MD14G1072700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012276
Physical Location & Seq
Reverse (-)
8091366 .. 8092901
1536 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1072700.v1.1.491

Sequence Viewer

Length: 1536 bp
ATGGGCTTCATAGCAAAAGCCAAGAGCAATAGCGATAGCAGCTGGAGCAGTGAAAATTGGGGGATGGGTTTGCTTCTGGTGTTCTTCCCGGAGGAAAACGGCGGCACCACCACCACCATTGTCGATAAAATCACCAAGCTCTTCTCTTCTTCTTCCACTTCCTCTCCATCCTCCTCCTCCTGTTCCTCCTCCAAAACATGCGCGGTTTTCAGAAGAACCAGCTCCAACAATCTTATTCTCTCCAAAGCCCAATCCACAATTTCCATCTGTGCCCTTCTCGTCTTCCTCACCCTCCTCCTCTTCACCCTCTCAACCTTCGAGCCCTCCCCCGCCAAGCCTCGCCCCGTCGTCCCATCACCTTCCAGAAGGTTCCTCTCCCAAAGTACTCAAATTCCCCAATCGCCCACCAATCACAACTTCCCCCTTCTCTCCTCCTCATGGTTGTCCGAAATGTGGAAGCAAAAACAGCAGAAGTTGTTTAAGTCCCAATTTGCCCTCCAGAAGATGGGCACTCTGTACCGCCGCGGCACCAGAGCCATGCCCGATCTCGTCGTCGCCCATTTCGACGACGACGTCACGGAACCCGAGCTCCGGTTGTTTCTGAGGGTGTTGCACCGGTCCGGCCTCACGTCCAGAGCGGACGTGGCGTTCGTCTTCGCCTCCTCCGGTTCGAGATTCGGGTCCATAATCCAGGAAGAGAACACCTCGTTCTTAGTACTCCTCCGCCGTTACGGGGGCCTAAACCGGACGGCTGCGGCGAGGGAAAAACCCGGTTTGGGATTCGACGCGGCTCAGTTCATCAAGGCCGGGAAAAAGGAAATTGAAGAGCCCCTCTGGGGGAAAAAGGGTGTAAACTCGGAAGGCGACGGTGAGTTGACTCGGTTTAGTTACGGGTCGGTTGTGGGGTTCGAGGCGGGCGAGCTGGACCCGGAAAACTCACTATCCGGATTCGTGGACCACGTAGTGCCGATGAGCCTGCGGCGGTGGGCTTGTTACCCGATGCTTCTCGGCCGGGTTCGAAGAAACTTCAAGCATATTATGTTGGTGGACGCGAAAAACTCGGTCGTGGTCAGGGACCCACTCGGCCGAGTCCGGCACCGGAGTCCCGAGTCGGTTCTTTTATTCACAATGCCGCCGCCGGGTAAGCACCCCAAGAAAAACTCCGACTCAACTCAGTCTCACGGCGCGGTTAATTCCGTCGTTATCACCGGCGGATCCCGCGGAATTCGGCGGCTATCGAACACCGTGTTGACGGAAATAGTTCGGGCCACCATGCAGCACAAGAGGAAGAGCATGGTGACCGAGTCGGGAATTTTGAGTCAACTCGTTGCCAACCCGTTCGTAAGGAAGAGCATTAATCTGGTCACGTCAAGTGAGTCGGTGCCGGACCCGAGTTCAATCGGCGGAGCAGCGGGGCTGTCCGGCGGGCACACAATTATCCAACGTGGTAATAGTAATCACGACGAGCTTAAGTCTATTATTATGAAGCAAATTTGTTTATTAGAAATAGATTCTTCTGTCTATAGGGATTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

512

Amino Acids

56.08

Weight (kDa)

9.89

Isoelectric Point (pI)

58.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7780 PF25002 163 - 460 3.9e-136 Domain of unknown function (DUF7780)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57400 AT5G52500
fragaria_vesca FvH4_6g23940
malus_domestica MD12G1074600.v1.1 MD14G1072700.v1.1
