Prupe.3G176300_v2.0.a1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0012134
Physical Location & Seq
Reverse (-)
19359474 .. 19362006
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G176300.1

Sequence Viewer

Length: 1239 bp
ATGGAAAGAAACCCAAAACAAAGGGAGGAAGAAACTGAAGAAAGAGGCGGTGGCGGTGCTGGTGGTGTCGGTGCATTATTAATGGCCGAAGGCAGTGCAGAGGAGATAGCCAAGGAGATTGTGGCAGCAGAGGAGGCTCCTCATCCGTACGCTTTTCATGTATCTGGACCTCGAAATTTGACTGCTCCGAATTGGAGGGACCTTATAAACTCTAGTTGGAAGGACGGGAATTATAAACGAACTGTAATCGCATGCTTCATACAAGCAATATATTTGCTTGAACTCGACAGACAAGAGAATAGAGATGAAGCAAATGCTCTAGCTCCTAAGTGGTGGATACCCTTTAAGTATAAACTATCCGAAACCTTAATCGATGAAAGAGATGGATCCATATTTGGGGCAATTTTGGAATGGGATCGATCTGCAGCACTTGCTGACTTTGTACTCATCAGACCAAGCGGTGCACCAAAGGCGGTTCTAGCACTTAGAGGAACATTGCTCAAAGGCCCAACTATGCGAAGGGATATTGAAGACGACCTTCGTTTTCTTGCTTGGGAGAGCTTGAAGGGCTCTGTTAGGTTTAAAGCAGCTATGGAGGCTTTGAAATCAGTTGCTGAAAGGTATGGAAGTAGTAATGTTTGTATTGCGGGACACTCATTGGGGGCTGGATTTGCTCTCCAAGTGGGAAAAGCATTAGCCAAAGAAGGGATGTATGTGGAAACTCATTTGTTCAATCCGCCTTCTGTTTCGCTAGCAATGAGCTTCAGAAATATCGGAGAGAAAGCAGGATTTGCTTGGAAAAGATTTAAGTCAATGCTCCCTTTGAGAAGTGGAGCTCAGGTCAGCAGTGAAGAAGGGGATAGGACTTCTGGTTTTGGATTGAAGAATTGGATGCCAAATTTTTCGGGTTTGAAGAATCCTGGTATGGGTTTGGGAAAATGGGTTCCTCATTTGTATGTAAATAACAGTGACTACATCTGCTGTTCTTATACTGAGCATGATGGAGGAGAAGTAAACAATGCTAACAAGGAGAATGTGCGTCCAACAAATGGTCAAGTGGCAGCAAAGCTGTTTGTGATGTCAAAGGGAAACCAGAAGTTTTCGGAGGCTCACGGTTTAGAGCAATGGTGGTCTGATGATTTGGAACTTCAACTGGCTATGCATAACAGTAAGCTCATTAGCAGGCAGCTGAAATCCTTGTATAGCCTTCCAGCTCCACAACAAACACTTGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

413

Amino Acids

45.59

Weight (kDa)

