Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007564

Physical Location & Seq

Genomic Coordinates Reverse (-)

21649532 .. 21651713

Gene Structure 2182 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G53370.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGGTTACGACAGGCTCGGTCCATCCGGACCATCAAACCCGAACCAGAAGGACCCTGCCACGTCACTCCCGGAACTACAGAAGAAAACTAAAACGAAGCTGATTCTCTTCACTTTGGCCGTTTTGGTAGTTGGAGTAGTCTGCTTCGGTATCTTTGCCGGCATCCGAGCCGTAGACTCGGGTAAAACCGAGCCGAAACTAACCCGTAAACCGACCCAAGCAATCTCCCGAACCTGTAGCAAATCCTTATACCCGAACCTATGCATCGACACACTTCTTGACTTTCCGGGATCTTTAACCGCCGACGAGAACGAGCTCATTCACATATCATTCAACGCAACGCTTCAAAAATTCAGCAAAGCTCTTTATACTTCTTCGACGATCACATACACTCAGATGCCCCCACGTGTACGGTCAGCTTACGATTCTTGCCTTGAGTTACTTGATGATTCAGTGGACGCGCTCACACGCGCTCTTTCCTCCGTCGTTGTCGTCTCCGGAGATGAGTCTCATTCCGATGTGATGACGTGGCTTAGCTCTGCGATGACTAACCACGACACTTGCACTGACGGATTCGATGAAATTGAAGGTCAAGGAGGAGAAGTGAAGGATCAAGTGATCGGAGCGGTTAAAGATTTGTCGGAGATGGTGAGTAATTGTTTGGCTATATTCGCCGGAAAAGTTAAGGATTTATCTGGAGTTCCGGTGGTGAATAATAGGAAGTTACTTGGGACTGAAGAAACAGAGGAATTACCTAATTGGTTGAAGAGAGAAGACAGAGAGCTTCTTGGTACTCCAACGTCGGCGATTCAAGCTGATATCACGGTGTCGAAAGACGGTAGCGGAACGTTTAAGACGATCGCGGAGGCGATCAAGAAAGCCCCGGAGCATAGTAGCCGACGATTCGTCATCTACGTTAAAGCAGGAAGATACGAAGAAGAGAATCTGAAAGTTGGTAGGAAGAAGACAAACTTGATGTTCATCGGGGACGGCAAGGGTAAAACGGTCATAACCGGTGGGAAAAGTATCGCCGATGATCTAACTACTTTTCACACCGCCACTTTCGCTGCGACTGGTGCTGGATTTATAGTGAGGGACATGACGTTTGAGAATTACGCCGGACCGGCTAAGCATCAGGCCGTGGCACTCCGTGTTGGTGGAGACCACGCGGTGGTTTACCGTTGCAACATTATCGGTTACCAAGACGCGCTTTACGTACATTCTAACCGACAGTTTTTCCGCGAATGCGAAATTTATGGAACGGTCGATTTTATATTCGGGAATGCGGCTGTGATCTTACAGAGTTGTAACATTTATGCGCGTAAACCAATGGCTCAGCAGAAGATTACTATTACGGCTCAGAACCGAAAAGATCCGAATCAGAATACGGGGATTTCGATTCATGCTTGTAAGCTACTAGCAACACCGGATCTTGAAGCCTCTAAGGGTAGTTATCCGACGTATCTCGGCCGTCCGTGGAAGTTGTATTCTAGAGTTGTGTACATGATGTCGGATATGGGTGACCATATTGACCCGCGAGGATGGTTGGAGTGGAATGGTCCGTTTGCATTGGACTCGTTGTACTATGGTGAGTATATGAACAAAGGGTTGGGTTCAGGAATTGGTCAACGAGTCAAATGGCCTGGTTATCATGTTATTACCTCAACGGTAGAGGCTAGTAAGTTTACGGTGGCTCAGTTCATTTCTGGTTCTTCGTGGTTGCCATCCACCGGTGTGTCCTTCTTCTCCGGGTTGTCACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0010119 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050789 GO:0050790 GO:0050794 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

587

Amino Acids

64.24

Weight (kDa)

