pycom17g13030

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012134
Physical Location & Seq
Forward (+)
10710270 .. 10710998
729 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g13030.1

Sequence Viewer

Length: 729 bp
ATGAGAAGGGATATTGAAGATGACCTTCGTTTTCTTGCTTGGGAGAGCTTGAAGGGTTCTGTGAGGTTTAAAACAGCTATGGAGGCTTTGAAATCAGTTGCTGAACGGTATGGAAGCACTAATGTGTGTATTGCAGGACATTCATTGGGGGCCGGATTTGCTCTCCAAGTGGGAAAAGCATTGGCCAAAGAAGGGATGTATGTGGAGACTCATCTGTTCAACCCGCCTTCTGTGTCGCTAGCAATGAGCTTCAGAAATATTGGAGAGAAAGCAGGATTTGCTTGGAAGAGATTTAAGTCAATGCTCCCTTCGGGCAGTGGAACTCAGGTCAGCAGTGAAGAGGAGGGGGATAAAGTTCCTGGTTTTGCATTGAAGGATTGGATACCAAATTTTTCAGGTCTGAAGAATCCTGGTGTAGGCTTGGGAAAATGGGTTCCTCATTTATATGTAAATAACAGTGACTATATCTGCTGCTCTTACACTGAACATGATGGAATAGAAGGAGCCAATGCCGACAAGGAGACTGTGCGTCCAACAAACGGGCAAGTAGCAGCGAAGCTGTTTGTGATGTCAAAAGGAAAACAGAAGTTTCTGGAGGCACACGGGTTAGAGCAATGGTGGTCGGATGACCTTGAACTTCAACTGGCTATGCATAACAGTAAGCTCATTAGCAGGCAACTGAAATCCTTGCATAGCGTTCCAGCTCCACAACAAACGCAAGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

243

Amino Acids

26.72

Weight (kDa)

