AT5G51690

aminotransferase ACS12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015320
Physical Location & Seq
Reverse (-)
20997413 .. 21001054
3642 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G51690.1

Sequence Viewer

Length: 1488 bp
ATGAGGTTGATAGTACCTCTCCGTGGAGTAATTCAAGGCCGTGGTGGTCTCTTCGTCGGCTCTCTCATTCCTTGTTGTCTCTTCTACTTTCTCCAGCTCTACCTTAAACGACGCCGTCCTCCTCCCTCCGACCCGACGGACTTACCGAGAACTTTTTCACGGACTAATCTCTTCTCTCGCGGGAACTCGATCGGACGAGTTCGAGTCTCCTCCCGAGCAGTTCCTGTAGCTAAACCTTCCGATTCGCCTTATTACATTGGGTTGGAGAGAGTCAAAACGGATCCGTACGATCGGATTACGAACACGGATGGGATTATTCAGCTTGGATTAGCCGAGAGTACGCTGTGCTTTGACTTGCTACAGAGATGGATGTCAGAGAATTTGATGGAGTCAATGATGCAATCTGATGATGGTGAATTTGATATTAGTAGTATCGCCATGTATAAACCTTTTGAAGGATTACTTGAACTCAGAGTGGCTTTTGCTGATTTCATGTCACGAATAATGGGAGGTAATGTGTCATTTGATCCCTCAAATATGGTTATAACAGCCGGTGGGACTCCTGCTATTGAAGTATTGGCCTTCTGTTTGGCTGATCATGGAAATGCTTTTCTAATTCCGACGCCATATTATCCAGGCTTTGATAGGGATATTAAATTCCGGACAGGAGTGGAGCTTATACCAGTACACTGCCGTAGCTCTGACAATTTTACTGTAACGGTTTCCGCACTGGAACAAGCTTTGAATCAGGCTAGAAAACGAGGAAGCAAGGTTTCTGGCATCCTCTTTTCAAACCCGTCAAATCCTGTTGGAAACATACTATCAAGGGAGACGCTTTGTGATATTTTGAGATTTGCTCAAGAGAAGAACATCCATGTTATTTCTGATGAAATATTTGCGGGTTCTGTTTACGGGGACAAAGAGTTTGTTAGCATGGCTGAGATCGCTGGCTCAGGGGAATTTGATAAGACTAGGGTTCATATCATATATGGCTTGTCAAAAGACCTTTCAATTCCTGGTTTTAGAGCTGGAGTCATCTACTCCTTTCATGAAGACGTAGTAAATGCTGCAAAGAAGCTGATGAGATTTTCATCAGTGCCAGTTCTAGTTCAAAGGATACTTATCTCTCTACTATCTGATGTAAGATTCATTGAGGGATACATGGCAGCACACAGGCAAAGGATCCGGGATAAGCATATTCGGTTTGTGGAAGGTTTGAAACAATTAGGAATACCATGTGCTGAGAGTGGTGGTGGGTTGTATTGTTGGGTAGACATGAGCAGTTTACTGACATCTTACAGTGAGAAAGGAGAACTCGAGTTATTTGAGAAGCTATTGACTGTTGCTAAGATTAATGCCACTCCAGGAACAGCCTGTTATTGTATAGAACCAGGTTGGTTCAGGTGCTGTTTCACCGCTTTAGCTGATGAAGATATCCCAGTGATTATGGAACGAATCAGACAGCTTGCTGAATCATTTAGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

495

Amino Acids

55.21

Weight (kDa)