7.55

Isoelectric Point (pI)

34.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	110 - 259	2e-34	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	309 - 606	9.1e-147	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49220 AT3G49220 AT3G49220 AT5G53370 AT5G53370
fragaria_vesca	FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650
malus_domestica	MD01G1220700.v1.1 MD07G1289200.v1.1
prunus_persica	Prupe.2G310600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0380731
rosa_laevigata	RLG00000026273
rosa_multiflora	Rmu_sc0001637.1_g000012
rosa_roxburghii	Rroxscaffold_4G00279070
rosa_rugosa	Rorug01G0424000
rosa_samantha	Rh1AG448200 Rh1BG403600 Rh1CG417900 Rh1DG433900
rosa_wichuraiana	Rw0G005890 Rw1G039010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1171
AccBSI	CCGCTC	1 cut(s)	626
AccI	GTMKAC	1 cut(s)	174
AccII	CGCG	8 cut(s)	461, 471, 863, 1169, 1208, 1242, 1321, 1537
AccIII	TCCGGA	2 cut(s)	26, 497
AciI	CCGC	9 cut(s)	300, 626, 843, 863, 1056, 1169, 1240, 1286, 1535
AclI	AACGTT	1 cut(s)	848
AclWI	GGATC	4 cut(s)	298, 618, 1367, 1437
AcoI	YGGCCR	2 cut(s)	117, 1468
AcsI	RAATTY	2 cut(s)	350, 1251
AcuI	CTGAAG	1 cut(s)	756
AcvI	CACGTG	1 cut(s)	407
AdeI	CACNNNGTG	2 cut(s)	1151, 1171
AfaI	GTAC	5 cut(s)	411, 793, 1218, 1502, 1583
AfiI	CCNNNNNNNGG	2 cut(s)	1171, 1730
AflIII	ACRYGT	1 cut(s)	406
AgeI	ACCGGT	2 cut(s)	1013, 1730
AgsI	TTSAA	6 cut(s)	334, 347, 587, 766, 812, 1436
AhdI	GACNNNNNGTC	1 cut(s)	905
AjiI	CACGTC	2 cut(s)	63, 528
AjnI	CCWGG	1 cut(s)	1642
AjuI	GAANNNNNNNTTGG	2 cut(s)	1592, 1624
AleI	CACNNNNGTG	1 cut(s)	1733
AluBI	AGCT	8 cut(s)	100, 316, 362, 419, 537, 784, 815, 1414
AluI	AGCT	8 cut(s)	100, 316, 362, 419, 537, 784, 815, 1414
Alw21I	GWGCWC	1 cut(s)	318
Alw26I	GTCTC	3 cut(s)	499, 513, 1155
AlwI	GGATC	4 cut(s)	298, 618, 1367, 1437
Ama87I	CYCGRG	1 cut(s)	178
Aor13HI	TCCGGA	2 cut(s)	26, 497
AoxI	GGCC	4 cut(s)	117, 1137, 1468, 1640
ApeKI	GCWGC	1 cut(s)	1067
ApoI	RAATTY	2 cut(s)	350, 1251
ArsI	GACNNNNNNTTYG	2 cut(s)	1088, 1120
AsiGI	ACCGGT	2 cut(s)	1013, 1730
AspLEI	GCGC	4 cut(s)	463, 473, 1210, 1321
AspS9I	GGNCC	5 cut(s)	20, 29, 52, 1121, 1559
AsuC2I	CCSGG	4 cut(s)	71, 288, 884, 1750
AsuHPI	GGTGA	4 cut(s)	661, 721, 1532, 1601
AvaI	CYCGRG	1 cut(s)	178
AvaII	GGWCC	5 cut(s)	20, 29, 52, 1121, 1559
