AT5G52540

Protein of unknown function (DUF819)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009936
Physical Location & Seq
Reverse (-)
21321428 .. 21323780
2353 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52540.1

Sequence Viewer

Length: 1386 bp
ATGGCGACGAGCTTGCCATTACTCAATGTGATCGGAAACTCGTTACCGTCGTCGTCGAGACTATTTCGTCGGCGTTCGTACATTGTTTCACGCCAAGATCTACCTGAATTCTCAGCTAAATCAACCTCTTCAAGGCTTAGCTTCTCTCCTGCTTCGTTGTCGCAATTTCGTAACGGAACCGACAGGTTTCTGTCGCCGGAATCATCATCTCCGATCCGATCGGTTACTGTGAGTTCGTTGAGTACGCCTCTCATTTCTCCCAACGATGAATGGGGAACTTGGACTGCTCTCTTCGCTACTGGTGCTCTCGGTCTCTGGTCGGAGAAGACAAAGGTCGGAGCCGCTATGAGTGGTGCGTTAGTCAGCACTCTAGTTGGTCTTGCAGCTAGTAATCTAGGGATCATTTCTTCTCAAGCTCCTGCTTTTGCTGTTGTGTTGAATTTTCTGCTTCCATTAGCTGTTCCTCTGTTGTTGTTTAGAGCTGATTTGCGTCGTGTGGTTCAGTCCACTGGAAAGCTTCTCTTGGCCTTTTTGATTGGATCAGTTGCCACAACGGTGGGTACAGCTCTGGCATACTATTTAGTGCCAATGAAGTCACTTGGCCCTGATAGTTGGAAGATTGCAGCTGCTCTCATGGGAAGACATATCGGTGGAGCCGTCAACTACGTAGCTATATCAAATGCTCTTGGAGTTACACCATCGGTTTTAGCTGCTGGACTGGCTGCTGATAATGTTATATGCGCTGTATATTTCACGACATTGTTTGCTTTGGGCTCCAAAATACCTGCAGAAGCTGTACCGCCACCGACTACTATTGTAGATGCAGAGACGAACGAAGCTTCTGAAACTAAAAATAAAATTCCTGTGCTACTGATAGCTACTGGAATTGCTGTGTCATTAGCTATCTGCAAGGCTGGGGCTTTGCTAACAAAGTACTTCGGAATTTCGGGTGGTAGCTTACCGGCTATAACAGCGGTTGTTGTTATTTTAGCAACTGTCTTTCCGTCCCAGTTTGGTCGTCTTGCTCCATCTGGAGAAGCCATGGCTCTAATTTTGATGCAGGTGTTCTTCACAGTGGTGGGTGCAAGCGGAAACATATGGAGTGTGATCAACACGGCGCCGAGCATATTCTTGTTTGCATTGGTTCAAATTGGGACACATCTGGCTGTGATATTGGGCATAGGAAAGCTGCTAAACATCGAGCTAAGGTTACTGCTTCTAGCATCAAATGCTAATGTCGGAGGACCCACGACCGCAGCTGGAATGGCGACGGCAAAGGGATGGAACTCGTTGATAGTTCCCGGGATTCTGGCCGGAATATTTGGGATCGCCATTGCAACTTTCATAGGAATTGCATTTGGAGTTAAAGTTCTTAAGTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF819

Protein Analysis

461

Amino Acids

47.64

Weight (kDa)

9.96

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF819 PF05684 95 - 460 1.3e-133 Protein of unknown function (DUF819)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1051
AasI GACNNNNNNGTC 1 cut(s) 66
Acc36I ACCTGC 2 cut(s) 793, 1051
AccB1I GGYRCC 1 cut(s) 1117
AciI CCGC 5 cut(s) 342, 800, 974, 1089, 1254
AclWI GGATC 4 cut(s) 208, 407, 547, 1334
AcoI YGGCCR 1 cut(s) 1311
AcsI RAATTY 4 cut(s) 107, 439, 858, 942
AcyI GRCGYC 1 cut(s) 1118
AfaI GTAC 5 cut(s) 80, 244, 562, 798, 935
AfiI CCNNNNNNNGG 1 cut(s) 132
