AT5G52280

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007167
Physical Location & Seq
Forward (+)
21226721 .. 21230378
3658 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52280.1

Sequence Viewer

Length: 2562 bp
ATGTTTAAGTCATGGAGGAACGACAAGAACAAGATCAAGGCTGTGTTCAAACTTCAGTTTCAGGCAACTCAGGTGCCGAAGCTGAAGAAGACTGCTTTGATGATCTCATTGGTACCTGATGATGTTGGGAAGCCAACATTTAAGCTTGAGAAATCTGAGGTTAAAGAAGGGATCTGTTCATGGGAGAATCCAATTTATGTGTCAGTGAAGCTAATCAAAGAACCAAAAACTGGGATTGTTCGTGAGAAGATTTACCATTTCGTTGTTGCAACAGGTTCTTCGAAATCGGGTTTTCTAGGAGAAGCTTCCATTGATTTTGCAGATTTTCTGACAGAAGCAGATCCATTAACTGTCTCCTTACCTCTCAAATTTGCCAACTCTGGTGCTGTCTTAAATGTGACAATCCATAAGATACAAGGAGCGAGTGATTTAAAATTTATTGAGGAAAATAAAGATCAAACACTTAGCAAAGAAGACAGCTTCAAAAGTCTACAAAGCAATGATGACTTAGAAGGATATAACCAAGATGAAAGGAGCTTAGATGTGAACACGGCTAAGAATGCTGGCCTAGGAGGTTCCTTTGATTCCATTGGTGAATCTGGATGGATAGATGATGGGAATGCACGGTTACCTCAACGACATAACTCAGTTCCTGCAACAAGAAACGGGCATCGAAGATCAAACACAGACTGGTCAGCTAGTTCGACATCAGATGAAAGTTACATTGAGTCAAGAAATAGTCCTGAGAACAGTTTCCAAAGAGGTTTCTCTAGTGTTACTGAATCGAGTGACCCTATTGAAAGGCTCAAGATGGAATTGGAAGCTTTGAGGAGGCAATCGGAGTTATCTGAGTTAGAAAAACAGTCTTTAAGGAAACAAGCCATAAAGGAGAGTAAACGGATACAGGAACTGTCTAAGGAAGTTAGTTGTCTCAAGGGTGAACGTGATGGAGCTATGGAAGAGTGTGAGAAGCTCAGGTTGCAGAATAGTAGAGATGAGGCTGATGCTGAAAGCAGATTGAGATGCATCAGCGAAGATTCGAGTAATATGATTGAGGAGATTAGAGATGAACTGAGTTGTGAGAAGGATTTGACGAGTAACCTTAAGTTGCAGTTGCAGAGGACACAAGAGTCAAACTCTAATTTGATTCTTGCTGTGAGGGATCTCAATGAGATGTTGGAGCAGAAGAACAATGAGATATCTTCTCTGAATAGCTTATTAGAGGAGGCTAAGAAGCTTGAAGAGCATAAAGGAATGGATTCTGGAAACAATGAGATAGATACGCTAAAGCAACAGATCGAAGATTTAGATTGGGAGTTAGACTCTTACAAGAAAAAGAACGAAGAACAAGAGATACTATTAGATGAGCTTACTCAGGAATATGAGTCCTTGAAAGAGGAAAATTACAAAAACGTTTCCTCAAAATTAGAGCAGCAAGAATGCTCAAATGCAGAAGACGAATACTTGGATTCCAAGGATATTATAGACGAATTGAAATCTCAGATAGAGATATTGGAAGGCAAACTGAAGCAGCAATCGTTGGAGTATTCTGAGTGCTTGATTACAGTTAATGAACTTGAGAGTCAGGTGAAAGAGTTAAAGAAAGAACTGGAGGATCAGGCACAGGCTTATGATGAAGATATCGACACAATGATGCGGGAAAAAACTGAACAAGAGCAAAGAGCCATCAAAGCAGAGGAGAACCTGAGGAAAACAAGATGGAATAACGCTATAACTGCAGAGCGGCTTCAAGAGAAATGCAAGAGGCTTTCTCTAGAAATGGAATCGAAGCTGAGCGAGCATGAGAATCTGACGAAAAAAACATTGGCTGAAGCCAACAACCTTCGTCTGCAAAACAAAACTCTTGAGGAAATGCAAGAGAAAACACATACCGAGATTACACAAGAGAAAGAACAAAGGAAGCACGTGGAAGAGAAGAACAAAGCTTTGTCAATGAAAGTCCAGATGCTTGAAAGTGAGGTCCTGAAGCTCACAAAGCTGAGAGATGAGTCGAGTGCAGCAGCTACTGAAACCGAGAAGATAATACAAGAGTGGAGGAAAGAAAGAGATGAATTTGAGAGAAAACTTTCTTTGGCTAAAGAAGTAGCCAAGACAGCGCAGAAAGAGTTAACTCTCACTAAGTCGTCAAATGATGATAAAGAGACAAGGCTCAGGAATTTGAAGACAGAAGTAGAAGGTCTAAGCCTACAGTACAGTGAATTACAAAACAGCTTCGTTCAAGAAAAAATGGAGAATGATGAACTGAGAAAACAAGTATCAAACCTGAAAGTCGATATTCGGAGAAAAGAAGAAGAAATGACTAAAATCCTAGATGCAAGGATGGAAGCAAGGTCACAAGAAAACGGACACAAAGAGGAGAATCTATCAAAGCTATCAGACGAATTGGCGTATTGTAAGAACAAAAACAGTTCAATGGAGAGAGAGTTGAAAGAAATGGAAGAGAGATATTCAGAGATAAGCCTGAGATTTGCAGAGGTAGAAGGAGAAAGACAACAACTTGTGATGGCTGTCAGAAACCTCAAGAACGGTAAGAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

853

Amino Acids

98.5

Weight (kDa)

5.07

Isoelectric Point (pI)

53.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 9 - 143 6.5e-23 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1129
Acc65I GGTACC 1 cut(s) 112
AccB1I GGYRCC 2 cut(s) 73, 112
AccB7I CCANNNNNTGG 1 cut(s) 230
AccBSI CCGCTC 1 cut(s) 1744
AccI GTMKAC 1 cut(s) 490
AciI CCGC 2 cut(s) 1657, 1744
AclI AACGTT 1 cut(s) 1413
AclWI GGATC 4 cut(s) 179, 335, 1170, 1623
AcsI RAATTY 4 cut(s) 368, 434, 2072, 2176
AcuI CTGAAG 5 cut(s) 38, 104, 1547, 1851, 2006
AcvI CACGTG 1 cut(s) 1927
AfaI GTAC 2 cut(s) 114, 2213
AfiI CCNNNNNNNGG 1 cut(s) 230
AflII CTTAAG 1 cut(s) 1103
AjuI GAANNNNNNNTTGG 2 cut(s) 1808, 1840
Alw26I GTCTC 3 cut(s) 358, 935, 2156
AlwI GGATC 4 cut(s) 179, 335, 1170, 1623
AlwNI CAGNNNCTG 3 cut(s) 653, 910, 2027
AoxI GGCC 1 cut(s) 565
ApeKI GCWGC 4 cut(s) 1432, 1531, 2018, 2021
ApoI RAATTY 4 cut(s) 368, 434, 2072, 2176
Asp700I GAANNNNTTC 3 cut(s) 752, 1258, 2086
Asp718I GGTACC 1 cut(s) 112
AspA2I CCTAGG 1 cut(s) 568
AspLEI GCGC 1 cut(s) 2119
AspS9I GGNCC 1 cut(s) 1981
AsuHPI GGTGA 3 cut(s) 605, 950, 1600
AsuII TTCGAA 1 cut(s) 281
AvaII GGWCC 1 cut(s) 1981
AvrII CCTAGG 1 cut(s) 568
AxyI CCTNAGG 1 cut(s) 1706
BanI GGYRCC 2 cut(s) 73, 112
BbrPI CACGTG 1 cut(s) 1927
BbsI GAAGAC 4 cut(s) 95, 480, 1461, 2189
BbvI GCAGC 4 cut(s) 1444, 1543, 2030, 2033
BccI CCATC 8 cut(s) 597, 608, 805, 941, 1694, 1713, 2335, 2518
