MD09G1149800.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012134
Physical Location & Seq
Forward (+)
11756474 .. 11758918
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1149800.v1.1.491

Sequence Viewer

Length: 1236 bp
ATGCAAAGAAACCCAGAACAAAGAGAGGAAGAAGAAGCAGCAGAAAGAGGCGGTGCTGTTTCTGGTGGCGGTGCATTAATGACTGAGCACGGCGGCGGAGAGGAGATATCTAAGGAAGTTGTGGCAGCAGAGGCTGCTCATCCATACGCTTTCCATGTATCCGGACCTCGCAATTTGACCTCTCCGAATTGGAGAGACCTTCTAAACTCGAGTTGGAAGGACGGGAATTATAAACGAACTGTAATCGCGTGCTTCATACAATCAATATATTTGCTTGAACTCGACAGACAAGAGAATAGAGATGAAGCACATGCTCTAGCTCCTAAATGGTGGATACCCTTTAAGTATAAATTGTCGCAAACCTTAATCGATGAAAGAGATGGATCCATATTTGGGGCAATACTGGAATGGGATCGATCTGCAGCACTTGCTGACTTTGTACTTATCAGACCAAGCGGAGCGCCAAAGGTGGTTCTAGCACTTCGAGGAACATTACTCAAAGGTCCAACTATGAGAAGGGATATTGAAGACGACCTTCGTTTTCTTGCTTGGGAGAGCTTGAAGGGTTCTGTGAGGTTCAAAACAGCTATGGAAGCTTTGAAATCAGTTGCTGAACGGTATGGAAGCAGTAATGTGTGTATTGCAGGACACTCATTGGGGGCCGGATTTGCTCTCCAAGTAGGAAAAGCATTAGCCAAAGAAGGGATGTATGTGGAGACTCATCTGTTCAACCCGCCTTCCGTATCGCTAGCAATGAGCTTCAGAAATATTGGAGAGAAAGCAGGGTTTGCTTGGAAGAGGTTTAAGTCAATGCTCCCTTTGGGCAGTGCAACTAAGGCCAGCAGTGAAGAGGGGGATAATGCTCCTGGTTCTGCATTGAAGAATTGGATACCAAATTTTTCGGGTTTGAAGAATCCTGGTGTAGGCTTGGGAAAATGGGTTCCTCATTTATACGTAAATAATAGTGACTATATCTGCTGCTCTTACACCGAACATGATGGAATAGAAGGAGCCAATGCCGACAAAGAGAATGTGCGTCCAACCAACGGGCAAGTAGCAGCGAAGCTGTTTGTGATGTCCAAAGGAAACCAGAAGTTTCTGGAGGCTCACGGGTTAGAGCAATGGTGGTCGGATGACTTGGAACTTCAACTGGCTATGCATAACAGTAAGCTTATTAGCAGACAACTGAAATCCTTGTATTCCCTTCCAGCTCCACAACAAACACAAGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

412

Amino Acids

45.35

Weight (kDa)

