Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007564

Physical Location & Seq

Genomic Coordinates Forward (+)

31274274 .. 31279124

Gene Structure 4851 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1220700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGGCTACGGCAGACTCCAGCCGTCTGACCCTGGAGGCTCCCCCAGTCTCTACGTGGCGGAAAACCCATCACTCCCACAAACTGCCACGTCATCCTCCTGCTCCAACGGCCGCAGCCGCAAGAACAAGCTCATCCTAATGTTCCTCCTCGCCGTGGCACTCATCCTCGCCTCCGCCGTCTCGGCGGTGGTACTCATCGTCGGAAAATCCAAAGCCTCAAACCCCGCCGCCTCCTCCGCCCTCCGCCGCAAGCCAACGCAGGCTATCTCAAACGCGTGCGCAAAGACGCGCTTCCCCTCCCTCTGCGTCGACTCCCTCCTCGACTTCCCCGGCTCCAACGCCGCGTCGGAGCAGGACCTCGTCCACATTTCCTTCAACATGACTCTGCAGCGACTCAGCAAGGCGCTCTACCTCTCCTCCTCCCTCTCCTACCGCCAGATGGACCCCCACTCCCGCTCCGCCTATGACGACTGCCTCGAGCTCCTCGACGACTCCGTAGACGCCCTCTCCCGGGCCCTCACCTCCGTCGCCCCCGGCGTGGAGTCCTCGTCCACACAAGATGTCCGGACGTGGCTGAGCGCGGCGCTCACGAACCAGGACACGTGCGGGGAGGGTTTTGCGCAAATCAAGGGCGGCGACGTCAAGAACGAGATGGACCAGCGGCTCAAGGACTTGTCAGAACTGGTGAGCAACTGCCTCGCGATTTACTCGGCGGTCGCAGGCGACGATTTCTCCGGGGTGCCGATACAGAACAAGCGGAGGAGGTTGTTGGGGAATTCAGAAATGGAGCGTGATATAATGGGGGATAATGCTGACGTCTCGCGAGATTTTCCGAAGTGGTTAGGAAGGAGGGAGAGGAGGCTGCTGTCGGTGCCGGTATGGCAGATACAGGCGGATATTATCGTGTCAAAAGACGGCAATGGGACGTACAAGACGATCGCCGAGGCGATCAAAAAGGCGCCGGAGTATAGTTCTCGTCGGACAATTATTTACGTCAGGGCAGGAAGGTACGAAGAGAAGAATTTGAAGGTGGGGAGGAAGAAGACCAACTTGATGTTCGTCGGAGACGGGAAGGGCAAAACCGTAATCACCGGCGGGAAAAGCGTCTCTCAGAACCTGACCACATTCCACACTGCATCCTTCGCTGCCACCGGAGCTGGTTTTATTGCACGTGACATAACGTTCGAGAACTACGCCGGACCGGCCAAGCACCAAGCCGTTGCCCTTCGAATTGGGGCGGACCATGCCGTGGTCTACCTTTGCAACATCATCGGATACCAAGACACATTTTACGTGCACTCGAATCGTCAATTCATCCGTGAAACGGACATTTACGGTACAGTGGACTTCATTTTTGGCAATGCGGCCGTAGTGTTCCAAAACTGTAGCATCTACGCTCGCAAGCCCATGGCCTTCCAAAAAAACACAATCACTGCCCAAAATCGAAAGGACCCGAATCAGAACACGGGCATTTCAATCCATGCTTGCCGGATTCTCGCCACTCCAGAGCTCGAGGGATCAAAGGGTAACTTCTCAACGTATCTAGGCCGTCCGTGGAAGCTGTACTCTAGGGCAGTGTACATGTTATCGTTCATTGGTGATCACGTACATCCACATGGATGGCTTGAGTGGAATACCACTTTTGCTCTCGATACGCTATATTATGGTGAATACATGAACTATGGCCCCGGCGGAGCGATAGGTAAACGGGTAACTTGGCCGGGGTATCGCGTCATTACATCCCAGGTGGAGGCGGCTAGGTTCACCGTGCGACAGTTTATATACGGTTCGTCGTGGTTGCCATCCACCGGGGTGGCATTCTTGGCCGATCTATCACTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0010119 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050789 GO:0050790 GO:0050794 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

