FvH4_7g29461

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007167
Physical Location & Seq
Forward (+)
21665433 .. 21670011
4579 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29461.t1

Sequence Viewer

Length: 2685 bp
ATGTTCAGGACATGGAGCAAGAAGAAGAAGATAAAGGCTGTATTCCAATTGCAGTTTCAGGCAACAGAGGTGCCGAAATTGAAGAAGCCTGGGTTGATGATATCTCTGGTGCCCGACGACGTGGGGAAGCCGACGGTGAAACTGCCGAAGACTAAGGCTCAGGATGGGACCTGCACATGGGAGAATCCAGTTTATGAGTCAGTGAAGCTCGTGGAGGAGTCAAAATCAGGGAAACTGAAAGAAAAGATTTATCACTTCATTGTTTCCACTGGAACTTCGAAATCTGGTTATCTCGGAGAAGCTTCAATTGATTTTGCAGATTTTGTGGCAGAAACTGAACCCTTGACTGTCACTCTGCCACTTAAATTTGCAAATTCTGGAGTAGTTTTACATGTGACGATTCTCAGGGTGCAGGAAGATGGTGATTATAGAGAGATTGATGACAGTGAGGATCCTAGTCTTTCACGCCATAGCAGCGTGGACATCCAGAACAGCAATTGGGATACAGATGGAAGCAACCACCTAAGTTATACTGAAAATGGAGCTTTTGATAGAACAAGTAATGGTGCCAGTTCCGTGTCACCCCAAGTAAAAGACTCCATGCCTCAAAATGGAAAGGCCGGTACCACTGCAACAAAATTAGCCTTGGATTGGTCTTCAGATGAAAGCTCATTTGACTCACCAACTAGAGTTGAAGACAAGCCTCCAACGGAAAAAGTGAAAGATGTTTCAGGTGATTCCATTGAGAAACTCAGAAAGGAAAATGCAATGCTGATGAGGCAGGCGGATACATCAGAATTGGAATTACAGTCTCTCCGAAAGCAAGTCGCGAAAGAGAGCAGCCAAGGACAAAATCTGTCAAGACAGATAATTAGCCTTGAAGAGGAGAGAGATGCACTCAAATTAGAATGTCAACAATTAAAGTCATTGCGGGGACGTGGTGAGGAGGTACATGCTTCCCAAAAATTGCAGCCCGCGGACAAAGATACAAGAGTGCAGTTAGAAGCAATGAAGCAAGAGCTTAATCGTGAAAAGAAGGTAAAAGCCAATCTTCACTCACAACTGCAAACAACACAAGACTCGAATTCTGAGTTAGTCCTTGTGGTGAAGGATCTCGAAGAATTGTTGGTGAAGAAAAATAAGGAAATAGCTGATCTTTCAAACAAGTTAGAAACTGAAAAGAATAGCAAATTAATGGGGAAAATTTCCATACACAAAACAGATGTGGATGCCCAGGAAGTAGAGTCATTGAAGCAGAAGCTCCGAGAACTTTTGAGTGAAATGGACACCTACGAGAAGGAGAGGGAAGAACAAGATATGCATATAAAACGGCTCACCTTGGACTATGACCTTTTGAAGAAGAAAAACAATGACATCTCTCTGAAGTTAGATCGAAACCAAGAAAAGCTACGAACAGAGATGGAAAATGAGCGTGCAGGTTATATTGCGACCATAAAAGAACTTGAATCTCAAGTAAATAGATCAGAAGAAACAATTGAGAAGCAAGCCCATGAATTCGCAGAATGTTTGATCTCAATTCAGGAACTGGAAAATGAGGTCAAGTGTTTGGAGATGGAACTGGAGAAGCAGGCCAAGGGATTTGAAGATAAGCTGGAGGATCTGGCACATGCTAAAGATGAGCAAGAACAGAGAGCAAACAAAGCAGAAGAAGCGTTGAGAAAGACAAGGGGGGATAGTGCTGCTACAGCTGAGCGTCTACAAAATGAATTAAGAAAGCTTTCTATGGAAATGACATCTAAGGTTGATGAGAACGAGAAGCAGACCACTAAAGCACTGGCAGAAGCTAATGACCTGCGTGTGCAGAACAGAATTCTGGAAGAGATGCTCCAGAAATCCAATGAACAGTTAGAACTGATCAAGGGCCAGATTGATGCCAAAGCAAAGCATGTAGAGCAGATCTCCTCGGAACTAGAAAACAAAACGAAGCAGCTTGAATATGCATTACTGCACAAGGAAGAAAAGCATGAAGCTCTCACAATGAAAATCCAAATGCTCAAAGCTGAGATAGAAAGGCTCACAGAAGAGAACTCAAACATCACAAAACAGGAGGAAGAAAAATTGAGAGGAGATTTGGAACAAATGAAGAAACTAATTTCTGAAAATCAGGTGCTCATCCAGAATTTGAATGTTGAAAAGCATGATTTGGAGAAAAGATTGGCTTCATCAAAGCAGGAATCAGTGAAAACGCATGAAGAGCTTACTAACATAAAGTCCTTGAAAGATGAGAAGGAGACGGCAATCACTCATCTCAAGTCAGAAGTGGACAACCTGCGGACCCAGTACAATGAGTCTAAGAGCACCTTGCGTAAGGAAGCATTGGAAAAGGAAAACTTGAGTAAGCAAATACTTCAGTTACAGGCTGGGTTAAAGAAAGAAGGAAGAACCAGCACTGCAGAGAAGAAGCTCAAGAAATATAATGGACAAGCTGCTATTTCTAGTAATAACCAAAGGAAATCTGAAGACTCTGCGGAGAGAGAGCGTAATTATAAGGACTTGCTGACTGAGTTGACATTACTGAAAGAAAGAAACGCATCCATGGAAAAAGAACTGAAGGAGATGGAAGAGAGATATTCAGAGGTAAGTCTCAGATTTGCAGAGGTTGAAGGTGAAAGGCAACAACTTGTCATGACTGTGCGTAACCTCAGAAGCAATAAGAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

895

Amino Acids

102.4

Weight (kDa)

5.66

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 7 - 143 2.1e-20 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2508
Acc36I ACCTGC 4 cut(s) 179, 1427, 1821, 2298
Acc65I GGTACC 1 cut(s) 623
AccB1I GGYRCC 4 cut(s) 70, 109, 566, 623
AccI GTMKAC 1 cut(s) 1717
AccII CGCG 2 cut(s) 830, 977
AciI CCGC 6 cut(s) 785, 931, 975, 977, 2293, 2489
AclWI GGATC 4 cut(s) 446, 459, 1119, 1626
AcsI RAATTY 7 cut(s) 365, 373, 1084, 1203, 1514, 1830, 2140
AcuI CTGAAG 5 cut(s) 642, 1403, 2354, 2499, 2591
AfaI GTAC 3 cut(s) 625, 951, 2303
AfiI CCNNNNNNNGG 4 cut(s) 177, 611, 651, 883
AflIII ACRYGT 1 cut(s) 391
AjiI CACGTC 2 cut(s) 121, 938
AjnI CCWGG 2 cut(s) 88, 1233
AjuI GAANNNNNNNTTGG 2 cut(s) 39, 71
Alw21I GWGCWC 2 cut(s) 2133, 2321
Alw26I GTCTC 3 cut(s) 816, 2246, 2609
AlwI GGATC 4 cut(s) 446, 459, 1119, 1626
AlwNI CAGNNNCTG 2 cut(s) 335, 1546
AoxI GGCC 3 cut(s) 618, 1590, 1882
ApeKI GCWGC 6 cut(s) 474, 840, 970, 1700, 1948, 2447
ApoI RAATTY 7 cut(s) 365, 373, 1084, 1203, 1514, 1830, 2140
AseI ATTAAT 1 cut(s) 1193
Asp700I GAANNNNTTC 1 cut(s) 1738
Asp718I GGTACC 1 cut(s) 623
AspS9I GGNCC 3 cut(s) 168, 1882, 2295
AsuII TTCGAA 1 cut(s) 278
AvaII GGWCC 2 cut(s) 168, 2295
BaeGI GKGCMC 1 cut(s) 114
BaeI ACNNNNGTAYC 2 cut(s) 978, 1011
BamHI GGATCC 1 cut(s) 451
BanI GGYRCC 4 cut(s) 70, 109, 566, 623
BauI CACGAG 1 cut(s) 209
BbsI GAAGAC 4 cut(s) 155, 648, 702, 2487
Bbv12I GWGCWC 2 cut(s) 2133, 2321
BbvI GCAGC 6 cut(s) 486, 852, 982, 1687, 1960, 2434
BccI CCATC 6 cut(s) 158, 413, 503, 1414, 1567, 2572