7.16

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50890
fragaria_vesca FvH4_6g38560
malus_domestica MD09G1149800.v1.1 MD17G1136700.v1.1
prunus_persica Prupe.3G176300_v2.0.a1
pyrus_communis pycom09g06920 pycom17g13030
rosa_chinensis RchiOBHm_Chr2g0151971
rosa_laevigata RLG00000020595
rosa_multiflora Rmu_sc0005294.1_g000001 Rmu_sc0023925.1_g000001
rosa_roxburghii Rroxscaffold_2G00096090
rosa_rugosa Rorug02G0430000
rosa_samantha Rh2AG490600 Rh2BG502900 Rh2CG477000 Rh2DG514100
rosa_wichuraiana Rw0G020220 Rw2G040320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
AccII CGCG 1 cut(s) 248
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 6 cut(s) 51, 69, 93, 96, 456, 734
AclWI GGATC 3 cut(s) 378, 391, 420
AcsI RAATTY 1 cut(s) 895
AcuI CTGAAG 1 cut(s) 745
AfaI GTAC 1 cut(s) 441
AfiI CCNNNNNNNGG 4 cut(s) 393, 702, 923, 1046
AgsI TTSAA 9 cut(s) 278, 527, 562, 580, 601, 730, 880, 910, 1148
AjnI CCWGG 2 cut(s) 865, 916
AloI GAACNNNNNNTCC 2 cut(s) 924, 956
AluBI AGCT 9 cut(s) 320, 558, 587, 596, 759, 1066, 1171, 1211, 1229
AluI AGCT 9 cut(s) 320, 558, 587, 596, 759, 1066, 1171, 1211, 1229
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 2 cut(s) 189, 710
AlwI GGATC 3 cut(s) 378, 391, 420
AlwNI CAGNNNCTG 2 cut(s) 134, 611
Ama87I CYCGRG 1 cut(s) 208
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 2 cut(s) 660, 837
ApeKI GCWGC 6 cut(s) 38, 125, 134, 422, 978, 1058
ApoI RAATTY 1 cut(s) 895
AseI ATTAAT 1 cut(s) 77
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 3 cut(s) 164, 503, 660
AsuNHI GCTAGC 1 cut(s) 748
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 2 cut(s) 164, 503
BamHI GGATCC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 534
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 6 cut(s) 50, 121, 137, 434, 965, 1070
BccI CCATC 2 cut(s) 374, 992
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 2 cut(s) 867, 918
BciVI GTATCC 3 cut(s) 169, 327, 882
BcoDI GTCTC 2 cut(s) 189, 710
BfaI CTAG 4 cut(s) 317, 476, 749, 1230
BfmI CTRYAG 1 cut(s) 420
BfoI RGCGCY 1 cut(s) 464
BfuI GTATCC 3 cut(s) 169, 327, 882
BisI GCNGC 7 cut(s) 39, 94, 126, 135, 423, 979, 1059
BlsI GCNGC 7 cut(s) 40, 95, 127, 136, 424, 980, 1060
Bme1390I CCNGG 2 cut(s) 867, 918
Bme18I GGWCC 2 cut(s) 164, 503
BmeT110I CYCGRG 1 cut(s) 208
BmgT120I GGNCC 3 cut(s) 164, 503, 660
BmiI GGNNCC 4 cut(s) 385, 661, 942, 1012
BmrFI CCNGG 2 cut(s) 867, 918
BmtI GCTAGC 1 cut(s) 752
BpiI GAAGAC 1 cut(s) 534
BpmI CTGGAG 1 cut(s) 1121
Bsa29I ATCGAT 2 cut(s) 369, 415
BsaAI YACGTR 1 cut(s) 955
BsaI GGTCTC 1 cut(s) 189
BsaWI WCCGGW 1 cut(s) 161
Bsc4I CCNNNNNNNGG 4 cut(s) 393, 702, 923, 1046
Bse1I ACTGG 2 cut(s) 408, 1155
Bse3DI GCAATG 2 cut(s) 759, 1127
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 2 cut(s) 867, 918
BseCI ATCGAT 2 cut(s) 369, 415
BseGI GGATG 3 cut(s) 139, 711, 1138
BseLI CCNNNNNNNGG 4 cut(s) 393, 702, 923, 1046
BseMI GCAATG 2 cut(s) 759, 1127
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 2 cut(s) 408, 1155
BseRI GAGGAG 1 cut(s) 116
BseXI GCAGC 6 cut(s) 50, 121, 137, 434, 965, 1070
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 2 cut(s) 662, 839
BshVI ATCGAT 2 cut(s) 369, 415
BsiHKAI GWGCWC 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 2 cut(s) 162, 663
BslI CCNNNNNNNGG 4 cut(s) 393, 702, 923, 1046
BsmAI GTCTC 2 cut(s) 189, 710
BsnI GGCC 2 cut(s) 662, 839
