Rh2BG502900

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012134
Physical Location & Seq
Reverse (-)
70903437 .. 70905506
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG502900.1

Sequence Viewer

Length: 1206 bp
ATGCAAGGAGCCCCAAAACAAAACGAAGCTCCAGAAGAAGAAAGAGACGGTGCCGCCTCAGTGTTAATGGAAAAACGCGGTGTCGTTCAGGAAGTTGTGGAAGCGCAGCCTCATCCCTACTCTTTCCACGTCTCGGGTCCTCGGAAATTGTGTTCGCCCAATTGGAGAGACAATATCAGCTCCAGTTGGAAGGATGGGAATTACAAAAGAACTGTCATTGCATGCTTCATACAAGCAATCTACTTACTTGAACTTGATAGACAGGAGAACAGAGATGCAGACAATGCTCTTGCCCCAAAGTGGTGGATACCTTTTAAGTATAAACTAACACAAACTTTAATTGATGAAAGGGATGGATCCATTTTTGGTGCAATTCTGGAGTGGGATCGATCTGCAGCACTTGCGGATTTTGTAGTCATGAGACCCAGTGGGGCACCAAGAGCTGTTTTAGCACTTAGAGGAACATTACTCAAAGGCCCAACGATGCGAAGGGATATCGAAGATGACCTTCGTTTTCTTGCTTGGGAGAGCTTGAAGGGATCTATCAGGTTCAAAGTAGCTCTGGAGGCTCTGAAAATTGTTGCAGAAAGGTATGGAAGCAGTAATGTATGCATTGCAGGCCATTCTTTAGGAGCTGGTTTTGCTCTACAAGTGGGAAAGGCATTAGCTAAAGAAGGGATGTATGTAGAGACTCACTTATTCAATCCACCTTCAGTTTCTCTGGCAATGAGCTTCCGAAATATTGGAGAGAAAGCAGGGTTTGCTTGGAAGAGATTTAAATCAATGCTTCCTATGAGTAGTGAAACTCAGGTCAATAATGAGGAGGACAAGGCTATTGGTTTTGGATTGAAGAACTGGTTATCTGGTTTAAAGGGTCCTCCTGGTGTAGGATTGGGAAAATGGGTTCCTCATCTTTATGTAAATAATAGTGACTACATCTGCTGCTCTTATACTGACCCTGATGGAATAGAAGGAAATAATGCCGGGAAGGAGAATGTGGGTCCAAGTTCAACAACTGGGCAAGTAGCAGCAAAGCTATTTGTGATGTCAAAGGGAAACCAGAAGTTTCTGGAAGCTCATGGATTAGAGCAATGGTGGTCTGATGATCTGGAACTTCAGCTGGCTCTGCATAACAGTAAGCTCATCAGTAGGCAGCTGAAATCCTTGTATAGCCTTCCAGCTCCTCAACCAGCACTTCCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

401

Amino Acids

44.44

Weight (kDa)