614

Amino Acids

67.27

Weight (kDa)

9.24

Isoelectric Point (pI)

35.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	88 - 235	1.6e-37	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	299 - 596	1.8e-139	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49220 AT3G49220 AT3G49220 AT5G53370 AT5G53370
fragaria_vesca	FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650
malus_domestica	MD01G1220700.v1.1 MD07G1289200.v1.1
prunus_persica	Prupe.2G310600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0380731
rosa_laevigata	RLG00000026273
rosa_multiflora	Rmu_sc0001637.1_g000012
rosa_roxburghii	Rroxscaffold_4G00279070
rosa_rugosa	Rorug01G0424000
rosa_samantha	Rh1AG448200 Rh1BG403600 Rh1CG417900 Rh1DG433900
rosa_wichuraiana	Rw0G005890 Rw1G039010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	642, 819
Acc16I	TGCGCA	2 cut(s)	280, 621
AccB1I	GGYRCC	3 cut(s)	739, 871, 958
AccB7I	CCANNNNNTGG	1 cut(s)	1249
AccBSI	CCGCTC	1 cut(s)	456
AccI	GTMKAC	3 cut(s)	309, 498, 1254
AccII	CGCG	7 cut(s)	275, 289, 344, 581, 701, 823, 1731
AccIII	TCCGGA	1 cut(s)	564
AclI	AACGTT	1 cut(s)	1181
AclWI	GGATC	1 cut(s)	1525
AcoI	YGGCCR	5 cut(s)	109, 1203, 1365, 1718, 1824
AcsI	RAATTY	2 cut(s)	775, 1021
AcvI	CACGTG	2 cut(s)	603, 1172
AcyI	GRCGYC	4 cut(s)	501, 639, 816, 959
AfaI	GTAC	7 cut(s)	192, 929, 1010, 1339, 1565, 1580, 1608
AfiI	CCNNNNNNNGG	9 cut(s)	154, 439, 510, 511, 538, 1249, 1324, 1750, 1808
AflIII	ACRYGT	3 cut(s)	273, 600, 1581
AgsI	TTSAA	3 cut(s)	376, 1027, 1476
AjiI	CACGTC	2 cut(s)	90, 570
AjnI	CCWGG	3 cut(s)	31, 594, 1743
AjuI	GAANNNNNNNTTGG	2 cut(s)	1040, 1072
AleI	CACNNNNGTG	1 cut(s)	1811
AluBI	AGCT	5 cut(s)	130, 481, 1157, 1510, 1561
AluI	AGCT	5 cut(s)	130, 481, 1157, 1510, 1561
Alw21I	GWGCWC	3 cut(s)	483, 1299, 1512
Alw26I	GTCTC	5 cut(s)	53, 184, 823, 1059, 1111
Alw44I	GTGCAC	1 cut(s)	1295
AlwI	GGATC	1 cut(s)	1525
AlwNI	CAGNNNCTG	1 cut(s)	1117
Ama87I	CYCGRG	3 cut(s)	476, 510, 1511
Aor13HI	TCCGGA	1 cut(s)	564
AoxI	GGCC	9 cut(s)	109, 513, 1203, 1365, 1410, 1546, 1684, 1718, 1824
ApaI	GGGCCC	1 cut(s)	517
ApaLI	GTGCAC	1 cut(s)	1295
ApeKI	GCWGC	4 cut(s)	114, 388, 862, 1145
ApoI	RAATTY	2 cut(s)	775, 1021