AflII CTTAAG 1 cut(s) 1373
AgsI TTSAA 3 cut(s) 132, 439, 1148
AleI CACNNNNGTG 2 cut(s) 554, 1076
AloI GAACNNNNNNTCC 2 cut(s) 1353, 1385
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 3 cut(s) 52, 317, 821
AlwI GGATC 4 cut(s) 208, 407, 547, 1334
AlwNI CAGNNNCTG 1 cut(s) 794
Ama87I CYCGRG 1 cut(s) 1301
AoxI GGCC 3 cut(s) 525, 601, 1311
ApeKI GCWGC 7 cut(s) 383, 623, 626, 710, 722, 1189, 1256
ApoI RAATTY 4 cut(s) 107, 439, 858, 942
AspLEI GCGC 2 cut(s) 743, 1120
AspS9I GGNCC 2 cut(s) 602, 1244
AsuC2I CCSGG 2 cut(s) 1302, 1303
AvaI CYCGRG 1 cut(s) 1301
AvaII GGWCC 1 cut(s) 1244
BanI GGYRCC 1 cut(s) 1117
BanII GRGCYC 1 cut(s) 776
BbsI GAAGAC 2 cut(s) 332, 646
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 7 cut(s) 395, 613, 635, 697, 709, 1176, 1268
BccI CCATC 3 cut(s) 706, 1036, 1275
BceAI ACGGC 3 cut(s) 641, 1131, 1287
BcgI CGANNNNNNTGC 1 cut(s) 29
BclI TGATCA 1 cut(s) 1107
BcnI CCSGG 2 cut(s) 1302, 1303
BcoDI GTCTC 3 cut(s) 52, 317, 821
BfaI CTAG 4 cut(s) 371, 387, 395, 1220
BfmI CTRYAG 1 cut(s) 786
BfoI RGCGCY 1 cut(s) 1121
BfrI CTTAAG 1 cut(s) 1373
BfuAI ACCTGC 2 cut(s) 793, 1051
BglII AGATCT 1 cut(s) 97
BisI GCNGC 8 cut(s) 342, 384, 624, 627, 711, 723, 1190, 1257
BlpI GCTNAGC 1 cut(s) 137
BlsI GCNGC 8 cut(s) 343, 385, 625, 628, 712, 724, 1191, 1258
BmcAI AGTACT 1 cut(s) 935
Bme1390I CCNGG 2 cut(s) 1302, 1303
Bme18I GGWCC 1 cut(s) 1244
BmeT110I CYCGRG 1 cut(s) 1301
BmgT120I GGNCC 2 cut(s) 602, 1244
BmiI GGNNCC 6 cut(s) 178, 340, 655, 775, 1119, 1246
BmrFI CCNGG 2 cut(s) 1302, 1303
BmrI ACTGGG 1 cut(s) 1003
BmsI GCATC 3 cut(s) 811, 1047, 1232
BmuI ACTGGG 1 cut(s) 1003
BoxI GACNNNNGTC 1 cut(s) 332
BpiI GAAGAC 2 cut(s) 332, 646
BplI GAGNNNNNCTC 2 cut(s) 232, 264
BpmI CTGGAG 1 cut(s) 1053
Bpu10I CCTNAGC 1 cut(s) 1205
Bpu1102I GCTNAGC 1 cut(s) 137
BpuEI CTTGAG 1 cut(s) 396
BpuMI CCSGG 2 cut(s) 1302, 1303
BsaAI YACGTR 1 cut(s) 667
BsaHI GRCGYC 1 cut(s) 1118
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 1041, 1301
BsaXI ACNNNNNCTCC 2 cut(s) 1353, 1383
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse118I RCCGGY 1 cut(s) 961
Bse1I ACTGG 5 cut(s) 304, 514, 723, 886, 1009
Bse3DI GCAATG 1 cut(s) 1332
BseDI CCNNGG 2 cut(s) 1041, 1301
BseGI GGATG 1 cut(s) 1286
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMI GCAATG 1 cut(s) 1332
