Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0007564

Physical Location & Seq

Genomic Coordinates Reverse (-)

66273394 .. 66277265

Gene Structure 3872 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ60398

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1806 bp

ATGGGATACGGCCGTCTCCAACCCTCCGACCCCGGAGGCTCCTCCCGCTCCATGGGCGACCAATCCCCGCCGCAACAATCACCGTCTCGTAAAAGCAAGAAAAAGCTCATCATACTCTCCATTCTCTCCATCACTCTCATCGCCGCAGCCGCTGTCTCCGCAGTCCTCCTCATCGCCGTCCGATCCAAGCCGGCCGCGAAGCAGTCCCTCCTCCACCGCCAGCCGACGCAAGCCATCTCTGACGCCTGCTCCAAGACTCGCTTCCCTTCTCTCTGCGTCAACTCCCTCCTCGACTTCCCGGGATCTCTGAACGCTTCGGAGACCGACCTCGTCCACATTTCCTTCAACATGACGCTGCAGCACCTCAGCAAAGCCCTCTACCTCTCCTCGGGGCTATCGTATCTCCAGATGGACCCACACGCCAAGTCCGCCTACCAGGACTGCCTCGAGCTCCTGGACGACTCTGTGGACGCGCTCACGCGCGCCCTGACTACCGTCGCGCCTGTGCCTGGACGCGACGGTAGCCTCGGGTCTAGCCAGGACGTGCTGACCTGGCTGAGCTCGGCGCTGACAAATCAGGACACGTGCGGGGAGGGCCTCGCCGACGTGAGCGGCCCGGTGAAAGAGGAGCTGGGCCAGAAGCTCAAGGACCTGTCGGAGCTGGTGAGTAACTGCCTGGCGATATTTACAGCGACTAGTAGTAACGACTTCTCGGGAGTGCCGATTCAGAACAGGAGATTGCTGATGGAGAGTGATATAATGGGAGGAGATAATGCCGATGTGTCGCGGGATTTCCCGAGATGGTTGGGTAAGAAGGAGAGGATGTTGCTGGCTTTGCCGGTGGCACAGGTACAGGCCGATATAATCGTCTCCAAGGACGGAAATGGGACCTACAAGACGATCGCCGAGGCGATCAAAAAGGCGCCGGAGTATAGTAAACGCCGGACAGTTATTTACGTCAGGGCAGGAAGGTATGAAGAAGATAATCTGAAGGTAGGAAGGAAGAAATGGAACTTGATGTTCATCGGCGACGGCAAGGGTATAACCATAATCAGCGGTGGTAAAAGCGTTTCCGAAAAGCTAACCACATTCCGCACCGCATCCTTCGCGGCTACCGGAGCAGGCTTCATTGCACGGGACATAACGTTTGAGAACTATGCCGGGCCGCAGAAGCACCAAGCCGTCGCCCTCCGCGTTGGGGCAGATCATGCCGTGGTCTACCGGTGCAACATAATCGGATACCAAGACACGTTTTATGTGCACTCCAACAGACAGTTCGTTCGGGAATGTGACATTTATGGTACTGTGGACTTCATATTTGGCAATGCAGCCGTGGTGTTTCAAAATTGTAGTATCTACGCCCGCAAGCCAATGCCCTCTCAGAAAAACACCATCACGGCTCAAAACAGGAAAGACCCGAATCAAAACACGGGGATGTCGATCCACGCTTGCCGGATCCTTGCAGCGTCGGATCTTGCCCCCGTAAAGGGTAGCTTTCCGACGTATTTGGGTCGCCCTTGGAAAATGTACTCTAGGGTTGTGTACATGTTATCATACATTGGGGATCATGTAGCTCCCCGAGGATGGCTCGAGTGGAATTCCAATTTTGCCCTAGATACTCTCTACTACGGTGAATACATGAACTATGGGCCGGGAGGGGCTGTAGGTCAACGTGTAAAATGGCCAGGGTATCGGGTCATTACGTCCACCGTTGAGGCCGACAAGTTCACCGTTGCACGGTTTATATATGGCTCATCATGGTTACCCTCCACCGGAGTGGCATTCTTGGGTGGTCTTTCGGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0010119 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050789 GO:0050790 GO:0050794 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

