Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0007564

Physical Location & Seq

Genomic Coordinates Reverse (-)

52679582 .. 52683972

Gene Structure 4391 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG403600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGGGATACGGCCGTCTCCAACCCTCCGACCCCGGAGGCTCCTCCCGCTCCATGGGAGACCAATCCCCGCCGCAACAACCACCGTCTTGTAAAAGCAAGAAAAAGCTCATCATACTCTCCATTCTCTCCATCACTCTCATCGCCGCAGCCGCCGTCTCCGCAGTCCTCCTCATCGCAGTCCGATCCAAGCCGGCCTCGAAGCAGTCCCTCCTCCACCGCCAGCCGACGCAAGCCATCTCTGACGCCTGCTCCAAGACTCGCTTCCCTTCTCTCTGCGTCAACTCCCTCCTCGACTTCCCGGGATCTCTGAACGCGTCGGAGGCCGACCTCGTCCACATTTCCTTCAACATGACGCTGCAGCACCTCAGCAAAGCCCTCTACCTCTCCTCGGGGCTATCGTATCTCCAGATGGACCCACACGCCAAGTCCGCCTACCAGGACTGCCTCGAGCTCCTGGACGACTCTGTGGACGCGCTCACGCGCGCCCTGACTACCGTCGCGCCTGGACTCGACGGTAGCCCCGGGTCTAGCGAGGACGTGCTGACCTGGCTGAGCTCGGCGCTGACAAATCAGGACACGTGCGGGGAGGGCCTCGCCGACGTGAGCGGCCCGGTGAAAGAGGAGCTGGGCCAGAAGCTCAAGGACCTGTCGGAGCTGGTGAGTAACTGCCTGGCGATATTTACAGCGACTAGTAGTAACGACTTCTCGGGAGTGCCGATTCAGAACAGGAGATTGCTGATGGAGAGTGATATAATGGGAGGAGATAATGCCGATGTGTCGCGGGATTTCCCGAGATGGTTGGGTAAGAAGGAGAGGATGTTGCTGGCTTTGCCGGTGGCACAGATACAGGCCGATATAATCGTCTCCAAGGACGGAAATGGGACCTACAAGACGATCGCCGAGGCGATCAAAAAGGCGCCGGAGTATAGTAAACGCCGGACAGTTATTTACGTCAGGGCAGGAAGGTATGAAGAAGATAATCTGAAGGTAGGAAGGAAGAAAACGAACTTGATGTTCATCGGCGACGGCAAGGGTATAACCATAATCAGCGGTGGTAAAAGCGTTTCCGAAAAGCTAACCACATTCCGCACCGCATCTTTCGCGGCTACCGGAGCAGGCTTCATTGCACGGGACATAACGTTTGAGAACTATGCCGGCCCGCAGAAGCACCAAGCTGTCGCCCTCCGCGTTGGGGCAGATCATGCCGTGGTCTACCGGTGCAACATTATCGGATACCAAGACACGTTTTATGTGCACTCGAACAGACAGTTCGTTCGGGAATGTGACATTTATGGTACTGTGGACTTCATATTTGGCAATGCAGCCGTGGTGTTTCAAAATTGTAGTATCTACGCCCGCAAGCCAATGCCCTCTCAGAAAAACACCATCACGGCTCAAAACAGGAAAGACCCGAATCAAAACACGGGGATGTCGATCCACGCTTGCCGGATCCTTGCCGCGTCGGATCTTGCCCCCGTAAAGGGTAGCTTTCCGACGTATTTGGGTCGCCCTTGGAAAATGTACTCTAGGGTTGTGTACATGTTATCATACATTGGGGATCATGTAGCTCCCCGAGGATGGCTCGAGTGGAATTCCAATTTTGCTCTAGATACTCTCTACTACGGTGAATACATGAACTATGGGCCGGGCGGGGCTGTAGGTCAACGTGTAAAATGGCCAGGGTATCGGGTAATTACGTCCACCGTTGAGGCCAACAAGTTCACCGTTGCACAGTTTATATATGGCTCATCATGGTTACCCTCCACCGGAGTGGCATTCTTGGGTGGCCTTTCGGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0010119 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050789 GO:0050790 GO:0050794 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

