FvH4_7g30151

Protein of unknown function (DUF819)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009936
Physical Location & Seq
Reverse (-)
22048921 .. 22050966
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30151.t1

Sequence Viewer

Length: 1377 bp
ATGGCCATGGCATCGAAGCTCACCGTCTTACCTGGATCCCATTCATCTTCGCCGGCAGGGTCGCAACGGTCAAGTTTCGCTTCCCGCCAAAACCTGAGTGTCCGTCTCTCACGCGCCACCGTCGGTGGCAGCTCAATCCAGTTAATCAACACAAGGCAACAGCAGCAGAGCTTTCTCTCTCCTCCGGCTCCGGCTCCGGCTAACTCCGTCCGATCTGTCACCGCCAGATTCCAGCTCCAAGCGCCGCTCATTTCTCCTCATGACCAATGGGGCAACTGGACCGTCCTCTTCGCCACCGGCGCTTTCGGTATCTGGTCGGAGAAGAACACCAACATTGGCGGTGCATTGAGCGGCGCGTTGGTGAGCACATTGATTGGACTTGCTGCTAGCAATTTGGGAGTGATTTCGTCGAATGCGCCGGCGTTTTCGATTGTGTTGGAGTTTCTGCTGCCTCTGGCTGTGCCATTGCTGCTGTACAGGGCGGATATGCGCCGTGTGATCAAATCAACCGGGGCGCTGCTCTTGGCCTTCTTGCTAGGATCAGTTGCGACGACAGTTGGAACGGTAGTGGCATATCTATTGGTGCCTATGCGAGCGCTTGGTCCAGATAGTTGGAAAATAGCAGCTGCACTCATGGGTAGACACATTGGTGGAGCTGTCAATTACGTTGCCATAGCCGATGCTCTTGGGGTCACTCCGTCAATTTTAGCTGCTGGACTAGCAGCAGATAATGTCATTTGTGCTGTATATTTCTCGACATTGTTTGCATTGGCTTCAAGAGTACCTGCAGAAGCTCCAATATCTGATACTGGCACTGCAAACGATGCAGAATCTGACAATGGAAACAAGCTTCCTCTGGTACAAACTGCTACGGCCCTTGCTGTGTCCTTAGCCATCTGCAAGAGTGGCTATTACCTCACAAAATACTTTCGAATTCAAGGAGGTATCCTTCCAGCCGTAACAGCCATTGTGGTTGTTCTTGCGACTGTATTTCCGAAACAGTTTGCATATCTTGCACCATCTGGTGAAGCTATGGCCATGATCCTAATGCAGGTATTTTTCACTGTGGTGGGAGCAAGTGGAAACATATGGAGTGTGATCAACACTGCTCCTAGTATCTTCCTCTTTGCTTTAGTCCAGATTGCTGTCCACCTTGCTATGATACTTGGATTGGGAAAGCTACTAGGATTCAACCTCAAGTTGCTGCTTATAGCATCAAATGCCAACGTCGGAGGCCCTACAACAGCTTGTGGAATGGCCACAGCCAAAGAATGGCATTCTATGATTGTTCCTGGAATTCTTGCAGGCATCTTTGGCATTGCCATCGCAACTTTCCTCGGCATTGGATTTGGGGTCGCCGTTCTAAAAGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF819

Protein Analysis

459

Amino Acids

47.32

Weight (kDa)

9.85

Isoelectric Point (pI)

