Rw1G037450

Protein of unknown function (DUF819)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009936
Physical Location & Seq
Reverse (-)
65123107 .. 65125309
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G037450.1

Sequence Viewer

Length: 1365 bp
ATGTCCATGGTATCGAAGCTCTCCGTTTTATCCTGCTCAAGGCCGTCGTTGCCGGCAGAGTTGCAACGTTCAAGTTTCTCTTCCCGCCAAAACCTGAGTGTCGGTCTTTCACGCGCCACCGCCGGTGGCAGCTCATTTCCGTTAAAGAACACAAGGCGGCAGACCTTTCTCTCTCCTCCGGCTCCGCCTAAACCCGACCGATCCGTTACGGCGAAGTTCCAGCTCCAAACCCCTCTCATTTCTCCACATGATCAATGGGGCAACTGGACCGTACTCTTCGCCACCGGCACTTTCGGTATCTGGTCGGAGAAGAACACCAACATTGCGGGTGCATTGAGTGGCGCGTTGGTAAGCACATTGATTGGACTTGCGGCGAGCAATTTGGGAGTTATTTCGTCGAATGCACCAGCGTTATCGATTGTGTTGGAGTTTCTGCTCCCTCTGGCTGTGCCATTGCTGTTGTACAGGGCGGACTTGCGCCGTGTGATCAAATCAACCGGGCCTCTGCTCTTGGCCTTCTTGCTAGGATCAGTAGCGACGACAGTTGGAACAGTGGTGGCATATCTGTTGGTGCCGATGCGACCACTTGGGCCAGATAGTTGGAAAATAGCAGCTGCACTCATGGGAAGACACATCGGTGGATCTTTCAATTACGTTGCCATAGCCGATGCTCTTGGGGTTTCTCCGTCAATTTTAGCTGCTGGACTAGCCGCAGATAATGTCATTAATGCTGTATACTTTTCAACATTGTTTGCATTGGCTTCAAGAGTACCTGCAGAGGCTCCAATATCTGATACTGGCATTGCAATGGACGCAGAGTCCGACTCTGGAAACAAACTTCCTCTGGTACAAACTGCTACAGCCCTTGCCGTGTCCTTAGCCATCTGCAAGAGTGGCTATTATCTCACAAAATACTTTCGAATTCAAGGAGGTATCCTGCCAGCCGTAACAGCCATTGTGGTTGTTCTTGCGACTGTATTTCCAAAACAGTTTGCATATCTTGCACCATCTGGTGAGGCTATGGCCATGATCCTAATGCAGGTATTTTTCACGGTGGTGGGAGCGAGTGGGAGCATTTGGAGTGTGATCAACACTGCTCCTAGTATCTTCCTCTTTGCTTTAGTCCAGATTGCCGTCCATCTTGCTATGATTCTTGGATTAGGAAAGCTACTGGGATTTGACCTCAAGTTGTTGCTTATAGCATCAAATGCCAATATTGGAGGCCCAACAACAGCTTGTGGAATGGCCACAACCAAAGAATGGCATTCTATGATTGTCCCTGGTATTCTTGCAGGCATCTTCGGCATTTCCATTGCAACTTTCCTCGGCATTGGATTTGGGGTTGCCGTTCTAAAAGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF819

Protein Analysis

454

Amino Acids

47.28

Weight (kDa)

