Rorug01G0389200

aminotransferase ACS12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015320
Physical Location & Seq
Forward (+)
50035969 .. 50040449
4481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0389200.1

Sequence Viewer

Length: 1848 bp
ATGCACACATTTCTGACCCCATTGTCCAAAACTCTATTCCTCACAGAAACCAACCAACAAAACTCACATTTCCCAGCACCTCTTCAGTTTCCGTTACCGGCCATTTTCCGTTGCACTTGGCCGGCATGCAAGCTCTCTGCACCTCTCACAGCTTGGTTCCGGTCATCAGCATTTCCTTTAGAACCTCAAGCTCATCACTTGCATGTACCCCAAAAACCAAATGCTTCCCAAAAAAAATATAGGAAATATACGAACTTTAATGTGGCTACTGATGCAAAGAAGTTTGAGGAAGTGTCAAAGTATGGAAATTTGGTTCCCCTTTACAAGTGCATTTTTTCGGACCAGCTCACGCCGGTTCTTGCGTATCGATGCTTGGTGAAGGAAGATGATAGAGAGGCTCCGAGTTTTCTGTTTGAGTCGGTTGATCAGAGCACCCAGGGGCGATATAGTGTTGTTGGAGCTCAACCAGCAATGGAGATTGTGGCAAAAGAAAATAAGGTGACGATAATGGATCATGAAGGAGGGCGTTCAGTAGAGGAGTTTGTTGAGGATCCAATGGATATTCCGAAAAGAATCTCACAGGGTTGGAGTCCCCAACTTATTCATGAACTTCCAGATGCATTTTGTGGTGGTTGGGTAGGCTTCTTTTCATATGATACAGTTCGTTATGTGGAAAAGAGAAGACTGCCATTCTCAAAGGCACCCAAGGATGACAGAAATCTTCCAGACATGCATTTAGGACTCTATGATGATGTTATAGTGTTCGACCATGTGGAGAAGAAAATTTATGCGATTCACTGGGTGAGGTTAGACCGGTACTCCTCAGTTGAGAAAGCCTATACAGATGGAGCAAAACGGTTGGATGAATTAGTGGCTCGAGTACATAACATTGACTTACCAAGGCTGTCTTCCGGTGCTGTGAGCCTGCATACTCACCATTTTGGTCCTCCTTTAAAGAATTCAAACATGACCAGTGAGGCATACAAGGACATAGTTAAGAAGGCAAAGGAGCATATACTCTCAGGGGATATTTTCCAAATCGTATTAAGTCAGCGATTTGAACGTCGAACATTTGCTGACCCATTTGAAGTCTATAGAGCCCTGAGGACTGTAAATCCGAGTCCATATTTGACTTACTTGCAGGCTAGAGGGTGTATACTGGTTGCTTCAAGTCCAGAAATTCTTATGAGTGCAAAGAAGAAAAAGATTGTGAATCGGCCATTAGCTGGAACTTCTAGAAGAGGGAAGACAATTGAAGAGGATAAAATGTTAGAAGTGCAGCTACTTCATGATGAAAAGCAATGTGCAGAACACATCATGCTGGTTGATTTGGGTCGGAATGATGTTGGAAAGGTCTCCAAATTTGGTTCTGTAAAGGTGGAAAGACTAATGAACGTTGAACGATATTCCCATGTCATGCACATAAGCTCTACGGTCACCGGCGAGCTGCAAGACGGTCTCACTTGCTGGGACGCTCTGCGAGCTGCATTGCCTGTTGGAACTGTCAGTGGTGCACCAAAGGTGAAGGCCATGGAGTTAATCGATCAGTTGGAGGTAAACAGGCGTGGCCCATACAGCGGCGGATTTGGAGCAGTATCCTTTTCCGGTGACATGGACATTGCACTAGCTCTGAGGACCATGGTGATTCCAACAGCAGCCCGTTTTGACACAATGTACTCTTACAACAATGGCAACACCCGCCGGGAGTGGGTTGCATATCTTCAAGCTGGTGCTGGTATAGTTGCTGACAGTGATCCTGATGATGAAAACCAAGAGTGCCAGAACAAAGCTGCTGGTCTTGCTCGTGCCATCGACTTGGCTGAGGCCGCATTTGTTCATGAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

615

Amino Acids

69.32

Weight (kDa)

7.31