34.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF819 PF05684 94 - 456 2.4e-120 Protein of unknown function (DUF819)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 793, 1042
AccB1I GGYRCC 1 cut(s) 583
AccB7I CCANNNNNTGG 1 cut(s) 1272
AccBSI CCGCTC 2 cut(s) 247, 351
AccI GTMKAC 1 cut(s) 640
AccII CGCG 2 cut(s) 114, 356
AciI CCGC 6 cut(s) 85, 222, 245, 339, 351, 482
AclWI GGATC 4 cut(s) 30, 43, 547, 1036
AcoI YGGCCR 3 cut(s) 3, 1035, 1257
AcsI RAATTY 2 cut(s) 933, 1296
AfaI GTAC 3 cut(s) 476, 783, 861
AfeI AGCGCT 1 cut(s) 597
AfiI CCNNNNNNNGG 2 cut(s) 1051, 1272
AgsI TTSAA 3 cut(s) 777, 938, 1192
AjnI CCWGG 2 cut(s) 31, 1291
AleI CACNNNNGTG 2 cut(s) 648, 1067
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 4 cut(s) 30, 43, 547, 1036
AlwNI CAGNNNCTG 1 cut(s) 833
Aor51HI AGCGCT 1 cut(s) 597
AoxI GGCC 6 cut(s) 3, 525, 873, 1035, 1234, 1257
ApoI RAATTY 2 cut(s) 933, 1296
AspLEI GCGC 8 cut(s) 116, 244, 302, 356, 418, 492, 517, 598
AspS9I GGNCC 4 cut(s) 279, 602, 874, 1235
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 4 cut(s) 13, 211, 373, 1037
AsuII TTCGAA 1 cut(s) 931
AsuNHI GCTAGC 1 cut(s) 386
AvaII GGWCC 2 cut(s) 279, 602
BalI TGGCCA 3 cut(s) 5, 1037, 1259
BamHI GGATCC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 583
Bbv12I GWGCWC 1 cut(s) 368
BccI CCATC 3 cut(s) 902, 1027, 1331
BceAI ACGGC 4 cut(s) 477, 888, 941, 1343
BcgI CGANNNNNNTGC 2 cut(s) 1306, 1340
BciT130I CCWGG 2 cut(s) 33, 1293
BciVI GTATCC 1 cut(s) 956
BclI TGATCA 2 cut(s) 498, 1098
BcnI CCSGG 1 cut(s) 511
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 5 cut(s) 387, 536, 719, 1113, 1184
BfmI CTRYAG 1 cut(s) 786
BfoI RGCGCY 4 cut(s) 245, 303, 518, 599
BfuAI ACCTGC 2 cut(s) 793, 1042
BfuI GTATCC 1 cut(s) 956
Bme1390I CCNGG 3 cut(s) 33, 511, 1293
Bme18I GGWCC 2 cut(s) 279, 602
BmgT120I GGNCC 4 cut(s) 279, 602, 874, 1235
BmiI GGNNCC 4 cut(s) 37, 189, 195, 585
BmrFI CCNGG 3 cut(s) 33, 511, 1293
BmsI GCATC 5 cut(s) 20, 670, 814, 1223, 1317
BmtI GCTAGC 1 cut(s) 390
Bpu10I CCTNAGC 1 cut(s) 889
Bpu14I TTCGAA 1 cut(s) 931
BpuEI CTTGAG 1 cut(s) 1181
BpuMI CCSGG 1 cut(s) 511
BsaJI CCNNGG 3 cut(s) 6, 510, 1336
Bsc4I CCNNNNNNNGG 2 cut(s) 1051, 1272
Bse118I RCCGGY 3 cut(s) 52, 296, 418
Bse1I ACTGG 3 cut(s) 139, 281, 814
Bse3DI GCAATG 2 cut(s) 464, 1317
BseBI CCWGG 2 cut(s) 33, 1293
BseDI CCNNGG 3 cut(s) 6, 510, 1336
BseLI CCNNNNNNNGG 2 cut(s) 1051, 1272
BseMI GCAATG 2 cut(s) 464, 1317
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 3 cut(s) 139, 281, 814
BseRI GAGGAG 2 cut(s) 171, 246
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 2 cut(s) 114, 356
BshFI GGCC 6 cut(s) 5, 527, 875, 1037, 1236, 1259
