FvH4_6g38560

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012134
Physical Location & Seq
Reverse (-)
30521104 .. 30523599
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38560.t1

Sequence Viewer

Length: 1185 bp
ATGCAAGCAACCCCAAAACAAAGCCAAGCAGAAGAAGAGAGCGGCTGTGCCGCCGCCGTGTTAATGGAGAAAGTTGCGGAATTGCAGCCTCATCCCTACTCATTTCATGTTTCGGGTCCTCGGAAACTGTGCTCGCCCAATTGGAGAGACAATATAAGCTCAAGTTGGAAGGATGCGAATTACAAAAGAACTGTCATCGCATGCTTCATACAAGCAATCTACTTGCTTGAACTTGATAGACAGGAGAACAGAGATGCCGAAAATGCTCTTGCCCCCAAGTGGTGGATACCTTTTAAGTATAAACTAACCAAAACTTTAATTGATGAAAGAGATGGATCCATTTTCGGTGCAATTCTAGAATGGGACCGGTCTGCAGCATTAGCTGATTTTGTAGTCATGAGACCGAGTGGGGCACCAAAAGCTGTTTTAGCACTTAGGGGAACATTACTCAAAGGCCCAACTATGCGAAGGGATATAGAAGATGACCTTCGTTTTCTTGCTTGGGAGAGCCTGAAGGGATCTGTCAGGTTCAAAGTAGCTCTGGAGGCCTTGAAATCAGTTGCAGAAAGGTATGGAAGCAGTAATGTATGCATCGCAGGCCATTCTCTAGGAGCTGGTTTTGCTCTACAAGTGGGAAAAGCATTAGCTAAAGAAGGGATGTATGTAGAGACTCACTTATTCAATCCGCCTTCGGTTTCTCTAGCAATGAGCTTCAGGAATATTGGAGAGAAAGCAGGATTTGCATGGAAGAGATTTAAATCAATGCTTCCTATGAGTAGTGAAACTCAGGTCGACAATGAAGATGACAAGGGTATTGGTTTTGGATTGAAGAATTGGATATCGGGTTTAAGAGGTCCTCCTGGTGTTGGATTGGGGAAGTGGGTTCCTCATCTATATGTAAATAATAGTGACTATATCTGCTGCTCTTATACTGACCCTGATGGAATAGAAGCAAATAATGCTGGGAAGGAGAATGTGGGTCCAAGTTCAACAACAGGACAAGTAGCAGCAAAGCTATTTGTGATGTCAAAGGGAAACCAGAAGTTTCTTGAAGCTCATGGATTAGAGCAATGGTGGTCTGATGATCTGGAACTTCAGATGGCTCTCCATAACAGTAAACTCATCAGTAGGCAGCTGAAATCCTTGTATAGCCTTCCAGCTCCTCAGCCAGCACTTCCTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

395

Amino Acids

43.6

Weight (kDa)