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 5 cut(s) 304, 514, 723, 886, 1009
BseXI GCAGC 7 cut(s) 395, 613, 635, 697, 709, 1176, 1268
BseYI CCCAGC 1 cut(s) 914
Bsh1285I CGRYCG 2 cut(s) 221, 1254
BshFI GGCC 3 cut(s) 527, 603, 1313
BshNI GGYRCC 1 cut(s) 1117
BsiEI CGRYCG 2 cut(s) 221, 1254
BsiHKAI GWGCWC 1 cut(s) 307
BsiHKCI CYCGRG 1 cut(s) 1301
BsiSI CCGG 4 cut(s) 197, 962, 1302, 1314
BslFI GGGAC 2 cut(s) 991, 1168
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 3 cut(s) 52, 317, 821
BsmBI CGTCTC 1 cut(s) 821
BsmFI GGGAC 2 cut(s) 991, 1168
BsnI GGCC 3 cut(s) 527, 603, 1313
Bso31I GGTCTC 1 cut(s) 317
BsoBI CYCGRG 1 cut(s) 1301
Bsp1286I GDGCHC 2 cut(s) 307, 776
Bsp143I GATC 8 cut(s) 30, 97, 213, 218, 399, 539, 1107, 1326
Bsp1720I GCTNAGC 1 cut(s) 137
Bsp19I CCATGG 1 cut(s) 1041
BspACI CCGC 5 cut(s) 342, 800, 974, 1089, 1254
BspANI GGCC 3 cut(s) 527, 603, 1313
BspCNI CTCAG 1 cut(s) 125
BspLI GGNNCC 6 cut(s) 178, 340, 655, 775, 1119, 1246
BspMAI CTGCAG 1 cut(s) 790
BspMI ACCTGC 2 cut(s) 793, 1051
BspPI GGATC 4 cut(s) 208, 407, 547, 1334
BspT107I GGYRCC 1 cut(s) 1117
BspTI CTTAAG 1 cut(s) 1373
BspTNI GGTCTC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 1332
BsrFI RCCGGY 1 cut(s) 961
BsrI ACTGG 5 cut(s) 304, 514, 723, 886, 1009
BssAI RCCGGY 1 cut(s) 961
BssECI CCNNGG 2 cut(s) 1041, 1301
BssMI GATC 8 cut(s) 30, 97, 213, 218, 399, 539, 1107, 1326
BssNI GRCGYC 1 cut(s) 1118
BssT1I CCWWGG 1 cut(s) 1041
Bst4CI ACNGT 5 cut(s) 48, 229, 556, 997, 1075
Bst6I CTCTTC 2 cut(s) 133, 296
BstACI GRCGYC 1 cut(s) 1118
BstAFI CTTAAG 1 cut(s) 1373
BstAPI GCANNNNNTGC 1 cut(s) 1229
BstBAI YACGTR 1 cut(s) 667
BstC8I GCNNGC 2 cut(s) 14, 1087
BstDEI CTNAG 3 cut(s) 112, 137, 1205
BstDSI CCRYGG 1 cut(s) 1041
BstENI CCTNNNNNAGG 1 cut(s) 130
BstF5I GGATG 1 cut(s) 1286
BstH2I RGCGCY 1 cut(s) 1121
BstHHI GCGC 2 cut(s) 743, 1120
BstKTI GATC 8 cut(s) 33, 100, 216, 221, 402, 542, 1110, 1329
BstMAI GTCTC 3 cut(s) 52, 317, 821
BstMBI GATC 8 cut(s) 30, 97, 213, 218, 399, 539, 1107, 1326
BstMCI CGRYCG 2 cut(s) 221, 1254
BstMWI GCNNNNNNNGC 6 cut(s) 293, 302, 719, 971, 1229, 1265
BstPAI GACNNNNGTC 1 cut(s) 332
BstSCI CCNGG 2 cut(s) 1300, 1301
BstSFI CTRYAG 1 cut(s) 786
BstSNI TACGTA 1 cut(s) 667
