Rroxscaffold_4G00282920

aminotransferase ACS12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015320
Physical Location & Seq
Forward (+)
5009243 .. 5011890
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282920.1

Sequence Viewer

Length: 1593 bp
ATGACCCGAACCCGCAAACCCGAAACCTCCTCCTCCGACAGCGACGAAAAGCCCGCCGCATCCACCACCGCCATGAGACTCATCGTCCCTCTCCAAGGCGTCGTCCAAGGCCGCGGAGGCCTCATCCTCGGCTCTCTCATCCCCTGCGCTCTCTTCTACTTCCTCCAGCTCTACCTCAAACGTCACCGTCCCTCCCGACCCGACCCGGACCCGCCTTCTCCCTCCTCCTCCAACCTCCCCGAGCTCCACCGATCCTCCTCGCGCTCCAGCCTGTCCGGCCGCGGATCCATCGGCCGGGTCCGAGTCTCCTCCCGGGCCGACCCGATTGCCAAGCCCAACGAGTCGCCGTATTATATCGGGCTGGATAGGGTTCTGGATGACCCGTATCATAGTGTGGATAATCCGAATGGGGTTATTCAGCTTGGATTGTCTGAAAATAGGCTGTGTTTTGATTTGATTGAGAAATGGGTGTCGGAGAATTGGACGGAATCGATATTGGGAACCAACGGTGGTGATTTGAGCATTGCTGGGATTGCGGCTTACCAGCCGTTTGATGGATTTACTGAGCTGAAAGTGGCCATGGCTAATTTTATGTCCCAGGTGATGCGAAGATCAGTGCCATTTGATCCGTTGCAAGTAGTGTTAACAGCTGGTGCAACCCCGGCAGTTGAGATATTGAGTTTCTGCTTAGCAGACCATGGAAATGCATTTCTTGTTCCTACACCGTATTATCCGGGTTTTGACAGGGATTTGAGATGGCGAACAGGAGTGGAGCTTATTCCTGTTCACTGTCGCAGCACTGACAACTTCACTTTAAATATAACCGTTCTTGAACAAGCATACAGTCAGGCTAGAAAACGAGGGGTGAAAGTACAGGGAATTCTAATTTCTAACCCTTCAAATCCTGTTGGCAATTTAGTTTCAAGGGAAACACTGTGCAGTCTTCTAGACTTTGCCCAAGAGAAGAACATCCATATTATCTCTGATGAGATCTTTGCTGGGTCTATGTATGGAAGCGAGGAGTTTGTGAGCATGGCAGAAATTGTTGAGACAGAAGATTTTGAGAAGAACAGAGTTCACATAATATATGGGTTATCAAAGGACCTATCTATTCCGGGCTTTAGGATTGGGGTTATATATTCATATAATGATAGTGTTCTGGCCGCTGCAAAAAGGTTAACAAGATTTTCTCCCATTTCTGCTCTGACGCAGCGCCTTATTATTTCAATGCTCTTGGATGCTGGATTCATTCAGGAATACCTAGACATCAACAAAAGGAGGATACAACATATGTACGATTTATTTGTGGATGGTTTGCAACAATTAGGAATCAGGTGTGCAAAGAGCAGTGCTGGTTTATACTGTTGGGCCGATATGAGTGGGTTAATTCCCTCCTATGGTGAGAAAGGGGAACTCGAATTATGGGATAACCTGCTGAACATTGCCAAGATCAACGTGACTCCTGGGTCAGCCTGCCACTGCATAGAACCAGGATGGTTCAGGTGTTGTTTTACCAGCTTGATGCCTGAGGATGTTCCTGTAGTTATAGATCGAATCCGAAAAGTTACTGAAACAATTAAATCTTCCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

530

Amino Acids

58.74

Weight (kDa)

6.05

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 139 - 519 9.4e-82 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51690 AT5G51690
fragaria_vesca FvH4_7g28260
malus_domestica MD01G1186400.v1.1
prunus_persica Prupe.2G283100_v2.0.a1
pyrus_communis pycom01g19800
rosa_chinensis RchiOBHm_Chr1g0375231
rosa_laevigata RLG00000026677
rosa_multiflora Rmu_co8467605.1_g000001
rosa_roxburghii Rroxscaffold_4G00282920
rosa_rugosa Rorug01G0389200
rosa_samantha Rh1AG399900 Rh1BG364500 Rh1CG377500 Rh1DG395100
rosa_wichuraiana Rw1G035500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1465