Bso31I GGTCTC 1 cut(s) 189
BsoBI CYCGRG 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 90
Bsp13I TCCGGA 1 cut(s) 161
Bsp143I GATC 3 cut(s) 383, 412, 416
BspACI CCGC 6 cut(s) 51, 69, 93, 96, 456, 734
BspANI GGCC 2 cut(s) 662, 839
BspCNI CTCAG 1 cut(s) 76
BspDI ATCGAT 2 cut(s) 369, 415
BspEI TCCGGA 1 cut(s) 161
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 4 cut(s) 385, 661, 942, 1012
BspMAI CTGCAG 1 cut(s) 424
BspOI GCTAGC 1 cut(s) 752
BspPI GGATC 3 cut(s) 378, 391, 420
BspTNI GGTCTC 1 cut(s) 189
BsrDI GCAATG 2 cut(s) 759, 1127
BsrI ACTGG 2 cut(s) 408, 1155
BssMI GATC 3 cut(s) 383, 412, 416
Bst2UI CCWGG 2 cut(s) 867, 918
Bst4CI ACNGT 3 cut(s) 241, 618, 1166
Bst6I CTCTTC 2 cut(s) 791, 843
BstAPI GCANNNNNTGC 3 cut(s) 134, 428, 788
BstBAI YACGTR 1 cut(s) 955
BstC8I GCNNGC 3 cut(s) 250, 750, 841
BstDEI CTNAG 3 cut(s) 84, 111, 834
BstENI CCTNNNNNAGG 1 cut(s) 921
BstF5I GGATG 3 cut(s) 139, 711, 1138
BstFNI CGCG 1 cut(s) 248
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 3 cut(s) 386, 415, 419
BstMAI GTCTC 2 cut(s) 189, 710
BstMBI GATC 3 cut(s) 383, 412, 416
BstMWI GCNNNNNNNGC 7 cut(s) 131, 134, 428, 593, 668, 788, 836
BstNI CCWGG 2 cut(s) 867, 918
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 2 cut(s) 865, 916
BstSFI CTRYAG 1 cut(s) 420
BstSNI TACGTA 1 cut(s) 955
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 6 cut(s) 50, 121, 137, 434, 965, 1070
BstV2I GAAGAC 1 cut(s) 534
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
Bsu15I ATCGAT 2 cut(s) 369, 415
BsuI GTATCC 3 cut(s) 169, 327, 882
BsuRI GGCC 2 cut(s) 662, 839
BsuTUI ATCGAT 2 cut(s) 369, 415
BtsCI GGATG 3 cut(s) 139, 711, 1138
BtsI GCAGTG 2 cut(s) 832, 850
BtsIMutI CAGTG 2 cut(s) 832, 850
Cac8I GCNNGC 3 cut(s) 250, 750, 841
CaiI CAGNNNCTG 2 cut(s) 134, 611
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 3 cut(s) 164, 503, 660
ClaI ATCGAT 2 cut(s) 369, 415
CseI GACGC 1 cut(s) 1025
Csp6I GTAC 1 cut(s) 440
CviAII CATG 3 cut(s) 155, 311, 995
CviQI GTAC 1 cut(s) 440
DdeI CTNAG 3 cut(s) 84, 111, 834
DpnI GATC 3 cut(s) 385, 414, 418
DpnII GATC 3 cut(s) 383, 412, 416
Eam1104I CTCTTC 2 cut(s) 791, 843
EarI CTCTTC 2 cut(s) 791, 843
EciI GGCGGA 1 cut(s) 111
Eco105I TACGTA 1 cut(s) 955
Eco31I GGTCTC 1 cut(s) 189
Eco32I GATATC 1 cut(s) 108
Eco47I GGWCC 2 cut(s) 164, 503
Eco57I CTGAAG 1 cut(s) 745
Eco88I CYCGRG 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 921
EcoRII CCWGG 2 cut(s) 865, 916
EcoRV GATATC 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 1161
FaeI CATG 3 cut(s) 158, 314, 998
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FatI CATG 3 cut(s) 154, 310, 994
FauI CCCGC 1 cut(s) 741
Fnu4HI GCNGC 7 cut(s) 39, 94, 126, 135, 423, 979, 1059
FokI GGATG 3 cut(s) 126, 718, 1145
Fsp4HI GCNGC 7 cut(s) 39, 94, 126, 135, 423, 979, 1059
FspBI CTAG 4 cut(s) 317, 476, 749, 1230
GlaI GCGC 1 cut(s) 462
GluI GCNGC 7 cut(s) 39, 94, 126, 135, 423, 979, 1059
GsuI CTGGAG 1 cut(s) 1121
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 2 cut(s) 662, 839
HapII CCGG 2 cut(s) 162, 663
HgaI GACGC 1 cut(s) 1025
HhaI GCGC 1 cut(s) 463
Hin1II CATG 3 cut(s) 158, 314, 998
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HindIII AAGCTT 2 cut(s) 594, 1169
HinfI GANTC 2 cut(s) 718, 913
HpaII CCGG 2 cut(s) 162, 663
Hpy188I TCNGA 4 cut(s) 186, 449, 764, 1132
Hpy188III TCNNGA 2 cut(s) 162, 1100