prunus_persica Prupe.7G058900_v2.0.a1
pyrus_communis pycom12g06420 pycom14g06260
rosa_chinensis RchiOBHm_Chr3g0482601
rosa_laevigata RLG00000023338
rosa_multiflora Rmu_sc0000224.1_g000003 Rmu_sc0004687.1_g000003
rosa_roxburghii Rroxscaffold_6G00399090
rosa_rugosa Rorug03G0197500
rosa_samantha Rh3AG248600 Rh3BG284100 Rh3CG281600 Rh3DG277800
rosa_wichuraiana Rw3G022450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 572
AatII GACGTC 1 cut(s) 576
AccB1I GGYRCC 4 cut(s) 104, 527, 1095, 1381
AccB7I CCANNNNNTGG 1 cut(s) 505
AccBSI CCGCTC 1 cut(s) 638
AccII CGCG 6 cut(s) 203, 525, 788, 1052, 1187, 1221
AccIII TCCGGA 1 cut(s) 944
AclWI GGATC 2 cut(s) 1209, 1222
AcoI YGGCCR 2 cut(s) 1009, 1084
AcsI RAATTY 4 cut(s) 390, 1224, 1311, 1491
AcyI GRCGYC 1 cut(s) 573
AdeI CACNNNGTG 1 cut(s) 964
AfaI GTAC 3 cut(s) 385, 518, 717
AfiI CCNNNNNNNGG 9 cut(s) 438, 453, 505, 591, 733, 776, 836, 837, 1139
AflII CTTAAG 1 cut(s) 1469
AgeI ACCGGT 1 cut(s) 615
AgsI TTSAA 3 cut(s) 824, 1030, 1398
AjiI CACGTC 3 cut(s) 630, 643, 1368
AjnI CCWGG 1 cut(s) 690
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AloI GAACNNNNNNTCC 4 cut(s) 573, 605, 632, 664
AluBI AGCT 6 cut(s) 42, 139, 222, 589, 922, 1468
AluI AGCT 6 cut(s) 42, 139, 222, 589, 922, 1468
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 1 cut(s) 1182
AlwI GGATC 2 cut(s) 1209, 1222
Ama87I CYCGRG 3 cut(s) 584, 1106, 1390
Aor13HI TCCGGA 1 cut(s) 944
AoxI GGCC 6 cut(s) 622, 736, 804, 1009, 1084, 1266
ApeKI GCWGC 4 cut(s) 39, 752, 1276, 1409
ApoI RAATTY 4 cut(s) 390, 1224, 1311, 1491
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AseI ATTAAT 1 cut(s) 1356
AsiGI ACCGGT 1 cut(s) 615
Asp700I GAANNNNTTC 1 cut(s) 1260
AspLEI GCGC 2 cut(s) 203, 1187
AspS9I GGNCC 8 cut(s) 618, 681, 736, 925, 955, 1075, 1266, 1387
AsuC2I CCSGG 6 cut(s) 89, 771, 808, 929, 1013, 1140
AsuHPI GGTGA 7 cut(s) 124, 280, 295, 348, 881, 1198, 1309
AsuII TTCGAA 1 cut(s) 1018
AvaI CYCGRG 3 cut(s) 584, 1106, 1390
AvaII GGWCC 6 cut(s) 618, 681, 925, 955, 1075, 1387
BaeGI GKGCMC 3 cut(s) 274, 512, 1431
BamHI GGATCC 1 cut(s) 1214
BanI GGYRCC 4 cut(s) 104, 527, 1095, 1381
BanII GRGCYC 3 cut(s) 324, 591, 831
BbsI GAAGAC 2 cut(s) 274, 646