601

Amino Acids

65.61

Weight (kDa)

9.18

Isoelectric Point (pI)

41.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	78 - 228	3.4e-35	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	287 - 584	4.3e-144	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49220 AT3G49220 AT3G49220 AT5G53370 AT5G53370
fragaria_vesca	FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650
malus_domestica	MD01G1220700.v1.1 MD07G1289200.v1.1
prunus_persica	Prupe.2G310600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0380731
rosa_laevigata	RLG00000026273
rosa_multiflora	Rmu_sc0001637.1_g000012
rosa_roxburghii	Rroxscaffold_4G00279070
rosa_rugosa	Rorug01G0424000
rosa_samantha	Rh1AG448200 Rh1BG403600 Rh1CG417900 Rh1DG433900
rosa_wichuraiana	Rw0G005890 Rw1G039010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	922
AccBSI	CCGCTC	2 cut(s)	48, 612
AccI	GTMKAC	1 cut(s)	1218
AccII	CGCG	9 cut(s)	197, 473, 481, 483, 500, 516, 787, 1109, 1194
AclI	AACGTT	1 cut(s)	1145
AclWI	GGATC	7 cut(s)	177, 310, 1435, 1450, 1463, 1479, 1572
AcoI	YGGCCR	3 cut(s)	10, 192, 1682
AcsI	RAATTY	1 cut(s)	1597
AcuI	CTGAAG	1 cut(s)	1010
AcvI	CACGTG	1 cut(s)	585
AcyI	GRCGYC	2 cut(s)	243, 923
AfaI	GTAC	4 cut(s)	852, 1303, 1529, 1544
AfiI	CCNNNNNNNGG	9 cut(s)	52, 388, 509, 1198, 1486, 1487, 1584, 1737, 1772
AflIII	ACRYGT	4 cut(s)	582, 1248, 1545, 1672
AgeI	ACCGGT	1 cut(s)	1221
AgsI	TTSAA	2 cut(s)	346, 1343
AhlI	ACTAGT	1 cut(s)	695
AjiI	CACGTC	2 cut(s)	544, 607
AjnI	CCWGG	7 cut(s)	435, 453, 508, 537, 551, 675, 1684
AjuI	GAANNNNNNNTTGG	4 cut(s)	245, 277, 1259, 1291
AleI	CACNNNNGTG	1 cut(s)	1775
AluBI	AGCT	9 cut(s)	106, 451, 561, 631, 643, 661, 1081, 1494, 1574
AluI	AGCT	9 cut(s)	106, 451, 561, 631, 643, 661, 1081, 1494, 1574
Alw21I	GWGCWC	3 cut(s)	453, 563, 1263
Alw26I	GTCTC	5 cut(s)	20, 90, 160, 314, 874
Alw44I	GTGCAC	1 cut(s)	1259
AlwI	GGATC	7 cut(s)	177, 310, 1435, 1450, 1463, 1479, 1572
AlwNI	CAGNNNCTG	1 cut(s)	152
Ama87I	CYCGRG	8 cut(s)	298, 388, 446, 527, 712, 796, 1578, 1589
ApaLI	GTGCAC	1 cut(s)	1259
ApeKI	GCWGC	5 cut(s)	146, 355, 358, 1328, 1463