BstV1I GCAGC 7 cut(s) 395, 613, 635, 697, 709, 1176, 1268
BstV2I GAAGAC 2 cut(s) 332, 646
BstX2I RGATCY 1 cut(s) 97
BstXI CCANNNNNNTGG 1 cut(s) 556
BstYI RGATCY 1 cut(s) 97
BsuRI GGCC 3 cut(s) 527, 603, 1313
BtgI CCRYGG 1 cut(s) 1041
BtsCI GGATG 1 cut(s) 1286
BtsIMutI CAGTG 2 cut(s) 507, 1080
BveI ACCTGC 2 cut(s) 793, 1051
Cac8I GCNNGC 2 cut(s) 14, 1087
CaiI CAGNNNCTG 1 cut(s) 794
CfoI GCGC 2 cut(s) 743, 1120
Cfr10I RCCGGY 1 cut(s) 961
Cfr13I GGNCC 2 cut(s) 602, 1244
Cfr9I CCCGGG 1 cut(s) 1301
CseI GACGC 1 cut(s) 479
Csp6I GTAC 5 cut(s) 79, 243, 561, 797, 934
CviAII CATG 3 cut(s) 634, 1042, 1381
CviQI GTAC 5 cut(s) 79, 243, 561, 797, 934
DdeI CTNAG 3 cut(s) 112, 137, 1205
DinI GGCGCC 1 cut(s) 1119
DpnI GATC 8 cut(s) 32, 99, 215, 220, 401, 541, 1109, 1328
DpnII GATC 8 cut(s) 30, 97, 213, 218, 399, 539, 1107, 1326
DrdI GACNNNNNNGTC 1 cut(s) 66
DseDI GACNNNNNNGTC 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 1311
Eam1104I CTCTTC 2 cut(s) 133, 296
EarI CTCTTC 2 cut(s) 133, 296
Eco105I TACGTA 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 1041
Eco24I GRGCYC 1 cut(s) 776
Eco31I GGTCTC 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 1244
Eco88I CYCGRG 1 cut(s) 1301
EcoNI CCTNNNNNAGG 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 1244
EcoRI GAATTC 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 1041
EcoT38I GRGCYC 1 cut(s) 776
EgeI GGCGCC 1 cut(s) 1119
EheI GGCGCC 1 cut(s) 1119
ErhI CCWWGG 1 cut(s) 1041
Esp3I CGTCTC 1 cut(s) 821
FaeI CATG 3 cut(s) 637, 1045, 1384
FalI AAGNNNNNCTT 2 cut(s) 506, 538
FaqI GGGAC 2 cut(s) 991, 1168
FatI CATG 3 cut(s) 633, 1041, 1380
FauNDI CATATG 1 cut(s) 1097
FbaI TGATCA 1 cut(s) 1107
Fnu4HI GCNGC 8 cut(s) 342, 384, 624, 627, 711, 723, 1190, 1257
FokI GGATG 1 cut(s) 1293
FriOI GRGCYC 1 cut(s) 776
Fsp4HI GCNGC 8 cut(s) 342, 384, 624, 627, 711, 723, 1190, 1257
FspBI CTAG 4 cut(s) 371, 387, 395, 1220
GlaI GCGC 2 cut(s) 742, 1119
GluI GCNGC 8 cut(s) 342, 384, 624, 627, 711, 723, 1190, 1257
GsaI CCCAGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 1053
HaeII RGCGCY 1 cut(s) 1121
HaeIII GGCC 3 cut(s) 527, 603, 1313
HapII CCGG 4 cut(s) 197, 962, 1302, 1314
HgaI GACGC 1 cut(s) 479
HhaI GCGC 2 cut(s) 743, 1120
Hin1I GRCGYC 1 cut(s) 1118
Hin1II CATG 3 cut(s) 637, 1045, 1384
Hin6I GCGC 2 cut(s) 741, 1118