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 4 cut(s) 51, 739, 1288, 1421
BccI CCATC 5 cut(s) 58, 175, 272, 361, 499
BceAI ACGGC 4 cut(s) 115, 711, 765, 1198
BcgI CGANNNNNNTGC 2 cut(s) 958, 992
BciT130I CCWGG 1 cut(s) 692
BcnI CCSGG 6 cut(s) 89, 771, 808, 929, 1013, 1140
BcoDI GTCTC 1 cut(s) 1182
BfmI CTRYAG 1 cut(s) 1522
BfrI CTTAAG 1 cut(s) 1469
BmcAI AGTACT 2 cut(s) 385, 717
Bme1390I CCNGG 7 cut(s) 89, 692, 771, 808, 929, 1013, 1140
Bme18I GGWCC 6 cut(s) 618, 681, 925, 955, 1075, 1387
BmeT110I CYCGRG 3 cut(s) 584, 1106, 1390
BmgBI CACGTC 3 cut(s) 630, 643, 1368
BmgT120I GGNCC 8 cut(s) 618, 681, 736, 925, 955, 1075, 1266, 1387
BmrFI CCNGG 7 cut(s) 89, 692, 771, 808, 929, 1013, 1140
BmsI GCATC 1 cut(s) 990
BpiI GAAGAC 2 cut(s) 274, 646
BplI GAGNNNNNCTC 2 cut(s) 689, 721
BpmI CTGGAG 2 cut(s) 64, 482
Bpu14I TTCGAA 1 cut(s) 1018
BpuMI CCSGG 6 cut(s) 89, 771, 808, 929, 1013, 1140
BsaAI YACGTR 1 cut(s) 961
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 2 cut(s) 523, 1219
BsaWI WCCGGW 6 cut(s) 591, 615, 665, 744, 944, 1098
BsaXI ACNNNNNCTCC 4 cut(s) 148, 178, 573, 603
Bsc4I CCNNNNNNNGG 9 cut(s) 438, 453, 505, 591, 733, 776, 836, 837, 1139
Bse118I RCCGGY 2 cut(s) 615, 1208
BseAI TCCGGA 1 cut(s) 944
BseBI CCWGG 1 cut(s) 692
BseDI CCNNGG 2 cut(s) 523, 1219
BseGI GGATG 2 cut(s) 69, 167
BseLI CCNNNNNNNGG 9 cut(s) 438, 453, 505, 591, 733, 776, 836, 837, 1139
BseMII CTCAG 3 cut(s) 593, 806, 1187
BseRI GAGGAG 9 cut(s) 163, 166, 178, 284, 287, 421, 424, 652, 710
BseSI GKGCMC 3 cut(s) 274, 512, 1431
BseX3I CGGCCG 2 cut(s) 1009, 1084
BseXI GCAGC 4 cut(s) 51, 739, 1288, 1421
Bsh1236I CGCG 6 cut(s) 203, 525, 788, 1052, 1187, 1221
Bsh1285I CGRYCG 3 cut(s) 1012, 1065, 1087
BshFI GGCC 6 cut(s) 624, 738, 806, 1011, 1086, 1268
BshNI GGYRCC 4 cut(s) 104, 527, 1095, 1381
BshTI ACCGGT 1 cut(s) 615
BsiEI CGRYCG 3 cut(s) 1012, 1065, 1087
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 3 cut(s) 584, 1106, 1390
BslFI GGGAC 4 cut(s) 335, 469, 1088, 1089
BslI CCNNNNNNNGG 9 cut(s) 438, 453, 505, 591, 733, 776, 836, 837, 1139
BsmAI GTCTC 1 cut(s) 1182
BsmFI GGGAC 4 cut(s) 335, 469, 1088, 1089
BsnI GGCC 6 cut(s) 624, 738, 806, 1011, 1086, 1268
BsoBI CYCGRG 3 cut(s) 584, 1106, 1390