8.58

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50890
fragaria_vesca FvH4_6g38560
malus_domestica MD09G1149800.v1.1 MD17G1136700.v1.1
prunus_persica Prupe.3G176300_v2.0.a1
pyrus_communis pycom09g06920 pycom17g13030
rosa_chinensis RchiOBHm_Chr2g0151971
rosa_laevigata RLG00000020595
rosa_multiflora Rmu_sc0005294.1_g000001 Rmu_sc0023925.1_g000001
rosa_roxburghii Rroxscaffold_2G00096090
rosa_rugosa Rorug02G0430000
rosa_samantha Rh2AG490600 Rh2BG502900 Rh2CG477000 Rh2DG514100
rosa_wichuraiana Rw0G020220 Rw2G040320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 412
AccB7I CCANNNNNTGG 1 cut(s) 282
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 792
AciI CCGC 5 cut(s) 42, 51, 54, 77, 686
AclWI GGATC 3 cut(s) 330, 343, 526
AcuI CTGAAG 3 cut(s) 533, 697, 1079
AfiI CCNNNNNNNGG 3 cut(s) 279, 282, 866
AgeI ACCGGT 1 cut(s) 366
AgsI TTSAA 7 cut(s) 230, 532, 553, 682, 829, 990, 1052
AjnI CCWGG 1 cut(s) 859
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 3 cut(s) 141, 394, 662
AlwI GGATC 3 cut(s) 330, 343, 526
AoxI GGCC 3 cut(s) 454, 546, 598
ApeKI GCWGC 5 cut(s) 85, 374, 921, 1007, 1132
ArsI GACNNNNNNTTYG 2 cut(s) 177, 209
AsiGI ACCGGT 1 cut(s) 366
AspS9I GGNCC 5 cut(s) 116, 364, 455, 854, 980
AvaII GGWCC 4 cut(s) 116, 364, 854, 980
BaeGI GKGCMC 1 cut(s) 415
BamHI GGATCC 1 cut(s) 335
BanI GGYRCC 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 134
BbvCI CCTCAGC 1 cut(s) 1164
BbvI GCAGC 5 cut(s) 97, 386, 908, 1019, 1144
BccI CCATC 3 cut(s) 326, 935, 1093
BceAI ACGGC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 861
BciVI GTATCC 1 cut(s) 279
BcoDI GTCTC 3 cut(s) 141, 394, 662
BfaI CTAG 4 cut(s) 356, 608, 701, 1179
BfmI CTRYAG 1 cut(s) 372
BfuI GTATCC 1 cut(s) 279
BisI GCNGC 8 cut(s) 43, 51, 54, 86, 375, 922, 1008, 1133
BlsI GCNGC 8 cut(s) 44, 52, 55, 87, 376, 923, 1009, 1134
Bme1390I CCNGG 1 cut(s) 861
Bme18I GGWCC 4 cut(s) 116, 364, 854, 980
BmgT120I GGNCC 5 cut(s) 116, 364, 455, 854, 980
BmiI GGNNCC 6 cut(s) 117, 337, 365, 414, 885, 981
BmrFI CCNGG 1 cut(s) 861
BmsI GCATC 3 cut(s) 163, 244, 600
BpmI CTGGAG 1 cut(s) 563
Bpu10I CCTNAGC 1 cut(s) 1164
BpuEI CTTGAG 1 cut(s) 145
BsaBI GATNNNNATC 1 cut(s) 757
BsaI GGTCTC 1 cut(s) 394
BsaJI CCNNGG 1 cut(s) 119
BsaWI WCCGGW 1 cut(s) 366
Bsc4I CCNNNNNNNGG 3 cut(s) 279, 282, 866
Bse118I RCCGGY 1 cut(s) 366
Bse3DI GCAATG 2 cut(s) 711, 1076
Bse8I GATNNNNATC 1 cut(s) 757
BseBI CCWGG 1 cut(s) 861
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 3 cut(s) 91, 178, 663
BseJI GATNNNNATC 1 cut(s) 757
BseLI CCNNNNNNNGG 3 cut(s) 279, 282, 866
BseMI GCAATG 2 cut(s) 711, 1076
BseMII CTCAG 2 cut(s) 800, 1178
BseRI GAGGAG 1 cut(s) 1152
BseSI GKGCMC 1 cut(s) 415
BseXI GCAGC 5 cut(s) 97, 386, 908, 1019, 1144
BseYI CCCAGC 1 cut(s) 962
BshFI GGCC 3 cut(s) 456, 548, 600
BshNI GGYRCC 1 cut(s) 412
BshTI ACCGGT 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 134
BsiSI CCGG 1 cut(s) 367
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 3 cut(s) 279, 282, 866
BsmAI GTCTC 3 cut(s) 141, 394, 662
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 3 cut(s) 456, 548, 600
Bso31I GGTCTC 1 cut(s) 394
Bsp1286I GDGCHC 2 cut(s) 134, 415
Bsp143I GATC 3 cut(s) 335, 518, 1084
