MD17G1136700.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012134
Physical Location & Seq
Forward (+)
12230596 .. 12232741
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1136700.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGCAAACAAACCCAGAACAAAGAGAGAAGGAAGAAGAAGAACGAGGCCGTGCTGTTTCTGGTGCCAGTGCATTAATGACAGAGCACGGCGGCGGCGAGGAGATAGCTATGGAAGTTGTGGCAGCAGAGGTTGCTCATCCATACGCTTTTCATGTATCCGGGCCTCGCAATTTGACCTCTCCGAATTGGAGAGACCTCATAAACTCGAGCTGGAAGGACGGGAATTATAAGCGAACTGTAATCGCGTGCTTCATACAAGCAATATATTTGCTTGAACTCGACAGACAAGAGAATCGAGATGAAGCACATGCTCTAGCTCCTAAATGGTGGACACCCTTTAAGTATAAATTGTCCCAAACCTTAATCGATGAAAGAGATGGATCCACTTTTGGGGCAATACTGGAATGGGATCGATCTGCAGCACTTGCTGACTTTGTACTTATCAGACCAAGCGGAGCACCAAAGGCGGTTCTAGCACTTCGAGGAACATTGCTCAAAGGCCCAACTATGAGAAGGGATATTGAAGACGACCTTCGTTTTCTTGCTTGGGAGAGCTTGAAGGGTTCTGTGAGGTTTAAAACAGCTATGGAGGCTTTGAAATCAGTTGCTGAACGGTATGGAAGCAGTAATGTGTGTATTGCAGGACATTCATTGGGGGCCGGATTTGCTCTCCAAGTGGGAAAAGCATTAGCTAAAGAAGGGATGTATGTGGAGACTCATCTGTTCAATCCACCTTCTGTGTCGCTGGCAATGAGCTTCAGAAATATTGGAGACAAAGCAGGATTTGCTTGGAAGAGATTTAAGGCAATGCTCCCTTCGGGCAGTGGAACTCAGGTCAGCAGTGAAGAGGGGGATAAAGCTCCTGGTTTTGCATTGAAGGATTGGATACCAAATTTTTCGGGTTTGAAGAATCCTGGTGTAGGCTTGGGAAAATGGGTTCCTCATTTATATGTAAATAACAGTGACTATATCTGCTGCTCTTACACCGAACGTGATGGAATGGAAGGAGCCAATGCCGACAAGGAGAATGTGCGTCCAACAAACGGGCAAGTAGCAGCGAAGCTGTTTGTGATGTCAAAAGGAAACCAGAAGTTTCTGGAGGCTCACGGGTTAGAGCAATGGTGGTCGGATGACCTGGAACTTCAGCTGGCTATGCATAACAGTAAGCTCATTAGCAGGCAACTGAAATCCTTGCATAGCGTTCCAGCTCCACAACAAACGCAAGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

411

Amino Acids

45.4

Weight (kDa)

7.16

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50890
fragaria_vesca FvH4_6g38560
malus_domestica MD09G1149800.v1.1 MD17G1136700.v1.1
prunus_persica Prupe.3G176300_v2.0.a1
pyrus_communis pycom09g06920 pycom17g13030
rosa_chinensis RchiOBHm_Chr2g0151971
rosa_laevigata RLG00000020595
rosa_multiflora Rmu_sc0005294.1_g000001 Rmu_sc0023925.1_g000001
rosa_roxburghii Rroxscaffold_2G00096090
rosa_rugosa Rorug02G0430000
rosa_samantha Rh2AG490600 Rh2BG502900 Rh2CG477000 Rh2DG514100
rosa_wichuraiana Rw0G020220 Rw2G040320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 62
AccII CGCG 1 cut(s) 245
AciI CCGC 4 cut(s) 90, 93, 453, 467
AclWI GGATC 3 cut(s) 375, 388, 417
AcsI RAATTY 1 cut(s) 892
AcuI CTGAAG 2 cut(s) 742, 1127
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 3 cut(s) 390, 920, 1043
AgsI TTSAA 7 cut(s) 275, 524, 559, 598, 727, 877, 907
AjnI CCWGG 3 cut(s) 862, 913, 1134
AloI GAACNNNNNNTCC 2 cut(s) 921, 953
Alw21I GWGCWC 2 cut(s) 87, 460
Alw26I GTCTC 3 cut(s) 186, 707, 765
AlwI GGATC 3 cut(s) 375, 388, 417
AlwNI CAGNNNCTG 1 cut(s) 608
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 4 cut(s) 46, 161, 499, 657
