Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0013903

Physical Location & Seq

Genomic Coordinates Forward (+)

21643767 .. 21647774

Gene Structure 4008 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G53350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGGCCGCAGCTTTAAGGTCGAATACGTCAAGAGAAACCGCTTCTCTTACACTTTCTCATTTTAGATACTTTATCTTCAACCGTATCCACACCGCTCGAACCGCCACAAGTCCTCCTCACTGTAATCACCGTTCTAAATCCGATGAGAAATTCCCCTATAAAATTTCCTCTCTTGGAACGAGTTTCTTGGATAATCGAGGAGGAGGAGAGAGACGTAATTCGACTAAGTGTTATGCTGCTCAGAAGAAGCTCAGCGGTGTTGGTTCTTCTGTTGTTATTCTGAGCTCACAAGGAGACCCACCGGATCTATGGCAGCCTCCTGGTGATGGAGTTTCCGTTAGGGTTAATGGGTCCAGTGTTAATCTCGGTCGTGGTGGTGGTGGTGGTGGAAGTAGTCCTGGTGGTCCTGGTAACGGAACAGGGTCGAATTCGAAAGAAGATTGTTGGGGTGGATCCAATCTTGGGTCTGATTTTCCGACACCAAAGGAGATATGTAAGGGTTTGAACAAGTTCGTGATTGGTCAGGAAAGAGCCAAAAAGGTACTTTCAGTGGCAGTTTACAATCACTACAAGAGGATATATCACGAATCATCACAGAAACGGTCTGCGGGAGAAACAGATAGCACTGCAGCGAAACCAGCAGATGATGATATGGTAGAACTAGAGAAGAGTAACATTCTCCTGATGGGACCAACTGGGTCAGGGAAGACACTACTTGCGAAAACCTTGGCACGATTTGTAAATGTTCCTTTTGTCATTGCGGATGCAACCACCCTGACTCAGGCTGGTTATGTGGGAGAGGACGTCGAGTCTATATTATACAAACTTCTCACAGTTGCAGATTATAATGTTGCAGCTGCACAGCAAGGGATTGTTTACATTGATGAAGTCGACAAGATAACAAAGAAGGCAGAAAGCCTTAACATCAGCAGAGATGTATCAGGAGAAGGTGTTCAACAAGCATTGCTAAAAATGCTGGAAGGAACAATTGTAAATGTACCTGAGAAAGGCGCTAGGAAACACCCTAGAGGTGACAATATCCAGATAGACACAAAGGACATACTCTTCATATGTGGTGGTGCTTTTGTAGACATTGAAAAGACTATCTCAGAGAGGCGCCATGATTCTTCAATAGGGTTTGGTGCTCCTGTACGTGCCAACATGAGGGCTGGTGGTGTTACAAACGCCGCTGTTGCATCAAACCTAATGGAAACTGTGGAAAGCAGTGACCTGATTGCTTATGGTTTGATTCCTGAATTTGTTGGACGATTTCCAGTTCTCGTCAGTTTGTCTGCTTTGACTGAGAACCAGCTTATGCAGGTGTTGACAGAACCCAAGAACGCTCTTGGAAAGCAATACAAAAAGATGTACCAGATGAATAGTGTAAAGCTGCATTTCACAGAAAGCGCACTGCGGCTTATAGCTAGAAAAGCAATAACGAAAAACACGGGTGCTCGTGGCTTAAGAGCTCTTCTAGAAAGCATCCTCATGGACTCTATGTACGAGATTCCGGACGAGGGTACAGGCAGCGATATGATAGAAGCGGTTGTGGTGGATGAAGAAGCAGTTGAAGGAGAAGGGCGTCGAGGCTCTGGAGCTAAGATTCTCCGTGGTAAAGGAGCATTGGCTCGGTATCTCTCTGAAACCAATTCCAAAGATTCCCCACAGACGACAAAGGAGGGATCGGATGGAGAAACTGAAGTGGAGGCAGAGATTCCATCTGTTGTAGCAAGTATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019538 GO:0030163 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042623 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0070011 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04112 map04112