BceAI ACGGC 1 cut(s) 567
BciVI GTATCC 1 cut(s) 894
BcoDI GTCTC 3 cut(s) 358, 935, 2156
BfaI CTAG 7 cut(s) 296, 569, 699, 771, 1775, 2330, 2560
BfmI CTRYAG 2 cut(s) 1737, 2207
BfrI CTTAAG 1 cut(s) 1103
BfuI GTATCC 1 cut(s) 894
BisI GCNGC 5 cut(s) 1433, 1532, 1745, 2019, 2022
BlnI CCTAGG 1 cut(s) 568
BlpI GCTNAGC 1 cut(s) 1793
BlsI GCNGC 5 cut(s) 1434, 1533, 1746, 2020, 2023
Bme18I GGWCC 1 cut(s) 1981
BmgT120I GGNCC 1 cut(s) 1981
BmiI GGNNCC 3 cut(s) 75, 114, 577
BmrI ACTGGG 1 cut(s) 240
BmsI GCATC 7 cut(s) 679, 994, 1013, 1035, 1644, 1956, 2323
BmuI ACTGGG 1 cut(s) 240
BpiI GAAGAC 4 cut(s) 95, 480, 1461, 2189
BplI GAGNNNNNCTC 6 cut(s) 1121, 1153, 1307, 1339, 1756, 1788
BpmI CTGGAG 1 cut(s) 1631
Bpu10I CCTNAGC 2 cut(s) 974, 2171
Bpu1102I GCTNAGC 1 cut(s) 1793
Bpu14I TTCGAA 1 cut(s) 281
BpuEI CTTGAG 6 cut(s) 167, 791, 917, 1598, 1886, 2525
BsaAI YACGTR 1 cut(s) 1927
BsaJI CCNNGG 2 cut(s) 568, 1473
BsaXI ACNNNNNCTCC 2 cut(s) 2429, 2459
Bsc4I CCNNNNNNNGG 1 cut(s) 230
Bse1I ACTGG 3 cut(s) 235, 695, 1614
Bse21I CCTNAGG 1 cut(s) 1706
Bse3DI GCAATG 1 cut(s) 505
BseDI CCNNGG 2 cut(s) 568, 1473
BseGI GGATG 2 cut(s) 608, 2346
BseLI CCNNNNNNNGG 1 cut(s) 230
BseMI GCAATG 1 cut(s) 505
BseNI ACTGG 3 cut(s) 235, 695, 1614
BseRI GAGGAG 5 cut(s) 844, 1070, 1238, 1712, 2390
BseXI GCAGC 4 cut(s) 1444, 1543, 2030, 2033
BsgI GTGCAG 1 cut(s) 2037
BshFI GGCC 1 cut(s) 567
BshNI GGYRCC 2 cut(s) 73, 112
BslI CCNNNNNNNGG 1 cut(s) 230
BsmAI GTCTC 3 cut(s) 358, 935, 2156
BsmI GAATGC 3 cut(s) 565, 625, 1445
BsnI GGCC 1 cut(s) 567
Bsp119I TTCGAA 1 cut(s) 281
Bsp143I GATC 9 cut(s) 33, 102, 171, 340, 454, 677, 1162, 1296, 1615
Bsp1720I GCTNAGC 1 cut(s) 1793
BspACI CCGC 2 cut(s) 1657, 1744
BspANI GGCC 1 cut(s) 567
BspLI GGNNCC 3 cut(s) 75, 114, 577
BspMAI CTGCAG 1 cut(s) 1741
BspPI GGATC 4 cut(s) 179, 335, 1170, 1623
BspQI GCTCTTC 1 cut(s) 1236
BspT104I TTCGAA 1 cut(s) 281
BspT107I GGYRCC 2 cut(s) 73, 112
BspTI CTTAAG 1 cut(s) 1103
BsrBI CCGCTC 1 cut(s) 1744
BsrDI GCAATG 1 cut(s) 505
BsrI ACTGG 3 cut(s) 235, 695, 1614
BssECI CCNNGG 2 cut(s) 568, 1473
BssMI GATC 9 cut(s) 33, 102, 171, 340, 454, 677, 1162, 1296, 1615