ApeKI GCWGC 4 cut(s) 122, 419, 975, 1055
ApoI RAATTY 1 cut(s) 892
AseI ATTAAT 1 cut(s) 74
AspS9I GGNCC 3 cut(s) 161, 500, 657
AsuC2I CCSGG 1 cut(s) 160
AvaI CYCGRG 1 cut(s) 205
BamHI GGATCC 1 cut(s) 380
BanI GGYRCC 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 531
Bbv12I GWGCWC 2 cut(s) 87, 460
BbvI GCAGC 4 cut(s) 134, 431, 962, 1067
BccI CCATC 2 cut(s) 371, 989
BceAI ACGGC 2 cut(s) 33, 103
BciT130I CCWGG 3 cut(s) 864, 915, 1136
BciVI GTATCC 2 cut(s) 166, 879
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 3 cut(s) 186, 707, 765
BfaI CTAG 3 cut(s) 314, 473, 1227
BfmI CTRYAG 1 cut(s) 417
BfuI GTATCC 2 cut(s) 166, 879
BisI GCNGC 6 cut(s) 91, 94, 123, 420, 976, 1056
BlsI GCNGC 6 cut(s) 92, 95, 124, 421, 977, 1057
Bme1390I CCNGG 4 cut(s) 160, 864, 915, 1136
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 3 cut(s) 161, 500, 657
BmiI GGNNCC 5 cut(s) 64, 382, 658, 939, 1009
BmrFI CCNGG 4 cut(s) 160, 864, 915, 1136
BpiI GAAGAC 1 cut(s) 531
BpmI CTGGAG 1 cut(s) 1118
BpuMI CCSGG 1 cut(s) 160
Bsa29I ATCGAT 2 cut(s) 366, 412
BsaI GGTCTC 1 cut(s) 186
Bsc4I CCNNNNNNNGG 3 cut(s) 390, 920, 1043
Bse1I ACTGG 2 cut(s) 66, 405
Bse3DI GCAATG 4 cut(s) 488, 756, 813, 1124
BseBI CCWGG 3 cut(s) 864, 915, 1136
BseCI ATCGAT 2 cut(s) 366, 412
BseGI GGATG 3 cut(s) 136, 708, 1135
BseLI CCNNNNNNNGG 3 cut(s) 390, 920, 1043
BseMI GCAATG 4 cut(s) 488, 756, 813, 1124
BseMII CTCAG 1 cut(s) 845
BseNI ACTGG 2 cut(s) 66, 405
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 4 cut(s) 134, 431, 962, 1067
Bsh1236I CGCG 1 cut(s) 245
BshFI GGCC 4 cut(s) 48, 163, 501, 659
BshNI GGYRCC 1 cut(s) 62
BshVI ATCGAT 2 cut(s) 366, 412
BsiHKAI GWGCWC 2 cut(s) 87, 460
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 2 cut(s) 159, 660
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 3 cut(s) 390, 920, 1043
BsmAI GTCTC 3 cut(s) 186, 707, 765
BsmFI GGGAC 1 cut(s) 337
BsnI GGCC 4 cut(s) 48, 163, 501, 659
Bso31I GGTCTC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 205
Bsp1286I GDGCHC 2 cut(s) 87, 460
Bsp143I GATC 3 cut(s) 380, 409, 413
BspACI CCGC 4 cut(s) 90, 93, 453, 467
BspANI GGCC 4 cut(s) 48, 163, 501, 659
BspCNI CTCAG 1 cut(s) 844
BspDI ATCGAT 2 cut(s) 366, 412
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 5 cut(s) 64, 382, 658, 939, 1009
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 3 cut(s) 375, 388, 417
BspT107I GGYRCC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 186
BsrDI GCAATG 4 cut(s) 488, 756, 813, 1124
BsrI ACTGG 2 cut(s) 66, 405
BssMI GATC 3 cut(s) 380, 409, 413
Bst2UI CCWGG 3 cut(s) 864, 915, 1136
Bst4CI ACNGT 4 cut(s) 238, 615, 962, 1163
Bst6I CTCTTC 2 cut(s) 788, 840
BstAPI GCANNNNNTGC 3 cut(s) 131, 425, 785