BceAI ACGGC 2 cut(s) 1346, 2271
BciT130I CCWGG 2 cut(s) 90, 1235
BciVI GTATCC 2 cut(s) 496, 781
BclI TGATCA 1 cut(s) 1875
BcoDI GTCTC 3 cut(s) 816, 2246, 2609
BfaI CTAG 5 cut(s) 456, 687, 1931, 2457, 2683
BfmI CTRYAG 2 cut(s) 1704, 2412
BfuAI ACCTGC 4 cut(s) 179, 1427, 1821, 2298
BfuI GTATCC 2 cut(s) 496, 781
BglII AGATCT 1 cut(s) 1917
BisI GCNGC 6 cut(s) 475, 841, 971, 1701, 1949, 2448
BlpI GCTNAGC 1 cut(s) 1710
BlsI GCNGC 6 cut(s) 476, 842, 972, 1702, 1950, 2449
Bme1390I CCNGG 2 cut(s) 90, 1235
Bme18I GGWCC 2 cut(s) 168, 2295
BmgBI CACGTC 2 cut(s) 121, 938
BmgT120I GGNCC 3 cut(s) 168, 1882, 2295
BmiI GGNNCC 7 cut(s) 72, 111, 169, 453, 568, 625, 2297
BmrFI CCNGG 2 cut(s) 90, 1235
BmrI ACTGGG 1 cut(s) 2293
BmsI GCATC 5 cut(s) 883, 1219, 1833, 1882, 2561
BmuI ACTGGG 1 cut(s) 2293
BpiI GAAGAC 4 cut(s) 155, 648, 702, 2487
BplI GAGNNNNNCTC 4 cut(s) 882, 914, 1904, 1936
BpmI CTGGAG 4 cut(s) 399, 1601, 1634, 1832
Bpu10I CCTNAGC 1 cut(s) 159
Bpu1102I GCTNAGC 1 cut(s) 1710
Bpu14I TTCGAA 1 cut(s) 278
BpuEI CTTGAG 4 cut(s) 1455, 2255, 2374, 2411
BsaJI CCNNGG 9 cut(s) 89, 645, 844, 975, 1233, 1338, 1593, 1923, 2556
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 611, 651, 883
Bse118I RCCGGY 1 cut(s) 620
Bse1I ACTGG 7 cut(s) 188, 274, 570, 1551, 1584, 1800, 2299
Bse3DI GCAATG 3 cut(s) 774, 926, 1014
BseBI CCWGG 2 cut(s) 90, 1235
BseDI CCNNGG 9 cut(s) 89, 645, 844, 975, 1233, 1338, 1593, 1923, 2556
BseGI GGATG 5 cut(s) 169, 483, 1234, 2133, 2552
BseLI CCNNNNNNNGG 4 cut(s) 177, 611, 651, 883
BseMI GCAATG 3 cut(s) 774, 926, 1014
BseMII CTCAG 9 cut(s) 173, 418, 766, 1080, 1701, 2013, 2514, 2620, 2677
BseNI ACTGG 7 cut(s) 188, 274, 570, 1551, 1584, 1800, 2299
BseRI GAGGAG 5 cut(s) 230, 899, 959, 1912, 2100
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 6 cut(s) 486, 852, 982, 1687, 1960, 2434
BseYI CCCAGC 1 cut(s) 2381
BsgI GTGCAG 6 cut(s) 157, 431, 1016, 1455, 1841, 1952
Bsh1236I CGCG 2 cut(s) 830, 977
BshFI GGCC 3 cut(s) 620, 1592, 1884
BshNI GGYRCC 4 cut(s) 70, 109, 566, 623
BsiHKAI GWGCWC 2 cut(s) 2133, 2321
BsiSI CCGG 1 cut(s) 621
BslFI GGGAC 2 cut(s) 181, 948
BslI CCNNNNNNNGG 4 cut(s) 177, 611, 651, 883
BsmAI GTCTC 3 cut(s) 816, 2246, 2609
BsmBI CGTCTC 1 cut(s) 2246
BsmFI GGGAC 2 cut(s) 181, 948