Pfam Domains

Protein Families

AAA_2 ClpB_D2-small

Protein Analysis

579

Amino Acids

61.96

Weight (kDa)

7.62

Isoelectric Point (pI)

39.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_2	PF07724	222 - 426	3.1e-30	AAA domain (Cdc48 subfamily)
AAA	PF00004	226 - 332	2.8e-14	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	433 - 507	6.1e-10	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0013903)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53350
fragaria_vesca	FvH4_7g31580
malus_domestica	MD01G1218600.v1.1 MD07G1288500.v1.1
prunus_persica	Prupe.2G310000_v2.0.a1
pyrus_communis	pycom01g22850 pycom07g26110
rosa_chinensis	RchiOBHm_Chr1g0380581
rosa_laevigata	RLG00000026283
rosa_multiflora	Rmu_ssc0000015.1_g000011
rosa_roxburghii	Rroxscaffold_4G00279170
rosa_rugosa	Rorug01G0422700
rosa_samantha	Rh1AG447200 Rh1BG402500 Rh1CG417000 Rh1DG432700
rosa_wichuraiana	Rw1G038900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	846
AarI	CACCTGC	1 cut(s)	1309
AatII	GACGTC	1 cut(s)	807
Acc36I	ACCTGC	1 cut(s)	1309
AccB1I	GGYRCC	1 cut(s)	1116
AccBSI	CCGCTC	1 cut(s)	95
AccI	GTMKAC	2 cut(s)	891, 1089
AccIII	TCCGGA	1 cut(s)	1510
AclWI	GGATC	4 cut(s)	312, 447, 460, 1690
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	4 cut(s)	149, 162, 427, 1256
AcuI	CTGAAG	1 cut(s)	1719
AcyI	GRCGYC	3 cut(s)	804, 1117, 1582
AfaI	GTAC	6 cut(s)	543, 999, 1152, 1370, 1502, 1522
AfiI	CCNNNNNNNGG	7 cut(s)	326, 413, 462, 781, 1007, 1164, 1517
AflII	CTTAAG	1 cut(s)	1462
AgsI	TTSAA	6 cut(s)	79, 505, 956, 1097, 1131, 1571
AhdI	GACNNNNNGTC	1 cut(s)	808
AjnI	CCWGG	3 cut(s)	319, 397, 406
AloI	GAACNNNNNNTCC	2 cut(s)	936, 968
AluBI	AGCT	9 cut(s)	11, 250, 285, 857, 1312, 1390, 1424, 1469, 1598
AluI	AGCT	9 cut(s)	11, 250, 285, 857, 1312, 1390, 1424, 1469, 1598
Alw21I	GWGCWC	4 cut(s)	287, 1147, 1456, 1471
Alw26I	GTCTC	2 cut(s)	205, 288
AlwI	GGATC	4 cut(s)	312, 447, 460, 1690
Aor13HI	TCCGGA	1 cut(s)	1510
AoxI	GGCC	1 cut(s)	3
ApeKI	GCWGC	8 cut(s)	8, 236, 313, 629, 854, 857, 1390, 1527
ApoI	RAATTY	4 cut(s)	149, 162, 427, 1256
Asp700I	GAANNNNTTC	2 cut(s)	509, 951
AspLEI	GCGC	3 cut(s)	1013, 1119, 1409
AspS9I	GGNCC	3 cut(s)	351, 404, 689
