Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007564

Physical Location & Seq

Genomic Coordinates Reverse (-)

35001093 .. 35005819

Gene Structure 4727 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1289200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGATTACGGCAGACTCAAACCGTCGGAGCCGGAAGGCTCCTCCAAGCACCACGTGGCGGAAAACCCATCACTCCCACAAACCGTCACGTCATCCTCCTCCTCAACCAGCCGCAGCCGCAAGAACAAGCTCATCCTAATCTCTCTCCTCTCCGTCGCACTCATACTCGCGTCCGCCGTCTCCGCAGTCGTCCTAGTCGCCGGCCGCTCCAAAGCTTCAAACTCCGCTGCCTCCTCCGCCATCCACCGCAAGCCCACGAAGGCCATCTCAGACGCGTGCGCCAAGACGCGCTTCCCCTCCCTCTGCGTCGACTCCCTACTCGACTTCCCCGGCTCCACCACCGCGTCGGAGCAGGACCTCGTCCACATTTCCTTCAACATGACGCTGCAGCGCCTCAGTAAGGCGCTCTACATCTCCTCCTCCCTCTCCTACCGCCAGATGGATCCCTACTCCCGCTCCGCCTACGATGACTGCCTCGAGCTCCTCGACGACTCCGTCGACGCCCTCTCCCGAGCCCTCACCTCCGTCGTCCCTGGTGCAGCGTCCACGTCCACACAGGACGTGCTGACGTGGCTGAGCGCAGCGCTCACGAACCAGGACACGTGCGGGGAGGGTTTTGAGCAAATCAATGGCGGCGACGTCAAGAACGAGATGGACCAGCGGCTTAAGGACTTGTCGGAACTCGTGAGCAACTGCCTCGCAATTTACTCGGCGATCGGGGGCGGCGATTTCTCCGGAGTCCCGATACAGAACAGGCGGAGGATGTTGTTGGGTAATTCCGACATGGAGCGCGATATAATGGGGGATAATGCCGATGTCTCGCGAGATTTTCCGAGGTGGTTGGGTAGGAGGGAGAGGAGGTTGCTGTCGGTGCCGGGATCGCAGATACAGGCGGATATTATCGTGTCGAAAGATGGCAGTGGGACGTTCAAGACGATCGCCGAGGCAATCAAAAAGGCGCCGGAGTCTAGTTCTCGTCGGACAATTATTTACGTCAAAGCAGGAAGGTACGAAGAGAGTAATTTGAAGGTGGCAAGGAAGAAGACCAACTTGATGTTCATCGGAGACGGGAAGGGCAAAACCATAATTACCGGAGGGAAAAACGTCGCGCAGAAAGTGACCACATTCCACACTGCAACCTTCGCTGCTCAAGGAGCTGGTTTTATTGCACGTGACATAACGTTCGAGAACTACGCGGGACCGGACAAGCACCAAGCCGTCGCCCTTCGAATAGGAGCGGACCACGCTGTGGTCTACCACTGCAGCATCATCGGACACCAAGACACATTTTACGTGCACTCGAATCGCCAATTCGTCCGTGAAACGGATATTTACGGGACAGTGGATTTCATTTTTGGCAATGCGGCTGTAGTGTTCCAAAAGTGTAGCCTCTACGCTCGCAAGCCCATGGCCAATCAAAAAAACACAATCACAGCCCAAAACCGGAAGGACCCGAATCAGAACACGGGCATTTCTATCCATGATTGCCGGATCCTCGCCACTCCGGAGCTCGAGGGTTCAAAGGGTAGCTTCCCGACCTATCTAGGCCGTCCGTGGAAGATGTACTCTAGGACAGTTTACATGTTATCGTTCATTGGTGATCACGTTCATCCGCGTGGATGGCTTGAGTGGAATGGGAATTTCGCCCTAGATACCCTATACTATGGTGAATACATGAACTCTGGCCCTGGCGCGGCGGTAGGGCAACGAGTAACTTGGCCTGGGTATCGGGTCATTACCTCTCCGGTGGAGGCCGGTAAGTTCACTGTTGCACAGTTTATATACGGGTCGTCATGGTTGCCGTCCACTGGGGTGGCATTCTTGGCCGGTTTATCAGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0010119 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050789 GO:0050790 GO:0050794 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