BanII	GRGCYC	1 cut(s)	318
BbrPI	CACGTG	1 cut(s)	407
BbsI	GAAGAC	2 cut(s)	780, 971
Bbv12I	GWGCWC	1 cut(s)	318
BbvI	GCAGC	1 cut(s)	1054
BccI	CCATC	5 cut(s)	31, 40, 640, 1536, 1732
BceAI	ACGGC	6 cut(s)	104, 155, 1006, 1124, 1371, 1455
BcgI	CGANNNNNNTGC	4 cut(s)	1174, 1208, 1557, 1591
BciT130I	CCWGG	1 cut(s)	1644
BcnI	CCSGG	4 cut(s)	71, 288, 884, 1750
BcoDI	GTCTC	3 cut(s)	499, 513, 1155
BfaI	CTAG	3 cut(s)	1418, 1491, 1677
BfmI	CTRYAG	2 cut(s)	77, 235
BglI	GCCNNNNNGGC	1 cut(s)	1124
BisI	GCNGC	2 cut(s)	1068, 1287
BlpI	GCTNAGC	3 cut(s)	533, 1128, 1335
BlsI	GCNGC	2 cut(s)	1069, 1288
Bme1390I	CCNGG	5 cut(s)	71, 288, 884, 1644, 1750
Bme18I	GGWCC	5 cut(s)	20, 29, 52, 1121, 1559
BmeRI	GACNNNNNGTC	1 cut(s)	905
BmeT110I	CYCGRG	1 cut(s)	178
BmgBI	CACGTC	2 cut(s)	63, 528
BmgT120I	GGNCC	5 cut(s)	20, 29, 52, 1121, 1559
BmiI	GGNNCC	1 cut(s)	54
BmrFI	CCNGG	5 cut(s)	71, 288, 884, 1644, 1750
BmsI	GCATC	4 cut(s)	171, 273, 387, 1141
BpiI	GAAGAC	2 cut(s)	780, 971
BpmI	CTGGAG	1 cut(s)	717
Bpu1102I	GCTNAGC	3 cut(s)	533, 1128, 1335
BpuEI	CTTGAG	1 cut(s)	455
BpuMI	CCSGG	4 cut(s)	71, 288, 884, 1750
BsaAI	YACGTR	2 cut(s)	407, 1216
BsaBI	GATNNNNATC	2 cut(s)	980, 1377
BsaI	GGTCTC	1 cut(s)	1155
BsaJI	CCNNGG	3 cut(s)	882, 1140, 1475
BsaWI	WCCGGW	6 cut(s)	26, 497, 703, 1013, 1426, 1730
BsaXI	ACNNNNNCTCC	4 cut(s)	588, 618, 690, 720
Bsc4I	CCNNNNNNNGG	2 cut(s)	1171, 1730
Bse118I	RCCGGY	4 cut(s)	158, 1013, 1123, 1730
Bse1I	ACTGG	1 cut(s)	1078
Bse8I	GATNNNNATC	2 cut(s)	980, 1377
BseAI	TCCGGA	2 cut(s)	26, 497
BseBI	CCWGG	1 cut(s)	1644
BseDI	CCNNGG	3 cut(s)	882, 1140, 1475
BseGI	GGATG	4 cut(s)	23, 162, 1547, 1724
BseJI	GATNNNNATC	2 cut(s)	980, 1377
BseLI	CCNNNNNNNGG	2 cut(s)	1171, 1730
BseMII	CTCAG	4 cut(s)	407, 1349, 1373, 1709
BseNI	ACTGG	1 cut(s)	1078
BseRI	GAGGAG	1 cut(s)	612
BseX3I	CGGCCG	1 cut(s)	1468
BseXI	GCAGC	1 cut(s)	1054
Bsh1236I	CGCG	8 cut(s)	461, 471, 863, 1169, 1208, 1242, 1321, 1537
Bsh1285I	CGRYCG	3 cut(s)	861, 1266, 1471
BshFI	GGCC	4 cut(s)	119, 1139, 1470, 1642
BshTI	ACCGGT	2 cut(s)	1013, 1730
BsiEI	CGRYCG	3 cut(s)	861, 1266, 1471
BsiHKAI	GWGCWC	1 cut(s)	318
BsiHKCI	CYCGRG	1 cut(s)	178
BslFI	GGGAC	3 cut(s)	745, 1001, 1109
BslI	CCNNNNNNNGG	2 cut(s)	1171, 1730
BsmAI	GTCTC	3 cut(s)	499, 513, 1155
BsmBI	CGTCTC	1 cut(s)	499
BsmFI	GGGAC	3 cut(s)	745, 1001, 1109