599

Amino Acids

65.2

Weight (kDa)

8.99

Isoelectric Point (pI)

40.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	78 - 226	4.9e-35	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	285 - 582	4.2e-144	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49220 AT3G49220 AT3G49220 AT5G53370 AT5G53370
fragaria_vesca	FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650
malus_domestica	MD01G1220700.v1.1 MD07G1289200.v1.1
prunus_persica	Prupe.2G310600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0380731
rosa_laevigata	RLG00000026273
rosa_multiflora	Rmu_sc0001637.1_g000012
rosa_roxburghii	Rroxscaffold_4G00279070
rosa_rugosa	Rorug01G0424000
rosa_samantha	Rh1AG448200 Rh1BG403600 Rh1CG417900 Rh1DG433900
rosa_wichuraiana	Rw0G005890 Rw1G039010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	916
AccBSI	CCGCTC	2 cut(s)	48, 606
AccI	GTMKAC	1 cut(s)	1212
AccII	CGCG	9 cut(s)	314, 473, 481, 483, 500, 781, 1103, 1188, 1460
AclI	AACGTT	1 cut(s)	1139
AclWI	GGATC	7 cut(s)	177, 310, 1429, 1444, 1457, 1473, 1566
AcoI	YGGCCR	2 cut(s)	10, 1676
AcsI	RAATTY	1 cut(s)	1591
AcuI	CTGAAG	1 cut(s)	1004
AcvI	CACGTG	1 cut(s)	579
AcyI	GRCGYC	2 cut(s)	243, 917
AfaI	GTAC	3 cut(s)	1297, 1523, 1538
AfiI	CCNNNNNNNGG	7 cut(s)	52, 388, 1192, 1480, 1481, 1578, 1766
AflIII	ACRYGT	5 cut(s)	312, 576, 1242, 1539, 1666
AgeI	ACCGGT	1 cut(s)	1215
AgsI	TTSAA	2 cut(s)	346, 1337
AhlI	ACTAGT	1 cut(s)	689
AjiI	CACGTC	2 cut(s)	538, 601
AjnI	CCWGG	6 cut(s)	435, 453, 502, 545, 669, 1678
AjuI	GAANNNNNNNTTGG	2 cut(s)	245, 277
AleI	CACNNNNGTG	1 cut(s)	1769
Alw21I	GWGCWC	3 cut(s)	453, 557, 1257
Alw26I	GTCTC	4 cut(s)	20, 51, 160, 868
Alw44I	GTGCAC	1 cut(s)	1253
AlwI	GGATC	7 cut(s)	177, 310, 1429, 1444, 1457, 1473, 1566
Ama87I	CYCGRG	8 cut(s)	298, 388, 446, 521, 706, 790, 1572, 1583
ApaLI	GTGCAC	1 cut(s)	1253
ApeKI	GCWGC	4 cut(s)	146, 355, 358, 1322
ApoI	RAATTY	1 cut(s)	1591
ArsI	GACNNNNNNTTYG	6 cut(s)	1124, 1156, 1257, 1289, 1295, 1327
AsiGI	ACCGGT	1 cut(s)	1215