9.76

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF819 PF05684 90 - 452 8.8e-118 Protein of unknown function (DUF819)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 781, 1030
AccB1I GGYRCC 1 cut(s) 571
AccB7I CCANNNNNTGG 1 cut(s) 1260
AccI GTMKAC 1 cut(s) 735
AccII CGCG 2 cut(s) 114, 344
AciI CCGC 8 cut(s) 85, 120, 157, 185, 326, 371, 470, 711
AclI AACGTT 1 cut(s) 67
AclWI GGATC 4 cut(s) 195, 535, 649, 1024
AcoI YGGCCR 2 cut(s) 1023, 1245
AcsI RAATTY 1 cut(s) 921
AfaI GTAC 4 cut(s) 273, 464, 771, 849
AfiI CCNNNNNNNGG 3 cut(s) 39, 1039, 1260
AgsI TTSAA 5 cut(s) 72, 649, 744, 765, 926
AhdI GACNNNNNGTC 1 cut(s) 817
AjnI CCWGG 1 cut(s) 1279
AleI CACNNNNGTG 2 cut(s) 636, 1055
AluBI AGCT 7 cut(s) 19, 132, 223, 614, 698, 1168, 1235
AluI AGCT 7 cut(s) 19, 132, 223, 614, 698, 1168, 1235
AlwI GGATC 4 cut(s) 195, 535, 649, 1024
AoxI GGCC 7 cut(s) 41, 500, 513, 590, 1023, 1222, 1245
ApeKI GCWGC 4 cut(s) 129, 611, 614, 698
ApoI RAATTY 1 cut(s) 921
AseI ATTAAT 1 cut(s) 726
AspLEI GCGC 3 cut(s) 116, 344, 480
AspS9I GGNCC 4 cut(s) 267, 500, 590, 1223
AsuC2I CCSGG 1 cut(s) 499
AsuHPI GGTGA 1 cut(s) 1025
AsuII TTCGAA 1 cut(s) 919
AvaII GGWCC 1 cut(s) 267
BalI TGGCCA 2 cut(s) 1025, 1247
BanI GGYRCC 1 cut(s) 571
BbsI GAAGAC 1 cut(s) 634
BbvI GCAGC 4 cut(s) 141, 601, 623, 685
BccI CCATC 3 cut(s) 890, 1015, 1146
BceAI ACGGC 7 cut(s) 28, 225, 465, 854, 929, 1118, 1331
BciT130I CCWGG 1 cut(s) 1281
BciVI GTATCC 1 cut(s) 944
BclI TGATCA 3 cut(s) 250, 486, 1086
BcnI CCSGG 1 cut(s) 499
BfaI CTAG 3 cut(s) 524, 707, 1101
BfmI CTRYAG 2 cut(s) 774, 858
BfuAI ACCTGC 2 cut(s) 781, 1030
BfuI GTATCC 1 cut(s) 944
BisI GCNGC 7 cut(s) 130, 158, 372, 612, 615, 699, 711
BlsI GCNGC 7 cut(s) 131, 159, 373, 613, 616, 700, 712
Bme1390I CCNGG 2 cut(s) 499, 1281
Bme18I GGWCC 1 cut(s) 267
BmeRI GACNNNNNGTC 1 cut(s) 817
BmgT120I GGNCC 4 cut(s) 267, 500, 590, 1223
BmiI GGNNCC 3 cut(s) 183, 573, 783
BmrFI CCNGG 2 cut(s) 499, 1281
BmrI ACTGGG 1 cut(s) 1181
BmsI GCATC 4 cut(s) 567, 658, 1211, 1305
BmuI ACTGGG 1 cut(s) 1181
BpiI GAAGAC 1 cut(s) 634
BplI GAGNNNNNCTC 2 cut(s) 809, 841
Bpu10I CCTNAGC 1 cut(s) 877
Bpu14I TTCGAA 1 cut(s) 919
BpuEI CTTGAG 2 cut(s) 22, 1169
BpuMI CCSGG 1 cut(s) 499
Bsa29I ATCGAT 1 cut(s) 416
BsaJI CCNNGG 3 cut(s) 6, 1279, 1324
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 1039, 1260
Bse118I RCCGGY 3 cut(s) 52, 122, 284
Bse1I ACTGG 3 cut(s) 269, 802, 1176
Bse3DI GCAATG 5 cut(s) 321, 452, 801, 813, 1311
BseBI CCWGG 1 cut(s) 1281
BseCI ATCGAT 1 cut(s) 416
BseDI CCNNGG 3 cut(s) 6, 1279, 1324
BseLI CCNNNNNNNGG 3 cut(s) 39, 1039, 1260
BseMI GCAATG 5 cut(s) 321, 452, 801, 813, 1311
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 3 cut(s) 269, 802, 1176
BseRI GAGGAG 1 cut(s) 165
BseXI GCAGC 4 cut(s) 141, 601, 623, 685
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 2 cut(s) 114, 344