Acc36I ACCTGC 1 cut(s) 1440
AccII CGCG 3 cut(s) 114, 262, 282
AclWI GGATC 4 cut(s) 246, 279, 292, 620
AcoI YGGCCR 4 cut(s) 277, 292, 576, 1161
AcsI RAATTY 1 cut(s) 879
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 2 cut(s) 873, 1295
AfiI CCNNNNNNNGG 4 cut(s) 95, 294, 554, 1397
AgsI TTSAA 4 cut(s) 833, 900, 924, 1227
AhdI GACNNNNNGTC 1 cut(s) 83
AjnI CCWGG 3 cut(s) 597, 1462, 1489
AluBI AGCT 7 cut(s) 169, 244, 421, 568, 650, 775, 1518
AluI AGCT 7 cut(s) 169, 244, 421, 568, 650, 775, 1518
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 3 cut(s) 70, 310, 1043
AlwI GGATC 4 cut(s) 246, 279, 292, 620
Ama87I CYCGRG 2 cut(s) 239, 312
AoxI GGCC 8 cut(s) 109, 118, 277, 292, 315, 576, 1161, 1368
ApeKI GCWGC 3 cut(s) 795, 1166, 1210
ApoI RAATTY 1 cut(s) 879
AspLEI GCGC 3 cut(s) 149, 264, 1215
AspS9I GGNCC 5 cut(s) 208, 298, 315, 1102, 1368
AsuC2I CCSGG 7 cut(s) 206, 296, 313, 314, 662, 735, 1116
AsuHPI GGTGA 5 cut(s) 176, 524, 613, 877, 1412
AvaI CYCGRG 2 cut(s) 239, 312
AvaII GGWCC 3 cut(s) 208, 298, 1102
AxyI CCTNAGG 1 cut(s) 1527
BalI TGGCCA 1 cut(s) 578
BamHI GGATCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 935
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 807, 1153, 1222
BccI CCATC 5 cut(s) 296, 548, 750, 1304, 1488
BceAI ACGGC 2 cut(s) 331, 532
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 3 cut(s) 599, 1464, 1491
BciVI GTATCC 1 cut(s) 1275
BcnI CCSGG 7 cut(s) 206, 296, 313, 314, 662, 735, 1116
BcoDI GTCTC 3 cut(s) 70, 310, 1043
BfaI CTAG 3 cut(s) 852, 947, 1262
BfmI CTRYAG 1 cut(s) 1539
BfoI RGCGCY 1 cut(s) 1216
BfuAI ACCTGC 1 cut(s) 1440
BfuI GTATCC 1 cut(s) 1275
BglI GCCNNNNNGGC 2 cut(s) 117, 276
BglII AGATCT 1 cut(s) 990
BisI GCNGC 8 cut(s) 57, 112, 280, 537, 796, 1164, 1167, 1211
BlpI GCTNAGC 1 cut(s) 688
BlsI GCNGC 8 cut(s) 58, 113, 281, 538, 797, 1165, 1168, 1212
Bme18I GGWCC 3 cut(s) 208, 298, 1102
BmeRI GACNNNNNGTC 1 cut(s) 83
BmeT110I CYCGRG 2 cut(s) 239, 312
BmgT120I GGNCC 5 cut(s) 208, 298, 315, 1102, 1368
BmiI GGNNCC 4 cut(s) 210, 286, 299, 502
BmsI GCATC 4 cut(s) 68, 594, 1228, 1512
BpiI GAAGAC 1 cut(s) 935
BpmI CTGGAG 2 cut(s) 149, 250
Bpu1102I GCTNAGC 1 cut(s) 688
BpuMI CCSGG 7 cut(s) 206, 296, 313, 314, 662, 735, 1116
Bsa29I ATCGAT 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 99
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 239, 269
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 294, 554, 1397
Bse1I ACTGG 1 cut(s) 1587
Bse21I CCTNAGG 1 cut(s) 1527
