Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0013903

Physical Location & Seq

Genomic Coordinates Forward (+)

31075872 .. 31080977

Gene Structure 5106 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1218600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGCCGCGGCGCTCAGATCCAAGCCCTCCAGAGACACGGCGTCGTCCGCCGTCTCACAATTCAGGTACTTTGTCTTCAACTACATGCACGCCGGCAGGGTGGCCAGCTCTCACCATGCCCACCGCACCAAAAGCGACGACTTGTTCATGAACACTCCCTACCACTTCACTTCCTTCAAGCCGGTGTTGCCCGGCGGCAACTTTGTTGAAAAGGGTTCGCAGATTTTCAATCACCCGCGAGCTTCCCAGAGAGTTAGCAAGAATAGTGATAAGAATTGGGGACGGAAATTGAGCAGCAGCAGGTGTAACACTGTTGTGTCGTCTCAGGGGGACCCGCCGGAGGTGTGGTCCGGTGATGGAATTGTGGTTCGGACTGCTCCCGGCTCGAATTTGGTTCGTGGAGGAGGCGGTGGAGGCGGTCCGAGCCCTGGTTCCGGAGGTGGTTTCGATTCGAATTCGAAGGATGAGAATTGGGGTGGTTCCAATTTGGGGAATAATTTTCCTACTCCGAAGGAAATTTGTCAGGGGCTTGACACGTTTGTAATTGGGCAAGAGAAGGCCAAGAAGGTGCTATCTGTTGCTGTTTACAATCACTATAAGAGGATATATCATGAGTCCCTGCAGAAGTGGTCCACAGGAGATTCAAGTAACGGGAAAGTAGATGCAATGGATGACGATAGAGTGGAGCTTGAAAAAAGTAACATTCTTTTAATGGGGCCAACAGGCTCAGGGAAGACACTACTCGCCAAAACCTTGGCACGGTTTGTGAATGTTCCATTTGTTATTGCAGATGCGACAACATTAACTCAGGCAGGATATGTAGGGGAAGATGTGGAATCAATTTTATACAAACTCCTTACGGTTGCCGATTATAATGTAACAGCTGCACAGCAGGGAATTGTTTATATTGATGAAGTTGACAAGATTACCAAAAAGGCTGAGAGCCTAAATATTAGTAGGGATGTATCTGGAGAGGGTGTTCAACAGGCTTTACTTAAGATGCTGGAAGGGACCGTTGTCAATGTTCCTGAGAAGGGAGCTCGAAAGCACCCTAGAGGTGAAAACATTCAGATTGACACAAAGGATATTCTCTTCATATGTGGGGGTGCCTTTATTGACTTAGAGAAGACAATCTCAGAAAGACGCCAAGATTCCTCTATTGGGTTTGGTGCCCCTGTTAGGGCAAATATGAGGACCGGCGGGGTTACAGATGCTGCTGTTACATCATCCTTATTGGAGATTGTTGAAAGTAGTGATCTTATACGATATGGGTTGATACCAGAATTTGTTGGCCGGTTTCCCATCCTTGTCAGCTTGTCAGCACTAACCGAAAATCAATTGGTTGAGGTCTTGACAGAACCAAAAAATGCCCTCGGGAAGCAATACAAGAAGATGTTCCGAATGAATGGAGTCAAACTGCATTTCACAGAAAGTGCCTTGAGATTAATTGCTAGAAAGGCAATATCAAAAAACACCGGGGCTCGTGGCCTACGAGCAATTTTAGAAAATATATTGATGGATGCCATGTATGAGATTCCTGAAGTTAGAACAGGCGATGACATAATAGACGCAGTTGTAATTGATGAAGAAGCCGTTGGATTGGACGGCAAGGGTTGTGGAGCTAAAATCCTTTATGGCAAGGGTGCATTGGATCGCTATCATTCGCAGAATAAGGCGAAGGATGCAGAGATTGCAACCACGGAGGGTTCCGATGGAGAGCCGGAGGCGGAAACAGAGCTTTCTTCCGTTGTTGCTAGCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019538 GO:0030163 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042623 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0070011 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04112 map04112