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anth_synt_I_N PF04715 114 - 263 4.1e-30 Anthranilate synthase component I, N terminal region
Chorismate_bind PF00425 325 - 596 1.1e-79 chorismate binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51690 AT5G51690
fragaria_vesca FvH4_7g28260
malus_domestica MD01G1186400.v1.1
prunus_persica Prupe.2G283100_v2.0.a1
pyrus_communis pycom01g19800
rosa_chinensis RchiOBHm_Chr1g0375231
rosa_laevigata RLG00000026677
rosa_multiflora Rmu_co8467605.1_g000001
rosa_roxburghii Rroxscaffold_4G00282920
rosa_rugosa Rorug01G0389200
rosa_samantha Rh1AG399900 Rh1BG364500 Rh1CG377500 Rh1DG395100
rosa_wichuraiana Rw1G035500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 700
AccB7I CCANNNNNTGG 1 cut(s) 1226
AccI GTMKAC 1 cut(s) 1156
AciI CCGC 4 cut(s) 1578, 1581, 1699, 1827
AclI AACGTT 1 cut(s) 1395
AclWI GGATC 4 cut(s) 519, 545, 558, 1748
AcoI YGGCCR 3 cut(s) 99, 119, 1217
AcsI RAATTY 5 cut(s) 307, 783, 958, 1179, 1361
AcuI CTGAAG 1 cut(s) 68
AdeI CACNNNGTG 1 cut(s) 802
AfaI GTAC 4 cut(s) 207, 818, 882, 1676
AfiI CCNNNNNNNGG 3 cut(s) 1226, 1577, 1708
AgeI ACCGGT 1 cut(s) 813
AgsI TTSAA 7 cut(s) 963, 1061, 1088, 1170, 1256, 1400, 1724
AjnI CCWGG 1 cut(s) 435
AjuI GAANNNNNNNTTGG 4 cut(s) 20, 52, 892, 924
AloI GAACNNNNNNTCC 2 cut(s) 297, 329
Alw21I GWGCWC 3 cut(s) 434, 463, 1516
Alw26I GTCTC 2 cut(s) 1360, 1463
Alw44I GTGCAC 1 cut(s) 1512
AlwI GGATC 4 cut(s) 519, 545, 558, 1748
Ama87I CYCGRG 1 cut(s) 876
AoxI GGCC 6 cut(s) 99, 119, 1217, 1527, 1567, 1824
ApaLI GTGCAC 1 cut(s) 1512
ApeKI GCWGC 5 cut(s) 1279, 1447, 1484, 1655, 1790
ApoI RAATTY 5 cut(s) 307, 783, 958, 1179, 1361
AsiGI ACCGGT 1 cut(s) 813
AspS9I GGNCC 4 cut(s) 340, 944, 1568, 1635
AsuC2I CCSGG 1 cut(s) 1703
AsuHPI GGTGA 8 cut(s) 388, 511, 814, 926, 1429, 1534, 1619, 1654
AvaI CYCGRG 1 cut(s) 876
AvaII GGWCC 3 cut(s) 340, 944, 1635
AxyI CCTNAGG 1 cut(s) 1103
BaeGI GKGCMC 1 cut(s) 1516
BamHI GGATCC 1 cut(s) 550
BanI GGYRCC 1 cut(s) 700
BanII GRGCYC 2 cut(s) 463, 1102
BarI GAAGNNNNNNTAC 2 cut(s) 1266, 1298
BauI CACGAG 1 cut(s) 1803
BbsI GAAGAC 3 cut(s) 688, 900, 1253
Bbv12I GWGCWC 3 cut(s) 434, 463, 1516
BbvCI CCTCAGC 1 cut(s) 1821
BbvI GCAGC 5 cut(s) 1291, 1434, 1471, 1667, 1777
BccI CCATC 2 cut(s) 839, 1817
