Rw1G035500

aminotransferase ACS12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015320
Physical Location & Seq
Reverse (-)
63264899 .. 63268871
3973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035500.1

Sequence Viewer

Length: 1602 bp
ATGACCCGAACCCGCAAACCCGAAACCTCCTCCTCCGACGAAGACAACAACGACGAAAAGCCCGCCGCATCCACCACCGCCATGAGACTCATCGTCCCTCTCCAAGGCGTCGTCCAAGGCCGCGGAGGCCTCATCCTCGGCTCTCTCATCCCCTGCGCTCTCTTCTACTTCCTCCAGCTCTACCTCAAACGTCACCGTCCCTCCCAACCCGACCCGGACCCGCCTTCTCCCTCCTCCTCCAACCTCCCCGAGCTCCATCGATCCTCCTCGCGCTCCAGCTTGTCCGGCCGCGGATCCATCGGCCGGGTCCGGGTCTCCTCCCGGGCCGACCCGATTGCCAAGCCCAACGAGTCGCCGTATTATATCGGGCTGGATAGGGTTCTGGATGACCCGTATCATAGTGTGGATAATCCGAATGGGGTTATTCAGCTCGGATTGTCTGAAAATAGGCTGTGTTTTGATTTGATTGAGAAATGGGTGTCGGAGAATTGGACGGAGTCGATATTGGGAACCAACGGTGGTGATTTGAGCATTGCCGGGATTGCGGCGTACCAGCCGTTTGATGGATTTGCTGAGCTGAAAGTGGCTATGGCTAATTTCATGTCCCAGGTGATGCGAAGATCAGTGCCATTTGATCCGTCGCAACTAGTGTTAACAGCTGGTGCAACCCCGGCAGTTGAGATATTGAGTTTCTGCTTAGCAGACCATGGAAATGCATTTCTTGTTCCTACGCCGTATTATCCGGGTTTTGACCGGGATTTGAGATGGCGAACAGGAGTGGAGCTTATTCCCGTTCACTGTCGCAGCACTGACAACTTCACTTTAAATATAACCGTTCTTGAACAAGCATACAGTCAGGCTAGAAAACGAGGGGTGAAAGTACAGGGAATTCTAATTTCTAACCCTTCGAATCCTGTTGGCAATTTAGTTTCAAGGGAAACACTGTGCAGTCTTCTAGACTTTGCCCAAGAGAAGAACATTCATATTATCTCTGATGAGATCTTTGCTGGGTCTATGTATGGAAGCGAGGAGTTTGTGAGCATGGCAGAAATTGTTGAGACAGAAGATTTTGAGAAGAACAGAGTTCACATAATATATGGGTTATCAAAGGACCTATCTATTCCGGGCTTTAGGATTGGGGTTATATATTCATATAATGATAGTGTTCTGGCCGCTGCAAAAAGGTTAACAAGATTTTCTCCCATTTCTGCTCCGACGCAGCGCCTTATTATTTCAATGCTCTTGGATACTGGATTCATTCAGGAATACCTAGACATCAACAAAAGGAGGATACAACATATGTACGATTTATTTGTGGATGGTTTGCAACAATTAGGAATCAAGTGTGCAAAGAGCAGTGCTGGTTTATACTGTTGGGCCGATATGAGTGGGTTAATTCCCTCCTATGGTGAGAAAGGGGAACTCGAATTATGGGATAACCTGCTGAACATTGCCAAGATCAACGTGACTCCTGGGTCAGCCTGCCACTGCATAGAACCAGGATGGTTCAGGTGTTGTTTTACCAGCTTGATGCCTGAGGATGTTCCTGTAGTTATAGATCGAATCCGAAAAGTTACTGAAACAATTAAATCTTCCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

533

Amino Acids

59.01

Weight (kDa)

5.71

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 142 - 522 9.2e-83 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51690 AT5G51690
fragaria_vesca FvH4_7g28260
malus_domestica MD01G1186400.v1.1
prunus_persica Prupe.2G283100_v2.0.a1
pyrus_communis pycom01g19800
rosa_chinensis RchiOBHm_Chr1g0375231
rosa_laevigata RLG00000026677
rosa_multiflora Rmu_co8467605.1_g000001
rosa_roxburghii Rroxscaffold_4G00282920
rosa_rugosa Rorug01G0389200
rosa_samantha Rh1AG399900 Rh1BG364500 Rh1CG377500 Rh1DG395100
