Prupe.2G283100_v2.0.a1

aminotransferase ACS12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0015320
Physical Location & Seq
Reverse (-)
28105099 .. 28107897
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G283100.1

Sequence Viewer

Length: 1527 bp
ATGAGACTGATAGTGCCACTCCAAGGCGTCGTCCAAGGCAGAGGTGGCCTTATCCTCGGCTCTCTTATCCCCTGTGCTCTCTTCTACTTCCTCCAGCTCTACCTAAAACGACACCGTCCCTCCAAACCCCCATCCAGCCCTCCTTCCCCATCCCACTCCTCCTCGAACATCGCCGAGCTCCAGAGAACCTCGTCGCGGTCCAACTTGTCCACCCGTGGATCCATGGGGCGGGTACGGGTATCCTCCCGGGCGAGTTGGATTGCGAAGCCAAACGACTCCCCGTATTATATCGGGTTGGATAGGGTTTTGGAGGACCCGTACGATAGGGTGGACAATCCGAATGGGGTTATTCAGCTCGGGTTGTCCGAGAACAGGCTGTGTTTGGATTTGATTGAGAAATGGATTACGGAGAATTTGACGCAATCAATTTTCGGAGAAGACGGTGGTGATTTTAGCATAAGTGGGATTGCGGCGTACCAGCCGTTCGATGGAATGACTGAGCTCAAAGTGGCTATGGCAAGTTTCATGTCTCAGGTTATGCGAAAATCAGTGTCATTTGACCCATCACAAATAGTGTTAACATCTGGTGCAACCCCTGCAGTTGAGATATTAAGCTTCTGCTTAGCAGACCATGGAAATGCATTTCTCGTTCCTACACCTTATTATCCTGGTTTTCACAGGGATATAAGATGGCGAGCAGGAGTGGAGCTGATTCCCGTTCACTGTCGCAGCACTGACAACTTCGCTTTAAATATAACTGTTCTTGAACAAGCATACAATCAGGCTAGAAAACGAGGAGTAAAAGTGCAGGGAGTTCTCATTTCTAACCCTTCGAATCCCGTTGGTAATTTACTTTCACGGGAAACACTGTGCAGTCTTTTAGACTTTGCCCAAGAGAAGAACATCCATATCATTTCTGATGAAATATTTGCTGGGTCTATGTATGGAAGTGAGGAGTTTGTAAGCATGGCAGAAATTATTGACACCGAAGATTTTGACAAGAACAGGGTTCACATAATATATGGGCTATCAAAAGACCTATCTATTCCAGGGTTTAGGATTGGGGTTTTATATTCATATAATGAGAGTGTTTTGACTGCTGCTAAAAGGTTAACAAGATTTTCTCCCATTTCTTCTCTTACCCAGCGGCTTATTATTTCGATGCTCTCGGATGCAAGATTCATTCAGGAATACCTAGAGATCAACAAAAGGAGGATACAAAGTATATCTGAATTATTTGTGGCTGGTTTGCAACAATTAGGAATCAAGTGTGCAAAGAGCAGTGCTGGCTTATACTGTTGGGCTGATATGAGTGGATTAATTCGCTCTTATGGTGAGAAAGGGGAACTCGAGTTATGGGATAAGCTGTTGAACATTGCCAAGATCAATGTGACTCCTGGGTCAGCCTGCCACTGCATAGAACCTGGATGGTTCAGATGTTGTTTTACTACTTTGACTCCAGAGGATGTTCCTGTTGTTATAGACCGAATCCGAAAAGTTACTGAAACAATTAAGTCGTCCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

509

Amino Acids

56.45

Weight (kDa)

7.94

Isoelectric Point (pI)

54.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51690 AT5G51690
fragaria_vesca FvH4_7g28260
malus_domestica MD01G1186400.v1.1
prunus_persica Prupe.2G283100_v2.0.a1
pyrus_communis pycom01g19800
rosa_chinensis RchiOBHm_Chr1g0375231
rosa_laevigata RLG00000026677
rosa_multiflora Rmu_co8467605.1_g000001