8.67

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50890
fragaria_vesca FvH4_6g38560
malus_domestica MD09G1149800.v1.1 MD17G1136700.v1.1
prunus_persica Prupe.3G176300_v2.0.a1
pyrus_communis pycom09g06920 pycom17g13030
rosa_chinensis RchiOBHm_Chr2g0151971
rosa_laevigata RLG00000020595
rosa_multiflora Rmu_sc0005294.1_g000001 Rmu_sc0023925.1_g000001
rosa_roxburghii Rroxscaffold_2G00096090
rosa_rugosa Rorug02G0430000
rosa_samantha Rh2AG490600 Rh2BG502900 Rh2CG477000 Rh2DG514100
rosa_wichuraiana Rw0G020220 Rw2G040320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 2 cut(s) 235, 422
AfiI CCNNNNNNNGG 3 cut(s) 192, 416, 539
AgsI TTSAA 7 cut(s) 17, 52, 91, 220, 373, 635, 641
AhdI GACNNNNNGTC 1 cut(s) 528
AjnI CCWGG 2 cut(s) 358, 409
AleI CACNNNNGTG 1 cut(s) 122
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 7 cut(s) 48, 77, 249, 559, 664, 704, 722
AluI AGCT 7 cut(s) 48, 77, 249, 559, 664, 704, 722
Alw26I GTCTC 2 cut(s) 200, 515
AlwNI CAGNNNCTG 1 cut(s) 101
AoxI GGCC 2 cut(s) 150, 183
ApeKI GCWGC 2 cut(s) 471, 551
ApoI RAATTY 1 cut(s) 388
AspS9I GGNCC 1 cut(s) 150
AsuNHI GCTAGC 1 cut(s) 238
BalI TGGCCA 1 cut(s) 185
BbvI GCAGC 2 cut(s) 458, 563
BccI CCATC 1 cut(s) 485
BciT130I CCWGG 2 cut(s) 360, 411
BciVI GTATCC 1 cut(s) 375
BcoDI GTCTC 2 cut(s) 200, 515
BfaI CTAG 2 cut(s) 239, 723
BfuI GTATCC 1 cut(s) 375
BisI GCNGC 2 cut(s) 472, 552
BlsI GCNGC 2 cut(s) 473, 553
Bme1390I CCNGG 2 cut(s) 360, 411
BmeRI GACNNNNNGTC 1 cut(s) 528
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 3 cut(s) 151, 435, 505
BmrFI CCNGG 2 cut(s) 360, 411
BmtI GCTAGC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 614
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 416, 539
Bse1I ACTGG 1 cut(s) 648
Bse3DI GCAATG 2 cut(s) 249, 620
BseBI CCWGG 2 cut(s) 360, 411
BseGI GGATG 2 cut(s) 201, 631
BseLI CCNNNNNNNGG 3 cut(s) 192, 416, 539
BseMI GCAATG 2 cut(s) 249, 620
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 1 cut(s) 648
BseRI GAGGAG 1 cut(s) 356
BseXI GCAGC 2 cut(s) 458, 563
BshFI GGCC 2 cut(s) 152, 185
BsiSI CCGG 1 cut(s) 153
BslI CCNNNNNNNGG 3 cut(s) 192, 416, 539
BsmAI GTCTC 2 cut(s) 200, 515
BsnI GGCC 2 cut(s) 152, 185
BspACI CCGC 1 cut(s) 224
BspANI GGCC 2 cut(s) 152, 185
BspCNI CTCAG 1 cut(s) 337
BspLI GGNNCC 3 cut(s) 151, 435, 505
BspOI GCTAGC 1 cut(s) 242
BsrDI GCAATG 2 cut(s) 249, 620
BsrI ACTGG 1 cut(s) 648
Bst2UI CCWGG 2 cut(s) 360, 411
Bst4CI ACNGT 4 cut(s) 108, 458, 526, 659
Bst6I CTCTTC 2 cut(s) 281, 333
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 3 cut(s) 240, 674, 720
BstDEI CTNAG 1 cut(s) 324
BstENI CCTNNNNNAGG 1 cut(s) 414
BstF5I GGATG 2 cut(s) 201, 631
BstMAI GTCTC 2 cut(s) 200, 515
BstMWI GCNNNNNNNGC 3 cut(s) 83, 158, 278
BstNI CCWGG 2 cut(s) 360, 411
BstSCI CCNGG 2 cut(s) 358, 409
BstV1I GCAGC 2 cut(s) 458, 563
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 2 cut(s) 152, 185
BtsCI GGATG 2 cut(s) 201, 631
BtsI GCAGTG 2 cut(s) 322, 340
BtsIMutI CAGTG 4 cut(s) 322, 340, 463, 480
Cac8I GCNNGC 3 cut(s) 240, 674, 720
CaiI CAGNNNCTG 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 150
CseI GACGC 1 cut(s) 518
CviAII CATG 1 cut(s) 488
DdeI CTNAG 1 cut(s) 324
DraI TTTAAA 1 cut(s) 70
DriI GACNNNNNGTC 1 cut(s) 528
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 2 cut(s) 281, 333
Eam1105I GACNNNNNGTC 1 cut(s) 528
EarI CTCTTC 2 cut(s) 281, 333
Eco57I CTGAAG 2 cut(s) 235, 422
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoRII CCWGG 2 cut(s) 358, 409
EcoT22I ATGCAT 1 cut(s) 654
FaeI CATG 1 cut(s) 491
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FatI CATG 1 cut(s) 487
FauI CCCGC 1 cut(s) 231
Fnu4HI GCNGC 2 cut(s) 472, 552
FokI GGATG 2 cut(s) 208, 638
Fsp4HI GCNGC 2 cut(s) 472, 552
FspBI CTAG 2 cut(s) 239, 723
GluI GCNGC 2 cut(s) 472, 552
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 2 cut(s) 152, 185
HapII CCGG 1 cut(s) 153
HgaI GACGC 1 cut(s) 518
Hin1II CATG 1 cut(s) 491
HinfI GANTC 2 cut(s) 208, 406
HpaII CCGG 1 cut(s) 153
Hpy188I TCNGA 3 cut(s) 254, 402, 625
Hpy188III TCNNGA 1 cut(s) 593
HpyAV CCTTC 7 cut(s) 35, 46, 185, 237, 318, 367, 494
HpyCH4III ACNGT 4 cut(s) 108, 458, 526, 659
HpyCH4V TGCA 4 cut(s) 134, 368, 652, 691
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 158, 278
HpyF3I CTNAG 1 cut(s) 324
Hsp92II CATG 1 cut(s) 491
LmnI GCTCC 3 cut(s) 309, 503, 709
Lsp1109I GCAGC 2 cut(s) 458, 563
MaeI CTAG 2 cut(s) 239, 723
MaeIII GTNAC 1 cut(s) 458
MboII GAAGA 4 cut(s) 29, 298, 350, 415
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 1 cut(s) 388
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 1 cut(s) 202
MmeI TCCRAC 2 cut(s) 557, 603
MnlI CCTC 6 cut(s) 57, 76, 334, 337, 447, 589
Mox20I TGGCCA 1 cut(s) 185
Mph1103I ATGCAT 1 cut(s) 654
MscI TGGCCA 1 cut(s) 185
MseI TTAA 2 cut(s) 69, 294
MslI CAYNNNNRTG 2 cut(s) 122, 444
Msp20I TGGCCA 1 cut(s) 185
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 2 cut(s) 360, 411
MvaI CCWGG 2 cut(s) 360, 411
MwoI GCNNNNNNNGC 3 cut(s) 83, 158, 278
NheI GCTAGC 1 cut(s) 238
NlaIII CATG 1 cut(s) 491
NlaIV GGNNCC 3 cut(s) 151, 435, 505
NmuCI GTSAC 1 cut(s) 458
NsiI ATGCAT 1 cut(s) 654
OliI CACNNNNGTG 1 cut(s) 122
PfeI GAWTC 1 cut(s) 406
PkrI GCNGC 2 cut(s) 473, 553
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
Psp6I CCWGG 2 cut(s) 358, 409
PspGI CCWGG 2 cut(s) 358, 409
PspN4I GGNNCC 3 cut(s) 151, 435, 505
PspPI GGNCC 1 cut(s) 150
PstNI CAGNNNCTG 1 cut(s) 101
RseI CAYNNNNRTG 2 cut(s) 122, 444
SaqAI TTAA 2 cut(s) 69, 294
SatI GCNGC 2 cut(s) 472, 552
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 2 cut(s) 360, 411
SmiMI CAYNNNNRTG 2 cut(s) 122, 444
Sse9I AATT 1 cut(s) 388
SsiI CCGC 1 cut(s) 224
SspI AATATT 1 cut(s) 259
SspMI CTAG 2 cut(s) 239, 723
StyD4I CCNGG 2 cut(s) 358, 409
TaaI ACNGT 4 cut(s) 108, 458, 526, 659
TasI AATT 1 cut(s) 388
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 2 cut(s) 69, 294
Tru9I TTAA 2 cut(s) 69, 294
TscAI CASTG 4 cut(s) 322, 340, 463, 487
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 2 cut(s) 471, 551
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 1 cut(s) 132
TspRI CASTG 4 cut(s) 322, 340, 463, 487
XagI CCTNNNNNAGG 1 cut(s) 414
XapI RAATTY 1 cut(s) 388
XspI CTAG 2 cut(s) 239, 723
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.