rosa_wichuraiana Rw1G035500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1474
Acc36I ACCTGC 1 cut(s) 1449
AccII CGCG 3 cut(s) 123, 271, 291
AclWI GGATC 4 cut(s) 255, 288, 301, 629
AcoI YGGCCR 3 cut(s) 286, 301, 1170
AcsI RAATTY 1 cut(s) 888
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 3 cut(s) 551, 882, 1304
AfiI CCNNNNNNNGG 5 cut(s) 104, 303, 310, 563, 1406
AgsI TTSAA 3 cut(s) 842, 933, 1236
AhdI GACNNNNNGTC 1 cut(s) 92
AhlI ACTAGT 1 cut(s) 646
AjnI CCWGG 3 cut(s) 606, 1471, 1498
AluBI AGCT 8 cut(s) 178, 253, 279, 430, 577, 659, 784, 1527
AluI AGCT 8 cut(s) 178, 253, 279, 430, 577, 659, 784, 1527
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 3 cut(s) 79, 319, 1052
AlwI GGATC 4 cut(s) 255, 288, 301, 629
Ama87I CYCGRG 2 cut(s) 248, 321
AoxI GGCC 7 cut(s) 118, 127, 286, 301, 324, 1170, 1377
ApeKI GCWGC 3 cut(s) 804, 1175, 1219
ApoI RAATTY 1 cut(s) 888
AspLEI GCGC 3 cut(s) 158, 273, 1224
AspS9I GGNCC 5 cut(s) 217, 307, 324, 1111, 1377
AsuHPI GGTGA 5 cut(s) 185, 533, 622, 886, 1421
AsuII TTCGAA 1 cut(s) 908
AvaI CYCGRG 2 cut(s) 248, 321
AvaII GGWCC 3 cut(s) 217, 307, 1111
AxyI CCTNAGG 1 cut(s) 1536
BamHI GGATCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 255
BbsI GAAGAC 2 cut(s) 48, 944
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 3 cut(s) 816, 1162, 1231
BccI CCATC 6 cut(s) 264, 305, 557, 759, 1313, 1497
BceAI ACGGC 3 cut(s) 340, 541, 718
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 3 cut(s) 608, 1473, 1500
BciVI GTATCC 2 cut(s) 1240, 1284
BcoDI GTCTC 3 cut(s) 79, 319, 1052
BcuI ACTAGT 1 cut(s) 646
BfaI CTAG 4 cut(s) 647, 861, 956, 1271
BfmI CTRYAG 1 cut(s) 1548
BfoI RGCGCY 1 cut(s) 1225
BfuAI ACCTGC 1 cut(s) 1449
BfuI GTATCC 2 cut(s) 1240, 1284
BglI GCCNNNNNGGC 1 cut(s) 126
BglII AGATCT 1 cut(s) 999
BisI GCNGC 8 cut(s) 66, 121, 289, 546, 805, 1173, 1176, 1220
BlpI GCTNAGC 2 cut(s) 573, 697
BlsI GCNGC 8 cut(s) 67, 122, 290, 547, 806, 1174, 1177, 1221
Bme18I GGWCC 3 cut(s) 217, 307, 1111
BmeRI GACNNNNNGTC 1 cut(s) 92
BmeT110I CYCGRG 2 cut(s) 248, 321
BmgT120I GGNCC 5 cut(s) 217, 307, 324, 1111, 1377
BmiI GGNNCC 4 cut(s) 219, 295, 308, 511
BmsI GCATC 3 cut(s) 77, 603, 1521
BpiI GAAGAC 2 cut(s) 48, 944
BpmI CTGGAG 2 cut(s) 158, 259
Bpu1102I GCTNAGC 2 cut(s) 573, 697
Bpu14I TTCGAA 1 cut(s) 908
Bsa29I ATCGAT 1 cut(s) 259
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 319
BsaWI WCCGGW 1 cut(s) 1595
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 303, 310, 563, 1406
Bse1I ACTGG 1 cut(s) 1255
Bse21I CCTNAGG 1 cut(s) 1536