rosa_roxburghii Rroxscaffold_4G00282920
rosa_rugosa Rorug01G0389200
rosa_samantha Rh1AG399900 Rh1BG364500 Rh1CG377500 Rh1DG395100
rosa_wichuraiana Rw1G035500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1399
AccII CGCG 1 cut(s) 196
AciI CCGC 4 cut(s) 196, 229, 470, 1147
AclWI GGATC 2 cut(s) 213, 226
AcsI RAATTY 1 cut(s) 412
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 3 cut(s) 234, 320, 476
AfiI CCNNNNNNNGG 5 cut(s) 23, 195, 228, 372, 488
AgsI TTSAA 2 cut(s) 767, 1372
AjnI CCWGG 4 cut(s) 667, 1048, 1396, 1423
AluBI AGCT 7 cut(s) 97, 178, 355, 502, 615, 709, 1366
AluI AGCT 7 cut(s) 97, 178, 355, 502, 615, 709, 1366
Alw21I GWGCWC 3 cut(s) 79, 180, 504
Alw26I GTCTC 1 cut(s) 534
AlwI GGATC 2 cut(s) 213, 226
Ama87I CYCGRG 3 cut(s) 246, 356, 1349
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 2 cut(s) 729, 1100
ApoI RAATTY 1 cut(s) 412
AseI ATTAAT 1 cut(s) 1319
AspS9I GGNCC 2 cut(s) 198, 313
AsuC2I CCSGG 2 cut(s) 247, 248
AsuHPI GGTGA 2 cut(s) 458, 1346
AsuII TTCGAA 1 cut(s) 833
AvaI CYCGRG 3 cut(s) 246, 356, 1349
AvaII GGWCC 2 cut(s) 198, 313
BamHI GGATCC 1 cut(s) 218
BanII GRGCYC 2 cut(s) 180, 504
BbsI GAAGAC 1 cut(s) 444
Bbv12I GWGCWC 3 cut(s) 79, 180, 504
BbvI GCAGC 2 cut(s) 741, 1087
BccI CCATC 6 cut(s) 139, 157, 482, 571, 684, 1422
BceAI ACGGC 1 cut(s) 466
BciT130I CCWGG 4 cut(s) 669, 1050, 1398, 1425
BciVI GTATCC 2 cut(s) 250, 1209
BcnI CCSGG 2 cut(s) 247, 248
BcoDI GTCTC 1 cut(s) 534
BfaI CTAG 2 cut(s) 786, 1196
BfmI CTRYAG 1 cut(s) 597
BfuI GTATCC 2 cut(s) 250, 1209
BisI GCNGC 4 cut(s) 471, 730, 1101, 1148
BlpI GCTNAGC 1 cut(s) 622
BlsI GCNGC 4 cut(s) 472, 731, 1102, 1149
Bme1390I CCNGG 6 cut(s) 247, 248, 669, 1050, 1398, 1425
Bme18I GGWCC 2 cut(s) 198, 313
BmeT110I CYCGRG 3 cut(s) 246, 356, 1349
BmgT120I GGNCC 2 cut(s) 198, 313
BmiI GGNNCC 2 cut(s) 220, 315
BmrFI CCNGG 6 cut(s) 247, 248, 669, 1050, 1398, 1425
BmsI GCATC 2 cut(s) 1152, 1162
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 3 cut(s) 77, 164, 1443
Bpu1102I GCTNAGC 1 cut(s) 622
Bpu14I TTCGAA 1 cut(s) 833
BpuMI CCSGG 2 cut(s) 247, 248
BsaHI GRCGYC 1 cut(s) 27
BsaJI CCNNGG 9 cut(s) 22, 34, 55, 214, 222, 246, 631, 1049, 1397
BsaXI ACNNNNNCTCC 8 cut(s) 104, 134, 302, 332, 789, 819, 1441, 1471