BsnI GGCC 3 cut(s) 620, 1592, 1884
Bsp119I TTCGAA 1 cut(s) 278
Bsp1286I GDGCHC 3 cut(s) 114, 2133, 2321
Bsp143I GATC 9 cut(s) 451, 1111, 1153, 1390, 1481, 1530, 1618, 1875, 1917
Bsp1720I GCTNAGC 1 cut(s) 1710
Bsp19I CCATGG 1 cut(s) 2556
Bsp68I TCGCGA 1 cut(s) 830
BspACI CCGC 6 cut(s) 785, 931, 975, 977, 2293, 2489
BspANI GGCC 3 cut(s) 620, 1592, 1884
BspCNI CTCAG 9 cut(s) 172, 417, 765, 1081, 1702, 2014, 2515, 2619, 2676
BspFNI CGCG 2 cut(s) 830, 977
BspHI TCATGA 1 cut(s) 2646
BspLI GGNNCC 7 cut(s) 72, 111, 169, 453, 568, 625, 2297
BspMAI CTGCAG 1 cut(s) 2416
BspMI ACCTGC 4 cut(s) 179, 1427, 1821, 2298
BspPI GGATC 4 cut(s) 446, 459, 1119, 1626
BspQI GCTCTTC 1 cut(s) 2208
BspT104I TTCGAA 1 cut(s) 278
BspT107I GGYRCC 4 cut(s) 70, 109, 566, 623
BsrDI GCAATG 3 cut(s) 774, 926, 1014
BsrFI RCCGGY 1 cut(s) 620
BsrI ACTGG 7 cut(s) 188, 274, 570, 1551, 1584, 1800, 2299
BssAI RCCGGY 1 cut(s) 620
BssECI CCNNGG 9 cut(s) 89, 645, 844, 975, 1233, 1338, 1593, 1923, 2556
BssMI GATC 9 cut(s) 451, 1111, 1153, 1390, 1481, 1530, 1618, 1875, 1917
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 5 cut(s) 645, 844, 1338, 1593, 2556
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 90, 1235
Bst4CI ACNGT 6 cut(s) 136, 349, 446, 810, 1866, 2653
Bst6I CTCTTC 5 cut(s) 876, 1833, 2037, 2208, 2577
BstBI TTCGAA 1 cut(s) 278
BstC8I GCNNGC 5 cut(s) 783, 975, 1434, 1506, 1590
BstDSI CCRYGG 2 cut(s) 975, 2556
BstENI CCTNNNNNAGG 1 cut(s) 881
BstF5I GGATG 5 cut(s) 169, 483, 1234, 2133, 2552
BstFNI CGCG 2 cut(s) 830, 977
BstKTI GATC 9 cut(s) 454, 1114, 1156, 1393, 1484, 1533, 1621, 1878, 1920
BstMAI GTCTC 3 cut(s) 816, 2246, 2609
BstMBI GATC 9 cut(s) 451, 1111, 1153, 1390, 1481, 1530, 1618, 1875, 1917
BstMWI GCNNNNNNNGC 7 cut(s) 474, 778, 1661, 1670, 1706, 1912, 2215
BstNI CCWGG 2 cut(s) 90, 1235
BstNSI RCATGY 4 cut(s) 395, 956, 1631, 1910
BstSCI CCNGG 2 cut(s) 88, 1233
BstSFI CTRYAG 2 cut(s) 1704, 2412
BstSLI GKGCMC 1 cut(s) 114
BstUI CGCG 2 cut(s) 830, 977
BstV1I GCAGC 6 cut(s) 486, 852, 982, 1687, 1960, 2434
BstV2I GAAGAC 4 cut(s) 155, 648, 702, 2487
BstX2I RGATCY 4 cut(s) 451, 1111, 1618, 1917
BstYI RGATCY 4 cut(s) 451, 1111, 1618, 1917
BsuI GTATCC 2 cut(s) 496, 781
BsuRI GGCC 3 cut(s) 620, 1592, 1884
BtgI CCRYGG 2 cut(s) 975, 2556