AsuHPI	GGTGA	3 cut(s)	119, 335, 1043
AsuII	TTCGAA	1 cut(s)	431
AvaII	GGWCC	3 cut(s)	351, 404, 689
BamHI	GGATCC	1 cut(s)	452
BanI	GGYRCC	1 cut(s)	1116
BanII	GRGCYC	2 cut(s)	287, 1471
BauI	CACGAG	1 cut(s)	1455
BbsI	GAAGAC	1 cut(s)	713
Bbv12I	GWGCWC	4 cut(s)	287, 1147, 1456, 1471
BbvI	GCAGC	8 cut(s)	20, 223, 325, 641, 844, 866, 1377, 1539
BccI	CCATC	4 cut(s)	320, 679, 1682, 1726
BciT130I	CCWGG	3 cut(s)	321, 399, 408
BciVI	GTATCC	1 cut(s)	95
BcoDI	GTCTC	2 cut(s)	205, 288
BfaI	CTAG	5 cut(s)	662, 1014, 1026, 1425, 1475
BfmI	CTRYAG	1 cut(s)	627
BfoI	RGCGCY	2 cut(s)	1014, 1120
BfrI	CTTAAG	1 cut(s)	1462
BfuAI	ACCTGC	1 cut(s)	1309
BfuI	GTATCC	1 cut(s)	95
BlpI	GCTNAGC	1 cut(s)	251
Bme1390I	CCNGG	3 cut(s)	321, 399, 408
Bme18I	GGWCC	3 cut(s)	351, 404, 689
BmeRI	GACNNNNNGTC	1 cut(s)	808
BmgT120I	GGNCC	3 cut(s)	351, 404, 689
BmiI	GGNNCC	4 cut(s)	352, 454, 690, 1118
BmrFI	CCNGG	3 cut(s)	321, 399, 408
BmrI	ACTGGG	1 cut(s)	705
BmsI	GCATC	3 cut(s)	754, 1205, 1491
BmuI	ACTGGG	1 cut(s)	705
BpiI	GAAGAC	1 cut(s)	713
BpmI	CTGGAG	1 cut(s)	1614
Bpu1102I	GCTNAGC	1 cut(s)	251
Bpu14I	TTCGAA	1 cut(s)	431
BsaAI	YACGTR	1 cut(s)	1154
BsaHI	GRCGYC	3 cut(s)	804, 1117, 1582
BsaI	GGTCTC	1 cut(s)	288
BsaJI	CCNNGG	2 cut(s)	726, 1609
BsaWI	WCCGGW	2 cut(s)	301, 1510
BsaXI	ACNNNNNCTCC	8 cut(s)	97, 127, 321, 351, 789, 819, 936, 966
Bsc4I	CCNNNNNNNGG	7 cut(s)	326, 413, 462, 781, 1007, 1164, 1517
Bse1I	ACTGG	3 cut(s)	354, 700, 1274
Bse3DI	GCAATG	2 cut(s)	756, 962
BseAI	TCCGGA	1 cut(s)	1510
BseBI	CCWGG	3 cut(s)	321, 399, 408
BseDI	CCNNGG	2 cut(s)	726, 1609
BseGI	GGATG	4 cut(s)	769, 1482, 1561, 1693
BseLI	CCNNNNNNNGG	7 cut(s)	326, 413, 462, 781, 1007, 1164, 1517
BseMI	GCAATG	2 cut(s)	756, 962
BseMII	CTCAG	7 cut(s)	254, 265, 272, 794, 993, 1122, 1293
BseNI	ACTGG	3 cut(s)	354, 700, 1274
BseRI	GAGGAG	4 cut(s)	105, 213, 216, 219
BseXI	GCAGC	8 cut(s)	20, 223, 325, 641, 844, 866, 1377, 1539
BsgI	GTGCAG	1 cut(s)	843
Bsh1285I	CGRYCG	1 cut(s)	370
BshFI	GGCC	1 cut(s)	5
BshNI	GGYRCC	1 cut(s)	1116
BsiEI	CGRYCG	1 cut(s)	370
BsiHKAI	GWGCWC	4 cut(s)	287, 1147, 1456, 1471
BsiSI	CCGG	2 cut(s)	302, 1511
BslFI	GGGAC	1 cut(s)	702
BslI	CCNNNNNNNGG	7 cut(s)	326, 413, 462, 781, 1007, 1164, 1517