Bse3DI GCAATG 2 cut(s) 531, 1449
BseBI CCWGG 3 cut(s) 608, 1473, 1500
BseCI ATCGAT 1 cut(s) 259
BseGI GGATG 7 cut(s) 68, 132, 147, 391, 1324, 1508, 1546
BseLI CCNNNNNNNGG 5 cut(s) 104, 303, 310, 563, 1406
BseMI GCAATG 2 cut(s) 531, 1449
BseMII CTCAG 2 cut(s) 564, 1527
BseNI ACTGG 1 cut(s) 1255
BseRI GAGGAG 7 cut(s) 19, 22, 223, 226, 256, 307, 1043
BseX3I CGGCCG 2 cut(s) 286, 301
BseXI GCAGC 3 cut(s) 816, 1162, 1231
BseYI CCCAGC 1 cut(s) 1007
BsgI GTGCAG 1 cut(s) 967
Bsh1236I CGCG 3 cut(s) 123, 271, 291
Bsh1285I CGRYCG 2 cut(s) 289, 304
BshFI GGCC 7 cut(s) 120, 129, 288, 303, 326, 1172, 1379
BshVI ATCGAT 1 cut(s) 259
BsiEI CGRYCG 2 cut(s) 289, 304
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 2 cut(s) 248, 321
BslFI GGGAC 3 cut(s) 80, 183, 589
BslI CCNNNNNNNGG 5 cut(s) 104, 303, 310, 563, 1406
BsmAI GTCTC 3 cut(s) 79, 319, 1052
BsmFI GGGAC 3 cut(s) 80, 183, 589
BsnI GGCC 7 cut(s) 120, 129, 288, 303, 326, 1172, 1379
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 2 cut(s) 248, 321
Bsp119I TTCGAA 1 cut(s) 908
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 7 cut(s) 260, 293, 620, 634, 999, 1458, 1558
Bsp1720I GCTNAGC 2 cut(s) 573, 697
Bsp19I CCATGG 1 cut(s) 706
BspANI GGCC 7 cut(s) 120, 129, 288, 303, 326, 1172, 1379
BspCNI CTCAG 2 cut(s) 565, 1528
BspDI ATCGAT 1 cut(s) 259
BspFNI CGCG 3 cut(s) 123, 271, 291
BspLI GGNNCC 4 cut(s) 219, 295, 308, 511
BspMI ACCTGC 1 cut(s) 1449
BspPI GGATC 4 cut(s) 255, 288, 301, 629
BspT104I TTCGAA 1 cut(s) 908
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 2 cut(s) 531, 1449
BsrI ACTGG 1 cut(s) 1255
BssMI GATC 7 cut(s) 260, 293, 620, 634, 999, 1458, 1558
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 3 cut(s) 103, 115, 706
Bst2UI CCWGG 3 cut(s) 608, 1473, 1500
Bst4CI ACNGT 7 cut(s) 197, 518, 800, 835, 854, 945, 1373
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 108
BstBI TTCGAA 1 cut(s) 908
BstC8I GCNNGC 2 cut(s) 63, 1483
BstDEI CTNAG 3 cut(s) 573, 697, 1536
BstDSI CCRYGG 3 cut(s) 121, 289, 706
BstENI CCTNNNNNAGG 1 cut(s) 102
BstF5I GGATG 7 cut(s) 68, 132, 147, 391, 1324, 1508, 1546
BstFNI CGCG 3 cut(s) 123, 271, 291
BstH2I RGCGCY 1 cut(s) 1225
BstHHI GCGC 3 cut(s) 158, 273, 1224
BstKTI GATC 7 cut(s) 263, 296, 623, 637, 1002, 1461, 1561
BstMAI GTCTC 3 cut(s) 79, 319, 1052
BstMBI GATC 7 cut(s) 260, 293, 620, 634, 999, 1458, 1558
BstMCI CGRYCG 2 cut(s) 289, 304
BstMWI GCNNNNNNNGC 4 cut(s) 126, 285, 542, 671
BstNI CCWGG 3 cut(s) 608, 1473, 1500
BstSFI CTRYAG 1 cut(s) 1548