BstC8I GCNNGC 5 cut(s) 247, 747, 1149, 1178, 1224
BstDEI CTNAG 1 cut(s) 831
BstENI CCTNNNNNAGG 1 cut(s) 918
BstF5I GGATG 3 cut(s) 136, 708, 1135
BstFNI CGCG 1 cut(s) 245
BstKTI GATC 3 cut(s) 383, 412, 416
BstMAI GTCTC 3 cut(s) 186, 707, 765
BstMBI GATC 3 cut(s) 380, 409, 413
BstMWI GCNNNNNNNGC 8 cut(s) 131, 425, 464, 473, 590, 665, 785, 1153
BstNI CCWGG 3 cut(s) 864, 915, 1136
BstNSI RCATGY 1 cut(s) 311
BstSCI CCNGG 4 cut(s) 158, 862, 913, 1134
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 4 cut(s) 134, 431, 962, 1067
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
Bsu15I ATCGAT 2 cut(s) 366, 412
BsuI GTATCC 2 cut(s) 166, 879
BsuRI GGCC 4 cut(s) 48, 163, 501, 659
BsuTUI ATCGAT 2 cut(s) 366, 412
BtsCI GGATG 3 cut(s) 136, 708, 1135
BtsI GCAGTG 2 cut(s) 829, 847
BtsIMutI CAGTG 4 cut(s) 73, 829, 847, 967
Cac8I GCNNGC 5 cut(s) 247, 747, 1149, 1178, 1224
CaiI CAGNNNCTG 1 cut(s) 608
Cfr13I GGNCC 3 cut(s) 161, 500, 657
ClaI ATCGAT 2 cut(s) 366, 412
CseI GACGC 1 cut(s) 1022
Csp6I GTAC 1 cut(s) 437
CviAII CATG 2 cut(s) 152, 308
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 1 cut(s) 831
DpnI GATC 3 cut(s) 382, 411, 415
DpnII GATC 3 cut(s) 380, 409, 413
DraI TTTAAA 1 cut(s) 577
Eam1104I CTCTTC 2 cut(s) 788, 840
EarI CTCTTC 2 cut(s) 788, 840
Eco31I GGTCTC 1 cut(s) 186
Eco57I CTGAAG 2 cut(s) 742, 1127
Eco88I CYCGRG 1 cut(s) 205
EcoNI CCTNNNNNAGG 1 cut(s) 918
EcoRII CCWGG 3 cut(s) 862, 913, 1134
EcoT22I ATGCAT 1 cut(s) 1158
FaeI CATG 2 cut(s) 155, 311
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 1 cut(s) 337
FatI CATG 2 cut(s) 151, 307
Fnu4HI GCNGC 6 cut(s) 91, 94, 123, 420, 976, 1056
FokI GGATG 3 cut(s) 123, 715, 1142
Fsp4HI GCNGC 6 cut(s) 91, 94, 123, 420, 976, 1056
FspBI CTAG 3 cut(s) 314, 473, 1227
GluI GCNGC 6 cut(s) 91, 94, 123, 420, 976, 1056
GsuI CTGGAG 1 cut(s) 1118
HaeIII GGCC 4 cut(s) 48, 163, 501, 659
HapII CCGG 2 cut(s) 159, 660
HgaI GACGC 1 cut(s) 1022
Hin1II CATG 2 cut(s) 155, 311
HinfI GANTC 3 cut(s) 292, 715, 910
HpaII CCGG 2 cut(s) 159, 660
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 4 cut(s) 183, 446, 761, 1129
Hpy188III TCNNGA 2 cut(s) 296, 1097
Hpy8I GTNNAC 1 cut(s) 330
HpyCH4III ACNGT 4 cut(s) 238, 615, 962, 1163
HpyCH4IV ACGT 1 cut(s) 991
HpyCH4V TGCA 7 cut(s) 4, 71, 419, 641, 872, 1156, 1195
HpyF10VI GCNNNNNNNGC 8 cut(s) 131, 425, 464, 473, 590, 665, 785, 1153
HpyF3I CTNAG 1 cut(s) 831
HpySE526I ACGT 1 cut(s) 991
Hsp92II CATG 2 cut(s) 155, 311
Kzo9I GATC 3 cut(s) 380, 409, 413
LmnI GCTCC 6 cut(s) 322, 455, 816, 865, 1007, 1213
Lsp1109I GCAGC 4 cut(s) 134, 431, 962, 1067
MaeI CTAG 3 cut(s) 314, 473, 1227
MaeII ACGT 1 cut(s) 991
MaeIII GTNAC 1 cut(s) 962
MalI GATC 3 cut(s) 382, 411, 415
MboI GATC 3 cut(s) 380, 409, 413
