MD01G1186400.v1.1

aminotransferase ACS12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015320
Physical Location & Seq
Reverse (-)
28510462 .. 28514771
4310 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1186400.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGAGACTGATTGTGCCACTCCAAGGCGTCGTCCAAGGCAGAGGCGGCCTCATCCTCGGCTCTCTAATCCCATGTGCTCTCTATTACTGCCTCCAGCTCTACCTAAAACGACGCCGACCCTCCAAACCCGATCCACCTTCCCCCTCCCAGTCCTCCTCCAACCTGGCGGAGCTCCCCAGAACGCTGTCGCGGTCCAGCTTGTCCACCCGTGGGTCCATGGGCCGGGTCCGGATGTCCTCCCGGGCGAGCTGGGTTGCGAAGCCCAATGACTCGCCGTACTATATCGGGTTGGATAGGGCTTCGGAGGACCCGTACGATAGGGTGGATAATCCGGATGGGGTTATTCAACTTGGATTGTCCGAGAACAGGCTGTGTTTGGATTTGATTGAGAAATGGGTTTCGGAGAATCTGATGAAATCGATGGTGGGAACGGACGGTGGTGATTTGAGCATAATTGGGATTGCAGCGTACCAGCCGTTTGATGGAATGACTGAGCTGAAAGTGGCTATGGCGAGTTTCATGTCTCAGATCATGGGAAGATCAGTGTCATTTGACCCATCGCAAATAGTGTTAACATCTGGTGCAACCCCTGCAGTTGAGATATTAAGCTTCTGTTTAGCAGACCATGGAAATGCATTTCTCGTTCCTACACCTTATTATCCCGGTTTTGACAGGGATATGAGATGGCGAACAGGAGTGGAGCTGATTCCCGTTCACTGTCGCAGCACTGACAACTTCACTTTGAATATAACTGTTCTCGATCAAGCATACAGTCAAGCTAGAAAACGAGGAGTAAAAGTGCAGGGAGTTCTCATATCTAACCCTTCAAATCCTGTGGGTAATTTGCTTTCAAGGGAAATGCTGTGCAGTCTTCTAGACTTTGCCCAAGAGAAGAACATCCATATCATCTCTGATGAAATATTTGCCGGGTCTATGTATGGAAGTGAGGAGTTCGTGAGCATGGCAGAAATTGTTGACTCAGAAGATTTTGAGAAGAGCAGGGTTCACATAATATATGGTCTATCGAAAGACTTATCTATTCCGGGATTCAGGGTTGGGGTTTTATATTCATATAATGAGAGTGTGTTGGCAGCAGCTAAAAAGTTAACAAGATTTTCTCCCATTTCTTCTCTGACCCAGCGGCTTATTATTTCAATGCTCTCAGATACAAGATTCATTCAGGAATACCTACAGATCAACAAAAAGCGGATAAAAGATTTGTACGATTTATTTGTGGCTGGTTTACAACAATTAGGAATCAAGTGTGCAAAGAGCAGTGCTGGTTTATACTGTTGGGCTGATATGAGTGGATTAATTCGCTCTTATGGTGAGAAGGGGGAACTCGAGTTATGGGATAAGCTGTTGAACATTGCCAAGATCAATGTGACTCCTGGTTCAGCCTGCCATTGCATAGAACCAGGATGGTTCAGATGTTGTTTTACTACTTTGACTCCAGAGGATGTTCCTGTAGTTATAGACCGGATCCAGAAAGTTACTGAAACAATTAAGTCTTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

507

Amino Acids

56.09

Weight (kDa)

7.46

Isoelectric Point (pI)

53.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 115 - 495 6.1e-84 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51690 AT5G51690
fragaria_vesca FvH4_7g28260
malus_domestica MD01G1186400.v1.1
prunus_persica Prupe.2G283100_v2.0.a1
pyrus_communis pycom01g19800
rosa_chinensis RchiOBHm_Chr1g0375231
rosa_laevigata RLG00000026677
rosa_multiflora Rmu_co8467605.1_g000001
rosa_roxburghii Rroxscaffold_4G00282920