BciT130I CCWGG 1 cut(s) 437
BciVI GTATCC 1 cut(s) 1606
BclI TGATCA 1 cut(s) 424
BcnI CCSGG 1 cut(s) 1703
BcoDI GTCTC 2 cut(s) 1360, 1463
BfaI CTAG 3 cut(s) 1146, 1236, 1625
BfmI CTRYAG 1 cut(s) 1093
BfuI GTATCC 1 cut(s) 1606
BisI GCNGC 7 cut(s) 1280, 1448, 1485, 1579, 1656, 1791, 1827
BlsI GCNGC 7 cut(s) 1281, 1449, 1486, 1580, 1657, 1792, 1828
Bme1390I CCNGG 2 cut(s) 437, 1703
Bme18I GGWCC 3 cut(s) 340, 944, 1635
BmeT110I CYCGRG 1 cut(s) 876
BmgT120I GGNCC 4 cut(s) 340, 944, 1568, 1635
BmiI GGNNCC 5 cut(s) 158, 315, 399, 552, 702
BmrFI CCNGG 2 cut(s) 437, 1703
BmrI ACTGGG 1 cut(s) 808
BmsI GCATC 3 cut(s) 262, 359, 607
BmuI ACTGGG 1 cut(s) 808
BpiI GAAGAC 3 cut(s) 688, 900, 1253
Bpu10I CCTNAGC 1 cut(s) 1821
BpuEI CTTGAG 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 1703
Bsa29I ATCGAT 2 cut(s) 367, 1542
BsaI GGTCTC 2 cut(s) 1360, 1463
BsaJI CCNNGG 6 cut(s) 435, 436, 705, 899, 1530, 1638
BsaWI WCCGGW 4 cut(s) 159, 813, 911, 1604
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 3 cut(s) 1226, 1577, 1708
Bse118I RCCGGY 5 cut(s) 97, 121, 352, 813, 1439
Bse1I ACTGG 3 cut(s) 803, 972, 1164
Bse21I CCTNAGG 1 cut(s) 1103
Bse3DI GCAATG 4 cut(s) 477, 1307, 1487, 1617
BseBI CCWGG 1 cut(s) 437
BseCI ATCGAT 2 cut(s) 367, 1542
BseDI CCNNGG 6 cut(s) 435, 436, 705, 899, 1530, 1638
BseGI GGATG 2 cut(s) 715, 868
BseLI CCNNNNNNNGG 3 cut(s) 1226, 1577, 1708
BseMI GCAATG 4 cut(s) 477, 1307, 1487, 1617
BseMII CTCAG 5 cut(s) 837, 1035, 1094, 1622, 1812
BseNI ACTGG 3 cut(s) 803, 972, 1164
BseRI GAGGAG 2 cut(s) 551, 811
BseSI GKGCMC 1 cut(s) 1516
BseXI GCAGC 5 cut(s) 1291, 1434, 1471, 1667, 1777
BseYI CCCAGC 2 cut(s) 73, 1467
BsgI GTGCAG 3 cut(s) 123, 1298, 1326
BshFI GGCC 6 cut(s) 101, 121, 1219, 1529, 1569, 1826
BshNI GGYRCC 1 cut(s) 700
BshTI ACCGGT 1 cut(s) 813
BshVI ATCGAT 2 cut(s) 367, 1542
BsiHKAI GWGCWC 3 cut(s) 434, 463, 1516
BsiHKCI CYCGRG 1 cut(s) 876
BsiSI CCGG 9 cut(s) 98, 122, 160, 353, 814, 912, 1440, 1605, 1702
BslFI GGGAC 2 cut(s) 576, 1484
BslI CCNNNNNNNGG 3 cut(s) 1226, 1577, 1708
BsmAI GTCTC 2 cut(s) 1360, 1463
BsmFI GGGAC 2 cut(s) 576, 1484
BsnI GGCC 6 cut(s) 101, 121, 1219, 1529, 1569, 1826
Bso31I GGTCTC 2 cut(s) 1360, 1463
BsoBI CYCGRG 1 cut(s) 876
Bsp1286I GDGCHC 4 cut(s) 434, 463, 1102, 1516