614

Amino Acids

66.83

Weight (kDa)

9.08

Isoelectric Point (pI)

34.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	88 - 235	1.7e-36	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	299 - 596	1e-143	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49220 AT3G49220 AT3G49220 AT5G53370 AT5G53370
fragaria_vesca	FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650 FvH4_7g31650
malus_domestica	MD01G1220700.v1.1 MD07G1289200.v1.1
prunus_persica	Prupe.2G310600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0380731
rosa_laevigata	RLG00000026273
rosa_multiflora	Rmu_sc0001637.1_g000012
rosa_roxburghii	Rroxscaffold_4G00279070
rosa_rugosa	Rorug01G0424000
rosa_samantha	Rh1AG448200 Rh1BG403600 Rh1CG417900 Rh1DG433900
rosa_wichuraiana	Rw0G005890 Rw1G039010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	494
AatII	GACGTC	1 cut(s)	642
AccB1I	GGYRCC	2 cut(s)	871, 958
AccB7I	CCANNNNNTGG	1 cut(s)	1249
AccBSI	CCGCTC	3 cut(s)	207, 456, 1238
AccI	GTMKAC	3 cut(s)	309, 498, 1254
AccIII	TCCGGA	2 cut(s)	734, 1504
AclI	AACGTT	1 cut(s)	1181
AclWI	GGATC	5 cut(s)	437, 450, 886, 1486, 1499
AcoI	YGGCCR	3 cut(s)	202, 1410, 1824
AcsI	RAATTY	1 cut(s)	1639
AcvI	CACGTG	3 cut(s)	55, 603, 1172
AcyI	GRCGYC	3 cut(s)	501, 639, 959
AdeI	CACNNNGTG	2 cut(s)	55, 1249
AfaI	GTAC	2 cut(s)	1010, 1565
AfeI	AGCGCT	1 cut(s)	585
AfiI	CCNNNNNNNGG	8 cut(s)	58, 400, 439, 1249, 1324, 1443, 1693, 1808
AflII	CTTAAG	1 cut(s)	665
AflIII	ACRYGT	3 cut(s)	273, 600, 1581
AgsI	TTSAA	5 cut(s)	219, 376, 931, 1027, 1521
AjiI	CACGTC	4 cut(s)	90, 549, 562, 570
AjnI	CCWGG	4 cut(s)	532, 594, 1687, 1720
AjuI	GAANNNNNNNTTGG	4 cut(s)	275, 307, 1040, 1072
AleI	CACNNNNGTG	1 cut(s)	1811
AluBI	AGCT	6 cut(s)	130, 215, 481, 1157, 1510, 1530
AluI	AGCT	6 cut(s)	130, 215, 481, 1157, 1510, 1530
Alw21I	GWGCWC	3 cut(s)	483, 1299, 1512
Alw26I	GTCTC	3 cut(s)	184, 823, 1059
Alw44I	GTGCAC	1 cut(s)	1295
AlwI	GGATC	5 cut(s)	437, 450, 886, 1486, 1499
Ama87I	CYCGRG	3 cut(s)	476, 510, 1511
Aor13HI	TCCGGA	2 cut(s)	734, 1504
Aor51HI	AGCGCT	1 cut(s)	585
AoxI	GGCC	8 cut(s)	202, 261, 1410, 1546, 1684, 1718, 1752, 1824
ApaLI	GTGCAC	1 cut(s)	1295
ApeKI	GCWGC	8 cut(s)	114, 227, 385, 388, 539, 581, 1145, 1263