ApoI	RAATTY	1 cut(s)	1597
ArsI	GACNNNNNNTTYG	6 cut(s)	1130, 1162, 1263, 1295, 1301, 1333
AsiGI	ACCGGT	1 cut(s)	1221
AspLEI	GCGC	6 cut(s)	475, 483, 485, 502, 568, 925
AspS9I	GGNCC	8 cut(s)	412, 595, 614, 634, 649, 888, 1163, 1649
AsuC2I	CCSGG	6 cut(s)	33, 299, 300, 617, 1162, 1653
AsuHPI	GGTGA	5 cut(s)	72, 631, 676, 1643, 1720
AvaI	CYCGRG	8 cut(s)	298, 388, 446, 527, 712, 796, 1578, 1589
AvaII	GGWCC	3 cut(s)	412, 649, 888
BaeGI	GKGCMC	1 cut(s)	1263
BaeI	ACNNNNGTAYC	1 cut(s)	31
BalI	TGGCCA	1 cut(s)	1684
BamHI	GGATCC	1 cut(s)	1455
BanI	GGYRCC	1 cut(s)	922
BanII	GRGCYC	2 cut(s)	453, 563
BbrPI	CACGTG	1 cut(s)	585
Bbv12I	GWGCWC	3 cut(s)	453, 563, 1263
BbvCI	CCTCAGC	1 cut(s)	365
BbvI	GCAGC	5 cut(s)	158, 342, 370, 1340, 1475
BccI	CCATC	7 cut(s)	137, 242, 403, 739, 795, 1400, 1578
BceAI	ACGGC	7 cut(s)	25, 161, 1048, 1166, 1196, 1316, 1413
BcgI	CGANNNNNNTGC	2 cut(s)	1216, 1250
BciT130I	CCWGG	7 cut(s)	437, 455, 510, 539, 553, 677, 1686
BciVI	GTATCC	1 cut(s)	1232
BcnI	CCSGG	6 cut(s)	33, 299, 300, 617, 1162, 1653
BcoDI	GTCTC	5 cut(s)	20, 90, 160, 314, 874
BcuI	ACTAGT	1 cut(s)	695
BfaI	CTAG	4 cut(s)	534, 696, 1533, 1613
BfmI	CTRYAG	2 cut(s)	356, 1662
BfoI	RGCGCY	2 cut(s)	569, 926
BfuI	GTATCC	1 cut(s)	1232
BlpI	GCTNAGC	1 cut(s)	557
Bme18I	GGWCC	3 cut(s)	412, 649, 888
BmeT110I	CYCGRG	8 cut(s)	298, 388, 446, 527, 712, 796, 1578, 1589
BmgBI	CACGTC	2 cut(s)	544, 607
BmgT120I	GGNCC	8 cut(s)	412, 595, 614, 634, 649, 888, 1163, 1649
BmiI	GGNNCC	5 cut(s)	40, 414, 889, 924, 1457
BmsI	GCATC	1 cut(s)	1109
BoxI	GACNNNNGTC	1 cut(s)	494
BplI	GAGNNNNNCTC	2 cut(s)	1572, 1604
BpmI	CTGGAG	1 cut(s)	389
Bpu10I	CCTNAGC	1 cut(s)	365
Bpu1102I	GCTNAGC	1 cut(s)	557
BpuEI	CTTGAG	1 cut(s)	629
BpuMI	CCSGG	6 cut(s)	33, 299, 300, 617, 1162, 1653
BsaAI	YACGTR	1 cut(s)	585
BsaBI	GATNNNNATC	2 cut(s)	1022, 1439
BsaHI	GRCGYC	2 cut(s)	243, 923
BsaI	GGTCTC	1 cut(s)	314
BsaWI	WCCGGW	3 cut(s)	1115, 1221, 1772
BsaXI	ACNNNNNCTCC	6 cut(s)	233, 263, 809, 839, 1647, 1677
Bsc4I	CCNNNNNNNGG	9 cut(s)	52, 388, 509, 1198, 1486, 1487, 1584, 1737, 1772
Bse118I	RCCGGY	3 cut(s)	190, 838, 1221
Bse3DI	GCAATG	2 cut(s)	1128, 1330
Bse8I	GATNNNNATC	2 cut(s)	1022, 1439