BtrI CACGTC 2 cut(s) 121, 938
BtsCI GGATG 5 cut(s) 169, 483, 1234, 2133, 2552
BtsI GCAGTG 2 cut(s) 627, 2409
BtsIMutI CAGTG 7 cut(s) 207, 267, 451, 627, 1793, 2205, 2409
BtuMI TCGCGA 1 cut(s) 830
BveI ACCTGC 4 cut(s) 179, 1427, 1821, 2298
Cac8I GCNNGC 5 cut(s) 783, 975, 1434, 1506, 1590
CaiI CAGNNNCTG 2 cut(s) 335, 1546
CciI TCATGA 1 cut(s) 2646
Cfr10I RCCGGY 1 cut(s) 620
Cfr13I GGNCC 3 cut(s) 168, 1882, 2295
Cfr42I CCGCGG 1 cut(s) 978
CseI GACGC 1 cut(s) 1703
Csp6I GTAC 3 cut(s) 624, 950, 2302
CviQI GTAC 3 cut(s) 624, 950, 2302
DpnI GATC 9 cut(s) 453, 1113, 1155, 1392, 1483, 1532, 1620, 1877, 1919
DpnII GATC 9 cut(s) 451, 1111, 1153, 1390, 1481, 1530, 1618, 1875, 1917
Eam1104I CTCTTC 5 cut(s) 876, 1833, 2037, 2208, 2577
EarI CTCTTC 5 cut(s) 876, 1833, 2037, 2208, 2577
EciI GGCGGA 1 cut(s) 800
Eco130I CCWWGG 5 cut(s) 645, 844, 1338, 1593, 2556
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 2 cut(s) 168, 2295
Eco57I CTGAAG 5 cut(s) 642, 1403, 2354, 2499, 2591
EcoNI CCTNNNNNAGG 1 cut(s) 881
EcoO109I RGGNCCY 1 cut(s) 168
EcoRI GAATTC 3 cut(s) 1084, 1514, 1830
EcoRII CCWGG 2 cut(s) 88, 1233
EcoRV GATATC 1 cut(s) 102
EcoT14I CCWWGG 5 cut(s) 645, 844, 1338, 1593, 2556
EcoT22I ATGCAT 2 cut(s) 1323, 1963
ErhI CCWWGG 5 cut(s) 645, 844, 1338, 1593, 2556
Esp3I CGTCTC 1 cut(s) 2246
FalI AAGNNNNNCTT 8 cut(s) 1035, 1067, 2164, 2196, 2306, 2338, 2336, 2368
FaqI GGGAC 2 cut(s) 181, 948
FauI CCCGC 2 cut(s) 924, 982
FbaI TGATCA 1 cut(s) 1875
FblI GTMKAC 1 cut(s) 1717
Fnu4HI GCNGC 6 cut(s) 475, 841, 971, 1701, 1949, 2448
FokI GGATG 5 cut(s) 176, 470, 1241, 2120, 2539
Fsp4HI GCNGC 6 cut(s) 475, 841, 971, 1701, 1949, 2448
FspBI CTAG 5 cut(s) 456, 687, 1931, 2457, 2683
GluI GCNGC 6 cut(s) 475, 841, 971, 1701, 1949, 2448
GsaI CCCAGC 1 cut(s) 2385
GsuI CTGGAG 4 cut(s) 399, 1601, 1634, 1832
HaeIII GGCC 3 cut(s) 620, 1592, 1884
HapII CCGG 1 cut(s) 621
HgaI GACGC 1 cut(s) 1703
HincII GTYRAC 2 cut(s) 914, 2529
HindII GTYRAC 2 cut(s) 914, 2529
HindIII AAGCTT 2 cut(s) 300, 1736
HpaII CCGG 1 cut(s) 621
Hpy166II GTNNAC 5 cut(s) 481, 914, 1718, 2284, 2529
Hpy8I GTNNAC 5 cut(s) 481, 914, 1718, 2284, 2529
Hpy99I CGWCG 3 cut(s) 119, 122, 136
HpyAV CCTTC 7 cut(s) 1030, 1102, 1291, 2242, 2390, 2566, 2618
HpyCH4III ACNGT 6 cut(s) 136, 349, 446, 810, 1866, 2653