ApoI	RAATTY	1 cut(s)	1639
ArsI	GACNNNNNNTTYG	2 cut(s)	1166, 1198
AspS9I	GGNCC	6 cut(s)	355, 655, 1199, 1240, 1450, 1685
AsuC2I	CCSGG	2 cut(s)	330, 876
AsuHPI	GGTGA	3 cut(s)	511, 1610, 1679
AsuII	TTCGAA	1 cut(s)	1228
AvaI	CYCGRG	3 cut(s)	476, 510, 1511
AvaII	GGWCC	5 cut(s)	355, 655, 1199, 1240, 1450
BaeGI	GKGCMC	1 cut(s)	1299
BalI	TGGCCA	1 cut(s)	1412
BamHI	GGATCC	2 cut(s)	442, 1491
BanI	GGYRCC	2 cut(s)	871, 958
BanII	GRGCYC	3 cut(s)	483, 517, 1512
BauI	CACGAG	1 cut(s)	683
BbrPI	CACGTG	3 cut(s)	55, 603, 1172
BbsI	GAAGAC	1 cut(s)	1049
Bbv12I	GWGCWC	3 cut(s)	483, 1299, 1512
BbvI	GCAGC	8 cut(s)	126, 214, 372, 400, 551, 593, 1132, 1275
BccI	CCATC	7 cut(s)	76, 248, 272, 433, 646, 908, 1614
BceAI	ACGGC	5 cut(s)	25, 161, 1202, 1533, 1786
BcgI	CGANNNNNNTGC	8 cut(s)	679, 690, 713, 724, 1252, 1286, 1286, 1320
BciT130I	CCWGG	4 cut(s)	534, 596, 1689, 1722
BclI	TGATCA	1 cut(s)	1600
BcnI	CCSGG	2 cut(s)	330, 876
BcoDI	GTCTC	3 cut(s)	184, 823, 1059
BfaI	CTAG	5 cut(s)	194, 969, 1544, 1569, 1649
BfmI	CTRYAG	3 cut(s)	386, 1261, 1368
BfoI	RGCGCY	4 cut(s)	394, 407, 587, 962
BfrI	CTTAAG	1 cut(s)	665
BlpI	GCTNAGC	1 cut(s)	575
Bme1390I	CCNGG	6 cut(s)	330, 534, 596, 876, 1689, 1722
Bme18I	GGWCC	5 cut(s)	355, 655, 1199, 1240, 1450
BmeT110I	CYCGRG	3 cut(s)	476, 510, 1511
BmgBI	CACGTC	4 cut(s)	90, 549, 562, 570
BmgT120I	GGNCC	6 cut(s)	355, 655, 1199, 1240, 1450, 1685
BmiI	GGNNCC	9 cut(s)	30, 40, 334, 444, 873, 960, 1200, 1452, 1493
BmrFI	CCNGG	6 cut(s)	330, 534, 596, 876, 1689, 1722
BmrI	ACTGGG	1 cut(s)	1818
BmsI	GCATC	1 cut(s)	1275
BmuI	ACTGGG	1 cut(s)	1818
BpiI	GAAGAC	1 cut(s)	1049
Bpu1102I	GCTNAGC	1 cut(s)	575
Bpu14I	TTCGAA	1 cut(s)	1228
BpuEI	CTTGAG	2 cut(s)	1134, 1646
BpuMI	CCSGG	2 cut(s)	330, 876
BsaAI	YACGTR	4 cut(s)	55, 603, 1172, 1294
BsaBI	GATNNNNATC	1 cut(s)	1058
BsaHI	GRCGYC	3 cut(s)	501, 639, 959
BsaJI	CCNNGG	8 cut(s)	328, 532, 833, 942, 1407, 1553, 1687, 1721
BsaWI	WCCGGW	6 cut(s)	734, 1091, 1201, 1443, 1504, 1744
BsaXI	ACNNNNNCTCC	8 cut(s)	401, 431, 440, 470, 491, 521, 845, 875
Bsc4I	CCNNNNNNNGG	8 cut(s)	58, 400, 439, 1249, 1324, 1443, 1693, 1808
Bse118I	RCCGGY	3 cut(s)	200, 1754, 1826
Bse1I	ACTGG	1 cut(s)	1813
Bse3DI	GCAATG	1 cut(s)	1366
Bse8I	GATNNNNATC	1 cut(s)	1058
BseAI	TCCGGA	2 cut(s)	734, 1504
BseBI	CCWGG	4 cut(s)	534, 596, 1689, 1722