Bsp143I GATC 5 cut(s) 424, 511, 550, 1543, 1753
Bsp19I CCATGG 2 cut(s) 1530, 1638
BspACI CCGC 4 cut(s) 1578, 1581, 1699, 1827
BspANI GGCC 6 cut(s) 101, 121, 1219, 1529, 1569, 1826
BspCNI CTCAG 5 cut(s) 836, 1034, 1095, 1623, 1813
BspDI ATCGAT 2 cut(s) 367, 1542
BspHI TCATGA 4 cut(s) 514, 604, 1288, 1837
BspLI GGNNCC 5 cut(s) 158, 315, 399, 552, 702
BspPI GGATC 4 cut(s) 519, 545, 558, 1748
BspT107I GGYRCC 1 cut(s) 700
BspTNI GGTCTC 2 cut(s) 1360, 1463
BsrDI GCAATG 4 cut(s) 477, 1307, 1487, 1617
BsrFI RCCGGY 5 cut(s) 97, 121, 352, 813, 1439
BsrI ACTGG 3 cut(s) 803, 972, 1164
BssAI RCCGGY 5 cut(s) 97, 121, 352, 813, 1439
BssECI CCNNGG 6 cut(s) 435, 436, 705, 899, 1530, 1638
BssMI GATC 5 cut(s) 424, 511, 550, 1543, 1753
BssNAI GTATAC 1 cut(s) 1157
BssSI CACGAG 1 cut(s) 1803
BssT1I CCWWGG 4 cut(s) 705, 899, 1530, 1638
Bst1107I GTATAC 1 cut(s) 1157
Bst2BI CACGAG 1 cut(s) 1803
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 7 cut(s) 661, 858, 1111, 1435, 1457, 1504, 1751
Bst6I CTCTTC 3 cut(s) 87, 1234, 1251
BstC8I GCNNGC 7 cut(s) 123, 127, 131, 926, 1143, 1445, 1482
BstDEI CTNAG 5 cut(s) 823, 1021, 1103, 1631, 1821
BstDSI CCRYGG 2 cut(s) 1530, 1638
BstEII GGTNACC 1 cut(s) 1435
BstF5I GGATG 2 cut(s) 715, 868
BstKTI GATC 5 cut(s) 427, 514, 553, 1546, 1756
BstMAI GTCTC 2 cut(s) 1360, 1463
BstMBI GATC 5 cut(s) 424, 511, 550, 1543, 1753
BstMWI GCNNNNNNNGC 7 cut(s) 272, 467, 1481, 1575, 1698, 1799, 1826
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 3 cut(s) 129, 206, 733
BstPI GGTNACC 1 cut(s) 1435
BstSCI CCNGG 2 cut(s) 435, 1701
BstSFI CTRYAG 1 cut(s) 1093
BstSLI GKGCMC 1 cut(s) 1516
BstV1I GCAGC 5 cut(s) 1291, 1434, 1471, 1667, 1777
BstV2I GAAGAC 3 cut(s) 688, 900, 1253
BstX2I RGATCY 1 cut(s) 550
BstXI CCANNNNNNTGG 1 cut(s) 1816
BstYI RGATCY 1 cut(s) 550
BstZ17I GTATAC 1 cut(s) 1157
Bsu15I ATCGAT 2 cut(s) 367, 1542
Bsu36I CCTNAGG 1 cut(s) 1103
BsuI GTATCC 1 cut(s) 1606
BsuRI GGCC 6 cut(s) 101, 121, 1219, 1529, 1569, 1826
BsuTUI ATCGAT 2 cut(s) 367, 1542
BtgI CCRYGG 2 cut(s) 1530, 1638
BtsCI GGATG 2 cut(s) 715, 868
BtsIMutI CAGTG 4 cut(s) 796, 979, 1513, 1756
Cac8I GCNNGC 7 cut(s) 123, 127, 131, 926, 1143, 1445, 1482
CciI TCATGA 4 cut(s) 514, 604, 1288, 1837