Pfam Domains

Protein Families

AAA_2 ClpB_D2-small

Protein Analysis

588

Amino Acids

63.4

Weight (kDa)

6.63

Isoelectric Point (pI)

34.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_2	PF07724	231 - 435	4.2e-30	AAA domain (Cdc48 subfamily)
AAA	PF00004	235 - 341	2e-14	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	442 - 511	3.4e-10	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013903)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53350
fragaria_vesca	FvH4_7g31580
malus_domestica	MD01G1218600.v1.1 MD07G1288500.v1.1
prunus_persica	Prupe.2G310000_v2.0.a1
pyrus_communis	pycom01g22850 pycom07g26110
rosa_chinensis	RchiOBHm_Chr1g0380581
rosa_laevigata	RLG00000026283
rosa_multiflora	Rmu_ssc0000015.1_g000011
rosa_roxburghii	Rroxscaffold_4G00279170
rosa_rugosa	Rorug01G0422700
rosa_samantha	Rh1AG447200 Rh1BG402500 Rh1CG417000 Rh1DG432700
rosa_wichuraiana	Rw1G038900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	873
AarI	CACCTGC	1 cut(s)	291
Acc36I	ACCTGC	1 cut(s)	291
AccB1I	GGYRCC	2 cut(s)	1106, 1169
AccII	CGCG	2 cut(s)	8, 238
AccIII	TCCGGA	1 cut(s)	434
AclWI	GGATC	2 cut(s)	12, 1659
AcoI	YGGCCR	3 cut(s)	3, 102, 1291
AcsI	RAATTY	4 cut(s)	388, 454, 516, 1283
AcuI	CTGAAG	1 cut(s)	1560
AcyI	GRCGYC	2 cut(s)	41, 1144
AfaI	GTAC	1 cut(s)	68
AfiI	CCNNNNNNNGG	9 cut(s)	340, 428, 434, 489, 759, 1034, 1161, 1179, 1180
AflII	CTTAAG	1 cut(s)	995
AflIII	ACRYGT	1 cut(s)	534
AgsI	TTSAA	8 cut(s)	79, 178, 209, 229, 645, 692, 983, 1247
AhdI	GACNNNNNGTC	1 cut(s)	40
AjnI	CCWGG	1 cut(s)	427
AjuI	GAANNNNNNNTTGG	2 cut(s)	1578, 1610
AleI	CACNNNNGTG	1 cut(s)	314
AloI	GAACNNNNNNTCC	4 cut(s)	351, 383, 963, 995
AluBI	AGCT	8 cut(s)	108, 242, 688, 884, 1040, 1314, 1622, 1738
AluI	AGCT	8 cut(s)	108, 242, 688, 884, 1040, 1314, 1622, 1738
Alw21I	GWGCWC	1 cut(s)	1042
Alw26I	GTCTC	3 cut(s)	27, 58, 327
AlwI	GGATC	2 cut(s)	12, 1659
AlwNI	CAGNNNCTG	1 cut(s)	1214
Ama87I	CYCGRG	1 cut(s)	1373
Aor13HI	TCCGGA	1 cut(s)	434
AoxI	GGCC	6 cut(s)	3, 102, 558, 716, 1291, 1486
ApeKI	GCWGC	4 cut(s)	294, 297, 884, 1214