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 195, 228, 372, 488
Bse1I ACTGG 1 cut(s) 1521
Bse3DI GCAATG 1 cut(s) 1374
BseBI CCWGG 4 cut(s) 669, 1050, 1398, 1425
BseDI CCNNGG 9 cut(s) 22, 34, 55, 214, 222, 246, 631, 1049, 1397
BseGI GGATG 6 cut(s) 131, 149, 903, 1177, 1433, 1471
BseLI CCNNNNNNNGG 5 cut(s) 23, 195, 228, 372, 488
BseMI GCAATG 1 cut(s) 1374
BseMII CTCAG 2 cut(s) 489, 545
BseNI ACTGG 1 cut(s) 1521
BseRI GAGGAG 4 cut(s) 148, 151, 810, 968
BseXI GCAGC 2 cut(s) 741, 1087
BseYI CCCAGC 2 cut(s) 932, 1143
BsgI GTGCAG 2 cut(s) 827, 892
Bsh1236I CGCG 1 cut(s) 196
BshFI GGCC 1 cut(s) 48
BsiHKAI GWGCWC 3 cut(s) 79, 180, 504
BsiHKCI CYCGRG 3 cut(s) 246, 356, 1349
BsiSI CCGG 1 cut(s) 247
BsiWI CGTACG 1 cut(s) 318
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 5 cut(s) 23, 195, 228, 372, 488
BsmAI GTCTC 1 cut(s) 534
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 1 cut(s) 48
BsoBI CYCGRG 3 cut(s) 246, 356, 1349
Bsp119I TTCGAA 1 cut(s) 833
Bsp1286I GDGCHC 3 cut(s) 79, 180, 504
Bsp143I GATC 3 cut(s) 218, 1200, 1383
Bsp1720I GCTNAGC 1 cut(s) 622
Bsp19I CCATGG 2 cut(s) 222, 631
BspACI CCGC 4 cut(s) 196, 229, 470, 1147
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 2 cut(s) 490, 544
BspFNI CGCG 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 220, 315
BspMAI CTGCAG 1 cut(s) 601
BspPI GGATC 2 cut(s) 213, 226
BspT104I TTCGAA 1 cut(s) 833
BsrDI GCAATG 1 cut(s) 1374
BsrI ACTGG 1 cut(s) 1521
BssECI CCNNGG 9 cut(s) 22, 34, 55, 214, 222, 246, 631, 1049, 1397
BssMI GATC 3 cut(s) 218, 1200, 1383
BssNI GRCGYC 1 cut(s) 27
BssT1I CCWWGG 4 cut(s) 22, 34, 222, 631
Bst2UI CCWGG 4 cut(s) 669, 1050, 1398, 1425
Bst4CI ACNGT 6 cut(s) 116, 443, 725, 760, 870, 1298
Bst6I CTCTTC 1 cut(s) 86
BstACI GRCGYC 1 cut(s) 27
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 833
BstC8I GCNNGC 3 cut(s) 696, 1288, 1408
BstDEI CTNAG 3 cut(s) 498, 531, 622
BstDSI CCRYGG 3 cut(s) 214, 222, 631
BstF5I GGATG 6 cut(s) 131, 149, 903, 1177, 1433, 1471
BstFNI CGCG 1 cut(s) 196
BstKTI GATC 3 cut(s) 221, 1203, 1386
BstMAI GTCTC 1 cut(s) 534
BstMBI GATC 3 cut(s) 218, 1200, 1383
BstMWI GCNNNNNNNGC 3 cut(s) 45, 596, 1287
BstNI CCWGG 4 cut(s) 669, 1050, 1398, 1425
BstSCI CCNGG 6 cut(s) 245, 246, 667, 1048, 1396, 1423
