AT5G52500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012276
Physical Location & Seq
Forward (+)
21301525 .. 21302829
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52500.1

Sequence Viewer

Length: 1092 bp
ATGGGTTTGTTCTTAGTCCTATTCCCCGACCACAACAACAACGACTCTTCTTCTTATTATTCTCCGGCAAAAACTACCTTCCGCTCCAAATTATCCTCTCGTCTTCTCCTCTCCAAGGCTGAATCCACAATCTCAAGCTGCATCATCCTCCTCTTCCTCACTCTCTTCCTCTTCACTCTCTCTACATTCGAAGAACCTTCCTCTCGTTTTCCCGCCATTTCTTCTCCTCACTGGCGATTTCTCCTCAACCGCAACATCATCTCTCGTCGTTTCGCTCTACAAGGAATGGGTACTCTGTTTCTAAGAGGAACCAAGAGCATGCACGATTTAATCATTGCTCATATCGCTTCCGTTACAACGGAGAACGATCTCCGTCTCTTTATACGGTTGCTTCACCGCTCCGGAGTTACTTCAAAATCCGACGTCGTTTTACTCTTCAATTCACCTTCATCCACTACAAGATTCACCGAGTTGATCGAACAAGAGAACAACTCGTTCTTAAAGCTCGTTCATGTTCATCGGAATGATTCGATGACATCATCATCATCATCATCTGAGCATGTTATTTGGGGGAAGAAGAGTCGTCATCGAGCTAATTATAACTCGAACGAGCTGACTCATGGCTCGATAGTTGGTTTCGACGTAACTGAGTTGGATCCTGAAAACTCTTTATCCGGGTTTATGGATCAAGTTCCGTTAAGCTTGAGGAGATGGGCTTGTTACCCAATGCTTCTTGGTCGAGTACGACGCAGTTTCAAACACGTTATGCTTGTTGACGCTAAAACTTCATTTTTCATCGGTGACCCGTTTACCCGGATTCGTAACCGGAGCCCCGACTCAGTCCTCTTCTTCTTCTCAAAACACAAGAACGCCTCCGAGGTTAATCCGGAGATTTTGATCGGTGGAGCTAAAGGAATCAGGAGATTGTCGAGCTCCATGCATACTGAGATTGTGAGAGCGACGATGATGAAGAAGAACTCGGTTACGGAAGTAACGGAATCGGTTGTGTTGAGTCAACTCGTCGGGAATGTTCATATGACGAAGAACTTTCAAGTGGTTTTAAGTGAGTCAGTGGTGCCGGAAGCGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

363

Amino Acids

40.99

Weight (kDa)

9.91

Isoelectric Point (pI)