rosa_rugosa Rorug01G0389200
rosa_samantha Rh1AG399900 Rh1BG364500 Rh1CG377500 Rh1DG395100
rosa_wichuraiana Rw1G035500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 190
AccIII TCCGGA 2 cut(s) 228, 331
AciI CCGC 5 cut(s) 45, 167, 190, 1141, 1207
AclWI GGATC 3 cut(s) 125, 1477, 1490
AcyI GRCGYC 2 cut(s) 27, 112
AfaI GTAC 4 cut(s) 278, 314, 470, 1223
AfiI CCNNNNNNNGG 5 cut(s) 23, 210, 222, 366, 482
AgsI TTSAA 6 cut(s) 347, 745, 828, 852, 1155, 1366
AjnI CCWGG 3 cut(s) 162, 1390, 1417
Alw21I GWGCWC 2 cut(s) 79, 174
Alw26I GTCTC 1 cut(s) 528
AlwI GGATC 3 cut(s) 125, 1477, 1490
Ama87I CYCGRG 2 cut(s) 240, 1343
Aor13HI TCCGGA 2 cut(s) 228, 331
AoxI GGCC 2 cut(s) 46, 220
ApeKI GCWGC 4 cut(s) 464, 723, 1091, 1094
AseI ATTAAT 1 cut(s) 1313
AspS9I GGNCC 5 cut(s) 192, 213, 220, 226, 307
AsuC2I CCSGG 6 cut(s) 224, 241, 242, 663, 928, 1044
AsuHPI GGTGA 2 cut(s) 452, 1340
AvaI CYCGRG 2 cut(s) 240, 1343
AvaII GGWCC 4 cut(s) 192, 213, 226, 307
BamHI GGATCC 1 cut(s) 1482
BanII GRGCYC 1 cut(s) 174
BbsI GAAGAC 2 cut(s) 863, 1503
Bbv12I GWGCWC 2 cut(s) 79, 174
BbvI GCAGC 4 cut(s) 476, 735, 1103, 1106
BccI CCATC 6 cut(s) 329, 415, 476, 565, 678, 1416
BceAI ACGGC 2 cut(s) 259, 460
BciT130I CCWGG 3 cut(s) 164, 1392, 1419
BcnI CCSGG 6 cut(s) 224, 241, 242, 663, 928, 1044
BcoDI GTCTC 1 cut(s) 528
BfaI CTAG 3 cut(s) 780, 875, 1515
BfmI CTRYAG 3 cut(s) 591, 1190, 1467
BisI GCNGC 6 cut(s) 46, 465, 724, 1092, 1095, 1142
BlsI GCNGC 6 cut(s) 47, 466, 725, 1093, 1096, 1143
Bme1390I CCNGG 9 cut(s) 164, 224, 241, 242, 663, 928, 1044, 1392, 1419
Bme18I GGWCC 4 cut(s) 192, 213, 226, 307
BmeT110I CYCGRG 2 cut(s) 240, 1343
BmgT120I GGNCC 5 cut(s) 192, 213, 220, 226, 307
BmiI GGNNCC 4 cut(s) 214, 227, 309, 1484
BmrFI CCNGG 9 cut(s) 164, 224, 241, 242, 663, 928, 1044, 1392, 1419
BmrI ACTGGG 1 cut(s) 142
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 2 cut(s) 863, 1503
BplI GAGNNNNNCTC 2 cut(s) 33, 65
BpmI CTGGAG 2 cut(s) 77, 1437
BpuMI CCSGG 6 cut(s) 224, 241, 242, 663, 928, 1044
Bsa29I ATCGAT 1 cut(s) 419
BsaHI GRCGYC 2 cut(s) 27, 112
BsaJI CCNNGG 7 cut(s) 22, 34, 55, 208, 216, 240, 625
BsaWI WCCGGW 3 cut(s) 228, 331, 1479
BsaXI ACNNNNNCTCC 6 cut(s) 296, 326, 783, 813, 1435, 1465
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 210, 222, 366, 482
Bse1I ACTGG 1 cut(s) 148
Bse3DI GCAATG 2 cut(s) 1368, 1405