HpyCH4IV ACGT 2 cut(s) 120, 937
HpyF10VI GCNNNNNNNGC 7 cut(s) 474, 778, 1661, 1670, 1706, 1912, 2215
HpySE526I ACGT 2 cut(s) 120, 937
KpnI GGTACC 1 cut(s) 627
Ksp22I TGATCA 1 cut(s) 1875
KspI CCGCGG 1 cut(s) 978
Kzo9I GATC 9 cut(s) 451, 1111, 1153, 1390, 1481, 1530, 1618, 1875, 1917
LguI GCTCTTC 1 cut(s) 2208
LmnI GCTCC 4 cut(s) 15, 542, 1266, 1851
Lsp1109I GCAGC 6 cut(s) 486, 852, 982, 1687, 1960, 2434
LweI GCATC 5 cut(s) 883, 1219, 1833, 1882, 2561
MaeI CTAG 5 cut(s) 456, 687, 1931, 2457, 2683
MaeII ACGT 2 cut(s) 120, 937
MaeIII GTNAC 5 cut(s) 349, 394, 579, 2373, 2657
MalI GATC 9 cut(s) 453, 1113, 1155, 1392, 1483, 1532, 1620, 1877, 1919
MboI GATC 9 cut(s) 451, 1111, 1153, 1390, 1481, 1530, 1618, 1875, 1917
MfeI CAATTG 4 cut(s) 47, 306, 496, 1494
MflI RGATCY 4 cut(s) 451, 1111, 1618, 1917
MhlI GDGCHC 3 cut(s) 114, 2133, 2321
MlyI GAGTC 8 cut(s) 206, 227, 590, 671, 1073, 1253, 2318, 2477
MmeI TCCRAC 1 cut(s) 731
Mph1103I ATGCAT 2 cut(s) 1323, 1963
MroXI GAANNNNTTC 1 cut(s) 1738
MseI TTAA 6 cut(s) 363, 920, 1023, 1193, 1730, 2387
MslI CAYNNNNRTG 1 cut(s) 2651
MspA1I CMGCKG 2 cut(s) 977, 1709
MspI CCGG 1 cut(s) 621
MspR9I CCNGG 2 cut(s) 90, 1235
MunI CAATTG 4 cut(s) 47, 306, 496, 1494
MvaI CCWGG 2 cut(s) 90, 1235
MvnI CGCG 2 cut(s) 830, 977
MwoI GCNNNNNNNGC 7 cut(s) 474, 778, 1661, 1670, 1706, 1912, 2215
NcoI CCATGG 1 cut(s) 2556
NdeII GATC 9 cut(s) 451, 1111, 1153, 1390, 1481, 1530, 1618, 1875, 1917
NlaIV GGNNCC 7 cut(s) 72, 111, 169, 453, 568, 625, 2297
NmuCI GTSAC 3 cut(s) 349, 394, 579
NruI TCGCGA 1 cut(s) 830
NsiI ATGCAT 2 cut(s) 1323, 1963
NspI RCATGY 4 cut(s) 395, 956, 1631, 1910
NspV TTCGAA 1 cut(s) 278
PagI TCATGA 1 cut(s) 2646
PciI ACATGT 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 2208
PdmI GAANNNNTTC 1 cut(s) 1738
PfeI GAWTC 5 cut(s) 184, 400, 737, 1466, 2195
PkrI GCNGC 6 cut(s) 476, 842, 972, 1702, 1950, 2449
PleI GAGTC 8 cut(s) 205, 226, 590, 671, 1073, 1252, 2317, 2477
PpsI GAGTC 8 cut(s) 205, 226, 590, 671, 1073, 1252, 2317, 2477
PpuMI RGGWCCY 1 cut(s) 168
PscI ACATGT 1 cut(s) 391
PshBI ATTAAT 1 cut(s) 1193
PsiI TTATAA 1 cut(s) 2508
Psp5II RGGWCCY 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 88, 1233
PspFI CCCAGC 1 cut(s) 2381
PspGI CCWGG 2 cut(s) 88, 1233