BspACI CCGC 5 cut(s) 42, 51, 54, 77, 686
BspANI GGCC 3 cut(s) 456, 548, 600
BspCNI CTCAG 2 cut(s) 799, 1177
BspHI TCATGA 1 cut(s) 396
BspLI GGNNCC 6 cut(s) 117, 337, 365, 414, 885, 981
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 3 cut(s) 330, 343, 526
BspT107I GGYRCC 1 cut(s) 412
BspTNI GGTCTC 1 cut(s) 394
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 2 cut(s) 711, 1076
BsrFI RCCGGY 1 cut(s) 366
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 3 cut(s) 335, 518, 1084
Bst2UI CCWGG 1 cut(s) 861
Bst4CI ACNGT 3 cut(s) 129, 193, 1115
Bst6I CTCTTC 2 cut(s) 30, 743
BstAPI GCANNNNNTGC 2 cut(s) 740, 959
BstC8I GCNNGC 5 cut(s) 6, 134, 202, 598, 1170
BstDEI CTNAG 3 cut(s) 434, 786, 1164
BstF5I GGATG 3 cut(s) 91, 178, 663
BstKTI GATC 3 cut(s) 338, 521, 1087
BstMAI GTCTC 3 cut(s) 141, 394, 662
BstMBI GATC 3 cut(s) 335, 518, 1084
BstMWI GCNNNNNNNGC 9 cut(s) 263, 380, 419, 428, 545, 597, 620, 740, 959
BstNI CCWGG 1 cut(s) 861
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 1 cut(s) 859
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 415
BstV1I GCAGC 5 cut(s) 97, 386, 908, 1019, 1144
BstX2I RGATCY 2 cut(s) 335, 518
BstYI RGATCY 2 cut(s) 335, 518
BsuI GTATCC 1 cut(s) 279
BsuRI GGCC 3 cut(s) 456, 548, 600
BtgZI GCGATG 2 cut(s) 181, 577
BtsCI GGATG 3 cut(s) 91, 178, 663
Cac8I GCNNGC 5 cut(s) 6, 134, 202, 598, 1170
CciI TCATGA 1 cut(s) 396
Cfr10I RCCGGY 1 cut(s) 366
Cfr13I GGNCC 5 cut(s) 116, 364, 455, 854, 980
CspAI ACCGGT 1 cut(s) 366
CviAII CATG 5 cut(s) 107, 201, 397, 744, 1058
DdeI CTNAG 3 cut(s) 434, 786, 1164
DpnI GATC 3 cut(s) 337, 520, 1086
DpnII GATC 3 cut(s) 335, 518, 1084
DraI TTTAAA 1 cut(s) 757
Eam1104I CTCTTC 2 cut(s) 30, 743
EarI CTCTTC 2 cut(s) 30, 743
EciI GGCGGA 1 cut(s) 675
Eco147I AGGCCT 1 cut(s) 548
Eco31I GGTCTC 1 cut(s) 394
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 4 cut(s) 116, 364, 854, 980
Eco57I CTGAAG 3 cut(s) 533, 697, 1079
EcoO109I RGGNCCY 2 cut(s) 116, 854
EcoRII CCWGG 1 cut(s) 859
EcoRV GATATC 1 cut(s) 840
EcoT22I ATGCAT 1 cut(s) 593
FaeI CATG 5 cut(s) 110, 204, 400, 747, 1061
FalI AAGNNNNNCTT 2 cut(s) 471, 503
FaqI GGGAC 1 cut(s) 377
FatI CATG 5 cut(s) 106, 200, 396, 743, 1057
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 8 cut(s) 43, 51, 54, 86, 375, 922, 1008, 1133
FokI GGATG 3 cut(s) 78, 185, 670
Fsp4HI GCNGC 8 cut(s) 43, 51, 54, 86, 375, 922, 1008, 1133
FspBI CTAG 4 cut(s) 356, 608, 701, 1179
GluI GCNGC 8 cut(s) 43, 51, 54, 86, 375, 922, 1008, 1133
GsaI CCCAGC 1 cut(s) 966
GsuI CTGGAG 1 cut(s) 563
HaeIII GGCC 3 cut(s) 456, 548, 600
HapII CCGG 1 cut(s) 367
Hin1II CATG 5 cut(s) 110, 204, 400, 747, 1061
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HinfI GANTC 1 cut(s) 670
HpaII CCGG 1 cut(s) 367
Hpy166II GTNNAC 2 cut(s) 793, 1118
Hpy188I TCNGA 3 cut(s) 123, 1081, 1098
Hpy188III TCNNGA 6 cut(s) 356, 397, 542, 715, 1049, 1088
Hpy8I GTNNAC 2 cut(s) 793, 1118
HpyAV CCTTC 8 cut(s) 163, 462, 497, 508, 647, 699, 961, 1163
HpyCH4III ACNGT 3 cut(s) 129, 193, 1115
HpyCH4V TGCA 7 cut(s) 4, 85, 350, 374, 563, 591, 743
HpyF10VI GCNNNNNNNGC 9 cut(s) 263, 380, 419, 428, 545, 597, 620, 740, 959
HpyF3I CTNAG 3 cut(s) 434, 786, 1164
Hsp92II CATG 5 cut(s) 110, 204, 400, 747, 1061