BseDI	CCNNGG	8 cut(s)	328, 532, 833, 942, 1407, 1553, 1687, 1721
BseGI	GGATG	6 cut(s)	92, 132, 240, 768, 1609, 1625
BseJI	GATNNNNATC	1 cut(s)	1058
BseLI	CCNNNNNNNGG	8 cut(s)	58, 400, 439, 1249, 1324, 1443, 1693, 1808
BseMI	GCAATG	1 cut(s)	1366
BseMII	CTCAG	3 cut(s)	282, 409, 566
BseNI	ACTGG	1 cut(s)	1813
BseRI	GAGGAG	9 cut(s)	31, 88, 91, 137, 223, 406, 409, 473, 871
BseSI	GKGCMC	1 cut(s)	1299
BseX3I	CGGCCG	1 cut(s)	202
BseXI	GCAGC	8 cut(s)	126, 214, 372, 400, 551, 593, 1132, 1275
BsgI	GTGCAG	1 cut(s)	558
Bsh1285I	CGRYCG	3 cut(s)	205, 717, 939
BshFI	GGCC	8 cut(s)	204, 263, 1412, 1548, 1686, 1720, 1754, 1826
BshNI	GGYRCC	2 cut(s)	871, 958
BsiEI	CGRYCG	3 cut(s)	205, 717, 939
BsiHKAI	GWGCWC	3 cut(s)	483, 1299, 1512
BsiHKCI	CYCGRG	3 cut(s)	476, 510, 1511
BslFI	GGGAC	5 cut(s)	515, 725, 937, 1212, 1351
BslI	CCNNNNNNNGG	8 cut(s)	58, 400, 439, 1249, 1324, 1443, 1693, 1808
BsmAI	GTCTC	3 cut(s)	184, 823, 1059
BsmBI	CGTCTC	2 cut(s)	184, 1059
BsmFI	GGGAC	5 cut(s)	515, 725, 937, 1212, 1351
BsmI	GAATGC	1 cut(s)	1817
BsnI	GGCC	8 cut(s)	204, 263, 1412, 1548, 1686, 1720, 1754, 1826
BsoBI	CYCGRG	3 cut(s)	476, 510, 1511
Bsp119I	TTCGAA	1 cut(s)	1228
Bsp1286I	GDGCHC	4 cut(s)	483, 517, 1299, 1512
Bsp13I	TCCGGA	2 cut(s)	734, 1504
Bsp143I	GATC	6 cut(s)	442, 714, 878, 936, 1491, 1600
Bsp1720I	GCTNAGC	1 cut(s)	575
Bsp19I	CCATGG	1 cut(s)	1407
Bsp68I	TCGCGA	1 cut(s)	823
BspANI	GGCC	8 cut(s)	204, 263, 1412, 1548, 1686, 1720, 1754, 1826
BspCNI	CTCAG	3 cut(s)	281, 408, 567
BspEI	TCCGGA	2 cut(s)	734, 1504
BspLI	GGNNCC	9 cut(s)	30, 40, 334, 444, 873, 960, 1200, 1452, 1493
BspMAI	CTGCAG	2 cut(s)	390, 1265
BspPI	GGATC	5 cut(s)	437, 450, 886, 1486, 1499
BspT104I	TTCGAA	1 cut(s)	1228
BspT107I	GGYRCC	2 cut(s)	871, 958
BspTI	CTTAAG	1 cut(s)	665
BsrBI	CCGCTC	3 cut(s)	207, 456, 1238
BsrDI	GCAATG	1 cut(s)	1366
BsrFI	RCCGGY	3 cut(s)	200, 1754, 1826
BsrI	ACTGG	1 cut(s)	1813
BssAI	RCCGGY	3 cut(s)	200, 1754, 1826
BssECI	CCNNGG	8 cut(s)	328, 532, 833, 942, 1407, 1553, 1687, 1721
BssMI	GATC	6 cut(s)	442, 714, 878, 936, 1491, 1600
BssNI	GRCGYC	3 cut(s)	501, 639, 959
BssSI	CACGAG	1 cut(s)	683
BssT1I	CCWWGG	1 cut(s)	1407
Bst2BI	CACGAG	1 cut(s)	683
Bst2UI	CCWGG	4 cut(s)	534, 596, 1689, 1722
Bst4CI	ACNGT	6 cut(s)	24, 85, 1342, 1576, 1768, 1776