ApoI	RAATTY	4 cut(s)	388, 454, 516, 1283
AseI	ATTAAT	1 cut(s)	1445
Asp700I	GAANNNNTTC	2 cut(s)	1065, 1394
AspLEI	GCGC	1 cut(s)	13
AspS9I	GGNCC	7 cut(s)	331, 348, 419, 630, 716, 1011, 1194
AsuC2I	CCSGG	3 cut(s)	192, 381, 1477
AsuHPI	GGTGA	4 cut(s)	104, 224, 365, 1070
AsuII	TTCGAA	2 cut(s)	452, 458
AsuNHI	GCTAGC	1 cut(s)	1754
AvaI	CYCGRG	1 cut(s)	1373
AvaII	GGWCC	6 cut(s)	331, 348, 419, 630, 1011, 1194
BaeGI	GKGCMC	1 cut(s)	1174
BalI	TGGCCA	1 cut(s)	104
BanI	GGYRCC	2 cut(s)	1106, 1169
BanII	GRGCYC	3 cut(s)	428, 1042, 1483
BarI	GAAGNNNNNNTAC	2 cut(s)	59, 91
BauI	CACGAG	1 cut(s)	1482
BbsI	GAAGAC	3 cut(s)	67, 740, 1133
Bbv12I	GWGCWC	1 cut(s)	1042
BbvI	GCAGC	4 cut(s)	306, 309, 871, 1201
BccI	CCATC	4 cut(s)	350, 1310, 1510, 1706
BceAI	ACGGC	4 cut(s)	35, 54, 1577, 1621
BciT130I	CCWGG	1 cut(s)	429
BcnI	CCSGG	3 cut(s)	192, 381, 1477
BcoDI	GTCTC	3 cut(s)	27, 58, 327
BfaI	CTAG	3 cut(s)	1053, 1452, 1755
BfmI	CTRYAG	1 cut(s)	620
BfoI	RGCGCY	1 cut(s)	14
BfrI	CTTAAG	1 cut(s)	995
BfuAI	ACCTGC	1 cut(s)	291
BisI	GCNGC	7 cut(s)	6, 9, 196, 295, 298, 885, 1215
BlsI	GCNGC	7 cut(s)	7, 10, 197, 296, 299, 886, 1216
Bme1390I	CCNGG	4 cut(s)	192, 381, 429, 1477
Bme18I	GGWCC	6 cut(s)	331, 348, 419, 630, 1011, 1194
BmeRI	GACNNNNNGTC	1 cut(s)	40
BmeT110I	CYCGRG	1 cut(s)	1373
BmgT120I	GGNCC	7 cut(s)	331, 348, 419, 630, 716, 1011, 1194
BmiI	GGNNCC	9 cut(s)	332, 333, 433, 481, 717, 1012, 1108, 1171, 1708
BmrFI	CCNGG	4 cut(s)	192, 381, 429, 1477
BmsI	GCATC	6 cut(s)	652, 781, 990, 1201, 1510, 1672
BmtI	GCTAGC	1 cut(s)	1758
BoxI	GACNNNNGTC	1 cut(s)	1016
BpiI	GAAGAC	3 cut(s)	67, 740, 1133
BpmI	CTGGAG	2 cut(s)	13, 990
Bpu10I	CCTNAGC	1 cut(s)	727
Bpu14I	TTCGAA	2 cut(s)	452, 458
BpuEI	CTTGAG	1 cut(s)	1459
BpuMI	CCSGG	3 cut(s)	192, 381, 1477
BsaBI	GATNNNNATC	1 cut(s)	1656
BsaHI	GRCGYC	2 cut(s)	41, 1144
BsaJI	CCNNGG	6 cut(s)	6, 427, 753, 1372, 1476, 1698
BsaWI	WCCGGW	2 cut(s)	350, 434
BsaXI	ACNNNNNCTCC	4 cut(s)	332, 362, 963, 993
Bsc4I	CCNNNNNNNGG	9 cut(s)	340, 428, 434, 489, 759, 1034, 1161, 1179, 1180
Bse118I	RCCGGY	4 cut(s)	92, 181, 1196, 1293
Bse3DI	GCAATG	1 cut(s)	672