BshNI GGYRCC 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 7 cut(s) 53, 185, 191, 197, 297, 419, 510
BslI CCNNNNNNNGG 2 cut(s) 1051, 1272
BsmAI GTCTC 1 cut(s) 110
BsmBI CGTCTC 1 cut(s) 110
BsmI GAATGC 2 cut(s) 418, 1276
BsnI GGCC 6 cut(s) 5, 527, 875, 1037, 1236, 1259
Bsp119I TTCGAA 1 cut(s) 931
Bsp1286I GDGCHC 1 cut(s) 368
Bsp1407I TGTACA 1 cut(s) 474
Bsp143I GATC 6 cut(s) 35, 212, 498, 539, 1041, 1098
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 6 cut(s) 85, 222, 245, 339, 351, 482
BspANI GGCC 6 cut(s) 5, 527, 875, 1037, 1236, 1259
BspCNI CTCAG 1 cut(s) 87
BspFNI CGCG 2 cut(s) 114, 356
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 4 cut(s) 37, 189, 195, 585
BspMAI CTGCAG 1 cut(s) 790
BspMI ACCTGC 2 cut(s) 793, 1042
BspOI GCTAGC 1 cut(s) 390
BspPI GGATC 4 cut(s) 30, 43, 547, 1036
BspT104I TTCGAA 1 cut(s) 931
BspT107I GGYRCC 1 cut(s) 583
BsrBI CCGCTC 2 cut(s) 247, 351
BsrDI GCAATG 2 cut(s) 464, 1317
BsrFI RCCGGY 3 cut(s) 52, 296, 418
BsrGI TGTACA 1 cut(s) 474
BsrI ACTGG 3 cut(s) 139, 281, 814
BssAI RCCGGY 3 cut(s) 52, 296, 418
BssECI CCNNGG 3 cut(s) 6, 510, 1336
BssMI GATC 6 cut(s) 35, 212, 498, 539, 1041, 1098
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 33, 1293
Bst4CI ACNGT 9 cut(s) 25, 69, 121, 283, 556, 565, 988, 1002, 1066
Bst6I CTCTTC 1 cut(s) 293
BstAPI GCANNNNNTGC 3 cut(s) 824, 1013, 1220
BstAUI TGTACA 1 cut(s) 474
BstBI TTCGAA 1 cut(s) 931
BstC8I GCNNGC 5 cut(s) 54, 388, 420, 594, 1306
BstDEI CTNAG 2 cut(s) 95, 889
BstDSI CCRYGG 1 cut(s) 6
BstENI CCTNNNNNAGG 1 cut(s) 1049
BstFNI CGCG 2 cut(s) 114, 356
BstH2I RGCGCY 4 cut(s) 245, 303, 518, 599
BstHHI GCGC 8 cut(s) 116, 244, 302, 356, 418, 492, 517, 598
BstKTI GATC 6 cut(s) 38, 215, 501, 542, 1044, 1101
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 6 cut(s) 35, 212, 498, 539, 1041, 1098
BstNI CCWGG 2 cut(s) 33, 1293
BstSCI CCNGG 3 cut(s) 31, 509, 1291
BstSFI CTRYAG 1 cut(s) 786
BstUI CGCG 2 cut(s) 114, 356
BstX2I RGATCY 1 cut(s) 35
BstXI CCANNNNNNTGG 1 cut(s) 612
BstYI RGATCY 1 cut(s) 35
BsuI GTATCC 1 cut(s) 956
BsuRI GGCC 6 cut(s) 5, 527, 875, 1037, 1236, 1259
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 1309
BtsI GCAGTG 2 cut(s) 813, 1104
BtsIMutI CAGTG 3 cut(s) 813, 1062, 1104
BveI ACCTGC 2 cut(s) 793, 1042
Cac8I GCNNGC 5 cut(s) 54, 388, 420, 594, 1306
CaiI CAGNNNCTG 1 cut(s) 833
CciI TCATGA 1 cut(s) 259
CfoI GCGC 8 cut(s) 116, 244, 302, 356, 418, 492, 517, 598
Cfr10I RCCGGY 3 cut(s) 52, 296, 418
Cfr13I GGNCC 4 cut(s) 279, 602, 874, 1235
Csp6I GTAC 3 cut(s) 475, 782, 860
CviAII CATG 5 cut(s) 7, 260, 634, 1039, 1372
CviQI GTAC 3 cut(s) 475, 782, 860
DdeI CTNAG 2 cut(s) 95, 889