BseAI TCCGGA 2 cut(s) 228, 331
BseBI CCWGG 3 cut(s) 164, 1392, 1419
BseCI ATCGAT 1 cut(s) 419
BseDI CCNNGG 7 cut(s) 22, 34, 55, 208, 216, 240, 625
BseGI GGATG 6 cut(s) 51, 237, 340, 897, 1427, 1465
BseLI CCNNNNNNNGG 5 cut(s) 23, 210, 222, 366, 482
BseMI GCAATG 2 cut(s) 1368, 1405
BseMII CTCAG 4 cut(s) 483, 539, 993, 1176
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 3 cut(s) 145, 804, 962
BseXI GCAGC 4 cut(s) 476, 735, 1103, 1106
BseYI CCCAGC 2 cut(s) 249, 1137
BsgI GTGCAG 2 cut(s) 821, 886
Bsh1236I CGCG 1 cut(s) 190
BshFI GGCC 2 cut(s) 48, 222
BshVI ATCGAT 1 cut(s) 419
BsiHKAI GWGCWC 2 cut(s) 79, 174
BsiHKCI CYCGRG 2 cut(s) 240, 1343
BsiSI CCGG 8 cut(s) 223, 229, 241, 332, 663, 927, 1043, 1480
BsiWI CGTACG 1 cut(s) 312
BslI CCNNNNNNNGG 5 cut(s) 23, 210, 222, 366, 482
BsmAI GTCTC 1 cut(s) 528
BsnI GGCC 2 cut(s) 48, 222
BsoBI CYCGRG 2 cut(s) 240, 1343
Bsp1286I GDGCHC 2 cut(s) 79, 174
Bsp13I TCCGGA 2 cut(s) 228, 331
Bsp143I GATC 7 cut(s) 130, 528, 539, 760, 1194, 1377, 1482
Bsp19I CCATGG 2 cut(s) 216, 625
BspACI CCGC 5 cut(s) 45, 167, 190, 1141, 1207
BspANI GGCC 2 cut(s) 48, 222
BspCNI CTCAG 4 cut(s) 484, 538, 992, 1175
BspDI ATCGAT 1 cut(s) 419
BspEI TCCGGA 2 cut(s) 228, 331
BspFNI CGCG 1 cut(s) 190
BspLI GGNNCC 4 cut(s) 214, 227, 309, 1484
BspMAI CTGCAG 1 cut(s) 595
BspPI GGATC 3 cut(s) 125, 1477, 1490
BspQI GCTCTTC 1 cut(s) 989
BsrDI GCAATG 2 cut(s) 1368, 1405
BsrI ACTGG 1 cut(s) 148
BssECI CCNNGG 7 cut(s) 22, 34, 55, 208, 216, 240, 625
BssMI GATC 7 cut(s) 130, 528, 539, 760, 1194, 1377, 1482
BssNI GRCGYC 2 cut(s) 27, 112
BssT1I CCWWGG 4 cut(s) 22, 34, 216, 625
Bst2UI CCWGG 3 cut(s) 164, 1392, 1419
Bst4CI ACNGT 5 cut(s) 437, 719, 754, 773, 1292
Bst6I CTCTTC 1 cut(s) 989
BstACI GRCGYC 2 cut(s) 27, 112
BstAPI GCANNNNNTGC 1 cut(s) 590
BstC8I GCNNGC 2 cut(s) 247, 1402
BstDEI CTNAG 4 cut(s) 492, 525, 979, 1162
BstDSI CCRYGG 3 cut(s) 208, 216, 625
BstF5I GGATG 6 cut(s) 51, 237, 340, 897, 1427, 1465
BstFNI CGCG 1 cut(s) 190
BstKTI GATC 7 cut(s) 133, 531, 542, 763, 1197, 1380, 1485
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 7 cut(s) 130, 528, 539, 760, 1194, 1377, 1482
BstMWI GCNNNNNNNGC 2 cut(s) 45, 590
BstNI CCWGG 3 cut(s) 164, 1392, 1419
BstSCI CCNGG 9 cut(s) 162, 222, 239, 240, 661, 926, 1042, 1390, 1417
BstSFI CTRYAG 3 cut(s) 591, 1190, 1467