BseBI	CCWGG	7 cut(s)	437, 455, 510, 539, 553, 677, 1686
BseGI	GGATG	4 cut(s)	828, 1100, 1440, 1589
BseJI	GATNNNNATC	2 cut(s)	1022, 1439
BseLI	CCNNNNNNNGG	9 cut(s)	52, 388, 509, 1198, 1486, 1487, 1584, 1737, 1772
BseMI	GCAATG	2 cut(s)	1128, 1330
BseMII	CTCAG	3 cut(s)	379, 548, 1394
BsePI	GCGCGC	1 cut(s)	481
BseRI	GAGGAG	7 cut(s)	31, 158, 200, 278, 376, 641, 780
BseSI	GKGCMC	1 cut(s)	1263
BseX3I	CGGCCG	2 cut(s)	10, 192
BseXI	GCAGC	5 cut(s)	158, 342, 370, 1340, 1475
BseYI	CCCAGC	1 cut(s)	631
Bsh1236I	CGCG	9 cut(s)	197, 473, 481, 483, 500, 516, 787, 1109, 1194
Bsh1285I	CGRYCG	3 cut(s)	13, 195, 903
BshNI	GGYRCC	1 cut(s)	922
BshTI	ACCGGT	1 cut(s)	1221
BsiEI	CGRYCG	3 cut(s)	13, 195, 903
BsiHKAI	GWGCWC	3 cut(s)	453, 563, 1263
BsiHKCI	CYCGRG	8 cut(s)	298, 388, 446, 527, 712, 796, 1578, 1589
BslFI	GGGAC	3 cut(s)	190, 901, 1151
BslI	CCNNNNNNNGG	9 cut(s)	52, 388, 509, 1198, 1486, 1487, 1584, 1737, 1772
BsmAI	GTCTC	5 cut(s)	20, 90, 160, 314, 874
BsmBI	CGTCTC	3 cut(s)	20, 90, 874
BsmFI	GGGAC	3 cut(s)	190, 901, 1151
BsmI	GAATGC	1 cut(s)	1781
Bso31I	GGTCTC	1 cut(s)	314
BsoBI	CYCGRG	8 cut(s)	298, 388, 446, 527, 712, 796, 1578, 1589
Bsp1286I	GDGCHC	3 cut(s)	453, 563, 1263
Bsp1407I	TGTACA	1 cut(s)	1542
Bsp143I	GATC	9 cut(s)	182, 302, 900, 912, 1204, 1440, 1455, 1471, 1564
Bsp1720I	GCTNAGC	1 cut(s)	557
Bsp19I	CCATGG	1 cut(s)	51
BspCNI	CTCAG	3 cut(s)	378, 549, 1393
BspFNI	CGCG	9 cut(s)	197, 473, 481, 483, 500, 516, 787, 1109, 1194
BspLI	GGNNCC	5 cut(s)	40, 414, 889, 924, 1457
BspMAI	CTGCAG	1 cut(s)	360
BspPI	GGATC	7 cut(s)	177, 310, 1435, 1450, 1463, 1479, 1572
BspT107I	GGYRCC	1 cut(s)	922
BspTNI	GGTCTC	1 cut(s)	314
BsrBI	CCGCTC	2 cut(s)	48, 612
BsrDI	GCAATG	2 cut(s)	1128, 1330
BsrFI	RCCGGY	3 cut(s)	190, 838, 1221
BsrGI	TGTACA	1 cut(s)	1542
BssAI	RCCGGY	3 cut(s)	190, 838, 1221
BssHII	GCGCGC	1 cut(s)	481
BssMI	GATC	9 cut(s)	182, 302, 900, 912, 1204, 1440, 1455, 1471, 1564
BssNI	GRCGYC	2 cut(s)	243, 923
BssT1I	CCWWGG	3 cut(s)	51, 873, 1517
Bst2UI	CCWGG	7 cut(s)	437, 455, 510, 539, 553, 677, 1686
BstACI	GRCGYC	2 cut(s)	243, 923
BstAPI	GCANNNNNTGC	1 cut(s)	1208
BstAUI	TGTACA	1 cut(s)	1542
BstBAI	YACGTR	1 cut(s)	585
BstDEI	CTNAG	3 cut(s)	365, 557, 1380