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 7 cut(s) 43, 502, 515, 592, 1025, 1224, 1247
BshNI GGYRCC 1 cut(s) 571
BshVI ATCGAT 1 cut(s) 416
BsiEI CGRYCG 1 cut(s) 199
BsiSI CCGG 5 cut(s) 53, 123, 179, 285, 498
BslFI GGGAC 1 cut(s) 1262
BslI CCNNNNNNNGG 3 cut(s) 39, 1039, 1260
BsmFI GGGAC 1 cut(s) 1262
BsmI GAATGC 2 cut(s) 406, 1264
BsnI GGCC 7 cut(s) 43, 502, 515, 592, 1025, 1224, 1247
Bsp119I TTCGAA 1 cut(s) 919
Bsp1407I TGTACA 1 cut(s) 462
Bsp143I GATC 7 cut(s) 200, 250, 486, 527, 641, 1029, 1086
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 8 cut(s) 85, 120, 157, 185, 326, 371, 470, 711
BspANI GGCC 7 cut(s) 43, 502, 515, 592, 1025, 1224, 1247
BspCNI CTCAG 1 cut(s) 87
BspDI ATCGAT 1 cut(s) 416
BspFNI CGCG 2 cut(s) 114, 344
BspLI GGNNCC 3 cut(s) 183, 573, 783
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 2 cut(s) 781, 1030
BspPI GGATC 4 cut(s) 195, 535, 649, 1024
BspT104I TTCGAA 1 cut(s) 919
BspT107I GGYRCC 1 cut(s) 571
BsrDI GCAATG 5 cut(s) 321, 452, 801, 813, 1311
BsrFI RCCGGY 3 cut(s) 52, 122, 284
BsrGI TGTACA 1 cut(s) 462
BsrI ACTGG 3 cut(s) 269, 802, 1176
BssAI RCCGGY 3 cut(s) 52, 122, 284
BssECI CCNNGG 3 cut(s) 6, 1279, 1324
BssMI GATC 7 cut(s) 200, 250, 486, 527, 641, 1029, 1086
BssNAI GTATAC 1 cut(s) 736
BssT1I CCWWGG 1 cut(s) 6
Bst1107I GTATAC 1 cut(s) 736
Bst2UI CCWGG 1 cut(s) 1281
Bst4CI ACNGT 6 cut(s) 271, 544, 553, 976, 990, 1054
Bst6I CTCTTC 2 cut(s) 85, 281
BstAPI GCANNNNNTGC 2 cut(s) 1001, 1208
BstAUI TGTACA 1 cut(s) 462
BstBI TTCGAA 1 cut(s) 919
BstC8I GCNNGC 4 cut(s) 54, 376, 942, 1294
BstDEI CTNAG 2 cut(s) 95, 877
BstDSI CCRYGG 1 cut(s) 6
BstENI CCTNNNNNAGG 2 cut(s) 37, 1037
BstFNI CGCG 2 cut(s) 114, 344
BstHHI GCGC 3 cut(s) 116, 344, 480
BstKTI GATC 7 cut(s) 203, 253, 489, 530, 644, 1032, 1089
BstMBI GATC 7 cut(s) 200, 250, 486, 527, 641, 1029, 1086
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 8 cut(s) 49, 707, 812, 894, 950, 1001, 1208, 1302
BstNI CCWGG 1 cut(s) 1281
BstSCI CCNGG 2 cut(s) 497, 1279
BstSFI CTRYAG 2 cut(s) 774, 858
BstUI CGCG 2 cut(s) 114, 344
BstV1I GCAGC 4 cut(s) 141, 601, 623, 685
BstV2I GAAGAC 1 cut(s) 634
BstX2I RGATCY 1 cut(s) 641
BstXI CCANNNNNNTGG 1 cut(s) 600
BstYI RGATCY 1 cut(s) 641
BstZ17I GTATAC 1 cut(s) 736
Bsu15I ATCGAT 1 cut(s) 416
BsuI GTATCC 1 cut(s) 944
BsuRI GGCC 7 cut(s) 43, 502, 515, 592, 1025, 1224, 1247
BsuTUI ATCGAT 1 cut(s) 416
BtgI CCRYGG 1 cut(s) 6
BtsI GCAGTG 1 cut(s) 1092
BtsIMutI CAGTG 2 cut(s) 558, 1092
BveI ACCTGC 2 cut(s) 781, 1030
Cac8I GCNNGC 4 cut(s) 54, 376, 942, 1294
CfoI GCGC 3 cut(s) 116, 344, 480
Cfr10I RCCGGY 3 cut(s) 52, 122, 284
Cfr13I GGNCC 4 cut(s) 267, 500, 590, 1223
ClaI ATCGAT 1 cut(s) 416
CseI GACGC 1 cut(s) 821
Csp6I GTAC 4 cut(s) 272, 463, 770, 848
CviAII CATG 5 cut(s) 7, 248, 622, 1027, 1360