BsmAI	GTCTC	2 cut(s)	205, 288
BsmBI	CGTCTC	1 cut(s)	205
BsmFI	GGGAC	1 cut(s)	702
BsnI	GGCC	1 cut(s)	5
Bso31I	GGTCTC	1 cut(s)	288
Bsp119I	TTCGAA	1 cut(s)	431
Bsp1286I	GDGCHC	4 cut(s)	287, 1147, 1456, 1471
Bsp13I	TCCGGA	1 cut(s)	1510
Bsp143I	GATC	3 cut(s)	304, 452, 1682
Bsp1720I	GCTNAGC	1 cut(s)	251
BspANI	GGCC	1 cut(s)	5
BspCNI	CTCAG	7 cut(s)	253, 264, 273, 793, 994, 1121, 1294
BspEI	TCCGGA	1 cut(s)	1510
BspLI	GGNNCC	4 cut(s)	352, 454, 690, 1118
BspMAI	CTGCAG	1 cut(s)	631
BspMI	ACCTGC	1 cut(s)	1309
BspPI	GGATC	4 cut(s)	312, 447, 460, 1690
BspQI	GCTCTTC	1 cut(s)	1476
BspT104I	TTCGAA	1 cut(s)	431
BspT107I	GGYRCC	1 cut(s)	1116
BspTI	CTTAAG	1 cut(s)	1462
BspTNI	GGTCTC	1 cut(s)	288
BsrBI	CCGCTC	1 cut(s)	95
BsrDI	GCAATG	2 cut(s)	756, 962
BsrI	ACTGG	3 cut(s)	354, 700, 1274
BssECI	CCNNGG	2 cut(s)	726, 1609
BssMI	GATC	3 cut(s)	304, 452, 1682
BssNI	GRCGYC	3 cut(s)	804, 1117, 1582
BssSI	CACGAG	1 cut(s)	1455
BssT1I	CCWWGG	1 cut(s)	726
Bst2BI	CACGAG	1 cut(s)	1455
Bst2UI	CCWGG	3 cut(s)	321, 399, 408
Bst4CI	ACNGT	6 cut(s)	83, 122, 131, 603, 835, 1216
Bst6I	CTCTTC	3 cut(s)	662, 1070, 1476
BstACI	GRCGYC	3 cut(s)	804, 1117, 1582
BstAFI	CTTAAG	1 cut(s)	1462
BstBAI	YACGTR	1 cut(s)	1154
BstBI	TTCGAA	1 cut(s)	431
BstDEI	CTNAG	9 cut(s)	225, 240, 251, 281, 780, 1002, 1108, 1302, 1599
BstDSI	CCRYGG	1 cut(s)	1609
BstENI	CCTNNNNNAGG	2 cut(s)	779, 1005
BstF5I	GGATG	4 cut(s)	769, 1482, 1561, 1693
BstH2I	RGCGCY	2 cut(s)	1014, 1120
BstHHI	GCGC	3 cut(s)	1013, 1119, 1409
BstKTI	GATC	3 cut(s)	307, 455, 1685
BstMAI	GTCTC	2 cut(s)	205, 288
BstMBI	GATC	3 cut(s)	304, 452, 1682
BstMCI	CGRYCG	1 cut(s)	370
BstMWI	GCNNNNNNNGC	5 cut(s)	101, 638, 973, 1193, 1430
BstNI	CCWGG	3 cut(s)	321, 399, 408
BstSCI	CCNGG	3 cut(s)	319, 397, 406
BstSFI	CTRYAG	1 cut(s)	627
BstV1I	GCAGC	8 cut(s)	20, 223, 325, 641, 844, 866, 1377, 1539
BstV2I	GAAGAC	1 cut(s)	713
BstX2I	RGATCY	2 cut(s)	304, 452
BstYI	RGATCY	2 cut(s)	304, 452
BsuI	GTATCC	1 cut(s)	95
BsuRI	GGCC	1 cut(s)	5
BtgI	CCRYGG	1 cut(s)	1609
BtsCI	GGATG	4 cut(s)	769, 1482, 1561, 1693
BtsI	GCAGTG	3 cut(s)	624, 1231, 1409
BtsIMutI	CAGTG	6 cut(s)	118, 361, 555, 624, 1231, 1409
BveI	ACCTGC	1 cut(s)	1309
CfoI	GCGC	3 cut(s)	1013, 1119, 1409