63.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7780 PF25002 91 - 356 7.9e-105 Domain of unknown function (DUF7780)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57400 AT5G52500
fragaria_vesca FvH4_6g23940
malus_domestica MD12G1074600.v1.1 MD14G1072700.v1.1
prunus_persica Prupe.7G058900_v2.0.a1
pyrus_communis pycom12g06420 pycom14g06260
rosa_chinensis RchiOBHm_Chr3g0482601
rosa_laevigata RLG00000023338
rosa_multiflora Rmu_sc0000224.1_g000003 Rmu_sc0004687.1_g000003
rosa_roxburghii Rroxscaffold_6G00399090
rosa_rugosa Rorug03G0197500
rosa_samantha Rh3AG248600 Rh3BG284100 Rh3CG281600 Rh3DG277800
rosa_wichuraiana Rw3G022450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
AatII GACGTC 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 1075
AccBSI CCGCTC 2 cut(s) 84, 399
AccIII TCCGGA 2 cut(s) 401, 886
AciI CCGC 4 cut(s) 82, 213, 250, 397
AclWI GGATC 3 cut(s) 650, 663, 693
AcyI GRCGYC 1 cut(s) 423
AfaI GTAC 2 cut(s) 292, 744
AfiI CCNNNNNNNGG 1 cut(s) 115
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 4 cut(s) 414, 439, 757, 1052
AluBI AGCT 7 cut(s) 138, 505, 593, 613, 702, 908, 933
AluI AGCT 7 cut(s) 138, 505, 593, 613, 702, 908, 933
Alw21I GWGCWC 1 cut(s) 935
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 3 cut(s) 650, 663, 693
Aor13HI TCCGGA 2 cut(s) 401, 886
ApeKI GCWGC 1 cut(s) 138
AsuC2I CCSGG 2 cut(s) 676, 814
AsuHPI GGTGA 4 cut(s) 386, 435, 457, 812
AsuII TTCGAA 1 cut(s) 189
BamHI GGATCC 1 cut(s) 655
BanI GGYRCC 1 cut(s) 1075
BanII GRGCYC 2 cut(s) 833, 935
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 935
BbvI GCAGC 1 cut(s) 125
BccI CCATC 1 cut(s) 705
BcgI CGANNNNNNTGC 2 cut(s) 919, 953
BcnI CCSGG 2 cut(s) 676, 814
BcoDI GTCTC 1 cut(s) 380
BisI GCNGC 1 cut(s) 139
BlsI GCNGC 1 cut(s) 140
Bme1390I CCNGG 2 cut(s) 676, 814
BmiI GGNNCC 4 cut(s) 310, 657, 830, 1077
BmrFI CCNGG 2 cut(s) 676, 814
BmsI GCATC 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 95
BplI GAGNNNNNCTC 2 cut(s) 476, 508
Bpu14I TTCGAA 1 cut(s) 189
BpuEI CTTGAG 2 cut(s) 118, 724
BpuMI CCSGG 2 cut(s) 676, 814
BsaBI GATNNNNATC 1 cut(s) 896
BsaHI GRCGYC 1 cut(s) 423
BsaJI CCNNGG 2 cut(s) 114, 876
BsaWI WCCGGW 3 cut(s) 401, 825, 886
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 333
Bse8I GATNNNNATC 1 cut(s) 896
BseAI TCCGGA 2 cut(s) 401, 886
BseDI CCNNGG 2 cut(s) 114, 876
BseGI GGATG 2 cut(s) 144, 449
BseJI GATNNNNATC 1 cut(s) 896
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 4 cut(s) 546, 639, 852, 936
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 5 cut(s) 98, 140, 216, 233, 721
BseXI GCAGC 1 cut(s) 125
BshNI GGYRCC 1 cut(s) 1075
BsiHKAI GWGCWC 1 cut(s) 935
BsiSI CCGG 7 cut(s) 65, 402, 675, 814, 826, 887, 1079
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 380
BsmBI CGTCTC 1 cut(s) 380
Bsp119I TTCGAA 1 cut(s) 189
Bsp1286I GDGCHC 2 cut(s) 833, 935
Bsp13I TCCGGA 2 cut(s) 401, 886
Bsp143I GATC 5 cut(s) 367, 474, 655, 685, 897
BspACI CCGC 4 cut(s) 82, 213, 250, 397
BspCNI CTCAG 4 cut(s) 547, 640, 851, 937
BspEI TCCGGA 2 cut(s) 401, 886