BsmI	GAATGC	2 cut(s)	1250, 1288
BsnI	GGCC	4 cut(s)	119, 1139, 1470, 1642
Bso31I	GGTCTC	1 cut(s)	1155
BsoBI	CYCGRG	1 cut(s)	178
Bsp1286I	GDGCHC	1 cut(s)	318
Bsp13I	TCCGGA	2 cut(s)	26, 497
Bsp1407I	TGTACA	1 cut(s)	1500
Bsp1720I	GCTNAGC	3 cut(s)	533, 1128, 1335
BspACI	CCGC	9 cut(s)	300, 626, 843, 863, 1056, 1169, 1240, 1286, 1535
BspANI	GGCC	4 cut(s)	119, 1139, 1470, 1642
BspCNI	CTCAG	4 cut(s)	406, 1348, 1372, 1708
BspEI	TCCGGA	2 cut(s)	26, 497
BspFNI	CGCG	8 cut(s)	461, 471, 863, 1169, 1208, 1242, 1321, 1537
BspLI	GGNNCC	1 cut(s)	54
BspPI	GGATC	4 cut(s)	298, 618, 1367, 1437
BspTNI	GGTCTC	1 cut(s)	1155
BsrBI	CCGCTC	1 cut(s)	626
BsrFI	RCCGGY	4 cut(s)	158, 1013, 1123, 1730
BsrGI	TGTACA	1 cut(s)	1500
BsrI	ACTGG	1 cut(s)	1078
BssAI	RCCGGY	4 cut(s)	158, 1013, 1123, 1730
BssECI	CCNNGG	3 cut(s)	882, 1140, 1475
Bst2UI	CCWGG	1 cut(s)	1644
Bst4CI	ACNGT	9 cut(s)	414, 826, 839, 1006, 1181, 1233, 1264, 1669, 1690
Bst6I	CTCTTC	3 cut(s)	113, 761, 933
BstAUI	TGTACA	1 cut(s)	1500
BstBAI	YACGTR	2 cut(s)	407, 1216
BstC8I	GCNNGC	1 cut(s)	160
BstDEI	CTNAG	7 cut(s)	393, 533, 1128, 1335, 1359, 1443, 1695
BstDSI	CCRYGG	2 cut(s)	1140, 1475
BstEII	GGTNACC	2 cut(s)	1196, 1520
BstF5I	GGATG	4 cut(s)	23, 162, 1547, 1724
BstFNI	CGCG	8 cut(s)	461, 471, 863, 1169, 1208, 1242, 1321, 1537
BstHHI	GCGC	4 cut(s)	463, 473, 1210, 1321
BstMAI	GTCTC	3 cut(s)	499, 513, 1155
BstMCI	CGRYCG	3 cut(s)	861, 1266, 1471
BstMWI	GCNNNNNNNGC	5 cut(s)	671, 812, 1064, 1076, 1124
BstNI	CCWGG	1 cut(s)	1644
BstPI	GGTNACC	2 cut(s)	1196, 1520
BstSCI	CCNGG	5 cut(s)	69, 286, 882, 1642, 1748
BstSFI	CTRYAG	2 cut(s)	77, 235
BstSNI	TACGTA	1 cut(s)	1216
BstUI	CGCG	8 cut(s)	461, 471, 863, 1169, 1208, 1242, 1321, 1537
BstV1I	GCAGC	1 cut(s)	1054
BstV2I	GAAGAC	2 cut(s)	780, 971
BstX2I	RGATCY	3 cut(s)	290, 1372, 1429
BstYI	RGATCY	3 cut(s)	290, 1372, 1429
BstZI	CGGCCG	1 cut(s)	1468
BsuRI	GGCC	4 cut(s)	119, 1139, 1470, 1642
BtgI	CCRYGG	2 cut(s)	1140, 1475
BtgZI	GCGATG	1 cut(s)	557
BtrI	CACGTC	2 cut(s)	63, 528
BtsCI	GGATG	4 cut(s)	23, 162, 1547, 1724
BtsIMutI	CAGTG	2 cut(s)	459, 564
Cac8I	GCNNGC	1 cut(s)	160
CfoI	GCGC	4 cut(s)	463, 473, 1210, 1321
Cfr10I	RCCGGY	4 cut(s)	158, 1013, 1123, 1730
Cfr13I	GGNCC	5 cut(s)	20, 29, 52, 1121, 1559
CpoI	CGGWCCG	1 cut(s)	1121
CseI	GACGC	2 cut(s)	467, 1214