7.03

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 106 - 486 1.6e-81 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51690 AT5G51690
fragaria_vesca FvH4_7g28260
malus_domestica MD01G1186400.v1.1
prunus_persica Prupe.2G283100_v2.0.a1
pyrus_communis pycom01g19800
rosa_chinensis RchiOBHm_Chr1g0375231
rosa_laevigata RLG00000026677
rosa_multiflora Rmu_co8467605.1_g000001
rosa_roxburghii Rroxscaffold_4G00282920
rosa_rugosa Rorug01G0389200
rosa_samantha Rh1AG399900 Rh1BG364500 Rh1CG377500 Rh1DG395100
rosa_wichuraiana Rw1G035500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AccI GTMKAC 1 cut(s) 1272
AccII CGCG 1 cut(s) 180
AccIII TCCGGA 1 cut(s) 660
AciI CCGC 4 cut(s) 180, 726, 899, 1416
AclWI GGATC 5 cut(s) 275, 288, 521, 1177, 1190
AcsI RAATTY 4 cut(s) 379, 416, 656, 959
AcyI GRCGYC 2 cut(s) 112, 623
AfaI GTAC 4 cut(s) 15, 287, 340, 687
AfiI CCNNNNNNNGG 2 cut(s) 23, 455
AgsI TTSAA 9 cut(s) 35, 455, 467, 572, 745, 792, 1011, 1112, 1219
AjnI CCWGG 4 cut(s) 634, 1015, 1363, 1390
Alw26I GTCTC 4 cut(s) 53, 83, 211, 824
AlwI GGATC 5 cut(s) 275, 288, 521, 1177, 1190
AlwNI CAGNNNCTG 2 cut(s) 224, 1407
Ama87I CYCGRG 2 cut(s) 213, 1316
Aor13HI TCCGGA 1 cut(s) 660
AoxI GGCC 2 cut(s) 37, 579
ApeKI GCWGC 2 cut(s) 1067, 1166
ApoI RAATTY 4 cut(s) 379, 416, 656, 959
ArsI GACNNNNNNTTYG 2 cut(s) 908, 940
AseI ATTAAT 1 cut(s) 1353
Asp700I GAANNNNTTC 1 cut(s) 154
AsuC2I CCSGG 1 cut(s) 1187
AsuHPI GGTGA 2 cut(s) 425, 1405
AvaI CYCGRG 2 cut(s) 213, 1316
BamHI GGATCC 2 cut(s) 280, 1182
BbsI GAAGAC 1 cut(s) 1059
BbvI GCAGC 2 cut(s) 1054, 1178
BccI CCATC 4 cut(s) 302, 360, 379, 404
BceAI ACGGC 3 cut(s) 24, 99, 678
BciT130I CCWGG 4 cut(s) 636, 1017, 1365, 1392
BciVI GTATCC 2 cut(s) 1110, 1151
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 1 cut(s) 1187
BcoDI GTCTC 4 cut(s) 53, 83, 211, 824
BfaI CTAG 3 cut(s) 753, 972, 1106
BfmI CTRYAG 2 cut(s) 225, 359
BfuI GTATCC 2 cut(s) 1110, 1151
BglII AGATCT 1 cut(s) 1481
BisI GCNGC 2 cut(s) 1068, 1167
BlsI GCNGC 2 cut(s) 1069, 1168
Bme1390I CCNGG 5 cut(s) 636, 1017, 1187, 1365, 1392
BmeT110I CYCGRG 2 cut(s) 213, 1316
BmiI GGNNCC 2 cut(s) 282, 1184
BmrFI CCNGG 5 cut(s) 636, 1017, 1187, 1365, 1392
BmrI ACTGGG 1 cut(s) 1433
BmsI GCATC 2 cut(s) 387, 789
BmuI ACTGGG 1 cut(s) 1433
BpiI GAAGAC 1 cut(s) 1059
BplI GAGNNNNNCTC 2 cut(s) 841, 873
BpmI CTGGAG 3 cut(s) 77, 1050, 1347
Bpu10I CCTNAGC 1 cut(s) 952
BpuEI CTTGAG 1 cut(s) 843
BpuMI CCSGG 1 cut(s) 1187
BsaBI GATNNNNATC 2 cut(s) 1090, 1121
BsaHI GRCGYC 2 cut(s) 112, 623
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 22, 40
BsaWI WCCGGW 1 cut(s) 660
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 455
Bse118I RCCGGY 1 cut(s) 551
Bse1I ACTGG 4 cut(s) 683, 735, 1100, 1439
Bse8I GATNNNNATC 2 cut(s) 1090, 1121
BseAI TCCGGA 1 cut(s) 660