HpyAV CCTTC 9 cut(s) 209, 211, 510, 545, 556, 695, 747, 1001, 1214
HpyCH4III ACNGT 3 cut(s) 241, 618, 1166
HpyCH4IV ACGT 1 cut(s) 954
HpyCH4V TGCA 7 cut(s) 4, 74, 422, 644, 830, 875, 1159
HpyF10VI GCNNNNNNNGC 7 cut(s) 131, 134, 428, 593, 668, 788, 836
HpyF3I CTNAG 3 cut(s) 84, 111, 834
HpySE526I ACGT 1 cut(s) 954
Hsp92II CATG 3 cut(s) 158, 314, 998
HspAI GCGC 1 cut(s) 461
Kpn2I TCCGGA 1 cut(s) 161
Kzo9I GATC 3 cut(s) 383, 412, 416
LmnI GCTCC 6 cut(s) 325, 458, 819, 868, 1010, 1216
Lsp1109I GCAGC 6 cut(s) 50, 121, 137, 434, 965, 1070
MaeI CTAG 4 cut(s) 317, 476, 749, 1230
MaeII ACGT 1 cut(s) 954
MaeIII GTNAC 1 cut(s) 965
MalI GATC 3 cut(s) 385, 414, 418
MboI GATC 3 cut(s) 383, 412, 416
MboII GAAGA 7 cut(s) 41, 44, 539, 808, 860, 892, 922
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 6 cut(s) 172, 187, 226, 350, 883, 895
MlyI GAGTC 1 cut(s) 712
MmeI TCCRAC 4 cut(s) 194, 530, 1064, 1110
Mph1103I ATGCAT 1 cut(s) 1161
MroI TCCGGA 1 cut(s) 161
MseI TTAA 4 cut(s) 77, 342, 365, 804
MspI CCGG 2 cut(s) 162, 663
MspR9I CCNGG 2 cut(s) 867, 918
MvaI CCWGG 2 cut(s) 867, 918
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 7 cut(s) 131, 134, 428, 593, 668, 788, 836
NdeII GATC 3 cut(s) 383, 412, 416
NheI GCTAGC 1 cut(s) 748
NlaIII CATG 3 cut(s) 158, 314, 998
NlaIV GGNNCC 4 cut(s) 385, 661, 942, 1012
NmuCI GTSAC 1 cut(s) 965
NsiI ATGCAT 1 cut(s) 1161
NspI RCATGY 1 cut(s) 314
PaeR7I CTCGAG 1 cut(s) 208
PfeI GAWTC 1 cut(s) 913
PkrI GCNGC 7 cut(s) 40, 95, 127, 136, 424, 980, 1060
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
Ppu21I YACGTR 1 cut(s) 955
PshBI ATTAAT 1 cut(s) 77
PsiI TTATAA 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 865, 916
PspGI CCWGG 2 cut(s) 865, 916
PspN4I GGNNCC 4 cut(s) 385, 661, 942, 1012
PspPI GGNCC 3 cut(s) 164, 503, 660
PspXI VCTCGAGB 1 cut(s) 208
PstI CTGCAG 1 cut(s) 424
PstNI CAGNNNCTG 2 cut(s) 134, 611
PsuI RGATCY 1 cut(s) 383
RsaI GTAC 1 cut(s) 441
RsaNI GTAC 1 cut(s) 440
SaqAI TTAA 4 cut(s) 77, 342, 365, 804
SatI GCNGC 7 cut(s) 39, 94, 126, 135, 423, 979, 1059
Sau3AI GATC 3 cut(s) 383, 412, 416
Sau96I GGNCC 3 cut(s) 164, 503, 660
SchI GAGTC 1 cut(s) 712
ScrFI CCNGG 2 cut(s) 867, 918
SduI GDGCHC 1 cut(s) 90
SfcI CTRYAG 1 cut(s) 420
Sfr274I CTCGAG 1 cut(s) 208
SinI GGWCC 2 cut(s) 164, 503
SlaI CTCGAG 1 cut(s) 208
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
SnaBI TACGTA 1 cut(s) 955
Sse9I AATT 6 cut(s) 172, 187, 226, 350, 883, 895
SsiI CCGC 6 cut(s) 51, 69, 93, 96, 456, 734
SspI AATATT 1 cut(s) 769
SspMI CTAG 4 cut(s) 317, 476, 749, 1230
StyD4I CCNGG 2 cut(s) 865, 916
TaaI ACNGT 3 cut(s) 241, 618, 1166
TaiI ACGT 1 cut(s) 957
TaqI TCGA 5 cut(s) 209, 282, 369, 415, 484
TasI AATT 6 cut(s) 172, 187, 226, 350, 883, 895
TatI WGTACW 1 cut(s) 439
TauI GCSGC 1 cut(s) 96
TfiI GAWTC 1 cut(s) 913
Tru1I TTAA 4 cut(s) 77, 342, 365, 804
Tru9I TTAA 4 cut(s) 77, 342, 365, 804
TscAI CASTG 2 cut(s) 832, 850
TseFI GTSAC 1 cut(s) 965
TseI GCWGC 6 cut(s) 38, 125, 134, 422, 978, 1058
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 3 cut(s) 244, 318, 387
TspGWI ACGGA 1 cut(s) 730
TspRI CASTG 2 cut(s) 832, 850
VpaK11BI GGWCC 2 cut(s) 164, 503
VspI ATTAAT 1 cut(s) 77
XagI CCTNNNNNAGG 1 cut(s) 921
XapI RAATTY 1 cut(s) 895
XceI RCATGY 1 cut(s) 314
XhoI CTCGAG 1 cut(s) 208
XspI CTAG 4 cut(s) 317, 476, 749, 1230
Zsp2I ATGCAT 1 cut(s) 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.