BssT1I CCWWGG 2 cut(s) 568, 1473
Bst6I CTCTTC 4 cut(s) 954, 1236, 1926, 2454
BstAFI CTTAAG 1 cut(s) 1103
BstBAI YACGTR 1 cut(s) 1927
BstBI TTCGAA 1 cut(s) 281
BstC8I GCNNGC 2 cut(s) 565, 1799
BstEII GGTNACC 1 cut(s) 627
BstF5I GGATG 2 cut(s) 608, 2346
BstHHI GCGC 1 cut(s) 2119
BstKTI GATC 9 cut(s) 36, 105, 174, 343, 457, 680, 1165, 1299, 1618
BstMAI GTCTC 3 cut(s) 358, 935, 2156
BstMBI GATC 9 cut(s) 33, 102, 171, 340, 454, 677, 1162, 1296, 1615
BstMWI GCNNNNNNNGC 8 cut(s) 560, 979, 1243, 1691, 1736, 1798, 1996, 2114
BstPI GGTNACC 1 cut(s) 627
BstSFI CTRYAG 2 cut(s) 1737, 2207
BstV1I GCAGC 4 cut(s) 1444, 1543, 2030, 2033
BstV2I GAAGAC 4 cut(s) 95, 480, 1461, 2189
BstX2I RGATCY 3 cut(s) 171, 340, 1162
BstYI RGATCY 3 cut(s) 171, 340, 1162
Bsu36I CCTNAGG 1 cut(s) 1706
BsuI GTATCC 1 cut(s) 894
BsuRI GGCC 1 cut(s) 567
BtsCI GGATG 2 cut(s) 608, 2346
BtsIMutI CAGTG 2 cut(s) 210, 2221
Cac8I GCNNGC 2 cut(s) 565, 1799
CaiI CAGNNNCTG 3 cut(s) 653, 910, 2027
CfoI GCGC 1 cut(s) 2119
Cfr13I GGNCC 1 cut(s) 1981
Csp6I GTAC 2 cut(s) 113, 2212
CviAII CATG 3 cut(s) 12, 180, 1802
CviQI GTAC 2 cut(s) 113, 2212
DpnI GATC 9 cut(s) 35, 104, 173, 342, 456, 679, 1164, 1298, 1617
DpnII GATC 9 cut(s) 33, 102, 171, 340, 454, 677, 1162, 1296, 1615
DraI TTTAAA 1 cut(s) 432
DrdI GACNNNNNNGTC 1 cut(s) 1129
DseDI GACNNNNNNGTC 1 cut(s) 1129
Eam1104I CTCTTC 4 cut(s) 954, 1236, 1926, 2454
EarI CTCTTC 4 cut(s) 954, 1236, 1926, 2454
Eco130I CCWWGG 2 cut(s) 568, 1473
Eco32I GATATC 2 cut(s) 1200, 1642
Eco47I GGWCC 1 cut(s) 1981
Eco57I CTGAAG 5 cut(s) 38, 104, 1547, 1851, 2006
Eco72I CACGTG 1 cut(s) 1927
Eco81I CCTNAGG 1 cut(s) 1706
Eco91I GGTNACC 1 cut(s) 627
EcoO109I RGGNCCY 1 cut(s) 1981
EcoO65I GGTNACC 1 cut(s) 627
EcoRV GATATC 2 cut(s) 1200, 1642
EcoT14I CCWWGG 2 cut(s) 568, 1473
EcoT22I ATGCAT 1 cut(s) 1028
ErhI CCWWGG 2 cut(s) 568, 1473
FaeI CATG 3 cut(s) 15, 183, 1805
FatI CATG 3 cut(s) 11, 179, 1801
FauI CCCGC 1 cut(s) 1650
FblI GTMKAC 1 cut(s) 490
Fnu4HI GCNGC 5 cut(s) 1433, 1532, 1745, 2019, 2022
FokI GGATG 2 cut(s) 615, 2353
Fsp4HI GCNGC 5 cut(s) 1433, 1532, 1745, 2019, 2022