MboII GAAGA 7 cut(s) 44, 47, 50, 536, 805, 857, 919
MflI RGATCY 1 cut(s) 380
MhlI GDGCHC 2 cut(s) 87, 460
MluCI AATT 5 cut(s) 169, 184, 223, 347, 892
MlyI GAGTC 1 cut(s) 709
MmeI TCCRAC 2 cut(s) 1061, 1107
Mph1103I ATGCAT 1 cut(s) 1158
MseI TTAA 5 cut(s) 74, 339, 362, 576, 801
MslI CAYNNNNRTG 1 cut(s) 948
MspA1I CMGCKG 1 cut(s) 1147
MspI CCGG 2 cut(s) 159, 660
MspR9I CCNGG 4 cut(s) 160, 864, 915, 1136
MvaI CCWGG 3 cut(s) 864, 915, 1136
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 8 cut(s) 131, 425, 464, 473, 590, 665, 785, 1153
NciI CCSGG 1 cut(s) 160
NdeII GATC 3 cut(s) 380, 409, 413
NlaIII CATG 2 cut(s) 155, 311
NlaIV GGNNCC 5 cut(s) 64, 382, 658, 939, 1009
NmuCI GTSAC 1 cut(s) 962
NsiI ATGCAT 1 cut(s) 1158
NspI RCATGY 1 cut(s) 311
PaeR7I CTCGAG 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 2 cut(s) 292, 910
PkrI GCNGC 6 cut(s) 92, 95, 124, 421, 977, 1057
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
PshBI ATTAAT 1 cut(s) 74
PsiI TTATAA 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 862, 913, 1134
PspGI CCWGG 3 cut(s) 862, 913, 1134
PspN4I GGNNCC 5 cut(s) 64, 382, 658, 939, 1009
PspPI GGNCC 3 cut(s) 161, 500, 657
PspXI VCTCGAGB 1 cut(s) 205
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 1 cut(s) 380
PvuII CAGCTG 1 cut(s) 1147
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
RseI CAYNNNNRTG 1 cut(s) 948
SaqAI TTAA 5 cut(s) 74, 339, 362, 576, 801
SatI GCNGC 6 cut(s) 91, 94, 123, 420, 976, 1056
Sau3AI GATC 3 cut(s) 380, 409, 413
Sau96I GGNCC 3 cut(s) 161, 500, 657
SchI GAGTC 1 cut(s) 709
ScrFI CCNGG 4 cut(s) 160, 864, 915, 1136
SduI GDGCHC 2 cut(s) 87, 460
SfcI CTRYAG 1 cut(s) 417
Sfr274I CTCGAG 1 cut(s) 205
SlaI CTCGAG 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
Sse9I AATT 5 cut(s) 169, 184, 223, 347, 892
SsiI CCGC 4 cut(s) 90, 93, 453, 467
SspI AATATT 1 cut(s) 766
SspMI CTAG 3 cut(s) 314, 473, 1227
StyD4I CCNGG 4 cut(s) 158, 862, 913, 1134
TaaI ACNGT 4 cut(s) 238, 615, 962, 1163
TaiI ACGT 1 cut(s) 994
TaqI TCGA 6 cut(s) 206, 279, 295, 366, 412, 481
TasI AATT 5 cut(s) 169, 184, 223, 347, 892
TatI WGTACW 1 cut(s) 436
TauI GCSGC 2 cut(s) 93, 96
TfiI GAWTC 2 cut(s) 292, 910
Tru1I TTAA 5 cut(s) 74, 339, 362, 576, 801
Tru9I TTAA 5 cut(s) 74, 339, 362, 576, 801
TscAI CASTG 4 cut(s) 73, 829, 847, 967
TseFI GTSAC 1 cut(s) 962
TseI GCWGC 4 cut(s) 122, 419, 975, 1055
Tsp45I GTSAC 1 cut(s) 962
TspDTI ATGAA 5 cut(s) 140, 241, 315, 384, 639
TspRI CASTG 4 cut(s) 73, 829, 847, 967
VspI ATTAAT 1 cut(s) 74
XagI CCTNNNNNAGG 1 cut(s) 918
XapI RAATTY 1 cut(s) 892
XceI RCATGY 1 cut(s) 311
XhoI CTCGAG 1 cut(s) 205
XspI CTAG 3 cut(s) 314, 473, 1227
Zsp2I ATGCAT 1 cut(s) 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.