7.68

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50890
fragaria_vesca FvH4_6g38560
malus_domestica MD09G1149800.v1.1 MD17G1136700.v1.1
prunus_persica Prupe.3G176300_v2.0.a1
pyrus_communis pycom09g06920 pycom17g13030
rosa_chinensis RchiOBHm_Chr2g0151971
rosa_laevigata RLG00000020595
rosa_multiflora Rmu_sc0005294.1_g000001 Rmu_sc0023925.1_g000001
rosa_roxburghii Rroxscaffold_2G00096090
rosa_rugosa Rorug02G0430000
rosa_samantha Rh2AG490600 Rh2BG502900 Rh2CG477000 Rh2DG514100
rosa_wichuraiana Rw0G020220 Rw2G040320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 206, 234
AccB7I CCANNNNNTGG 1 cut(s) 1049
AciI CCGC 6 cut(s) 48, 54, 459, 473, 647, 737
AclWI GGATC 3 cut(s) 381, 394, 423
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 2 cut(s) 175, 898
AcuI CTGAAG 2 cut(s) 57, 748
AfaI GTAC 2 cut(s) 149, 444
AfiI CCNNNNNNNGG 4 cut(s) 396, 705, 926, 1049
AgsI TTSAA 8 cut(s) 281, 530, 565, 604, 733, 883, 913, 1151
AjnI CCWGG 1 cut(s) 919
AloI GAACNNNNNNTCC 2 cut(s) 927, 959
AluBI AGCT 9 cut(s) 323, 561, 590, 762, 836, 1069, 1174, 1189, 1214
AluI AGCT 9 cut(s) 323, 561, 590, 762, 836, 1069, 1174, 1189, 1214
Alw21I GWGCWC 2 cut(s) 466, 838
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 3 cut(s) 381, 394, 423
AlwNI CAGNNNCTG 1 cut(s) 614
AoxI GGCC 2 cut(s) 84, 505
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 5 cut(s) 125, 425, 587, 1061, 1186
ApoI RAATTY 2 cut(s) 175, 898
AseI ATTAAT 1 cut(s) 80
AspS9I GGNCC 3 cut(s) 167, 199, 506
AsuNHI GCTAGC 1 cut(s) 751
AvaII GGWCC 2 cut(s) 167, 199
BaeGI GKGCMC 1 cut(s) 466
BamHI GGATCC 1 cut(s) 386
BanII GRGCYC 2 cut(s) 572, 838
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 2 cut(s) 466, 838
BbvI GCAGC 5 cut(s) 137, 437, 599, 1073, 1198
BccI CCATC 2 cut(s) 377, 995
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 1 cut(s) 921
BciVI GTATCC 1 cut(s) 330
BfaI CTAG 5 cut(s) 213, 320, 479, 752, 1233
BfmI CTRYAG 1 cut(s) 423
BfuI GTATCC 1 cut(s) 330
BisI GCNGC 5 cut(s) 126, 426, 588, 1062, 1187
BlsI GCNGC 5 cut(s) 127, 427, 589, 1063, 1188
Bme1390I CCNGG 1 cut(s) 921
Bme18I GGWCC 2 cut(s) 167, 199
BmgT120I GGNCC 3 cut(s) 167, 199, 506
BmiI GGNNCC 4 cut(s) 138, 200, 388, 945
BmrFI CCNGG 1 cut(s) 921
BmsI GCATC 1 cut(s) 882
BmtI GCTAGC 1 cut(s) 755
BpiI GAAGAC 1 cut(s) 537
Bpu10I CCTNAGC 1 cut(s) 837
Bsa29I ATCGAT 2 cut(s) 372, 418
BsaJI CCNNGG 1 cut(s) 111
BsaXI ACNNNNNCTCC 4 cut(s) 825, 855, 996, 1026
Bsc4I CCNNNNNNNGG 4 cut(s) 396, 705, 926, 1049
Bse1I ACTGG 1 cut(s) 1158
Bse3DI GCAATG 3 cut(s) 494, 762, 1130
BseBI CCWGG 1 cut(s) 921
BseCI ATCGAT 2 cut(s) 372, 418
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 3 cut(s) 142, 714, 897
BseLI CCNNNNNNNGG 4 cut(s) 396, 705, 926, 1049
BseMI GCAATG 3 cut(s) 494, 762, 1130
BseMII CTCAG 2 cut(s) 851, 984
BseNI ACTGG 1 cut(s) 1158
BseRI GAGGAG 4 cut(s) 116, 129, 146, 1020
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 5 cut(s) 137, 437, 599, 1073, 1198
BsgI GTGCAG 1 cut(s) 117
BshFI GGCC 2 cut(s) 86, 507
BshVI ATCGAT 2 cut(s) 372, 418
BsiHKAI GWGCWC 2 cut(s) 466, 838
BsiWI CGTACG 1 cut(s) 147
BslFI GGGAC 2 cut(s) 212, 663
BslI CCNNNNNNNGG 4 cut(s) 396, 705, 926, 1049
BsmFI GGGAC 2 cut(s) 212, 663
BsnI GGCC 2 cut(s) 86, 507
Bsp1286I GDGCHC 3 cut(s) 466, 572, 838
Bsp143I GATC 3 cut(s) 386, 415, 419
BspACI CCGC 6 cut(s) 48, 54, 459, 473, 647, 737
BspANI GGCC 2 cut(s) 86, 507
BspCNI CTCAG 2 cut(s) 850, 985
BspDI ATCGAT 2 cut(s) 372, 418
BspLI GGNNCC 4 cut(s) 138, 200, 388, 945
BspMAI CTGCAG 1 cut(s) 427
BspOI GCTAGC 1 cut(s) 755
BspPI GGATC 3 cut(s) 381, 394, 423
BsrDI GCAATG 3 cut(s) 494, 762, 1130
BsrI ACTGG 1 cut(s) 1158
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 3 cut(s) 386, 415, 419
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 921
Bst4CI ACNGT 4 cut(s) 244, 968, 1115, 1169
BstAPI GCANNNNNTGC 2 cut(s) 431, 791
BstC8I GCNNGC 3 cut(s) 253, 753, 1184
BstDEI CTNAG 4 cut(s) 327, 485, 837, 993
BstF5I GGATG 3 cut(s) 142, 714, 897
BstKTI GATC 3 cut(s) 389, 418, 422
BstMBI GATC 3 cut(s) 386, 415, 419
BstMWI GCNNNNNNNGC 8 cut(s) 134, 431, 470, 479, 567, 596, 671, 791
BstNI CCWGG 1 cut(s) 921
BstNSI RCATGY 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 919
BstSFI CTRYAG 1 cut(s) 423
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 5 cut(s) 137, 437, 599, 1073, 1198
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
Bsu15I ATCGAT 2 cut(s) 372, 418
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 2 cut(s) 86, 507
BsuTUI ATCGAT 2 cut(s) 372, 418
BtsCI GGATG 3 cut(s) 142, 714, 897
BtsI GCAGTG 2 cut(s) 100, 853
BtsIMutI CAGTG 3 cut(s) 100, 853, 973
Cac8I GCNNGC 3 cut(s) 253, 753, 1184
CaiI CAGNNNCTG 1 cut(s) 614
Cfr13I GGNCC 3 cut(s) 167, 199, 506
ClaI ATCGAT 2 cut(s) 372, 418
CseI GACGC 1 cut(s) 1028
Csp6I GTAC 2 cut(s) 148, 443
CviAII CATG 3 cut(s) 158, 252, 998
CviQI GTAC 2 cut(s) 148, 443
DdeI CTNAG 4 cut(s) 327, 485, 837, 993
DpnI GATC 3 cut(s) 388, 417, 421
DpnII GATC 3 cut(s) 386, 415, 419
DraI TTTAAA 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 84
EciI GGCGGA 1 cut(s) 726
Ecl136II GAGCTC 1 cut(s) 836
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 2 cut(s) 572, 838
Eco47I GGWCC 2 cut(s) 167, 199
Eco53kI GAGCTC 1 cut(s) 836
Eco57I CTGAAG 2 cut(s) 57, 748
EcoICRI GAGCTC 1 cut(s) 836
EcoO109I RGGNCCY 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 919
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 2 cut(s) 572, 838
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 3 cut(s) 161, 255, 1001
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 2 cut(s) 212, 663
FatI CATG 3 cut(s) 157, 251, 997
FauI CCCGC 1 cut(s) 640
Fnu4HI GCNGC 5 cut(s) 126, 426, 588, 1062, 1187
FokI GGATG 3 cut(s) 129, 721, 904
FriOI GRGCYC 2 cut(s) 572, 838
Fsp4HI GCNGC 5 cut(s) 126, 426, 588, 1062, 1187
FspBI CTAG 5 cut(s) 213, 320, 479, 752, 1233
GluI GCNGC 5 cut(s) 126, 426, 588, 1062, 1187
HaeIII GGCC 2 cut(s) 86, 507
HgaI GACGC 1 cut(s) 1028
Hin1II CATG 3 cut(s) 161, 255, 1001
HinfI GANTC 1 cut(s) 916
Hpy166II GTNNAC 2 cut(s) 464, 1015
Hpy188I TCNGA 7 cut(s) 189, 361, 452, 767, 776, 1105, 1135
Hpy188III TCNNGA 1 cut(s) 165
Hpy8I GTNNAC 2 cut(s) 464, 1015
HpyAV CCTTC 9 cut(s) 83, 214, 513, 548, 559, 698, 750, 848, 1217
HpyCH4III ACNGT 4 cut(s) 244, 968, 1115, 1169
HpyCH4V TGCA 5 cut(s) 74, 98, 425, 464, 1162
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 431, 470, 479, 567, 596, 671, 791