Bse8I	GATNNNNATC	1 cut(s)	1656
BseAI	TCCGGA	1 cut(s)	434
BseBI	CCWGG	1 cut(s)	429
BseDI	CCNNGG	6 cut(s)	6, 427, 753, 1372, 1476, 1698
BseGI	GGATG	7 cut(s)	469, 676, 967, 1226, 1302, 1525, 1687
BseJI	GATNNNNATC	1 cut(s)	1656
BseLI	CCNNNNNNNGG	9 cut(s)	340, 428, 434, 489, 759, 1034, 1161, 1179, 1180
BseMI	GCAATG	1 cut(s)	672
BseMII	CTCAG	7 cut(s)	28, 338, 741, 821, 930, 1020, 1149
BseRI	GAGGAG	1 cut(s)	417
BseSI	GKGCMC	1 cut(s)	1174
BseXI	GCAGC	4 cut(s)	306, 309, 871, 1201
BsgI	GTGCAG	1 cut(s)	870
Bsh1236I	CGCG	2 cut(s)	8, 238
BshFI	GGCC	6 cut(s)	5, 104, 560, 718, 1293, 1488
BshNI	GGYRCC	2 cut(s)	1106, 1169
BsiHKAI	GWGCWC	1 cut(s)	1042
BsiHKCI	CYCGRG	1 cut(s)	1373
BslFI	GGGAC	4 cut(s)	294, 344, 601, 1024
BslI	CCNNNNNNNGG	9 cut(s)	340, 428, 434, 489, 759, 1034, 1161, 1179, 1180
BsmAI	GTCTC	3 cut(s)	27, 58, 327
BsmBI	CGTCTC	2 cut(s)	58, 327
BsmFI	GGGAC	4 cut(s)	294, 344, 601, 1024
BsnI	GGCC	6 cut(s)	5, 104, 560, 718, 1293, 1488
BsoBI	CYCGRG	1 cut(s)	1373
Bsp119I	TTCGAA	2 cut(s)	452, 458
Bsp1286I	GDGCHC	4 cut(s)	428, 1042, 1174, 1483
Bsp13I	TCCGGA	1 cut(s)	434
Bsp143I	GATC	3 cut(s)	17, 1255, 1651
BspANI	GGCC	6 cut(s)	5, 104, 560, 718, 1293, 1488
BspCNI	CTCAG	7 cut(s)	27, 337, 740, 820, 931, 1021, 1148
BspEI	TCCGGA	1 cut(s)	434
BspFNI	CGCG	2 cut(s)	8, 238
BspHI	TCATGA	2 cut(s)	147, 610
BspLI	GGNNCC	9 cut(s)	332, 333, 433, 481, 717, 1012, 1108, 1171, 1708
BspMAI	CTGCAG	1 cut(s)	624
BspMI	ACCTGC	1 cut(s)	291
BspOI	GCTAGC	1 cut(s)	1758
BspPI	GGATC	2 cut(s)	12, 1659
BspT104I	TTCGAA	2 cut(s)	452, 458
BspT107I	GGYRCC	2 cut(s)	1106, 1169
BspTI	CTTAAG	1 cut(s)	995
BsrDI	GCAATG	1 cut(s)	672
BsrFI	RCCGGY	4 cut(s)	92, 181, 1196, 1293
BssAI	RCCGGY	4 cut(s)	92, 181, 1196, 1293
BssECI	CCNNGG	6 cut(s)	6, 427, 753, 1372, 1476, 1698
BssMI	GATC	3 cut(s)	17, 1255, 1651
BssNI	GRCGYC	2 cut(s)	41, 1144
BssSI	CACGAG	1 cut(s)	1482
BssT1I	CCWWGG	1 cut(s)	753
Bst2BI	CACGAG	1 cut(s)	1482
Bst2UI	CCWGG	1 cut(s)	429
Bst4CI	ACNGT	4 cut(s)	313, 762, 862, 1015
Bst6I	CTCTTC	1 cut(s)	1097
BstACI	GRCGYC	2 cut(s)	41, 1144