Bse3DI GCAATG 2 cut(s) 522, 1440
BseBI CCWGG 3 cut(s) 599, 1464, 1491
BseCI ATCGAT 1 cut(s) 491
BseGI GGATG 9 cut(s) 59, 123, 138, 382, 969, 1243, 1315, 1499, 1537
BseLI CCNNNNNNNGG 4 cut(s) 95, 294, 554, 1397
BseMI GCAATG 2 cut(s) 522, 1440
BseMII CTCAG 2 cut(s) 555, 1518
BseNI ACTGG 1 cut(s) 1587
BseRI GAGGAG 7 cut(s) 19, 22, 214, 217, 247, 298, 1034
BseX3I CGGCCG 2 cut(s) 277, 292
BseXI GCAGC 3 cut(s) 807, 1153, 1222
BseYI CCCAGC 2 cut(s) 527, 998
BsgI GTGCAG 1 cut(s) 958
Bsh1236I CGCG 3 cut(s) 114, 262, 282
Bsh1285I CGRYCG 2 cut(s) 280, 295
BshFI GGCC 8 cut(s) 111, 120, 279, 294, 317, 578, 1163, 1370
BshVI ATCGAT 1 cut(s) 491
BsiEI CGRYCG 2 cut(s) 280, 295
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 2 cut(s) 239, 312
BsiSI CCGG 7 cut(s) 206, 276, 295, 313, 662, 734, 1115
BslFI GGGAC 3 cut(s) 71, 174, 580
BslI CCNNNNNNNGG 4 cut(s) 95, 294, 554, 1397
BsmAI GTCTC 3 cut(s) 70, 310, 1043
BsmFI GGGAC 3 cut(s) 71, 174, 580
BsnI GGCC 8 cut(s) 111, 120, 279, 294, 317, 578, 1163, 1370
BsoBI CYCGRG 2 cut(s) 239, 312
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 7 cut(s) 251, 284, 611, 625, 990, 1449, 1549
Bsp1720I GCTNAGC 1 cut(s) 688
Bsp19I CCATGG 2 cut(s) 579, 697
BspANI GGCC 8 cut(s) 111, 120, 279, 294, 317, 578, 1163, 1370
BspCNI CTCAG 2 cut(s) 556, 1519
BspDI ATCGAT 1 cut(s) 491
BspFNI CGCG 3 cut(s) 114, 262, 282
BspLI GGNNCC 4 cut(s) 210, 286, 299, 502
BspMI ACCTGC 1 cut(s) 1440
BspPI GGATC 4 cut(s) 246, 279, 292, 620
BsrDI GCAATG 2 cut(s) 522, 1440
BsrI ACTGG 1 cut(s) 1587
BssMI GATC 7 cut(s) 251, 284, 611, 625, 990, 1449, 1549
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 4 cut(s) 94, 106, 579, 697
Bst2UI CCWGG 3 cut(s) 599, 1464, 1491
Bst4CI ACNGT 8 cut(s) 188, 509, 726, 791, 826, 845, 936, 1364
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 54, 1474
BstDEI CTNAG 3 cut(s) 564, 688, 1527
BstDSI CCRYGG 4 cut(s) 112, 280, 579, 697
BstENI CCTNNNNNAGG 1 cut(s) 93
BstF5I GGATG 9 cut(s) 59, 123, 138, 382, 969, 1243, 1315, 1499, 1537
BstFNI CGCG 3 cut(s) 114, 262, 282
BstH2I RGCGCY 1 cut(s) 1216
BstHHI GCGC 3 cut(s) 149, 264, 1215
BstKTI GATC 7 cut(s) 254, 287, 614, 628, 993, 1452, 1552
BstMAI GTCTC 3 cut(s) 70, 310, 1043
BstMBI GATC 7 cut(s) 251, 284, 611, 625, 990, 1449, 1549
BstMCI CGRYCG 2 cut(s) 280, 295
BstMWI GCNNNNNNNGC 4 cut(s) 117, 276, 533, 662
BstNI CCWGG 3 cut(s) 599, 1464, 1491
BstSFI CTRYAG 1 cut(s) 1539
BstUI CGCG 3 cut(s) 114, 262, 282
BstV1I GCAGC 3 cut(s) 807, 1153, 1222
BstV2I GAAGAC 1 cut(s) 935
BstX2I RGATCY 2 cut(s) 284, 990