Bsp119I TTCGAA 1 cut(s) 1018
Bsp1286I GDGCHC 6 cut(s) 274, 324, 512, 591, 831, 1431
Bsp13I TCCGGA 1 cut(s) 944
Bsp143I GATC 2 cut(s) 544, 1214
BspANI GGCC 6 cut(s) 624, 738, 806, 1011, 1086, 1268
BspCNI CTCAG 3 cut(s) 594, 805, 1186
BspEI TCCGGA 1 cut(s) 944
BspFNI CGCG 6 cut(s) 203, 525, 788, 1052, 1187, 1221
BspPI GGATC 2 cut(s) 1209, 1222
BspQI GCTCTTC 4 cut(s) 146, 819, 1283, 1343
BspT104I TTCGAA 1 cut(s) 1018
BspT107I GGYRCC 4 cut(s) 104, 527, 1095, 1381
BspTI CTTAAG 1 cut(s) 1469
BsrBI CCGCTC 1 cut(s) 638
BsrFI RCCGGY 2 cut(s) 615, 1208
BssAI RCCGGY 2 cut(s) 615, 1208
BssECI CCNNGG 2 cut(s) 523, 1219
BssMI GATC 2 cut(s) 544, 1214
BssNI GRCGYC 1 cut(s) 573
Bst2UI CCWGG 1 cut(s) 692
Bst4CI ACNGT 2 cut(s) 869, 1246
Bst6I CTCTTC 7 cut(s) 146, 151, 305, 690, 819, 1283, 1343
BstACI GRCGYC 1 cut(s) 573
BstAFI CTTAAG 1 cut(s) 1469
BstBAI YACGTR 1 cut(s) 961
BstBI TTCGAA 1 cut(s) 1018
BstC8I GCNNGC 4 cut(s) 916, 920, 977, 1427
BstDEI CTNAG 4 cut(s) 602, 712, 792, 1173
BstDSI CCRYGG 2 cut(s) 523, 1219
BstEII GGTNACC 1 cut(s) 1297
BstF5I GGATG 2 cut(s) 69, 167
BstFNI CGCG 6 cut(s) 203, 525, 788, 1052, 1187, 1221
BstHHI GCGC 2 cut(s) 203, 1187
BstKTI GATC 2 cut(s) 547, 1217
BstMAI GTCTC 1 cut(s) 1182
BstMBI GATC 2 cut(s) 544, 1214
BstMCI CGRYCG 3 cut(s) 1012, 1065, 1087
BstMWI GCNNNNNNNGC 6 cut(s) 39, 45, 466, 644, 1144, 1218
BstNI CCWGG 1 cut(s) 692
BstNSI RCATGY 1 cut(s) 201
BstPI GGTNACC 1 cut(s) 1297
BstSCI CCNGG 7 cut(s) 87, 690, 769, 806, 927, 1011, 1138
BstSFI CTRYAG 1 cut(s) 1522
BstSLI GKGCMC 3 cut(s) 274, 512, 1431
BstUI CGCG 6 cut(s) 203, 525, 788, 1052, 1187, 1221
BstV1I GCAGC 4 cut(s) 51, 739, 1288, 1421
BstV2I GAAGAC 2 cut(s) 274, 646
BstX2I RGATCY 1 cut(s) 1214
BstYI RGATCY 1 cut(s) 1214
BstZI CGGCCG 2 cut(s) 1009, 1084
BsuRI GGCC 6 cut(s) 624, 738, 806, 1011, 1086, 1268
BtgI CCRYGG 2 cut(s) 523, 1219
BtrI CACGTC 3 cut(s) 630, 643, 1368
BtsCI GGATG 2 cut(s) 69, 167
BtsI GCAGTG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 4 cut(s) 916, 920, 977, 1427
CfoI GCGC 2 cut(s) 203, 1187
Cfr10I RCCGGY 2 cut(s) 615, 1208
Cfr13I GGNCC 8 cut(s) 618, 681, 736, 925, 955, 1075, 1266, 1387
Cfr42I CCGCGG 2 cut(s) 526, 1222
CpoI CGGWCCG 1 cut(s) 618