Cfr13I	GGNCC	3 cut(s)	351, 404, 689
CseI	GACGC	1 cut(s)	1571
Csp6I	GTAC	6 cut(s)	542, 998, 1151, 1369, 1501, 1521
CviAII	CATG	3 cut(s)	1121, 1162, 1489
CviQI	GTAC	6 cut(s)	542, 998, 1151, 1369, 1501, 1521
DdeI	CTNAG	9 cut(s)	225, 240, 251, 281, 780, 1002, 1108, 1302, 1599
DinI	GGCGCC	1 cut(s)	1118
DpnI	GATC	3 cut(s)	306, 454, 1684
DpnII	GATC	3 cut(s)	304, 452, 1682
DriI	GACNNNNNGTC	1 cut(s)	808
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	3 cut(s)	662, 1070, 1476
Eam1105I	GACNNNNNGTC	1 cut(s)	808
EarI	CTCTTC	3 cut(s)	662, 1070, 1476
Ecl136II	GAGCTC	2 cut(s)	285, 1469
Eco130I	CCWWGG	1 cut(s)	726
Eco24I	GRGCYC	2 cut(s)	287, 1471
Eco31I	GGTCTC	1 cut(s)	288
Eco47I	GGWCC	3 cut(s)	351, 404, 689
Eco53kI	GAGCTC	2 cut(s)	285, 1469
Eco57I	CTGAAG	1 cut(s)	1719
EcoICRI	GAGCTC	2 cut(s)	285, 1469
EcoNI	CCTNNNNNAGG	2 cut(s)	779, 1005
EcoRI	GAATTC	1 cut(s)	427
EcoRII	CCWGG	3 cut(s)	319, 397, 406
EcoT14I	CCWWGG	1 cut(s)	726
EcoT38I	GRGCYC	2 cut(s)	287, 1471
EgeI	GGCGCC	1 cut(s)	1118
EheI	GGCGCC	1 cut(s)	1118
ErhI	CCWWGG	1 cut(s)	726
Esp3I	CGTCTC	1 cut(s)	205
FaeI	CATG	3 cut(s)	1124, 1165, 1492
FaqI	GGGAC	1 cut(s)	702
FatI	CATG	3 cut(s)	1120, 1161, 1488
FauI	CCCGC	1 cut(s)	601
FauNDI	CATATG	1 cut(s)	1070
FblI	GTMKAC	2 cut(s)	891, 1089
FokI	GGATG	4 cut(s)	776, 1469, 1568, 1700
FriOI	GRGCYC	2 cut(s)	287, 1471
FspBI	CTAG	5 cut(s)	662, 1014, 1026, 1425, 1475
GlaI	GCGC	3 cut(s)	1012, 1118, 1408
GsuI	CTGGAG	1 cut(s)	1614
HaeII	RGCGCY	2 cut(s)	1014, 1120
HaeIII	GGCC	1 cut(s)	5
HapII	CCGG	2 cut(s)	302, 1511
HgaI	GACGC	1 cut(s)	1571
HhaI	GCGC	3 cut(s)	1013, 1119, 1409
Hin1I	GRCGYC	3 cut(s)	804, 1117, 1582
Hin1II	CATG	3 cut(s)	1124, 1165, 1492
Hin6I	GCGC	3 cut(s)	1011, 1117, 1407
HinP1I	GCGC	3 cut(s)	1011, 1117, 1407
HincII	GTYRAC	2 cut(s)	892, 1326
HindII	GTYRAC	2 cut(s)	892, 1326
HpaII	CCGG	2 cut(s)	302, 1511
HphI	GGTGA	3 cut(s)	119, 335, 1043
Hpy166II	GTNNAC	5 cut(s)	559, 877, 892, 1090, 1326
Hpy188I	TCNGA	8 cut(s)	142, 243, 282, 469, 477, 1111, 1642, 1687
Hpy8I	GTNNAC	5 cut(s)	559, 877, 892, 1090, 1326
Hpy99I	CGWCG	2 cut(s)	809, 1587
HpyAV	CCTTC	5 cut(s)	901, 941, 974, 1565, 1571
HpyCH4III	ACNGT	6 cut(s)	83, 122, 131, 603, 835, 1216
