MD07G1268600.v1.1

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007167
Physical Location & Seq
Forward (+)
33608137 .. 33610372
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1268600.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGTTCAAGTCATGGAGCAAGAAGAAGAAGATAAGAGCTGTTTTCCAACTGCAGTTTCAAGCAACGCAGGTGCCAAAATTGAAGAAGCCTGCTTTGATGCTGTCGCTGGTGCCGGACGACGTCGGAAAGGCAACGGTGAAGCTGGGGAAAGCGGCGGTTCAGGACGGCACCTGCATTTGGGAGAATCCAGTTTTTGAGTCAGTGAAGCTCATTGAGGATGTAAAAACAGGAAAACTGAAAGAGAAGATTTATCACTTCATTGTTTCAAATGGATCTTCGAAATCGGGGTATCTCGGAGAGGCTTCAATTGATTTTGCAGATATTGTGGCGGAAACTGAACCGTTAACCGTCGCACTGCCCCTTAAGTTTGCAAACTCTGGTGTGGTTTTACATGTGACAATGCACAGGATTCGGGAAGATGGTGATCAAAGGGAAATCGAAGAGAGTGACGATCCAAGTCTTTCGCGGCATAGCAGCGTAGATGGAAGCAACCACCAAAGCTTGAACGAAATGAATAAAGGGTATGTCCATCGGGACGCTGGCAGTTCCCTGTCGCCCCCTGAAGTACCAAATTCCATGCCTCAAAATGGACAGGCGGGTGCCAACGGAAGGAAAACCCATGTGCGTCAAAAATCAAGCTTGGATTGGTCTTCAGATGGAAGTTTATTTGACTCGCCCGACATTGTTGAAGACAAGCTTCCGACAGAAAGAGTGCAAGCGGTTTCAGAAGTTGAGAAACTCAGAAACGAAATTACTGTTCTGAAGAGGCAGGCAGGCCTATCAGAGCTGGAGTTGCAGTCTCTTCGGAAACAGATGGCCAAAGAGAGCAATCAAGGACAGAATCTGTCTAGACAGATTGTTTGCCTTAAAGAGGAGAGAGATGCGCTCAAAATGGAATGCGAACAACTTAAGTCCTCGCAGGGACGTGGTAATGGGAAACAGACTTTCAAAACGTTGCAGCCCGAGACTGAGGATACAAGAGCACAATTAGAAGCAATGAAGCAAGAGCTTAGTTCTGAAAAGAAAGCAAGAACCAATCTTCGCTCGCAACTTGCGCAGACACAAGACTCAAATTCTGAGTTAGTCCTTGTCGTGAAGGATCTCGAAGATGCATTGGGAAAGAAAAATAGACAGATATCTGATCTTTCAAGCAAGTTAGAAGCTGAAAAAAATTCCAAAGGGATGGGAACATATTCCGCAAGAAGAAAAAACGAGGATGATCAGGAAATAGAATCGTTGAAGCTTGACATCAGAGAACTTCTCAGCAAAATAGACACCCACCAGAAGAAGAGGGAAGAGCAGGATATGCGTATAAAACAGCTCAGCTTGGACTATGACATCGAAACCAAGAACGAATACGAACAGAGATGGAAAATGAGCGAGCGGGTTATATGGCTACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

468

Amino Acids

52.78

Weight (kDa)

7.11

Isoelectric Point (pI)

50.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 7 - 138 2.9e-19 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 58, 179
AatII GACGTC 1 cut(s) 123
Acc16I TGCGCA 1 cut(s) 1056
Acc36I ACCTGC 2 cut(s) 58, 179
AccB1I GGYRCC 4 cut(s) 70, 109, 167, 599
AccBSI CCGCTC 1 cut(s) 1384
AccII CGCG 1 cut(s) 466
AciI CCGC 8 cut(s) 152, 155, 329, 466, 596, 719, 1197, 1384
AclI AACGTT 1 cut(s) 953
AclWI GGATC 3 cut(s) 280, 446, 1107
AcoI YGGCCR 1 cut(s) 816
AcsI RAATTY 3 cut(s) 571, 1072, 1171
AcuI CTGAAG 3 cut(s) 582, 636, 782
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 4 cut(s) 177, 587, 609, 871
AflII CTTAAG 2 cut(s) 362, 908
AflIII ACRYGT 1 cut(s) 391
AjiI CACGTC 1 cut(s) 926