ArsI	GACNNNNNNTTYG	4 cut(s)	1166, 1198, 1337, 1369
AspLEI	GCGC	7 cut(s)	281, 291, 406, 581, 586, 622, 961
AspS9I	GGNCC	9 cut(s)	355, 442, 513, 514, 655, 1199, 1240, 1450, 1685
AsuC2I	CCSGG	8 cut(s)	330, 511, 512, 534, 736, 1689, 1722, 1810
AsuHPI	GGTGA	6 cut(s)	511, 697, 1081, 1610, 1679, 1756
AsuII	TTCGAA	1 cut(s)	1228
AvaI	CYCGRG	3 cut(s)	476, 510, 1511
AvaII	GGWCC	6 cut(s)	355, 442, 655, 1199, 1240, 1450
BaeGI	GKGCMC	2 cut(s)	517, 1299
BanI	GGYRCC	3 cut(s)	739, 871, 958
BanII	GRGCYC	3 cut(s)	483, 517, 1512
BbrPI	CACGTG	2 cut(s)	603, 1172
BbsI	GAAGAC	1 cut(s)	1049
Bbv12I	GWGCWC	3 cut(s)	483, 1299, 1512
BbvI	GCAGC	4 cut(s)	126, 400, 849, 1132
BccI	CCATC	5 cut(s)	76, 433, 646, 1614, 1810
BcgI	CGANNNNNNTGC	6 cut(s)	679, 713, 1252, 1286, 1286, 1320
BciT130I	CCWGG	3 cut(s)	33, 596, 1745
BciVI	GTATCC	1 cut(s)	1268
BclI	TGATCA	1 cut(s)	1600
BcnI	CCSGG	8 cut(s)	330, 511, 512, 534, 736, 1689, 1722, 1810
BcoDI	GTCTC	5 cut(s)	53, 184, 823, 1059, 1111
BfaI	CTAG	3 cut(s)	1544, 1569, 1758
BfmI	CTRYAG	2 cut(s)	386, 1384
BfoI	RGCGCY	3 cut(s)	407, 587, 962
BfuI	GTATCC	1 cut(s)	1268
BglI	GCCNNNNNGGC	3 cut(s)	182, 880, 1202
BlpI	GCTNAGC	1 cut(s)	575
Bme18I	GGWCC	6 cut(s)	355, 442, 655, 1199, 1240, 1450
BmeT110I	CYCGRG	3 cut(s)	476, 510, 1511
BmgBI	CACGTC	2 cut(s)	90, 570
BmgT120I	GGNCC	9 cut(s)	355, 442, 513, 514, 655, 1199, 1240, 1450, 1685
BmiI	GGNNCC	9 cut(s)	40, 334, 444, 515, 741, 873, 960, 1452, 1687
BmrI	ACTGGG	1 cut(s)	39
BmsI	GCATC	2 cut(s)	1145, 1398
BmuI	ACTGGG	1 cut(s)	39
BpiI	GAAGAC	1 cut(s)	1049
BpmI	CTGGAG	2 cut(s)	54, 1488
Bpu1102I	GCTNAGC	1 cut(s)	575
Bpu14I	TTCGAA	1 cut(s)	1228
BpuEI	CTTGAG	2 cut(s)	650, 1646
BpuMI	CCSGG	8 cut(s)	330, 511, 512, 534, 736, 1689, 1722, 1810
BsaAI	YACGTR	5 cut(s)	55, 603, 1172, 1294, 1606
BsaHI	GRCGYC	4 cut(s)	501, 639, 816, 959
BsaWI	WCCGGW	2 cut(s)	564, 1151
Bsc4I	CCNNNNNNNGG	9 cut(s)	154, 439, 510, 511, 538, 1249, 1324, 1750, 1808
Bse118I	RCCGGY	3 cut(s)	874, 1091, 1201
Bse1I	ACTGG	2 cut(s)	45, 687
Bse3DI	GCAATG	2 cut(s)	925, 1366
BseAI	TCCGGA	1 cut(s)	564
BseBI	CCWGG	3 cut(s)	33, 596, 1745
BseGI	GGATG	9 cut(s)	92, 132, 162, 1136, 1314, 1609, 1625, 1739, 1802
BseLI	CCNNNNNNNGG	9 cut(s)	154, 439, 510, 511, 538, 1249, 1324, 1750, 1808
BseMI	GCAATG	2 cut(s)	925, 1366