Csp6I	GTAC	5 cut(s)	410, 792, 1217, 1501, 1582
CspAI	ACCGGT	2 cut(s)	1013, 1730
CspCI	CAANNNNNGTGG	2 cut(s)	542, 577
CspI	CGGWCCG	1 cut(s)	1121
CviAII	CATG	4 cut(s)	1099, 1403, 1504, 1652
CviQI	GTAC	5 cut(s)	410, 792, 1217, 1501, 1582
DdeI	CTNAG	7 cut(s)	393, 533, 1128, 1335, 1359, 1443, 1695
DraIII	CACNNNGTG	2 cut(s)	1151, 1171
DriI	GACNNNNNGTC	1 cut(s)	905
EaeI	YGGCCR	2 cut(s)	117, 1468
EagI	CGGCCG	1 cut(s)	1468
Eam1104I	CTCTTC	3 cut(s)	113, 761, 933
Eam1105I	GACNNNNNGTC	1 cut(s)	905
EarI	CTCTTC	3 cut(s)	113, 761, 933
Ecl136II	GAGCTC	1 cut(s)	316
EclXI	CGGCCG	1 cut(s)	1468
Eco105I	TACGTA	1 cut(s)	1216
Eco24I	GRGCYC	1 cut(s)	318
Eco31I	GGTCTC	1 cut(s)	1155
Eco32I	GATATC	1 cut(s)	820
Eco47I	GGWCC	5 cut(s)	20, 29, 52, 1121, 1559
Eco52I	CGGCCG	1 cut(s)	1468
Eco53kI	GAGCTC	1 cut(s)	316
Eco57I	CTGAAG	1 cut(s)	756
Eco72I	CACGTG	1 cut(s)	407
Eco88I	CYCGRG	1 cut(s)	178
Eco91I	GGTNACC	2 cut(s)	1196, 1520
EcoICRI	GAGCTC	1 cut(s)	316
EcoO109I	RGGNCCY	1 cut(s)	52
EcoO65I	GGTNACC	2 cut(s)	1196, 1520
EcoRII	CCWGG	1 cut(s)	1642
EcoRV	GATATC	1 cut(s)	820
EcoT22I	ATGCAT	1 cut(s)	266
EcoT38I	GRGCYC	1 cut(s)	318
Esp3I	CGTCTC	1 cut(s)	499
FaeI	CATG	4 cut(s)	1102, 1406, 1507, 1655
FalI	AAGNNNNNCTT	4 cut(s)	956, 988, 1194, 1226
FaqI	GGGAC	3 cut(s)	745, 1001, 1109
FatI	CATG	4 cut(s)	1098, 1402, 1503, 1651
FauI	CCCGC	1 cut(s)	1542
FblI	GTMKAC	1 cut(s)	174
Fnu4HI	GCNGC	2 cut(s)	1068, 1287
FokI	GGATG	4 cut(s)	10, 149, 1554, 1711
FriOI	GRGCYC	1 cut(s)	318
Fsp4HI	GCNGC	2 cut(s)	1068, 1287
FspBI	CTAG	3 cut(s)	1418, 1491, 1677
GlaI	GCGC	4 cut(s)	462, 472, 1209, 1320
GluI	GCNGC	2 cut(s)	1068, 1287
GsuI	CTGGAG	1 cut(s)	717
HaeIII	GGCC	4 cut(s)	119, 1139, 1470, 1642
HgaI	GACGC	2 cut(s)	467, 1214
HhaI	GCGC	4 cut(s)	463, 473, 1210, 1321
Hin1II	CATG	4 cut(s)	1102, 1406, 1507, 1655
Hin6I	GCGC	4 cut(s)	461, 471, 1208, 1319
HinP1I	GCGC	4 cut(s)	461, 471, 1208, 1319
HincII	GTYRAC	1 cut(s)	1628
HindII	GTYRAC	1 cut(s)	1628
HphI	GGTGA	4 cut(s)	661, 721, 1532, 1601
Hpy166II	GTNNAC	9 cut(s)	175, 209, 410, 457, 1177, 1325, 1501, 1628, 1686
Hpy8I	GTNNAC	9 cut(s)	175, 209, 410, 457, 1177, 1325, 1501, 1628, 1686
Hpy99I	CGWCG	6 cut(s)	308, 382, 488, 805, 903, 1462
HpyAV	CCTTC	4 cut(s)	43, 581, 601, 1750
HpyCH4III	ACNGT	9 cut(s)	414, 826, 839, 1006, 1181, 1233, 1264, 1669, 1690