DpnI GATC 6 cut(s) 37, 214, 500, 541, 1043, 1100
DpnII GATC 6 cut(s) 35, 212, 498, 539, 1041, 1098
EaeI YGGCCR 3 cut(s) 3, 1035, 1257
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 6
Eco47I GGWCC 2 cut(s) 279, 602
Eco47III AGCGCT 1 cut(s) 597
EcoNI CCTNNNNNAGG 1 cut(s) 1049
EcoO109I RGGNCCY 1 cut(s) 1235
EcoRI GAATTC 2 cut(s) 933, 1296
EcoRII CCWGG 2 cut(s) 31, 1291
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 5 cut(s) 10, 263, 637, 1042, 1375
FalI AAGNNNNNCTT 2 cut(s) 64, 96
FatI CATG 5 cut(s) 6, 259, 633, 1038, 1371
FauI CCCGC 1 cut(s) 92
FauNDI CATATG 1 cut(s) 1088
FbaI TGATCA 2 cut(s) 498, 1098
FblI GTMKAC 1 cut(s) 640
FspBI CTAG 5 cut(s) 387, 536, 719, 1113, 1184
GlaI GCGC 8 cut(s) 115, 243, 301, 355, 417, 491, 516, 597
HaeII RGCGCY 4 cut(s) 245, 303, 518, 599
HaeIII GGCC 6 cut(s) 5, 527, 875, 1037, 1236, 1259
HapII CCGG 7 cut(s) 53, 185, 191, 197, 297, 419, 510
HhaI GCGC 8 cut(s) 116, 244, 302, 356, 418, 492, 517, 598
Hin1II CATG 5 cut(s) 10, 263, 637, 1042, 1375
Hin6I GCGC 8 cut(s) 114, 242, 300, 354, 416, 490, 515, 596
HinP1I GCGC 8 cut(s) 114, 242, 300, 354, 416, 490, 515, 596
HindIII AAGCTT 1 cut(s) 848
HinfI GANTC 3 cut(s) 228, 830, 1188
HpaII CCGG 7 cut(s) 53, 185, 191, 197, 297, 419, 510
HphI GGTGA 4 cut(s) 13, 211, 373, 1037
Hpy166II GTNNAC 2 cut(s) 641, 1150
Hpy188I TCNGA 6 cut(s) 212, 319, 805, 835, 996, 1232
Hpy188III TCNNGA 5 cut(s) 260, 605, 754, 777, 1138
Hpy8I GTNNAC 2 cut(s) 641, 1150
Hpy99I CGWCG 4 cut(s) 125, 412, 553, 1232
HpyAV CCTTC 2 cut(s) 538, 959
HpyCH4III ACNGT 9 cut(s) 25, 69, 121, 283, 556, 565, 988, 1002, 1066
HpyCH4IV ACGT 2 cut(s) 666, 1227
HpyF3I CTNAG 2 cut(s) 95, 889
HpySE526I ACGT 2 cut(s) 666, 1227
Hsp92II CATG 5 cut(s) 10, 263, 637, 1042, 1375
HspAI GCGC 8 cut(s) 114, 242, 300, 354, 416, 490, 515, 596
KroI GCCGGC 2 cut(s) 52, 418
KroNI GCCGGC 2 cut(s) 54, 420
Ksp22I TGATCA 2 cut(s) 498, 1098
Kzo9I GATC 6 cut(s) 35, 212, 498, 539, 1041, 1098
LmnI GCTCC 7 cut(s) 193, 199, 240, 653, 799, 1073, 1114
LweI GCATC 5 cut(s) 20, 670, 814, 1223, 1317
MaeI CTAG 5 cut(s) 387, 536, 719, 1113, 1184
MaeII ACGT 2 cut(s) 666, 1227
MaeIII GTNAC 3 cut(s) 217, 691, 958
MalI GATC 6 cut(s) 37, 214, 500, 541, 1043, 1100
MbiI CCGCTC 2 cut(s) 247, 351
MboI GATC 6 cut(s) 35, 212, 498, 539, 1041, 1098
MboII GAAGA 4 cut(s) 39, 280, 334, 1111
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 368
MlsI TGGCCA 3 cut(s) 5, 1037, 1259
MluCI AATT 5 cut(s) 391, 661, 702, 933, 1296
MluNI TGGCCA 3 cut(s) 5, 1037, 1259
MmeI TCCRAC 5 cut(s) 297, 417, 538, 593, 1210
Mox20I TGGCCA 3 cut(s) 5, 1037, 1259
MreI CGCCGGCG 1 cut(s) 418
MroNI GCCGGC 2 cut(s) 52, 418