BspLI GGNNCC 4 cut(s) 310, 657, 830, 1077
BspPI GGATC 3 cut(s) 650, 663, 693
BspT104I TTCGAA 1 cut(s) 189
BspT107I GGYRCC 1 cut(s) 1075
BsrBI CCGCTC 2 cut(s) 84, 399
BsrDI GCAATG 1 cut(s) 333
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 114, 876
BssMI GATC 5 cut(s) 367, 474, 655, 685, 897
BssNI GRCGYC 1 cut(s) 423
BssT1I CCWWGG 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 387
Bst6I CTCTTC 7 cut(s) 52, 158, 170, 176, 440, 572, 851
BstACI GRCGYC 1 cut(s) 423
BstBI TTCGAA 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 320
BstDEI CTNAG 6 cut(s) 13, 302, 555, 648, 838, 945
BstEII GGTNACC 1 cut(s) 800
BstENI CCTNNNNNAGG 1 cut(s) 113
BstF5I GGATG 2 cut(s) 144, 449
BstKTI GATC 5 cut(s) 370, 477, 658, 688, 900
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 5 cut(s) 367, 474, 655, 685, 897
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNSI RCATGY 2 cut(s) 322, 563
BstPI GGTNACC 1 cut(s) 800
BstSCI CCNGG 2 cut(s) 674, 812
BstV1I GCAGC 1 cut(s) 125
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 655
BstYI RGATCY 1 cut(s) 655
BtsCI GGATG 2 cut(s) 144, 449
BtsIMutI CAGTG 2 cut(s) 229, 1077
Cac8I GCNNGC 1 cut(s) 320
CseI GACGC 2 cut(s) 756, 785
Csp6I GTAC 2 cut(s) 291, 743
CviAII CATG 5 cut(s) 319, 512, 560, 620, 937
CviQI GTAC 2 cut(s) 291, 743
DdeI CTNAG 6 cut(s) 13, 302, 555, 648, 838, 945
DpnI GATC 5 cut(s) 369, 476, 657, 687, 899
DpnII GATC 5 cut(s) 367, 474, 655, 685, 897
Eam1104I CTCTTC 7 cut(s) 52, 158, 170, 176, 440, 572, 851
EarI CTCTTC 7 cut(s) 52, 158, 170, 176, 440, 572, 851
Ecl136II GAGCTC 1 cut(s) 933
Eco130I CCWWGG 1 cut(s) 114
Eco24I GRGCYC 2 cut(s) 833, 935
Eco53kI GAGCTC 1 cut(s) 933
Eco91I GGTNACC 1 cut(s) 800
EcoICRI GAGCTC 1 cut(s) 933
EcoNI CCTNNNNNAGG 1 cut(s) 113
EcoO65I GGTNACC 1 cut(s) 800
EcoT14I CCWWGG 1 cut(s) 114
EcoT22I ATGCAT 1 cut(s) 942
EcoT38I GRGCYC 2 cut(s) 833, 935
ErhI CCWWGG 1 cut(s) 114
Esp3I CGTCTC 1 cut(s) 380
FaeI CATG 5 cut(s) 322, 515, 563, 623, 940
FatI CATG 5 cut(s) 318, 511, 559, 619, 936
FauI CCCGC 1 cut(s) 220
FauNDI CATATG 1 cut(s) 1035
Fnu4HI GCNGC 1 cut(s) 139
FokI GGATG 2 cut(s) 131, 436
FriOI GRGCYC 2 cut(s) 833, 935
Fsp4HI GCNGC 1 cut(s) 139
GluI GCNGC 1 cut(s) 139
HapII CCGG 7 cut(s) 65, 402, 675, 814, 826, 887, 1079
HgaI GACGC 2 cut(s) 756, 785
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 5 cut(s) 322, 515, 563, 623, 940
HincII GTYRAC 2 cut(s) 775, 1016
HindII GTYRAC 2 cut(s) 775, 1016
HindIII AAGCTT 1 cut(s) 700
HpaII CCGG 7 cut(s) 65, 402, 675, 814, 826, 887, 1079
HphI GGTGA 4 cut(s) 386, 435, 457, 812
Hpy166II GTNNAC 3 cut(s) 775, 810, 1016
Hpy188I TCNGA 4 cut(s) 421, 522, 556, 877
Hpy188III TCNNGA 5 cut(s) 402, 659, 887, 919, 1024
Hpy8I GTNNAC 3 cut(s) 775, 810, 1016
Hpy99I CGWCG 7 cut(s) 270, 425, 428, 644, 750, 964, 1025
HpyAV CCTTC 3 cut(s) 88, 207, 456
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 3 cut(s) 423, 642, 762
HpyCH4V TGCA 3 cut(s) 141, 322, 940
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 6 cut(s) 13, 302, 555, 648, 838, 945
HpySE526I ACGT 3 cut(s) 423, 642, 762