FspBI CTAG 7 cut(s) 296, 569, 699, 771, 1775, 2330, 2560
GlaI GCGC 1 cut(s) 2118
GluI GCNGC 5 cut(s) 1433, 1532, 1745, 2019, 2022
GsuI CTGGAG 1 cut(s) 1631
HaeIII GGCC 1 cut(s) 567
HhaI GCGC 1 cut(s) 2119
Hin1II CATG 3 cut(s) 15, 183, 1805
Hin6I GCGC 1 cut(s) 2117
HinP1I GCGC 1 cut(s) 2117
HincII GTYRAC 1 cut(s) 2130
HindII GTYRAC 1 cut(s) 2130
HindIII AAGCTT 5 cut(s) 143, 303, 822, 1235, 1944
HpaI GTTAAC 1 cut(s) 2130
HphI GGTGA 3 cut(s) 605, 950, 1600
Hpy166II GTNNAC 5 cut(s) 491, 547, 896, 941, 2130
Hpy8I GTNNAC 5 cut(s) 491, 547, 896, 941, 2130
HpyAV CCTTC 7 cut(s) 161, 506, 1078, 1511, 1853, 2189, 2495
HpyCH4IV ACGT 3 cut(s) 943, 1413, 1926
HpyF10VI GCNNNNNNNGC 8 cut(s) 560, 979, 1243, 1691, 1736, 1798, 1996, 2114
HpySE526I ACGT 3 cut(s) 943, 1413, 1926
Hsp92II CATG 3 cut(s) 15, 183, 1805
HspAI GCGC 1 cut(s) 2117
KpnI GGTACC 1 cut(s) 116
KspAI GTTAAC 1 cut(s) 2130
Kzo9I GATC 9 cut(s) 33, 102, 171, 340, 454, 677, 1162, 1296, 1615
LguI GCTCTTC 1 cut(s) 1236
LmnI GCTCC 4 cut(s) 419, 534, 950, 1180
Lsp1109I GCAGC 4 cut(s) 1444, 1543, 2030, 2033
LweI GCATC 7 cut(s) 679, 994, 1013, 1035, 1644, 1956, 2323
MaeI CTAG 7 cut(s) 296, 569, 699, 771, 1775, 2330, 2560
MaeII ACGT 3 cut(s) 943, 1413, 1926
MaeIII GTNAC 7 cut(s) 397, 627, 719, 775, 788, 1097, 2352
MalI GATC 9 cut(s) 35, 104, 173, 342, 456, 679, 1164, 1298, 1617
MbiI CCGCTC 1 cut(s) 1744
MboI GATC 9 cut(s) 33, 102, 171, 340, 454, 677, 1162, 1296, 1615
MflI RGATCY 3 cut(s) 171, 340, 1162
MlyI GAGTC 6 cut(s) 737, 1139, 1316, 1394, 1591, 2018
MmeI TCCRAC 2 cut(s) 1158, 1521
Mph1103I ATGCAT 1 cut(s) 1028
MroXI GAANNNNTTC 3 cut(s) 752, 1258, 2086
MslI CAYNNNNRTG 1 cut(s) 1652
MspCI CTTAAG 1 cut(s) 1103
Mva1269I GAATGC 3 cut(s) 565, 625, 1445
MwoI GCNNNNNNNGC 8 cut(s) 560, 979, 1243, 1691, 1736, 1798, 1996, 2114
NdeII GATC 9 cut(s) 33, 102, 171, 340, 454, 677, 1162, 1296, 1615
NlaIII CATG 3 cut(s) 15, 183, 1805
NlaIV GGNNCC 3 cut(s) 75, 114, 577
NmuCI GTSAC 3 cut(s) 397, 788, 2352
NsiI ATGCAT 1 cut(s) 1028
NspV TTCGAA 1 cut(s) 281
PciSI GCTCTTC 1 cut(s) 1236
PctI GAATGC 3 cut(s) 565, 625, 1445
PdmI GAANNNNTTC 3 cut(s) 752, 1258, 2086