BstAFI	CTTAAG	1 cut(s)	995
BstAPI	GCANNNNNTGC	1 cut(s)	1691
BstBI	TTCGAA	2 cut(s)	452, 458
BstC8I	GCNNGC	5 cut(s)	90, 94, 106, 240, 1756
BstDEI	CTNAG	8 cut(s)	14, 324, 727, 807, 939, 1029, 1120, 1135
BstDSI	CCRYGG	2 cut(s)	6, 1698
BstF5I	GGATG	7 cut(s)	469, 676, 967, 1226, 1302, 1525, 1687
BstFNI	CGCG	2 cut(s)	8, 238
BstH2I	RGCGCY	1 cut(s)	14
BstHHI	GCGC	1 cut(s)	13
BstKTI	GATC	3 cut(s)	20, 1258, 1654
BstMAI	GTCTC	3 cut(s)	27, 58, 327
BstMBI	GATC	3 cut(s)	17, 1255, 1651
BstMWI	GCNNNNNNNGC	8 cut(s)	47, 132, 187, 414, 423, 1457, 1682, 1691
BstNI	CCWGG	1 cut(s)	429
BstNSI	RCATGY	2 cut(s)	88, 1762
BstPAI	GACNNNNGTC	1 cut(s)	1016
BstSCI	CCNGG	4 cut(s)	190, 379, 427, 1475
BstSFI	CTRYAG	1 cut(s)	620
BstSLI	GKGCMC	1 cut(s)	1174
BstUI	CGCG	2 cut(s)	8, 238
BstV1I	GCAGC	4 cut(s)	306, 309, 871, 1201
BstV2I	GAAGAC	3 cut(s)	67, 740, 1133
BstX2I	RGATCY	1 cut(s)	17
BstXI	CCANNNNNNTGG	1 cut(s)	754
BstYI	RGATCY	1 cut(s)	17
BsuRI	GGCC	6 cut(s)	5, 104, 560, 718, 1293, 1488
BtgI	CCRYGG	2 cut(s)	6, 1698
BtgZI	GCGATG	1 cut(s)	1569
BtsCI	GGATG	7 cut(s)	469, 676, 967, 1226, 1302, 1525, 1687
BtsIMutI	CAGTG	1 cut(s)	309
BveI	ACCTGC	1 cut(s)	291
Cac8I	GCNNGC	5 cut(s)	90, 94, 106, 240, 1756
CaiI	CAGNNNCTG	1 cut(s)	1214
CciI	TCATGA	2 cut(s)	147, 610
CfoI	GCGC	1 cut(s)	13
Cfr10I	RCCGGY	4 cut(s)	92, 181, 1196, 1293
Cfr13I	GGNCC	7 cut(s)	331, 348, 419, 630, 716, 1011, 1194
Cfr42I	CCGCGG	1 cut(s)	9
CpoI	CGGWCCG	1 cut(s)	419
CseI	GACGC	3 cut(s)	30, 1152, 1577
Csp6I	GTAC	1 cut(s)	67
CspCI	CAANNNNNGTGG	2 cut(s)	1597, 1632
CspI	CGGWCCG	1 cut(s)	419
CviAII	CATG	6 cut(s)	85, 116, 148, 611, 1525, 1759
CviQI	GTAC	1 cut(s)	67
DdeI	CTNAG	8 cut(s)	14, 324, 727, 807, 939, 1029, 1120, 1135
DpnI	GATC	3 cut(s)	19, 1257, 1653
DpnII	GATC	3 cut(s)	17, 1255, 1651
DriI	GACNNNNNGTC	1 cut(s)	40
EaeI	YGGCCR	3 cut(s)	3, 102, 1291
Eam1104I	CTCTTC	1 cut(s)	1097
Eam1105I	GACNNNNNGTC	1 cut(s)	40
EarI	CTCTTC	1 cut(s)	1097
EciI	GGCGGA	2 cut(s)	37, 1742
Ecl136II	GAGCTC	1 cut(s)	1040
Eco130I	CCWWGG	1 cut(s)	753
Eco24I	GRGCYC	3 cut(s)	428, 1042, 1483
Eco47I	GGWCC	6 cut(s)	331, 348, 419, 630, 1011, 1194