AjuI GAANNNNNNNTTGG 2 cut(s) 39, 71
Alw21I GWGCWC 1 cut(s) 985
Alw26I GTCTC 2 cut(s) 804, 959
AlwI GGATC 3 cut(s) 280, 446, 1107
AlwNI CAGNNNCTG 1 cut(s) 844
Ama87I CYCGRG 1 cut(s) 962
AoxI GGCC 2 cut(s) 775, 816
ApeKI GCWGC 2 cut(s) 474, 958
ApoI RAATTY 3 cut(s) 571, 1072, 1171
ArsI GACNNNNNNTTYG 2 cut(s) 843, 875
Asp700I GAANNNNTTC 1 cut(s) 1192
AspLEI GCGC 2 cut(s) 886, 1057
AsuHPI GGTGA 2 cut(s) 148, 434
AsuII TTCGAA 1 cut(s) 278
AvaI CYCGRG 1 cut(s) 962
BalI TGGCCA 1 cut(s) 818
BanI GGYRCC 4 cut(s) 70, 109, 167, 599
BbsI GAAGAC 2 cut(s) 642, 696
Bbv12I GWGCWC 1 cut(s) 985
BbvI GCAGC 2 cut(s) 486, 970
BccI CCATC 7 cut(s) 413, 476, 537, 650, 808, 1177, 1362
BceAI ACGGC 1 cut(s) 181
BcgI CGANNNNNNTGC 4 cut(s) 392, 426, 785, 819
BciVI GTATCC 1 cut(s) 967
BclI TGATCA 2 cut(s) 424, 1219
BcoDI GTCTC 2 cut(s) 804, 959
BfaI CTAG 1 cut(s) 849
BfmI CTRYAG 1 cut(s) 50
BfrI CTTAAG 2 cut(s) 362, 908
BfuAI ACCTGC 2 cut(s) 58, 179
BfuI GTATCC 1 cut(s) 967
BisI GCNGC 4 cut(s) 153, 467, 475, 959
BlpI GCTNAGC 1 cut(s) 1322
BlsI GCNGC 4 cut(s) 154, 468, 476, 960
BmeT110I CYCGRG 1 cut(s) 962
BmgBI CACGTC 1 cut(s) 926
BmiI GGNNCC 4 cut(s) 72, 111, 169, 601
BmsI GCATC 3 cut(s) 87, 871, 1099
BpiI GAAGAC 2 cut(s) 642, 696
BplI GAGNNNNNCTC 4 cut(s) 870, 902, 1245, 1277
BpmI CTGGAG 1 cut(s) 809
Bpu1102I GCTNAGC 1 cut(s) 1322
Bpu14I TTCGAA 1 cut(s) 278
BsaBI GATNNNNATC 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 587, 609, 871
Bse1I ACTGG 1 cut(s) 188
Bse3DI GCAATG 1 cut(s) 1002
Bse8I GATNNNNATC 1 cut(s) 423
BseGI GGATG 3 cut(s) 223, 1188, 1222
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 4 cut(s) 177, 587, 609, 871
BseMI GCAATG 1 cut(s) 1002
BseMII CTCAG 5 cut(s) 754, 960, 1068, 1276, 1336
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 887
BseXI GCAGC 2 cut(s) 486, 970
BseYI CCCAGC 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 2 cut(s) 777, 818
BshNI GGYRCC 4 cut(s) 70, 109, 167, 599
BsiHKAI GWGCWC 1 cut(s) 985
BsiHKCI CYCGRG 1 cut(s) 962
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 2 cut(s) 548, 936
BslI CCNNNNNNNGG 4 cut(s) 177, 587, 609, 871
BsmAI GTCTC 2 cut(s) 804, 959
BsmFI GGGAC 2 cut(s) 548, 936
BsmI GAATGC 1 cut(s) 902
BsnI GGCC 2 cut(s) 777, 818
BsoBI CYCGRG 1 cut(s) 962
Bsp119I TTCGAA 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 985
Bsp143I GATC 6 cut(s) 272, 424, 451, 1099, 1141, 1219
Bsp1720I GCTNAGC 1 cut(s) 1322
BspACI CCGC 8 cut(s) 152, 155, 329, 466, 596, 719, 1197, 1384
BspANI GGCC 2 cut(s) 777, 818