BstYI RGATCY 2 cut(s) 284, 990
BstZI CGGCCG 2 cut(s) 277, 292
Bsu15I ATCGAT 1 cut(s) 491
Bsu36I CCTNAGG 1 cut(s) 1527
BsuI GTATCC 1 cut(s) 1275
BsuRI GGCC 8 cut(s) 111, 120, 279, 294, 317, 578, 1163, 1370
BsuTUI ATCGAT 1 cut(s) 491
BtgI CCRYGG 4 cut(s) 112, 280, 579, 697
BtsCI GGATG 9 cut(s) 59, 123, 138, 382, 969, 1243, 1315, 1499, 1537
BtsI GCAGTG 2 cut(s) 1354, 1477
BtsIMutI CAGTG 6 cut(s) 621, 787, 798, 932, 1354, 1477
BveI ACCTGC 1 cut(s) 1440
Cac8I GCNNGC 2 cut(s) 54, 1474
CfoI GCGC 3 cut(s) 149, 264, 1215
Cfr13I GGNCC 5 cut(s) 208, 298, 315, 1102, 1368
Cfr42I CCGCGG 2 cut(s) 115, 283
Cfr9I CCCGGG 1 cut(s) 312
ClaI ATCGAT 1 cut(s) 491
CseI GACGC 2 cut(s) 88, 1216
Csp6I GTAC 2 cut(s) 872, 1294
CviAII CATG 4 cut(s) 73, 580, 698, 1033
CviQI GTAC 2 cut(s) 872, 1294
DdeI CTNAG 3 cut(s) 564, 688, 1527
DpnI GATC 7 cut(s) 253, 286, 613, 627, 992, 1451, 1551
DpnII GATC 7 cut(s) 251, 284, 611, 625, 990, 1449, 1549
DraI TTTAAA 1 cut(s) 816
DrdI GACNNNNNNGTC 1 cut(s) 1465
DriI GACNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 1465
EaeI YGGCCR 4 cut(s) 277, 292, 576, 1161
EagI CGGCCG 2 cut(s) 277, 292
Eam1104I CTCTTC 1 cut(s) 158
Eam1105I GACNNNNNGTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 158
Ecl136II GAGCTC 1 cut(s) 244
EclXI CGGCCG 2 cut(s) 277, 292
Eco130I CCWWGG 4 cut(s) 94, 106, 579, 697
Eco147I AGGCCT 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 3 cut(s) 208, 298, 1102
Eco52I CGGCCG 2 cut(s) 277, 292
Eco53kI GAGCTC 1 cut(s) 244
Eco81I CCTNAGG 1 cut(s) 1527
Eco88I CYCGRG 2 cut(s) 239, 312
EcoICRI GAGCTC 1 cut(s) 244
EcoNI CCTNNNNNAGG 1 cut(s) 93
EcoO109I RGGNCCY 1 cut(s) 1102
EcoRI GAATTC 1 cut(s) 879
EcoRII CCWGG 3 cut(s) 597, 1462, 1489
EcoT14I CCWWGG 4 cut(s) 94, 106, 579, 697
EcoT22I ATGCAT 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 4 cut(s) 94, 106, 579, 697
FaeI CATG 4 cut(s) 76, 583, 701, 1036
FaqI GGGAC 3 cut(s) 71, 174, 580
FatI CATG 4 cut(s) 72, 579, 697, 1032
FauI CCCGC 3 cut(s) 20, 61, 219
FauNDI CATATG 1 cut(s) 1290
Fnu4HI GCNGC 8 cut(s) 57, 112, 280, 537, 796, 1164, 1167, 1211
FokI GGATG 9 cut(s) 46, 110, 125, 389, 956, 1250, 1322, 1506, 1544
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 8 cut(s) 57, 112, 280, 537, 796, 1164, 1167, 1211
FspBI CTAG 3 cut(s) 852, 947, 1262
GlaI GCGC 3 cut(s) 148, 263, 1214
GluI GCNGC 8 cut(s) 57, 112, 280, 537, 796, 1164, 1167, 1211
GsaI CCCAGC 2 cut(s) 531, 1002
GsuI CTGGAG 2 cut(s) 149, 250
HaeII RGCGCY 1 cut(s) 1216
HaeIII GGCC 8 cut(s) 111, 120, 279, 294, 317, 578, 1163, 1370