BseMII	CTCAG	3 cut(s)	409, 566, 1124
BseNI	ACTGG	2 cut(s)	45, 687
BseRI	GAGGAG	8 cut(s)	137, 223, 308, 406, 409, 473, 775, 871
BseSI	GKGCMC	2 cut(s)	517, 1299
BseX3I	CGGCCG	2 cut(s)	109, 1365
BseXI	GCAGC	4 cut(s)	126, 400, 849, 1132
Bsh1236I	CGCG	7 cut(s)	275, 289, 344, 581, 701, 823, 1731
Bsh1285I	CGRYCG	4 cut(s)	112, 717, 939, 1368
BshFI	GGCC	9 cut(s)	111, 515, 1205, 1367, 1412, 1548, 1686, 1720, 1826
BshNI	GGYRCC	3 cut(s)	739, 871, 958
BsiEI	CGRYCG	4 cut(s)	112, 717, 939, 1368
BsiHKAI	GWGCWC	3 cut(s)	483, 1299, 1512
BsiHKCI	CYCGRG	3 cut(s)	476, 510, 1511
BslFI	GGGAC	1 cut(s)	937
BslI	CCNNNNNNNGG	9 cut(s)	154, 439, 510, 511, 538, 1249, 1324, 1750, 1808
BsmAI	GTCTC	5 cut(s)	53, 184, 823, 1059, 1111
BsmBI	CGTCTC	4 cut(s)	184, 823, 1059, 1111
BsmFI	GGGAC	1 cut(s)	937
BsmI	GAATGC	1 cut(s)	1817
BsnI	GGCC	9 cut(s)	111, 515, 1205, 1367, 1412, 1548, 1686, 1720, 1826
BsoBI	CYCGRG	3 cut(s)	476, 510, 1511
Bsp119I	TTCGAA	1 cut(s)	1228
Bsp120I	GGGCCC	1 cut(s)	513
Bsp1286I	GDGCHC	4 cut(s)	483, 517, 1299, 1512
Bsp13I	TCCGGA	1 cut(s)	564
Bsp1407I	TGTACA	1 cut(s)	1578
Bsp143I	GATC	5 cut(s)	936, 948, 1517, 1600, 1828
Bsp1720I	GCTNAGC	1 cut(s)	575
Bsp19I	CCATGG	1 cut(s)	1407
Bsp68I	TCGCGA	2 cut(s)	701, 823
BspANI	GGCC	9 cut(s)	111, 515, 1205, 1367, 1412, 1548, 1686, 1720, 1826
BspCNI	CTCAG	3 cut(s)	408, 567, 1123
BspEI	TCCGGA	1 cut(s)	564
BspFNI	CGCG	7 cut(s)	275, 289, 344, 581, 701, 823, 1731
BspLI	GGNNCC	9 cut(s)	40, 334, 444, 515, 741, 873, 960, 1452, 1687
BspMAI	CTGCAG	1 cut(s)	390
BspPI	GGATC	1 cut(s)	1525
BspT104I	TTCGAA	1 cut(s)	1228
BspT107I	GGYRCC	3 cut(s)	739, 871, 958
BsrBI	CCGCTC	1 cut(s)	456
BsrDI	GCAATG	2 cut(s)	925, 1366
BsrFI	RCCGGY	3 cut(s)	874, 1091, 1201
BsrGI	TGTACA	1 cut(s)	1578
BsrI	ACTGG	2 cut(s)	45, 687
BssAI	RCCGGY	3 cut(s)	874, 1091, 1201
BssMI	GATC	5 cut(s)	936, 948, 1517, 1600, 1828
BssNI	GRCGYC	4 cut(s)	501, 639, 816, 959
BssT1I	CCWWGG	1 cut(s)	1407
Bst2UI	CCWGG	3 cut(s)	33, 596, 1745
Bst4CI	ACNGT	7 cut(s)	1084, 1337, 1342, 1385, 1768, 1776, 1787
Bst6I	CTCTTC	1 cut(s)	1008
BstACI	GRCGYC	4 cut(s)	501, 639, 816, 959
BstAUI	TGTACA	1 cut(s)	1578
BstBAI	YACGTR	5 cut(s)	55, 603, 1172, 1294, 1606
BstBI	TTCGAA	1 cut(s)	1228
BstC8I	GCNNGC	7 cut(s)	251, 261, 277, 721, 1399, 1403, 1486