BspCNI CTCAG 5 cut(s) 753, 961, 1069, 1275, 1335
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 4 cut(s) 72, 111, 169, 601
BspMAI CTGCAG 1 cut(s) 54
BspMI ACCTGC 2 cut(s) 58, 179
BspPI GGATC 3 cut(s) 280, 446, 1107
BspQI GCTCTTC 1 cut(s) 1290
BspT104I TTCGAA 1 cut(s) 278
BspT107I GGYRCC 4 cut(s) 70, 109, 167, 599
BspTI CTTAAG 2 cut(s) 362, 908
BsrBI CCGCTC 1 cut(s) 1384
BsrDI GCAATG 1 cut(s) 1002
BsrI ACTGG 1 cut(s) 188
BssMI GATC 6 cut(s) 272, 424, 451, 1099, 1141, 1219
BssNI GRCGYC 1 cut(s) 120
Bst4CI ACNGT 4 cut(s) 136, 342, 349, 757
Bst6I CTCTTC 5 cut(s) 435, 758, 807, 1283, 1290
BstACI GRCGYC 1 cut(s) 120
BstAFI CTTAAG 2 cut(s) 362, 908
BstAPI GCANNNNNTGC 1 cut(s) 1306
BstBI TTCGAA 1 cut(s) 278
BstC8I GCNNGC 7 cut(s) 90, 541, 717, 771, 775, 1046, 1382
BstDEI CTNAG 6 cut(s) 740, 969, 1010, 1077, 1262, 1322
BstENI CCTNNNNNAGG 1 cut(s) 869
BstF5I GGATG 3 cut(s) 223, 1188, 1222
BstFNI CGCG 1 cut(s) 466
BstHHI GCGC 2 cut(s) 886, 1057
BstKTI GATC 6 cut(s) 275, 427, 454, 1102, 1144, 1222
BstMAI GTCTC 2 cut(s) 804, 959
BstMBI GATC 6 cut(s) 272, 424, 451, 1099, 1141, 1219
BstMWI GCNNNNNNNGC 3 cut(s) 793, 1054, 1306
BstNSI RCATGY 1 cut(s) 395
BstSFI CTRYAG 1 cut(s) 50
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 2 cut(s) 486, 970
BstV2I GAAGAC 2 cut(s) 642, 696
BstX2I RGATCY 2 cut(s) 272, 1099
BstXI CCANNNNNNTGG 1 cut(s) 1183
BstYI RGATCY 2 cut(s) 272, 1099
BsuI GTATCC 1 cut(s) 967
BsuRI GGCC 2 cut(s) 777, 818
BtrI CACGTC 1 cut(s) 926
BtsCI GGATG 3 cut(s) 223, 1188, 1222
BtsI GCAGTG 1 cut(s) 353
BtsIMutI CAGTG 2 cut(s) 207, 353
BveI ACCTGC 2 cut(s) 58, 179
Cac8I GCNNGC 7 cut(s) 90, 541, 717, 771, 775, 1046, 1382
CaiI CAGNNNCTG 1 cut(s) 844
CfoI GCGC 2 cut(s) 886, 1057
CseI GACGC 2 cut(s) 545, 614
Csp6I GTAC 1 cut(s) 566
CviAII CATG 4 cut(s) 12, 392, 577, 620
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 6 cut(s) 740, 969, 1010, 1077, 1262, 1322
DpnI GATC 6 cut(s) 274, 426, 453, 1101, 1143, 1221
DpnII GATC 6 cut(s) 272, 424, 451, 1099, 1141, 1219
EaeI YGGCCR 1 cut(s) 816
Eam1104I CTCTTC 5 cut(s) 435, 758, 807, 1283, 1290
EarI CTCTTC 5 cut(s) 435, 758, 807, 1283, 1290
EciI GGCGGA 1 cut(s) 344
Eco147I AGGCCT 1 cut(s) 777
Eco32I GATATC 1 cut(s) 1137
Eco57I CTGAAG 3 cut(s) 582, 636, 782
Eco88I CYCGRG 1 cut(s) 962
EcoNI CCTNNNNNAGG 1 cut(s) 869
EcoRV GATATC 1 cut(s) 1137
EcoT22I ATGCAT 1 cut(s) 1114
FaeI CATG 4 cut(s) 15, 395, 580, 623
FalI AAGNNNNNCTT 2 cut(s) 681, 713
FaqI GGGAC 2 cut(s) 548, 936