CseI GACGC 2 cut(s) 794, 1058
Csp6I GTAC 3 cut(s) 384, 517, 716
CspAI ACCGGT 1 cut(s) 615
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CspI CGGWCCG 1 cut(s) 618
CviAII CATG 5 cut(s) 198, 438, 538, 1273, 1294
CviQI GTAC 3 cut(s) 384, 517, 716
DdeI CTNAG 4 cut(s) 602, 712, 792, 1173
DpnI GATC 2 cut(s) 546, 1216
DpnII GATC 2 cut(s) 544, 1214
DraIII CACNNNGTG 1 cut(s) 964
DrdI GACNNNNNNGTC 1 cut(s) 572
DseDI GACNNNNNNGTC 1 cut(s) 572
EaeI YGGCCR 2 cut(s) 1009, 1084
EagI CGGCCG 2 cut(s) 1009, 1084
Eam1104I CTCTTC 7 cut(s) 146, 151, 305, 690, 819, 1283, 1343
EarI CTCTTC 7 cut(s) 146, 151, 305, 690, 819, 1283, 1343
EciI GGCGGA 3 cut(s) 713, 1227, 1419
Ecl136II GAGCTC 1 cut(s) 589
EclXI CGGCCG 2 cut(s) 1009, 1084
Eco24I GRGCYC 3 cut(s) 324, 591, 831
Eco47I GGWCC 6 cut(s) 618, 681, 925, 955, 1075, 1387
Eco52I CGGCCG 2 cut(s) 1009, 1084
Eco53kI GAGCTC 1 cut(s) 589
Eco88I CYCGRG 3 cut(s) 584, 1106, 1390
Eco91I GGTNACC 1 cut(s) 1297
EcoICRI GAGCTC 1 cut(s) 589
EcoO109I RGGNCCY 2 cut(s) 736, 1075
EcoO65I GGTNACC 1 cut(s) 1297
EcoRI GAATTC 1 cut(s) 1224
EcoRII CCWGG 1 cut(s) 690
EcoT38I GRGCYC 3 cut(s) 324, 591, 831
FaeI CATG 5 cut(s) 201, 441, 541, 1276, 1297
FaqI GGGAC 4 cut(s) 335, 469, 1088, 1089
FatI CATG 5 cut(s) 197, 437, 537, 1272, 1293
FauI CCCGC 5 cut(s) 337, 907, 1226, 1405, 1418
FokI GGATG 2 cut(s) 76, 154
FriOI GRGCYC 3 cut(s) 324, 591, 831
GlaI GCGC 2 cut(s) 202, 1186
GsuI CTGGAG 2 cut(s) 64, 482
HaeIII GGCC 6 cut(s) 624, 738, 806, 1011, 1086, 1268
HgaI GACGC 2 cut(s) 794, 1058
HhaI GCGC 2 cut(s) 203, 1187
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 5 cut(s) 201, 441, 541, 1276, 1297
Hin6I GCGC 2 cut(s) 201, 1185
HinP1I GCGC 2 cut(s) 201, 1185
HincII GTYRAC 3 cut(s) 876, 1251, 1322
HindII GTYRAC 3 cut(s) 876, 1251, 1322
HphI GGTGA 7 cut(s) 124, 280, 295, 348, 881, 1198, 1309
Hpy166II GTNNAC 6 cut(s) 853, 876, 955, 1048, 1251, 1322
Hpy188I TCNGA 5 cut(s) 212, 448, 603, 859, 1165
Hpy188III TCNNGA 8 cut(s) 363, 499, 633, 672, 945, 1106, 1308, 1460
Hpy8I GTNNAC 6 cut(s) 853, 876, 955, 1048, 1251, 1322
HpyAV CCTTC 6 cut(s) 284, 325, 360, 369, 434, 854
HpyCH4III ACNGT 2 cut(s) 869, 1246
HpyCH4IV ACGT 6 cut(s) 573, 629, 642, 960, 1367, 1444