BstDEI	CTNAG	3 cut(s)	395, 575, 1110
BstDSI	CCRYGG	4 cut(s)	153, 1248, 1407, 1553
BstF5I	GGATG	9 cut(s)	92, 132, 162, 1136, 1314, 1609, 1625, 1739, 1802
BstFNI	CGCG	7 cut(s)	275, 289, 344, 581, 701, 823, 1731
BstH2I	RGCGCY	3 cut(s)	407, 587, 962
BstHHI	GCGC	7 cut(s)	281, 291, 406, 581, 586, 622, 961
BstKTI	GATC	5 cut(s)	939, 951, 1520, 1603, 1831
BstMAI	GTCTC	5 cut(s)	53, 184, 823, 1059, 1111
BstMBI	GATC	5 cut(s)	936, 948, 1517, 1600, 1828
BstMCI	CGRYCG	4 cut(s)	112, 717, 939, 1368
BstNI	CCWGG	3 cut(s)	33, 596, 1745
BstNSI	RCATGY	1 cut(s)	1585
BstSFI	CTRYAG	2 cut(s)	386, 1384
BstSLI	GKGCMC	2 cut(s)	517, 1299
BstUI	CGCG	7 cut(s)	275, 289, 344, 581, 701, 823, 1731
BstV1I	GCAGC	4 cut(s)	126, 400, 849, 1132
BstV2I	GAAGAC	1 cut(s)	1049
BstXI	CCANNNNNNTGG	2 cut(s)	1620, 1813
BstZI	CGGCCG	2 cut(s)	109, 1365
BsuI	GTATCC	1 cut(s)	1268
BsuRI	GGCC	9 cut(s)	111, 515, 1205, 1367, 1412, 1548, 1686, 1720, 1826
BtgI	CCRYGG	4 cut(s)	153, 1248, 1407, 1553
BtrI	CACGTC	2 cut(s)	90, 570
BtsCI	GGATG	9 cut(s)	92, 132, 162, 1136, 1314, 1609, 1625, 1739, 1802
BtsI	GCAGTG	3 cut(s)	1131, 1431, 1581
BtsIMutI	CAGTG	4 cut(s)	1131, 1347, 1431, 1581
BtuMI	TCGCGA	2 cut(s)	701, 823
Cac8I	GCNNGC	7 cut(s)	251, 261, 277, 721, 1399, 1403, 1486
CaiI	CAGNNNCTG	1 cut(s)	1117
CfoI	GCGC	7 cut(s)	281, 291, 406, 581, 586, 622, 961
Cfr10I	RCCGGY	3 cut(s)	874, 1091, 1201
Cfr13I	GGNCC	9 cut(s)	355, 442, 513, 514, 655, 1199, 1240, 1450, 1685
Cfr9I	CCCGGG	1 cut(s)	510
CpoI	CGGWCCG	1 cut(s)	1199
CseI	GACGC	6 cut(s)	295, 295, 333, 509, 1093, 1720
Csp6I	GTAC	7 cut(s)	191, 928, 1009, 1338, 1564, 1579, 1607
CspI	CGGWCCG	1 cut(s)	1199
CviAII	CATG	7 cut(s)	379, 1244, 1408, 1481, 1582, 1616, 1675
CviQI	GTAC	7 cut(s)	191, 928, 1009, 1338, 1564, 1579, 1607
DdeI	CTNAG	3 cut(s)	395, 575, 1110
DinI	GGCGCC	1 cut(s)	960
DpnI	GATC	5 cut(s)	938, 950, 1519, 1602, 1830
DpnII	GATC	5 cut(s)	936, 948, 1517, 1600, 1828
EaeI	YGGCCR	5 cut(s)	109, 1203, 1365, 1718, 1824
EagI	CGGCCG	2 cut(s)	109, 1365
Eam1104I	CTCTTC	1 cut(s)	1008
EarI	CTCTTC	1 cut(s)	1008
EciI	GGCGGA	8 cut(s)	74, 163, 226, 233, 448, 908, 1253, 1707
Ecl136II	GAGCTC	2 cut(s)	481, 1510
EclXI	CGGCCG	2 cut(s)	109, 1365
Eco130I	CCWWGG	1 cut(s)	1407
Eco24I	GRGCYC	3 cut(s)	483, 517, 1512
Eco47I	GGWCC	6 cut(s)	355, 442, 655, 1199, 1240, 1450