AspLEI	GCGC	6 cut(s)	475, 483, 485, 502, 562, 919
AspS9I	GGNCC	8 cut(s)	412, 589, 608, 628, 643, 882, 1157, 1643
AsuC2I	CCSGG	7 cut(s)	33, 299, 300, 522, 523, 611, 1647
AsuHPI	GGTGA	4 cut(s)	625, 670, 1637, 1714
AvaI	CYCGRG	8 cut(s)	298, 388, 446, 521, 706, 790, 1572, 1583
AvaII	GGWCC	3 cut(s)	412, 643, 882
BaeGI	GKGCMC	1 cut(s)	1257
BaeI	ACNNNNGTAYC	1 cut(s)	31
BalI	TGGCCA	1 cut(s)	1678
BamHI	GGATCC	1 cut(s)	1449
BanI	GGYRCC	1 cut(s)	916
BanII	GRGCYC	2 cut(s)	453, 557
BbrPI	CACGTG	1 cut(s)	579
Bbv12I	GWGCWC	3 cut(s)	453, 557, 1257
BbvCI	CCTCAGC	1 cut(s)	365
BbvI	GCAGC	4 cut(s)	158, 342, 370, 1334
BccI	CCATC	7 cut(s)	137, 242, 403, 733, 789, 1394, 1572
BceAI	ACGGC	6 cut(s)	25, 137, 1042, 1190, 1310, 1407
BcgI	CGANNNNNNTGC	2 cut(s)	1210, 1244
BciT130I	CCWGG	6 cut(s)	437, 455, 504, 547, 671, 1680
BciVI	GTATCC	1 cut(s)	1226
BcnI	CCSGG	7 cut(s)	33, 299, 300, 522, 523, 611, 1647
BcoDI	GTCTC	4 cut(s)	20, 51, 160, 868
BcuI	ACTAGT	1 cut(s)	689
BfaI	CTAG	4 cut(s)	528, 690, 1527, 1607
BfmI	CTRYAG	2 cut(s)	356, 1656
BfoI	RGCGCY	2 cut(s)	563, 920
BfuI	GTATCC	1 cut(s)	1226
BlpI	GCTNAGC	1 cut(s)	551
Bme18I	GGWCC	3 cut(s)	412, 643, 882
BmeT110I	CYCGRG	8 cut(s)	298, 388, 446, 521, 706, 790, 1572, 1583
BmgBI	CACGTC	2 cut(s)	538, 601
BmgT120I	GGNCC	8 cut(s)	412, 589, 608, 628, 643, 882, 1157, 1643
BmiI	GGNNCC	5 cut(s)	40, 414, 883, 918, 1451
BmsI	GCATC	1 cut(s)	1103
BoxI	GACNNNNGTC	1 cut(s)	494
BplI	GAGNNNNNCTC	2 cut(s)	1566, 1598
BpmI	CTGGAG	1 cut(s)	389
Bpu10I	CCTNAGC	1 cut(s)	365
Bpu1102I	GCTNAGC	1 cut(s)	551
BpuEI	CTTGAG	1 cut(s)	623
BpuMI	CCSGG	7 cut(s)	33, 299, 300, 522, 523, 611, 1647
BsaAI	YACGTR	1 cut(s)	579
BsaBI	GATNNNNATC	2 cut(s)	1016, 1433
BsaHI	GRCGYC	2 cut(s)	243, 917
BsaI	GGTCTC	1 cut(s)	51
BsaWI	WCCGGW	3 cut(s)	1109, 1215, 1766
BsaXI	ACNNNNNCTCC	4 cut(s)	233, 263, 803, 833
Bsc4I	CCNNNNNNNGG	7 cut(s)	52, 388, 1192, 1480, 1481, 1578, 1766
Bse118I	RCCGGY	4 cut(s)	190, 832, 1154, 1215
Bse3DI	GCAATG	2 cut(s)	1122, 1324
Bse8I	GATNNNNATC	2 cut(s)	1016, 1433
BseBI	CCWGG	6 cut(s)	437, 455, 504, 547, 671, 1680
BseGI	GGATG	3 cut(s)	822, 1434, 1583
BseJI	GATNNNNATC	2 cut(s)	1016, 1433