Kzo9I GATC 3 cut(s) 335, 518, 1084
LmnI GCTCC 2 cut(s) 611, 1165
Lsp1109I GCAGC 5 cut(s) 97, 386, 908, 1019, 1144
LweI GCATC 3 cut(s) 163, 244, 600
MaeI CTAG 4 cut(s) 356, 608, 701, 1179
MaeIII GTNAC 1 cut(s) 908
MalI GATC 3 cut(s) 337, 520, 1086
MbiI CCGCTC 1 cut(s) 42
MboI GATC 3 cut(s) 335, 518, 1084
MboII GAAGA 6 cut(s) 44, 47, 491, 760, 812, 841
MfeI CAATTG 1 cut(s) 139
MflI RGATCY 2 cut(s) 335, 518
MhlI GDGCHC 2 cut(s) 134, 415
MluCI AATT 6 cut(s) 80, 139, 178, 318, 351, 832
MlyI GAGTC 1 cut(s) 664
MmeI TCCRAC 2 cut(s) 146, 847
MnlI CCTC 7 cut(s) 99, 129, 538, 845, 867, 897, 1173
Mph1103I ATGCAT 1 cut(s) 593
MseI TTAA 5 cut(s) 62, 294, 317, 756, 848
MslI CAYNNNNRTG 1 cut(s) 894
MspA1I CMGCKG 1 cut(s) 1135
MspI CCGG 1 cut(s) 367
MspR9I CCNGG 1 cut(s) 861
MunI CAATTG 1 cut(s) 139
MvaI CCWGG 1 cut(s) 861
MwoI GCNNNNNNNGC 9 cut(s) 263, 380, 419, 428, 545, 597, 620, 740, 959
NdeII GATC 3 cut(s) 335, 518, 1084
NlaIII CATG 5 cut(s) 110, 204, 400, 747, 1061
NlaIV GGNNCC 6 cut(s) 117, 337, 365, 414, 885, 981
NmuCI GTSAC 1 cut(s) 908
NsiI ATGCAT 1 cut(s) 593
NspI RCATGY 1 cut(s) 204
PaeI GCATGC 1 cut(s) 204
PagI TCATGA 1 cut(s) 396
PceI AGGCCT 1 cut(s) 548
PflMI CCANNNNNTGG 1 cut(s) 282
PinAI ACCGGT 1 cut(s) 366
PkrI GCNGC 8 cut(s) 44, 52, 55, 87, 376, 923, 1009, 1134
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
PpuMI RGGWCCY 2 cut(s) 116, 854
Psp5II RGGWCCY 2 cut(s) 116, 854
Psp6I CCWGG 1 cut(s) 859
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 1 cut(s) 859
PspN4I GGNNCC 6 cut(s) 117, 337, 365, 414, 885, 981
PspPI GGNCC 5 cut(s) 116, 364, 455, 854, 980
PspPPI RGGWCCY 2 cut(s) 116, 854
PstI CTGCAG 1 cut(s) 376
PsuI RGATCY 2 cut(s) 335, 518
PvuII CAGCTG 1 cut(s) 1135
RseI CAYNNNNRTG 1 cut(s) 894
SalI GTCGAC 1 cut(s) 791
SaqAI TTAA 5 cut(s) 62, 294, 317, 756, 848
SatI GCNGC 8 cut(s) 43, 51, 54, 86, 375, 922, 1008, 1133
Sau3AI GATC 3 cut(s) 335, 518, 1084
Sau96I GGNCC 5 cut(s) 116, 364, 455, 854, 980
SchI GAGTC 1 cut(s) 664
ScrFI CCNGG 1 cut(s) 861
SduI GDGCHC 2 cut(s) 134, 415
SfaNI GCATC 3 cut(s) 163, 244, 600
SfcI CTRYAG 1 cut(s) 372
SinI GGWCC 4 cut(s) 116, 364, 854, 980
SmiI ATTTAAAT 1 cut(s) 757
SmiMI CAYNNNNRTG 1 cut(s) 894
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
SphI GCATGC 1 cut(s) 204
Sse9I AATT 6 cut(s) 80, 139, 178, 318, 351, 832
SseBI AGGCCT 1 cut(s) 548
SsiI CCGC 5 cut(s) 42, 51, 54, 77, 686
SspI AATATT 1 cut(s) 721
SspMI CTAG 4 cut(s) 356, 608, 701, 1179
StuI AGGCCT 1 cut(s) 548
StyD4I CCNGG 1 cut(s) 859
SwaI ATTTAAAT 1 cut(s) 757
TaaI ACNGT 3 cut(s) 129, 193, 1115
TaqI TCGA 1 cut(s) 792
TaqII GACCGA 1 cut(s) 418
TasI AATT 6 cut(s) 80, 139, 178, 318, 351, 832
TauI GCSGC 3 cut(s) 45, 53, 56
Tru1I TTAA 5 cut(s) 62, 294, 317, 756, 848
Tru9I TTAA 5 cut(s) 62, 294, 317, 756, 848
TseFI GTSAC 1 cut(s) 908
TseI GCWGC 5 cut(s) 85, 374, 921, 1007, 1132
Tsp45I GTSAC 1 cut(s) 908
TspDTI ATGAA 4 cut(s) 95, 196, 339, 813
Van91I CCANNNNNTGG 1 cut(s) 282
VpaK11BI GGWCC 4 cut(s) 116, 364, 854, 980
XbaI TCTAGA 1 cut(s) 355
XceI RCATGY 1 cut(s) 204
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 4 cut(s) 356, 608, 701, 1179
Zsp2I ATGCAT 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.