Eco52I	CGGCCG	2 cut(s)	109, 1365
Eco53kI	GAGCTC	2 cut(s)	481, 1510
Eco72I	CACGTG	2 cut(s)	603, 1172
Eco88I	CYCGRG	3 cut(s)	476, 510, 1511
EcoICRI	GAGCTC	2 cut(s)	481, 1510
EcoO109I	RGGNCCY	3 cut(s)	355, 514, 1450
EcoRI	GAATTC	1 cut(s)	775
EcoRII	CCWGG	3 cut(s)	31, 594, 1743
EcoT14I	CCWWGG	1 cut(s)	1407
EcoT38I	GRGCYC	3 cut(s)	483, 517, 1512
EgeI	GGCGCC	1 cut(s)	960
EheI	GGCGCC	1 cut(s)	960
ErhI	CCWWGG	1 cut(s)	1407
Esp3I	CGTCTC	4 cut(s)	184, 823, 1059, 1111
FaeI	CATG	7 cut(s)	382, 1247, 1411, 1484, 1585, 1619, 1678
FalI	AAGNNNNNCTT	6 cut(s)	275, 307, 1034, 1066, 1514, 1546
FaqI	GGGAC	1 cut(s)	937
FatI	CATG	7 cut(s)	378, 1243, 1407, 1480, 1581, 1615, 1674
FauI	CCCGC	4 cut(s)	232, 461, 599, 1088
FbaI	TGATCA	1 cut(s)	1600
FblI	GTMKAC	3 cut(s)	309, 498, 1254
FokI	GGATG	9 cut(s)	79, 119, 149, 1123, 1301, 1596, 1632, 1726, 1789
FriOI	GRGCYC	3 cut(s)	483, 517, 1512
FspBI	CTAG	3 cut(s)	1544, 1569, 1758
FspI	TGCGCA	2 cut(s)	280, 621
GlaI	GCGC	7 cut(s)	280, 290, 405, 580, 585, 621, 960
GsuI	CTGGAG	2 cut(s)	54, 1488
HaeII	RGCGCY	3 cut(s)	407, 587, 962
HaeIII	GGCC	9 cut(s)	111, 515, 1205, 1367, 1412, 1548, 1686, 1720, 1826
HgaI	GACGC	6 cut(s)	295, 295, 333, 509, 1093, 1720
HhaI	GCGC	7 cut(s)	281, 291, 406, 581, 586, 622, 961
Hin1I	GRCGYC	4 cut(s)	501, 639, 816, 959
Hin1II	CATG	7 cut(s)	382, 1247, 1411, 1484, 1585, 1619, 1678
Hin6I	GCGC	7 cut(s)	279, 289, 404, 579, 584, 620, 959
HinP1I	GCGC	7 cut(s)	279, 289, 404, 579, 584, 620, 959
HincII	GTYRAC	1 cut(s)	310
HindII	GTYRAC	1 cut(s)	310
HinfI	GANTC	9 cut(s)	15, 311, 382, 393, 491, 542, 1303, 1456, 1492
HphI	GGTGA	6 cut(s)	511, 697, 1081, 1610, 1679, 1756
Hpy188III	TCNNGA	8 cut(s)	565, 589, 643, 700, 822, 1186, 1505, 1649
HpyAV	CCTTC	8 cut(s)	382, 840, 999, 1021, 1066, 1150, 1235, 1423
HpyCH4III	ACNGT	7 cut(s)	1084, 1337, 1342, 1385, 1768, 1776, 1787
HpyCH4V	TGCA	5 cut(s)	388, 1136, 1169, 1263, 1297
HpyF3I	CTNAG	3 cut(s)	395, 575, 1110
Hsp92I	GRCGYC	4 cut(s)	501, 639, 816, 959
Hsp92II	CATG	7 cut(s)	382, 1247, 1411, 1484, 1585, 1619, 1678
HspAI	GCGC	7 cut(s)	279, 289, 404, 579, 584, 620, 959
KasI	GGCGCC	1 cut(s)	958
Kpn2I	TCCGGA	1 cut(s)	564
Ksp22I	TGATCA	1 cut(s)	1600
Kzo9I	GATC	5 cut(s)	936, 948, 1517, 1600, 1828