BseLI	CCNNNNNNNGG	7 cut(s)	52, 388, 1192, 1480, 1481, 1578, 1766
BseMI	GCAATG	2 cut(s)	1122, 1324
BseMII	CTCAG	3 cut(s)	379, 542, 1388
BsePI	GCGCGC	1 cut(s)	481
BseRI	GAGGAG	7 cut(s)	31, 158, 200, 278, 376, 635, 774
BseSI	GKGCMC	1 cut(s)	1257
BseX3I	CGGCCG	1 cut(s)	10
BseXI	GCAGC	4 cut(s)	158, 342, 370, 1334
BseYI	CCCAGC	1 cut(s)	625
Bsh1236I	CGCG	9 cut(s)	314, 473, 481, 483, 500, 781, 1103, 1188, 1460
Bsh1285I	CGRYCG	2 cut(s)	13, 897
BshNI	GGYRCC	1 cut(s)	916
BshTI	ACCGGT	1 cut(s)	1215
BsiEI	CGRYCG	2 cut(s)	13, 897
BsiHKAI	GWGCWC	3 cut(s)	453, 557, 1257
BsiHKCI	CYCGRG	8 cut(s)	298, 388, 446, 521, 706, 790, 1572, 1583
BslFI	GGGAC	3 cut(s)	190, 895, 1145
BslI	CCNNNNNNNGG	7 cut(s)	52, 388, 1192, 1480, 1481, 1578, 1766
BsmAI	GTCTC	4 cut(s)	20, 51, 160, 868
BsmBI	CGTCTC	3 cut(s)	20, 160, 868
BsmFI	GGGAC	3 cut(s)	190, 895, 1145
BsmI	GAATGC	1 cut(s)	1775
Bso31I	GGTCTC	1 cut(s)	51
BsoBI	CYCGRG	8 cut(s)	298, 388, 446, 521, 706, 790, 1572, 1583
Bsp1286I	GDGCHC	3 cut(s)	453, 557, 1257
Bsp1407I	TGTACA	1 cut(s)	1536
Bsp143I	GATC	9 cut(s)	182, 302, 894, 906, 1198, 1434, 1449, 1465, 1558
Bsp1720I	GCTNAGC	1 cut(s)	551
Bsp19I	CCATGG	1 cut(s)	51
BspCNI	CTCAG	3 cut(s)	378, 543, 1387
BspFNI	CGCG	9 cut(s)	314, 473, 481, 483, 500, 781, 1103, 1188, 1460
BspLI	GGNNCC	5 cut(s)	40, 414, 883, 918, 1451
BspMAI	CTGCAG	1 cut(s)	360
BspPI	GGATC	7 cut(s)	177, 310, 1429, 1444, 1457, 1473, 1566
BspT107I	GGYRCC	1 cut(s)	916
BspTNI	GGTCTC	1 cut(s)	51
BsrBI	CCGCTC	2 cut(s)	48, 606
BsrDI	GCAATG	2 cut(s)	1122, 1324
BsrFI	RCCGGY	4 cut(s)	190, 832, 1154, 1215
BsrGI	TGTACA	1 cut(s)	1536
BssAI	RCCGGY	4 cut(s)	190, 832, 1154, 1215
BssHII	GCGCGC	1 cut(s)	481
BssMI	GATC	9 cut(s)	182, 302, 894, 906, 1198, 1434, 1449, 1465, 1558
BssNI	GRCGYC	2 cut(s)	243, 917
BssT1I	CCWWGG	3 cut(s)	51, 867, 1511
Bst2UI	CCWGG	6 cut(s)	437, 455, 504, 547, 671, 1680
BstACI	GRCGYC	2 cut(s)	243, 917
BstAPI	GCANNNNNTGC	1 cut(s)	1202
BstAUI	TGTACA	1 cut(s)	1536
BstBAI	YACGTR	1 cut(s)	579
BstDEI	CTNAG	3 cut(s)	365, 551, 1374
BstDSI	CCRYGG	3 cut(s)	51, 1206, 1326
BstEII	GGTNACC	1 cut(s)	1755
BstF5I	GGATG	3 cut(s)	822, 1434, 1583
BstFNI	CGCG	9 cut(s)	314, 473, 481, 483, 500, 781, 1103, 1188, 1460