PflMI CCANNNNNTGG 1 cut(s) 230
PkrI GCNGC 5 cut(s) 1434, 1533, 1746, 2020, 2023
PleI GAGTC 6 cut(s) 736, 1138, 1316, 1393, 1590, 2017
PmaCI CACGTG 1 cut(s) 1927
PmlI CACGTG 1 cut(s) 1927
PpsI GAGTC 6 cut(s) 736, 1138, 1316, 1393, 1590, 2017
Ppu21I YACGTR 1 cut(s) 1927
PpuMI RGGWCCY 1 cut(s) 1981
Psp1406I AACGTT 1 cut(s) 1413
Psp5II RGGWCCY 1 cut(s) 1981
PspCI CACGTG 1 cut(s) 1927
PspEI GGTNACC 1 cut(s) 627
PspN4I GGNNCC 3 cut(s) 75, 114, 577
PspPI GGNCC 1 cut(s) 1981
PspPPI RGGWCCY 1 cut(s) 1981
PsrI GAACNNNNNNTAC 2 cut(s) 1338, 1370
PstI CTGCAG 1 cut(s) 1741
PstNI CAGNNNCTG 3 cut(s) 653, 910, 2027
PsuI RGATCY 3 cut(s) 171, 340, 1162
RsaI GTAC 2 cut(s) 114, 2213
RsaNI GTAC 2 cut(s) 113, 2212
RseI CAYNNNNRTG 1 cut(s) 1652
SapI GCTCTTC 1 cut(s) 1236
SatI GCNGC 5 cut(s) 1433, 1532, 1745, 2019, 2022
Sau3AI GATC 9 cut(s) 33, 102, 171, 340, 454, 677, 1162, 1296, 1615
Sau96I GGNCC 1 cut(s) 1981
SchI GAGTC 6 cut(s) 737, 1139, 1316, 1394, 1591, 2018
SfaNI GCATC 7 cut(s) 679, 994, 1013, 1035, 1644, 1956, 2323
SfcI CTRYAG 2 cut(s) 1737, 2207
SfuI TTCGAA 1 cut(s) 281
SinI GGWCC 1 cut(s) 1981
SmiMI CAYNNNNRTG 1 cut(s) 1652
SmlI CTYRAG 7 cut(s) 146, 806, 932, 1103, 1577, 1865, 2540
SmoI CTYRAG 7 cut(s) 146, 806, 932, 1103, 1577, 1865, 2540
SsiI CCGC 2 cut(s) 1657, 1744
SspMI CTAG 7 cut(s) 296, 569, 699, 771, 1775, 2330, 2560
StyI CCWWGG 2 cut(s) 568, 1473
TaiI ACGT 3 cut(s) 946, 1416, 1929
TatI WGTACW 1 cut(s) 2211
TauI GCSGC 1 cut(s) 1747
TscAI CASTG 2 cut(s) 210, 2221
TseFI GTSAC 3 cut(s) 397, 788, 2352
TseI GCWGC 4 cut(s) 1432, 1531, 2018, 2021
Tsp45I GTSAC 3 cut(s) 397, 788, 2352
TspDTI ATGAA 9 cut(s) 168, 543, 729, 1083, 1587, 1650, 1970, 2085, 2274
TspGWI ACGGA 2 cut(s) 913, 2379
TspRI CASTG 2 cut(s) 210, 2221
Van91I CCANNNNNTGG 1 cut(s) 230
Vha464I CTTAAG 1 cut(s) 1103
VpaK11BI GGWCC 1 cut(s) 1981
XapI RAATTY 4 cut(s) 368, 434, 2072, 2176
XbaI TCTAGA 1 cut(s) 1774
XmaJI CCTAGG 1 cut(s) 568
XmiI GTMKAC 1 cut(s) 490
XmnI GAANNNNTTC 3 cut(s) 752, 1258, 2086
XspI CTAG 7 cut(s) 296, 569, 699, 771, 1775, 2330, 2560
Zsp2I ATGCAT 1 cut(s) 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.