Bst6I	CTCTTC	1 cut(s)	1008
BstACI	GRCGYC	3 cut(s)	501, 639, 959
BstAFI	CTTAAG	1 cut(s)	665
BstBAI	YACGTR	4 cut(s)	55, 603, 1172, 1294
BstBI	TTCGAA	1 cut(s)	1228
BstC8I	GCNNGC	5 cut(s)	202, 251, 277, 1399, 1403
BstDEI	CTNAG	3 cut(s)	268, 395, 575
BstDSI	CCRYGG	2 cut(s)	1407, 1553
BstENI	CCTNNNNNAGG	1 cut(s)	398
BstF5I	GGATG	6 cut(s)	92, 132, 240, 768, 1609, 1625
BstH2I	RGCGCY	4 cut(s)	394, 407, 587, 962
BstKTI	GATC	6 cut(s)	445, 717, 881, 939, 1494, 1603
BstMAI	GTCTC	3 cut(s)	184, 823, 1059
BstMBI	GATC	6 cut(s)	442, 714, 878, 936, 1491, 1600
BstMCI	CGRYCG	3 cut(s)	205, 717, 939
BstNI	CCWGG	4 cut(s)	534, 596, 1689, 1722
BstNSI	RCATGY	1 cut(s)	1585
BstSCI	CCNGG	6 cut(s)	328, 532, 594, 874, 1687, 1720
BstSFI	CTRYAG	3 cut(s)	386, 1261, 1368
BstSLI	GKGCMC	1 cut(s)	1299
BstV1I	GCAGC	8 cut(s)	126, 214, 372, 400, 551, 593, 1132, 1275
BstV2I	GAAGAC	1 cut(s)	1049
BstX2I	RGATCY	2 cut(s)	442, 1491
BstXI	CCANNNNNNTGG	1 cut(s)	1813
BstYI	RGATCY	2 cut(s)	442, 1491
BstZI	CGGCCG	1 cut(s)	202
BsuRI	GGCC	8 cut(s)	204, 263, 1412, 1548, 1686, 1720, 1754, 1826
BtgI	CCRYGG	2 cut(s)	1407, 1553
BtrI	CACGTC	4 cut(s)	90, 549, 562, 570
BtsCI	GGATG	6 cut(s)	92, 132, 240, 768, 1609, 1625
BtsI	GCAGTG	3 cut(s)	925, 1131, 1258
BtsIMutI	CAGTG	6 cut(s)	925, 1131, 1258, 1347, 1764, 1806
BtuMI	TCGCGA	1 cut(s)	823
Cac8I	GCNNGC	5 cut(s)	202, 251, 277, 1399, 1403
Cfr10I	RCCGGY	3 cut(s)	200, 1754, 1826
Cfr13I	GGNCC	6 cut(s)	355, 655, 1199, 1240, 1450, 1685
CseI	GACGC	8 cut(s)	159, 281, 295, 295, 333, 391, 509, 531
Csp6I	GTAC	2 cut(s)	1009, 1564
CviAII	CATG	7 cut(s)	379, 784, 1408, 1481, 1582, 1675, 1794
CviQI	GTAC	2 cut(s)	1009, 1564
DdeI	CTNAG	3 cut(s)	268, 395, 575
DinI	GGCGCC	1 cut(s)	960
DpnI	GATC	6 cut(s)	444, 716, 880, 938, 1493, 1602
DpnII	GATC	6 cut(s)	442, 714, 878, 936, 1491, 1600
DraIII	CACNNNGTG	2 cut(s)	55, 1249
DrdI	GACNNNNNNGTC	1 cut(s)	494
DseDI	GACNNNNNNGTC	1 cut(s)	494
EaeI	YGGCCR	3 cut(s)	202, 1410, 1824
EagI	CGGCCG	1 cut(s)	202
Eam1104I	CTCTTC	1 cut(s)	1008
EarI	CTCTTC	1 cut(s)	1008
EciI	GGCGGA	6 cut(s)	74, 163, 226, 448, 772, 908
Ecl136II	GAGCTC	2 cut(s)	481, 1510
EclXI	CGGCCG	1 cut(s)	202
Eco130I	CCWWGG	1 cut(s)	1407
Eco24I	GRGCYC	3 cut(s)	483, 517, 1512
Eco47I	GGWCC	5 cut(s)	355, 655, 1199, 1240, 1450
Eco47III	AGCGCT	1 cut(s)	585