BstH2I	RGCGCY	2 cut(s)	563, 920
BstHHI	GCGC	6 cut(s)	475, 483, 485, 502, 562, 919
BstKTI	GATC	9 cut(s)	185, 305, 897, 909, 1201, 1437, 1452, 1468, 1561
BstMAI	GTCTC	4 cut(s)	20, 51, 160, 868
BstMBI	GATC	9 cut(s)	182, 302, 894, 906, 1198, 1434, 1449, 1465, 1558
BstMCI	CGRYCG	2 cut(s)	13, 897
BstNI	CCWGG	6 cut(s)	437, 455, 504, 547, 671, 1680
BstNSI	RCATGY	1 cut(s)	1543
BstPAI	GACNNNNGTC	1 cut(s)	494
BstPI	GGTNACC	1 cut(s)	1755
BstSFI	CTRYAG	2 cut(s)	356, 1656
BstSLI	GKGCMC	1 cut(s)	1257
BstUI	CGCG	9 cut(s)	314, 473, 481, 483, 500, 781, 1103, 1188, 1460
BstV1I	GCAGC	4 cut(s)	158, 342, 370, 1334
BstX2I	RGATCY	3 cut(s)	302, 1449, 1465
BstXI	CCANNNNNNTGG	1 cut(s)	1771
BstYI	RGATCY	3 cut(s)	302, 1449, 1465
BstZI	CGGCCG	1 cut(s)	10
BsuI	GTATCC	1 cut(s)	1226
BtgI	CCRYGG	3 cut(s)	51, 1206, 1326
BtgZI	GCGATG	2 cut(s)	124, 157
BtrI	CACGTC	2 cut(s)	538, 601
BtsCI	GGATG	3 cut(s)	822, 1434, 1583
CfoI	GCGC	6 cut(s)	475, 483, 485, 502, 562, 919
Cfr10I	RCCGGY	4 cut(s)	190, 832, 1154, 1215
Cfr13I	GGNCC	8 cut(s)	412, 589, 608, 628, 643, 882, 1157, 1643
Cfr9I	CCCGGG	2 cut(s)	298, 521
CseI	GACGC	7 cut(s)	235, 251, 265, 303, 361, 479, 1449
Csp6I	GTAC	3 cut(s)	1296, 1522, 1537
CspAI	ACCGGT	1 cut(s)	1215
CviAII	CATG	7 cut(s)	52, 349, 1202, 1540, 1562, 1633, 1752
CviQI	GTAC	3 cut(s)	1296, 1522, 1537
DdeI	CTNAG	3 cut(s)	365, 551, 1374
DinI	GGCGCC	1 cut(s)	918
DpnI	GATC	9 cut(s)	184, 304, 896, 908, 1200, 1436, 1451, 1467, 1560
DpnII	GATC	9 cut(s)	182, 302, 894, 906, 1198, 1434, 1449, 1465, 1558
EaeI	YGGCCR	2 cut(s)	10, 1676
EagI	CGGCCG	1 cut(s)	10
EciI	GGCGGA	1 cut(s)	418
Ecl136II	GAGCTC	2 cut(s)	451, 555
EclXI	CGGCCG	1 cut(s)	10
Eco130I	CCWWGG	3 cut(s)	51, 867, 1511
Eco24I	GRGCYC	2 cut(s)	453, 557
Eco31I	GGTCTC	1 cut(s)	51
Eco47I	GGWCC	3 cut(s)	412, 643, 882
Eco52I	CGGCCG	1 cut(s)	10
Eco53kI	GAGCTC	2 cut(s)	451, 555
Eco57I	CTGAAG	1 cut(s)	1004
Eco72I	CACGTG	1 cut(s)	579
Eco88I	CYCGRG	8 cut(s)	298, 388, 446, 521, 706, 790, 1572, 1583
Eco91I	GGTNACC	1 cut(s)	1755
EcoICRI	GAGCTC	2 cut(s)	451, 555
EcoO109I	RGGNCCY	3 cut(s)	589, 643, 882
EcoO65I	GGTNACC	1 cut(s)	1755
EcoRI	GAATTC	1 cut(s)	1591