HpyCH4V TGCA 2 cut(s) 613, 1276
HpyF10VI GCNNNNNNNGC 6 cut(s) 39, 45, 466, 644, 1144, 1218
HpyF3I CTNAG 4 cut(s) 602, 712, 792, 1173
HpySE526I ACGT 6 cut(s) 573, 629, 642, 960, 1367, 1444
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 5 cut(s) 201, 441, 541, 1276, 1297
HspAI GCGC 2 cut(s) 201, 1185
KflI GGGWCCC 1 cut(s) 1075
Kpn2I TCCGGA 1 cut(s) 944
KspI CCGCGG 2 cut(s) 526, 1222
Kzo9I GATC 2 cut(s) 544, 1214
LguI GCTCTTC 4 cut(s) 146, 819, 1283, 1343
LmnI GCTCC 4 cut(s) 45, 227, 594, 1406
Lsp1109I GCAGC 4 cut(s) 51, 739, 1288, 1421
LweI GCATC 1 cut(s) 990
MaeII ACGT 6 cut(s) 573, 629, 642, 960, 1367, 1444
MaeIII GTNAC 6 cut(s) 574, 728, 887, 992, 1297, 1363
MalI GATC 2 cut(s) 546, 1216
MbiI CCGCTC 1 cut(s) 638
MboI GATC 2 cut(s) 544, 1214
MflI RGATCY 1 cut(s) 1214
MhlI GDGCHC 6 cut(s) 274, 324, 512, 591, 831, 1431
MlyI GAGTC 8 cut(s) 871, 1098, 1111, 1118, 1160, 1313, 1327, 1385
MmeI TCCRAC 3 cut(s) 249, 1188, 1465
MroI TCCGGA 1 cut(s) 944
MroXI GAANNNNTTC 1 cut(s) 1260
MseI TTAA 4 cut(s) 480, 1191, 1356, 1470
MspA1I CMGCKG 4 cut(s) 42, 525, 1221, 1412
MspCI CTTAAG 1 cut(s) 1469
MspR9I CCNGG 7 cut(s) 89, 692, 771, 808, 929, 1013, 1140
MvaI CCWGG 1 cut(s) 692
MvnI CGCG 6 cut(s) 203, 525, 788, 1052, 1187, 1221
MwoI GCNNNNNNNGC 6 cut(s) 39, 45, 466, 644, 1144, 1218
NciI CCSGG 6 cut(s) 89, 771, 808, 929, 1013, 1140
NdeII GATC 2 cut(s) 544, 1214
NlaIII CATG 5 cut(s) 201, 441, 541, 1276, 1297
NmeAIII GCCGAG 3 cut(s) 987, 1062, 1112
NmuCI GTSAC 3 cut(s) 574, 1297, 1363
NspI RCATGY 1 cut(s) 201
NspV TTCGAA 1 cut(s) 1018
PciSI GCTCTTC 4 cut(s) 146, 819, 1283, 1343
PcsI WCGNNNNNNNCGW 6 cut(s) 561, 570, 648, 755, 915, 957
PdmI GAANNNNTTC 1 cut(s) 1260
PfeI GAWTC 4 cut(s) 675, 780, 948, 1511
PflFI GACNNNGTC 2 cut(s) 572, 1303
PflMI CCANNNNNTGG 1 cut(s) 505
PfoI TCCNGGA 2 cut(s) 87, 690
PinAI ACCGGT 1 cut(s) 615
PleI GAGTC 8 cut(s) 871, 1097, 1110, 1117, 1160, 1312, 1326, 1384
PpsI GAGTC 8 cut(s) 871, 1097, 1110, 1117, 1160, 1312, 1326, 1384
Ppu21I YACGTR 1 cut(s) 961
PpuMI RGGWCCY 1 cut(s) 1075
PshBI ATTAAT 1 cut(s) 1356
Psp124BI GAGCTC 1 cut(s) 591
Psp5II RGGWCCY 1 cut(s) 1075
Psp6I CCWGG 1 cut(s) 690
PspEI GGTNACC 1 cut(s) 1297