HpyCH4IV	ACGT	9 cut(s)	62, 406, 527, 800, 848, 915, 1103, 1215, 1460
HpyCH4V	TGCA	4 cut(s)	264, 564, 1185, 1568
HpyF10VI	GCNNNNNNNGC	5 cut(s)	671, 812, 1064, 1076, 1124
HpyF3I	CTNAG	7 cut(s)	393, 533, 1128, 1335, 1359, 1443, 1695
HpySE526I	ACGT	9 cut(s)	62, 406, 527, 800, 848, 915, 1103, 1215, 1460
Hsp92II	CATG	4 cut(s)	1102, 1406, 1507, 1655
HspAI	GCGC	4 cut(s)	461, 471, 1208, 1319
Kpn2I	TCCGGA	2 cut(s)	26, 497
KroI	GCCGGC	1 cut(s)	158
KroNI	GCCGGC	1 cut(s)	160
LmnI	GCTCC	2 cut(s)	623, 886
Lsp1109I	GCAGC	1 cut(s)	1054
LweI	GCATC	4 cut(s)	171, 273, 387, 1141
MaeI	CTAG	3 cut(s)	1418, 1491, 1677
MaeII	ACGT	9 cut(s)	62, 406, 527, 800, 848, 915, 1103, 1215, 1460
MaeIII	GTNAC	8 cut(s)	5, 63, 438, 723, 1196, 1307, 1520, 1755
MbiI	CCGCTC	1 cut(s)	626
MflI	RGATCY	3 cut(s)	290, 1372, 1429
MhlI	GDGCHC	1 cut(s)	318
MluCI	AATT	8 cut(s)	350, 582, 655, 749, 757, 1111, 1251, 1620
MlyI	GAGTC	4 cut(s)	170, 515, 1568, 1641
MmeI	TCCRAC	6 cut(s)	112, 621, 821, 1481, 1491, 1527
MnlI	CCTC	9 cut(s)	490, 590, 739, 859, 1086, 1450, 1532, 1666, 1672
Mph1103I	ATGCAT	1 cut(s)	266
MroI	TCCGGA	2 cut(s)	26, 497
MroNI	GCCGGC	1 cut(s)	158
MseI	TTAA	5 cut(s)	296, 630, 684, 852, 918
MslI	CAYNNNNRTG	3 cut(s)	395, 516, 1733
MspR9I	CCNGG	5 cut(s)	71, 288, 884, 1644, 1750
Mva1269I	GAATGC	2 cut(s)	1250, 1288
MvaI	CCWGG	1 cut(s)	1644
MvnI	CGCG	8 cut(s)	461, 471, 863, 1169, 1208, 1242, 1321, 1537
MwoI	GCNNNNNNNGC	5 cut(s)	671, 812, 1064, 1076, 1124
NaeI	GCCGGC	1 cut(s)	160
NciI	CCSGG	4 cut(s)	71, 288, 884, 1750
NgoMIV	GCCGGC	1 cut(s)	158
NlaIII	CATG	4 cut(s)	1102, 1406, 1507, 1655
NlaIV	GGNNCC	1 cut(s)	54
NmeAIII	GCCGAG	1 cut(s)	1446
NmuCI	GTSAC	3 cut(s)	63, 1520, 1755
NsiI	ATGCAT	1 cut(s)	266
OliI	CACNNNNGTG	1 cut(s)	1733
PcsI	WCGNNNNNNNCGW	4 cut(s)	854, 912, 1212, 1394
PctI	GAATGC	2 cut(s)	1250, 1288
PdiI	GCCGGC	1 cut(s)	160
PfeI	GAWTC	9 cut(s)	103, 425, 449, 573, 808, 903, 943, 1378, 1399
PflMI	CCANNNNNTGG	1 cut(s)	1171
PfoI	TCCNGGA	2 cut(s)	69, 286
PinAI	ACCGGT	2 cut(s)	1013, 1730
PkrI	GCNGC	2 cut(s)	1069, 1288
Ple19I	CGATCG	1 cut(s)	861
PleI	GAGTC	4 cut(s)	170, 514, 1568, 1640
PmaCI	CACGTG	1 cut(s)	407
PmlI	CACGTG	1 cut(s)	407
PpsI	GAGTC	4 cut(s)	170, 514, 1568, 1640
Ppu21I	YACGTR	2 cut(s)	407, 1216
PpuMI	RGGWCCY	1 cut(s)	52
Psp124BI	GAGCTC	1 cut(s)	318
Psp1406I	AACGTT	1 cut(s)	848