LmnI	GCTCC	9 cut(s)	44, 107, 338, 349, 461, 486, 787, 1154, 1694
Lsp1109I	GCAGC	4 cut(s)	126, 400, 849, 1132
LweI	GCATC	2 cut(s)	1145, 1398
MaeI	CTAG	3 cut(s)	1544, 1569, 1758
MaeIII	GTNAC	3 cut(s)	1172, 1526, 1711
MalI	GATC	5 cut(s)	938, 950, 1519, 1602, 1830
MbiI	CCGCTC	1 cut(s)	456
MboI	GATC	5 cut(s)	936, 948, 1517, 1600, 1828
MboII	GAAGA	4 cut(s)	1025, 1030, 1051, 1054
MhlI	GDGCHC	4 cut(s)	483, 517, 1299, 1512
MluCI	AATT	5 cut(s)	775, 984, 1021, 1230, 1310
MluI	ACGCGT	1 cut(s)	273
Mly113I	GGCGCC	1 cut(s)	959
MlyI	GAGTC	6 cut(s)	9, 305, 376, 387, 485, 551
MmeI	TCCRAC	6 cut(s)	129, 181, 327, 360, 959, 1042
MroI	TCCGGA	1 cut(s)	564
MslI	CAYNNNNRTG	4 cut(s)	137, 1614, 1618, 1811
MspA1I	CMGCKG	1 cut(s)	661
MteI	GCGCNGCGC	1 cut(s)	582
Mva1269I	GAATGC	1 cut(s)	1817
MvaI	CCWGG	3 cut(s)	33, 596, 1745
MvnI	CGCG	7 cut(s)	275, 289, 344, 581, 701, 823, 1731
NarI	GGCGCC	1 cut(s)	959
NciI	CCSGG	8 cut(s)	330, 511, 512, 534, 736, 1689, 1722, 1810
NcoI	CCATGG	1 cut(s)	1407
NdeII	GATC	5 cut(s)	936, 948, 1517, 1600, 1828
NlaIII	CATG	7 cut(s)	382, 1247, 1411, 1484, 1585, 1619, 1678
NlaIV	GGNNCC	9 cut(s)	40, 334, 444, 515, 741, 873, 960, 1452, 1687
NmeAIII	GCCGAG	3 cut(s)	161, 689, 967
NmuCI	GTSAC	1 cut(s)	1172
NruI	TCGCGA	2 cut(s)	701, 823
NsbI	TGCGCA	2 cut(s)	280, 621
NspI	RCATGY	1 cut(s)	1585
NspV	TTCGAA	1 cut(s)	1228
OliI	CACNNNNGTG	1 cut(s)	1811
PaeR7I	CTCGAG	2 cut(s)	476, 1511
PciI	ACATGT	1 cut(s)	1581
PcsI	WCGNNNNNNNCGW	8 cut(s)	174, 474, 483, 492, 534, 645, 723, 932
PctI	GAATGC	1 cut(s)	1817
PfeI	GAWTC	3 cut(s)	1303, 1456, 1492
PflFI	GACNNNGTC	1 cut(s)	359
PflMI	CCANNNNNTGG	1 cut(s)	1249
Ple19I	CGATCG	1 cut(s)	939
PleI	GAGTC	6 cut(s)	9, 305, 376, 387, 485, 550
PluTI	GGCGCC	1 cut(s)	962
PmaCI	CACGTG	2 cut(s)	603, 1172
PmlI	CACGTG	2 cut(s)	603, 1172
PpsI	GAGTC	6 cut(s)	9, 305, 376, 387, 485, 550
Ppu21I	YACGTR	5 cut(s)	55, 603, 1172, 1294, 1606
PpuMI	RGGWCCY	2 cut(s)	355, 1450
PscI	ACATGT	1 cut(s)	1581
Psp124BI	GAGCTC	2 cut(s)	483, 1512
Psp1406I	AACGTT	1 cut(s)	1181
Psp5II	RGGWCCY	2 cut(s)	355, 1450
Psp6I	CCWGG	3 cut(s)	31, 594, 1743
PspCI	CACGTG	2 cut(s)	603, 1172
PspGI	CCWGG	3 cut(s)	31, 594, 1743
PspN4I	GGNNCC	9 cut(s)	40, 334, 444, 515, 741, 873, 960, 1452, 1687
PspOMI	GGGCCC	1 cut(s)	513
PspPI	GGNCC	9 cut(s)	355, 442, 513, 514, 655, 1199, 1240, 1450, 1685