PspGI CCWGG 1 cut(s) 690
PspPI GGNCC 8 cut(s) 618, 681, 736, 925, 955, 1075, 1266, 1387
PspPPI RGGWCCY 1 cut(s) 1075
PsuI RGATCY 1 cut(s) 1214
PsyI GACNNNGTC 2 cut(s) 572, 1303
PvuII CAGCTG 1 cut(s) 42
RsaI GTAC 3 cut(s) 385, 518, 717
RsaNI GTAC 3 cut(s) 384, 517, 716
Rsr2I CGGWCCG 1 cut(s) 618
RsrII CGGWCCG 1 cut(s) 618
SacI GAGCTC 1 cut(s) 591
SacII CCGCGG 2 cut(s) 526, 1222
SapI GCTCTTC 4 cut(s) 146, 819, 1283, 1343
SaqAI TTAA 4 cut(s) 480, 1191, 1356, 1470
Sau3AI GATC 2 cut(s) 544, 1214
Sau96I GGNCC 8 cut(s) 618, 681, 736, 925, 955, 1075, 1266, 1387
ScaI AGTACT 2 cut(s) 385, 717
SchI GAGTC 8 cut(s) 871, 1098, 1111, 1118, 1160, 1313, 1327, 1385
ScrFI CCNGG 7 cut(s) 89, 692, 771, 808, 929, 1013, 1140
SduI GDGCHC 6 cut(s) 274, 324, 512, 591, 831, 1431
SfaNI GCATC 1 cut(s) 990
SfcI CTRYAG 1 cut(s) 1522
Sfr303I CCGCGG 2 cut(s) 526, 1222
SfuI TTCGAA 1 cut(s) 1018
SgrAI CRCCGGYG 1 cut(s) 1208
SgrBI CCGCGG 2 cut(s) 526, 1222
SinI GGWCC 6 cut(s) 618, 681, 925, 955, 1075, 1387
SmlI CTYRAG 1 cut(s) 1469
SmoI CTYRAG 1 cut(s) 1469
SstI GAGCTC 1 cut(s) 591
StyD4I CCNGG 7 cut(s) 87, 690, 769, 806, 927, 1011, 1138
TaaI ACNGT 2 cut(s) 869, 1246
TaiI ACGT 6 cut(s) 576, 632, 645, 963, 1370, 1447
TaqI TCGA 8 cut(s) 123, 318, 564, 671, 783, 909, 1018, 1238
TaqII GACCGA 2 cut(s) 1051, 1316
TatI WGTACW 2 cut(s) 383, 715
TauI GCSGC 9 cut(s) 105, 525, 528, 758, 791, 982, 1135, 1138, 1234
TfiI GAWTC 4 cut(s) 675, 780, 948, 1511
Tru1I TTAA 4 cut(s) 480, 1191, 1356, 1470
Tru9I TTAA 4 cut(s) 480, 1191, 1356, 1470
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 3 cut(s) 574, 1297, 1363
TseI GCWGC 4 cut(s) 39, 752, 1276, 1409
Tsp45I GTSAC 3 cut(s) 574, 1297, 1363
TspDTI ATGAA 2 cut(s) 787, 1499
TspGWI ACGGA 3 cut(s) 593, 1186, 1268
TspRI CASTG 1 cut(s) 55
Tth111I GACNNNGTC 2 cut(s) 572, 1303
Van91I CCANNNNNTGG 1 cut(s) 505
Vha464I CTTAAG 1 cut(s) 1469
VpaK11BI GGWCC 6 cut(s) 618, 681, 925, 955, 1075, 1387
VspI ATTAAT 1 cut(s) 1356
XapI RAATTY 4 cut(s) 390, 1224, 1311, 1491
XceI RCATGY 1 cut(s) 201
XcmI CCANNNNNNNNNTGG 1 cut(s) 640
XmnI GAANNNNTTC 1 cut(s) 1260
ZraI GACGTC 1 cut(s) 574
ZrmI AGTACT 2 cut(s) 385, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.