HinP1I GCGC 2 cut(s) 741, 1118
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HindIII AAGCTT 2 cut(s) 515, 837
HinfI GANTC 2 cut(s) 200, 1306
HpaII CCGG 4 cut(s) 197, 962, 1302, 1314
Hpy166II GTNNAC 2 cut(s) 507, 661
Hpy188I TCNGA 8 cut(s) 35, 213, 218, 322, 338, 844, 941, 1241
Hpy188III TCNNGA 3 cut(s) 57, 754, 1032
Hpy8I GTNNAC 2 cut(s) 507, 661
Hpy99I CGWCG 7 cut(s) 10, 52, 55, 58, 72, 495, 1273
HpyCH4III ACNGT 5 cut(s) 48, 229, 556, 997, 1075
HpyCH4IV ACGT 1 cut(s) 666
HpyF10VI GCNNNNNNNGC 6 cut(s) 293, 302, 719, 971, 1229, 1265
HpyF3I CTNAG 3 cut(s) 112, 137, 1205
HpySE526I ACGT 1 cut(s) 666
Hsp92I GRCGYC 1 cut(s) 1118
Hsp92II CATG 3 cut(s) 637, 1045, 1384
HspAI GCGC 2 cut(s) 741, 1118
KasI GGCGCC 1 cut(s) 1117
Ksp22I TGATCA 1 cut(s) 1107
Kzo9I GATC 8 cut(s) 30, 97, 213, 218, 399, 539, 1107, 1326
LmnI GCTCC 5 cut(s) 338, 421, 653, 779, 1030
Lsp1109I GCAGC 7 cut(s) 395, 613, 635, 697, 709, 1176, 1268
LweI GCATC 3 cut(s) 811, 1047, 1232
MaeI CTAG 4 cut(s) 371, 387, 395, 1220
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 6 cut(s) 42, 170, 223, 594, 691, 1209
MalI GATC 8 cut(s) 32, 99, 215, 220, 401, 541, 1109, 1328
MboI GATC 8 cut(s) 30, 97, 213, 218, 399, 539, 1107, 1326
MboII GAAGA 7 cut(s) 120, 283, 337, 399, 628, 651, 1060
MflI RGATCY 1 cut(s) 97
MhlI GDGCHC 2 cut(s) 307, 776
MluCI AATT 9 cut(s) 107, 164, 439, 858, 885, 942, 1050, 1149, 1350
Mly113I GGCGCC 1 cut(s) 1118
MmeI TCCRAC 4 cut(s) 300, 316, 593, 1219
MnlI CCTC 4 cut(s) 136, 258, 474, 1235
MseI TTAA 2 cut(s) 1365, 1374
MslI CAYNNNNRTG 3 cut(s) 554, 648, 1076
MspA1I CMGCKG 3 cut(s) 626, 974, 1259
MspCI CTTAAG 1 cut(s) 1373
MspI CCGG 4 cut(s) 197, 962, 1302, 1314
MspR9I CCNGG 2 cut(s) 1302, 1303
MwoI GCNNNNNNNGC 6 cut(s) 293, 302, 719, 971, 1229, 1265
NarI GGCGCC 1 cut(s) 1118
NciI CCSGG 2 cut(s) 1302, 1303
NcoI CCATGG 1 cut(s) 1041
NdeI CATATG 1 cut(s) 1097
NdeII GATC 8 cut(s) 30, 97, 213, 218, 399, 539, 1107, 1326
NlaIII CATG 3 cut(s) 637, 1045, 1384
NlaIV GGNNCC 6 cut(s) 178, 340, 655, 775, 1119, 1246
NmeAIII GCCGAG 1 cut(s) 1146
NmuCI GTSAC 1 cut(s) 594
OliI CACNNNNGTG 2 cut(s) 554, 1076
PaqCI CACCTGC 1 cut(s) 1051
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 654
PfeI GAWTC 2 cut(s) 200, 1306
PkrI GCNGC 8 cut(s) 343, 385, 625, 628, 712, 724, 1191, 1258