Eco52I	CGGCCG	1 cut(s)	202
Eco53kI	GAGCTC	2 cut(s)	481, 1510
Eco72I	CACGTG	3 cut(s)	55, 603, 1172
Eco88I	CYCGRG	3 cut(s)	476, 510, 1511
EcoICRI	GAGCTC	2 cut(s)	481, 1510
EcoNI	CCTNNNNNAGG	1 cut(s)	398
EcoO109I	RGGNCCY	2 cut(s)	355, 1450
EcoRII	CCWGG	4 cut(s)	532, 594, 1687, 1720
EcoT14I	CCWWGG	1 cut(s)	1407
EcoT38I	GRGCYC	3 cut(s)	483, 517, 1512
EgeI	GGCGCC	1 cut(s)	960
EheI	GGCGCC	1 cut(s)	960
ErhI	CCWWGG	1 cut(s)	1407
Esp3I	CGTCTC	2 cut(s)	184, 1059
FaeI	CATG	7 cut(s)	382, 787, 1411, 1484, 1585, 1678, 1797
FalI	AAGNNNNNCTT	6 cut(s)	275, 307, 1034, 1066, 1514, 1546
FaqI	GGGAC	5 cut(s)	515, 725, 937, 1212, 1351
FatI	CATG	7 cut(s)	378, 783, 1407, 1480, 1581, 1674, 1793
FauI	CCCGC	3 cut(s)	461, 599, 1189
FbaI	TGATCA	1 cut(s)	1600
FblI	GTMKAC	3 cut(s)	309, 498, 1254
FokI	GGATG	6 cut(s)	79, 119, 227, 775, 1596, 1632
FriOI	GRGCYC	3 cut(s)	483, 517, 1512
FspBI	CTAG	5 cut(s)	194, 969, 1544, 1569, 1649
HaeII	RGCGCY	4 cut(s)	394, 407, 587, 962
HaeIII	GGCC	8 cut(s)	204, 263, 1412, 1548, 1686, 1720, 1754, 1826
HgaI	GACGC	8 cut(s)	159, 281, 295, 295, 333, 391, 509, 531
Hin1I	GRCGYC	3 cut(s)	501, 639, 959
Hin1II	CATG	7 cut(s)	382, 787, 1411, 1484, 1585, 1678, 1797
HincII	GTYRAC	2 cut(s)	310, 499
HindII	GTYRAC	2 cut(s)	310, 499
HindIII	AAGCTT	1 cut(s)	213
HinfI	GANTC	7 cut(s)	15, 311, 491, 738, 965, 1303, 1456
HphI	GGTGA	3 cut(s)	511, 1610, 1679
HpyAV	CCTTC	9 cut(s)	29, 253, 382, 999, 1021, 1066, 1150, 1235, 1441
HpyCH4III	ACNGT	6 cut(s)	24, 85, 1342, 1576, 1768, 1776
HpyCH4V	TGCA	7 cut(s)	388, 539, 1136, 1169, 1263, 1297, 1772
HpyF3I	CTNAG	3 cut(s)	268, 395, 575
Hsp92I	GRCGYC	3 cut(s)	501, 639, 959
Hsp92II	CATG	7 cut(s)	382, 787, 1411, 1484, 1585, 1678, 1797
KasI	GGCGCC	1 cut(s)	958
Kpn2I	TCCGGA	2 cut(s)	734, 1504
KroI	GCCGGC	1 cut(s)	200
KroNI	GCCGGC	1 cut(s)	202
Ksp22I	TGATCA	1 cut(s)	1600
Kzo9I	GATC	6 cut(s)	442, 714, 878, 936, 1491, 1600
Lsp1109I	GCAGC	8 cut(s)	126, 214, 372, 400, 551, 593, 1132, 1275
LweI	GCATC	1 cut(s)	1275
MaeI	CTAG	5 cut(s)	194, 969, 1544, 1569, 1649
MaeIII	GTNAC	4 cut(s)	85, 1117, 1172, 1711
MalI	GATC	6 cut(s)	444, 716, 880, 938, 1493, 1602
MbiI	CCGCTC	3 cut(s)	207, 456, 1238
MboI	GATC	6 cut(s)	442, 714, 878, 936, 1491, 1600
MboII	GAAGA	4 cut(s)	1025, 1051, 1054, 1570
MflI	RGATCY	2 cut(s)	442, 1491