HapII CCGG 7 cut(s) 206, 276, 295, 313, 662, 734, 1115
HgaI GACGC 2 cut(s) 88, 1216
HhaI GCGC 3 cut(s) 149, 264, 1215
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 4 cut(s) 76, 583, 701, 1036
Hin6I GCGC 3 cut(s) 147, 262, 1213
HinP1I GCGC 3 cut(s) 147, 262, 1213
HincII GTYRAC 2 cut(s) 645, 1179
HindII GTYRAC 2 cut(s) 645, 1179
HinfI GANTC 8 cut(s) 78, 303, 341, 488, 1245, 1329, 1459, 1554
HpaI GTTAAC 2 cut(s) 645, 1179
HpaII CCGG 7 cut(s) 206, 276, 295, 313, 662, 734, 1115
HphI GGTGA 5 cut(s) 176, 524, 613, 877, 1412
Hpy166II GTNNAC 4 cut(s) 645, 787, 1078, 1179
Hpy188I TCNGA 8 cut(s) 37, 302, 405, 433, 475, 985, 1206, 1559
Hpy188III TCNNGA 5 cut(s) 195, 374, 830, 947, 1253
Hpy8I GTNNAC 4 cut(s) 645, 787, 1078, 1179
Hpy99I CGWCG 2 cut(s) 47, 104
HpyAV CCTTC 2 cut(s) 225, 906
HpyCH4III ACNGT 8 cut(s) 188, 509, 726, 791, 826, 845, 936, 1364
HpyCH4IV ACGT 2 cut(s) 181, 1455
HpyCH4V TGCA 8 cut(s) 634, 656, 707, 939, 1169, 1318, 1340, 1482
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 276, 533, 662
HpyF3I CTNAG 3 cut(s) 564, 688, 1527
HpySE526I ACGT 2 cut(s) 181, 1455
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 4 cut(s) 76, 583, 701, 1036
HspAI GCGC 3 cut(s) 147, 262, 1213
KspAI GTTAAC 2 cut(s) 645, 1179
KspI CCGCGG 2 cut(s) 115, 283
Kzo9I GATC 7 cut(s) 251, 284, 611, 625, 990, 1449, 1549
LmnI GCTCC 3 cut(s) 249, 269, 772
Lsp1109I GCAGC 3 cut(s) 807, 1153, 1222
LweI GCATC 4 cut(s) 68, 594, 1228, 1512
MaeI CTAG 3 cut(s) 852, 947, 1262
MaeII ACGT 2 cut(s) 181, 1455
MaeIII GTNAC 3 cut(s) 182, 1456, 1564
MalI GATC 7 cut(s) 253, 286, 613, 627, 992, 1451, 1551
MboI GATC 7 cut(s) 251, 284, 611, 625, 990, 1449, 1549
MboII GAAGA 7 cut(s) 145, 621, 935, 976, 1067, 1078, 1575
MflI RGATCY 2 cut(s) 284, 990
MhlI GDGCHC 1 cut(s) 246
MlsI TGGCCA 1 cut(s) 578
MluNI TGGCCA 1 cut(s) 578
MlyI GAGTC 4 cut(s) 72, 312, 350, 1453
MmeI TCCRAC 3 cut(s) 60, 255, 453
Mox20I TGGCCA 1 cut(s) 578
Mph1103I ATGCAT 1 cut(s) 709
MscI TGGCCA 1 cut(s) 578
MseI TTAA 5 cut(s) 644, 815, 1178, 1385, 1578
MslI CAYNNNNRTG 2 cut(s) 71, 702
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 4 cut(s) 114, 282, 650, 1166
MspI CCGG 7 cut(s) 206, 276, 295, 313, 662, 734, 1115
MvaI CCWGG 3 cut(s) 599, 1464, 1491
MvnI CGCG 3 cut(s) 114, 262, 282
MwoI GCNNNNNNNGC 4 cut(s) 117, 276, 533, 662
NciI CCSGG 7 cut(s) 206, 296, 313, 314, 662, 735, 1116
NcoI CCATGG 2 cut(s) 579, 697
NdeI CATATG 1 cut(s) 1290
NdeII GATC 7 cut(s) 251, 284, 611, 625, 990, 1449, 1549