BstDSI	CCRYGG	3 cut(s)	51, 1212, 1332
BstEII	GGTNACC	1 cut(s)	1761
BstF5I	GGATG	4 cut(s)	828, 1100, 1440, 1589
BstFNI	CGCG	9 cut(s)	197, 473, 481, 483, 500, 516, 787, 1109, 1194
BstH2I	RGCGCY	2 cut(s)	569, 926
BstHHI	GCGC	6 cut(s)	475, 483, 485, 502, 568, 925
BstKTI	GATC	9 cut(s)	185, 305, 903, 915, 1207, 1443, 1458, 1474, 1567
BstMAI	GTCTC	5 cut(s)	20, 90, 160, 314, 874
BstMBI	GATC	9 cut(s)	182, 302, 900, 912, 1204, 1440, 1455, 1471, 1564
BstMCI	CGRYCG	3 cut(s)	13, 195, 903
BstNI	CCWGG	7 cut(s)	437, 455, 510, 539, 553, 677, 1686
BstNSI	RCATGY	1 cut(s)	1549
BstPAI	GACNNNNGTC	1 cut(s)	494
BstPI	GGTNACC	1 cut(s)	1761
BstSFI	CTRYAG	2 cut(s)	356, 1662
BstSLI	GKGCMC	1 cut(s)	1263
BstUI	CGCG	9 cut(s)	197, 473, 481, 483, 500, 516, 787, 1109, 1194
BstV1I	GCAGC	5 cut(s)	158, 342, 370, 1340, 1475
BstX2I	RGATCY	3 cut(s)	302, 1455, 1471
BstXI	CCANNNNNNTGG	1 cut(s)	1777
BstYI	RGATCY	3 cut(s)	302, 1455, 1471
BstZI	CGGCCG	2 cut(s)	10, 192
BsuI	GTATCC	1 cut(s)	1232
BtgI	CCRYGG	3 cut(s)	51, 1212, 1332
BtgZI	GCGATG	2 cut(s)	124, 157
BtrI	CACGTC	2 cut(s)	544, 607
BtsCI	GGATG	4 cut(s)	828, 1100, 1440, 1589
CaiI	CAGNNNCTG	1 cut(s)	152
CfoI	GCGC	6 cut(s)	475, 483, 485, 502, 568, 925
Cfr10I	RCCGGY	3 cut(s)	190, 838, 1221
Cfr13I	GGNCC	8 cut(s)	412, 595, 614, 634, 649, 888, 1163, 1649
Cfr9I	CCCGGG	1 cut(s)	298
CseI	GACGC	7 cut(s)	235, 251, 265, 361, 479, 522, 1455
Csp6I	GTAC	4 cut(s)	851, 1302, 1528, 1543
CspAI	ACCGGT	1 cut(s)	1221
CviAII	CATG	7 cut(s)	52, 349, 1208, 1546, 1568, 1639, 1758
CviQI	GTAC	4 cut(s)	851, 1302, 1528, 1543
DdeI	CTNAG	3 cut(s)	365, 557, 1380
DinI	GGCGCC	1 cut(s)	924
DpnI	GATC	9 cut(s)	184, 304, 902, 914, 1206, 1442, 1457, 1473, 1566
DpnII	GATC	9 cut(s)	182, 302, 900, 912, 1204, 1440, 1455, 1471, 1564
EaeI	YGGCCR	3 cut(s)	10, 192, 1682
EagI	CGGCCG	2 cut(s)	10, 192
EciI	GGCGGA	1 cut(s)	418
Ecl136II	GAGCTC	2 cut(s)	451, 561
EclXI	CGGCCG	2 cut(s)	10, 192
Eco130I	CCWWGG	3 cut(s)	51, 873, 1517
Eco24I	GRGCYC	2 cut(s)	453, 563
Eco31I	GGTCTC	1 cut(s)	314
Eco47I	GGWCC	3 cut(s)	412, 649, 888
Eco52I	CGGCCG	2 cut(s)	10, 192
Eco53kI	GAGCTC	2 cut(s)	451, 561
Eco57I	CTGAAG	1 cut(s)	1010
Eco72I	CACGTG	1 cut(s)	585
Eco88I	CYCGRG	8 cut(s)	298, 388, 446, 527, 712, 796, 1578, 1589