8.44

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50890
fragaria_vesca FvH4_6g38560
malus_domestica MD09G1149800.v1.1 MD17G1136700.v1.1
prunus_persica Prupe.3G176300_v2.0.a1
pyrus_communis pycom09g06920 pycom17g13030
rosa_chinensis RchiOBHm_Chr2g0151971
rosa_laevigata RLG00000020595
rosa_multiflora Rmu_sc0005294.1_g000001 Rmu_sc0023925.1_g000001
rosa_roxburghii Rroxscaffold_2G00096090
rosa_rugosa Rorug02G0430000
rosa_samantha Rh2AG490600 Rh2BG502900 Rh2CG477000 Rh2DG514100
rosa_wichuraiana Rw0G020220 Rw2G040320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 50, 433
AccII CGCG 1 cut(s) 78
AciI CCGC 3 cut(s) 54, 78, 404
AclWI GGATC 4 cut(s) 351, 364, 393, 547
AcuI CTGAAG 2 cut(s) 696, 1100
AfiI CCNNNNNNNGG 3 cut(s) 133, 300, 887
AgsI TTSAA 6 cut(s) 251, 535, 553, 703, 850, 1011
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 880
AloI GAACNNNNNNTCC 4 cut(s) 136, 168, 888, 920
Alw26I GTCTC 5 cut(s) 39, 136, 162, 415, 683
AlwI GGATC 4 cut(s) 351, 364, 393, 547
Ama87I CYCGRG 1 cut(s) 133
AoxI GGCC 2 cut(s) 475, 619
ApeKI GCWGC 5 cut(s) 106, 395, 942, 1028, 1153
ArsI GACNNNNNNTTYG 2 cut(s) 198, 230
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 4 cut(s) 137, 476, 875, 1001
AsuC2I CCSGG 1 cut(s) 985
AvaI CYCGRG 1 cut(s) 133
AvaII GGWCC 3 cut(s) 137, 875, 1001
BaeGI GKGCMC 1 cut(s) 436
BamHI GGATCC 1 cut(s) 356
BanI GGYRCC 2 cut(s) 50, 433
BanII GRGCYC 1 cut(s) 13
BbvI GCAGC 5 cut(s) 118, 407, 929, 1040, 1165
BccI CCATC 3 cut(s) 188, 347, 956
BciT130I CCWGG 1 cut(s) 882
BciVI GTATCC 1 cut(s) 300
BcnI CCSGG 1 cut(s) 985
BcoDI GTCTC 5 cut(s) 39, 136, 162, 415, 683
BfaI CTAG 1 cut(s) 1200
BfmI CTRYAG 1 cut(s) 393
BfuI GTATCC 1 cut(s) 300
BisI GCNGC 6 cut(s) 54, 107, 396, 943, 1029, 1154
BlsI GCNGC 6 cut(s) 55, 108, 397, 944, 1030, 1155
Bme1390I CCNGG 2 cut(s) 882, 985
Bme18I GGWCC 3 cut(s) 137, 875, 1001
BmeT110I CYCGRG 1 cut(s) 133
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 4 cut(s) 137, 476, 875, 1001
BmiI GGNNCC 8 cut(s) 10, 52, 138, 358, 435, 876, 906, 1002
BmrFI CCNGG 2 cut(s) 882, 985
BmrI ACTGGG 2 cut(s) 420, 1026
BmsI GCATC 2 cut(s) 265, 474
BmuI ACTGGG 2 cut(s) 420, 1026
BpmI CTGGAG 4 cut(s) 15, 166, 398, 584
BpuMI CCSGG 1 cut(s) 985
Bsa29I ATCGAT 1 cut(s) 388
BsaBI GATNNNNATC 1 cut(s) 778
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 1 cut(s) 140
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 300, 887
Bse1I ACTGG 4 cut(s) 183, 426, 860, 1021
Bse3DI GCAATG 4 cut(s) 216, 612, 732, 1097
Bse8I GATNNNNATC 1 cut(s) 778
BseBI CCWGG 1 cut(s) 882
BseCI ATCGAT 1 cut(s) 388
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 4 cut(s) 112, 199, 358, 684
BseJI GATNNNNATC 1 cut(s) 778
BseLI CCNNNNNNNGG 3 cut(s) 133, 300, 887
BseMI GCAATG 4 cut(s) 216, 612, 732, 1097
BseMII CTCAG 2 cut(s) 72, 821
BseNI ACTGG 4 cut(s) 183, 426, 860, 1021
BseRI GAGGAG 2 cut(s) 836, 1173
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 5 cut(s) 118, 407, 929, 1040, 1165
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 2 cut(s) 477, 621
BshNI GGYRCC 2 cut(s) 50, 433
BshVI ATCGAT 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 133
BsiSI CCGG 1 cut(s) 984
BslI CCNNNNNNNGG 3 cut(s) 133, 300, 887
BsmAI GTCTC 5 cut(s) 39, 136, 162, 415, 683
BsmBI CGTCTC 2 cut(s) 39, 136
BsnI GGCC 2 cut(s) 477, 621
Bso31I GGTCTC 1 cut(s) 415