Ple19I CGATCG 1 cut(s) 221
PluTI GGCGCC 1 cut(s) 1121
Ppu21I YACGTR 1 cut(s) 667
PpuMI RGGWCCY 1 cut(s) 1244
PshAI GACNNNNGTC 1 cut(s) 332
Psp5II RGGWCCY 1 cut(s) 1244
PspFI CCCAGC 1 cut(s) 914
PspN4I GGNNCC 6 cut(s) 178, 340, 655, 775, 1119, 1246
PspPI GGNCC 2 cut(s) 602, 1244
PspPPI RGGWCCY 1 cut(s) 1244
PstI CTGCAG 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 1 cut(s) 97
PvuI CGATCG 1 cut(s) 221
PvuII CAGCTG 2 cut(s) 626, 1259
RsaI GTAC 5 cut(s) 80, 244, 562, 798, 935
RsaNI GTAC 5 cut(s) 79, 243, 561, 797, 934
RseI CAYNNNNRTG 3 cut(s) 554, 648, 1076
SaqAI TTAA 2 cut(s) 1365, 1374
SatI GCNGC 8 cut(s) 342, 384, 624, 627, 711, 723, 1190, 1257
Sau3AI GATC 8 cut(s) 30, 97, 213, 218, 399, 539, 1107, 1326
Sau96I GGNCC 2 cut(s) 602, 1244
ScaI AGTACT 1 cut(s) 935
ScrFI CCNGG 2 cut(s) 1302, 1303
SduI GDGCHC 2 cut(s) 307, 776
SfaNI GCATC 3 cut(s) 811, 1047, 1232
SfcI CTRYAG 1 cut(s) 786
SfoI GGCGCC 1 cut(s) 1119
SinI GGWCC 1 cut(s) 1244
SmaI CCCGGG 1 cut(s) 1303
SmiMI CAYNNNNRTG 3 cut(s) 554, 648, 1076
SmlI CTYRAG 2 cut(s) 411, 1373
SmoI CTYRAG 2 cut(s) 411, 1373
SnaBI TACGTA 1 cut(s) 667
Sse9I AATT 9 cut(s) 107, 164, 439, 858, 885, 942, 1050, 1149, 1350
SsiI CCGC 5 cut(s) 342, 800, 974, 1089, 1254
SspDI GGCGCC 1 cut(s) 1117
SspI AATATT 1 cut(s) 1320
SspMI CTAG 4 cut(s) 371, 387, 395, 1220
StyD4I CCNGG 2 cut(s) 1300, 1301
StyI CCWWGG 1 cut(s) 1041
TaaI ACNGT 5 cut(s) 48, 229, 556, 997, 1075
TaiI ACGT 1 cut(s) 669
TaqI TCGA 2 cut(s) 56, 1200
TaqII GACCGA 1 cut(s) 299
TasI AATT 9 cut(s) 107, 164, 439, 858, 885, 942, 1050, 1149, 1350
TatI WGTACW 1 cut(s) 933
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 200, 1306
Tru1I TTAA 2 cut(s) 1365, 1374
Tru9I TTAA 2 cut(s) 1365, 1374
TscAI CASTG 2 cut(s) 514, 1080
TseFI GTSAC 1 cut(s) 594
TseI GCWGC 7 cut(s) 383, 623, 626, 710, 722, 1189, 1256
Tsp45I GTSAC 1 cut(s) 594
TspDTI ATGAA 4 cut(s) 282, 605, 1333, 1369
TspGWI ACGGA 2 cut(s) 189, 993
TspMI CCCGGG 1 cut(s) 1301
TspRI CASTG 2 cut(s) 514, 1080
Vha464I CTTAAG 1 cut(s) 1373
VpaK11BI GGWCC 1 cut(s) 1244
XagI CCTNNNNNAGG 1 cut(s) 130
XapI RAATTY 4 cut(s) 107, 439, 858, 942
XmaI CCCGGG 1 cut(s) 1301
XspI CTAG 4 cut(s) 371, 387, 395, 1220
ZrmI AGTACT 1 cut(s) 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.