Eco91I	GGTNACC	1 cut(s)	1761
EcoICRI	GAGCTC	2 cut(s)	451, 561
EcoO109I	RGGNCCY	3 cut(s)	595, 649, 888
EcoO65I	GGTNACC	1 cut(s)	1761
EcoRI	GAATTC	1 cut(s)	1597
EcoRII	CCWGG	7 cut(s)	435, 453, 508, 537, 551, 675, 1684
EcoT14I	CCWWGG	3 cut(s)	51, 873, 1517
EcoT38I	GRGCYC	2 cut(s)	453, 563
EgeI	GGCGCC	1 cut(s)	924
EheI	GGCGCC	1 cut(s)	924
ErhI	CCWWGG	3 cut(s)	51, 873, 1517
Esp3I	CGTCTC	3 cut(s)	20, 90, 874
FaeI	CATG	7 cut(s)	55, 352, 1211, 1549, 1571, 1642, 1761
FalI	AAGNNNNNCTT	4 cut(s)	245, 277, 1478, 1510
FaqI	GGGAC	3 cut(s)	190, 901, 1151
FatI	CATG	7 cut(s)	51, 348, 1207, 1545, 1567, 1638, 1757
FauI	CCCGC	5 cut(s)	53, 75, 581, 780, 1370
FblI	GTMKAC	1 cut(s)	1218
FokI	GGATG	4 cut(s)	835, 1087, 1447, 1596
FriOI	GRGCYC	2 cut(s)	453, 563
FspBI	CTAG	4 cut(s)	534, 696, 1533, 1613
GlaI	GCGC	6 cut(s)	474, 482, 484, 501, 567, 924
GsaI	CCCAGC	1 cut(s)	635
GsuI	CTGGAG	1 cut(s)	389
HaeII	RGCGCY	2 cut(s)	569, 926
HgaI	GACGC	7 cut(s)	235, 251, 265, 361, 479, 522, 1455
HhaI	GCGC	6 cut(s)	475, 483, 485, 502, 568, 925
Hin1I	GRCGYC	2 cut(s)	243, 923
Hin1II	CATG	7 cut(s)	55, 352, 1211, 1549, 1571, 1642, 1761
Hin6I	GCGC	6 cut(s)	473, 481, 483, 500, 566, 923
HinP1I	GCGC	6 cut(s)	473, 481, 483, 500, 566, 923
HincII	GTYRAC	2 cut(s)	280, 1670
HindII	GTYRAC	2 cut(s)	280, 1670
HinfI	GANTC	4 cut(s)	256, 461, 724, 1420
HphI	GGTGA	5 cut(s)	72, 631, 676, 1643, 1720
Hpy188III	TCNNGA	5 cut(s)	406, 578, 714, 796, 1283
Hpy99I	CGWCG	8 cut(s)	229, 500, 521, 608, 1034, 1187, 1471, 1504
HpyAV	CCTTC	7 cut(s)	276, 352, 808, 963, 985, 993, 1114
HpyCH4IV	ACGT	9 cut(s)	543, 584, 606, 957, 1145, 1250, 1502, 1672, 1703
HpyCH4V	TGCA	7 cut(s)	358, 1133, 1227, 1261, 1328, 1463, 1736
HpyF3I	CTNAG	3 cut(s)	365, 557, 1380
HpySE526I	ACGT	9 cut(s)	543, 584, 606, 957, 1145, 1250, 1502, 1672, 1703
Hsp92I	GRCGYC	2 cut(s)	243, 923
Hsp92II	CATG	7 cut(s)	55, 352, 1211, 1549, 1571, 1642, 1761
HspAI	GCGC	6 cut(s)	473, 481, 483, 500, 566, 923
KasI	GGCGCC	1 cut(s)	922
KroI	GCCGGC	1 cut(s)	190
KroNI	GCCGGC	1 cut(s)	192
Kzo9I	GATC	9 cut(s)	182, 302, 900, 912, 1204, 1440, 1455, 1471, 1564
LmnI	GCTCC	8 cut(s)	44, 53, 254, 456, 628, 658, 1118, 1579