Psp5II	RGGWCCY	1 cut(s)	52
Psp6I	CCWGG	1 cut(s)	1642
PspCI	CACGTG	1 cut(s)	407
PspEI	GGTNACC	2 cut(s)	1196, 1520
PspGI	CCWGG	1 cut(s)	1642
PspN4I	GGNNCC	1 cut(s)	54
PspPI	GGNCC	5 cut(s)	20, 29, 52, 1121, 1559
PspPPI	RGGWCCY	1 cut(s)	52
PsuI	RGATCY	3 cut(s)	290, 1372, 1429
PvuI	CGATCG	1 cut(s)	861
RsaI	GTAC	5 cut(s)	411, 793, 1218, 1502, 1583
RsaNI	GTAC	5 cut(s)	410, 792, 1217, 1501, 1582
RseI	CAYNNNNRTG	3 cut(s)	395, 516, 1733
Rsr2I	CGGWCCG	1 cut(s)	1121
RsrII	CGGWCCG	1 cut(s)	1121
SacI	GAGCTC	1 cut(s)	318
SaqAI	TTAA	5 cut(s)	296, 630, 684, 852, 918
SatI	GCNGC	2 cut(s)	1068, 1287
Sau96I	GGNCC	5 cut(s)	20, 29, 52, 1121, 1559
SchI	GAGTC	4 cut(s)	170, 515, 1568, 1641
ScrFI	CCNGG	5 cut(s)	71, 288, 884, 1644, 1750
SduI	GDGCHC	1 cut(s)	318
SfaNI	GCATC	4 cut(s)	171, 273, 387, 1141
SfcI	CTRYAG	2 cut(s)	77, 235
SgrAI	CRCCGGYG	1 cut(s)	1730
SinI	GGWCC	5 cut(s)	20, 29, 52, 1121, 1559
SmiMI	CAYNNNNRTG	3 cut(s)	395, 516, 1733
SmlI	CTYRAG	1 cut(s)	434
SmoI	CTYRAG	1 cut(s)	434
SnaBI	TACGTA	1 cut(s)	1216
Sse9I	AATT	8 cut(s)	350, 582, 655, 749, 757, 1111, 1251, 1620
SsiI	CCGC	9 cut(s)	300, 626, 843, 863, 1056, 1169, 1240, 1286, 1535
SspMI	CTAG	3 cut(s)	1418, 1491, 1677
SstI	GAGCTC	1 cut(s)	318
StyD4I	CCNGG	5 cut(s)	69, 286, 882, 1642, 1748
TaaI	ACNGT	9 cut(s)	414, 826, 839, 1006, 1181, 1233, 1264, 1669, 1690
TaiI	ACGT	9 cut(s)	65, 409, 530, 803, 851, 918, 1106, 1218, 1463
TaqI	TCGA	6 cut(s)	267, 377, 576, 830, 1266, 1397
TaqII	GACCGA	1 cut(s)	8
TasI	AATT	8 cut(s)	350, 582, 655, 749, 757, 1111, 1251, 1620
TatI	WGTACW	2 cut(s)	1500, 1581
TauI	GCSGC	1 cut(s)	1289
TfiI	GAWTC	9 cut(s)	103, 425, 449, 573, 808, 903, 943, 1378, 1399
Tru1I	TTAA	5 cut(s)	296, 630, 684, 852, 918
Tru9I	TTAA	5 cut(s)	296, 630, 684, 852, 918
TscAI	CASTG	2 cut(s)	459, 571
TseFI	GTSAC	3 cut(s)	63, 1520, 1755
TseI	GCWGC	1 cut(s)	1067
Tsp45I	GTSAC	3 cut(s)	63, 1520, 1755
TspDTI	ATGAA	5 cut(s)	594, 970, 1391, 1613, 1690
TspGWI	ACGGA	5 cut(s)	472, 585, 1139, 1464, 1551
TspRI	CASTG	2 cut(s)	459, 571
Van91I	CCANNNNNTGG	1 cut(s)	1171
VpaK11BI	GGWCC	5 cut(s)	20, 29, 52, 1121, 1559
XapI	RAATTY	2 cut(s)	350, 1251
XbaI	TCTAGA	1 cut(s)	1490
XmiI	GTMKAC	1 cut(s)	174
XspI	CTAG	3 cut(s)	1418, 1491, 1677
Zsp2I	ATGCAT	1 cut(s)	266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.