BsoBI CYCGRG 1 cut(s) 133
Bsp1286I GDGCHC 2 cut(s) 13, 436
Bsp143I GATC 5 cut(s) 356, 385, 389, 539, 1105
BspACI CCGC 3 cut(s) 54, 78, 404
BspANI GGCC 2 cut(s) 477, 621
BspCNI CTCAG 2 cut(s) 71, 820
BspDI ATCGAT 1 cut(s) 388
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 417
BspLI GGNNCC 8 cut(s) 10, 52, 138, 358, 435, 876, 906, 1002
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 4 cut(s) 351, 364, 393, 547
BspT107I GGYRCC 2 cut(s) 50, 433
BspTNI GGTCTC 1 cut(s) 415
BsrDI GCAATG 4 cut(s) 216, 612, 732, 1097
BsrI ACTGG 4 cut(s) 183, 426, 860, 1021
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 5 cut(s) 356, 385, 389, 539, 1105
Bst2UI CCWGG 1 cut(s) 882
Bst4CI ACNGT 3 cut(s) 50, 214, 1136
Bst6I CTCTTC 1 cut(s) 764
BstAPI GCANNNNNTGC 3 cut(s) 284, 401, 761
BstC8I GCNNGC 3 cut(s) 223, 619, 1122
BstDEI CTNAG 3 cut(s) 58, 455, 807
BstENI CCTNNNNNAGG 1 cut(s) 885
BstF5I GGATG 4 cut(s) 112, 199, 358, 684
BstFNI CGCG 1 cut(s) 78
BstHHI GCGC 1 cut(s) 106
BstKTI GATC 5 cut(s) 359, 388, 392, 542, 1108
BstMAI GTCTC 5 cut(s) 39, 136, 162, 415, 683
BstMBI GATC 5 cut(s) 356, 385, 389, 539, 1105
BstMWI GCNNNNNNNGC 9 cut(s) 284, 401, 440, 449, 566, 618, 641, 761, 1126
BstNI CCWGG 1 cut(s) 882
BstNSI RCATGY 1 cut(s) 225
BstSCI CCNGG 2 cut(s) 880, 983
BstSFI CTRYAG 1 cut(s) 393
BstSLI GKGCMC 1 cut(s) 436
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 5 cut(s) 118, 407, 929, 1040, 1165
BstX2I RGATCY 2 cut(s) 356, 539
BstXI CCANNNNNNTGG 1 cut(s) 303
BstYI RGATCY 2 cut(s) 356, 539
Bsu15I ATCGAT 1 cut(s) 388
BsuI GTATCC 1 cut(s) 300
BsuRI GGCC 2 cut(s) 477, 621
BsuTUI ATCGAT 1 cut(s) 388
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 4 cut(s) 112, 199, 358, 684
BtsIMutI CAGTG 2 cut(s) 66, 433
Cac8I GCNNGC 3 cut(s) 223, 619, 1122
CciI TCATGA 1 cut(s) 417
CfoI GCGC 1 cut(s) 106
Cfr13I GGNCC 4 cut(s) 137, 476, 875, 1001
ClaI ATCGAT 1 cut(s) 388
CviAII CATG 3 cut(s) 222, 418, 1079
DdeI CTNAG 3 cut(s) 58, 455, 807
DpnI GATC 5 cut(s) 358, 387, 391, 541, 1107
DpnII GATC 5 cut(s) 356, 385, 389, 539, 1105
DraI TTTAAA 2 cut(s) 778, 870
Eam1104I CTCTTC 1 cut(s) 764
EarI CTCTTC 1 cut(s) 764
Eco24I GRGCYC 1 cut(s) 13
Eco31I GGTCTC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 3 cut(s) 137, 875, 1001
Eco57I CTGAAG 2 cut(s) 696, 1100
Eco88I CYCGRG 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 885
EcoO109I RGGNCCY 2 cut(s) 137, 875
EcoRII CCWGG 1 cut(s) 880
EcoRV GATATC 1 cut(s) 496
EcoT22I ATGCAT 1 cut(s) 614
EcoT38I GRGCYC 1 cut(s) 13
Esp3I CGTCTC 2 cut(s) 39, 136
FaeI CATG 3 cut(s) 225, 421, 1082
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FatI CATG 3 cut(s) 221, 417, 1078
Fnu4HI GCNGC 6 cut(s) 54, 107, 396, 943, 1029, 1154
FokI GGATG 4 cut(s) 99, 206, 365, 691
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 6 cut(s) 54, 107, 396, 943, 1029, 1154
FspBI CTAG 1 cut(s) 1200
GlaI GCGC 1 cut(s) 105
GluI GCNGC 6 cut(s) 54, 107, 396, 943, 1029, 1154
GsuI CTGGAG 4 cut(s) 15, 166, 398, 584
HaeIII GGCC 2 cut(s) 477, 621
HapII CCGG 1 cut(s) 984
HhaI GCGC 1 cut(s) 106
Hin1II CATG 3 cut(s) 225, 421, 1082
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HinfI GANTC 1 cut(s) 691
HpaII CCGG 1 cut(s) 984
Hpy188I TCNGA 4 cut(s) 144, 573, 737, 1102
Hpy188III TCNNGA 7 cut(s) 32, 89, 377, 418, 563, 1070, 1109
HpyAV CCTTC 9 cut(s) 184, 483, 518, 529, 668, 720, 965, 982, 1184