BstUI CGCG 3 cut(s) 123, 271, 291
BstV1I GCAGC 3 cut(s) 816, 1162, 1231
BstV2I GAAGAC 2 cut(s) 48, 944
BstX2I RGATCY 2 cut(s) 293, 999
BstYI RGATCY 2 cut(s) 293, 999
BstZI CGGCCG 2 cut(s) 286, 301
Bsu15I ATCGAT 1 cut(s) 259
Bsu36I CCTNAGG 1 cut(s) 1536
BsuI GTATCC 2 cut(s) 1240, 1284
BsuRI GGCC 7 cut(s) 120, 129, 288, 303, 326, 1172, 1379
BsuTUI ATCGAT 1 cut(s) 259
BtgI CCRYGG 3 cut(s) 121, 289, 706
BtsCI GGATG 7 cut(s) 68, 132, 147, 391, 1324, 1508, 1546
BtsI GCAGTG 2 cut(s) 1363, 1486
BtsIMutI CAGTG 6 cut(s) 630, 796, 807, 941, 1363, 1486
BveI ACCTGC 1 cut(s) 1449
Cac8I GCNNGC 2 cut(s) 63, 1483
CfoI GCGC 3 cut(s) 158, 273, 1224
Cfr13I GGNCC 5 cut(s) 217, 307, 324, 1111, 1377
Cfr42I CCGCGG 2 cut(s) 124, 292
Cfr9I CCCGGG 1 cut(s) 321
ClaI ATCGAT 1 cut(s) 259
CseI GACGC 2 cut(s) 97, 1225
Csp6I GTAC 3 cut(s) 550, 881, 1303
CviAII CATG 4 cut(s) 82, 601, 707, 1042
CviQI GTAC 3 cut(s) 550, 881, 1303
DdeI CTNAG 3 cut(s) 573, 697, 1536
DpnI GATC 7 cut(s) 262, 295, 622, 636, 1001, 1460, 1560
DpnII GATC 7 cut(s) 260, 293, 620, 634, 999, 1458, 1558
DraI TTTAAA 1 cut(s) 825
DrdI GACNNNNNNGTC 1 cut(s) 1474
DriI GACNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 1474
EaeI YGGCCR 3 cut(s) 286, 301, 1170
EagI CGGCCG 2 cut(s) 286, 301
Eam1104I CTCTTC 1 cut(s) 167
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 253
EclXI CGGCCG 2 cut(s) 286, 301
Eco130I CCWWGG 3 cut(s) 103, 115, 706
Eco147I AGGCCT 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 255
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 3 cut(s) 217, 307, 1111
Eco52I CGGCCG 2 cut(s) 286, 301
Eco53kI GAGCTC 1 cut(s) 253
Eco81I CCTNAGG 1 cut(s) 1536
Eco88I CYCGRG 2 cut(s) 248, 321
EcoICRI GAGCTC 1 cut(s) 253
EcoNI CCTNNNNNAGG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 1111
EcoRI GAATTC 1 cut(s) 888
EcoRII CCWGG 3 cut(s) 606, 1471, 1498
EcoT14I CCWWGG 3 cut(s) 103, 115, 706
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 3 cut(s) 103, 115, 706
FaeI CATG 4 cut(s) 85, 604, 710, 1045
FaqI GGGAC 3 cut(s) 80, 183, 589
FatI CATG 4 cut(s) 81, 600, 706, 1041
FauI CCCGC 3 cut(s) 20, 70, 228
FauNDI CATATG 1 cut(s) 1299
Fnu4HI GCNGC 8 cut(s) 66, 121, 289, 546, 805, 1173, 1176, 1220
FokI GGATG 7 cut(s) 55, 119, 134, 398, 1331, 1515, 1553
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 8 cut(s) 66, 121, 289, 546, 805, 1173, 1176, 1220
FspBI CTAG 4 cut(s) 647, 861, 956, 1271
GlaI GCGC 3 cut(s) 157, 272, 1223
GluI GCNGC 8 cut(s) 66, 121, 289, 546, 805, 1173, 1176, 1220