BstSFI CTRYAG 1 cut(s) 597
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 2 cut(s) 741, 1087
BstV2I GAAGAC 1 cut(s) 444
BstX2I RGATCY 1 cut(s) 218
BstYI RGATCY 1 cut(s) 218
BsuI GTATCC 2 cut(s) 250, 1209
BsuRI GGCC 1 cut(s) 48
BtgI CCRYGG 3 cut(s) 214, 222, 631
BtgZI GCGATG 1 cut(s) 154
BtsCI GGATG 6 cut(s) 131, 149, 903, 1177, 1433, 1471
BtsI GCAGTG 2 cut(s) 1288, 1411
BtsIMutI CAGTG 6 cut(s) 555, 721, 732, 866, 1288, 1411
Cac8I GCNNGC 3 cut(s) 696, 1288, 1408
Cfr13I GGNCC 2 cut(s) 198, 313
Cfr9I CCCGGG 1 cut(s) 246
CseI GACGC 2 cut(s) 16, 427
Csp6I GTAC 3 cut(s) 233, 319, 475
CviAII CATG 4 cut(s) 223, 526, 632, 967
CviQI GTAC 3 cut(s) 233, 319, 475
DdeI CTNAG 3 cut(s) 498, 531, 622
DpnI GATC 3 cut(s) 220, 1202, 1385
DpnII GATC 3 cut(s) 218, 1200, 1383
DraI TTTAAA 1 cut(s) 750
DrdI GACNNNNNNGTC 1 cut(s) 1399
DseDI GACNNNNNNGTC 1 cut(s) 1399
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Ecl136II GAGCTC 2 cut(s) 178, 502
Eco130I CCWWGG 4 cut(s) 22, 34, 222, 631
Eco24I GRGCYC 2 cut(s) 180, 504
Eco47I GGWCC 2 cut(s) 198, 313
Eco53kI GAGCTC 2 cut(s) 178, 502
Eco88I CYCGRG 3 cut(s) 246, 356, 1349
EcoICRI GAGCTC 2 cut(s) 178, 502
EcoO109I RGGNCCY 1 cut(s) 313
EcoRII CCWGG 4 cut(s) 667, 1048, 1396, 1423
EcoT14I CCWWGG 4 cut(s) 22, 34, 222, 631
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 2 cut(s) 180, 504
ErhI CCWWGG 4 cut(s) 22, 34, 222, 631
FaeI CATG 4 cut(s) 226, 529, 635, 970
FaqI GGGAC 1 cut(s) 102
FatI CATG 4 cut(s) 222, 525, 631, 966
FauI CCCGC 1 cut(s) 222
Fnu4HI GCNGC 4 cut(s) 471, 730, 1101, 1148
FokI GGATG 6 cut(s) 118, 136, 890, 1184, 1440, 1478
FriOI GRGCYC 2 cut(s) 180, 504
Fsp4HI GCNGC 4 cut(s) 471, 730, 1101, 1148
FspBI CTAG 2 cut(s) 786, 1196
GluI GCNGC 4 cut(s) 471, 730, 1101, 1148
GsaI CCCAGC 2 cut(s) 936, 1147
GsuI CTGGAG 3 cut(s) 77, 164, 1443
HaeIII GGCC 1 cut(s) 48
HapII CCGG 1 cut(s) 247
HgaI GACGC 2 cut(s) 16, 427
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 4 cut(s) 226, 529, 635, 970
HincII GTYRAC 2 cut(s) 579, 1113
HindII GTYRAC 2 cut(s) 579, 1113
HindIII AAGCTT 1 cut(s) 613
HinfI GANTC 8 cut(s) 275, 712, 835, 1179, 1263, 1393, 1456, 1488
HpaI GTTAAC 2 cut(s) 579, 1113
HpaII CCGG 1 cut(s) 247
HphI GGTGA 2 cut(s) 458, 1346