Cfr10I RCCGGY 5 cut(s) 97, 121, 352, 813, 1439
Cfr13I GGNCC 4 cut(s) 340, 944, 1568, 1635
ClaI ATCGAT 2 cut(s) 367, 1542
CseI GACGC 1 cut(s) 1481
Csp6I GTAC 4 cut(s) 206, 817, 881, 1675
CspAI ACCGGT 1 cut(s) 813
CviQI GTAC 4 cut(s) 206, 817, 881, 1675
DdeI CTNAG 5 cut(s) 823, 1021, 1103, 1631, 1821
DpnI GATC 5 cut(s) 426, 513, 552, 1545, 1755
DpnII GATC 5 cut(s) 424, 511, 550, 1543, 1753
DraI TTTAAA 1 cut(s) 954
DraIII CACNNNGTG 1 cut(s) 802
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 3 cut(s) 99, 119, 1217
Eam1104I CTCTTC 3 cut(s) 87, 1234, 1251
EarI CTCTTC 3 cut(s) 87, 1234, 1251
EciI GGCGGA 1 cut(s) 1596
Ecl136II GAGCTC 1 cut(s) 461
Eco130I CCWWGG 4 cut(s) 705, 899, 1530, 1638
Eco24I GRGCYC 2 cut(s) 463, 1102
Eco31I GGTCTC 2 cut(s) 1360, 1463
Eco47I GGWCC 3 cut(s) 340, 944, 1635
Eco53kI GAGCTC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 68
Eco81I CCTNAGG 1 cut(s) 1103
Eco88I CYCGRG 1 cut(s) 876
Eco91I GGTNACC 1 cut(s) 1435
EcoICRI GAGCTC 1 cut(s) 461
EcoO65I GGTNACC 1 cut(s) 1435
EcoRI GAATTC 1 cut(s) 958
EcoRII CCWGG 1 cut(s) 435
EcoT14I CCWWGG 4 cut(s) 705, 899, 1530, 1638
EcoT22I ATGCAT 2 cut(s) 622, 735
EcoT38I GRGCYC 2 cut(s) 463, 1102
ErhI CCWWGG 4 cut(s) 705, 899, 1530, 1638
FalI AAGNNNNNCTT 2 cut(s) 892, 924
FaqI GGGAC 2 cut(s) 576, 1484
FauI CCCGC 1 cut(s) 1706
FauNDI CATATG 1 cut(s) 652
FbaI TGATCA 1 cut(s) 424
FblI GTMKAC 1 cut(s) 1156
Fnu4HI GCNGC 7 cut(s) 1280, 1448, 1485, 1579, 1656, 1791, 1827
FokI GGATG 2 cut(s) 722, 875
FriOI GRGCYC 2 cut(s) 463, 1102
Fsp4HI GCNGC 7 cut(s) 1280, 1448, 1485, 1579, 1656, 1791, 1827
FspBI CTAG 3 cut(s) 1146, 1236, 1625
GluI GCNGC 7 cut(s) 1280, 1448, 1485, 1579, 1656, 1791, 1827
GsaI CCCAGC 2 cut(s) 77, 1471
HaeIII GGCC 6 cut(s) 101, 121, 1219, 1529, 1569, 1826
HapII CCGG 9 cut(s) 98, 122, 160, 353, 814, 912, 1440, 1605, 1702
HgaI GACGC 1 cut(s) 1481
HinfI GANTC 8 cut(s) 416, 573, 589, 741, 793, 1120, 1213, 1645
HpaII CCGG 9 cut(s) 98, 122, 160, 353, 814, 912, 1440, 1605, 1702
HphI GGTGA 8 cut(s) 388, 511, 814, 926, 1429, 1534, 1619, 1654
Hpy166II GTNNAC 3 cut(s) 1157, 1514, 1558
Hpy188I TCNGA 8 cut(s) 15, 340, 402, 429, 567, 1119, 1338, 1632
Hpy188III TCNNGA 9 cut(s) 515, 605, 614, 725, 1175, 1236, 1289, 1757, 1838