CviQI GTAC 4 cut(s) 272, 463, 770, 848
DdeI CTNAG 2 cut(s) 95, 877
DpnI GATC 7 cut(s) 202, 252, 488, 529, 643, 1031, 1088
DpnII GATC 7 cut(s) 200, 250, 486, 527, 641, 1029, 1086
DriI GACNNNNNGTC 1 cut(s) 817
EaeI YGGCCR 2 cut(s) 1023, 1245
Eam1104I CTCTTC 2 cut(s) 85, 281
Eam1105I GACNNNNNGTC 1 cut(s) 817
EarI CTCTTC 2 cut(s) 85, 281
EciI GGCGGA 2 cut(s) 174, 485
Eco130I CCWWGG 1 cut(s) 6
Eco47I GGWCC 1 cut(s) 267
EcoNI CCTNNNNNAGG 2 cut(s) 37, 1037
EcoRI GAATTC 1 cut(s) 921
EcoRII CCWGG 1 cut(s) 1279
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 5 cut(s) 10, 251, 625, 1030, 1363
FalI AAGNNNNNCTT 2 cut(s) 64, 96
FaqI GGGAC 1 cut(s) 1262
FatI CATG 5 cut(s) 6, 247, 621, 1026, 1359
FauI CCCGC 2 cut(s) 92, 319
FbaI TGATCA 3 cut(s) 250, 486, 1086
FblI GTMKAC 1 cut(s) 735
Fnu4HI GCNGC 7 cut(s) 130, 158, 372, 612, 615, 699, 711
Fsp4HI GCNGC 7 cut(s) 130, 158, 372, 612, 615, 699, 711
FspBI CTAG 3 cut(s) 524, 707, 1101
GlaI GCGC 3 cut(s) 115, 343, 479
GluI GCNGC 7 cut(s) 130, 158, 372, 612, 615, 699, 711
HaeIII GGCC 7 cut(s) 43, 502, 515, 592, 1025, 1224, 1247
HapII CCGG 5 cut(s) 53, 123, 179, 285, 498
HgaI GACGC 1 cut(s) 821
HhaI GCGC 3 cut(s) 116, 344, 480
Hin1II CATG 5 cut(s) 10, 251, 625, 1030, 1363
Hin6I GCGC 3 cut(s) 114, 342, 478
HinP1I GCGC 3 cut(s) 114, 342, 478
HinfI GANTC 3 cut(s) 818, 824, 1150
HpaII CCGG 5 cut(s) 53, 123, 179, 285, 498
HphI GGTGA 1 cut(s) 1025
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 3 cut(s) 307, 793, 823
Hpy188III TCNNGA 3 cut(s) 765, 828, 1126
Hpy8I GTNNAC 1 cut(s) 736
Hpy99I CGWCG 3 cut(s) 49, 400, 541
HpyAV CCTTC 1 cut(s) 526
HpyCH4III ACNGT 6 cut(s) 271, 544, 553, 976, 990, 1054
HpyCH4IV ACGT 2 cut(s) 67, 654
HpyF10VI GCNNNNNNNGC 8 cut(s) 49, 707, 812, 894, 950, 1001, 1208, 1302
HpyF3I CTNAG 2 cut(s) 95, 877
HpySE526I ACGT 2 cut(s) 67, 654
Hsp92II CATG 5 cut(s) 10, 251, 625, 1030, 1363
HspAI GCGC 3 cut(s) 114, 342, 478
KroI GCCGGC 1 cut(s) 52
KroNI GCCGGC 1 cut(s) 54
Ksp22I TGATCA 3 cut(s) 250, 486, 1086
Kzo9I GATC 7 cut(s) 200, 250, 486, 527, 641, 1029, 1086
LmnI GCTCC 7 cut(s) 187, 228, 441, 787, 1061, 1071, 1102
Lsp1109I GCAGC 4 cut(s) 141, 601, 623, 685
LweI GCATC 4 cut(s) 567, 658, 1211, 1305
MaeI CTAG 3 cut(s) 524, 707, 1101
MaeII ACGT 2 cut(s) 67, 654
MaeIII GTNAC 2 cut(s) 205, 946
MalI GATC 7 cut(s) 202, 252, 488, 529, 643, 1031, 1088
MboI GATC 7 cut(s) 200, 250, 486, 527, 641, 1029, 1086
MboII GAAGA 6 cut(s) 72, 268, 322, 639, 1099, 1291
MflI RGATCY 1 cut(s) 641
MlsI TGGCCA 2 cut(s) 1025, 1247
MluCI AATT 4 cut(s) 379, 649, 690, 921
MluNI TGGCCA 2 cut(s) 1025, 1247
MlyI GAGTC 2 cut(s) 818, 827
MmeI TCCRAC 5 cut(s) 285, 405, 526, 581, 846
Mox20I TGGCCA 2 cut(s) 1025, 1247
MroNI GCCGGC 1 cut(s) 52
MscI TGGCCA 2 cut(s) 1025, 1247
MseI TTAA 2 cut(s) 143, 726
MslI CAYNNNNRTG 3 cut(s) 636, 806, 1055