Hpy166II GTNNAC 6 cut(s) 210, 331, 579, 721, 1012, 1113
Hpy188I TCNGA 8 cut(s) 339, 367, 434, 919, 1171, 1231, 1436, 1493
Hpy188III TCNNGA 4 cut(s) 181, 764, 1187, 1460
Hpy8I GTNNAC 6 cut(s) 210, 331, 579, 721, 1012, 1113
Hpy99I CGWCG 2 cut(s) 32, 196
HpyAV CCTTC 2 cut(s) 153, 840
HpyCH4III ACNGT 6 cut(s) 116, 443, 725, 760, 870, 1298
HpyCH4V TGCA 9 cut(s) 590, 599, 641, 808, 873, 1175, 1252, 1274, 1416
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 596, 1287
HpyF3I CTNAG 3 cut(s) 498, 531, 622
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 4 cut(s) 226, 529, 635, 970
KspAI GTTAAC 2 cut(s) 579, 1113
Kzo9I GATC 3 cut(s) 218, 1200, 1383
LmnI GCTCC 2 cut(s) 183, 706
Lsp1109I GCAGC 2 cut(s) 741, 1087
LweI GCATC 2 cut(s) 1152, 1162
MaeI CTAG 2 cut(s) 786, 1196
MaeIII GTNAC 2 cut(s) 1390, 1498
MalI GATC 3 cut(s) 220, 1202, 1385
MboI GATC 3 cut(s) 218, 1200, 1383
MboII GAAGA 5 cut(s) 73, 449, 910, 1001, 1125
MflI RGATCY 1 cut(s) 218
MhlI GDGCHC 3 cut(s) 79, 180, 504
MluCI AATT 8 cut(s) 412, 426, 847, 975, 1232, 1256, 1320, 1509
MlyI GAGTC 3 cut(s) 269, 1387, 1450
MmeI TCCRAC 3 cut(s) 225, 236, 276
Mph1103I ATGCAT 1 cut(s) 643
MseI TTAA 6 cut(s) 578, 611, 749, 1112, 1319, 1512
MslI CAYNNNNRTG 1 cut(s) 636
MspA1I CMGCKG 1 cut(s) 1147
MspI CCGG 1 cut(s) 247
MspR9I CCNGG 6 cut(s) 247, 248, 669, 1050, 1398, 1425
MvaI CCWGG 4 cut(s) 669, 1050, 1398, 1425
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 3 cut(s) 45, 596, 1287
NciI CCSGG 2 cut(s) 247, 248
NcoI CCATGG 2 cut(s) 222, 631
NdeII GATC 3 cut(s) 218, 1200, 1383
NlaIII CATG 4 cut(s) 226, 529, 635, 970
NlaIV GGNNCC 2 cut(s) 220, 315
NmeAIII GCCGAG 2 cut(s) 36, 199
NmuCI GTSAC 1 cut(s) 1390
NsiI ATGCAT 1 cut(s) 643
NspV TTCGAA 1 cut(s) 833
PaeR7I CTCGAG 1 cut(s) 1349
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PfeI GAWTC 5 cut(s) 712, 835, 1179, 1263, 1488
Pfl23II CGTACG 1 cut(s) 318
PflFI GACNNNGTC 1 cut(s) 114
PkrI GCNGC 4 cut(s) 472, 731, 1102, 1149
PleI GAGTC 3 cut(s) 269, 1387, 1450
PpsI GAGTC 3 cut(s) 269, 1387, 1450
PpuMI RGGWCCY 1 cut(s) 313
PshBI ATTAAT 1 cut(s) 1319
Psp124BI GAGCTC 2 cut(s) 180, 504
Psp5II RGGWCCY 1 cut(s) 313
Psp6I CCWGG 4 cut(s) 667, 1048, 1396, 1423
PspFI CCCAGC 2 cut(s) 932, 1143
