cut(s) 745, 1202
Sse9I AATT 2 cut(s) 172, 1060
SsiI CCGC 1 cut(s) 5
SspI AATATT 1 cut(s) 375
SspMI CTAG 3 cut(s) 575, 855, 1154
StyD4I CCNGG 1 cut(s) 993
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 5 cut(s) 97, 103, 226, 922, 1217
TaiI ACGT 1 cut(s) 505
TaqI TCGA 4 cut(s) 267, 283, 905, 1094
TasI AATT 2 cut(s) 172, 1060
TatI WGTACW 2 cut(s) 330, 457
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 6 cut(s) 10, 41, 62, 280, 319, 907
Tru1I TTAA 5 cut(s) 53, 105, 546, 564, 929
Tru9I TTAA 5 cut(s) 53, 105, 546, 564, 929
TscAI CASTG 2 cut(s) 763, 994
TseFI GTSAC 3 cut(s) 46, 97, 503
TseI GCWGC 7 cut(s) 248, 404, 407, 466, 1070, 1073, 1179
Tsp45I GTSAC 3 cut(s) 46, 97, 503
TspDTI ATGAA 4 cut(s) 128, 129, 855, 1037
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 2 cut(s) 763, 994
VpaK11BI GGWCC 1 cut(s) 149
XbaI TCTAGA 1 cut(s) 574
XspI CTAG 3 cut(s) 575, 855, 1154
ZrmI AGTACT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.