HpyCH4IV	ACGT	4 cut(s)	26, 214, 804, 1153
HpyCH4V	TGCA	8 cut(s)	629, 767, 839, 854, 860, 1196, 1318, 1393
HpyF10VI	GCNNNNNNNGC	5 cut(s)	101, 638, 973, 1193, 1430
HpyF3I	CTNAG	9 cut(s)	225, 240, 251, 281, 780, 1002, 1108, 1302, 1599
HpySE526I	ACGT	4 cut(s)	26, 214, 804, 1153
Hsp92I	GRCGYC	3 cut(s)	804, 1117, 1582
Hsp92II	CATG	3 cut(s)	1124, 1165, 1492
HspAI	GCGC	3 cut(s)	1011, 1117, 1407
KasI	GGCGCC	1 cut(s)	1116
Kpn2I	TCCGGA	1 cut(s)	1510
Kzo9I	GATC	3 cut(s)	304, 452, 1682
LguI	GCTCTTC	1 cut(s)	1476
LmnI	GCTCC	3 cut(s)	1150, 1595, 1619
Lsp1109I	GCAGC	8 cut(s)	20, 223, 325, 641, 844, 866, 1377, 1539
LweI	GCATC	3 cut(s)	754, 1205, 1491
MaeI	CTAG	5 cut(s)	662, 1014, 1026, 1425, 1475
MaeII	ACGT	4 cut(s)	26, 214, 804, 1153
MaeIII	GTNAC	5 cut(s)	410, 671, 1031, 1177, 1226
MalI	GATC	3 cut(s)	306, 454, 1684
MbiI	CCGCTC	1 cut(s)	95
MboI	GATC	3 cut(s)	304, 452, 1682
MfeI	CAATTG	1 cut(s)	987
MflI	RGATCY	2 cut(s)	304, 452
MhlI	GDGCHC	4 cut(s)	287, 1147, 1456, 1471
MluCI	AATT	7 cut(s)	149, 162, 217, 427, 987, 1256, 1648
Mly113I	GGCGCC	1 cut(s)	1117
MlyI	GAGTC	3 cut(s)	772, 818, 1487
MmeI	TCCRAC	2 cut(s)	500, 1243
MroI	TCCGGA	1 cut(s)	1510
MroXI	GAANNNNTTC	2 cut(s)	509, 951
MseI	TTAA	5 cut(s)	14, 345, 360, 921, 1463
MslI	CAYNNNNRTG	1 cut(s)	1487
MspA1I	CMGCKG	3 cut(s)	255, 857, 1190
MspCI	CTTAAG	1 cut(s)	1462
MspI	CCGG	2 cut(s)	302, 1511
MspR9I	CCNGG	3 cut(s)	321, 399, 408
MunI	CAATTG	1 cut(s)	987
MvaI	CCWGG	3 cut(s)	321, 399, 408
MwoI	GCNNNNNNNGC	5 cut(s)	101, 638, 973, 1193, 1430
NarI	GGCGCC	1 cut(s)	1117
NdeI	CATATG	1 cut(s)	1070
NdeII	GATC	3 cut(s)	304, 452, 1682
NlaIII	CATG	3 cut(s)	1124, 1165, 1492
NlaIV	GGNNCC	4 cut(s)	352, 454, 690, 1118
NmuCI	GTSAC	2 cut(s)	1031, 1226
NspV	TTCGAA	1 cut(s)	431
PaqCI	CACCTGC	1 cut(s)	1309
PciSI	GCTCTTC	1 cut(s)	1476
PdmI	GAANNNNTTC	2 cut(s)	509, 951
PfeI	GAWTC	7 cut(s)	587, 1124, 1249, 1507, 1603, 1658, 1714
PleI	GAGTC	3 cut(s)	772, 817, 1487
PluTI	GGCGCC	1 cut(s)	1120
PpsI	GAGTC	3 cut(s)	772, 817, 1487
Ppu21I	YACGTR	1 cut(s)	1154
PsiI	TTATAA	1 cut(s)	846
Psp124BI	GAGCTC	2 cut(s)	287, 1471
Psp6I	CCWGG	3 cut(s)	319, 397, 406
PspGI	CCWGG	3 cut(s)	319, 397, 406
PspN4I	GGNNCC	4 cut(s)	352, 454, 690, 1118