Hpy8I GTNNAC 3 cut(s) 1157, 1514, 1558
Hpy99I CGWCG 1 cut(s) 1068
HpyAV CCTTC 4 cut(s) 373, 512, 994, 1519
HpyCH4III ACNGT 7 cut(s) 661, 858, 1111, 1435, 1457, 1504, 1751
HpyCH4IV ACGT 2 cut(s) 1063, 1395
HpyF10VI GCNNNNNNNGC 7 cut(s) 272, 467, 1481, 1575, 1698, 1799, 1826
HpyF3I CTNAG 5 cut(s) 823, 1021, 1103, 1631, 1821
HpySE526I ACGT 2 cut(s) 1063, 1395
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 5 cut(s) 424, 511, 550, 1543, 1753
LmnI GCTCC 5 cut(s) 403, 458, 848, 1009, 1589
Lsp1109I GCAGC 5 cut(s) 1291, 1434, 1471, 1667, 1777
LweI GCATC 3 cut(s) 262, 359, 607
MaeI CTAG 3 cut(s) 1146, 1236, 1625
MaeII ACGT 2 cut(s) 1063, 1395
MaeIII GTNAC 4 cut(s) 93, 499, 1435, 1607
MalI GATC 5 cut(s) 426, 513, 552, 1545, 1755
MboI GATC 5 cut(s) 424, 511, 550, 1543, 1753
MfeI CAATTG 1 cut(s) 1251
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 4 cut(s) 434, 463, 1102, 1516
MluCI AATT 7 cut(s) 307, 783, 866, 958, 1179, 1251, 1361
MlyI GAGTC 4 cut(s) 425, 598, 735, 1129
MmeI TCCRAC 8 cut(s) 436, 566, 840, 1316, 1327, 1477, 1530, 1673
Mph1103I ATGCAT 2 cut(s) 622, 735
MroNI GCCGGC 1 cut(s) 121
MseI TTAA 5 cut(s) 258, 953, 996, 1046, 1538
MslI CAYNNNNRTG 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 1578
MspI CCGG 9 cut(s) 98, 122, 160, 353, 814, 912, 1440, 1605, 1702
MspR9I CCNGG 2 cut(s) 437, 1703
MunI CAATTG 1 cut(s) 1251
MvaI CCWGG 1 cut(s) 437
MwoI GCNNNNNNNGC 7 cut(s) 272, 467, 1481, 1575, 1698, 1799, 1826
NaeI GCCGGC 1 cut(s) 123
NciI CCSGG 1 cut(s) 1703
NcoI CCATGG 2 cut(s) 1530, 1638
NdeI CATATG 1 cut(s) 652
NdeII GATC 5 cut(s) 424, 511, 550, 1543, 1753
NgoMIV GCCGGC 1 cut(s) 121
NlaIV GGNNCC 5 cut(s) 158, 315, 399, 552, 702
NmuCI GTSAC 3 cut(s) 499, 1435, 1607
NsiI ATGCAT 2 cut(s) 622, 735
NspI RCATGY 3 cut(s) 129, 206, 733
PaeI GCATGC 1 cut(s) 129
PaeR7I CTCGAG 1 cut(s) 876
PagI TCATGA 4 cut(s) 514, 604, 1288, 1837
PasI CCCWGGG 1 cut(s) 436
PdiI GCCGGC 1 cut(s) 123
PfeI GAWTC 4 cut(s) 573, 793, 1213, 1645
PflMI CCANNNNNTGG 1 cut(s) 1226
PinAI ACCGGT 1 cut(s) 813
PkrI GCNGC 7 cut(s) 1281, 1449, 1486, 1580, 1657, 1792, 1828
PleI GAGTC 4 cut(s) 424, 597, 735, 1128
PpsI GAGTC 4 cut(s) 424, 597, 735, 1128
Psp124BI GAGCTC 1 cut(s) 463
Psp1406I AACGTT 1 cut(s) 1395
Psp6I CCWGG 1 cut(s) 435