MhlI	GDGCHC	4 cut(s)	483, 517, 1299, 1512
MlsI	TGGCCA	1 cut(s)	1412
MluCI	AATT	7 cut(s)	702, 775, 984, 1021, 1086, 1310, 1639
MluI	ACGCGT	1 cut(s)	273
MluNI	TGGCCA	1 cut(s)	1412
Mly113I	GGCGCC	1 cut(s)	959
MlyI	GAGTC	5 cut(s)	9, 305, 485, 747, 974
MmeI	TCCRAC	5 cut(s)	6, 327, 657, 804, 959
Mox20I	TGGCCA	1 cut(s)	1412
MroI	TCCGGA	2 cut(s)	734, 1504
MroNI	GCCGGC	1 cut(s)	200
MscI	TGGCCA	1 cut(s)	1412
MseI	TTAA	1 cut(s)	666
MslI	CAYNNNNRTG	2 cut(s)	1614, 1811
Msp20I	TGGCCA	1 cut(s)	1412
MspA1I	CMGCKG	2 cut(s)	227, 661
MspCI	CTTAAG	1 cut(s)	665
MspR9I	CCNGG	6 cut(s)	330, 534, 596, 876, 1689, 1722
MteI	GCGCNGCGC	1 cut(s)	582
Mva1269I	GAATGC	1 cut(s)	1817
MvaI	CCWGG	4 cut(s)	534, 596, 1689, 1722
NaeI	GCCGGC	1 cut(s)	202
NarI	GGCGCC	1 cut(s)	959
NciI	CCSGG	2 cut(s)	330, 876
NcoI	CCATGG	1 cut(s)	1407
NdeII	GATC	6 cut(s)	442, 714, 878, 936, 1491, 1600
NgoMIV	GCCGGC	1 cut(s)	200
NlaIII	CATG	7 cut(s)	382, 787, 1411, 1484, 1585, 1678, 1797
NlaIV	GGNNCC	9 cut(s)	30, 40, 334, 444, 873, 960, 1200, 1452, 1493
NmeAIII	GCCGAG	2 cut(s)	689, 967
NmuCI	GTSAC	3 cut(s)	85, 1117, 1172
NruI	TCGCGA	1 cut(s)	823
NspI	RCATGY	1 cut(s)	1585
NspV	TTCGAA	1 cut(s)	1228
OliI	CACNNNNGTG	1 cut(s)	1811
PaeR7I	CTCGAG	2 cut(s)	476, 1511
PciI	ACATGT	1 cut(s)	1581
PcsI	WCGNNNNNNNCGW	7 cut(s)	174, 483, 492, 495, 645, 723, 932
PctI	GAATGC	1 cut(s)	1817
PdiI	GCCGGC	1 cut(s)	202
PfeI	GAWTC	2 cut(s)	1303, 1456
PflFI	GACNNNGTC	2 cut(s)	359, 494
PflMI	CCANNNNNTGG	1 cut(s)	1249
Ple19I	CGATCG	2 cut(s)	717, 939
PleI	GAGTC	5 cut(s)	9, 305, 485, 746, 973
PluTI	GGCGCC	1 cut(s)	962
PmaCI	CACGTG	3 cut(s)	55, 603, 1172
PmlI	CACGTG	3 cut(s)	55, 603, 1172
PpsI	GAGTC	5 cut(s)	9, 305, 485, 746, 973
Ppu21I	YACGTR	4 cut(s)	55, 603, 1172, 1294
PpuMI	RGGWCCY	2 cut(s)	355, 1450
PscI	ACATGT	1 cut(s)	1581
Psp124BI	GAGCTC	2 cut(s)	483, 1512
Psp1406I	AACGTT	1 cut(s)	1181
Psp5II	RGGWCCY	2 cut(s)	355, 1450
Psp6I	CCWGG	4 cut(s)	532, 594, 1687, 1720
PspCI	CACGTG	3 cut(s)	55, 603, 1172
PspGI	CCWGG	4 cut(s)	532, 594, 1687, 1720
PspN4I	GGNNCC	9 cut(s)	30, 40, 334, 444, 873, 960, 1200, 1452, 1493
PspPI	GGNCC	6 cut(s)	355, 655, 1199, 1240, 1450, 1685
PspPPI	RGGWCCY	2 cut(s)	355, 1450
PspXI	VCTCGAGB	2 cut(s)	476, 1511
PstI	CTGCAG	2 cut(s)	390, 1265
PsuI	RGATCY	2 cut(s)	442, 1491