Msp20I TGGCCA 2 cut(s) 1025, 1247
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 5 cut(s) 53, 123, 179, 285, 498
MspR9I CCNGG 2 cut(s) 499, 1281
Mva1269I GAATGC 2 cut(s) 406, 1264
MvaI CCWGG 1 cut(s) 1281
MvnI CGCG 2 cut(s) 114, 344
MwoI GCNNNNNNNGC 8 cut(s) 49, 707, 812, 894, 950, 1001, 1208, 1302
NaeI GCCGGC 1 cut(s) 54
NciI CCSGG 1 cut(s) 499
NcoI CCATGG 1 cut(s) 6
NdeII GATC 7 cut(s) 200, 250, 486, 527, 641, 1029, 1086
NgoMIV GCCGGC 1 cut(s) 52
NlaIII CATG 5 cut(s) 10, 251, 625, 1030, 1363
NlaIV GGNNCC 3 cut(s) 183, 573, 783
NmeAIII GCCGAG 1 cut(s) 1305
NspV TTCGAA 1 cut(s) 919
OliI CACNNNNGTG 2 cut(s) 636, 1055
PcsI WCGNNNNNNNCGW 1 cut(s) 819
PctI GAATGC 2 cut(s) 406, 1264
PdiI GCCGGC 1 cut(s) 54
PfeI GAWTC 1 cut(s) 1150
PflMI CCANNNNNTGG 1 cut(s) 1260
PkrI GCNGC 7 cut(s) 131, 159, 373, 613, 616, 700, 712
PleI GAGTC 2 cut(s) 818, 826
PpsI GAGTC 2 cut(s) 818, 826
PshBI ATTAAT 1 cut(s) 726
Psp1406I AACGTT 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 1279
PspGI CCWGG 1 cut(s) 1279
PspN4I GGNNCC 3 cut(s) 183, 573, 783
PspPI GGNCC 4 cut(s) 267, 500, 590, 1223
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 1 cut(s) 641
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 4 cut(s) 273, 464, 771, 849
RsaNI GTAC 4 cut(s) 272, 463, 770, 848
RseI CAYNNNNRTG 3 cut(s) 636, 806, 1055
SaqAI TTAA 2 cut(s) 143, 726
SatI GCNGC 7 cut(s) 130, 158, 372, 612, 615, 699, 711
Sau3AI GATC 7 cut(s) 200, 250, 486, 527, 641, 1029, 1086
Sau96I GGNCC 4 cut(s) 267, 500, 590, 1223
SchI GAGTC 2 cut(s) 818, 827
ScrFI CCNGG 2 cut(s) 499, 1281
SfaNI GCATC 4 cut(s) 567, 658, 1211, 1305
SfcI CTRYAG 2 cut(s) 774, 858
SfuI TTCGAA 1 cut(s) 919
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 1 cut(s) 267
SmiMI CAYNNNNRTG 3 cut(s) 636, 806, 1055
SmlI CTYRAG 2 cut(s) 37, 1184
SmoI CTYRAG 2 cut(s) 37, 1184
Sse9I AATT 4 cut(s) 379, 649, 690, 921
SsiI CCGC 8 cut(s) 85, 120, 157, 185, 326, 371, 470, 711
SspI AATATT 1 cut(s) 1216
SspMI CTAG 3 cut(s) 524, 707, 1101
StyD4I CCNGG 2 cut(s) 497, 1279
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 6 cut(s) 271, 544, 553, 976, 990, 1054
TaiI ACGT 2 cut(s) 70, 657
TaqI TCGA 4 cut(s) 14, 398, 416, 919
TaqII GACCGA 2 cut(s) 92, 213
TasI AATT 4 cut(s) 379, 649, 690, 921
TatI WGTACW 1 cut(s) 462
TauI GCSGC 3 cut(s) 160, 374, 713
TfiI GAWTC 1 cut(s) 1150
Tru1I TTAA 2 cut(s) 143, 726
Tru9I TTAA 2 cut(s) 143, 726
TscAI CASTG 2 cut(s) 558, 1099
TseI GCWGC 4 cut(s) 129, 611, 614, 698
TspGWI ACGGA 4 cut(s) 13, 129, 193, 675
TspRI CASTG 2 cut(s) 558, 1099
Van91I CCANNNNNTGG 1 cut(s) 1260
VpaK11BI GGWCC 1 cut(s) 267
VspI ATTAAT 1 cut(s) 726
XagI CCTNNNNNAGG 2 cut(s) 37, 1037
XapI RAATTY 1 cut(s) 921
XcmI CCANNNNNNNNNTGG 1 cut(s) 252
XmiI GTMKAC 1 cut(s) 735
XspI CTAG 3 cut(s) 524, 707, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.