NlaIII CATG 4 cut(s) 76, 583, 701, 1036
NlaIV GGNNCC 4 cut(s) 210, 286, 299, 502
NmeAIII GCCGAG 1 cut(s) 108
NmuCI GTSAC 2 cut(s) 182, 1456
NsiI ATGCAT 1 cut(s) 709
PceI AGGCCT 1 cut(s) 120
PfeI GAWTC 4 cut(s) 488, 1245, 1329, 1554
PkrI GCNGC 8 cut(s) 58, 113, 281, 538, 797, 1165, 1168, 1212
PleI GAGTC 4 cut(s) 72, 311, 349, 1453
PpsI GAGTC 4 cut(s) 72, 311, 349, 1453
PpuMI RGGWCCY 1 cut(s) 1102
Psp124BI GAGCTC 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 1102
Psp6I CCWGG 3 cut(s) 597, 1462, 1489
PspFI CCCAGC 2 cut(s) 527, 998
PspGI CCWGG 3 cut(s) 597, 1462, 1489
PspN4I GGNNCC 4 cut(s) 210, 286, 299, 502
PspPI GGNCC 5 cut(s) 208, 298, 315, 1102, 1368
PspPPI RGGWCCY 1 cut(s) 1102
PsuI RGATCY 2 cut(s) 284, 990
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 2 cut(s) 873, 1295
RsaNI GTAC 2 cut(s) 872, 1294
RseI CAYNNNNRTG 2 cut(s) 71, 702
SacI GAGCTC 1 cut(s) 246
SacII CCGCGG 2 cut(s) 115, 283
SaqAI TTAA 5 cut(s) 644, 815, 1178, 1385, 1578
SatI GCNGC 8 cut(s) 57, 112, 280, 537, 796, 1164, 1167, 1211
Sau3AI GATC 7 cut(s) 251, 284, 611, 625, 990, 1449, 1549
Sau96I GGNCC 5 cut(s) 208, 298, 315, 1102, 1368
SchI GAGTC 4 cut(s) 72, 312, 350, 1453
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 4 cut(s) 68, 594, 1228, 1512
SfcI CTRYAG 1 cut(s) 1539
SfiI GGCCNNNNNGGCC 1 cut(s) 117
Sfr303I CCGCGG 2 cut(s) 115, 283
SgrBI CCGCGG 2 cut(s) 115, 283
SinI GGWCC 3 cut(s) 208, 298, 1102
SmaI CCCGGG 1 cut(s) 314
SmiMI CAYNNNNRTG 2 cut(s) 71, 702
SseBI AGGCCT 1 cut(s) 120
SspMI CTAG 3 cut(s) 852, 947, 1262
SstI GAGCTC 1 cut(s) 246
StuI AGGCCT 1 cut(s) 120
StyI CCWWGG 4 cut(s) 94, 106, 579, 697
TaaI ACNGT 8 cut(s) 188, 509, 726, 791, 826, 845, 936, 1364
TaiI ACGT 2 cut(s) 184, 1458
TaqI TCGA 3 cut(s) 491, 1416, 1552
TatI WGTACW 1 cut(s) 871
TauI GCSGC 5 cut(s) 59, 114, 282, 539, 1166
TfiI GAWTC 4 cut(s) 488, 1245, 1329, 1554
Tru1I TTAA 5 cut(s) 644, 815, 1178, 1385, 1578
Tru9I TTAA 5 cut(s) 644, 815, 1178, 1385, 1578
TscAI CASTG 6 cut(s) 621, 794, 805, 939, 1354, 1484
TseFI GTSAC 2 cut(s) 182, 1456
TseI GCWGC 3 cut(s) 795, 1166, 1210
Tsp45I GTSAC 2 cut(s) 182, 1456
TspDTI ATGAA 2 cut(s) 1131, 1237
TspGWI ACGGA 2 cut(s) 500, 618
TspMI CCCGGG 1 cut(s) 312
TspRI CASTG 6 cut(s) 621, 794, 805, 939, 1354, 1484
VpaK11BI GGWCC 3 cut(s) 208, 298, 1102
XagI CCTNNNNNAGG 1 cut(s) 93
XapI RAATTY 1 cut(s) 879
XbaI TCTAGA 1 cut(s) 946
XcmI CCANNNNNNNNNTGG 1 cut(s) 551
XmaI CCCGGG 1 cut(s) 312
XspI CTAG 3 cut(s) 852, 947, 1262
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.