Lsp1109I	GCAGC	5 cut(s)	158, 342, 370, 1340, 1475
LweI	GCATC	1 cut(s)	1109
MaeI	CTAG	4 cut(s)	534, 696, 1533, 1613
MaeII	ACGT	9 cut(s)	543, 584, 606, 957, 1145, 1250, 1502, 1672, 1703
MaeIII	GTNAC	4 cut(s)	668, 701, 1289, 1761
MalI	GATC	9 cut(s)	184, 304, 902, 914, 1206, 1442, 1457, 1473, 1566
MbiI	CCGCTC	2 cut(s)	48, 612
MboI	GATC	9 cut(s)	182, 302, 900, 912, 1204, 1440, 1455, 1471, 1564
MboII	GAAGA	3 cut(s)	989, 992, 1015
MflI	RGATCY	3 cut(s)	302, 1455, 1471
MhlI	GDGCHC	3 cut(s)	453, 563, 1263
MlsI	TGGCCA	1 cut(s)	1684
MluCI	AATT	3 cut(s)	1345, 1597, 1603
MluNI	TGGCCA	1 cut(s)	1684
Mly113I	GGCGCC	1 cut(s)	923
MlyI	GAGTC	2 cut(s)	250, 455
MmeI	TCCRAC	6 cut(s)	43, 51, 636, 1290, 1449, 1523
Mox20I	TGGCCA	1 cut(s)	1684
MroNI	GCCGGC	1 cut(s)	190
MscI	TGGCCA	1 cut(s)	1684
MslI	CAYNNNNRTG	2 cut(s)	1433, 1775
Msp20I	TGGCCA	1 cut(s)	1684
MspA1I	CMGCKG	2 cut(s)	152, 1056
Mva1269I	GAATGC	1 cut(s)	1781
MvaI	CCWGG	7 cut(s)	437, 455, 510, 539, 553, 677, 1686
MvnI	CGCG	9 cut(s)	197, 473, 481, 483, 500, 516, 787, 1109, 1194
NaeI	GCCGGC	1 cut(s)	192
NarI	GGCGCC	1 cut(s)	923
NciI	CCSGG	6 cut(s)	33, 299, 300, 617, 1162, 1653
NcoI	CCATGG	1 cut(s)	51
NdeII	GATC	9 cut(s)	182, 302, 900, 912, 1204, 1440, 1455, 1471, 1564
NgoMIV	GCCGGC	1 cut(s)	190
NlaIII	CATG	7 cut(s)	55, 352, 1211, 1549, 1571, 1642, 1761
NlaIV	GGNNCC	5 cut(s)	40, 414, 889, 924, 1457
NmeAIII	GCCGAG	2 cut(s)	542, 931
NmuCI	GTSAC	1 cut(s)	1289
NspI	RCATGY	1 cut(s)	1549
OliI	CACNNNNGTG	1 cut(s)	1775
PaeR7I	CTCGAG	2 cut(s)	446, 1589
PauI	GCGCGC	1 cut(s)	481
PciI	ACATGT	1 cut(s)	1545
PcsI	WCGNNNNNNNCGW	4 cut(s)	395, 719, 1191, 1436
PctI	GAATGC	1 cut(s)	1781
PdiI	GCCGGC	1 cut(s)	192
PfeI	GAWTC	2 cut(s)	724, 1420
PflFI	GACNNNGTC	1 cut(s)	329
PfoI	TCCNGGA	1 cut(s)	453
PinAI	ACCGGT	1 cut(s)	1221
Ple19I	CGATCG	1 cut(s)	903
PleI	GAGTC	2 cut(s)	250, 455
PluTI	GGCGCC	1 cut(s)	926
PmaCI	CACGTG	1 cut(s)	585
PmlI	CACGTG	1 cut(s)	585
PpsI	GAGTC	2 cut(s)	250, 455
Ppu21I	YACGTR	1 cut(s)	585
PpuMI	RGGWCCY	2 cut(s)	649, 888
PscI	ACATGT	1 cut(s)	1545
PshAI	GACNNNNGTC	1 cut(s)	494
Psp124BI	GAGCTC	2 cut(s)	453, 563
Psp1406I	AACGTT	1 cut(s)	1145
Psp5II	RGGWCCY	2 cut(s)	649, 888
Psp6I	CCWGG	7 cut(s)	435, 453, 508, 537, 551, 675, 1684