EcoRII	CCWGG	6 cut(s)	435, 453, 502, 545, 669, 1678
EcoT14I	CCWWGG	3 cut(s)	51, 867, 1511
EcoT38I	GRGCYC	2 cut(s)	453, 557
EgeI	GGCGCC	1 cut(s)	918
EheI	GGCGCC	1 cut(s)	918
ErhI	CCWWGG	3 cut(s)	51, 867, 1511
Esp3I	CGTCTC	3 cut(s)	20, 160, 868
FaeI	CATG	7 cut(s)	55, 352, 1205, 1543, 1565, 1636, 1755
FalI	AAGNNNNNCTT	4 cut(s)	245, 277, 1472, 1504
FaqI	GGGAC	3 cut(s)	190, 895, 1145
FatI	CATG	7 cut(s)	51, 348, 1201, 1539, 1561, 1632, 1751
FauI	CCCGC	7 cut(s)	53, 75, 575, 774, 1167, 1364, 1643
FblI	GTMKAC	1 cut(s)	1212
FokI	GGATG	3 cut(s)	829, 1441, 1590
FriOI	GRGCYC	2 cut(s)	453, 557
FspBI	CTAG	4 cut(s)	528, 690, 1527, 1607
GlaI	GCGC	6 cut(s)	474, 482, 484, 501, 561, 918
GsaI	CCCAGC	1 cut(s)	629
GsuI	CTGGAG	1 cut(s)	389
HaeII	RGCGCY	2 cut(s)	563, 920
HgaI	GACGC	7 cut(s)	235, 251, 265, 303, 361, 479, 1449
HhaI	GCGC	6 cut(s)	475, 483, 485, 502, 562, 919
Hin1I	GRCGYC	2 cut(s)	243, 917
Hin1II	CATG	7 cut(s)	55, 352, 1205, 1543, 1565, 1636, 1755
Hin6I	GCGC	6 cut(s)	473, 481, 483, 500, 560, 917
HinP1I	GCGC	6 cut(s)	473, 481, 483, 500, 560, 917
HincII	GTYRAC	2 cut(s)	280, 1664
HindII	GTYRAC	2 cut(s)	280, 1664
HinfI	GANTC	5 cut(s)	256, 461, 507, 718, 1414
HphI	GGTGA	4 cut(s)	625, 670, 1637, 1714
Hpy188III	TCNNGA	6 cut(s)	406, 572, 708, 790, 1277, 1607
Hpy99I	CGWCG	8 cut(s)	229, 319, 500, 515, 602, 1028, 1465, 1498
HpyAV	CCTTC	6 cut(s)	276, 352, 802, 957, 979, 987
HpyCH4IV	ACGT	9 cut(s)	537, 578, 600, 951, 1139, 1244, 1496, 1666, 1697
HpyCH4V	TGCA	6 cut(s)	358, 1127, 1221, 1255, 1322, 1730
HpyF3I	CTNAG	3 cut(s)	365, 551, 1374
HpySE526I	ACGT	9 cut(s)	537, 578, 600, 951, 1139, 1244, 1496, 1666, 1697
Hsp92I	GRCGYC	2 cut(s)	243, 917
Hsp92II	CATG	7 cut(s)	55, 352, 1205, 1543, 1565, 1636, 1755
HspAI	GCGC	6 cut(s)	473, 481, 483, 500, 560, 917
KasI	GGCGCC	1 cut(s)	916
KroI	GCCGGC	2 cut(s)	190, 1154
KroNI	GCCGGC	2 cut(s)	192, 1156
Kzo9I	GATC	9 cut(s)	182, 302, 894, 906, 1198, 1434, 1449, 1465, 1558
LmnI	GCTCC	8 cut(s)	44, 53, 254, 456, 622, 652, 1112, 1573
Lsp1109I	GCAGC	4 cut(s)	158, 342, 370, 1334
LweI	GCATC	1 cut(s)	1103
MaeI	CTAG	4 cut(s)	528, 690, 1527, 1607
MaeII	ACGT	9 cut(s)	537, 578, 600, 951, 1139, 1244, 1496, 1666, 1697