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 4 cut(s) 476, 735, 1103, 1106
BstV2I GAAGAC 2 cut(s) 863, 1503
BstX2I RGATCY 1 cut(s) 1482
BstYI RGATCY 1 cut(s) 1482
Bsu15I ATCGAT 1 cut(s) 419
BsuRI GGCC 2 cut(s) 48, 222
BsuTUI ATCGAT 1 cut(s) 419
BtgI CCRYGG 3 cut(s) 208, 216, 625
BtgZI GCGATG 1 cut(s) 543
BtsCI GGATG 6 cut(s) 51, 237, 340, 897, 1427, 1465
BtsI GCAGTG 1 cut(s) 1282
BtsIMutI CAGTG 4 cut(s) 549, 715, 726, 1282
Cac8I GCNNGC 2 cut(s) 247, 1402
Cfr13I GGNCC 5 cut(s) 192, 213, 220, 226, 307
Cfr9I CCCGGG 1 cut(s) 240
ClaI ATCGAT 1 cut(s) 419
CseI GACGC 2 cut(s) 16, 120
Csp6I GTAC 4 cut(s) 277, 313, 469, 1222
CspCI CAANNNNNGTGG 2 cut(s) 816, 851
CviAII CATG 6 cut(s) 72, 217, 520, 532, 626, 961
CviQI GTAC 4 cut(s) 277, 313, 469, 1222
DdeI CTNAG 4 cut(s) 492, 525, 979, 1162
DpnI GATC 7 cut(s) 132, 530, 541, 762, 1196, 1379, 1484
DpnII GATC 7 cut(s) 130, 528, 539, 760, 1194, 1377, 1482
Eam1104I CTCTTC 1 cut(s) 989
EarI CTCTTC 1 cut(s) 989
EciI GGCGGA 1 cut(s) 182
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 4 cut(s) 22, 34, 216, 625
Eco24I GRGCYC 1 cut(s) 174
Eco47I GGWCC 4 cut(s) 192, 213, 226, 307
Eco53kI GAGCTC 1 cut(s) 172
Eco88I CYCGRG 2 cut(s) 240, 1343
EcoICRI GAGCTC 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 307
EcoRII CCWGG 3 cut(s) 162, 1390, 1417
EcoT14I CCWWGG 4 cut(s) 22, 34, 216, 625
EcoT22I ATGCAT 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 4 cut(s) 22, 34, 216, 625
FaeI CATG 6 cut(s) 75, 220, 523, 535, 629, 964
FatI CATG 6 cut(s) 71, 216, 519, 531, 625, 960
Fnu4HI GCNGC 6 cut(s) 46, 465, 724, 1092, 1095, 1142
FokI GGATG 6 cut(s) 38, 244, 347, 884, 1434, 1472
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 6 cut(s) 46, 465, 724, 1092, 1095, 1142
FspBI CTAG 3 cut(s) 780, 875, 1515
GluI GCNGC 6 cut(s) 46, 465, 724, 1092, 1095, 1142
GsaI CCCAGC 2 cut(s) 253, 1141
GsuI CTGGAG 2 cut(s) 77, 1437
HaeIII GGCC 2 cut(s) 48, 222
HapII CCGG 8 cut(s) 223, 229, 241, 332, 663, 927, 1043, 1480
HgaI GACGC 2 cut(s) 16, 120
Hin1I GRCGYC 2 cut(s) 27, 112
Hin1II CATG 6 cut(s) 75, 220, 523, 535, 629, 964
HincII GTYRAC 3 cut(s) 573, 976, 1107
HindII GTYRAC 3 cut(s) 573, 976, 1107
HindIII AAGCTT 1 cut(s) 607
HinfI GANTC 9 cut(s) 269, 406, 706, 977, 1047, 1173, 1257, 1387, 1450
HpaI GTTAAC 2 cut(s) 573, 1107
HpaII CCGG 8 cut(s) 223, 229, 241, 332, 663, 927, 1043, 1480