GsaI CCCAGC 1 cut(s) 1011
GsuI CTGGAG 2 cut(s) 158, 259
HaeII RGCGCY 1 cut(s) 1225
HaeIII GGCC 7 cut(s) 120, 129, 288, 303, 326, 1172, 1379
HgaI GACGC 2 cut(s) 97, 1225
HhaI GCGC 3 cut(s) 158, 273, 1224
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 4 cut(s) 85, 604, 710, 1045
Hin6I GCGC 3 cut(s) 156, 271, 1222
HinP1I GCGC 3 cut(s) 156, 271, 1222
HincII GTYRAC 2 cut(s) 654, 1188
HindII GTYRAC 2 cut(s) 654, 1188
HinfI GANTC 8 cut(s) 87, 350, 497, 910, 1254, 1338, 1468, 1563
HpaI GTTAAC 2 cut(s) 654, 1188
HphI GGTGA 5 cut(s) 185, 533, 622, 886, 1421
Hpy166II GTNNAC 4 cut(s) 654, 796, 1087, 1188
Hpy188I TCNGA 8 cut(s) 37, 414, 434, 442, 484, 994, 1215, 1568
Hpy188III TCNNGA 4 cut(s) 383, 839, 956, 1262
Hpy8I GTNNAC 4 cut(s) 654, 796, 1087, 1188
Hpy99I CGWCG 5 cut(s) 41, 56, 113, 643, 1219
HpyAV CCTTC 2 cut(s) 234, 915
HpyCH4III ACNGT 7 cut(s) 197, 518, 800, 835, 854, 945, 1373
HpyCH4IV ACGT 2 cut(s) 190, 1464
HpyCH4V TGCA 7 cut(s) 665, 716, 948, 1178, 1327, 1349, 1491
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 285, 542, 671
HpyF3I CTNAG 3 cut(s) 573, 697, 1536
HpySE526I ACGT 2 cut(s) 190, 1464
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 4 cut(s) 85, 604, 710, 1045
HspAI GCGC 3 cut(s) 156, 271, 1222
KspAI GTTAAC 2 cut(s) 654, 1188
KspI CCGCGG 2 cut(s) 124, 292
Kzo9I GATC 7 cut(s) 260, 293, 620, 634, 999, 1458, 1558
LmnI GCTCC 4 cut(s) 258, 278, 781, 1216
Lsp1109I GCAGC 3 cut(s) 816, 1162, 1231
LweI GCATC 3 cut(s) 77, 603, 1521
MaeI CTAG 4 cut(s) 647, 861, 956, 1271
MaeII ACGT 2 cut(s) 190, 1464
MaeIII GTNAC 3 cut(s) 191, 1465, 1573
MalI GATC 7 cut(s) 262, 295, 622, 636, 1001, 1460, 1560
MboI GATC 7 cut(s) 260, 293, 620, 634, 999, 1458, 1558
MboII GAAGA 8 cut(s) 53, 154, 630, 944, 985, 1076, 1087, 1584
MflI RGATCY 2 cut(s) 293, 999
MhlI GDGCHC 1 cut(s) 255
MlyI GAGTC 4 cut(s) 81, 359, 506, 1462
MmeI TCCRAC 4 cut(s) 60, 264, 462, 1238
Mph1103I ATGCAT 1 cut(s) 718
MseI TTAA 5 cut(s) 653, 824, 1187, 1394, 1587
MslI CAYNNNNRTG 2 cut(s) 80, 711
MspA1I CMGCKG 4 cut(s) 123, 291, 659, 1175
MvaI CCWGG 3 cut(s) 608, 1473, 1500
MvnI CGCG 3 cut(s) 123, 271, 291
MwoI GCNNNNNNNGC 4 cut(s) 126, 285, 542, 671
NcoI CCATGG 1 cut(s) 706
NdeI CATATG 1 cut(s) 1299
NdeII GATC 7 cut(s) 260, 293, 620, 634, 999, 1458, 1558
NlaIII CATG 4 cut(s) 85, 604, 710, 1045
NlaIV GGNNCC 4 cut(s) 219, 295, 308, 511
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 2 cut(s) 191, 1465
NsiI ATGCAT 1 cut(s) 718
NspV TTCGAA 1 cut(s) 908