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 5 cut(s) 322, 515, 563, 623, 940
Kpn2I TCCGGA 2 cut(s) 401, 886
Kzo9I GATC 5 cut(s) 367, 474, 655, 685, 897
LmnI GCTCC 5 cut(s) 89, 404, 828, 905, 938
LpnPI CCDG 9 cut(s) 78, 217, 415, 672, 688, 827, 839, 900, 904
Lsp1109I GCAGC 1 cut(s) 125
LweI GCATC 1 cut(s) 150
MaeII ACGT 3 cut(s) 423, 642, 762
MaeIII GTNAC 8 cut(s) 352, 406, 643, 719, 800, 821, 982, 991
MalI GATC 5 cut(s) 369, 476, 657, 687, 899
MbiI CCGCTC 2 cut(s) 84, 399
MboI GATC 5 cut(s) 367, 474, 655, 685, 897
MflI RGATCY 1 cut(s) 655
MhlI GDGCHC 2 cut(s) 833, 935
MluCI AATT 4 cut(s) 89, 439, 595, 1087
MlyI GAGTC 6 cut(s) 38, 589, 610, 830, 1021, 1076
MmeI TCCRAC 2 cut(s) 444, 633
Mph1103I ATGCAT 1 cut(s) 942
MroI TCCGGA 2 cut(s) 401, 886
MseI TTAA 5 cut(s) 329, 500, 698, 882, 1061
MslI CAYNNNNRTG 1 cut(s) 522
MspI CCGG 7 cut(s) 65, 402, 675, 814, 826, 887, 1079
MspR9I CCNGG 2 cut(s) 676, 814
MwoI GCNNNNNNNGC 1 cut(s) 344
NciI CCSGG 2 cut(s) 676, 814
NdeI CATATG 1 cut(s) 1035
NdeII GATC 5 cut(s) 367, 474, 655, 685, 897
NlaIII CATG 5 cut(s) 322, 515, 563, 623, 940
NlaIV GGNNCC 4 cut(s) 310, 657, 830, 1077
NmuCI GTSAC 1 cut(s) 800
NsiI ATGCAT 1 cut(s) 942
NspI RCATGY 2 cut(s) 322, 563
NspV TTCGAA 1 cut(s) 189
PaeI GCATGC 1 cut(s) 322
PfeI GAWTC 6 cut(s) 122, 462, 527, 817, 915, 998
PflFI GACNNNGTC 1 cut(s) 839
PkrI GCNGC 1 cut(s) 140
PleI GAGTC 6 cut(s) 38, 588, 610, 830, 1020, 1075
PpsI GAGTC 6 cut(s) 38, 588, 610, 830, 1020, 1075
PsiI TTATAA 1 cut(s) 600
Psp124BI GAGCTC 1 cut(s) 935
PspEI GGTNACC 1 cut(s) 800
PspN4I GGNNCC 4 cut(s) 310, 657, 830, 1077
PsuI RGATCY 1 cut(s) 655
PsyI GACNNNGTC 1 cut(s) 839
RsaI GTAC 2 cut(s) 292, 744
RsaNI GTAC 2 cut(s) 291, 743
RseI CAYNNNNRTG 1 cut(s) 522
SacI GAGCTC 1 cut(s) 935
SaqAI TTAA 5 cut(s) 329, 500, 698, 882, 1061
SatI GCNGC 1 cut(s) 139
Sau3AI GATC 5 cut(s) 367, 474, 655, 685, 897
SchI GAGTC 6 cut(s) 38, 589, 610, 830, 1021, 1076
ScrFI CCNGG 2 cut(s) 676, 814
SduI GDGCHC 2 cut(s) 833, 935
SfaNI GCATC 1 cut(s) 150
SfuI TTCGAA 1 cut(s) 189
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 2 cut(s) 133, 703
SmoI CTYRAG 2 cut(s) 133, 703
SphI GCATGC 1 cut(s) 322
Sse9I AATT 4 cut(s) 89, 439, 595, 1087
SsiI CCGC 4 cut(s) 82, 213, 250, 397
SstI GAGCTC 1 cut(s) 935
StyD4I CCNGG 2 cut(s) 674, 812
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 3 cut(s) 426, 645, 765
TaqI TCGA 9 cut(s) 189, 477, 530, 589, 605, 626, 639, 739, 929
TasI AATT 4 cut(s) 89, 439, 595, 1087
TfiI GAWTC 6 cut(s) 122, 462, 527, 817, 915, 998
Tru1I TTAA 5 cut(s) 329, 500, 698, 882, 1061
Tru9I TTAA 5 cut(s) 329, 500, 698, 882, 1061
TscAI CASTG 2 cut(s) 236, 1077
TseFI GTSAC 1 cut(s) 800
TseI GCWGC 1 cut(s) 138
Tsp45I GTSAC 1 cut(s) 800
TspDTI ATGAA 7 cut(s) 438, 500, 506, 777, 784, 983, 1022
TspGWI ACGGA 6 cut(s) 340, 362, 374, 684, 1001, 1010
TspRI CASTG 2 cut(s) 236, 1077
Tth111I GACNNNGTC 1 cut(s) 839
XagI CCTNNNNNAGG 1 cut(s) 113
XceI RCATGY 2 cut(s) 322, 563
ZraI GACGTC 1 cut(s) 424
Zsp2I ATGCAT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.