HphI GGTGA 2 cut(s) 452, 1340
Hpy166II GTNNAC 7 cut(s) 204, 573, 715, 976, 1006, 1107, 1244
Hpy188III TCNNGA 8 cut(s) 229, 332, 758, 875, 955, 1181, 1454, 1486
Hpy8I GTNNAC 7 cut(s) 204, 573, 715, 976, 1006, 1107, 1244
Hpy99I CGWCG 2 cut(s) 32, 114
HpyAV CCTTC 3 cut(s) 147, 834, 1327
HpyCH4III ACNGT 5 cut(s) 437, 719, 754, 773, 1292
HpyCH4V TGCA 8 cut(s) 464, 584, 593, 635, 802, 867, 1268, 1410
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 590
HpyF3I CTNAG 4 cut(s) 492, 525, 979, 1162
Hsp92I GRCGYC 2 cut(s) 27, 112
Hsp92II CATG 6 cut(s) 75, 220, 523, 535, 629, 964
Kpn2I TCCGGA 2 cut(s) 228, 331
KspAI GTTAAC 2 cut(s) 573, 1107
Kzo9I GATC 7 cut(s) 130, 528, 539, 760, 1194, 1377, 1482
LguI GCTCTTC 1 cut(s) 989
LmnI GCTCC 3 cut(s) 169, 177, 700
Lsp1109I GCAGC 4 cut(s) 476, 735, 1103, 1106
MaeI CTAG 3 cut(s) 780, 875, 1515
MaeIII GTNAC 2 cut(s) 1384, 1492
MalI GATC 7 cut(s) 132, 530, 541, 762, 1196, 1379, 1484
MboI GATC 7 cut(s) 130, 528, 539, 760, 1194, 1377, 1482
MboII GAAGA 7 cut(s) 549, 863, 904, 995, 1006, 1119, 1503
MflI RGATCY 1 cut(s) 1482
MhlI GDGCHC 2 cut(s) 79, 174
MluCI AATT 6 cut(s) 453, 841, 969, 1250, 1314, 1503
MlyI GAGTC 4 cut(s) 263, 971, 1381, 1444
MmeI TCCRAC 2 cut(s) 183, 270
Mph1103I ATGCAT 1 cut(s) 637
MroI TCCGGA 2 cut(s) 228, 331
MseI TTAA 5 cut(s) 572, 605, 1106, 1313, 1506
MslI CAYNNNNRTG 1 cut(s) 630
MspA1I CMGCKG 1 cut(s) 1141
MspI CCGG 8 cut(s) 223, 229, 241, 332, 663, 927, 1043, 1480
MspR9I CCNGG 9 cut(s) 164, 224, 241, 242, 663, 928, 1044, 1392, 1419
MvaI CCWGG 3 cut(s) 164, 1392, 1419
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 2 cut(s) 45, 590
NciI CCSGG 6 cut(s) 224, 241, 242, 663, 928, 1044
NcoI CCATGG 2 cut(s) 216, 625
NdeII GATC 7 cut(s) 130, 528, 539, 760, 1194, 1377, 1482
NlaIII CATG 6 cut(s) 75, 220, 523, 535, 629, 964
NlaIV GGNNCC 4 cut(s) 214, 227, 309, 1484
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 1384
NsiI ATGCAT 1 cut(s) 637
PaeR7I CTCGAG 1 cut(s) 1343
PciSI GCTCTTC 1 cut(s) 989
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PfeI GAWTC 5 cut(s) 406, 706, 1047, 1173, 1257
Pfl23II CGTACG 1 cut(s) 312
PfoI TCCNGGA 1 cut(s) 1042
PkrI GCNGC 6 cut(s) 47, 466, 725, 1093, 1096, 1143
PleI GAGTC 4 cut(s) 263, 971, 1381, 1444
PpsI GAGTC 4 cut(s) 263, 971, 1381, 1444
PpuMI RGGWCCY 1 cut(s) 307
PshBI ATTAAT 1 cut(s) 1313