BseBI CCWGG 4 cut(s) 636, 1017, 1365, 1392
BseDI CCNNGG 2 cut(s) 22, 40
BseGI GGATG 4 cut(s) 313, 375, 780, 870
BseJI GATNNNNATC 2 cut(s) 1090, 1121
BseLI CCNNNNNNNGG 2 cut(s) 23, 455
BseMII CTCAG 4 cut(s) 484, 930, 966, 1233
BseNI ACTGG 4 cut(s) 683, 735, 1100, 1439
BseRI GAGGAG 2 cut(s) 111, 199
BseXI GCAGC 2 cut(s) 1054, 1178
Bsh1236I CGCG 1 cut(s) 180
Bsh1285I CGRYCG 2 cut(s) 192, 292
BshFI GGCC 2 cut(s) 39, 581
BsiEI CGRYCG 2 cut(s) 192, 292
BsiHKCI CYCGRG 2 cut(s) 213, 1316
BsiSI CCGG 3 cut(s) 552, 661, 1186
BsiWI CGTACG 1 cut(s) 285
BslFI GGGAC 2 cut(s) 571, 929
BslI CCNNNNNNNGG 2 cut(s) 23, 455
BsmAI GTCTC 4 cut(s) 53, 83, 211, 824
BsmBI CGTCTC 1 cut(s) 824
BsmFI GGGAC 2 cut(s) 571, 929
BsnI GGCC 2 cut(s) 39, 581
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 2 cut(s) 213, 1316
Bsp13I TCCGGA 1 cut(s) 660
Bsp143I GATC 8 cut(s) 189, 280, 289, 526, 595, 942, 1182, 1481
BspACI CCGC 4 cut(s) 180, 726, 899, 1416
BspANI GGCC 2 cut(s) 39, 581
BspCNI CTCAG 4 cut(s) 483, 931, 965, 1234
BspEI TCCGGA 1 cut(s) 660
BspFNI CGCG 1 cut(s) 180
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 2 cut(s) 282, 1184
BspPI GGATC 5 cut(s) 275, 288, 521, 1177, 1190
BspTNI GGTCTC 1 cut(s) 53
BsrFI RCCGGY 1 cut(s) 551
BsrI ACTGG 4 cut(s) 683, 735, 1100, 1439
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 2 cut(s) 22, 40
BssMI GATC 8 cut(s) 189, 280, 289, 526, 595, 942, 1182, 1481
BssNI GRCGYC 2 cut(s) 112, 623
Bst2UI CCWGG 4 cut(s) 636, 1017, 1365, 1392
Bst4CI ACNGT 4 cut(s) 715, 721, 1301, 1342
Bst6I CTCTTC 3 cut(s) 56, 86, 176
BstACI GRCGYC 2 cut(s) 112, 623
BstC8I GCNNGC 2 cut(s) 949, 1467
BstDEI CTNAG 5 cut(s) 470, 939, 952, 1242, 1347
BstDSI CCRYGG 2 cut(s) 22, 40
BstENI CCTNNNNNAGG 1 cut(s) 453
BstF5I GGATG 4 cut(s) 313, 375, 780, 870
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 8 cut(s) 192, 283, 292, 529, 598, 945, 1185, 1484
BstMAI GTCTC 4 cut(s) 53, 83, 211, 824
BstMBI GATC 8 cut(s) 189, 280, 289, 526, 595, 942, 1182, 1481
BstMCI CGRYCG 2 cut(s) 192, 292
BstMWI GCNNNNNNNGC 1 cut(s) 944
BstNI CCWGG 4 cut(s) 636, 1017, 1365, 1392
BstSCI CCNGG 5 cut(s) 634, 1015, 1185, 1363, 1390
BstSFI CTRYAG 2 cut(s) 225, 359
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 2 cut(s) 1054, 1178
BstV2I GAAGAC 1 cut(s) 1059
BstX2I RGATCY 3 cut(s) 280, 1182, 1481
BstYI RGATCY 3 cut(s) 280, 1182, 1481
BsuI GTATCC 2 cut(s) 1110, 1151
BsuRI GGCC 2 cut(s) 39, 581
BtgI CCRYGG 2 cut(s) 22, 40
BtsCI GGATG 4 cut(s) 313, 375, 780, 870
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 5 cut(s) 688, 728, 1101, 1306, 1446
Cac8I GCNNGC 2 cut(s) 949, 1467
CaiI CAGNNNCTG 2 cut(s) 224, 1407
CciI TCATGA 1 cut(s) 1048
Cfr10I RCCGGY 1 cut(s) 551
CseI GACGC 3 cut(s) 120, 631, 841
CsiI ACCWGGT 1 cut(s) 1390
Csp6I GTAC 4 cut(s) 14, 286, 339, 686