Eco53kI	GAGCTC	1 cut(s)	1040
Eco57I	CTGAAG	1 cut(s)	1560
Eco88I	CYCGRG	1 cut(s)	1373
EcoICRI	GAGCTC	1 cut(s)	1040
EcoO109I	RGGNCCY	1 cut(s)	331
EcoRI	GAATTC	1 cut(s)	454
EcoRII	CCWGG	1 cut(s)	427
EcoT14I	CCWWGG	1 cut(s)	753
EcoT38I	GRGCYC	3 cut(s)	428, 1042, 1483
ErhI	CCWWGG	1 cut(s)	753
Esp3I	CGTCTC	2 cut(s)	58, 327
FaeI	CATG	6 cut(s)	88, 119, 151, 614, 1528, 1762
FaqI	GGGAC	4 cut(s)	294, 344, 601, 1024
FatI	CATG	6 cut(s)	84, 115, 147, 610, 1524, 1758
FauI	CCCGC	3 cut(s)	243, 342, 1193
FauNDI	CATATG	1 cut(s)	1097
Fnu4HI	GCNGC	7 cut(s)	6, 9, 196, 295, 298, 885, 1215
FokI	GGATG	7 cut(s)	476, 683, 974, 1213, 1289, 1532, 1694
FriOI	GRGCYC	3 cut(s)	428, 1042, 1483
Fsp4HI	GCNGC	7 cut(s)	6, 9, 196, 295, 298, 885, 1215
FspBI	CTAG	3 cut(s)	1053, 1452, 1755
GlaI	GCGC	1 cut(s)	12
GluI	GCNGC	7 cut(s)	6, 9, 196, 295, 298, 885, 1215
GsuI	CTGGAG	2 cut(s)	13, 990
HaeII	RGCGCY	1 cut(s)	14
HaeIII	GGCC	6 cut(s)	5, 104, 560, 718, 1293, 1488
HgaI	GACGC	3 cut(s)	30, 1152, 1577
HhaI	GCGC	1 cut(s)	13
Hin1I	GRCGYC	2 cut(s)	41, 1144
Hin1II	CATG	6 cut(s)	88, 119, 151, 614, 1528, 1762
Hin6I	GCGC	1 cut(s)	11
HinP1I	GCGC	1 cut(s)	11
HincII	GTYRAC	1 cut(s)	919
HindII	GTYRAC	1 cut(s)	919
HinfI	GANTC	7 cut(s)	449, 614, 641, 836, 1151, 1410, 1534
HphI	GGTGA	4 cut(s)	104, 224, 365, 1070
Hpy166II	GTNNAC	3 cut(s)	586, 633, 919
Hpy188I	TCNGA	8 cut(s)	17, 372, 423, 510, 1071, 1138, 1400, 1711
Hpy188III	TCNNGA	9 cut(s)	30, 148, 435, 611, 969, 1028, 1351, 1375, 1538
Hpy8I	GTNNAC	3 cut(s)	586, 633, 919
Hpy99I	CGWCG	2 cut(s)	46, 140
HpyAV	CCTTC	8 cut(s)	184, 454, 505, 550, 559, 1001, 1027, 1672
HpyCH4III	ACNGT	4 cut(s)	313, 762, 862, 1015
HpyCH4IV	ACGT	1 cut(s)	536
HpyCH4V	TGCA	9 cut(s)	88, 622, 665, 788, 887, 1420, 1646, 1685, 1694
HpyF10VI	GCNNNNNNNGC	8 cut(s)	47, 132, 187, 414, 423, 1457, 1682, 1691
HpyF3I	CTNAG	8 cut(s)	14, 324, 727, 807, 939, 1029, 1120, 1135
HpySE526I	ACGT	1 cut(s)	536
Hsp92I	GRCGYC	2 cut(s)	41, 1144
Hsp92II	CATG	6 cut(s)	88, 119, 151, 614, 1528, 1762
HspAI	GCGC	1 cut(s)	11
KflI	GGGWCCC	1 cut(s)	331