HpyCH4III ACNGT 3 cut(s) 50, 214, 1136
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 9 cut(s) 4, 221, 278, 371, 395, 584, 612, 617, 1129
HpyF10VI GCNNNNNNNGC 9 cut(s) 284, 401, 440, 449, 566, 618, 641, 761, 1126
HpyF3I CTNAG 3 cut(s) 58, 455, 807
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 3 cut(s) 225, 421, 1082
HspAI GCGC 1 cut(s) 104
Kzo9I GATC 5 cut(s) 356, 385, 389, 539, 1105
LmnI GCTCC 5 cut(s) 8, 34, 185, 632, 1186
Lsp1109I GCAGC 5 cut(s) 118, 407, 929, 1040, 1165
LweI GCATC 2 cut(s) 265, 474
MaeI CTAG 1 cut(s) 1200
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 929
MalI GATC 5 cut(s) 358, 387, 391, 541, 1107
MboI GATC 5 cut(s) 356, 385, 389, 539, 1105
MboII GAAGA 5 cut(s) 47, 50, 512, 781, 862
MfeI CAATTG 1 cut(s) 160
MflI RGATCY 2 cut(s) 356, 539
MhlI GDGCHC 2 cut(s) 13, 436
MluCI AATT 6 cut(s) 146, 160, 199, 339, 372, 576
MlyI GAGTC 1 cut(s) 685
MmeI TCCRAC 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 614
MseI TTAA 5 cut(s) 65, 315, 338, 777, 869
MslI CAYNNNNRTG 1 cut(s) 915
MspA1I CMGCKG 2 cut(s) 1120, 1156
MspI CCGG 1 cut(s) 984
MspR9I CCNGG 2 cut(s) 882, 985
MunI CAATTG 1 cut(s) 160
MvaI CCWGG 1 cut(s) 882
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 9 cut(s) 284, 401, 440, 449, 566, 618, 641, 761, 1126
NciI CCSGG 1 cut(s) 985
NdeII GATC 5 cut(s) 356, 385, 389, 539, 1105
NlaIII CATG 3 cut(s) 225, 421, 1082
NlaIV GGNNCC 8 cut(s) 10, 52, 138, 358, 435, 876, 906, 1002
NmuCI GTSAC 1 cut(s) 929
NsiI ATGCAT 1 cut(s) 614
NspI RCATGY 1 cut(s) 225
PaeI GCATGC 1 cut(s) 225
PagI TCATGA 1 cut(s) 417
PkrI GCNGC 6 cut(s) 55, 108, 397, 944, 1030, 1155
PleI GAGTC 1 cut(s) 685
PpsI GAGTC 1 cut(s) 685
PpuMI RGGWCCY 2 cut(s) 137, 875
Psp5II RGGWCCY 2 cut(s) 137, 875
Psp6I CCWGG 1 cut(s) 880
PspGI CCWGG 1 cut(s) 880
PspN4I GGNNCC 8 cut(s) 10, 52, 138, 358, 435, 876, 906, 1002
PspPI GGNCC 4 cut(s) 137, 476, 875, 1001
PspPPI RGGWCCY 2 cut(s) 137, 875
PstI CTGCAG 1 cut(s) 397
PsuI RGATCY 2 cut(s) 356, 539
PvuII CAGCTG 2 cut(s) 1120, 1156
RseI CAYNNNNRTG 1 cut(s) 915
SaqAI TTAA 5 cut(s) 65, 315, 338, 777, 869
SatI GCNGC 6 cut(s) 54, 107, 396, 943, 1029, 1154
Sau3AI GATC 5 cut(s) 356, 385, 389, 539, 1105
Sau96I GGNCC 4 cut(s) 137, 476, 875, 1001
SchI GAGTC 1 cut(s) 685
ScrFI CCNGG 2 cut(s) 882, 985
SduI GDGCHC 2 cut(s) 13, 436
SfaNI GCATC 2 cut(s) 265, 474
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 3 cut(s) 137, 875, 1001
SmiI ATTTAAAT 1 cut(s) 778
SmiMI CAYNNNNRTG 1 cut(s) 915
SphI GCATGC 1 cut(s) 225
Sse9I AATT 6 cut(s) 146, 160, 199, 339, 372, 576
SsiI CCGC 3 cut(s) 54, 78, 404
SspI AATATT 1 cut(s) 742
SspMI CTAG 1 cut(s) 1200
StyD4I CCNGG 2 cut(s) 880, 983
SwaI ATTTAAAT 1 cut(s) 778
TaaI ACNGT 3 cut(s) 50, 214, 1136
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 388, 498
TasI AATT 6 cut(s) 146, 160, 199, 339, 372, 576
TauI GCSGC 1 cut(s) 56
Tru1I TTAA 5 cut(s) 65, 315, 338, 777, 869
Tru9I TTAA 5 cut(s) 65, 315, 338, 777, 869
TscAI CASTG 2 cut(s) 66, 433
TseFI GTSAC 1 cut(s) 929
TseI GCWGC 5 cut(s) 106, 395, 942, 1028, 1153
Tsp45I GTSAC 1 cut(s) 929
TspDTI ATGAA 2 cut(s) 217, 360
TspRI CASTG 2 cut(s) 66, 433
VpaK11BI GGWCC 3 cut(s) 137, 875, 1001
XagI CCTNNNNNAGG 1 cut(s) 885
XceI RCATGY 1 cut(s) 225
XspI CTAG 1 cut(s) 1200
Zsp2I ATGCAT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.