MaeIII	GTNAC	4 cut(s)	662, 695, 1283, 1755
MalI	GATC	9 cut(s)	184, 304, 896, 908, 1200, 1436, 1451, 1467, 1560
MbiI	CCGCTC	2 cut(s)	48, 606
MboI	GATC	9 cut(s)	182, 302, 894, 906, 1198, 1434, 1449, 1465, 1558
MboII	GAAGA	3 cut(s)	983, 986, 1009
MflI	RGATCY	3 cut(s)	302, 1449, 1465
MhlI	GDGCHC	3 cut(s)	453, 557, 1257
MlsI	TGGCCA	1 cut(s)	1678
MluCI	AATT	4 cut(s)	1339, 1591, 1597, 1692
MluI	ACGCGT	1 cut(s)	312
MluNI	TGGCCA	1 cut(s)	1678
Mly113I	GGCGCC	1 cut(s)	917
MlyI	GAGTC	3 cut(s)	250, 455, 501
MmeI	TCCRAC	6 cut(s)	43, 51, 297, 630, 1443, 1517
Mox20I	TGGCCA	1 cut(s)	1678
MroNI	GCCGGC	2 cut(s)	190, 1154
MscI	TGGCCA	1 cut(s)	1678
MslI	CAYNNNNRTG	2 cut(s)	1427, 1769
Msp20I	TGGCCA	1 cut(s)	1678
MspA1I	CMGCKG	1 cut(s)	1050
Mva1269I	GAATGC	1 cut(s)	1775
MvaI	CCWGG	6 cut(s)	437, 455, 504, 547, 671, 1680
MvnI	CGCG	9 cut(s)	314, 473, 481, 483, 500, 781, 1103, 1188, 1460
NaeI	GCCGGC	2 cut(s)	192, 1156
NarI	GGCGCC	1 cut(s)	917
NciI	CCSGG	7 cut(s)	33, 299, 300, 522, 523, 611, 1647
NcoI	CCATGG	1 cut(s)	51
NdeII	GATC	9 cut(s)	182, 302, 894, 906, 1198, 1434, 1449, 1465, 1558
NgoMIV	GCCGGC	2 cut(s)	190, 1154
NlaIII	CATG	7 cut(s)	55, 352, 1205, 1543, 1565, 1636, 1755
NlaIV	GGNNCC	5 cut(s)	40, 414, 883, 918, 1451
NmeAIII	GCCGAG	2 cut(s)	536, 925
NmuCI	GTSAC	1 cut(s)	1283
NspI	RCATGY	1 cut(s)	1543
OliI	CACNNNNGTG	1 cut(s)	1769
PaeR7I	CTCGAG	2 cut(s)	446, 1583
PauI	GCGCGC	1 cut(s)	481
PciI	ACATGT	1 cut(s)	1539
PcsI	WCGNNNNNNNCGW	4 cut(s)	395, 713, 1185, 1430
PctI	GAATGC	1 cut(s)	1775
PdiI	GCCGGC	2 cut(s)	192, 1156
PfeI	GAWTC	2 cut(s)	718, 1414
PflFI	GACNNNGTC	1 cut(s)	329
PfoI	TCCNGGA	1 cut(s)	453
PinAI	ACCGGT	1 cut(s)	1215
Ple19I	CGATCG	1 cut(s)	897
PleI	GAGTC	3 cut(s)	250, 455, 501
PluTI	GGCGCC	1 cut(s)	920
PmaCI	CACGTG	1 cut(s)	579
PmlI	CACGTG	1 cut(s)	579
PpsI	GAGTC	3 cut(s)	250, 455, 501
Ppu21I	YACGTR	1 cut(s)	579
PpuMI	RGGWCCY	2 cut(s)	643, 882
PscI	ACATGT	1 cut(s)	1539
PshAI	GACNNNNGTC	1 cut(s)	494
Psp124BI	GAGCTC	2 cut(s)	453, 557
Psp1406I	AACGTT	1 cut(s)	1139
Psp5II	RGGWCCY	2 cut(s)	643, 882
Psp6I	CCWGG	6 cut(s)	435, 453, 502, 545, 669, 1678
PspCI	CACGTG	1 cut(s)	579
PspEI	GGTNACC	1 cut(s)	1755