PspCI	CACGTG	1 cut(s)	585
PspEI	GGTNACC	1 cut(s)	1761
PspFI	CCCAGC	1 cut(s)	631
PspGI	CCWGG	7 cut(s)	435, 453, 508, 537, 551, 675, 1684
PspN4I	GGNNCC	5 cut(s)	40, 414, 889, 924, 1457
PspPI	GGNCC	8 cut(s)	412, 595, 614, 634, 649, 888, 1163, 1649
PspPPI	RGGWCCY	2 cut(s)	649, 888
PspXI	VCTCGAGB	2 cut(s)	446, 1589
PstI	CTGCAG	1 cut(s)	360
PstNI	CAGNNNCTG	1 cut(s)	152
PsuI	RGATCY	3 cut(s)	302, 1455, 1471
PsyI	GACNNNGTC	1 cut(s)	329
PteI	GCGCGC	1 cut(s)	481
PvuI	CGATCG	1 cut(s)	903
RsaI	GTAC	4 cut(s)	852, 1303, 1529, 1544
RsaNI	GTAC	4 cut(s)	851, 1302, 1528, 1543
RseI	CAYNNNNRTG	2 cut(s)	1433, 1775
SacI	GAGCTC	2 cut(s)	453, 563
Sau3AI	GATC	9 cut(s)	182, 302, 900, 912, 1204, 1440, 1455, 1471, 1564
Sau96I	GGNCC	8 cut(s)	412, 595, 614, 634, 649, 888, 1163, 1649
SchI	GAGTC	2 cut(s)	250, 455
SduI	GDGCHC	3 cut(s)	453, 563, 1263
SfaNI	GCATC	1 cut(s)	1109
SfcI	CTRYAG	2 cut(s)	356, 1662
SfoI	GGCGCC	1 cut(s)	924
Sfr274I	CTCGAG	2 cut(s)	446, 1589
SinI	GGWCC	3 cut(s)	412, 649, 888
SlaI	CTCGAG	2 cut(s)	446, 1589
SmaI	CCCGGG	1 cut(s)	300
SmiMI	CAYNNNNRTG	2 cut(s)	1433, 1775
SmlI	CTYRAG	3 cut(s)	446, 644, 1589
SmoI	CTYRAG	3 cut(s)	446, 644, 1589
SpeI	ACTAGT	1 cut(s)	695
Sse9I	AATT	3 cut(s)	1345, 1597, 1603
SspDI	GGCGCC	1 cut(s)	922
SspMI	CTAG	4 cut(s)	534, 696, 1533, 1613
SstI	GAGCTC	2 cut(s)	453, 563
StyI	CCWWGG	3 cut(s)	51, 873, 1517
TaiI	ACGT	9 cut(s)	546, 587, 609, 960, 1148, 1253, 1505, 1675, 1706
TaqI	TCGA	4 cut(s)	291, 447, 1439, 1590
TaqII	GACCGA	2 cut(s)	338, 1789
TasI	AATT	3 cut(s)	1345, 1597, 1603
TatI	WGTACW	2 cut(s)	1527, 1542
TauI	GCSGC	7 cut(s)	73, 146, 152, 197, 615, 1112, 1168
TfiI	GAWTC	2 cut(s)	724, 1420
TseFI	GTSAC	1 cut(s)	1289
TseI	GCWGC	5 cut(s)	146, 355, 358, 1328, 1463
Tsp45I	GTSAC	1 cut(s)	1289
TspDTI	ATGAA	5 cut(s)	990, 1012, 1117, 1303, 1655
TspGWI	ACGGA	1 cut(s)	894
TspMI	CCCGGG	1 cut(s)	298
Tth111I	GACNNNGTC	1 cut(s)	329
VneI	GTGCAC	1 cut(s)	1259
VpaK11BI	GGWCC	3 cut(s)	412, 649, 888
XapI	RAATTY	1 cut(s)	1597
XceI	RCATGY	1 cut(s)	1549
XhoI	CTCGAG	2 cut(s)	446, 1589
XmaI	CCCGGG	1 cut(s)	298
XmiI	GTMKAC	1 cut(s)	1218
XspI	CTAG	4 cut(s)	534, 696, 1533, 1613

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.