FatI CATG 4 cut(s) 11, 391, 576, 619
FauI CCCGC 2 cut(s) 589, 1377
FbaI TGATCA 2 cut(s) 424, 1219
Fnu4HI GCNGC 4 cut(s) 153, 467, 475, 959
FokI GGATG 3 cut(s) 230, 1195, 1229
Fsp4HI GCNGC 4 cut(s) 153, 467, 475, 959
FspBI CTAG 1 cut(s) 849
FspI TGCGCA 1 cut(s) 1056
GlaI GCGC 2 cut(s) 885, 1056
GluI GCNGC 4 cut(s) 153, 467, 475, 959
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 809
HaeIII GGCC 2 cut(s) 777, 818
HapII CCGG 1 cut(s) 113
HgaI GACGC 2 cut(s) 545, 614
HhaI GCGC 2 cut(s) 886, 1057
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 4 cut(s) 15, 395, 580, 623
Hin6I GCGC 2 cut(s) 884, 1055
HinP1I GCGC 2 cut(s) 884, 1055
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 4 cut(s) 499, 637, 695, 1241
HinfI GANTC 7 cut(s) 184, 197, 409, 671, 841, 1067, 1232
HpaI GTTAAC 1 cut(s) 345
HpaII CCGG 1 cut(s) 113
HphI GGTGA 2 cut(s) 148, 434
Hpy166II GTNNAC 1 cut(s) 345
Hpy188III TCNNGA 7 cut(s) 161, 413, 533, 849, 1093, 1103, 1223
Hpy8I GTNNAC 1 cut(s) 345
Hpy99I CGWCG 3 cut(s) 122, 125, 353
HpyAV CCTTC 2 cut(s) 603, 1090
HpyCH4III ACNGT 4 cut(s) 136, 342, 349, 757
HpyCH4IV ACGT 3 cut(s) 120, 925, 953
HpyCH4V TGCA 9 cut(s) 52, 174, 317, 371, 403, 715, 796, 958, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 793, 1054, 1306
HpyF3I CTNAG 6 cut(s) 740, 969, 1010, 1077, 1262, 1322
HpySE526I ACGT 3 cut(s) 120, 925, 953
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 4 cut(s) 15, 395, 580, 623
HspAI GCGC 2 cut(s) 884, 1055
Ksp22I TGATCA 2 cut(s) 424, 1219
KspAI GTTAAC 1 cut(s) 345
Kzo9I GATC 6 cut(s) 272, 424, 451, 1099, 1141, 1219
LguI GCTCTTC 1 cut(s) 1290
LmnI GCTCC 1 cut(s) 15
Lsp1109I GCAGC 2 cut(s) 486, 970
LweI GCATC 3 cut(s) 87, 871, 1099
MaeI CTAG 1 cut(s) 849
MaeII ACGT 3 cut(s) 120, 925, 953
MaeIII GTNAC 2 cut(s) 394, 446
MalI GATC 6 cut(s) 274, 426, 453, 1101, 1143, 1221
MbiI CCGCTC 1 cut(s) 1384
MboI GATC 6 cut(s) 272, 424, 451, 1099, 1141, 1219
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 2 cut(s) 272, 1099
MhlI GDGCHC 1 cut(s) 985
MlsI TGGCCA 1 cut(s) 818
MluCI AATT 7 cut(s) 77, 306, 571, 750, 986, 1072, 1171
MluNI TGGCCA 1 cut(s) 818
MlyI GAGTC 3 cut(s) 206, 665, 1061
MmeI TCCRAC 3 cut(s) 70, 103, 725
MnlI CCTC 9 cut(s) 208, 292, 591, 759, 865, 925, 964, 1207, 1284
Mox20I TGGCCA 1 cut(s) 818
Mph1103I ATGCAT 1 cut(s) 1114
MroXI GAANNNNTTC 1 cut(s) 1192
MscI TGGCCA 1 cut(s) 818
MseI TTAA 4 cut(s) 344, 363, 867, 909
Msp20I TGGCCA 1 cut(s) 818
MspCI CTTAAG 2 cut(s) 362, 908
MspI CCGG 1 cut(s) 113