Psp124BI GAGCTC 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 3 cut(s) 162, 1390, 1417
PspFI CCCAGC 2 cut(s) 249, 1137
PspGI CCWGG 3 cut(s) 162, 1390, 1417
PspLI CGTACG 1 cut(s) 312
PspN4I GGNNCC 4 cut(s) 214, 227, 309, 1484
PspPI GGNCC 5 cut(s) 192, 213, 220, 226, 307
PspPPI RGGWCCY 1 cut(s) 307
PspXI VCTCGAGB 1 cut(s) 1343
PstI CTGCAG 1 cut(s) 595
PsuI RGATCY 1 cut(s) 1482
RsaI GTAC 4 cut(s) 278, 314, 470, 1223
RsaNI GTAC 4 cut(s) 277, 313, 469, 1222
RseI CAYNNNNRTG 1 cut(s) 630
SacI GAGCTC 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 989
SaqAI TTAA 5 cut(s) 572, 605, 1106, 1313, 1506
SatI GCNGC 6 cut(s) 46, 465, 724, 1092, 1095, 1142
Sau3AI GATC 7 cut(s) 130, 528, 539, 760, 1194, 1377, 1482
Sau96I GGNCC 5 cut(s) 192, 213, 220, 226, 307
SchI GAGTC 4 cut(s) 263, 971, 1381, 1444
ScrFI CCNGG 9 cut(s) 164, 224, 241, 242, 663, 928, 1044, 1392, 1419
SduI GDGCHC 2 cut(s) 79, 174
SfcI CTRYAG 3 cut(s) 591, 1190, 1467
Sfr274I CTCGAG 1 cut(s) 1343
SinI GGWCC 4 cut(s) 192, 213, 226, 307
SlaI CTCGAG 1 cut(s) 1343
SmaI CCCGGG 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 1 cut(s) 1343
SmoI CTYRAG 1 cut(s) 1343
Sse9I AATT 6 cut(s) 453, 841, 969, 1250, 1314, 1503
SsiI CCGC 5 cut(s) 45, 167, 190, 1141, 1207
SspI AATATT 1 cut(s) 921
SspMI CTAG 3 cut(s) 780, 875, 1515
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 9 cut(s) 162, 222, 239, 240, 661, 926, 1042, 1390, 1417
StyI CCWWGG 4 cut(s) 22, 34, 216, 625
TaaI ACNGT 5 cut(s) 437, 719, 754, 773, 1292
TaqI TCGA 4 cut(s) 419, 759, 1025, 1344
TasI AATT 6 cut(s) 453, 841, 969, 1250, 1314, 1503
TauI GCSGC 2 cut(s) 48, 1144
TfiI GAWTC 5 cut(s) 406, 706, 1047, 1173, 1257
Tru1I TTAA 5 cut(s) 572, 605, 1106, 1313, 1506
Tru9I TTAA 5 cut(s) 572, 605, 1106, 1313, 1506
TscAI CASTG 4 cut(s) 549, 722, 733, 1282
TseFI GTSAC 1 cut(s) 1384
TseI GCWGC 4 cut(s) 464, 723, 1091, 1094
Tsp45I GTSAC 1 cut(s) 1384
TspDTI ATGAA 5 cut(s) 428, 508, 930, 1059, 1165
TspGWI ACGGA 1 cut(s) 446
TspMI CCCGGG 1 cut(s) 240
TspRI CASTG 4 cut(s) 549, 722, 733, 1282
VpaK11BI GGWCC 4 cut(s) 192, 213, 226, 307
VspI ATTAAT 1 cut(s) 1313
XbaI TCTAGA 1 cut(s) 874
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XhoI CTCGAG 1 cut(s) 1343
XmaI CCCGGG 1 cut(s) 240
XspI CTAG 3 cut(s) 780, 875, 1515
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.