Kpn2I	TCCGGA	1 cut(s)	434
KroI	GCCGGC	1 cut(s)	92
KroNI	GCCGGC	1 cut(s)	94
KspI	CCGCGG	1 cut(s)	9
Kzo9I	GATC	3 cut(s)	17, 1255, 1651
LmnI	GCTCC	4 cut(s)	382, 685, 1037, 1619
Lsp1109I	GCAGC	4 cut(s)	306, 309, 871, 1201
LweI	GCATC	6 cut(s)	652, 781, 990, 1201, 1510, 1672
MaeI	CTAG	3 cut(s)	1053, 1452, 1755
MaeII	ACGT	1 cut(s)	536
MaeIII	GTNAC	6 cut(s)	305, 647, 698, 877, 1204, 1219
MalI	GATC	3 cut(s)	19, 1257, 1653
MboI	GATC	3 cut(s)	17, 1255, 1651
MboII	GAAGA	8 cut(s)	67, 745, 839, 1084, 1138, 1402, 1598, 1734
MfeI	CAATTG	1 cut(s)	1337
MflI	RGATCY	1 cut(s)	17
MhlI	GDGCHC	4 cut(s)	428, 1042, 1174, 1483
MlsI	TGGCCA	1 cut(s)	104
MluNI	TGGCCA	1 cut(s)	104
MlyI	GAGTC	2 cut(s)	623, 1419
MmeI	TCCRAC	1 cut(s)	1576
Mox20I	TGGCCA	1 cut(s)	104
MroI	TCCGGA	1 cut(s)	434
MroNI	GCCGGC	1 cut(s)	92
MroXI	GAANNNNTTC	2 cut(s)	1065, 1394
MscI	TGGCCA	1 cut(s)	104
MseI	TTAA	4 cut(s)	710, 803, 996, 1445
MslI	CAYNNNNRTG	1 cut(s)	314
Msp20I	TGGCCA	1 cut(s)	104
MspA1I	CMGCKG	2 cut(s)	8, 884
MspCI	CTTAAG	1 cut(s)	995
MspR9I	CCNGG	4 cut(s)	192, 381, 429, 1477
MunI	CAATTG	1 cut(s)	1337
MvaI	CCWGG	1 cut(s)	429
MvnI	CGCG	2 cut(s)	8, 238
MwoI	GCNNNNNNNGC	8 cut(s)	47, 132, 187, 414, 423, 1457, 1682, 1691
NaeI	GCCGGC	1 cut(s)	94
NciI	CCSGG	3 cut(s)	192, 381, 1477
NdeI	CATATG	1 cut(s)	1097
NdeII	GATC	3 cut(s)	17, 1255, 1651
NgoMIV	GCCGGC	1 cut(s)	92
NheI	GCTAGC	1 cut(s)	1754
NlaIII	CATG	6 cut(s)	88, 119, 151, 614, 1528, 1762
NlaIV	GGNNCC	9 cut(s)	332, 333, 433, 481, 717, 1012, 1108, 1171, 1708
NspI	RCATGY	2 cut(s)	88, 1762
NspV	TTCGAA	2 cut(s)	452, 458
OliI	CACNNNNGTG	1 cut(s)	314
PagI	TCATGA	2 cut(s)	147, 610
PaqCI	CACCTGC	1 cut(s)	291
PcsI	WCGNNNNNNNCGW	1 cut(s)	1489
PdiI	GCCGGC	1 cut(s)	94
PdmI	GAANNNNTTC	2 cut(s)	1065, 1394
PfeI	GAWTC	5 cut(s)	449, 641, 836, 1151, 1534
PkrI	GCNGC	7 cut(s)	7, 10, 197, 296, 299, 886, 1216
PleI	GAGTC	2 cut(s)	622, 1418
PpsI	GAGTC	2 cut(s)	622, 1418
PpuMI	RGGWCCY	1 cut(s)	331
PshAI	GACNNNNGTC	1 cut(s)	1016
PshBI	ATTAAT	1 cut(s)	1445
PsiI	TTATAA	1 cut(s)	873
Psp124BI	GAGCTC	1 cut(s)	1042
Psp5II	RGGWCCY	1 cut(s)	331