PspGI CCWGG 4 cut(s) 667, 1048, 1396, 1423
PspLI CGTACG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 220, 315
PspPI GGNCC 2 cut(s) 198, 313
PspPPI RGGWCCY 1 cut(s) 313
PspXI VCTCGAGB 1 cut(s) 1349
PsrI GAACNNNNNNTAC 2 cut(s) 467, 499
PstI CTGCAG 1 cut(s) 601
PsuI RGATCY 1 cut(s) 218
PsyI GACNNNGTC 1 cut(s) 114
RsaI GTAC 3 cut(s) 234, 320, 476
RsaNI GTAC 3 cut(s) 233, 319, 475
RseI CAYNNNNRTG 1 cut(s) 636
SacI GAGCTC 2 cut(s) 180, 504
SaqAI TTAA 6 cut(s) 578, 611, 749, 1112, 1319, 1512
SatI GCNGC 4 cut(s) 471, 730, 1101, 1148
Sau3AI GATC 3 cut(s) 218, 1200, 1383
Sau96I GGNCC 2 cut(s) 198, 313
SchI GAGTC 3 cut(s) 269, 1387, 1450
ScrFI CCNGG 6 cut(s) 247, 248, 669, 1050, 1398, 1425
SduI GDGCHC 3 cut(s) 79, 180, 504
SfaNI GCATC 2 cut(s) 1152, 1162
SfcI CTRYAG 1 cut(s) 597
Sfr274I CTCGAG 1 cut(s) 1349
SfuI TTCGAA 1 cut(s) 833
SinI GGWCC 2 cut(s) 198, 313
SlaI CTCGAG 1 cut(s) 1349
SmaI CCCGGG 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 636
SmlI CTYRAG 1 cut(s) 1349
SmoI CTYRAG 1 cut(s) 1349
Sse9I AATT 8 cut(s) 412, 426, 847, 975, 1232, 1256, 1320, 1509
SsiI CCGC 4 cut(s) 196, 229, 470, 1147
SspI AATATT 1 cut(s) 927
SspMI CTAG 2 cut(s) 786, 1196
SstI GAGCTC 2 cut(s) 180, 504
StyD4I CCNGG 6 cut(s) 245, 246, 667, 1048, 1396, 1423
StyI CCWWGG 4 cut(s) 22, 34, 222, 631
TaaI ACNGT 6 cut(s) 116, 443, 725, 760, 870, 1298
TaqI TCGA 5 cut(s) 164, 486, 833, 1160, 1350
TaqII GACCGA 1 cut(s) 1500
TasI AATT 8 cut(s) 412, 426, 847, 975, 1232, 1256, 1320, 1509
TauI GCSGC 2 cut(s) 473, 1150
TfiI GAWTC 5 cut(s) 712, 835, 1179, 1263, 1488
Tru1I TTAA 6 cut(s) 578, 611, 749, 1112, 1319, 1512
Tru9I TTAA 6 cut(s) 578, 611, 749, 1112, 1319, 1512
TscAI CASTG 6 cut(s) 555, 728, 739, 873, 1288, 1418
TseFI GTSAC 1 cut(s) 1390
TseI GCWGC 2 cut(s) 729, 1100
Tsp45I GTSAC 1 cut(s) 1390
TspDTI ATGAA 4 cut(s) 514, 936, 1065, 1171
TspGWI ACGGA 1 cut(s) 422
TspMI CCCGGG 1 cut(s) 246
TspRI CASTG 6 cut(s) 555, 728, 739, 873, 1288, 1418
Tth111I GACNNNGTC 1 cut(s) 114
VpaK11BI GGWCC 2 cut(s) 198, 313
VspI ATTAAT 1 cut(s) 1319
XapI RAATTY 1 cut(s) 412
XcmI CCANNNNNNNNNTGG 2 cut(s) 41, 485
XhoI CTCGAG 1 cut(s) 1349
XmaI CCCGGG 1 cut(s) 246
XspI CTAG 2 cut(s) 786, 1196
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.