PspPPI	RGGWCCY	2 cut(s)	355, 1450
PspXI	VCTCGAGB	2 cut(s)	476, 1511
PstI	CTGCAG	1 cut(s)	390
PstNI	CAGNNNCTG	1 cut(s)	1117
PsyI	GACNNNGTC	1 cut(s)	359
PvuI	CGATCG	1 cut(s)	939
RruI	TCGCGA	2 cut(s)	701, 823
RsaI	GTAC	7 cut(s)	192, 929, 1010, 1339, 1565, 1580, 1608
RsaNI	GTAC	7 cut(s)	191, 928, 1009, 1338, 1564, 1579, 1607
RseI	CAYNNNNRTG	4 cut(s)	137, 1614, 1618, 1811
Rsr2I	CGGWCCG	1 cut(s)	1199
RsrII	CGGWCCG	1 cut(s)	1199
SacI	GAGCTC	2 cut(s)	483, 1512
SalI	GTCGAC	1 cut(s)	308
Sau3AI	GATC	5 cut(s)	936, 948, 1517, 1600, 1828
Sau96I	GGNCC	9 cut(s)	355, 442, 513, 514, 655, 1199, 1240, 1450, 1685
SchI	GAGTC	6 cut(s)	9, 305, 376, 387, 485, 551
SduI	GDGCHC	4 cut(s)	483, 517, 1299, 1512
SfaNI	GCATC	2 cut(s)	1145, 1398
SfcI	CTRYAG	2 cut(s)	386, 1384
SfoI	GGCGCC	1 cut(s)	960
Sfr274I	CTCGAG	2 cut(s)	476, 1511
SfuI	TTCGAA	1 cut(s)	1228
SgrAI	CRCCGGYG	1 cut(s)	1091
SinI	GGWCC	6 cut(s)	355, 442, 655, 1199, 1240, 1450
SlaI	CTCGAG	2 cut(s)	476, 1511
SmaI	CCCGGG	1 cut(s)	512
SmiMI	CAYNNNNRTG	4 cut(s)	137, 1614, 1618, 1811
SmlI	CTYRAG	4 cut(s)	476, 665, 1511, 1625
SmoI	CTYRAG	4 cut(s)	476, 665, 1511, 1625
Sse9I	AATT	5 cut(s)	775, 984, 1021, 1230, 1310
SspDI	GGCGCC	1 cut(s)	958
SspMI	CTAG	3 cut(s)	1544, 1569, 1758
SstI	GAGCTC	2 cut(s)	483, 1512
StyI	CCWWGG	1 cut(s)	1407
TaaI	ACNGT	7 cut(s)	1084, 1337, 1342, 1385, 1768, 1776, 1787
TasI	AATT	5 cut(s)	775, 984, 1021, 1230, 1310
TatI	WGTACW	2 cut(s)	1563, 1578
TfiI	GAWTC	3 cut(s)	1303, 1456, 1492
TscAI	CASTG	4 cut(s)	1138, 1347, 1438, 1581
TseFI	GTSAC	1 cut(s)	1172
TseI	GCWGC	4 cut(s)	114, 388, 862, 1145
Tsp45I	GTSAC	1 cut(s)	1172
TspDTI	ATGAA	4 cut(s)	1303, 1339, 1582, 1691
TspGWI	ACGGA	5 cut(s)	484, 514, 1307, 1340, 1542
TspMI	CCCGGG	1 cut(s)	510
TspRI	CASTG	4 cut(s)	1138, 1347, 1438, 1581
Tth111I	GACNNNGTC	1 cut(s)	359
Van91I	CCANNNNNTGG	1 cut(s)	1249
VneI	GTGCAC	1 cut(s)	1295
VpaK11BI	GGWCC	6 cut(s)	355, 442, 655, 1199, 1240, 1450
XapI	RAATTY	2 cut(s)	775, 1021
XceI	RCATGY	1 cut(s)	1585
XcmI	CCANNNNNNNNNTGG	1 cut(s)	52
XhoI	CTCGAG	2 cut(s)	476, 1511
XmaI	CCCGGG	1 cut(s)	510
XmiI	GTMKAC	3 cut(s)	309, 498, 1254
XspI	CTAG	3 cut(s)	1544, 1569, 1758
ZraI	GACGTC	2 cut(s)	640, 817

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.