HpyF3I CTNAG 4 cut(s) 327, 485, 837, 993
Hsp92II CATG 3 cut(s) 161, 255, 1001
Kzo9I GATC 3 cut(s) 386, 415, 419
LmnI GCTCC 6 cut(s) 142, 190, 328, 822, 833, 1219
Lsp1109I GCAGC 5 cut(s) 137, 437, 599, 1073, 1198
LweI GCATC 1 cut(s) 882
MaeI CTAG 5 cut(s) 213, 320, 479, 752, 1233
MaeIII GTNAC 1 cut(s) 968
MalI GATC 3 cut(s) 388, 417, 421
MboI GATC 3 cut(s) 386, 415, 419
MboII GAAGA 6 cut(s) 41, 50, 542, 863, 895, 925
MflI RGATCY 1 cut(s) 386
MhlI GDGCHC 3 cut(s) 466, 572, 838
MluCI AATT 6 cut(s) 175, 190, 229, 402, 886, 898
MmeI TCCRAC 2 cut(s) 197, 1067
Mph1103I ATGCAT 1 cut(s) 1164
MseI TTAA 5 cut(s) 80, 345, 368, 582, 807
MslI CAYNNNNRTG 1 cut(s) 954
MspA1I CMGCKG 1 cut(s) 1189
MspR9I CCNGG 1 cut(s) 921
MvaI CCWGG 1 cut(s) 921
MwoI GCNNNNNNNGC 8 cut(s) 134, 431, 470, 479, 567, 596, 671, 791
NdeII GATC 3 cut(s) 386, 415, 419
NheI GCTAGC 1 cut(s) 751
NlaIII CATG 3 cut(s) 161, 255, 1001
NlaIV GGNNCC 4 cut(s) 138, 200, 388, 945
NmuCI GTSAC 1 cut(s) 968
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 1 cut(s) 255
PaeI GCATGC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 916
Pfl23II CGTACG 1 cut(s) 147
PflMI CCANNNNNTGG 1 cut(s) 1049
PkrI GCNGC 5 cut(s) 127, 427, 589, 1063, 1188
PpuMI RGGWCCY 1 cut(s) 199
PshBI ATTAAT 1 cut(s) 80
PsiI TTATAA 2 cut(s) 206, 234
Psp124BI GAGCTC 1 cut(s) 838
Psp5II RGGWCCY 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 919
PspGI CCWGG 1 cut(s) 919
PspLI CGTACG 1 cut(s) 147
PspN4I GGNNCC 4 cut(s) 138, 200, 388, 945
PspPI GGNCC 3 cut(s) 167, 199, 506
PspPPI RGGWCCY 1 cut(s) 199
PstI CTGCAG 1 cut(s) 427
PstNI CAGNNNCTG 1 cut(s) 614
PsuI RGATCY 1 cut(s) 386
PvuII CAGCTG 1 cut(s) 1189
RsaI GTAC 2 cut(s) 149, 444
RsaNI GTAC 2 cut(s) 148, 443
RseI CAYNNNNRTG 1 cut(s) 954
SacI GAGCTC 1 cut(s) 838
SaqAI TTAA 5 cut(s) 80, 345, 368, 582, 807
SatI GCNGC 5 cut(s) 126, 426, 588, 1062, 1187
Sau3AI GATC 3 cut(s) 386, 415, 419
Sau96I GGNCC 3 cut(s) 167, 199, 506
ScrFI CCNGG 1 cut(s) 921
SduI GDGCHC 3 cut(s) 466, 572, 838
SfaNI GCATC 1 cut(s) 882
SfcI CTRYAG 1 cut(s) 423
SinI GGWCC 2 cut(s) 167, 199
SmiMI CAYNNNNRTG 1 cut(s) 954
SphI GCATGC 1 cut(s) 255
Sse9I AATT 6 cut(s) 175, 190, 229, 402, 886, 898
SsiI CCGC 6 cut(s) 48, 54, 459, 473, 647, 737
SspMI CTAG 5 cut(s) 213, 320, 479, 752, 1233
SstI GAGCTC 1 cut(s) 838
StyD4I CCNGG 1 cut(s) 919
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 4 cut(s) 244, 968, 1115, 1169
TaqI TCGA 4 cut(s) 172, 285, 372, 418
TasI AATT 6 cut(s) 175, 190, 229, 402, 886, 898
TatI WGTACW 1 cut(s) 442
TfiI GAWTC 1 cut(s) 916
Tru1I TTAA 5 cut(s) 80, 345, 368, 582, 807
Tru9I TTAA 5 cut(s) 80, 345, 368, 582, 807
TscAI CASTG 3 cut(s) 100, 853, 973
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 5 cut(s) 125, 425, 587, 1061, 1186
Tsp45I GTSAC 1 cut(s) 968
TspDTI ATGAA 4 cut(s) 146, 247, 321, 390
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 3 cut(s) 100, 853, 973
Van91I CCANNNNNTGG 1 cut(s) 1049
VneI GTGCAC 1 cut(s) 462
VpaK11BI GGWCC 2 cut(s) 167, 199
VspI ATTAAT 1 cut(s) 80
XapI RAATTY 2 cut(s) 175, 898
XceI RCATGY 1 cut(s) 255
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XspI CTAG 5 cut(s) 213, 320, 479, 752, 1233
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.