PsyI	GACNNNGTC	2 cut(s)	359, 494
PvuI	CGATCG	2 cut(s)	717, 939
RruI	TCGCGA	1 cut(s)	823
RsaI	GTAC	2 cut(s)	1010, 1565
RsaNI	GTAC	2 cut(s)	1009, 1564
RseI	CAYNNNNRTG	2 cut(s)	1614, 1811
SacI	GAGCTC	2 cut(s)	483, 1512
SalI	GTCGAC	2 cut(s)	308, 497
SaqAI	TTAA	1 cut(s)	666
Sau3AI	GATC	6 cut(s)	442, 714, 878, 936, 1491, 1600
Sau96I	GGNCC	6 cut(s)	355, 655, 1199, 1240, 1450, 1685
SchI	GAGTC	5 cut(s)	9, 305, 485, 747, 974
ScrFI	CCNGG	6 cut(s)	330, 534, 596, 876, 1689, 1722
SduI	GDGCHC	4 cut(s)	483, 517, 1299, 1512
SfaNI	GCATC	1 cut(s)	1275
SfcI	CTRYAG	3 cut(s)	386, 1261, 1368
SfoI	GGCGCC	1 cut(s)	960
Sfr274I	CTCGAG	2 cut(s)	476, 1511
SfuI	TTCGAA	1 cut(s)	1228
SgrDI	CGTCGACG	1 cut(s)	497
SinI	GGWCC	5 cut(s)	355, 655, 1199, 1240, 1450
SlaI	CTCGAG	2 cut(s)	476, 1511
SmiMI	CAYNNNNRTG	2 cut(s)	1614, 1811
SmlI	CTYRAG	5 cut(s)	476, 665, 1149, 1511, 1625
SmoI	CTYRAG	5 cut(s)	476, 665, 1149, 1511, 1625
Sse9I	AATT	7 cut(s)	702, 775, 984, 1021, 1086, 1310, 1639
SspDI	GGCGCC	1 cut(s)	958
SspMI	CTAG	5 cut(s)	194, 969, 1544, 1569, 1649
SstI	GAGCTC	2 cut(s)	483, 1512
StyD4I	CCNGG	6 cut(s)	328, 532, 594, 874, 1687, 1720
StyI	CCWWGG	1 cut(s)	1407
TaaI	ACNGT	6 cut(s)	24, 85, 1342, 1576, 1768, 1776
TasI	AATT	7 cut(s)	702, 775, 984, 1021, 1086, 1310, 1639
TatI	WGTACW	1 cut(s)	1563
TauI	GCSGC	8 cut(s)	114, 120, 207, 636, 664, 726, 1367, 1697
TfiI	GAWTC	2 cut(s)	1303, 1456
Tru1I	TTAA	1 cut(s)	666
Tru9I	TTAA	1 cut(s)	666
TscAI	CASTG	6 cut(s)	925, 1138, 1265, 1347, 1771, 1813
TseFI	GTSAC	3 cut(s)	85, 1117, 1172
TseI	GCWGC	8 cut(s)	114, 227, 385, 388, 539, 581, 1145, 1263
Tsp45I	GTSAC	3 cut(s)	85, 1117, 1172
TspDTI	ATGAA	5 cut(s)	1048, 1339, 1582, 1598, 1691
TspGWI	ACGGA	6 cut(s)	142, 484, 514, 1307, 1340, 1542
TspRI	CASTG	6 cut(s)	925, 1138, 1265, 1347, 1771, 1813
Tth111I	GACNNNGTC	2 cut(s)	359, 494
Van91I	CCANNNNNTGG	1 cut(s)	1249
Vha464I	CTTAAG	1 cut(s)	665
VneI	GTGCAC	1 cut(s)	1295
VpaK11BI	GGWCC	5 cut(s)	355, 655, 1199, 1240, 1450
XagI	CCTNNNNNAGG	1 cut(s)	398
XapI	RAATTY	1 cut(s)	1639
XceI	RCATGY	1 cut(s)	1585
XcmI	CCANNNNNNNNNTGG	1 cut(s)	52
XhoI	CTCGAG	2 cut(s)	476, 1511
XmiI	GTMKAC	3 cut(s)	309, 498, 1254
XspI	CTAG	5 cut(s)	194, 969, 1544, 1569, 1649
ZraI	GACGTC	1 cut(s)	640

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.