PspN4I GGNNCC 7 cut(s) 72, 111, 169, 453, 568, 625, 2297
PspPI GGNCC 3 cut(s) 168, 1882, 2295
PspPPI RGGWCCY 1 cut(s) 168
PstI CTGCAG 1 cut(s) 2416
PstNI CAGNNNCTG 2 cut(s) 335, 1546
PsuI RGATCY 4 cut(s) 451, 1111, 1618, 1917
PvuII CAGCTG 1 cut(s) 1709
RruI TCGCGA 1 cut(s) 830
RsaI GTAC 3 cut(s) 625, 951, 2303
RsaNI GTAC 3 cut(s) 624, 950, 2302
RseI CAYNNNNRTG 1 cut(s) 2651
SacII CCGCGG 1 cut(s) 978
SapI GCTCTTC 1 cut(s) 2208
SaqAI TTAA 6 cut(s) 363, 920, 1023, 1193, 1730, 2387
SatI GCNGC 6 cut(s) 475, 841, 971, 1701, 1949, 2448
Sau3AI GATC 9 cut(s) 451, 1111, 1153, 1390, 1481, 1530, 1618, 1875, 1917
Sau96I GGNCC 3 cut(s) 168, 1882, 2295
SchI GAGTC 8 cut(s) 206, 227, 590, 671, 1073, 1253, 2318, 2477
ScrFI CCNGG 2 cut(s) 90, 1235
SduI GDGCHC 3 cut(s) 114, 2133, 2321
SfaNI GCATC 5 cut(s) 883, 1219, 1833, 1882, 2561
SfcI CTRYAG 2 cut(s) 1704, 2412
Sfr303I CCGCGG 1 cut(s) 978
SfuI TTCGAA 1 cut(s) 278
SgrBI CCGCGG 1 cut(s) 978
SinI GGWCC 2 cut(s) 168, 2295
SmiMI CAYNNNNRTG 1 cut(s) 2651
SmlI CTYRAG 4 cut(s) 1470, 2270, 2353, 2426
SmoI CTYRAG 4 cut(s) 1470, 2270, 2353, 2426
SsiI CCGC 6 cut(s) 785, 931, 975, 977, 2293, 2489
SspMI CTAG 5 cut(s) 456, 687, 1931, 2457, 2683
StyD4I CCNGG 2 cut(s) 88, 1233
StyI CCWWGG 5 cut(s) 645, 844, 1338, 1593, 2556
TaaI ACNGT 6 cut(s) 136, 349, 446, 810, 1866, 2653
TaiI ACGT 2 cut(s) 123, 940
TaqI TCGA 4 cut(s) 278, 1082, 1116, 1393
TatI WGTACW 1 cut(s) 2301
TfiI GAWTC 5 cut(s) 184, 400, 737, 1466, 2195
Tru1I TTAA 6 cut(s) 363, 920, 1023, 1193, 1730, 2387
Tru9I TTAA 6 cut(s) 363, 920, 1023, 1193, 1730, 2387
TscAI CASTG 7 cut(s) 207, 274, 451, 634, 1800, 2205, 2416
TseFI GTSAC 3 cut(s) 349, 394, 579
TseI GCWGC 6 cut(s) 474, 840, 970, 1700, 1948, 2447
Tsp45I GTSAC 3 cut(s) 349, 394, 579
TspGWI ACGGA 2 cut(s) 565, 725
TspRI CASTG 7 cut(s) 207, 274, 451, 634, 1800, 2205, 2416
VpaK11BI GGWCC 2 cut(s) 168, 2295
VspI ATTAAT 1 cut(s) 1193
XagI CCTNNNNNAGG 1 cut(s) 881
XapI RAATTY 7 cut(s) 365, 373, 1084, 1203, 1514, 1830, 2140
XceI RCATGY 4 cut(s) 395, 956, 1631, 1910
XcmI CCANNNNNNNNNTGG 1 cut(s) 1792
XmiI GTMKAC 1 cut(s) 1717
XmnI GAANNNNTTC 1 cut(s) 1738
XspI CTAG 5 cut(s) 456, 687, 1931, 2457, 2683
Zsp2I ATGCAT 2 cut(s) 1323, 1963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.