CviAII CATG 9 cut(s) 439, 493, 599, 875, 934, 1049, 1162, 1236, 1276
CviQI GTAC 4 cut(s) 14, 286, 339, 686
DdeI CTNAG 5 cut(s) 470, 939, 952, 1242, 1347
DpnI GATC 8 cut(s) 191, 282, 291, 528, 597, 944, 1184, 1483
DpnII GATC 8 cut(s) 189, 280, 289, 526, 595, 942, 1182, 1481
Eam1104I CTCTTC 3 cut(s) 56, 86, 176
EarI CTCTTC 3 cut(s) 56, 86, 176
Eco31I GGTCTC 1 cut(s) 53
Eco32I GATATC 1 cut(s) 1435
Eco88I CYCGRG 2 cut(s) 213, 1316
EcoNI CCTNNNNNAGG 1 cut(s) 453
EcoRII CCWGG 4 cut(s) 634, 1015, 1363, 1390
EcoRV GATATC 1 cut(s) 1435
Esp3I CGTCTC 1 cut(s) 824
FaeI CATG 9 cut(s) 442, 496, 602, 878, 937, 1052, 1165, 1239, 1279
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FaqI GGGAC 2 cut(s) 571, 929
FatI CATG 9 cut(s) 438, 492, 598, 874, 933, 1048, 1161, 1235, 1275
FauI CCCGC 2 cut(s) 173, 892
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 1272
Fnu4HI GCNGC 2 cut(s) 1068, 1167
FokI GGATG 4 cut(s) 320, 382, 767, 857
Fsp4HI GCNGC 2 cut(s) 1068, 1167
FspBI CTAG 3 cut(s) 753, 972, 1106
GluI GCNGC 2 cut(s) 1068, 1167
GsuI CTGGAG 3 cut(s) 77, 1050, 1347
HaeIII GGCC 2 cut(s) 39, 581
HapII CCGG 3 cut(s) 552, 661, 1186
HgaI GACGC 3 cut(s) 120, 631, 841
Hin1I GRCGYC 2 cut(s) 112, 623
Hin1II CATG 9 cut(s) 442, 496, 602, 878, 937, 1052, 1165, 1239, 1279
HindIII AAGCTT 1 cut(s) 738
HpaII CCGG 3 cut(s) 552, 661, 1186
HphI GGTGA 2 cut(s) 425, 1405
Hpy166II GTNNAC 4 cut(s) 688, 910, 1273, 1286
Hpy188III TCNNGA 6 cut(s) 213, 498, 661, 860, 1049, 1485
Hpy8I GTNNAC 4 cut(s) 688, 910, 1273, 1286
Hpy99I CGWCG 4 cut(s) 59, 114, 139, 625
HpyAV CCTTC 4 cut(s) 246, 449, 592, 1205
HpyCH4III ACNGT 4 cut(s) 715, 721, 1301, 1342
HpyCH4IV ACGT 1 cut(s) 1056
HpyCH4V TGCA 2 cut(s) 400, 1070
HpyF10VI GCNNNNNNNGC 1 cut(s) 944
HpyF3I CTNAG 5 cut(s) 470, 939, 952, 1242, 1347
HpySE526I ACGT 1 cut(s) 1056
Hsp92I GRCGYC 2 cut(s) 112, 623
Hsp92II CATG 9 cut(s) 442, 496, 602, 878, 937, 1052, 1165, 1239, 1279
Kpn2I TCCGGA 1 cut(s) 660
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 8 cut(s) 189, 280, 289, 526, 595, 942, 1182, 1481
LmnI GCTCC 1 cut(s) 673
Lsp1109I GCAGC 2 cut(s) 1054, 1178
LweI GCATC 2 cut(s) 387, 789
MabI ACCWGGT 1 cut(s) 1390
MaeI CTAG 3 cut(s) 753, 972, 1106
MaeII ACGT 1 cut(s) 1056
MaeIII GTNAC 2 cut(s) 495, 715
MalI GATC 8 cut(s) 191, 282, 291, 528, 597, 944, 1184, 1483
MboI GATC 8 cut(s) 189, 280, 289, 526, 595, 942, 1182, 1481
MboII GAAGA 6 cut(s) 43, 73, 163, 877, 1064, 1442
MflI RGATCY 3 cut(s) 280, 1182, 1481
MluCI AATT 9 cut(s) 30, 379, 416, 615, 656, 706, 959, 1011, 1223
MlyI GAGTC 5 cut(s) 213, 279, 398, 553, 1041
MmeI TCCRAC 4 cut(s) 153, 243, 644, 790
MroI TCCGGA 1 cut(s) 660
MroXI GAANNNNTTC 1 cut(s) 154
MseI TTAA 3 cut(s) 105, 654, 1353
MslI CAYNNNNRTG 1 cut(s) 603
MspI CCGG 3 cut(s) 552, 661, 1186
MspR9I CCNGG 5 cut(s) 636, 1017, 1187, 1365, 1392