PceI AGGCCT 1 cut(s) 129
PfeI GAWTC 4 cut(s) 910, 1254, 1338, 1563
PflFI GACNNNGTC 1 cut(s) 496
PkrI GCNGC 8 cut(s) 67, 122, 290, 547, 806, 1174, 1177, 1221
PleI GAGTC 4 cut(s) 81, 358, 505, 1462
PpsI GAGTC 4 cut(s) 81, 358, 505, 1462
PpuMI RGGWCCY 1 cut(s) 1111
Psp124BI GAGCTC 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 1111
Psp6I CCWGG 3 cut(s) 606, 1471, 1498
PspFI CCCAGC 1 cut(s) 1007
PspGI CCWGG 3 cut(s) 606, 1471, 1498
PspN4I GGNNCC 4 cut(s) 219, 295, 308, 511
PspPI GGNCC 5 cut(s) 217, 307, 324, 1111, 1377
PspPPI RGGWCCY 1 cut(s) 1111
PsuI RGATCY 2 cut(s) 293, 999
PsyI GACNNNGTC 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 3 cut(s) 551, 882, 1304
RsaNI GTAC 3 cut(s) 550, 881, 1303
RseI CAYNNNNRTG 2 cut(s) 80, 711
SacI GAGCTC 1 cut(s) 255
SacII CCGCGG 2 cut(s) 124, 292
SaqAI TTAA 5 cut(s) 653, 824, 1187, 1394, 1587
SatI GCNGC 8 cut(s) 66, 121, 289, 546, 805, 1173, 1176, 1220
Sau3AI GATC 7 cut(s) 260, 293, 620, 634, 999, 1458, 1558
Sau96I GGNCC 5 cut(s) 217, 307, 324, 1111, 1377
SchI GAGTC 4 cut(s) 81, 359, 506, 1462
SduI GDGCHC 1 cut(s) 255
SfaNI GCATC 3 cut(s) 77, 603, 1521
SfcI CTRYAG 1 cut(s) 1548
SfiI GGCCNNNNNGGCC 1 cut(s) 126
Sfr303I CCGCGG 2 cut(s) 124, 292
SfuI TTCGAA 1 cut(s) 908
SgrBI CCGCGG 2 cut(s) 124, 292
SinI GGWCC 3 cut(s) 217, 307, 1111
SmaI CCCGGG 1 cut(s) 323
SmiMI CAYNNNNRTG 2 cut(s) 80, 711
SpeI ACTAGT 1 cut(s) 646
SseBI AGGCCT 1 cut(s) 129
SspMI CTAG 4 cut(s) 647, 861, 956, 1271
SstI GAGCTC 1 cut(s) 255
StuI AGGCCT 1 cut(s) 129
StyI CCWWGG 3 cut(s) 103, 115, 706
TaaI ACNGT 7 cut(s) 197, 518, 800, 835, 854, 945, 1373
TaiI ACGT 2 cut(s) 193, 1467
TaqI TCGA 5 cut(s) 259, 500, 908, 1425, 1561
TatI WGTACW 1 cut(s) 880
TauI GCSGC 5 cut(s) 68, 123, 291, 548, 1175
TfiI GAWTC 4 cut(s) 910, 1254, 1338, 1563
Tru1I TTAA 5 cut(s) 653, 824, 1187, 1394, 1587
Tru9I TTAA 5 cut(s) 653, 824, 1187, 1394, 1587
TscAI CASTG 6 cut(s) 630, 803, 814, 948, 1363, 1493
TseFI GTSAC 2 cut(s) 191, 1465
TseI GCWGC 3 cut(s) 804, 1175, 1219
Tsp45I GTSAC 2 cut(s) 191, 1465
TspDTI ATGAA 4 cut(s) 589, 971, 1140, 1246
TspGWI ACGGA 2 cut(s) 509, 627
TspMI CCCGGG 1 cut(s) 321
TspRI CASTG 6 cut(s) 630, 803, 814, 948, 1363, 1493
Tth111I GACNNNGTC 1 cut(s) 496
VpaK11BI GGWCC 3 cut(s) 217, 307, 1111
XagI CCTNNNNNAGG 1 cut(s) 102
XapI RAATTY 1 cut(s) 888
XbaI TCTAGA 1 cut(s) 955
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XmaI CCCGGG 1 cut(s) 321
XspI CTAG 4 cut(s) 647, 861, 956, 1271
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.