PspPI	GGNCC	3 cut(s)	351, 404, 689
PsrI	GAACNNNNNNTAC	2 cut(s)	115, 147
PstI	CTGCAG	1 cut(s)	631
PsuI	RGATCY	2 cut(s)	304, 452
PvuII	CAGCTG	1 cut(s)	857
RsaI	GTAC	6 cut(s)	543, 999, 1152, 1370, 1502, 1522
RsaNI	GTAC	6 cut(s)	542, 998, 1151, 1369, 1501, 1521
RseI	CAYNNNNRTG	1 cut(s)	1487
SacI	GAGCTC	2 cut(s)	287, 1471
SalI	GTCGAC	1 cut(s)	890
SapI	GCTCTTC	1 cut(s)	1476
SaqAI	TTAA	5 cut(s)	14, 345, 360, 921, 1463
Sau3AI	GATC	3 cut(s)	304, 452, 1682
Sau96I	GGNCC	3 cut(s)	351, 404, 689
SchI	GAGTC	3 cut(s)	772, 818, 1487
ScrFI	CCNGG	3 cut(s)	321, 399, 408
SduI	GDGCHC	4 cut(s)	287, 1147, 1456, 1471
SfaNI	GCATC	3 cut(s)	754, 1205, 1491
SfcI	CTRYAG	1 cut(s)	627
SfoI	GGCGCC	1 cut(s)	1118
SfuI	TTCGAA	1 cut(s)	431
SinI	GGWCC	3 cut(s)	351, 404, 689
SmiMI	CAYNNNNRTG	1 cut(s)	1487
SmlI	CTYRAG	1 cut(s)	1462
SmoI	CTYRAG	1 cut(s)	1462
Sse9I	AATT	7 cut(s)	149, 162, 217, 427, 987, 1256, 1648
SspDI	GGCGCC	1 cut(s)	1116
SspMI	CTAG	5 cut(s)	662, 1014, 1026, 1425, 1475
SstI	GAGCTC	2 cut(s)	287, 1471
StyD4I	CCNGG	3 cut(s)	319, 397, 406
StyI	CCWWGG	1 cut(s)	726
TaaI	ACNGT	6 cut(s)	83, 122, 131, 603, 835, 1216
TaiI	ACGT	4 cut(s)	29, 217, 807, 1156
TaqI	TCGA	9 cut(s)	20, 97, 196, 221, 425, 431, 807, 891, 1585
TaqII	GACCGA	1 cut(s)	356
TasI	AATT	7 cut(s)	149, 162, 217, 427, 987, 1256, 1648
TauI	GCSGC	3 cut(s)	8, 1190, 1417
TfiI	GAWTC	7 cut(s)	587, 1124, 1249, 1507, 1603, 1658, 1714
Tru1I	TTAA	5 cut(s)	14, 345, 360, 921, 1463
Tru9I	TTAA	5 cut(s)	14, 345, 360, 921, 1463
TscAI	CASTG	6 cut(s)	125, 361, 555, 631, 1231, 1416
TseFI	GTSAC	2 cut(s)	1031, 1226
TseI	GCWGC	8 cut(s)	8, 236, 313, 629, 854, 857, 1390, 1527
Tsp45I	GTSAC	2 cut(s)	1031, 1226
TspDTI	ATGAA	4 cut(s)	900, 1057, 1391, 1572
TspGWI	ACGGA	3 cut(s)	325, 429, 1598
TspRI	CASTG	6 cut(s)	125, 361, 555, 631, 1231, 1416
Vha464I	CTTAAG	1 cut(s)	1462
VpaK11BI	GGWCC	3 cut(s)	351, 404, 689
XagI	CCTNNNNNAGG	2 cut(s)	779, 1005
XapI	RAATTY	4 cut(s)	149, 162, 427, 1256
XbaI	TCTAGA	1 cut(s)	1474
XcmI	CCANNNNNNNNNTGG	1 cut(s)	306
XmiI	GTMKAC	2 cut(s)	891, 1089
XmnI	GAANNNNTTC	2 cut(s)	509, 951
XspI	CTAG	5 cut(s)	662, 1014, 1026, 1425, 1475
ZraI	GACGTC	1 cut(s)	805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.