MvaI CCWGG 4 cut(s) 636, 1017, 1365, 1392
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 1 cut(s) 944
NciI CCSGG 1 cut(s) 1187
NdeII GATC 8 cut(s) 189, 280, 289, 526, 595, 942, 1182, 1481
NlaIII CATG 9 cut(s) 442, 496, 602, 878, 937, 1052, 1165, 1239, 1279
NlaIV GGNNCC 2 cut(s) 282, 1184
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 1 cut(s) 495
PaeR7I CTCGAG 1 cut(s) 1316
PagI TCATGA 1 cut(s) 1048
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 154
PfeI GAWTC 5 cut(s) 242, 745, 1146, 1455, 1472
Pfl23II CGTACG 1 cut(s) 285
PflFI GACNNNGTC 1 cut(s) 114
PfoI TCCNGGA 2 cut(s) 1185, 1363
PkrI GCNGC 2 cut(s) 1069, 1168
Ple19I CGATCG 2 cut(s) 192, 292
PleI GAGTC 5 cut(s) 212, 278, 397, 553, 1040
PpsI GAGTC 5 cut(s) 212, 278, 397, 553, 1040
PshBI ATTAAT 1 cut(s) 1353
PsiI TTATAA 1 cut(s) 545
Psp6I CCWGG 4 cut(s) 634, 1015, 1363, 1390
PspGI CCWGG 4 cut(s) 634, 1015, 1363, 1390
PspLI CGTACG 1 cut(s) 285
PspN4I GGNNCC 2 cut(s) 282, 1184
PspXI VCTCGAGB 1 cut(s) 1316
PstNI CAGNNNCTG 2 cut(s) 224, 1407
PsuI RGATCY 3 cut(s) 280, 1182, 1481
PsyI GACNNNGTC 1 cut(s) 114
PvuI CGATCG 2 cut(s) 192, 292
RsaI GTAC 4 cut(s) 15, 287, 340, 687
RsaNI GTAC 4 cut(s) 14, 286, 339, 686
RseI CAYNNNNRTG 1 cut(s) 603
SaqAI TTAA 3 cut(s) 105, 654, 1353
SatI GCNGC 2 cut(s) 1068, 1167
Sau3AI GATC 8 cut(s) 189, 280, 289, 526, 595, 942, 1182, 1481
SchI GAGTC 5 cut(s) 213, 279, 398, 553, 1041
ScrFI CCNGG 5 cut(s) 636, 1017, 1187, 1365, 1392
SexAI ACCWGGT 1 cut(s) 1390
SfaNI GCATC 2 cut(s) 387, 789
SfcI CTRYAG 2 cut(s) 225, 359
Sfr274I CTCGAG 1 cut(s) 1316
SlaI CTCGAG 1 cut(s) 1316
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 2 cut(s) 858, 1316
SmoI CTYRAG 2 cut(s) 858, 1316
Sse9I AATT 9 cut(s) 30, 379, 416, 615, 656, 706, 959, 1011, 1223
SsiI CCGC 4 cut(s) 180, 726, 899, 1416
SspI AATATT 1 cut(s) 894
SspMI CTAG 3 cut(s) 753, 972, 1106
StyD4I CCNGG 5 cut(s) 634, 1015, 1185, 1363, 1390
TaaI ACNGT 4 cut(s) 715, 721, 1301, 1342
TaiI ACGT 1 cut(s) 1059
TaqI TCGA 3 cut(s) 188, 202, 1317
TasI AATT 9 cut(s) 30, 379, 416, 615, 656, 706, 959, 1011, 1223
TatI WGTACW 1 cut(s) 685
TfiI GAWTC 5 cut(s) 242, 745, 1146, 1455, 1472
Tru1I TTAA 3 cut(s) 105, 654, 1353
Tru9I TTAA 3 cut(s) 105, 654, 1353
TscAI CASTG 5 cut(s) 695, 735, 1101, 1306, 1446
TseFI GTSAC 1 cut(s) 495
TseI GCWGC 2 cut(s) 1067, 1166
Tsp45I GTSAC 1 cut(s) 495
TspDTI ATGAA 8 cut(s) 481, 903, 968, 1037, 1065, 1080, 1138, 1443
TspGWI ACGGA 6 cut(s) 11, 152, 175, 273, 293, 320
TspRI CASTG 5 cut(s) 695, 735, 1101, 1306, 1446
Tth111I GACNNNGTC 1 cut(s) 114
VspI ATTAAT 1 cut(s) 1353
XagI CCTNNNNNAGG 1 cut(s) 453
XapI RAATTY 4 cut(s) 379, 416, 656, 959
XhoI CTCGAG 1 cut(s) 1316
XmiI GTMKAC 1 cut(s) 1272
XmnI GAANNNNTTC 1 cut(s) 154
XspI CTAG 3 cut(s) 753, 972, 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.