PspEI GGTNACC 1 cut(s) 1435
PspFI CCCAGC 2 cut(s) 73, 1467
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 5 cut(s) 158, 315, 399, 552, 702
PspPI GGNCC 4 cut(s) 340, 944, 1568, 1635
PspXI VCTCGAGB 1 cut(s) 876
PsuI RGATCY 1 cut(s) 550
RsaI GTAC 4 cut(s) 207, 818, 882, 1676
RsaNI GTAC 4 cut(s) 206, 817, 881, 1675
RseI CAYNNNNRTG 1 cut(s) 201
SacI GAGCTC 1 cut(s) 463
SaqAI TTAA 5 cut(s) 258, 953, 996, 1046, 1538
SatI GCNGC 7 cut(s) 1280, 1448, 1485, 1579, 1656, 1791, 1827
Sau3AI GATC 5 cut(s) 424, 511, 550, 1543, 1753
Sau96I GGNCC 4 cut(s) 340, 944, 1568, 1635
SchI GAGTC 4 cut(s) 425, 598, 735, 1129
ScrFI CCNGG 2 cut(s) 437, 1703
SduI GDGCHC 4 cut(s) 434, 463, 1102, 1516
SfaNI GCATC 3 cut(s) 262, 359, 607
SfcI CTRYAG 1 cut(s) 1093
Sfr274I CTCGAG 1 cut(s) 876
SgrAI CRCCGGYG 1 cut(s) 1439
SinI GGWCC 3 cut(s) 340, 944, 1635
SlaI CTCGAG 1 cut(s) 876
SmiMI CAYNNNNRTG 1 cut(s) 201
SmlI CTYRAG 2 cut(s) 186, 876
SmoI CTYRAG 2 cut(s) 186, 876
SphI GCATGC 1 cut(s) 129
Sse9I AATT 7 cut(s) 307, 783, 866, 958, 1179, 1251, 1361
SsiI CCGC 4 cut(s) 1578, 1581, 1699, 1827
SspMI CTAG 3 cut(s) 1146, 1236, 1625
SstI GAGCTC 1 cut(s) 463
StyD4I CCNGG 2 cut(s) 435, 1701
StyI CCWWGG 4 cut(s) 705, 899, 1530, 1638
TaaI ACNGT 7 cut(s) 661, 858, 1111, 1435, 1457, 1504, 1751
TaiI ACGT 2 cut(s) 1066, 1398
TaqI TCGA 6 cut(s) 367, 765, 877, 1066, 1542, 1812
TasI AATT 7 cut(s) 307, 783, 866, 958, 1179, 1251, 1361
TatI WGTACW 2 cut(s) 880, 1674
TauI GCSGC 2 cut(s) 1581, 1829
TfiI GAWTC 4 cut(s) 573, 793, 1213, 1645
Tru1I TTAA 5 cut(s) 258, 953, 996, 1046, 1538
Tru9I TTAA 5 cut(s) 258, 953, 996, 1046, 1538
TscAI CASTG 4 cut(s) 803, 979, 1513, 1756
TseFI GTSAC 3 cut(s) 499, 1435, 1607
TseI GCWGC 5 cut(s) 1279, 1447, 1484, 1655, 1790
Tsp45I GTSAC 3 cut(s) 499, 1435, 1607
TspGWI ACGGA 2 cut(s) 81, 98
TspRI CASTG 4 cut(s) 803, 979, 1513, 1756
Van91I CCANNNNNTGG 1 cut(s) 1226
VneI GTGCAC 1 cut(s) 1512
VpaK11BI GGWCC 3 cut(s) 340, 944, 1635
XapI RAATTY 5 cut(s) 307, 783, 958, 1179, 1361
XbaI TCTAGA 1 cut(s) 1235
XceI RCATGY 3 cut(s) 129, 206, 733
XhoI CTCGAG 1 cut(s) 876
XmiI GTMKAC 1 cut(s) 1156
XspI CTAG 3 cut(s) 1146, 1236, 1625
Zsp2I ATGCAT 2 cut(s) 622, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.