MscI TGGCCA 3 cut(s) 5, 1037, 1259
MseI TTAA 1 cut(s) 143
MslI CAYNNNNRTG 2 cut(s) 648, 1067
Msp20I TGGCCA 3 cut(s) 5, 1037, 1259
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 7 cut(s) 53, 185, 191, 197, 297, 419, 510
MspR9I CCNGG 3 cut(s) 33, 511, 1293
Mva1269I GAATGC 2 cut(s) 418, 1276
MvaI CCWGG 2 cut(s) 33, 1293
MvnI CGCG 2 cut(s) 114, 356
NaeI GCCGGC 2 cut(s) 54, 420
NciI CCSGG 1 cut(s) 511
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 1088
NdeII GATC 6 cut(s) 35, 212, 498, 539, 1041, 1098
NgoMIV GCCGGC 2 cut(s) 52, 418
NheI GCTAGC 1 cut(s) 386
NlaIII CATG 5 cut(s) 10, 263, 637, 1042, 1375
NlaIV GGNNCC 4 cut(s) 37, 189, 195, 585
NmeAIII GCCGAG 1 cut(s) 1317
NmuCI GTSAC 2 cut(s) 217, 691
NspV TTCGAA 1 cut(s) 931
OliI CACNNNNGTG 2 cut(s) 648, 1067
PagI TCATGA 1 cut(s) 259
PctI GAATGC 2 cut(s) 418, 1276
PdiI GCCGGC 2 cut(s) 54, 420
PfeI GAWTC 3 cut(s) 228, 830, 1188
PflMI CCANNNNNTGG 1 cut(s) 1272
PfoI TCCNGGA 1 cut(s) 1291
Psp6I CCWGG 2 cut(s) 31, 1291
PspGI CCWGG 2 cut(s) 31, 1291
PspN4I GGNNCC 4 cut(s) 37, 189, 195, 585
PspPI GGNCC 4 cut(s) 279, 602, 874, 1235
PstI CTGCAG 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 3 cut(s) 476, 783, 861
RsaNI GTAC 3 cut(s) 475, 782, 860
RseI CAYNNNNRTG 2 cut(s) 648, 1067
SaqAI TTAA 1 cut(s) 143
Sau3AI GATC 6 cut(s) 35, 212, 498, 539, 1041, 1098
Sau96I GGNCC 4 cut(s) 279, 602, 874, 1235
ScrFI CCNGG 3 cut(s) 33, 511, 1293
SduI GDGCHC 1 cut(s) 368
SfaNI GCATC 5 cut(s) 20, 670, 814, 1223, 1317
SfcI CTRYAG 1 cut(s) 786
SfuI TTCGAA 1 cut(s) 931
SgrAI CRCCGGYG 2 cut(s) 296, 418
SinI GGWCC 2 cut(s) 279, 602
SmiMI CAYNNNNRTG 2 cut(s) 648, 1067
SmlI CTYRAG 1 cut(s) 1196
SmoI CTYRAG 1 cut(s) 1196
Sse9I AATT 5 cut(s) 391, 661, 702, 933, 1296
SsiI CCGC 6 cut(s) 85, 222, 245, 339, 351, 482
SspMI CTAG 5 cut(s) 387, 536, 719, 1113, 1184
StyD4I CCNGG 3 cut(s) 31, 509, 1291
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 9 cut(s) 25, 69, 121, 283, 556, 565, 988, 1002, 1066
TaiI ACGT 2 cut(s) 669, 1230
TaqI TCGA 5 cut(s) 14, 410, 428, 755, 931
TasI AATT 5 cut(s) 391, 661, 702, 933, 1296
TatI WGTACW 1 cut(s) 474
TauI GCSGC 2 cut(s) 247, 354
TfiI GAWTC 3 cut(s) 228, 830, 1188
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 3 cut(s) 820, 1069, 1111
TseFI GTSAC 2 cut(s) 217, 691
Tsp45I GTSAC 2 cut(s) 217, 691
TspDTI ATGAA 1 cut(s) 33
TspGWI ACGGA 3 cut(s) 92, 196, 687
TspRI CASTG 3 cut(s) 820, 1069, 1111
Van91I CCANNNNNTGG 1 cut(s) 1272
VpaK11BI GGWCC 2 cut(s) 279, 602
XagI CCTNNNNNAGG 1 cut(s) 1049
XapI RAATTY 2 cut(s) 933, 1296
XmiI GTMKAC 1 cut(s) 640
XspI CTAG 5 cut(s) 387, 536, 719, 1113, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.