PspFI	CCCAGC	1 cut(s)	625
PspGI	CCWGG	6 cut(s)	435, 453, 502, 545, 669, 1678
PspN4I	GGNNCC	5 cut(s)	40, 414, 883, 918, 1451
PspPI	GGNCC	8 cut(s)	412, 589, 608, 628, 643, 882, 1157, 1643
PspPPI	RGGWCCY	2 cut(s)	643, 882
PspXI	VCTCGAGB	2 cut(s)	446, 1583
PstI	CTGCAG	1 cut(s)	360
PsuI	RGATCY	3 cut(s)	302, 1449, 1465
PsyI	GACNNNGTC	1 cut(s)	329
PteI	GCGCGC	1 cut(s)	481
PvuI	CGATCG	1 cut(s)	897
RsaI	GTAC	3 cut(s)	1297, 1523, 1538
RsaNI	GTAC	3 cut(s)	1296, 1522, 1537
RseI	CAYNNNNRTG	2 cut(s)	1427, 1769
SacI	GAGCTC	2 cut(s)	453, 557
Sau3AI	GATC	9 cut(s)	182, 302, 894, 906, 1198, 1434, 1449, 1465, 1558
Sau96I	GGNCC	8 cut(s)	412, 589, 608, 628, 643, 882, 1157, 1643
SchI	GAGTC	3 cut(s)	250, 455, 501
SduI	GDGCHC	3 cut(s)	453, 557, 1257
SfaNI	GCATC	1 cut(s)	1103
SfcI	CTRYAG	2 cut(s)	356, 1656
SfoI	GGCGCC	1 cut(s)	918
Sfr274I	CTCGAG	2 cut(s)	446, 1583
SinI	GGWCC	3 cut(s)	412, 643, 882
SlaI	CTCGAG	2 cut(s)	446, 1583
SmaI	CCCGGG	2 cut(s)	300, 523
SmiMI	CAYNNNNRTG	2 cut(s)	1427, 1769
SmlI	CTYRAG	3 cut(s)	446, 638, 1583
SmoI	CTYRAG	3 cut(s)	446, 638, 1583
SpeI	ACTAGT	1 cut(s)	689
Sse9I	AATT	4 cut(s)	1339, 1591, 1597, 1692
SspDI	GGCGCC	1 cut(s)	916
SspMI	CTAG	4 cut(s)	528, 690, 1527, 1607
SstI	GAGCTC	2 cut(s)	453, 557
StyI	CCWWGG	3 cut(s)	51, 867, 1511
TaiI	ACGT	9 cut(s)	540, 581, 603, 954, 1142, 1247, 1499, 1669, 1700
TaqI	TCGA	7 cut(s)	197, 291, 447, 510, 1259, 1433, 1584
TaqII	GACCGA	1 cut(s)	1783
TasI	AATT	4 cut(s)	1339, 1591, 1597, 1692
TatI	WGTACW	2 cut(s)	1521, 1536
TauI	GCSGC	6 cut(s)	73, 146, 152, 609, 1106, 1460
TfiI	GAWTC	2 cut(s)	718, 1414
TseFI	GTSAC	1 cut(s)	1283
TseI	GCWGC	4 cut(s)	146, 355, 358, 1322
Tsp45I	GTSAC	1 cut(s)	1283
TspDTI	ATGAA	5 cut(s)	984, 1006, 1111, 1297, 1649
TspGWI	ACGGA	1 cut(s)	888
TspMI	CCCGGG	2 cut(s)	298, 521
Tth111I	GACNNNGTC	1 cut(s)	329
VneI	GTGCAC	1 cut(s)	1253
VpaK11BI	GGWCC	3 cut(s)	412, 643, 882
XapI	RAATTY	1 cut(s)	1591
XbaI	TCTAGA	1 cut(s)	1606
XceI	RCATGY	1 cut(s)	1543
XhoI	CTCGAG	2 cut(s)	446, 1583
XmaI	CCCGGG	2 cut(s)	298, 521
XmiI	GTMKAC	1 cut(s)	1212
XspI	CTAG	4 cut(s)	528, 690, 1527, 1607

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.