MunI CAATTG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 902
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 3 cut(s) 793, 1054, 1306
NdeII GATC 6 cut(s) 272, 424, 451, 1099, 1141, 1219
NlaIII CATG 4 cut(s) 15, 395, 580, 623
NlaIV GGNNCC 4 cut(s) 72, 111, 169, 601
NmuCI GTSAC 2 cut(s) 394, 446
NsbI TGCGCA 1 cut(s) 1056
NsiI ATGCAT 1 cut(s) 1114
NspI RCATGY 1 cut(s) 395
NspV TTCGAA 1 cut(s) 278
PaqCI CACCTGC 2 cut(s) 58, 179
PceI AGGCCT 1 cut(s) 777
PciI ACATGT 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 1290
PctI GAATGC 1 cut(s) 902
PdmI GAANNNNTTC 1 cut(s) 1192
PfeI GAWTC 4 cut(s) 184, 409, 841, 1232
PflFI GACNNNGTC 1 cut(s) 119
PkrI GCNGC 4 cut(s) 154, 468, 476, 960
PleI GAGTC 3 cut(s) 205, 665, 1061
PpsI GAGTC 3 cut(s) 205, 665, 1061
PscI ACATGT 1 cut(s) 391
Psp1406I AACGTT 1 cut(s) 953
PspFI CCCAGC 1 cut(s) 142
PspN4I GGNNCC 4 cut(s) 72, 111, 169, 601
PstI CTGCAG 1 cut(s) 54
PstNI CAGNNNCTG 1 cut(s) 844
PsuI RGATCY 2 cut(s) 272, 1099
PsyI GACNNNGTC 1 cut(s) 119
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
SapI GCTCTTC 1 cut(s) 1290
SaqAI TTAA 4 cut(s) 344, 363, 867, 909
SatI GCNGC 4 cut(s) 153, 467, 475, 959
Sau3AI GATC 6 cut(s) 272, 424, 451, 1099, 1141, 1219
SchI GAGTC 3 cut(s) 206, 665, 1061
SduI GDGCHC 1 cut(s) 985
SfaNI GCATC 3 cut(s) 87, 871, 1099
SfcI CTRYAG 1 cut(s) 50
SfuI TTCGAA 1 cut(s) 278
SmlI CTYRAG 2 cut(s) 362, 908
SmoI CTYRAG 2 cut(s) 362, 908
Sse9I AATT 7 cut(s) 77, 306, 571, 750, 986, 1072, 1171
SseBI AGGCCT 1 cut(s) 777
SsiI CCGC 8 cut(s) 152, 155, 329, 466, 596, 719, 1197, 1384
SspMI CTAG 1 cut(s) 849
StuI AGGCCT 1 cut(s) 777
TaaI ACNGT 4 cut(s) 136, 342, 349, 757
TaiI ACGT 3 cut(s) 123, 928, 956
TaqI TCGA 4 cut(s) 278, 438, 1104, 1341
TasI AATT 7 cut(s) 77, 306, 571, 750, 986, 1072, 1171
TauI GCSGC 2 cut(s) 155, 469
TfiI GAWTC 4 cut(s) 184, 409, 841, 1232
Tru1I TTAA 4 cut(s) 344, 363, 867, 909
Tru9I TTAA 4 cut(s) 344, 363, 867, 909
TscAI CASTG 2 cut(s) 207, 360
TseFI GTSAC 2 cut(s) 394, 446
TseI GCWGC 2 cut(s) 474, 958
Tsp45I GTSAC 2 cut(s) 394, 446
TspDTI ATGAA 3 cut(s) 247, 527, 1013
TspGWI ACGGA 1 cut(s) 621
TspRI CASTG 2 cut(s) 207, 360
Tth111I GACNNNGTC 1 cut(s) 119
Vha464I CTTAAG 2 cut(s) 362, 908
XagI CCTNNNNNAGG 1 cut(s) 869
XapI RAATTY 3 cut(s) 571, 1072, 1171
XbaI TCTAGA 1 cut(s) 848
XceI RCATGY 1 cut(s) 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 1192
XspI CTAG 1 cut(s) 849
ZraI GACGTC 1 cut(s) 121
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.