Psp6I	CCWGG	1 cut(s)	427
PspGI	CCWGG	1 cut(s)	427
PspN4I	GGNNCC	9 cut(s)	332, 333, 433, 481, 717, 1012, 1108, 1171, 1708
PspPI	GGNCC	7 cut(s)	331, 348, 419, 630, 716, 1011, 1194
PspPPI	RGGWCCY	1 cut(s)	331
PsrI	GAACNNNNNNTAC	2 cut(s)	143, 175
PstI	CTGCAG	1 cut(s)	624
PstNI	CAGNNNCTG	1 cut(s)	1214
PsuI	RGATCY	1 cut(s)	17
PvuII	CAGCTG	1 cut(s)	884
RsaI	GTAC	1 cut(s)	68
RsaNI	GTAC	1 cut(s)	67
RseI	CAYNNNNRTG	1 cut(s)	314
Rsr2I	CGGWCCG	1 cut(s)	419
RsrII	CGGWCCG	1 cut(s)	419
SacI	GAGCTC	1 cut(s)	1042
SacII	CCGCGG	1 cut(s)	9
SaqAI	TTAA	4 cut(s)	710, 803, 996, 1445
SatI	GCNGC	7 cut(s)	6, 9, 196, 295, 298, 885, 1215
Sau3AI	GATC	3 cut(s)	17, 1255, 1651
Sau96I	GGNCC	7 cut(s)	331, 348, 419, 630, 716, 1011, 1194
SchI	GAGTC	2 cut(s)	623, 1419
ScrFI	CCNGG	4 cut(s)	192, 381, 429, 1477
SduI	GDGCHC	4 cut(s)	428, 1042, 1174, 1483
SfaNI	GCATC	6 cut(s)	652, 781, 990, 1201, 1510, 1672
SfcI	CTRYAG	1 cut(s)	620
Sfr303I	CCGCGG	1 cut(s)	9
SfuI	TTCGAA	2 cut(s)	452, 458
SgrBI	CCGCGG	1 cut(s)	9
SinI	GGWCC	6 cut(s)	331, 348, 419, 630, 1011, 1194
SmiMI	CAYNNNNRTG	1 cut(s)	314
SmlI	CTYRAG	2 cut(s)	995, 1438
SmoI	CTYRAG	2 cut(s)	995, 1438
SspI	AATATT	1 cut(s)	952
SspMI	CTAG	3 cut(s)	1053, 1452, 1755
SstI	GAGCTC	1 cut(s)	1042
StyD4I	CCNGG	4 cut(s)	190, 379, 427, 1475
StyI	CCWWGG	1 cut(s)	753
TaaI	ACNGT	4 cut(s)	313, 762, 862, 1015
TaiI	ACGT	1 cut(s)	539
TaqI	TCGA	5 cut(s)	386, 447, 452, 458, 1042
TauI	GCSGC	3 cut(s)	8, 11, 198
TfiI	GAWTC	5 cut(s)	449, 641, 836, 1151, 1534
Tru1I	TTAA	4 cut(s)	710, 803, 996, 1445
Tru9I	TTAA	4 cut(s)	710, 803, 996, 1445
TscAI	CASTG	1 cut(s)	316
TseI	GCWGC	4 cut(s)	294, 297, 884, 1214
TspDTI	ATGAA	6 cut(s)	136, 164, 927, 1084, 1418, 1599
TspGWI	ACGGA	3 cut(s)	298, 1715, 1735
TspRI	CASTG	1 cut(s)	316
Vha464I	CTTAAG	1 cut(s)	995
VpaK11BI	GGWCC	6 cut(s)	331, 348, 419, 630, 1011, 1194
VspI	ATTAAT	1 cut(s)	1445
XapI	RAATTY	4 cut(s)	388, 454, 516, 1283
XceI	RCATGY	2 cut(s)	88, 1762
XmnI	GAANNNNTTC	2 cut(s)	1065, 1394
XspI	CTAG	3 cut(s)	1053, 1452, 1755

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.